Lag0029883 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0029883
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A
Location	chr8: 42874885 .. 42875364 (+)
RNA-Seq Expression	Lag0029883
Synteny	Lag0029883

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAACTCTTTTCCCTAGTCTACTGCATCTCGAAGAATTTCGTCGTCGGAGCTTCTTGTTGTCTTTGTGTTCTTCTCTCAGTCGATCAGATCCGAAGGTACAGTCATCTTCTTCTTTTCATCTCGAATTGTGATTCGAAGATTTTATCAACCTTTATCGGTTCAGGATTAGAATTTCGTTTTCTTAGCAAAATTGGATTCCTAATCTCTGGACCTGTGAATCGGTCTCCCAAACTGTTTGTGAATGAGTACGTTTTGCTGTATAGTGTTTGAAGAATTTTAAATTTCCAGTTTTCTGTGCTGAAATTTGAAATGATTGTTTGCGTTCGAAGAATTTGATTTCCGTCGTGTTGATGCGCAGCGCAGATGATTGACGATCAGGCGTTAGGTTTTCTTGCCAATTTTCTCGGCATTTTTATCTTTGCATTGGTGATTGCCTACCATTACGTTACGGCAGATCCGAAGTATGAAGGCAACTGA

mRNA sequence

ATGAAACTCTTTTCCCTAGTCTACTGCATCTCGAAGAATTTCGTCGTCGGAGCTTCTTGTTGTCTTTGTGTTCTTCTCTCAGTCGATCAGATCCGAAGGTACAGTCATCTTCTTCTTTTCATCTCGAATTGTGATTCGAAGATTTTATCAACCTTTATCGGTTCAGGATTAGAATTTCGTTTTCTTAGCAAAATTGGATTCCTAATCTCTGGACCTGTGAATCGGTCTCCCAAACTGTTTGTGAATGAGTACGTTTTGCTGTATACGCAGATGATTGACGATCAGGCGTTAGGTTTTCTTGCCAATTTTCTCGGCATTTTTATCTTTGCATTGGTGATTGCCTACCATTACGTTACGGCAGATCCGAAGTATGAAGGCAACTGA

Coding sequence (CDS)

ATGAAACTCTTTTCCCTAGTCTACTGCATCTCGAAGAATTTCGTCGTCGGAGCTTCTTGTTGTCTTTGTGTTCTTCTCTCAGTCGATCAGATCCGAAGGTACAGTCATCTTCTTCTTTTCATCTCGAATTGTGATTCGAAGATTTTATCAACCTTTATCGGTTCAGGATTAGAATTTCGTTTTCTTAGCAAAATTGGATTCCTAATCTCTGGACCTGTGAATCGGTCTCCCAAACTGTTTGTGAATGAGTACGTTTTGCTGTATACGCAGATGATTGACGATCAGGCGTTAGGTTTTCTTGCCAATTTTCTCGGCATTTTTATCTTTGCATTGGTGATTGCCTACCATTACGTTACGGCAGATCCGAAGTATGAAGGCAACTGA

Protein sequence

MKLFSLVYCISKNFVVGASCCLCVLLSVDQIRRYSHLLLFISNCDSKILSTFIGSGLEFRFLSKIGFLISGPVNRSPKLFVNEYVLLYTQMIDDQALGFLANFLGIFIFALVIAYHYVTADPKYEGN

Homology

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BLAST of Lag0029883 vs. NCBI nr
Match: KAG7033043.1 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 90.9 bits (224), Expect = 8.6e-15
Identity = 47/65 (72.31%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 69  ISGPVNRSPKLFVNEYVLLYT------QMIDDQALGFLANFLGIFIFALVIAYHYVTADP 128
           ISGP N S +LFVN YV + +       MIDDQALGFLANFLGIF+FALVIAYHYV ADP
Sbjct: 5   ISGPGNSSSELFVNYYVAMSSFDESEMIMIDDQALGFLANFLGIFVFALVIAYHYVAADP 64

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BLAST of Lag0029883 vs. NCBI nr
Match: TYG35935.1 (hypothetical protein ES288_D13G024000v1 [Gossypium darwinii])

HSP 1 Score: 79.0 bits (193), Expect = 3.4e-11
Identity = 40/56 (71.43%), Postives = 44/56 (78.57%), Query Frame = 0


Query: 72  PVNRSPKLFVNEYVLLYTQMIDDQALGFLANFLGIFIFALVIAYHYVTADPKYEGN 128
           PVN  P  F++    +  QMIDD+ LGFLANFLGIFIFALVIAYHYV ADPKYEGN
Sbjct: 25  PVN--PVFFLSLKFYVVMQMIDDEQLGFLANFLGIFIFALVIAYHYVMADPKYEGN 78

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BLAST of Lag0029883 vs. NCBI nr
Match: KAB1993321.1 (hypothetical protein ES319_D13G022900v1 [Gossypium barbadense])

HSP 1 Score: 79.0 bits (193), Expect = 3.4e-11
Identity = 40/56 (71.43%), Postives = 44/56 (78.57%), Query Frame = 0


Query: 72  PVNRSPKLFVNEYVLLYTQMIDDQALGFLANFLGIFIFALVIAYHYVTADPKYEGN 128
           PVN  P  F++    +  QMIDD+ LGFLANFLGIFIFALVIAYHYV ADPKYEGN
Sbjct: 28  PVN--PVFFLSLKFYVVMQMIDDEQLGFLANFLGIFIFALVIAYHYVMADPKYEGN 81

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BLAST of Lag0029883 vs. NCBI nr
Match: KAB2047084.1 (hypothetical protein ES319_A13G021900v1 [Gossypium barbadense] >KAG4164492.1 hypothetical protein ERO13_A13G019700v2 [Gossypium hirsutum] >TYG85022.1 hypothetical protein ES288_A13G019600v1 [Gossypium darwinii])

HSP 1 Score: 77.8 bits (190), Expect = 7.5e-11
Identity = 37/52 (71.15%), Postives = 42/52 (80.77%), Query Frame = 0


Query: 76  SPKLFVNEYVLLYTQMIDDQALGFLANFLGIFIFALVIAYHYVTADPKYEGN 128
           +P  F++    +  QMIDD+ LGFLANFLGIFIFALVIAYHYV ADPKYEGN
Sbjct: 27  NPVFFLSLKFYVVMQMIDDEQLGFLANFLGIFIFALVIAYHYVMADPKYEGN 78

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BLAST of Lag0029883 vs. NCBI nr
Match: TYI99485.1 (hypothetical protein E1A91_A13G021200v1 [Gossypium mustelinum])

HSP 1 Score: 77.8 bits (190), Expect = 7.5e-11
Identity = 37/52 (71.15%), Postives = 42/52 (80.77%), Query Frame = 0


Query: 76  SPKLFVNEYVLLYTQMIDDQALGFLANFLGIFIFALVIAYHYVTADPKYEGN 128
           +P  F++    +  QMIDD+ LGFLANFLGIFIFALVIAYHYV ADPKYEGN
Sbjct: 27  NPVFFLSLKFYVVMQMIDDEQLGFLANFLGIFIFALVIAYHYVMADPKYEGN 78

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BLAST of Lag0029883 vs. ExPASy Swiss-Prot
Match: Q9LHK3 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Arabidopsis thaliana OX=3702 GN=OST4A PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.2e-11
Identity = 33/35 (94.29%), Postives = 34/35 (97.14%), Query Frame = 0


Query: 91  MIDDQALGFLANFLGIFIFALVIAYHYVTADPKYE 126
           MIDDQ LGF+ANFLGIFIFALVIAYHYVTADPKYE
Sbjct: 1   MIDDQDLGFIANFLGIFIFALVIAYHYVTADPKYE 35

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BLAST of Lag0029883 vs. ExPASy Swiss-Prot
Match: C7J4U3 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Oryza sativa subsp. japonica OX=39947 GN=OST4A PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 7.8e-11
Identity = 31/37 (83.78%), Postives = 32/37 (86.49%), Query Frame = 0


Query: 91  MIDDQALGFLANFLGIFIFALVIAYHYVTADPKYEGN 128
           M DDQ LGF ANFLGIFIF LVIAYH+V ADPKYEGN
Sbjct: 1   MFDDQDLGFFANFLGIFIFVLVIAYHFVMADPKYEGN 37

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BLAST of Lag0029883 vs. ExPASy Swiss-Prot
Match: C7J0R5 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4B OS=Oryza sativa subsp. japonica OX=39947 GN=OST4B PE=3 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 1.9e-09
Identity = 29/37 (78.38%), Postives = 31/37 (83.78%), Query Frame = 0


Query: 91  MIDDQALGFLANFLGIFIFALVIAYHYVTADPKYEGN 128
           M DDQ LGF ANFLGIFIF LV+AYH+V AD KYEGN
Sbjct: 1   MFDDQDLGFFANFLGIFIFVLVMAYHFVMADVKYEGN 37

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BLAST of Lag0029883 vs. ExPASy Swiss-Prot
Match: Q9SF57 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4C OS=Arabidopsis thaliana OX=3702 GN=OST4C PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 9.6e-09
Identity = 28/35 (80.00%), Postives = 30/35 (85.71%), Query Frame = 0


Query: 91  MIDDQALGFLANFLGIFIFALVIAYHYVTADPKYE 126
           M DDQ LGF ANFLGIFIF LVIAYH+V ADPK+E
Sbjct: 1   MFDDQDLGFFANFLGIFIFILVIAYHFVMADPKFE 35

[Close]

BLAST of Lag0029883 vs. ExPASy Swiss-Prot
Match: Q8L986 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4B OS=Arabidopsis thaliana OX=3702 GN=OST4B PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 3.6e-08
Identity = 26/35 (74.29%), Postives = 30/35 (85.71%), Query Frame = 0


Query: 91  MIDDQALGFLANFLGIFIFALVIAYHYVTADPKYE 126
           M DDQ LGF ANFLGIFIF +VIAYH+V A+PK+E
Sbjct: 1   MFDDQDLGFFANFLGIFIFIMVIAYHFVVAEPKFE 35

The following BLAST results are available for this feature:

BLAST of Lag0029883 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7033043.1	8.6e-15	72.31	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A, parti...	[more]
TYG35935.1	3.4e-11	71.43	hypothetical protein ES288_D13G024000v1 [Gossypium darwinii]	[more]
KAB1993321.1	3.4e-11	71.43	hypothetical protein ES319_D13G022900v1 [Gossypium barbadense]	[more]
KAB2047084.1	7.5e-11	71.15	hypothetical protein ES319_A13G021900v1 [Gossypium barbadense] >KAG4164492.1 hyp...	[more]
TYI99485.1	7.5e-11	71.15	hypothetical protein E1A91_A13G021200v1 [Gossypium mustelinum]	[more]

BLAST of Lag0029883 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LHK3	1.2e-11	94.29	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Ara...	[more]
C7J4U3	7.8e-11	83.78	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Ory...	[more]
C7J0R5	1.9e-09	78.38	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4B OS=Ory...	[more]
Q9SF57	9.6e-09	80.00	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4C OS=Ara...	[more]
Q8L986	3.6e-08	74.29	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4B OS=Ara...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018943	Oligosaccaryltransferase	PFAM	PF10215	Ost4	coord: 91..123 e-value: 3.3E-14 score: 52.4
IPR044165	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4, plant	PANTHER	PTHR28677	DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 4A-RELATED	coord: 86..127
IPR036330	Oligosaccharyltransferase subunit ost4p superfamily	SUPERFAMILY	103464	Oligosaccharyltransferase subunit ost4p	coord: 91..121

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0029883.1	Lag0029883.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016740	transferase activity