Lag0029389 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0029389
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase
Locationchr8: 38491161 .. 38491854 (+)
RNA-Seq ExpressionLag0029389
SyntenyLag0029389
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATAGTTTTTTTAGTTGAAACTAAAAGTTCTAGTCTTATGTCGGACAAGATCAAAAGGAAGTTGGGATTTAATAATGGCATTAGTGTGAGCAGCAGTGGCAACAGTGGAGGGCTGACATTGTTATGGAAAAATCAATATGATATCACAGTGAACTCGGTCTCCAAACGTCATATTGATGTGACCGTAAAGGCTAATGATTGGTGGTGGAGATTCACGGGATTTTACAGGAACCCGGATCAAAACAAGAGAAAAGAATTGTGGCAGCTTATGGAAAGATTAAGTGAAATAGTCAATCTCCAAAGGGACTTCGATGAAATCATGTTTAGCAAAGAAAAGAAAGGAGAAACTTCCAAGAATGTTAGGTATATGAATGATTTCTGTGAAGCTATAAACAAATGTGATTTAATAGATGCACGCTTTTGTGGAGATAGGTTCACTTGGTCAAAAAACAAAGCAAACACCGAAGCAACAAAGGAAAGGCAGGAAACTTTCTAAAATCGCCAATGAGAAACAGCCCCTCTCTGACATGGAGAAGTATCTGTTGGGGACGAGAACTTTGTATGAAAGGATAGGGTGGGCAATGGGAACTACATTGAGATTGGCAATGACCCTTGGATTAACAGATCGGAGAACCCCAAGCCAATCCTCACTAAGGACACTCTCAATGGGCAAAAAGTCTAGAACATTCTGA

mRNA sequence

ATGATAGTTTTTTTAGTTGAAACTAAAAGTTCTAGTCTTATGTCGGACAAGATCAAAAGGAAGTTGGGATTTAATAATGGCATTAGTGTGAGCAGCAGTGGCAACAGTGGAGGGCTGACATTGTTATGGAAAAATCAATATGATATCACAGTGAACTCGGTCTCCAAACGTCATATTGATGTGACCGTAAAGGCTAATGATTGGTGGTGGAGATTCACGGGATTTTACAGGAACCCGGATCAAAACAAGAGAAAAGAATTGTGGCAGCTTATGGAAAGATTAAGTGAAATAGTCAATCTCCAAAGGGACTTCGATGAAATCATGTTTAGCAAAGAAAAGAAAGGAGAAACTTCCAAGAATGTTAGGAAACTTTCTAAAATCGCCAATGAGAAACAGCCCCTCTCTGACATGGAGAAGTATCTGTTGGGGACGAGAACTTTGTATGAAAGGATAGGGTGGGCAATGGGAACTACATTGAGATTGGCAATGACCCTTGGATTAACAGATCGGAGAACCCCAAGCCAATCCTCACTAAGGACACTCTCAATGGGCAAAAAGTCTAGAACATTCTGA

Coding sequence (CDS)

ATGATAGTTTTTTTAGTTGAAACTAAAAGTTCTAGTCTTATGTCGGACAAGATCAAAAGGAAGTTGGGATTTAATAATGGCATTAGTGTGAGCAGCAGTGGCAACAGTGGAGGGCTGACATTGTTATGGAAAAATCAATATGATATCACAGTGAACTCGGTCTCCAAACGTCATATTGATGTGACCGTAAAGGCTAATGATTGGTGGTGGAGATTCACGGGATTTTACAGGAACCCGGATCAAAACAAGAGAAAAGAATTGTGGCAGCTTATGGAAAGATTAAGTGAAATAGTCAATCTCCAAAGGGACTTCGATGAAATCATGTTTAGCAAAGAAAAGAAAGGAGAAACTTCCAAGAATGTTAGGAAACTTTCTAAAATCGCCAATGAGAAACAGCCCCTCTCTGACATGGAGAAGTATCTGTTGGGGACGAGAACTTTGTATGAAAGGATAGGGTGGGCAATGGGAACTACATTGAGATTGGCAATGACCCTTGGATTAACAGATCGGAGAACCCCAAGCCAATCCTCACTAAGGACACTCTCAATGGGCAAAAAGTCTAGAACATTCTGA

Protein sequence

MIVFLVETKSSSLMSDKIKRKLGFNNGISVSSSGNSGGLTLLWKNQYDITVNSVSKRHIDVTVKANDWWWRFTGFYRNPDQNKRKELWQLMERLSEIVNLQRDFDEIMFSKEKKGETSKNVRKLSKIANEKQPLSDMEKYLLGTRTLYERIGWAMGTTLRLAMTLGLTDRRTPSQSSLRTLSMGKKSRTF
Homology
BLAST of Lag0029389 vs. NCBI nr
Match: KAA3478879.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 105.9 bits (263), Expect = 3.9e-19
Identity = 56/125 (44.80%), Postives = 80/125 (64.00%), Query Frame = 0

Query: 2   IVFLVETKSSSLMSDKIKRKLGFNNGISVSSSGNSGGLTLLWKNQYDITVNSVSKRHIDV 61
           +VFL+ETK      +K++RK GF NG  V + G+SGGL L WK +  +T+ S S+ HIDV
Sbjct: 1   MVFLMETKIDKKRMEKVRRKCGFMNGFEVEAEGSSGGLCLAWKEEITVTLRSFSRNHIDV 60

Query: 62  TVKAND--WWWRFTGFYRNPDQNKRKELWQLMERLSEIVNLQRDFDEIMFSKEKKGETSK 121
            VK ++    WRFTGFY +P  N++ + W L+ RL E    QRDF+EI++  EK G   +
Sbjct: 61  MVKEDNVKEEWRFTGFYGSPYSNQKNDTWSLLRRLGED---QRDFNEILYYFEKDGGLLR 120

Query: 122 NVRKL 125
           + RK+
Sbjct: 121 DERKM 122

BLAST of Lag0029389 vs. NCBI nr
Match: KAA3453480.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 103.2 bits (256), Expect = 2.5e-18
Identity = 65/176 (36.93%), Postives = 99/176 (56.25%), Query Frame = 0

Query: 2   IVFLVETKSSSLMSDKIKRKLGFNNGISVSSSGNSGGLTLLWKNQYDITVNSVSKRHIDV 61
           +VFL+ETK  S   D+++R  GF NGI+V + G+ GGL L W++   +T+ S SK HIDV
Sbjct: 31  LVFLMETKLDSKCMDRVRRSCGFTNGINVEAEGSRGGLCLAWRDDIGVTLRSYSKWHIDV 90

Query: 62  TVKAN--DWWWRFTGFYRNPDQNKRKELWQLMERLSEIVN----LQRDFDEIMFSKEKKG 121
            +K       WRFTGFY +P    +K +W L+ERLS+  +    +  DF+EIMFS EK+G
Sbjct: 91  LIKEEGIQEEWRFTGFYGSPYVRDQKYVWNLLERLSQDKDYPWLVAGDFNEIMFSFEKQG 150

Query: 122 ETSKNVRKLSKIANEKQPLSDMEKYLLGTRTLYERIGWAMGTTLRLAMTLGLTDRR 172
              ++ R++    +       M+    GT   +ER G    T +R  +  G+ + +
Sbjct: 151 GQLRDPRRMETFRDTLANCGLMDVGFSGTWFTWER-GNLSETNIRERLDRGVANEK 205

BLAST of Lag0029389 vs. NCBI nr
Match: XP_042944769.1 (uncharacterized protein LOC122278652 [Carya illinoinensis])

HSP 1 Score: 103.2 bits (256), Expect = 2.5e-18
Identity = 53/120 (44.17%), Postives = 78/120 (65.00%), Query Frame = 0

Query: 2   IVFLVETKSSSLMSDKIKRKLGFNNGISVSSSGNSGGLTLLWKNQYDITVNSVSKRHIDV 61
           I+FL ETK SS + D++K ++G +N ++V   G SGGL+LLW+ + ++T+ S SK HID 
Sbjct: 31  IMFLQETKVSSRIMDRVKYQVGLHNCLTVDCEGRSGGLSLLWRQEVELTIKSFSKHHIDA 90

Query: 62  TVKAND--WWWRFTGFYRNPDQNKRKELWQLMERLSEIVN----LQRDFDEIMFSKEKKG 116
            VK +D   WW+ TG YR+P+ ++R+E W LM  LS   +    +  DF+EI    EKKG
Sbjct: 91  EVKDSDNKGWWQVTGLYRHPETSRREETWDLMRSLSRGAHERWLVMGDFNEITSRMEKKG 150

BLAST of Lag0029389 vs. NCBI nr
Match: XP_012468868.1 (PREDICTED: uncharacterized protein LOC105786945 [Gossypium raimondii])

HSP 1 Score: 102.8 bits (255), Expect = 3.3e-18
Identity = 56/129 (43.41%), Postives = 80/129 (62.02%), Query Frame = 0

Query: 2   IVFLVETKSSSLMSDKIKRKLGFNNGISVSSSGNSGGLTLLWKNQYDITVNSVSKRHIDV 61
           +VF +ETK      ++I+R+ G+ NGI V S G  GGL L W+N+ DI++NS SKRHIDV
Sbjct: 77  MVFFMETKLGRQQMERIRRRCGYTNGIEVDSEGTRGGLCLAWRNEVDISLNSFSKRHIDV 136

Query: 62  TV--KANDWWWRFTGFYRNPDQNKRKELWQLMERLSEIVNL----QRDFDEIMFSKEKKG 121
            V  K     WRFTGFY +P    R E W +++ L+  ++L      DF+EIM+  EKKG
Sbjct: 137 LVDDKEIGCKWRFTGFYGSPYTQDRSESWAVLKSLATNMDLPWFVMGDFNEIMYGVEKKG 196

Query: 122 ETSKNVRKL 125
              ++ R++
Sbjct: 197 GLPRDERRM 205

BLAST of Lag0029389 vs. NCBI nr
Match: XP_030963678.1 (uncharacterized protein LOC115984820 [Quercus lobata])

HSP 1 Score: 102.4 bits (254), Expect = 4.3e-18
Identity = 57/120 (47.50%), Postives = 74/120 (61.67%), Query Frame = 0

Query: 2   IVFLVETKSSSLMSDKIKRKLGFNNGISVSSSGNSGGLTLLWKNQYDITVNSVSKRHID- 61
           +VFL+ETK  +   D IKRK+    G+ V S   +GGL LLWKN   + + + S RHID 
Sbjct: 31  LVFLMETKLETTEMDNIKRKVERQQGLVVPSINRAGGLALLWKNSMQVDIQTYSPRHIDA 90

Query: 62  -VTVKANDWWWRFTGFYRNPDQNKRKELWQLMERLSEIVNL----QRDFDEIMFSKEKKG 116
            +T K     WRFTGFY +P+  KR+E W+LME LS   NL      DF+EIM +KEK+G
Sbjct: 91  VITEKQGMKKWRFTGFYEHPETRKREESWKLMENLSHRSNLPWVCMGDFNEIMHAKEKEG 150

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA3478879.13.9e-1944.80reverse transcriptase [Gossypium australe][more]
KAA3453480.12.5e-1836.93reverse transcriptase [Gossypium australe][more]
XP_042944769.12.5e-1844.17uncharacterized protein LOC122278652 [Carya illinoinensis][more]
XP_012468868.13.3e-1843.41PREDICTED: uncharacterized protein LOC105786945 [Gossypium raimondii][more]
XP_030963678.14.3e-1847.50uncharacterized protein LOC115984820 [Quercus lobata][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036691Endonuclease/exonuclease/phosphatase superfamilyGENE3D3.60.10.10Endonuclease/exonuclease/phosphatasecoord: 1..154
e-value: 9.4E-8
score: 34.1
IPR036691Endonuclease/exonuclease/phosphatase superfamilySUPERFAMILY56219DNase I-likecoord: 2..110

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0029389.1Lag0029389.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090304 nucleic acid metabolic process
molecular_function GO:0003824 catalytic activity