Lag0029402 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0029402
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr8: 38615386 .. 38616854 (+)
RNA-Seq ExpressionLag0029402
SyntenyLag0029402
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGCCTGATTTGGGGGAGGGAGCTATTGGGAAAGGGTATTCGTTGGAGGGTTGGGAATGGGGAGAAAGTCAGTGTATATGGATCTAACTGGATTCCAGATGATTTCTGTCTCAGGGTACGGTCCCCGGTTACTTTGGATCCTGGTGCTCGAGTGGCTGATCTTCTTACAGCCTCGGGCCAGTGGAATGAGGAACTCCTGCGACATCATTTTAGCCCTCGTGAGGTACGCTCTATCCTTACTATCCCACTGCGGCATATTTTGTCTGAGGATATAGTCATTTGGCATTTTGAGAAGTGTGGGAGGTTAAAAGTGGGTACCGGCTTGGCCAAAGGGCTTTGTCTGCCCATTCTCCATCTTCGTCTTCGAGTGAGGCGTTGTCTAGCTGGTGGAAGGGGTGTTGGAAGATGGGGATCCCAAACAAAATCAAGGTTTTCTTTTGGAGGCTTTGTTTGGAGCGACTGCCTACTGTTGACAATTTGAGTACTAGGGGCGTTGATGTCTTGAATGTGTGTGTGCACTGTAGTTGGCATGGGGAGTCATGCATGCATGTGTTTTGGCAATGCAAATATTTTTGTGAATTGTTGATGGGATCGGAGTGGGAGGTCTTGCTGCAGAATATCCAGGCAAACTCTATGCTTAATCTGCTTAGGGATGTGAAGGATAGGGTCGACTGGGCTAAGTTTGAAGAGCTTGTGGTGGTGTTGTGGGTTGTATGGTGTTGCCGAAACCAACAGAAGTTTAGGGGGCGGGCCCCTCAGCAGGGCTTGTGGATTGGGCGACGAACTATCTGGCTGTTTTCCGAGGGGCCTCCCGGACGTATGGCGAGGGTGTTGGAGGGGTGCCTCGAAGTGCAGTAAAATGGATCCCGCCGGAGAAGGGATGGTATAAGGCGAACGTGGATGCTGCATACTGTGGGGACCTGTCTCGAGCGGGTGTTGGAGTTGTTGTTCGGGATGAGGTGGGGCGAGTCATGCTGTCAGCTGCTGTTAGTCATGACCATGTGGGGAGTTCAGATTTGGCAGAGGGTCTAGCGGTGGTGGACGGAATGAGACTTGTGGTGGAGATGGGTTTTGCTCGAGTTATCCTTGAGACTGACTCTAGCGGGTTTTTTCGCTGTTGCAAGATCCTGCGATGGTGGATCTGTCTGAGGTCGGTGTTCTAGTTTCTGAGGCTCGAAGGGAGGTGTCTGCACATATGCAGTTCAGGTACAGCTTTACAAGAAGAGAAGGAAACCGGGTTGCCCATGAGCTAGCTCGCTTTGCATTGAGAGAGGGAGGCTGTGAGTATTGGATGGAATTGGTTCCGGTGTGTGCTTATCTTCCAGCTGAGGATGATATTTTTTCCCTATAACTTTTCTCTTTGTGTGCTTATGTCAGGCAGGTGGCGGCGGAAGCGTGTCATGGATTATGTGATTTACTCTAAAGCTAGTGGAGATCGAGTTTTGAAAGCAAACCAGAGAACTATCTAA

mRNA sequence

AGCCTGATTTGGGGGAGGGAGCTATTGGGAAAGGGTATTCGTTGGAGGGTTGGGAATGGGGAGAAAGTCAGTGTATATGGATCTAACTGGATTCCAGATGATTTCTGTCTCAGGGTACGGTCCCCGGTTACTTTGGATCCTGGTGCTCGAGTGGCTGATCTTCTTACAGCCTCGGGCCAGTGGAATGAGGAACTCCTGCGACATCATTTTAGCCCTCGTGAGGTACGCTCTATCCTTACTATCCCACTGCGGCATATTTTGTCTGAGGATATAGTCATTTGGCATTTTGAGAAGTGTGGGAGGTTAAAAGTGGGTACCGGCTTGGCCAAAGGGCTTTGTCTGCCCATTCTCCATCTTCGTCTTCGAGTGAGGCGTTGTCTAGCTGGTGGAAGGGGTGTTGGAAGATGGGGATCCCAAACAAAATCAAGTTGGCATGGGGAGTCATGCATGCATGTGTTTTGGCAATGCAAATATTTTTGTGAATTGTTGATGGGATCGGAGTGGGAGGTCTTGCTGCAGAATATCCAGGCAAACTCTATGCTTAATCTGCTTAGGGATGTGAAGGATAGGGTCGACTGGGCTAAGTTTGAAGAGCTTGTGGTGGGGGCGGGCCCCTCAGCAGGGCTTGTGGATTGGGCGACGAACTATCTGGCTGTTTTCCGAGGGGCCTCCCGGACGTATGGCGAGGGTGTTGGAGGGGTGCCTCGAAGTGCAGTAAAATGGATCCCGCCGGAGAAGGGATGGTATAAGGCGAACGTGGATGCTGCATACTGTGGGGACCTGTCTCGAGCGGGTGTTGGAGTTGTTGTTCGGGATGAGGTGGGGCGAGTCATGCTGTCAGCTGCTGTTAGTCATGACCATGTGGGGAGTTCAGATTTGGCAGAGGGTCTAGCGGTGGTGGACGGAATGAGACTTGTGGTGGAGATGGGTTTTGCTCGAGTTATCCTTGAGACTGACTCTAGCGGGTTTTTTCGCTGTTGCAAGATCCTGCGATGGTGGATCTGTCTGAGGTACAGCTTTACAAGAAGAGAAGGAAACCGGGTTGCCCATGAGCTAGCTCGCTTTGCATTGAGAGAGGGAGGCTGCAGGTGGCGGCGGAAGCGTGTCATGGATTATGTGATTTACTCTAAAGCTAGTGGAGATCGAGTTTTGAAAGCAAACCAGAGAACTATCTAA

Coding sequence (CDS)

GAGCCTGATTTGGGGGAGGGAGCTATTGGGAAAGGGTATTCGTTGGAGGGTTGGGAATGGGGAGAAAGTCAGTGTATATGGATCTAACTGGATTCCAGATGATTTCTGTCTCAGGGTACGGTCCCCGGTTACTTTGGATCCTGGTGCTCGAGTGGCTGATCTTCTTACAGCCTCGGGCCAGTGGAATGAGGAACTCCTGCGACATCATTTTAGCCCTCGTGAGGTACGCTCTATCCTTACTATCCCACTGCGGCATATTTTGTCTGAGGATATAGTCATTTGGCATTTTGAGAAGTGTGGGAGGTTAAAAGTGGGTACCGGCTTGGCCAAAGGGCTTTGTCTGCCCATTCTCCATCTTCGTCTTCGAGTGAGGCGTTGTCTAGCTGGTGGAAGGGGTGTTGGAAGATGGGGATCCCAAACAAAATCAAGTTGGCATGGGGAGTCATGCATGCATGTGTTTTGGCAATGCAAATATTTTTGTGAATTGTTGATGGGATCGGAGTGGGAGGTCTTGCTGCAGAATATCCAGGCAAACTCTATGCTTAATCTGCTTAGGGATGTGAAGGATAGGGTCGACTGGGCTAAGTTTGAAGAGCTTGTGGTGGGGGCGGGCCCCTCAGCAGGGCTTGTGGATTGGGCGACGAACTATCTGGCTGTTTTCCGAGGGGCCTCCCGGACGTATGGCGAGGGTGTTGGAGGGGTGCCTCGAAGTGCAGTAAAATGGATCCCGCCGGAGAAGGGATGGTATAAGGCGAACGTGGATGCTGCATACTGTGGGGACCTGTCTCGAGCGGGTGTTGGAGTTGTTGTTCGGGATGAGGTGGGGCGAGTCATGCTGTCAGCTGCTGTTAGTCATGACCATGTGGGGAGTTCAGATTTGGCAGAGGGTCTAGCGGTGGTGGACGGAATGAGACTTGTGGTGGAGATGGGTTTTGCTCGAGTTATCCTTGAGACTGACTCTAGCGGGTTTTTTCGCTGTTGCAAGATCCTGCGATGGTGGATCTGTCTGAGGTACAGCTTTACAAGAAGAGAAGGAAACCGGGTTGCCCATGAGCTAGCTCGCTTTGCATTGAGAGAGGGAGGCTGCAGGTGGCGGCGGAAGCGTGTCATGGATTATGTGATTTACTCTAAAGCTAGTGGAGATCGAGTTTTGAAAGCAAACCAGAGAACTATCTAA

Protein sequence

SLIWGRELLGKGIRWRVGNGEKVSVYGSNWIPDDFCLRVRSPVTLDPGARVADLLTASGQWNEELLRHHFSPREVRSILTIPLRHILSEDIVIWHFEKCGRLKVGTGLAKGLCLPILHLRLRVRRCLAGGRGVGRWGSQTKSSWHGESCMHVFWQCKYFCELLMGSEWEVLLQNIQANSMLNLLRDVKDRVDWAKFEELVVGAGPSAGLVDWATNYLAVFRGASRTYGEGVGGVPRSAVKWIPPEKGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVMLSAAVSHDHVGSSDLAEGLAVVDGMRLVVEMGFARVILETDSSGFFRCCKILRWWICLRYSFTRREGNRVAHELARFALREGGCRWRRKRVMDYVIYSKASGDRVLKANQRTI
Homology
BLAST of Lag0029402 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 183.7 bits (465), Expect = 3.0e-42
Identity = 123/417 (29.50%), Postives = 198/417 (47.48%), Query Frame = 0

Query: 1    SLIWGRELLGKGIRWRVGNGEKVSVYGSNWIPDDFCLRVRSPVTLDPGARVADLLT-ASG 60
            S++WGR+LL KG+RWR+GNG+ V +YG NW+P+   L++ S   L   +RV+ L+    G
Sbjct: 703  SILWGRDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLVSRVSSLVDHEEG 762

Query: 61   QWNEELLRHHFSPREVRSILTIPLRHILSEDIVIWHFEKCGRLKVGTG----LAKGLCLP 120
             W  +++R  F+P E + IL+IP+     ED +IW++EK G   V +G    L    C+ 
Sbjct: 763  GWQGDVVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYKVALLNNPCVQ 822

Query: 121  I------------------LHLRLRVR-------------RCLAGGRGVGRWGSQTKSSW 180
                               +H+  +++              C    RGV           
Sbjct: 823  APSSSSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGR 882

Query: 181  HGESCMHVFWQCKYFCELLMGSEWEVLLQNIQANSMLNLLRDVKDRVDWAKFEELVVGAG 240
            +GE  +H+FW CK+   L + S++  L       S   +LR+  + +  A FEEL V   
Sbjct: 883  NGEDSIHLFWICKFAEALWINSKFGKL-------SPFLILRESHESLSKADFEELCVVIW 942

Query: 241  P--------------------SAGLVDWATNYLAVFRGASRTYGEGVGGVPRSA-VKWIP 300
                                    LV+WA  Y   FR A        G V  +A + W P
Sbjct: 943  GLWNQRNARAFNDSTKTVFKIGMELVEWANKYAMEFREAKS--NPITGRVTNTAEILWQP 1002

Query: 301  PEKGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVMLSAAVSHDHVGSSDLAEGLAVVDGM 358
            P++G YK N DA++      AG+G+++ ++ G+VM +A    +++ S D+AE +A V+G+
Sbjct: 1003 PDEGIYKINTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMAEAIAAVEGL 1062

BLAST of Lag0029402 vs. NCBI nr
Match: XP_042944602.1 (uncharacterized protein LOC122278481 [Carya illinoinensis])

HSP 1 Score: 146.0 bits (367), Expect = 6.9e-31
Identity = 121/406 (29.80%), Postives = 188/406 (46.31%), Query Frame = 0

Query: 1   SLIWGRELLGKGIRWRVGNGEKVSVYGSNWIPDDFCLRVRSPV-TLDPGARVADLLTASG 60
           SL+  ++L+  G+ WRVGNG+ +S++ + W+P+    +VRSP+ TL   ++V+ L+    
Sbjct: 398 SLLHAQDLVKAGMVWRVGNGKSISIWNNKWLPNGSDFKVRSPINTLPADSKVSSLIFEDL 457

Query: 61  Q-WNEELLRHHFSPREVRSILTIPLRHILSEDIVIWHFEKCGRLKV------GTGLAKGL 120
             WNE L+   FS  E   I +IPL     ED ++W F K GR  V      G  + +  
Sbjct: 458 HCWNERLVGDMFSKEEAAQICSIPLSWHGEEDRILWGFTKDGRFSVRSAYHLGNDMGRSR 517

Query: 121 CLPILHLRLRVRRCLAGGRGVGRWGSQTKSSWHG--ESCMHVFWQC-------------- 180
               L   L  R+ L   + +      T +  HG  E+  HV W C              
Sbjct: 518 LGNALKGILPTRQNLVQRKII---EDATCNICHGDVETVCHVLWSCPAASDVWAESGSGL 577

Query: 181 -KYFCE----LLMGSEWEVLLQNIQANSMLNLLRDVKDRVDWAKFEELVVGAGPSAGLVD 240
            K+ CE      + S+ +  LQ  +   +  +LR +  R +   FE       PS  L +
Sbjct: 578 QKWICEEKDFFQVWSDMQTRLQQSKLEEVAMILRGLWTRRNMMIFEGKF--KKPSKVLNE 637

Query: 241 WATNYLAVFRGASRTYGEGVGGV--PRSAVKWIPPEKGWYKANVDAAYCGDLSRAGVGVV 300
             T+ L  ++ A     +  G +   R   KW+PP++G  K N DAA      R G+G+ 
Sbjct: 638 ALTS-LRNYKSAHAELNQMKGPMHQTRKDTKWMPPDEGISKINFDAAIDKTNYRLGLGIA 697

Query: 301 VRDEVGRVMLSAAVSHDHVGSSDLAEGLAVVDGMRLVVEMGFARVILETDSSGFFRCCKI 360
             + VG V+ S + S    GSSD+ E  A+   M LV+E+    V+ E D++      ++
Sbjct: 698 GTNHVGEVLFSFSCSKLFSGSSDMGEAAALWRAMELVMELDVRNVVFEGDAN------RV 757

Query: 361 LRWWICLRYSFTR----------REGNRVAHELARFALR-EGGCRW 365
           ++    +R ++ R          REGN VAHELA+ ALR EG   W
Sbjct: 758 IKGVTDVRGNYARMEQMYANIRSREGNCVAHELAKRALRMEGEWCW 791

BLAST of Lag0029402 vs. NCBI nr
Match: TXG71894.1 (hypothetical protein EZV62_000473 [Acer yangbiense])

HSP 1 Score: 143.3 bits (360), Expect = 4.5e-30
Identity = 115/393 (29.26%), Postives = 171/393 (43.51%), Query Frame = 0

Query: 8   LLGKGIRWRVGNGEKVSVYGSNWIPDDFCLRVRSPVTLDPGARVADLLTASGQWNEELLR 67
           +L +G R R+G+G    +Y   W+P     +V SP  L   + V+ L+ + G+WNE L+R
Sbjct: 1   MLNRGSRRRIGDGNDTLIYEDQWLPMPSTFKVCSPKRLPDASVVSTLIESPGKWNESLIR 60

Query: 68  HHFSPREVRSILTIPLRHILSEDIVIWHFEKCGRLKVGTGLAKGLCLPILHLRLRVRRCL 127
            +F P E   IL+IPL   L  D  +WHF K G   V +       + I  ++     C 
Sbjct: 61  RNFLPEEAELILSIPLSVYLRRDSFLWHFNKDGTFTVKSAYR----VAISAMKRFEAFCS 120

Query: 128 AGGRGVGRWGSQTKSSWHGESCMHVFWQCKYFCELLMGSEWEVLLQNIQANSMLNLLR-- 187
           +    + R G +T          H  W+CK      +  EW     +    S LN L+  
Sbjct: 121 SALCPLCREGPETVE--------HALWRCK-----SVKPEW----NHCPLFSDLNKLKID 180

Query: 188 DVKDRVDWAKFE---ELVVGAGPSAGLVDWATNYLAVFRGASRTYGEG--------VGG- 247
           D   RV W       E VV    +A LV W     ++FR  ++ +G+          GG 
Sbjct: 181 DFLGRVIWVASVANLENVVNFITTAWLV-WNNRNQSLFREKAKCWGDSWTKATLFLSGGK 240

Query: 248 ----------VPRSAVK-WIPPEKGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVMLSAA 307
                     +P   +K WIPP  G  K NVDAA     ++  VG+V+RD  GR++ SAA
Sbjct: 241 TIETQNNSSAIPSLVIKNWIPPSFGTIKVNVDAALDVGRNKFSVGIVIRDSSGRILKSAA 300

Query: 308 VSHDHVGSSDLAEGLAVVDGMRLVVEMGFARVILETDSSGFFRCCK-------------- 358
           +  +   S+D+AE   + +G+ L    G   + +E+DS    R C               
Sbjct: 301 LVFNGRTSTDIAEAKVIYEGLLLATSSGLQPLSIESDSLNVVRLCNGEISTRNDVFNVIC 360

BLAST of Lag0029402 vs. NCBI nr
Match: CAB4262994.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 143.3 bits (360), Expect = 4.5e-30
Identity = 106/343 (30.90%), Postives = 155/343 (45.19%), Query Frame = 0

Query: 1    SLIWGRELLGKGIRWRVGNGEKVSVYGSNWIPDDFCLRVRSPVTLDPGARVADLLTASGQ 60
            SL WG+ELL KG+RWRVGNGE + VY + W+P     ++ S   L     V DL T+SGQ
Sbjct: 1303 SLQWGKELLNKGLRWRVGNGESIQVYTNKWLPVPSFFKIMSAPQLPLSTLVCDLFTSSGQ 1362

Query: 61   WNEELLRHHFSPREVRSILTIPLRHILSEDIVIWHFEKCGRLKVGTGLAKGLCLPILHLR 120
            WN  LL+  F  +EV +IL IPL  + S D +IWH+E+ G   V +G        +  L 
Sbjct: 1363 WNVPLLKDIFWDQEVEAILQIPLVSLASHDCLIWHYERNGMYSVKSG------YQLTRLE 1422

Query: 121  LRVRRCLAGGRGVGRWGSQTKSSWHGESCMHVFWQCKYFCELLMGSEWEVLLQNIQANSM 180
              +  C     GV       K  W     + +  + K+F     G+    L  N+   S 
Sbjct: 1423 KDI-MCGESSAGVNLSSRFWKKIW----ALKIPNKIKFFLWRRCGA--TQLGGNVCEVSR 1482

Query: 181  LNLLRDVKDRVDWAK-FEELVVGAGPSAGLVDWATNYLAVFRGASRTY------------ 240
            +N  R++   V  +   EE  + A    GL +W  + +   +  + T             
Sbjct: 1483 VNSFRELWYAVQISSGGEEQGLFAYLRWGLWNWRDSLIFEAKSETATQLLHRMHTLAQEF 1542

Query: 241  ---------GEGVGGVPRSAVK-WIPPEKGWYKANVDAAYCGDLSRAGVGVVVRDEVGRV 300
                       G    P++++  W PP  G YK NVD A     S  GVGVVVR+E G  
Sbjct: 1543 FDVNNSSHTSHGRQSSPQASLHVWRPPAVGIYKINVDRALKSGDSIRGVGVVVRNENGDF 1602

Query: 301  MLSAAVSHDHVGSSDLAEGLAVVDGMRLVVEMGFARVILETDS 321
            M +          +   E +A ++G+R  ++MGF   +LE D+
Sbjct: 1603 MAACVRRIQASYGARQMELMATIEGLRFAIDMGFTDAVLEMDA 1632

BLAST of Lag0029402 vs. NCBI nr
Match: VVA32947.1 (PREDICTED: retrotransposon [Prunus dulcis])

HSP 1 Score: 140.6 bits (353), Expect = 2.9e-29
Identity = 109/380 (28.68%), Postives = 156/380 (41.05%), Query Frame = 0

Query: 1    SLIWGRELLGKGIRWRVGNGEKVSVYGSNWIPDDFCLRVRSPVTLDPGARVADLLTASGQ 60
            SL WG+ELL KG+RWRVGNG  + VY   W+P     ++ SP  L     V DL T+SGQ
Sbjct: 842  SLQWGKELLNKGLRWRVGNGVSIQVYTDKWLPAPSFFKIMSPPQLPLSTLVCDLFTSSGQ 901

Query: 61   WNEELLRHHFSPREVRSILTIPLRHILSEDIVIWHFEKCGRLKVGTGLAKGLCL------ 120
            WN  LL+  F  +EV + L IPL  +   D +IWH+E+ G   V +G  +  CL      
Sbjct: 902  WNVPLLKDIFWDQEVDAKLQIPLASLAGHDCLIWHYERNGMYSVKSGY-RLACLEKDKMS 961

Query: 121  --PILHLRLRVR-------------------RC---------LAGGRGVGRWGSQTKSSW 180
              P + + L  +                   RC         +   R +           
Sbjct: 962  GEPSVRVDLNSKFWKKIWALKIPNKIKFFLWRCAWDFLPCGQILFNRKIAPTPICPNCHR 1021

Query: 181  HGESCMHVFWQCKYFCELLMGSEWEVLLQNIQANSMLNLLRDVKDRVDWAKFEELVVGAG 240
              ES +H  W C+   E+   S W  + +  + NS   L   ++     +  EE  + A 
Sbjct: 1022 KAESVLHAVWLCETAKEVWRNSAWGNVCEEWRVNSFRELWHALQLS---SSGEEQGLFAY 1081

Query: 241  PSAGLVDWATNYLAVFRGASRTYGE-----------------------GVGGVPRSAVK- 300
               GL  W      +F G S T  +                       G    P++ +  
Sbjct: 1082 LCWGL--WNRRNSFIFEGKSETATQLLHRMTKLAQEFSNANNLSHTIHGRQSSPQAPLHG 1141

Query: 301  WIPPEKGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVMLSAAVSHDHVGSSDLAEGLAVV 321
            W PP  G YK NVD A     S  GVGVVVR+  G  M +          +   E +A +
Sbjct: 1142 WRPPPAGIYKINVDGAVKSGDSVRGVGVVVRNANGEFMAACVRRIQASYGARQTELMATI 1201

BLAST of Lag0029402 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 1.5e-42
Identity = 123/417 (29.50%), Postives = 198/417 (47.48%), Query Frame = 0

Query: 1    SLIWGRELLGKGIRWRVGNGEKVSVYGSNWIPDDFCLRVRSPVTLDPGARVADLLT-ASG 60
            S++WGR+LL KG+RWR+GNG+ V +YG NW+P+   L++ S   L   +RV+ L+    G
Sbjct: 703  SILWGRDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLVSRVSSLVDHEEG 762

Query: 61   QWNEELLRHHFSPREVRSILTIPLRHILSEDIVIWHFEKCGRLKVGTG----LAKGLCLP 120
             W  +++R  F+P E + IL+IP+     ED +IW++EK G   V +G    L    C+ 
Sbjct: 763  GWQGDVVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYKVALLNNPCVQ 822

Query: 121  I------------------LHLRLRVR-------------RCLAGGRGVGRWGSQTKSSW 180
                               +H+  +++              C    RGV           
Sbjct: 823  APSSSSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGR 882

Query: 181  HGESCMHVFWQCKYFCELLMGSEWEVLLQNIQANSMLNLLRDVKDRVDWAKFEELVVGAG 240
            +GE  +H+FW CK+   L + S++  L       S   +LR+  + +  A FEEL V   
Sbjct: 883  NGEDSIHLFWICKFAEALWINSKFGKL-------SPFLILRESHESLSKADFEELCVVIW 942

Query: 241  P--------------------SAGLVDWATNYLAVFRGASRTYGEGVGGVPRSA-VKWIP 300
                                    LV+WA  Y   FR A        G V  +A + W P
Sbjct: 943  GLWNQRNARAFNDSTKTVFKIGMELVEWANKYAMEFREAKS--NPITGRVTNTAEILWQP 1002

Query: 301  PEKGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVMLSAAVSHDHVGSSDLAEGLAVVDGM 358
            P++G YK N DA++      AG+G+++ ++ G+VM +A    +++ S D+AE +A V+G+
Sbjct: 1003 PDEGIYKINTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMAEAIAAVEGL 1062

BLAST of Lag0029402 vs. ExPASy TrEMBL
Match: M5XJH9 (Uncharacterized protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa023013mg PE=4 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 5.2e-32
Identity = 116/379 (30.61%), Postives = 181/379 (47.76%), Query Frame = 0

Query: 1   SLIWGRELLGKGIRWRVGNGEKVSVYGSNWIPDDFCLRVRSPVTLDPGARVADLLTASGQ 60
           SLIWG ELL  G+R R+G+G++  VYG  WIP     R  SP  LD   +V+D +   G 
Sbjct: 210 SLIWGHELLLSGLRKRIGDGQETLVYGDAWIPRPNSFRPISPQVLDQETKVSDFIFPIGN 269

Query: 61  WNEELLRHHFSPREVRSILTIPLRHILSEDIVIWHFEKCGRLKVGTGL-----AKGLCLP 120
           WN +LL   F   +V++I +IPL     +D  +WH+   G   V +G       K  C  
Sbjct: 270 WNVDLLNLCFHEEDVKAITSIPLSVNYHKDRWMWHYTTNGIYSVKSGYRLEMSKKKECSG 329

Query: 121 ILHLRLRVRRCLAGGRGVGRWGSQTKSSWHGESCMHVFWQC-KYFCELLMGSEWEVLLQ- 180
           ++    + R   A  R V  W  + +     +  ++  W+  K +   +       ++  
Sbjct: 330 VVGSSSKPRVRNAFWRKV--WSQEVR-----QKILYFNWRAIKNYLPCISNMLKRKIIYD 389

Query: 181 ------NIQANSMLNLLRDVKD-RVDWAK--FEELVVGAGPS--AGLVDWATNYLAVFRG 240
                 N+ + S+++ L    + +  W K  F E+  G   S    L + AT YL+  R 
Sbjct: 390 DSCPICNVNSESVIHALWSCPNAQKVWKKVLFREVFTGLNFSNHGDLFEIATMYLSKARY 449

Query: 241 ASRTYGEGVGGVPRSAV----KWIPPEKGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVM 300
               YG  V G P+  V    KW PP  G YK NVDA +  +     +GVVVR++ G V 
Sbjct: 450 LVGEYGSLVKGEPKLIVEIPTKWSPPPVGKYKLNVDAVFIPETGVGEIGVVVRNDKGGV- 509

Query: 301 LSAAVSHDHVGSSDLAEGLAVVDGMRLVVEMGFARVILETDSSGFFRCCKILRWWICLRY 358
           ++ A+      SS  AE +  + G++   +  F+ + +E+DS G     + L+++  +  
Sbjct: 510 VAMALPLASAASSKHAEIMTFLFGIKFTRDASFSSIFIESDSQGVVN-DRSLQYFEDVII 569

BLAST of Lag0029402 vs. ExPASy TrEMBL
Match: A0A5C7IST2 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_000473 PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.2e-30
Identity = 115/393 (29.26%), Postives = 171/393 (43.51%), Query Frame = 0

Query: 8   LLGKGIRWRVGNGEKVSVYGSNWIPDDFCLRVRSPVTLDPGARVADLLTASGQWNEELLR 67
           +L +G R R+G+G    +Y   W+P     +V SP  L   + V+ L+ + G+WNE L+R
Sbjct: 1   MLNRGSRRRIGDGNDTLIYEDQWLPMPSTFKVCSPKRLPDASVVSTLIESPGKWNESLIR 60

Query: 68  HHFSPREVRSILTIPLRHILSEDIVIWHFEKCGRLKVGTGLAKGLCLPILHLRLRVRRCL 127
            +F P E   IL+IPL   L  D  +WHF K G   V +       + I  ++     C 
Sbjct: 61  RNFLPEEAELILSIPLSVYLRRDSFLWHFNKDGTFTVKSAYR----VAISAMKRFEAFCS 120

Query: 128 AGGRGVGRWGSQTKSSWHGESCMHVFWQCKYFCELLMGSEWEVLLQNIQANSMLNLLR-- 187
           +    + R G +T          H  W+CK      +  EW     +    S LN L+  
Sbjct: 121 SALCPLCREGPETVE--------HALWRCK-----SVKPEW----NHCPLFSDLNKLKID 180

Query: 188 DVKDRVDWAKFE---ELVVGAGPSAGLVDWATNYLAVFRGASRTYGEG--------VGG- 247
           D   RV W       E VV    +A LV W     ++FR  ++ +G+          GG 
Sbjct: 181 DFLGRVIWVASVANLENVVNFITTAWLV-WNNRNQSLFREKAKCWGDSWTKATLFLSGGK 240

Query: 248 ----------VPRSAVK-WIPPEKGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVMLSAA 307
                     +P   +K WIPP  G  K NVDAA     ++  VG+V+RD  GR++ SAA
Sbjct: 241 TIETQNNSSAIPSLVIKNWIPPSFGTIKVNVDAALDVGRNKFSVGIVIRDSSGRILKSAA 300

Query: 308 VSHDHVGSSDLAEGLAVVDGMRLVVEMGFARVILETDSSGFFRCCK-------------- 358
           +  +   S+D+AE   + +G+ L    G   + +E+DS    R C               
Sbjct: 301 LVFNGRTSTDIAEAKVIYEGLLLATSSGLQPLSIESDSLNVVRLCNGEISTRNDVFNVIC 360

BLAST of Lag0029402 vs. ExPASy TrEMBL
Match: A0A6J5TGK7 (CCHC-type domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS2532 PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.2e-30
Identity = 106/343 (30.90%), Postives = 155/343 (45.19%), Query Frame = 0

Query: 1    SLIWGRELLGKGIRWRVGNGEKVSVYGSNWIPDDFCLRVRSPVTLDPGARVADLLTASGQ 60
            SL WG+ELL KG+RWRVGNGE + VY + W+P     ++ S   L     V DL T+SGQ
Sbjct: 1303 SLQWGKELLNKGLRWRVGNGESIQVYTNKWLPVPSFFKIMSAPQLPLSTLVCDLFTSSGQ 1362

Query: 61   WNEELLRHHFSPREVRSILTIPLRHILSEDIVIWHFEKCGRLKVGTGLAKGLCLPILHLR 120
            WN  LL+  F  +EV +IL IPL  + S D +IWH+E+ G   V +G        +  L 
Sbjct: 1363 WNVPLLKDIFWDQEVEAILQIPLVSLASHDCLIWHYERNGMYSVKSG------YQLTRLE 1422

Query: 121  LRVRRCLAGGRGVGRWGSQTKSSWHGESCMHVFWQCKYFCELLMGSEWEVLLQNIQANSM 180
              +  C     GV       K  W     + +  + K+F     G+    L  N+   S 
Sbjct: 1423 KDI-MCGESSAGVNLSSRFWKKIW----ALKIPNKIKFFLWRRCGA--TQLGGNVCEVSR 1482

Query: 181  LNLLRDVKDRVDWAK-FEELVVGAGPSAGLVDWATNYLAVFRGASRTY------------ 240
            +N  R++   V  +   EE  + A    GL +W  + +   +  + T             
Sbjct: 1483 VNSFRELWYAVQISSGGEEQGLFAYLRWGLWNWRDSLIFEAKSETATQLLHRMHTLAQEF 1542

Query: 241  ---------GEGVGGVPRSAVK-WIPPEKGWYKANVDAAYCGDLSRAGVGVVVRDEVGRV 300
                       G    P++++  W PP  G YK NVD A     S  GVGVVVR+E G  
Sbjct: 1543 FDVNNSSHTSHGRQSSPQASLHVWRPPAVGIYKINVDRALKSGDSIRGVGVVVRNENGDF 1602

Query: 301  MLSAAVSHDHVGSSDLAEGLAVVDGMRLVVEMGFARVILETDS 321
            M +          +   E +A ++G+R  ++MGF   +LE D+
Sbjct: 1603 MAACVRRIQASYGARQMELMATIEGLRFAIDMGFTDAVLEMDA 1632

BLAST of Lag0029402 vs. ExPASy TrEMBL
Match: A0A5E4FZN9 (PREDICTED: retrotransposon OS=Prunus dulcis OX=3755 GN=ALMOND_2B007697 PE=4 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 1.4e-29
Identity = 109/380 (28.68%), Postives = 156/380 (41.05%), Query Frame = 0

Query: 1    SLIWGRELLGKGIRWRVGNGEKVSVYGSNWIPDDFCLRVRSPVTLDPGARVADLLTASGQ 60
            SL WG+ELL KG+RWRVGNG  + VY   W+P     ++ SP  L     V DL T+SGQ
Sbjct: 842  SLQWGKELLNKGLRWRVGNGVSIQVYTDKWLPAPSFFKIMSPPQLPLSTLVCDLFTSSGQ 901

Query: 61   WNEELLRHHFSPREVRSILTIPLRHILSEDIVIWHFEKCGRLKVGTGLAKGLCL------ 120
            WN  LL+  F  +EV + L IPL  +   D +IWH+E+ G   V +G  +  CL      
Sbjct: 902  WNVPLLKDIFWDQEVDAKLQIPLASLAGHDCLIWHYERNGMYSVKSGY-RLACLEKDKMS 961

Query: 121  --PILHLRLRVR-------------------RC---------LAGGRGVGRWGSQTKSSW 180
              P + + L  +                   RC         +   R +           
Sbjct: 962  GEPSVRVDLNSKFWKKIWALKIPNKIKFFLWRCAWDFLPCGQILFNRKIAPTPICPNCHR 1021

Query: 181  HGESCMHVFWQCKYFCELLMGSEWEVLLQNIQANSMLNLLRDVKDRVDWAKFEELVVGAG 240
              ES +H  W C+   E+   S W  + +  + NS   L   ++     +  EE  + A 
Sbjct: 1022 KAESVLHAVWLCETAKEVWRNSAWGNVCEEWRVNSFRELWHALQLS---SSGEEQGLFAY 1081

Query: 241  PSAGLVDWATNYLAVFRGASRTYGE-----------------------GVGGVPRSAVK- 300
               GL  W      +F G S T  +                       G    P++ +  
Sbjct: 1082 LCWGL--WNRRNSFIFEGKSETATQLLHRMTKLAQEFSNANNLSHTIHGRQSSPQAPLHG 1141

Query: 301  WIPPEKGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVMLSAAVSHDHVGSSDLAEGLAVV 321
            W PP  G YK NVD A     S  GVGVVVR+  G  M +          +   E +A +
Sbjct: 1142 WRPPPAGIYKINVDGAVKSGDSVRGVGVVVRNANGEFMAACVRRIQASYGARQTELMATI 1201

BLAST of Lag0029402 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 47.8 bits (112), Expect = 2.4e-05
Identity = 34/134 (25.37%), Postives = 57/134 (42.54%), Query Frame = 0

Query: 238 AVKWIPPEKGWYKANVDAAYCGDLSRAGVGVVVRDEVGRVMLSAAVSHDHVGSSDLAEGL 297
           +V+W  P   W K N DA +  +  R G+G ++R+E G V+   A +     +   AE  
Sbjct: 134 SVQWKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAELE 193

Query: 298 AVVDGMRLVVEMGFARVILETDSSGFFRCCKILRWWICL-----------------RYSF 355
           A+   +  +    + R+I E+D+           +W  L                 ++ F
Sbjct: 194 ALRWAVLTMSRFNYKRIIFESDAQALVNLLNSDDFWPTLQPALEDIQQLLHHFEEVKFEF 253

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150918.13.0e-4229.50uncharacterized protein LOC111018954 [Momordica charantia][more]
XP_042944602.16.9e-3129.80uncharacterized protein LOC122278481 [Carya illinoinensis][more]
TXG71894.14.5e-3029.26hypothetical protein EZV62_000473 [Acer yangbiense][more]
CAB4262994.14.5e-3030.90unnamed protein product [Prunus armeniaca][more]
VVA32947.12.9e-2928.68PREDICTED: retrotransposon [Prunus dulcis][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DAR41.5e-4229.50uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
M5XJH95.2e-3230.61Uncharacterized protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa023013m... [more]
A0A5C7IST22.2e-3029.26RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_000473 ... [more]
A0A6J5TGK72.2e-3030.90CCHC-type domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS... [more]
A0A5E4FZN91.4e-2928.68PREDICTED: retrotransposon OS=Prunus dulcis OX=3755 GN=ALMOND_2B007697 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G34320.12.4e-0525.37Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 248..358
e-value: 2.4E-8
score: 36.1
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 252..323
e-value: 2.0E-11
score: 43.8
NoneNo IPR availablePANTHERPTHR47074:SF37PROTEIN, PUTATIVE-RELATEDcoord: 237..362
NoneNo IPR availablePANTHERPTHR47074BNAC02G40300D PROTEINcoord: 237..362
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 252..354
e-value: 4.41947E-18
score: 77.7396
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 247..358

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0029402.1Lag0029402.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity