Lag0029675 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0029675
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionvesicle-associated membrane protein 727
Locationchr8: 41085800 .. 41088465 (+)
RNA-Seq ExpressionLag0029675
SyntenyLag0029675
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTCAGAAAGGCCTAATATATAGCTTTGTTGCTAAAGGAACTGTTGTTTTAGCAGAACACACATCATTCTCAGGCAATTTTAGTACCATTGCCGTTCAATGCTTACAGAGACTGCCTTCTAATGGCACCAAATGCACATACTCATGTGATGGGCATACATTCAACTTTCTTCTTGACAGTGGATTTGGTATTGTGTTTTCTCAAGTTTGTTATATACTTGTTTTAACTTCACTAGCACTTGTTGCTGAAGATGTTGGATTGATTTGCAGTTTTTCTGGCTGTTGCGGATGAATCAGTTGGAAGGAATATGCCATTTGTATTTCTTGAGCGTGTGAAAGATGACTTTAAACAACGTTATGGTTCAAGTATCAAGGATGAGAACCCTCACCCACTTGCAGATGATGAGGACGATGATGATTTATTTTTAGACCGATTCAGTGTTGCTTACACCCTTGACAGAGAATTTGGGTATGCCCATGTTTATAAACAACATTGGAGTTCTATGTCATTCATTTTATTTAACGACCTCTAATTTTCATGATAGGCCAAGGCTTAAGGAGCACATGCAGTATTGCATGAGCCATCCAGAAGAAATGAGTAAACTTTCCAAGCTGAAGGCTCAAATAACAGAAGTGAAAGGCATTATGATGGACAACATTGAAAAGGTTAGACTTCTTGTTTTATGAAATCATGCAATTTATGCTCAAGATTTTACATTGAGATTTTGACAGAGTCTATATGTTCTTTTTCACTGTTGTGCATAATGTATAAACATGGAGAAAGAGATTAAAGCATTTTAGAAAATCTCACTTTGCACCCGCTAATTCTTCCTCCTTTTTCCATCTCATCTTGGCAAATATACTAAATCACATTGAACATGTACCTAGGCATATCCCTGTTACATGCGGCATCCTGGTAGGTAGATCAGCCAAGTAGTATATTATCACTGAATACTGAACAAATTTTTGCTCTCTCTAAGCAATGCAGTCAATACTTTAAGATTTCAGTAATTATGTTGCAAGGAAATTGATATTACACAAGTGTGTTGCTTGCTTGTATTCAAAGAACCAGATTTTCAGCTGGACCAGTTGAAGAATAATTGTTTACAAAGAATTTGGTAGTGGTCAGTTTTGGAAAAAGTGGAATGGAATGGGTCAAATATCACACACAGATTCTTTTCCTTTTTCTGCCCTTCCTTGCTTCTGAATACAGCCATAGGAAATGATTGTGGTGATTTTGAGCAATAAAGCATCACTTTAGCCTTTTGTCTTAGACACAATAAAGTGCCAGTTTGATAGGATTTTACATTATATTATTACTGATTTCCTTACTTGAACTTACAAGTTACTGAGTACATACAAAAGATCAACTGTTTGATATCGTATATGCTGTTTAATTTACATGTATATTATTGAAGCCATATGTCGAACCTTGGTGAGAAATTTGGTGGCATATCCTTGAGTGGGTAATGGAAATTTGTTGGAATGGACTATCTATCCACTTTTTGACAATAAAATCAAGGTAGTAAAATATGATTTCTTTTTATTACTTACATGGGGATTGAAAAAATAATAATAATAAATATAAAAAACAATCTGCTTCTAGCTTTCCTTCTTGATGCTCTCTGTGGAAGCTAAAACTTTGAGATGGAACTCCTGCGAAGCTAAGGAATGCTAGACCTTTTTAAGACGAGACTAGGGATTTGGATGGGATTGTTGACAATTATTTTAAAAAAATATTCGCTTCGAAGCTCTCTTGATAATAGGTTCTTTTGTAACTACCCTCTCACTGTGAGTGTGATTGGGTTTTTATTAGTCCTTTTGTTCTTGGGTTGGGTAGTGCCTCATCATTCCTTGTATACTCTTCCACATTTTAAATGGTCTGGAATTGGTGAATCAGTACTTTGGACTTCTGATATTATTATCAATTTTTTGGGTGGTGATCTGGTCTTTTCAGTTTTTAACCCATGGACATGTTGTTTTTCATTTTTCATTGTTCATTTTTTATTTTTGATTGAGTAAAGGCAATCGTTGAATCACCTATGCCCATTGAAGTCACCGAATTGAGATCTTGCTAGGGTTTGCCAAGGGAAAAAAAATGCAAACCATACTTTTTGTACACTTTGAGTATGACTACTCCATTTACTTATATTTTTTTTCCTGTAATAAAGAGGAGGAGAGAGATGCTTTGAAGTTACTTGTTCAAAGAAACTGAAAATGAGGAGTTTTCTTCCAGTTAGCTGCACTAGCCACACTAGCCACTAAGTTGCGTGTACTTAAGAAGAGGGAGACTCGGGGCTAGGAAATTTAAAACCTATCCTTATCAAATGTTCTACACAGATATGATATCAATTTCCTTTTCCTCGTGTAGGTTTTAGATCGTGGAGAACGTATCGAGCTTCTGGTAGACAAGACAGAAAACCTACAATTTCAGGTAACATCATATAATCTGATGACAGTTGAAAGACAAAAACTCGTCTATCTAACCGTTTTTAAGTTGGAATCTTTGTTTAAATTTAGGCCGATAACTTTCACCGACAAGGAAGGCAACTTCGGCGAAAAATGTGGCTGCAGAGTTTACAAATGAAGCTGATGGTTGGGGGAGGAATATTGGTCTTGATCATCATACTTTGGTTTATTGTATGTGGTGGTTTCAAATGTTGA

mRNA sequence

ATGAGTCAGAAAGGCCTAATATATAGCTTTGTTGCTAAAGGAACTGTTGTTTTAGCAGAACACACATCATTCTCAGGCAATTTTAGTACCATTGCCGTTCAATGCTTACAGAGACTGCCTTCTAATGGCACCAAATGCACATACTCATGTGATGGGCATACATTCAACTTTCTTCTTGACAGTGGATTTGTTTTTCTGGCTGTTGCGGATGAATCAGTTGGAAGGAATATGCCATTTGTATTTCTTGAGCGTGTGAAAGATGACTTTAAACAACGTTATGGTTCAAGTATCAAGGATGAGAACCCTCACCCACTTGCAGATGATGAGGACGATGATGATTTATTTTTAGACCGATTCAGTGTTGCTTACACCCTTGACAGAGAATTTGGGCCAAGGCTTAAGGAGCACATGCAGTATTGCATGAGCCATCCAGAAGAAATGAGTAAACTTTCCAAGCTGAAGGCTCAAATAACAGAAGTGAAAGGCATTATGATGGACAACATTGAAAAGGTTTTAGATCGTGGAGAACGTATCGAGCTTCTGGTAGACAAGACAGAAAACCTACAATTTCAGGCCGATAACTTTCACCGACAAGGAAGGCAACTTCGGCGAAAAATGTGGCTGCAGAGTTTACAAATGAAGCTGATGGTTGGGGGAGGAATATTGGTCTTGATCATCATACTTTGGTTTATTGTATGTGGTGGTTTCAAATGTTGA

Coding sequence (CDS)

ATGAGTCAGAAAGGCCTAATATATAGCTTTGTTGCTAAAGGAACTGTTGTTTTAGCAGAACACACATCATTCTCAGGCAATTTTAGTACCATTGCCGTTCAATGCTTACAGAGACTGCCTTCTAATGGCACCAAATGCACATACTCATGTGATGGGCATACATTCAACTTTCTTCTTGACAGTGGATTTGTTTTTCTGGCTGTTGCGGATGAATCAGTTGGAAGGAATATGCCATTTGTATTTCTTGAGCGTGTGAAAGATGACTTTAAACAACGTTATGGTTCAAGTATCAAGGATGAGAACCCTCACCCACTTGCAGATGATGAGGACGATGATGATTTATTTTTAGACCGATTCAGTGTTGCTTACACCCTTGACAGAGAATTTGGGCCAAGGCTTAAGGAGCACATGCAGTATTGCATGAGCCATCCAGAAGAAATGAGTAAACTTTCCAAGCTGAAGGCTCAAATAACAGAAGTGAAAGGCATTATGATGGACAACATTGAAAAGGTTTTAGATCGTGGAGAACGTATCGAGCTTCTGGTAGACAAGACAGAAAACCTACAATTTCAGGCCGATAACTTTCACCGACAAGGAAGGCAACTTCGGCGAAAAATGTGGCTGCAGAGTTTACAAATGAAGCTGATGGTTGGGGGAGGAATATTGGTCTTGATCATCATACTTTGGTTTATTGTATGTGGTGGTTTCAAATGTTGA

Protein sequence

MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLDSGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFSVAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIELLVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC
Homology
BLAST of Lag0029675 vs. NCBI nr
Match: XP_038884037.1 (vesicle-associated membrane protein 727 [Benincasa hispida])

HSP 1 Score: 470.3 bits (1209), Expect = 9.8e-129
Identity = 234/238 (98.32%), Postives = 237/238 (99.58%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSN +KCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPRLKEHMQYCM+HPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGIL+LIIILWFIVCGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC 238

BLAST of Lag0029675 vs. NCBI nr
Match: XP_008456603.1 (PREDICTED: vesicle-associated membrane protein 727 [Cucumis melo] >TYK11767.1 vesicle-associated membrane protein 727 [Cucumis melo var. makuwa])

HSP 1 Score: 470.3 bits (1209), Expect = 9.8e-129
Identity = 235/238 (98.74%), Postives = 236/238 (99.16%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSN +KCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVL IILWFIVCGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLFIILWFIVCGGFKC 238

BLAST of Lag0029675 vs. NCBI nr
Match: XP_022939856.1 (vesicle-associated membrane protein 727-like [Cucurbita moschata] >XP_022939857.1 vesicle-associated membrane protein 727-like [Cucurbita moschata] >KAG7016346.1 Vesicle-associated membrane protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 466.1 bits (1198), Expect = 1.8e-127
Identity = 231/238 (97.06%), Postives = 237/238 (99.58%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQ+LPSN +KCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDED+DDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDEDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPR+KEHMQYCM+HPEEM+KLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRIKEHMQYCMNHPEEMNKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 238

BLAST of Lag0029675 vs. NCBI nr
Match: XP_004140905.1 (vesicle-associated membrane protein 727 [Cucumis sativus] >KGN46041.1 hypothetical protein Csa_005451 [Cucumis sativus])

HSP 1 Score: 466.1 bits (1198), Expect = 1.8e-127
Identity = 231/238 (97.06%), Postives = 236/238 (99.16%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKG+VVLAEHTSFSGNFSTIAVQCLQRLPSN +KCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGSVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRNMPFVFL+RVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGP+LKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPKLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVL +ILWFIVCGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLFVILWFIVCGGFKC 238

BLAST of Lag0029675 vs. NCBI nr
Match: XP_023551597.1 (vesicle-associated membrane protein 727-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 464.9 bits (1195), Expect = 4.1e-127
Identity = 230/238 (96.64%), Postives = 237/238 (99.58%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQ+LPSN +KCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKD+NPHPLADDED+DDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDDNPHPLADDEDEDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPR+KEHMQYCM+HPEEM+KLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRIKEHMQYCMNHPEEMNKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 238

BLAST of Lag0029675 vs. ExPASy Swiss-Prot
Match: Q9M376 (Vesicle-associated membrane protein 727 OS=Arabidopsis thaliana OX=3702 GN=VAMP727 PE=1 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 5.6e-111
Identity = 197/238 (82.77%), Postives = 219/238 (92.02%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHT +SGNFSTIAVQCLQ+LP+N +K TYSCDGHTFNFL+D
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTPYSGNFSTIAVQCLQKLPTNSSKYTYSCDGHTFNFLVD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GFVFL VADES GR++PFVFLERVK+DFK+RY +SIK++  HPLAD+++DDDLF DRFS
Sbjct: 61  NGFVFLVVADESTGRSVPFVFLERVKEDFKKRYEASIKNDERHPLADEDEDDDLFGDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAY LDREFGP LKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGE+IEL
Sbjct: 121 VAYNLDREFGPILKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENLQFQAD+F RQGRQLRRKMWLQSLQMKLMV G +   I+I+W + CGGFKC
Sbjct: 181 LVDKTENLQFQADSFQRQGRQLRRKMWLQSLQMKLMVAGAVFSFILIVWVVACGGFKC 238

BLAST of Lag0029675 vs. ExPASy Swiss-Prot
Match: O48850 (Vesicle-associated membrane protein 725 OS=Arabidopsis thaliana OX=3702 GN=VAMP725 PE=2 SV=2)

HSP 1 Score: 276.9 bits (707), Expect = 2.1e-73
Identity = 136/238 (57.14%), Postives = 179/238 (75.21%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           M Q+ LIYSFVA+GTV+L E+T F GNF+ +A QCLQ+LPS+  K TY+CDGHTFN+L++
Sbjct: 66  MGQQNLIYSFVARGTVILVEYTEFKGNFTAVAAQCLQKLPSSNNKFTYNCDGHTFNYLVE 125

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  VA ESVGR +P  FLERVK+DF +RYG                         +
Sbjct: 126 NGFTYCVVAVESVGRQIPMAFLERVKEDFNKRYGGGKATT-------------------A 185

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L+REFG +LKEHMQYC+ HP+E+SKL+K+KAQ+TEVKG+MM+NIEKVLDRGE+IEL
Sbjct: 186 QANSLNREFGSKLKEHMQYCVDHPDEISKLAKVKAQVTEVKGVMMENIEKVLDRGEKIEL 245

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENL+ QA +F  QG ++RRKMW +++++KL+V G I+ LI+I+   VCGGFKC
Sbjct: 246 LVDKTENLRSQAQDFRTQGTKIRRKMWFENMKIKLIVLGIIITLILIIILSVCGGFKC 284

BLAST of Lag0029675 vs. ExPASy Swiss-Prot
Match: Q9MAS5 (Putative vesicle-associated membrane protein 726 OS=Arabidopsis thaliana OX=3702 GN=VAMP726 PE=2 SV=2)

HSP 1 Score: 271.9 bits (694), Expect = 6.7e-72
Identity = 134/238 (56.30%), Postives = 176/238 (73.95%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           M Q+ LIYSFVA+GTV+LAE+T F GNF+++A QCLQ+LPS+  K TY+CDGHTFN+L D
Sbjct: 1   MGQQSLIYSFVARGTVILAEYTEFKGNFTSVAAQCLQKLPSSNNKFTYNCDGHTFNYLAD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  V  ES GR +P  FLERVK+DF +RYG                         +
Sbjct: 61  NGFTYCVVVIESAGRQIPMAFLERVKEDFNKRYGGGKAST-------------------A 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L++EFG +LKEHMQYC  HPEE+SKLSK+KAQ+TEVKG+MM+NIEKVLDRGE+IEL
Sbjct: 121 KANSLNKEFGSKLKEHMQYCADHPEEISKLSKVKAQVTEVKGVMMENIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENL+ QA +F  QG +++RK+W +++++KL+V G I+ LI+I+   VC GFKC
Sbjct: 181 LVDKTENLRSQAQDFRTQGTKMKRKLWFENMKIKLIVFGIIVALILIIILSVCHGFKC 219

BLAST of Lag0029675 vs. ExPASy Swiss-Prot
Match: P47192 (Vesicle-associated membrane protein 722 OS=Arabidopsis thaliana OX=3702 GN=VAMP722 PE=2 SV=2)

HSP 1 Score: 271.2 bits (692), Expect = 1.1e-71
Identity = 133/238 (55.88%), Postives = 177/238 (74.37%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           M+Q+ LIYSFVA+GTV+L E T F GNF++IA QCLQ+LPS+  K TY+CDGHTFN+L++
Sbjct: 1   MAQQSLIYSFVARGTVILVEFTDFKGNFTSIAAQCLQKLPSSNNKFTYNCDGHTFNYLVE 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  VA +S GR +P  FLERVK+DF +RYG                         +
Sbjct: 61  NGFTYCVVAVDSAGRQIPMAFLERVKEDFNKRYGGG-------------------KAATA 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L++EFG +LKEHMQYCM HP+E+SKL+K+KAQ++EVKG+MM+NIEKVLDRGE+IEL
Sbjct: 121 QANSLNKEFGSKLKEHMQYCMDHPDEISKLAKVKAQVSEVKGVMMENIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENL+ QA +F  QG Q+RRKMW Q++++KL+V   I+ LI+I+   +CGGF C
Sbjct: 181 LVDKTENLRSQAQDFRTQGTQMRRKMWFQNMKIKLIVLAIIIALILIIILSICGGFNC 219

BLAST of Lag0029675 vs. ExPASy Swiss-Prot
Match: Q9ZTW3 (Vesicle-associated membrane protein 721 OS=Arabidopsis thaliana OX=3702 GN=VAMP721 PE=2 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 7.4e-71
Identity = 134/238 (56.30%), Postives = 176/238 (73.95%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           M+Q+ LIYSFVA+GTV+L E T F GNF++IA QCLQ+LPS+  K TY+CDGHTFN+L++
Sbjct: 1   MAQQSLIYSFVARGTVILVEFTDFKGNFTSIAAQCLQKLPSSNNKFTYNCDGHTFNYLVE 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
            GF +  VA +S GR +P  FLERVK+DF +RYG                         +
Sbjct: 61  DGFTYCVVAVDSAGRQIPMSFLERVKEDFNKRYGGG-------------------KAATA 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L++EFG +LKEHMQYCM HP+E+SKL+K+KAQ++EVKG+MM+NIEKVLDRGE+IEL
Sbjct: 121 QANSLNKEFGSKLKEHMQYCMDHPDEISKLAKVKAQVSEVKGVMMENIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENL+ QA +F   G Q+RRKMWLQ++++KL+V   I+ LI+I+   VC GFKC
Sbjct: 181 LVDKTENLRSQAQDFRTTGTQMRRKMWLQNMKIKLIVLAIIIALILIIVLSVCHGFKC 219

BLAST of Lag0029675 vs. ExPASy TrEMBL
Match: A0A5D3CIL2 (Vesicle-associated membrane protein 727 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold304G00880 PE=3 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 4.7e-129
Identity = 235/238 (98.74%), Postives = 236/238 (99.16%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSN +KCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVL IILWFIVCGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLFIILWFIVCGGFKC 238

BLAST of Lag0029675 vs. ExPASy TrEMBL
Match: A0A1S3C3Q2 (vesicle-associated membrane protein 727 OS=Cucumis melo OX=3656 GN=LOC103496513 PE=3 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 4.7e-129
Identity = 235/238 (98.74%), Postives = 236/238 (99.16%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSN +KCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVL IILWFIVCGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLFIILWFIVCGGFKC 238

BLAST of Lag0029675 vs. ExPASy TrEMBL
Match: A0A6J1FGZ7 (vesicle-associated membrane protein 727-like OS=Cucurbita moschata OX=3662 GN=LOC111445601 PE=3 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 8.9e-128
Identity = 231/238 (97.06%), Postives = 237/238 (99.58%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQ+LPSN +KCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDED+DDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDEDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPR+KEHMQYCM+HPEEM+KLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRIKEHMQYCMNHPEEMNKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 238

BLAST of Lag0029675 vs. ExPASy TrEMBL
Match: A0A0A0KE69 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G045200 PE=3 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 8.9e-128
Identity = 231/238 (97.06%), Postives = 236/238 (99.16%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKG+VVLAEHTSFSGNFSTIAVQCLQRLPSN +KCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGSVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRNMPFVFL+RVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGP+LKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPKLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVL +ILWFIVCGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLFVILWFIVCGGFKC 238

BLAST of Lag0029675 vs. ExPASy TrEMBL
Match: A0A6J1JTB2 (vesicle-associated membrane protein 727-like OS=Cucurbita maxima OX=3661 GN=LOC111487304 PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.0e-127
Identity = 230/238 (96.64%), Postives = 237/238 (99.58%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNG+KCTYSCDG+TFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGSKCTYSCDGYTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIK+E+PHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKEEDPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPRLKEHMQYCM+HPEEMSKLSKLKAQITEVKG+MMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGVMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVL IILWF+VCGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLFIILWFVVCGGFKC 238

BLAST of Lag0029675 vs. TAIR 10
Match: AT3G54300.1 (vesicle-associated membrane protein 727 )

HSP 1 Score: 401.7 bits (1031), Expect = 4.0e-112
Identity = 197/238 (82.77%), Postives = 219/238 (92.02%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHT +SGNFSTIAVQCLQ+LP+N +K TYSCDGHTFNFL+D
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTPYSGNFSTIAVQCLQKLPTNSSKYTYSCDGHTFNFLVD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GFVFL VADES GR++PFVFLERVK+DFK+RY +SIK++  HPLAD+++DDDLF DRFS
Sbjct: 61  NGFVFLVVADESTGRSVPFVFLERVKEDFKKRYEASIKNDERHPLADEDEDDDLFGDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAY LDREFGP LKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGE+IEL
Sbjct: 121 VAYNLDREFGPILKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENLQFQAD+F RQGRQLRRKMWLQSLQMKLMV G +   I+I+W + CGGFKC
Sbjct: 181 LVDKTENLQFQADSFQRQGRQLRRKMWLQSLQMKLMVAGAVFSFILIVWVVACGGFKC 238

BLAST of Lag0029675 vs. TAIR 10
Match: AT3G54300.2 (vesicle-associated membrane protein 727 )

HSP 1 Score: 401.7 bits (1031), Expect = 4.0e-112
Identity = 197/238 (82.77%), Postives = 219/238 (92.02%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHT +SGNFSTIAVQCLQ+LP+N +K TYSCDGHTFNFL+D
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTPYSGNFSTIAVQCLQKLPTNSSKYTYSCDGHTFNFLVD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GFVFL VADES GR++PFVFLERVK+DFK+RY +SIK++  HPLAD+++DDDLF DRFS
Sbjct: 61  NGFVFLVVADESTGRSVPFVFLERVKEDFKKRYEASIKNDERHPLADEDEDDDLFGDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAY LDREFGP LKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGE+IEL
Sbjct: 121 VAYNLDREFGPILKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENLQFQAD+F RQGRQLRRKMWLQSLQMKLMV G +   I+I+W + CGGFKC
Sbjct: 181 LVDKTENLQFQADSFQRQGRQLRRKMWLQSLQMKLMVAGAVFSFILIVWVVACGGFKC 238

BLAST of Lag0029675 vs. TAIR 10
Match: AT2G32670.1 (vesicle-associated membrane protein 725 )

HSP 1 Score: 276.9 bits (707), Expect = 1.5e-74
Identity = 136/238 (57.14%), Postives = 179/238 (75.21%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           M Q+ LIYSFVA+GTV+L E+T F GNF+ +A QCLQ+LPS+  K TY+CDGHTFN+L++
Sbjct: 66  MGQQNLIYSFVARGTVILVEYTEFKGNFTAVAAQCLQKLPSSNNKFTYNCDGHTFNYLVE 125

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  VA ESVGR +P  FLERVK+DF +RYG                         +
Sbjct: 126 NGFTYCVVAVESVGRQIPMAFLERVKEDFNKRYGGGKATT-------------------A 185

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L+REFG +LKEHMQYC+ HP+E+SKL+K+KAQ+TEVKG+MM+NIEKVLDRGE+IEL
Sbjct: 186 QANSLNREFGSKLKEHMQYCVDHPDEISKLAKVKAQVTEVKGVMMENIEKVLDRGEKIEL 245

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENL+ QA +F  QG ++RRKMW +++++KL+V G I+ LI+I+   VCGGFKC
Sbjct: 246 LVDKTENLRSQAQDFRTQGTKIRRKMWFENMKIKLIVLGIIITLILIIILSVCGGFKC 284

BLAST of Lag0029675 vs. TAIR 10
Match: AT1G04760.1 (vesicle-associated membrane protein 726 )

HSP 1 Score: 271.9 bits (694), Expect = 4.7e-73
Identity = 134/238 (56.30%), Postives = 176/238 (73.95%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           M Q+ LIYSFVA+GTV+LAE+T F GNF+++A QCLQ+LPS+  K TY+CDGHTFN+L D
Sbjct: 1   MGQQSLIYSFVARGTVILAEYTEFKGNFTSVAAQCLQKLPSSNNKFTYNCDGHTFNYLAD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  V  ES GR +P  FLERVK+DF +RYG                         +
Sbjct: 61  NGFTYCVVVIESAGRQIPMAFLERVKEDFNKRYGGGKAST-------------------A 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L++EFG +LKEHMQYC  HPEE+SKLSK+KAQ+TEVKG+MM+NIEKVLDRGE+IEL
Sbjct: 121 KANSLNKEFGSKLKEHMQYCADHPEEISKLSKVKAQVTEVKGVMMENIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENL+ QA +F  QG +++RK+W +++++KL+V G I+ LI+I+   VC GFKC
Sbjct: 181 LVDKTENLRSQAQDFRTQGTKMKRKLWFENMKIKLIVFGIIVALILIIILSVCHGFKC 219

BLAST of Lag0029675 vs. TAIR 10
Match: AT2G33120.1 (synaptobrevin-related protein 1 )

HSP 1 Score: 271.2 bits (692), Expect = 8.1e-73
Identity = 133/238 (55.88%), Postives = 177/238 (74.37%), Query Frame = 0

Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNGTKCTYSCDGHTFNFLLD 60
           M+Q+ LIYSFVA+GTV+L E T F GNF++IA QCLQ+LPS+  K TY+CDGHTFN+L++
Sbjct: 1   MAQQSLIYSFVARGTVILVEFTDFKGNFTSIAAQCLQKLPSSNNKFTYNCDGHTFNYLVE 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  VA +S GR +P  FLERVK+DF +RYG                         +
Sbjct: 61  NGFTYCVVAVDSAGRQIPMAFLERVKEDFNKRYGGG-------------------KAATA 120

Query: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L++EFG +LKEHMQYCM HP+E+SKL+K+KAQ++EVKG+MM+NIEKVLDRGE+IEL
Sbjct: 121 QANSLNKEFGSKLKEHMQYCMDHPDEISKLAKVKAQVSEVKGVMMENIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 239
           LVDKTENL+ QA +F  QG Q+RRKMW Q++++KL+V   I+ LI+I+   +CGGF C
Sbjct: 181 LVDKTENLRSQAQDFRTQGTQMRRKMWFQNMKIKLIVLAIIIALILIIILSICGGFNC 219

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038884037.19.8e-12998.32vesicle-associated membrane protein 727 [Benincasa hispida][more]
XP_008456603.19.8e-12998.74PREDICTED: vesicle-associated membrane protein 727 [Cucumis melo] >TYK11767.1 ve... [more]
XP_022939856.11.8e-12797.06vesicle-associated membrane protein 727-like [Cucurbita moschata] >XP_022939857.... [more]
XP_004140905.11.8e-12797.06vesicle-associated membrane protein 727 [Cucumis sativus] >KGN46041.1 hypothetic... [more]
XP_023551597.14.1e-12796.64vesicle-associated membrane protein 727-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9M3765.6e-11182.77Vesicle-associated membrane protein 727 OS=Arabidopsis thaliana OX=3702 GN=VAMP7... [more]
O488502.1e-7357.14Vesicle-associated membrane protein 725 OS=Arabidopsis thaliana OX=3702 GN=VAMP7... [more]
Q9MAS56.7e-7256.30Putative vesicle-associated membrane protein 726 OS=Arabidopsis thaliana OX=3702... [more]
P471921.1e-7155.88Vesicle-associated membrane protein 722 OS=Arabidopsis thaliana OX=3702 GN=VAMP7... [more]
Q9ZTW37.4e-7156.30Vesicle-associated membrane protein 721 OS=Arabidopsis thaliana OX=3702 GN=VAMP7... [more]
Match NameE-valueIdentityDescription
A0A5D3CIL24.7e-12998.74Vesicle-associated membrane protein 727 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3C3Q24.7e-12998.74vesicle-associated membrane protein 727 OS=Cucumis melo OX=3656 GN=LOC103496513 ... [more]
A0A6J1FGZ78.9e-12897.06vesicle-associated membrane protein 727-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A0A0KE698.9e-12897.06Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G045200 PE=3 SV=1[more]
A0A6J1JTB22.0e-12796.64vesicle-associated membrane protein 727-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT3G54300.14.0e-11282.77vesicle-associated membrane protein 727 [more]
AT3G54300.24.0e-11282.77vesicle-associated membrane protein 727 [more]
AT2G32670.11.5e-7457.14vesicle-associated membrane protein 725 [more]
AT1G04760.14.7e-7356.30vesicle-associated membrane protein 726 [more]
AT2G33120.18.1e-7355.88synaptobrevin-related protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001388SynaptobrevinPRINTSPR00219SYNAPTOBREVNcoord: 154..173
score: 50.41
coord: 210..229
score: 24.32
coord: 174..193
score: 42.43
IPR001388SynaptobrevinPFAMPF00957Synaptobrevincoord: 148..234
e-value: 9.5E-32
score: 108.6
IPR001388SynaptobrevinPROSITEPS00417SYNAPTOBREVINcoord: 167..186
IPR010908Longin domainSMARTSM01270Longin_2coord: 31..111
e-value: 1.9E-22
score: 90.6
IPR010908Longin domainPFAMPF13774Longincoord: 33..100
e-value: 1.0E-17
score: 63.9
IPR010908Longin domainPROSITEPS50859LONGINcoord: 6..133
score: 23.776814
IPR010908Longin domainCDDcd14824Longincoord: 7..140
e-value: 5.26827E-33
score: 114.275
NoneNo IPR availableGENE3D1.20.5.110coord: 123..233
e-value: 2.3E-34
score: 119.6
NoneNo IPR availableGENE3D3.30.450.50Longin domaincoord: 1..111
e-value: 3.2E-38
score: 133.2
NoneNo IPR availablePANTHERPTHR21136:SF169VESICLE-ASSOCIATED MEMBRANE PROTEIN 727coord: 1..238
NoneNo IPR availablePANTHERPTHR21136SNARE PROTEINScoord: 1..238
NoneNo IPR availableCDDcd15843R-SNAREcoord: 149..207
e-value: 1.12098E-29
score: 103.736
NoneNo IPR availableSUPERFAMILY58038SNARE fusion complexcoord: 144..209
IPR042855v-SNARE, coiled-coil homology domainPROSITEPS50892V_SNAREcoord: 149..209
score: 19.343924
IPR011012Longin-like domain superfamilySUPERFAMILY64356SNARE-likecoord: 6..98

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0029675.1Lag0029675.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle