Lag0029716 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0029716 vs. NCBI nr
Match: XP_023551934.1 (uncharacterized protein LOC111809758 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 568.2 bits (1463), Expect = 6.5e-158
Identity = 278/311 (89.39%), Postives = 293/311 (94.21%), Query Frame = 0

Query: 139 KQDETMADETLVEAALRVLNTSDPFEKAELGHSVASRWLDGAISRPYDPSADLAVPDRPA 198
           +Q+ETMADETLVEAALRVLNTSDPFEKAELG  VASRWL+G ISRPYDPS+DLAVPDRPA
Sbjct: 13  RQNETMADETLVEAALRVLNTSDPFEKAELGDKVASRWLNGVISRPYDPSSDLAVPDRPA 72

Query: 199 RLSDVKLVSPSLMPKLGKAGSLQSRQAILHSLVHTESWAIDLSWDIIARFGKQEGMPREF 258
           RLSDVKLVSPSLMPKLGKAGSLQSRQAI+HSLVHTESWAIDLSWDIIARFGKQE MPREF
Sbjct: 73  RLSDVKLVSPSLMPKLGKAGSLQSRQAIVHSLVHTESWAIDLSWDIIARFGKQEEMPREF 132

Query: 259 FTDFVRVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVH 318
           FTDFVRVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVH
Sbjct: 133 FTDFVRVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVH 192

Query: 319 EARGLDVLPTTISRFRKGGDDESADLLEKVVYPEEVTHCAAGVKWFKYLCQRCGEQILDG 378
           EARGLDVLPTTISRFR GG +E+ADLLEKVVYPEEVTHCAAG+KWFKYLCQR G+Q LDG
Sbjct: 193 EARGLDVLPTTISRFRNGGVNETADLLEKVVYPEEVTHCAAGLKWFKYLCQRSGKQTLDG 252

Query: 379 DCSLPVHEDGAEDNA-MEIGNEEIIHKFHAIVRKHFRGPLKPPFNEAARKAAGFGPQWYE 438
           D  L +HE GAEDNA +E+ NEEIIHKFHAIVRKHFRGPLKPPFN  ARKAAGFGP+WYE
Sbjct: 253 DGLLLLHEHGAEDNASLEMENEEIIHKFHAIVRKHFRGPLKPPFNVVARKAAGFGPRWYE 312

Query: 439 PLAFKQDPTLE 449
           PLAFK + TL+
Sbjct: 313 PLAFKPNSTLD 323

BLAST of Lag0029716 vs. NCBI nr
Match: XP_022954428.1 (uncharacterized protein LOC111456685 [Cucurbita moschata])

HSP 1 Score: 565.5 bits (1456), Expect = 4.2e-157
Identity = 276/305 (90.49%), Postives = 289/305 (94.75%), Query Frame = 0

Query: 144 MADETLVEAALRVLNTSDPFEKAELGHSVASRWLDGAISRPYDPSADLAVPDRPARLSDV 203
           MADETLVEAALRVLNTSDPFEKAELG  VASRWL+G ISRPYDPS+DLAVPDRPARLSDV
Sbjct: 1   MADETLVEAALRVLNTSDPFEKAELGDKVASRWLNGVISRPYDPSSDLAVPDRPARLSDV 60

Query: 204 KLVSPSLMPKLGKAGSLQSRQAILHSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFV 263
           KLVSPSLMPKLGKAGSLQSRQAI+HSLVHTESWAIDLSWDIIARFGKQE MPREFFTDFV
Sbjct: 61  KLVSPSLMPKLGKAGSLQSRQAIVHSLVHTESWAIDLSWDIIARFGKQEEMPREFFTDFV 120

Query: 264 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL 323
           RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL
Sbjct: 121 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL 180

Query: 324 DVLPTTISRFRKGGDDESADLLEKVVYPEEVTHCAAGVKWFKYLCQRCGEQILDGDCSLP 383
           DVLPTTISRFR GGD+E+ADLLEKVVYPEEVTHCAAGVKWF+YLCQR G+Q LD D  L 
Sbjct: 181 DVLPTTISRFRNGGDNETADLLEKVVYPEEVTHCAAGVKWFRYLCQRSGKQTLDRDGLLL 240

Query: 384 VHEDGAEDNA-MEIGNEEIIHKFHAIVRKHFRGPLKPPFNEAARKAAGFGPQWYEPLAFK 443
           +H+DGAEDNA +E+ NEEIIHKFHAIVRKHFRGPLKPPFNE ARKAAGFGP+WYEPLAFK
Sbjct: 241 LHQDGAEDNASLEMENEEIIHKFHAIVRKHFRGPLKPPFNEVARKAAGFGPRWYEPLAFK 300

Query: 444 QDPTL 448
            + TL
Sbjct: 301 PNSTL 305

BLAST of Lag0029716 vs. NCBI nr
Match: KAG6602270.1 (hypothetical protein SDJN03_07503, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032955.1 hypothetical protein SDJN02_07006 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 564.7 bits (1454), Expect = 7.2e-157
Identity = 277/306 (90.52%), Postives = 290/306 (94.77%), Query Frame = 0

Query: 144 MADETLVEAALRVLNTSDPFEKAELGHSVASRWLDGAISRPYDPSADLAVPDRPARLSDV 203
           MADETLVEAALRVLNTSDPFEKAELG  VASRWL+G ISRPYDPS+DLAVPDRPARLSDV
Sbjct: 1   MADETLVEAALRVLNTSDPFEKAELGDKVASRWLNGVISRPYDPSSDLAVPDRPARLSDV 60

Query: 204 KLVSPSLMPKLGKAGSLQSRQAILHSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFV 263
           KLVSPSLMPKLGKAGSLQSRQAI+HSLVHTESWAIDLSWDIIARFGKQE MPREFFTDFV
Sbjct: 61  KLVSPSLMPKLGKAGSLQSRQAIVHSLVHTESWAIDLSWDIIARFGKQEEMPREFFTDFV 120

Query: 264 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL 323
           RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL
Sbjct: 121 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL 180

Query: 324 DVLPTTISRFRKGGDDESADLLEKVVYPEEVTHCAAGVKWFKYLCQRCGEQILDGD-CSL 383
           DVLPTTISRFR GGD+E+ADLLEKVVYPEEVTHCAAG+KWFKYLCQR G+Q LDGD   L
Sbjct: 181 DVLPTTISRFRNGGDNETADLLEKVVYPEEVTHCAAGLKWFKYLCQRSGKQTLDGDGLLL 240

Query: 384 PVHEDGAEDNA-MEIGNEEIIHKFHAIVRKHFRGPLKPPFNEAARKAAGFGPQWYEPLAF 443
            +H+DGAEDNA +E+ NEEIIHKFHAIVRKHFRGPLKPPFNE ARKAAGFGP+WYEPLAF
Sbjct: 241 LLHQDGAEDNASLEMENEEIIHKFHAIVRKHFRGPLKPPFNEVARKAAGFGPRWYEPLAF 300

Query: 444 KQDPTL 448
           K + TL
Sbjct: 301 KPNSTL 306

BLAST of Lag0029716 vs. NCBI nr
Match: XP_022990415.1 (uncharacterized protein LOC111487281 [Cucurbita maxima])

HSP 1 Score: 557.8 bits (1436), Expect = 8.8e-155
Identity = 272/305 (89.18%), Postives = 288/305 (94.43%), Query Frame = 0

Query: 144 MADETLVEAALRVLNTSDPFEKAELGHSVASRWLDGAISRPYDPSADLAVPDRPARLSDV 203
           MADETLVEAALRVLNTSDPFEKAELG  VASRWL+G ISRPYDPS+DL+VPDRPARLSDV
Sbjct: 1   MADETLVEAALRVLNTSDPFEKAELGDKVASRWLNGMISRPYDPSSDLSVPDRPARLSDV 60

Query: 204 KLVSPSLMPKLGKAGSLQSRQAILHSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFV 263
           KLVSPSLMPKLGKAGSLQSRQAI+HSLVHTESWAIDLSWDIIARFGKQE MPREFFTDFV
Sbjct: 61  KLVSPSLMPKLGKAGSLQSRQAIVHSLVHTESWAIDLSWDIIARFGKQEEMPREFFTDFV 120

Query: 264 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL 323
           RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATS+DLLARLAIEHCVHEARGL
Sbjct: 121 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSRDLLARLAIEHCVHEARGL 180

Query: 324 DVLPTTISRFRKGGDDESADLLEKVVYPEEVTHCAAGVKWFKYLCQRCGEQILDGDCSLP 383
           DVLPTTISRFR GGD+E+ADLLEKVVYPEEVTHCAAG+KWFKYL QR G+Q LDGD  L 
Sbjct: 181 DVLPTTISRFRNGGDNETADLLEKVVYPEEVTHCAAGLKWFKYLSQRSGKQTLDGDGLLL 240

Query: 384 VHEDGAEDNA-MEIGNEEIIHKFHAIVRKHFRGPLKPPFNEAARKAAGFGPQWYEPLAFK 443
           +H+DGAEDNA +E+ NEEIIHKFHAIVR +FRGPLKPPFNE ARKAAGFGP+WYEPLAFK
Sbjct: 241 LHQDGAEDNASLEMENEEIIHKFHAIVRNYFRGPLKPPFNEVARKAAGFGPRWYEPLAFK 300

Query: 444 QDPTL 448
            + TL
Sbjct: 301 PNSTL 305

BLAST of Lag0029716 vs. NCBI nr
Match: XP_038884770.1 (uncharacterized protein HI_0077 [Benincasa hispida])

HSP 1 Score: 550.4 bits (1417), Expect = 1.4e-152
Identity = 271/304 (89.14%), Postives = 281/304 (92.43%), Query Frame = 0

Query: 144 MADETLVEAALRVLNTSDPFEKAELGHSVASRWLDGAISRPYDPSADLAVPDRPARLSDV 203
           MA ETLVEAALRVLNTSDPFEKAELG  VASRWL+GAIS PYDPSADLAVPDRPARLSDV
Sbjct: 1   MAAETLVEAALRVLNTSDPFEKAELGDHVASRWLNGAISSPYDPSADLAVPDRPARLSDV 60

Query: 204 KLVSPSLMPKLGKAGSLQSRQAILHSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFV 263
           KLVSPSLMPKLGKAGSLQSRQAI+HSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFV
Sbjct: 61  KLVSPSLMPKLGKAGSLQSRQAIVHSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFV 120

Query: 264 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL 323
           RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL
Sbjct: 121 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL 180

Query: 324 DVLPTTISRFRKGGDDESADLLEKVVYPEEVTHCAAGVKWFKYLCQRCGEQILDGDCSLP 383
           DVLPTTISRFR GGD+E+ADLLEKVVYPEEVTHCAAGVKWFKY+CQR G + LD D    
Sbjct: 181 DVLPTTISRFRNGGDNETADLLEKVVYPEEVTHCAAGVKWFKYVCQRSGNRKLDED---- 240

Query: 384 VHEDGAEDNAMEIGNEEIIHKFHAIVRKHFRGPLKPPFNEAARKAAGFGPQWYEPLAFKQ 443
             + GA+DNAME+  EE IHKFH IVRK+FRGPLKPPFNE ARKAAGFGPQWYEPLAFK 
Sbjct: 241 --DAGAKDNAMEMEKEETIHKFHEIVRKYFRGPLKPPFNEVARKAAGFGPQWYEPLAFKT 298

Query: 444 DPTL 448
           DP L
Sbjct: 301 DPIL 298

BLAST of Lag0029716 vs. ExPASy Swiss-Prot
Match: P43935 (Uncharacterized protein HI_0077 OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=HI_0077 PE=4 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 3.2e-22
Identity = 88/287 (30.66%), Postives = 138/287 (48.08%), Query Frame = 0

Query: 150 VEAALRVLNTSDPFEKAELGHSVASRWLDGAISRPYDPSADLAVPDRPARLSDVK-LVSP 209
           VE AL+   T++P EK  L + +    L        +   ++   D  A   +   LV+P
Sbjct: 12  VETALK---TANPQEKCRLVNDLYDNLLPQIQLIKLEDFPEIVPQDNIAAFPEKPLLVAP 71

Query: 210 SLMPKLGKAGSLQSRQAILHSLVHTESWAIDLSWDIIARFGK----QEGMPREFFTDFVR 269
             +PK   A + +   A LH++ H E  AI+L  D   RFG+    + G    F  D++R
Sbjct: 72  KDVPKRSFA-TEEGYAATLHAIAHIEFNAINLGLDAAWRFGRNAQEELGEGLAFVKDWLR 131

Query: 270 VAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGLD 329
           VA++E  HF+L+   LK LG  YG   AH GLW+ A AT+ D+  R+A+   V EARGLD
Sbjct: 132 VAREESTHFSLVNEHLKTLGYQYGDFEAHAGLWEMAQATAHDIWERMALVPRVLEARGLD 191

Query: 330 VLPTTISRFRKGGDDESADLLEKVVYPEEVTHCAAGVKWFKYLCQRCGEQILDGDCSLPV 389
             P    +  +  D  + ++L+ ++  +E+ H   G  W+  L ++ G   LD       
Sbjct: 192 ATPVLQEKIAQRKDFAAVNILD-IILRDEIGHVYIGNHWYHALSKKRG---LD------- 251

Query: 390 HEDGAEDNAMEIGNEEIIHKFHAIVRKHFRGPLKPPFNEAARKAAGF 432
                   AM+    E++HK+  ++   F+G +    N  AR  AGF
Sbjct: 252 --------AMKCFT-ELLHKYRIVI---FKGVI----NTDARIQAGF 267

BLAST of Lag0029716 vs. ExPASy TrEMBL
Match: A0A6J1GR27 (uncharacterized protein LOC111456685 OS=Cucurbita moschata OX=3662 GN=LOC111456685 PE=4 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 2.1e-157
Identity = 276/305 (90.49%), Postives = 289/305 (94.75%), Query Frame = 0

Query: 144 MADETLVEAALRVLNTSDPFEKAELGHSVASRWLDGAISRPYDPSADLAVPDRPARLSDV 203
           MADETLVEAALRVLNTSDPFEKAELG  VASRWL+G ISRPYDPS+DLAVPDRPARLSDV
Sbjct: 1   MADETLVEAALRVLNTSDPFEKAELGDKVASRWLNGVISRPYDPSSDLAVPDRPARLSDV 60

Query: 204 KLVSPSLMPKLGKAGSLQSRQAILHSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFV 263
           KLVSPSLMPKLGKAGSLQSRQAI+HSLVHTESWAIDLSWDIIARFGKQE MPREFFTDFV
Sbjct: 61  KLVSPSLMPKLGKAGSLQSRQAIVHSLVHTESWAIDLSWDIIARFGKQEEMPREFFTDFV 120

Query: 264 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL 323
           RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL
Sbjct: 121 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL 180

Query: 324 DVLPTTISRFRKGGDDESADLLEKVVYPEEVTHCAAGVKWFKYLCQRCGEQILDGDCSLP 383
           DVLPTTISRFR GGD+E+ADLLEKVVYPEEVTHCAAGVKWF+YLCQR G+Q LD D  L 
Sbjct: 181 DVLPTTISRFRNGGDNETADLLEKVVYPEEVTHCAAGVKWFRYLCQRSGKQTLDRDGLLL 240

Query: 384 VHEDGAEDNA-MEIGNEEIIHKFHAIVRKHFRGPLKPPFNEAARKAAGFGPQWYEPLAFK 443
           +H+DGAEDNA +E+ NEEIIHKFHAIVRKHFRGPLKPPFNE ARKAAGFGP+WYEPLAFK
Sbjct: 241 LHQDGAEDNASLEMENEEIIHKFHAIVRKHFRGPLKPPFNEVARKAAGFGPRWYEPLAFK 300

Query: 444 QDPTL 448
            + TL
Sbjct: 301 PNSTL 305

BLAST of Lag0029716 vs. ExPASy TrEMBL
Match: A0A6J1JQ17 (uncharacterized protein LOC111487281 OS=Cucurbita maxima OX=3661 GN=LOC111487281 PE=4 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 4.3e-155
Identity = 272/305 (89.18%), Postives = 288/305 (94.43%), Query Frame = 0

Query: 144 MADETLVEAALRVLNTSDPFEKAELGHSVASRWLDGAISRPYDPSADLAVPDRPARLSDV 203
           MADETLVEAALRVLNTSDPFEKAELG  VASRWL+G ISRPYDPS+DL+VPDRPARLSDV
Sbjct: 1   MADETLVEAALRVLNTSDPFEKAELGDKVASRWLNGMISRPYDPSSDLSVPDRPARLSDV 60

Query: 204 KLVSPSLMPKLGKAGSLQSRQAILHSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFV 263
           KLVSPSLMPKLGKAGSLQSRQAI+HSLVHTESWAIDLSWDIIARFGKQE MPREFFTDFV
Sbjct: 61  KLVSPSLMPKLGKAGSLQSRQAIVHSLVHTESWAIDLSWDIIARFGKQEEMPREFFTDFV 120

Query: 264 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL 323
           RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATS+DLLARLAIEHCVHEARGL
Sbjct: 121 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSRDLLARLAIEHCVHEARGL 180

Query: 324 DVLPTTISRFRKGGDDESADLLEKVVYPEEVTHCAAGVKWFKYLCQRCGEQILDGDCSLP 383
           DVLPTTISRFR GGD+E+ADLLEKVVYPEEVTHCAAG+KWFKYL QR G+Q LDGD  L 
Sbjct: 181 DVLPTTISRFRNGGDNETADLLEKVVYPEEVTHCAAGLKWFKYLSQRSGKQTLDGDGLLL 240

Query: 384 VHEDGAEDNA-MEIGNEEIIHKFHAIVRKHFRGPLKPPFNEAARKAAGFGPQWYEPLAFK 443
           +H+DGAEDNA +E+ NEEIIHKFHAIVR +FRGPLKPPFNE ARKAAGFGP+WYEPLAFK
Sbjct: 241 LHQDGAEDNASLEMENEEIIHKFHAIVRNYFRGPLKPPFNEVARKAAGFGPRWYEPLAFK 300

Query: 444 QDPTL 448
            + TL
Sbjct: 301 PNSTL 305

BLAST of Lag0029716 vs. ExPASy TrEMBL
Match: A0A0A0LMC3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G385090 PE=4 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 9.9e-152
Identity = 268/303 (88.45%), Postives = 281/303 (92.74%), Query Frame = 0

Query: 144 MADETLVEAALRVLNTSDPFEKAELGHSVASRWLDGAISRPYDPSADLAVPDRPARLSDV 203
           MADETLVEAALRVLNTSDPFEKAELG +VASRWL+GAIS PYDPSADL VPDRPARLS+V
Sbjct: 1   MADETLVEAALRVLNTSDPFEKAELGDNVASRWLNGAISNPYDPSADLPVPDRPARLSNV 60

Query: 204 KLVSPSLMPKLGKAGSLQSRQAILHSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFV 263
           KLVSPSLMPKLGKAGSLQSRQAI+HSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFV
Sbjct: 61  KLVSPSLMPKLGKAGSLQSRQAIVHSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFV 120

Query: 264 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL 323
           RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL
Sbjct: 121 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL 180

Query: 324 DVLPTTISRFRKGGDDESADLLEKVVYPEEVTHCAAGVKWFKYLCQRCGEQILDGDCSLP 383
           DVLPTTI RFR GGD+E+ADLLEKVVYPEEVTHCAAGVKWFKYLCQR  ++ LD D    
Sbjct: 181 DVLPTTIYRFRNGGDNETADLLEKVVYPEEVTHCAAGVKWFKYLCQRSIDRKLDED---- 240

Query: 384 VHEDGAEDNAMEIGNEEIIHKFHAIVRKHFRGPLKPPFNEAARKAAGFGPQWYEPLAFKQ 443
             +DGAE NAME+  EE I+KFH +VRK+FRGPLKPPFNE ARKAAGFGPQWYEPLAFK 
Sbjct: 241 --DDGAESNAMEMEKEETINKFHEVVRKYFRGPLKPPFNEVARKAAGFGPQWYEPLAFKT 297

Query: 444 DPT 447
           DPT
Sbjct: 301 DPT 297

BLAST of Lag0029716 vs. ExPASy TrEMBL
Match: A0A1S3C3M9 (uncharacterized protein HI_0077 OS=Cucumis melo OX=3656 GN=LOC103496484 PE=4 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 2.7e-149
Identity = 266/303 (87.79%), Postives = 278/303 (91.75%), Query Frame = 0

Query: 144 MADETLVEAALRVLNTSDPFEKAELGHSVASRWLDGAISRPYDPSADLAVPDRPARLSDV 203
           MADETLVEAALRVLNTSDPFEKA LG +VASRWL+GAIS  YDPSADL VPDRPARLS+V
Sbjct: 1   MADETLVEAALRVLNTSDPFEKAGLGDNVASRWLNGAISSLYDPSADLPVPDRPARLSNV 60

Query: 204 KLVSPSLMPKLGKAGSLQSRQAILHSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFV 263
           KLVSPSLMPKLGKAGSLQSRQAI+HSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFV
Sbjct: 61  KLVSPSLMPKLGKAGSLQSRQAIVHSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFV 120

Query: 264 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL 323
           RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL
Sbjct: 121 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL 180

Query: 324 DVLPTTISRFRKGGDDESADLLEKVVYPEEVTHCAAGVKWFKYLCQRCGEQILDGDCSLP 383
           DVLPTTI RFR GGD+E+ADLLEKVVYPEEVTHCAAGVKWFKYLCQR  ++ LD D    
Sbjct: 181 DVLPTTIYRFRNGGDNETADLLEKVVYPEEVTHCAAGVKWFKYLCQRSMDRKLDED---- 240

Query: 384 VHEDGAEDNAMEIGNEEIIHKFHAIVRKHFRGPLKPPFNEAARKAAGFGPQWYEPLAFKQ 443
             +DGAEDNAME+  EE I KFH +VRK+FRGPLKPPFNE ARKAAGFGPQWYEPLAFK 
Sbjct: 241 --DDGAEDNAMEMEKEETIKKFHEVVRKYFRGPLKPPFNEVARKAAGFGPQWYEPLAFKT 297

Query: 444 DPT 447
           D T
Sbjct: 301 DST 297

BLAST of Lag0029716 vs. ExPASy TrEMBL
Match: A0A6J1BXS8 (uncharacterized protein LOC111006681 OS=Momordica charantia OX=3673 GN=LOC111006681 PE=4 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 2.6e-144
Identity = 257/301 (85.38%), Postives = 272/301 (90.37%), Query Frame = 0

Query: 144 MADETLVEAALRVLNTSDPFEKAELGHSVASRWLDGAISRPYDPSADLAVPDRPARLSDV 203
           M DETLVEAALRVLNTSDP+EKAELG SVASRWL GAIS PYDPS DL VPDRPARLSDV
Sbjct: 1   MGDETLVEAALRVLNTSDPYEKAELGDSVASRWLSGAISLPYDPSVDLQVPDRPARLSDV 60

Query: 204 KLVSPSLMPKLGKAGSLQSRQAILHSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFV 263
           KLVSPSLMPKLG+AGSL SRQAI+HSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFV
Sbjct: 61  KLVSPSLMPKLGRAGSLHSRQAIVHSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFV 120

Query: 264 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGL 323
           RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSA ATSKDLLARLAIEHCVHEARGL
Sbjct: 121 RVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDSATATSKDLLARLAIEHCVHEARGL 180

Query: 324 DVLPTTISRFRKGGDDESADLLEKVVYPEEVTHCAAGVKWFKYLCQRCGEQILDGDCSLP 383
           DVLPTTI RFR GGDDE+ADLLEKVVYPEEVTHC AGVKWFKYLC+R G+ +LD      
Sbjct: 181 DVLPTTICRFRNGGDDETADLLEKVVYPEEVTHCGAGVKWFKYLCERSGKLMLD------ 240

Query: 384 VHEDGAEDNAMEIGNEEIIHKFHAIVRKHFRGPLKPPFNEAARKAAGFGPQWYEPLAFKQ 443
             ED  E NA+++ +E+II+KFH IVR +FRGPLKPPFNEAARKAAGFGP+WYEPLA K 
Sbjct: 241 -EED--EANAVKMEDEDIINKFHEIVRNYFRGPLKPPFNEAARKAAGFGPRWYEPLALKT 292

Query: 444 D 445
           D
Sbjct: 301 D 292

BLAST of Lag0029716 vs. TAIR 10
Match: AT5G04520.1 (Protein of unknown function DUF455 )

HSP 1 Score: 474.6 bits (1220), Expect = 9.2e-134
Identity = 230/297 (77.44%), Postives = 256/297 (86.20%), Query Frame = 0

Query: 147 ETLVEAALRVLNTSDPFEKAELGHSVASRWLDGAISRPYDPSADLAVPDRPARLSDVKLV 206
           ETL+E+A+R+LNTSDP EKA LG S+A +WL GAI+ PYDP+ D  VPDRPARL  VKLV
Sbjct: 4   ETLIESAIRILNTSDPHEKARLGDSIAVKWLQGAIAEPYDPTVDFPVPDRPARL-PVKLV 63

Query: 207 SPSLMPKLGKAGSLQSRQAILHSLVHTESWAIDLSWDIIARFGKQEGMPREFFTDFVRVA 266
           SPSLMPKLG+AGSLQSRQAI+HSL HTESWAIDLSWDIIARFGKQE MPR+FFTDFVRVA
Sbjct: 64  SPSLMPKLGRAGSLQSRQAIVHSLAHTESWAIDLSWDIIARFGKQEKMPRDFFTDFVRVA 123

Query: 267 QDEGRHFTLLAARLKELGSFYGALPAHDGLWDSAIATSKDLLARLAIEHCVHEARGLDVL 326
           QDEGRHFTLLAARL+E+GS YGALPAHDGLWDSA ATS DLLARLAIEHCVHEARGLDVL
Sbjct: 124 QDEGRHFTLLAARLEEIGSSYGALPAHDGLWDSATATSHDLLARLAIEHCVHEARGLDVL 183

Query: 327 PTTISRFRKGGDDESADLLEKVVYPEEVTHCAAGVKWFKYLCQRCGEQILDGDCSLPVHE 386
           PTTISRFR GGD+E+ADLLEKVVYPEE+THCAAGVKWFKYLC+R        D    +  
Sbjct: 184 PTTISRFRNGGDNETADLLEKVVYPEEITHCAAGVKWFKYLCER------SKDPEFTISS 243

Query: 387 DGAEDNAMEIGNEEIIHKFHAIVRKHFRGPLKPPFNEAARKAAGFGPQWYEPLAFKQ 444
             ++D+     NEEII+KFH++VR+HFRGPLKPPFN  ARKAAGFGPQWYEPLA K+
Sbjct: 244 KESDDS-----NEEIINKFHSVVREHFRGPLKPPFNAEARKAAGFGPQWYEPLAVKE 288

BLAST of Lag0029716 vs. TAIR 10
Match: AT1G06240.1 (Protein of unknown function DUF455 )

HSP 1 Score: 156.4 bits (394), Expect = 5.5e-38
Identity = 107/319 (33.54%), Postives = 149/319 (46.71%), Query Frame = 0

Query: 130 GRRGGSLRTKQDETMAD--------ETLVEAALRVLNTSDPFEKAELGHSVASRWLDGAI 189
           G  G  L +  D   AD         +L +    VL+TSDP  K+ + H   SRW     
Sbjct: 55  GPTGPLLPSSSDSISADFYGLVSAASSLADLGALVLSTSDPLSKSHISHLAFSRW----- 114

Query: 190 SRPYDPSADLA-VPDRPARLSDVKLVSPSLMPKLGKAGSLQSRQAILHSLVHTESWAIDL 249
            R   P   ++ +P  PAR     LV+ + +P   K  +L     +LH+L H E  AIDL
Sbjct: 115 RRENLPVGSISHLPSSPARPPKPLLVATNQVPN-PKDSNLPLNAHMLHNLAHVELNAIDL 174

Query: 250 SWDIIARFGK-QEGMPREFFTDFVRVAQDEGRHFTLLAARLKELGSFYGALPAHDGLWDS 309
           +WD +ARF    + +   FF DF  VA DE RHF   + RL ELG  YG +PA++ L   
Sbjct: 175 AWDTVARFSPFFDLLGHNFFDDFAHVADDESRHFLWCSQRLAELGFKYGDIPANNLLMRE 234

Query: 310 AIATSKDLLARLAIEHCVHEARGLDVLPTTISRFRKGGDDESADLLEKVVYPEEVTHCAA 369
              TS ++ ARLA    V EARGLD  P  + R    GD+ ++ ++ K+   EEV H A 
Sbjct: 235 CEKTSNNVAARLACIPLVQEARGLDAGPRLVKRLTGFGDNRTSKIVAKIA-EEEVAHVAV 294

Query: 370 GVKWFKYLCQRCGEQILDGDCSLPVHEDGAEDNAMEIGNEEIIHKFHAIVRKHFRGPLKP 429
           GV WF  +CQ+            P  +D  ++  +E                     L+ 
Sbjct: 295 GVDWFLSVCQKMNR------APSPTFKDLIKEYGVE---------------------LRG 339

Query: 430 PFNEAARKAAGFGPQWYEP 439
           PFN +AR+ AG    WY+P
Sbjct: 355 PFNHSAREVAGIPRDWYDP 339