Lag0029387 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0029387 vs. NCBI nr
Match: XP_022939278.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.1 putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 992.3 bits (2564), Expect = 1.7e-285
Identity = 481/557 (86.36%), Postives = 520/557 (93.36%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP+PANSSP TP+GFL+RAA VYGD PSIIYNSTTYTW++THRRCLQLASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
           I RG VVSVV+PNT AMYEL FAVPMAGA+LN +NLRLDARTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESS--SSVPISADFIESYEEMVRKGDSEF 180
           SC LIS+ALALFPPE KRPPL+LIAD+AAAE+SS  SSV   ADF++SYEEMV+KGD  F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 181 RWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWTLPIF 240
           +WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIF++ LDSL+EWGVPK+SVYLWTLP+F
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 241 HANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPDNRPL 300
           HANGWS+PWGIAAVGGTNVCIRKFDAA IFALI R+ VTH+CGAPVVLNMLTNSPDNRPL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPATERAR 360
           DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+CCSWK  WNRLPA ERAR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 361 LKARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAKSMTS 420
           LKARQGVRT A +  DV++P TGKSVKRDG SIGEIVLRG S+MLGYLKDPE+TAK+MT+
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
           +GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
           EFWGETPCAFVSL+EGLTR+PTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 541 VLREMAKSMGPGQKSRL 556
           VLREMAKSMGP  KSRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557

BLAST of Lag0029387 vs. NCBI nr
Match: XP_022993747.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 984.6 bits (2544), Expect = 3.6e-283
Identity = 478/561 (85.20%), Postives = 517/561 (92.16%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP+PANSSP TP+GFL+RAA VYGD PSIIYNSTTYTW++THRRCLQLASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
           I RG VVSVV+PNTPAMYEL FAVPMAGA+LN +NLRLD+RTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESS------SSVPISADFIESYEEMVRKG 180
           SC LIS+ALALFPPE KRPPL+LIAD+AAAE+SS      SSV   ADF++SYEEMV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 181 DSEFRWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWT 240
           D  F+WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRGIF++ LDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 241 LPIFHANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPD 300
           LP+FHANGWS+PWGIAAVGGTNVCIRKFDAA IFALI R+ VTH+CGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPAT 360
           NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+CCSWK  WNRLPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 ERARLKARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAK 420
           ERARLKARQGVRT A +  DV+ P TGKSVKRDG SIGEIVLRG S+MLGYLKDPE+TAK
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 SMTSDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480
           +MT++GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540
           ARPDEFWGETPCAFVSL+EGLTR+PTEGEIIEYCRGKLPRFMVPKTVVF AELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 541 IQKFVLREMAKSMGPGQKSRL 556
           IQKFVLREMAKSMGP  +SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of Lag0029387 vs. NCBI nr
Match: XP_023550909.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 984.6 bits (2544), Expect = 3.6e-283
Identity = 478/561 (85.20%), Postives = 519/561 (92.51%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP+PANSSP TP+GFL+RAA VYGD  SIIY+STTYTW++THRRCLQLASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCRSIIYDSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
           I RG VVSVV+PNT AMYEL FAVPMAGA+LN +NLRLDARTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESS------SSVPISADFIESYEEMVRKG 180
           SC LIS+ALALFPPE KRPPL+LIAD+AAAE+SS      SSV   ADF++SYEEMV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVLNRADFVDSYEEMVQKG 180

Query: 181 DSEFRWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWT 240
           D  F+WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIF++ LDSL+EWGVPK+SVYLWT
Sbjct: 181 DPLFQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWT 240

Query: 241 LPIFHANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPD 300
           LP+FHANGWS+PWGIAAVGGTNVCIRKFDAA IFALI R+ VTH+CGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPAT 360
           NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+CCSWK  WNRLPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 ERARLKARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAK 420
           +RARLKARQGVRT A +  DV++P TGKSVKRDG SIGEIVLRG S+MLGYLKDPE+TAK
Sbjct: 361 DRARLKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 SMTSDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480
           +MT++GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540
           ARPDEFWGETPCAFVSL+EGLTR+PTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540

Query: 541 IQKFVLREMAKSMGPGQKSRL 556
           IQKFVLREMAKSMGP  KSRL
Sbjct: 541 IQKFVLREMAKSMGPAGKSRL 561

BLAST of Lag0029387 vs. NCBI nr
Match: XP_038877068.1 (probable acyl-activating enzyme 5, peroxisomal [Benincasa hispida])

HSP 1 Score: 961.8 bits (2485), Expect = 2.5e-276
Identity = 468/558 (83.87%), Postives = 513/558 (91.94%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKPS ANSSP TP+GFL+RA+ +YGDSPSIIYNSTTYTWS+T+RRCL++ASSLSSIG
Sbjct: 28  MEDLKPSLANSSPLTPIGFLERASVIYGDSPSIIYNSTTYTWSETYRRCLRVASSLSSIG 87

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
           I +G+VVSV+APNTP MYELQFAVPMAGA+LN +NLRLDARTISILLRH ESKL+F+DQA
Sbjct: 88  IQKGYVVSVIAPNTPPMYELQFAVPMAGAVLNCINLRLDARTISILLRHCESKLIFIDQA 147

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEE---SSSSVPISADFIESYEEMVRKGDSE 180
           SC LIS+ALALFPPE KRP LVLIADD A E+   SSSS  I  +F++SYEEMV+KGD E
Sbjct: 148 SCGLISDALALFPPETKRPLLVLIADDTAVEDSLSSSSSSVIGGEFVDSYEEMVQKGDDE 207

Query: 181 FRWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWTLPI 240
           FRWIRP SEWDPIV+NYTSGTTSSPKGVVH HRGIF++ LDSL+EWGVPKQSVYLWTLP+
Sbjct: 208 FRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFAVTLDSLIEWGVPKQSVYLWTLPM 267

Query: 241 FHANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPDNRP 300
           FHANGWS+P+GIAAVGGTNVCIRKFDAALIF+LI RH VTH+CGAPVVLNMLTNSPDN+P
Sbjct: 268 FHANGWSFPFGIAAVGGTNVCIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNSPDNQP 327

Query: 301 LDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPATERA 360
           L+RPVKILTAGAPPPAAVLFRTESLGFDV HGYGLTET GLV+ CSWK +WNRLPATERA
Sbjct: 328 LERPVKILTAGAPPPAAVLFRTESLGFDVGHGYGLTETGGLVIYCSWKSKWNRLPATERA 387

Query: 361 RLKARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAKSMT 420
           RLKARQGVRT A S VDV++  TGKSVKRDGASIGEIVLRGGS+MLGY KDPE+T K+MT
Sbjct: 388 RLKARQGVRTLAMSEVDVLDSETGKSVKRDGASIGEIVLRGGSMMLGYFKDPEATKKAMT 447

Query: 421 SDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 480
            +GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP
Sbjct: 448 DEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 507

Query: 481 DEFWGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQK 540
           DEFWGETPCAFVSLREGLTR PTE EI+EYCR KLP+FMVPKTVVF  +LPKTSTGKIQK
Sbjct: 508 DEFWGETPCAFVSLREGLTRLPTEEEIMEYCREKLPKFMVPKTVVFMEKLPKTSTGKIQK 567

Query: 541 FVLREMAKSMGPGQKSRL 556
           F+LRE AKSMGP + SRL
Sbjct: 568 FLLREKAKSMGPARTSRL 585

BLAST of Lag0029387 vs. NCBI nr
Match: XP_008463174.1 (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA0046220.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa] >TYK18976.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 954.5 bits (2466), Expect = 4.0e-274
Identity = 458/557 (82.23%), Postives = 507/557 (91.02%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP+PANS P TP+GF+ RAA +YGDS SIIYNSTTYTWS+THRRCLQLASS+SS G
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
           I RGHVVSV+APNTPAMYEL FAVPM GAILN +NLRLD RTISILLRHSESKL+FVDQA
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESS--SSVPISADFIESYEEMVRKGDSEF 180
           SCALI EALALFPPE KRP LVLIADDAA ++SS  S V I  +F++SYE+MV++GDS+F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 RWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWTLPIF 240
           +WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRGIF++ LDSLLEWGVPKQSVYLWTLP+F
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPDNRPL 300
           H NGWS+PWGIAAVGGTNVCIRKFDA LIF+LIRRH VTH+CGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPATERAR 360
           DRPVKILTAGAPPPA++L RTESLGF V+HGYGLTE+AG ++CCSWK +WN+LPA E+AR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAKSMTS 420
           LKARQGVR  A  AVDV++P T KSVKRDGASIGEIV+RG S+ LGYLKDPE TAK+M+ 
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
           EFWGETPCAF+SL+EGL+RKPTE EIIEYC+GKLPRFMVPKTV+F  E+PKT+TGKIQKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLREMAKSMGPGQKSRL 556
           VLR+ AKS+GP  KSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of Lag0029387 vs. ExPASy Swiss-Prot
Match: M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)

HSP 1 Score: 860.1 bits (2221), Expect = 1.3e-248
Identity = 404/548 (73.72%), Postives = 480/548 (87.59%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP PA+SSP TPLGFL+RAA VYGD  S++Y++ +YTWSQTHRRCL LASS++S+G
Sbjct: 1   MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
           I  GHVVSV+APN P MYEL FAVPMAGAILN VNLRLDARTISILL HSESKL+FVD  
Sbjct: 61  IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIESYEEMVRKGDSEFRW 180
           S  LI EA+ALFP +A  P LV +AD++   ES +S  +  +F  SY++++ +GD +F+W
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMADES---ESGNSSELGKEFFCSYKDLIDRGDPDFKW 180

Query: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWTLPIFHA 240
           + P SEWDP++LNYTSGTTSSPKGVVHCHRGIF + +DSL++WGVPKQ VYLWTLP+FHA
Sbjct: 181 VMPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMFHA 240

Query: 241 NGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPDNRPLDR 300
           NGWSYPWG+AAVGGTN+C+RKFD+ +I+ +I+RH VTHMCGAPVVLNML+N+P + PL  
Sbjct: 241 NGWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPLKT 300

Query: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPATERARLK 360
            V+I+TAGAPPP+AVLFRTESLGF VSHGYGLTETAGLVV C+WK +WN LPATERARLK
Sbjct: 301 TVQIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERARLK 360

Query: 361 ARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAKSMTSDG 420
           +RQGV T   + +DVV+P TG +VKRDG+++GE+VLRGGS+MLGYLKDPE TAKSMT+DG
Sbjct: 361 SRQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTADG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480
           WF+TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY++P + EAAVVARPDEF
Sbjct: 421 WFYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPDEF 480

Query: 481 WGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKFVL 540
           WGETPCAFVSL++GLT+KPTE EI+EYCR KLPR+MVPKTVVF  ELPKTSTGK+QKF+L
Sbjct: 481 WGETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKFIL 540

Query: 541 REMAKSMG 549
           R+MA+ MG
Sbjct: 541 RDMARGMG 545

BLAST of Lag0029387 vs. ExPASy Swiss-Prot
Match: Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 8.2e-230
Identity = 383/556 (68.88%), Postives = 462/556 (83.09%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNS-TTYTWSQTHRRCLQLASSLSSI 60
           ME +KP  ANS P TP+GFL+RAA VYGD  SI+Y S T YTW +T+ RCL++ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQ 120
           GI R  VVSV++PNTPAMYELQFAVPM+GAILNN+N RLDART+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 ASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIESYEEMVRKGDSEFR 180
            S  L  EA+++   +   PP+++   D   E   + V     F  +Y++++ +GD +F+
Sbjct: 121 FSVDLAVEAISMMTTD---PPILVFIADKEEEGGDADVADRTKFSYTYDDLIHRGDLDFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWTLPIFH 240
           WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF + +DSL++W VPK  VYLWTLPIFH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFH 240

Query: 241 ANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPDNRPLD 300
           ANGWSYPWGIAAVGGTNVC+RKFDA LI+ LIR H VTHMCGAPVVLNML+ + + +PL+
Sbjct: 241 ANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPLN 300

Query: 301 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPATERARL 360
           RPV ILTAGAPPPAAVL R ES+GF +SHGYGLTETAGL V C+WKPQWNRLPA++RARL
Sbjct: 301 RPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRARL 360

Query: 361 KARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAKSMTSD 420
           KARQGVRT  F+ +DVV+P +G+SV+R+G ++GEIV+RG SIMLGYLKDP  T K++  +
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL-KN 420

Query: 421 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 480
           GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNPAVNE AVVARPD 
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPDV 480

Query: 481 FWGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKFV 540
           FWGETPCAFVSL+ GLT++PTE E+IEYCR K+P++MVPKTV F  ELPKTSTGK+ KFV
Sbjct: 481 FWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKFV 540

Query: 541 LREMAKSMGPGQKSRL 556
           LRE+AK MG  + SR+
Sbjct: 541 LREIAKKMGTTRLSRM 552

BLAST of Lag0029387 vs. ExPASy Swiss-Prot
Match: M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 1.2e-225
Identity = 378/555 (68.11%), Postives = 455/555 (81.98%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSS-I 60
           ME+LKP  ANS P TPLGFL+RAA VYGD  S++Y+  +YTWSQTH RCL++AS + S +
Sbjct: 1   MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60

Query: 61  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQ 120
           G+ +G VVSVVAPN PAMYEL FAVPMAG +LNN+N RL+A TIS++LRHSESKLVFVDQ
Sbjct: 61  GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120

Query: 121 ASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIESYEEMVRKGDSE-F 180
            S  L+ +A++LFP     P LVLIAD+   E  + +V     F+  YE++V KGD + F
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIADNITGE--NLTVEDREHFVCCYEDLVEKGDDKTF 180

Query: 181 RWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWTLPIF 240
           +W+RP SEWDPIVLNYTSGTTSSPKGVVH HR +F + LDSL++WGVPKQ VYLWTLP+F
Sbjct: 181 KWVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLPMF 240

Query: 241 HANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPDNRPL 300
           HANGW Y WGIAAVGGTN+C+R+FD  +IF LIRRHRVTHMC AP+VLNML+NSP+  PL
Sbjct: 241 HANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAEPL 300

Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPATERAR 360
             PV ++T GAPPPAAVL RTESLGF +SHGYG+TE  G+VV C+WK +WNRLPATE+AR
Sbjct: 301 PNPVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQAR 360

Query: 361 LKARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAKSMTS 420
           LK+RQGVRTAA   VDVV+P +G SVKRDG ++GEIVL+G SIMLGYLK+  +TAK + +
Sbjct: 361 LKSRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCIRA 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
           DGWF+TGD+ VMHPDGYLEIKDRSKDVIISGGEN+SSVEVES LY++PAV+EAAVVA PD
Sbjct: 421 DGWFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVACPD 480

Query: 481 EFWGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
           E+WGETP AFV+L++G+  +PTE EI+EYCR KL  FMVPK VVF  +LPKTSTGKIQKF
Sbjct: 481 EYWGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQKF 540

Query: 541 VLREMAKSMGPGQKS 554
            L+E+ K+MG    S
Sbjct: 541 TLKEIVKTMGYSSSS 553

BLAST of Lag0029387 vs. ExPASy Swiss-Prot
Match: Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 1.1e-223
Identity = 368/549 (67.03%), Postives = 456/549 (83.06%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNS-TTYTWSQTHRRCLQLASSLSSI 60
           ME++KP  ANS P TP+GFL+RAA VYGD  SI+Y S T YTW +T+ RCL++ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQ 120
           GI R  VVSV++PNTPAMYELQFAVPM+GAILNN+N RLDART+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 ASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIESYEEMVRKGDSEFR 180
            S  L  EA+++   +   PP++++  D   E   + V   + F  +Y++++ +GD  F+
Sbjct: 121 FSVDLAVEAVSMMTTD---PPILVVIADKEEEGGVADVADLSKFSYTYDDLIERGDPGFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWTLPIFH 240
           WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF + +DSL++W VPK  VYLWTLPIFH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIFH 240

Query: 241 ANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPDNRPLD 300
           +NGW+ PWGIAAVGGTNVC+RKFDA LI+ LIR H VTHMCGAPVVLNML+ + +++PL+
Sbjct: 241 SNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPLN 300

Query: 301 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPATERARL 360
            PV ILTAG+PPPA VL R ES+GF +SHGYGLTETAG++V C+WKP+WN LPA++RARL
Sbjct: 301 HPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRARL 360

Query: 361 KARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAKSMTSD 420
           KARQGVRT  F+ +DVV+P +G SV+R+G ++GEIV+RG S+MLGYLKDP  T K++  +
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL-KN 420

Query: 421 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 480
           GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT PAVNE AVVARPDE
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPDE 480

Query: 481 FWGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKFV 540
           FWGETPCAFVSL+ G + KPTE E++EYCR K+P++MVPKTV F  ELPK+STGK+ KFV
Sbjct: 481 FWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKFV 540

Query: 541 LREMAKSMG 549
           LR++AK MG
Sbjct: 541 LRDIAKKMG 545

BLAST of Lag0029387 vs. ExPASy Swiss-Prot
Match: Q9LQS1 (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 6.1e-217
Identity = 371/549 (67.58%), Postives = 444/549 (80.87%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIY-NSTTYTWSQTHRRCLQLASSLSSI 60
           MEDLKPS ANS P T LGFL+RAA VYGD  SI+Y NST YTW +T+ RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQ 120
           GI R  VVSV++ NTP MYELQF+VPM+GAILNN+N RLDART+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 ASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIESYEEMVRKGDSEFR 180
               L  EA+ +       PP++++  +   EE  + V   + F   Y +++ +G+ +F+
Sbjct: 121 FYSDLAVEAITML----LNPPILVLIANEEEEEGGAEVTERSKFCYLYSDLITRGNPDFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWTLPIFH 240
           WIRP SEWDPIV+NYTSGTTSSPKGVVHCHRGIF + LDSL +W VPK  VYLWTLPIFH
Sbjct: 181 WIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFH 240

Query: 241 ANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPD-NRPL 300
           ANGW+YPWGIAAVGGTNVC+RK  A  I+ LIR H VTHM GAP+VL +L+ S + ++PL
Sbjct: 241 ANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPL 300

Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPATERAR 360
             PV  LTAG+ PPA VL R ESLGF VSHGYGLTETAG++V C+WKP WNRLPA+++A+
Sbjct: 301 KSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQ 360

Query: 361 LKARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAKSMTS 420
           LK+RQGVRT  FS +DVV+P +G+SV+RDG ++GEIVLRG SIMLGYLK+P  T  S   
Sbjct: 361 LKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF-K 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
           +GWFFTGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNPAVNEAAVVARPD
Sbjct: 421 NGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
           EFWGETPCAFVSL+ GLTRKPT+ EIIEYC+ K+PR+M PKTV F  ELPKTSTGKI K 
Sbjct: 481 EFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKS 540

Query: 541 VLREMAKSM 548
           +L+E+AK+M
Sbjct: 541 LLKEIAKNM 544

BLAST of Lag0029387 vs. ExPASy TrEMBL
Match: A0A6J1FL76 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111445243 PE=4 SV=1)

HSP 1 Score: 992.3 bits (2564), Expect = 8.3e-286
Identity = 481/557 (86.36%), Postives = 520/557 (93.36%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP+PANSSP TP+GFL+RAA VYGD PSIIYNSTTYTW++THRRCLQLASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
           I RG VVSVV+PNT AMYEL FAVPMAGA+LN +NLRLDARTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESS--SSVPISADFIESYEEMVRKGDSEF 180
           SC LIS+ALALFPPE KRPPL+LIAD+AAAE+SS  SSV   ADF++SYEEMV+KGD  F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 181 RWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWTLPIF 240
           +WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIF++ LDSL+EWGVPK+SVYLWTLP+F
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 241 HANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPDNRPL 300
           HANGWS+PWGIAAVGGTNVCIRKFDAA IFALI R+ VTH+CGAPVVLNMLTNSPDNRPL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPATERAR 360
           DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+CCSWK  WNRLPA ERAR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 361 LKARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAKSMTS 420
           LKARQGVRT A +  DV++P TGKSVKRDG SIGEIVLRG S+MLGYLKDPE+TAK+MT+
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
           +GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
           EFWGETPCAFVSL+EGLTR+PTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 541 VLREMAKSMGPGQKSRL 556
           VLREMAKSMGP  KSRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557

BLAST of Lag0029387 vs. ExPASy TrEMBL
Match: A0A6J1JTQ2 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111489657 PE=4 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 1.7e-283
Identity = 478/561 (85.20%), Postives = 517/561 (92.16%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP+PANSSP TP+GFL+RAA VYGD PSIIYNSTTYTW++THRRCLQLASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
           I RG VVSVV+PNTPAMYEL FAVPMAGA+LN +NLRLD+RTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESS------SSVPISADFIESYEEMVRKG 180
           SC LIS+ALALFPPE KRPPL+LIAD+AAAE+SS      SSV   ADF++SYEEMV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 181 DSEFRWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWT 240
           D  F+WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRGIF++ LDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 241 LPIFHANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPD 300
           LP+FHANGWS+PWGIAAVGGTNVCIRKFDAA IFALI R+ VTH+CGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPAT 360
           NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+CCSWK  WNRLPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 ERARLKARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAK 420
           ERARLKARQGVRT A +  DV+ P TGKSVKRDG SIGEIVLRG S+MLGYLKDPE+TAK
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 SMTSDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480
           +MT++GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540
           ARPDEFWGETPCAFVSL+EGLTR+PTEGEIIEYCRGKLPRFMVPKTVVF AELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 541 IQKFVLREMAKSMGPGQKSRL 556
           IQKFVLREMAKSMGP  +SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of Lag0029387 vs. ExPASy TrEMBL
Match: A0A5D3D623 (Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold418G00690 PE=4 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 1.9e-274
Identity = 458/557 (82.23%), Postives = 507/557 (91.02%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP+PANS P TP+GF+ RAA +YGDS SIIYNSTTYTWS+THRRCLQLASS+SS G
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
           I RGHVVSV+APNTPAMYEL FAVPM GAILN +NLRLD RTISILLRHSESKL+FVDQA
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESS--SSVPISADFIESYEEMVRKGDSEF 180
           SCALI EALALFPPE KRP LVLIADDAA ++SS  S V I  +F++SYE+MV++GDS+F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 RWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWTLPIF 240
           +WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRGIF++ LDSLLEWGVPKQSVYLWTLP+F
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPDNRPL 300
           H NGWS+PWGIAAVGGTNVCIRKFDA LIF+LIRRH VTH+CGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPATERAR 360
           DRPVKILTAGAPPPA++L RTESLGF V+HGYGLTE+AG ++CCSWK +WN+LPA E+AR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAKSMTS 420
           LKARQGVR  A  AVDV++P T KSVKRDGASIGEIV+RG S+ LGYLKDPE TAK+M+ 
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
           EFWGETPCAF+SL+EGL+RKPTE EIIEYC+GKLPRFMVPKTV+F  E+PKT+TGKIQKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLREMAKSMGPGQKSRL 556
           VLR+ AKS+GP  KSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of Lag0029387 vs. ExPASy TrEMBL
Match: A0A1S3CIL0 (probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103501381 PE=4 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 1.9e-274
Identity = 458/557 (82.23%), Postives = 507/557 (91.02%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP+PANS P TP+GF+ RAA +YGDS SIIYNSTTYTWS+THRRCLQLASS+SS G
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
           I RGHVVSV+APNTPAMYEL FAVPM GAILN +NLRLD RTISILLRHSESKL+FVDQA
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESS--SSVPISADFIESYEEMVRKGDSEF 180
           SCALI EALALFPPE KRP LVLIADDAA ++SS  S V I  +F++SYE+MV++GDS+F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 RWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWTLPIF 240
           +WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRGIF++ LDSLLEWGVPKQSVYLWTLP+F
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPDNRPL 300
           H NGWS+PWGIAAVGGTNVCIRKFDA LIF+LIRRH VTH+CGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPATERAR 360
           DRPVKILTAGAPPPA++L RTESLGF V+HGYGLTE+AG ++CCSWK +WN+LPA E+AR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAKSMTS 420
           LKARQGVR  A  AVDV++P T KSVKRDGASIGEIV+RG S+ LGYLKDPE TAK+M+ 
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
           EFWGETPCAF+SL+EGL+RKPTE EIIEYC+GKLPRFMVPKTV+F  E+PKT+TGKIQKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLREMAKSMGPGQKSRL 556
           VLR+ AKS+GP  KSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of Lag0029387 vs. ExPASy TrEMBL
Match: A0A5A7TXV0 (Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold418G00770 PE=4 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 2.4e-272
Identity = 457/555 (82.34%), Postives = 510/555 (91.89%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           M+ LKP+PANSSP TP+ FL+RA+ VY DSPSIIYNST++TWSQT+ RCL+LASSLSS+G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
           IH+GHVVSV++PNTP MYELQF+VPMAGAIL+ +NLRLDARTIS+ LRHSESKL+FVDQA
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIESYEEMVRKGDSEFRW 180
           S ALIS+AL LFPPE+KRP LVLIADDAA E+S+SSV I  DF++ YE+M++KGD EFRW
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFVDFYEDMIQKGDPEFRW 180

Query: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWTLPIFHA 240
           IRP SEWDPIV+NYTSGTTSSPKGVVH HRGIF++ LDSL+EWGVPK SVYLWTLP+FHA
Sbjct: 181 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 240

Query: 241 NGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPDNRPLDR 300
           NGWS+P+ IAAVGGTN+CIRKFDAALIF+LI RH VTH+CGAPVVLNMLTNSP+NRPL+R
Sbjct: 241 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLER 300

Query: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPATERARLK 360
           PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK +WN+LPATERARLK
Sbjct: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARLK 360

Query: 361 ARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAKSMTSDG 420
           ARQGVRT A +  DV++  TGKSVKRDG SIGEIVLRGGS+MLGY KDPE+T K+MT +G
Sbjct: 361 ARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEEG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480
           WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480

Query: 481 WGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKFVL 540
           WGETPCAFVSLREGL+R PTE EIIEYCRGKL  FMVPKTVVF  +LPKTSTGKIQKF+L
Sbjct: 481 WGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 540

Query: 541 REMAKSMGPGQKSRL 556
           REMAKSMGP +KSRL
Sbjct: 541 REMAKSMGPSRKSRL 555

BLAST of Lag0029387 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 797.7 bits (2059), Expect = 5.8e-231
Identity = 383/556 (68.88%), Postives = 462/556 (83.09%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNS-TTYTWSQTHRRCLQLASSLSSI 60
           ME +KP  ANS P TP+GFL+RAA VYGD  SI+Y S T YTW +T+ RCL++ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQ 120
           GI R  VVSV++PNTPAMYELQFAVPM+GAILNN+N RLDART+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 ASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIESYEEMVRKGDSEFR 180
            S  L  EA+++   +   PP+++   D   E   + V     F  +Y++++ +GD +F+
Sbjct: 121 FSVDLAVEAISMMTTD---PPILVFIADKEEEGGDADVADRTKFSYTYDDLIHRGDLDFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWTLPIFH 240
           WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF + +DSL++W VPK  VYLWTLPIFH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFH 240

Query: 241 ANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPDNRPLD 300
           ANGWSYPWGIAAVGGTNVC+RKFDA LI+ LIR H VTHMCGAPVVLNML+ + + +PL+
Sbjct: 241 ANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPLN 300

Query: 301 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPATERARL 360
           RPV ILTAGAPPPAAVL R ES+GF +SHGYGLTETAGL V C+WKPQWNRLPA++RARL
Sbjct: 301 RPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRARL 360

Query: 361 KARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAKSMTSD 420
           KARQGVRT  F+ +DVV+P +G+SV+R+G ++GEIV+RG SIMLGYLKDP  T K++  +
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL-KN 420

Query: 421 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 480
           GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNPAVNE AVVARPD 
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPDV 480

Query: 481 FWGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKFV 540
           FWGETPCAFVSL+ GLT++PTE E+IEYCR K+P++MVPKTV F  ELPKTSTGK+ KFV
Sbjct: 481 FWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKFV 540

Query: 541 LREMAKSMGPGQKSRL 556
           LRE+AK MG  + SR+
Sbjct: 541 LREIAKKMGTTRLSRM 552

BLAST of Lag0029387 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 777.3 bits (2006), Expect = 8.1e-225
Identity = 368/549 (67.03%), Postives = 456/549 (83.06%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNS-TTYTWSQTHRRCLQLASSLSSI 60
           ME++KP  ANS P TP+GFL+RAA VYGD  SI+Y S T YTW +T+ RCL++ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQ 120
           GI R  VVSV++PNTPAMYELQFAVPM+GAILNN+N RLDART+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 ASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIESYEEMVRKGDSEFR 180
            S  L  EA+++   +   PP++++  D   E   + V   + F  +Y++++ +GD  F+
Sbjct: 121 FSVDLAVEAVSMMTTD---PPILVVIADKEEEGGVADVADLSKFSYTYDDLIERGDPGFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWTLPIFH 240
           WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF + +DSL++W VPK  VYLWTLPIFH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIFH 240

Query: 241 ANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPDNRPLD 300
           +NGW+ PWGIAAVGGTNVC+RKFDA LI+ LIR H VTHMCGAPVVLNML+ + +++PL+
Sbjct: 241 SNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPLN 300

Query: 301 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPATERARL 360
            PV ILTAG+PPPA VL R ES+GF +SHGYGLTETAG++V C+WKP+WN LPA++RARL
Sbjct: 301 HPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRARL 360

Query: 361 KARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAKSMTSD 420
           KARQGVRT  F+ +DVV+P +G SV+R+G ++GEIV+RG S+MLGYLKDP  T K++  +
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL-KN 420

Query: 421 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 480
           GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT PAVNE AVVARPDE
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPDE 480

Query: 481 FWGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKFV 540
           FWGETPCAFVSL+ G + KPTE E++EYCR K+P++MVPKTV F  ELPK+STGK+ KFV
Sbjct: 481 FWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKFV 540

Query: 541 LREMAKSMG 549
           LR++AK MG
Sbjct: 541 LRDIAKKMG 545

BLAST of Lag0029387 vs. TAIR 10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 755.0 bits (1948), Expect = 4.3e-218
Identity = 371/549 (67.58%), Postives = 444/549 (80.87%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIY-NSTTYTWSQTHRRCLQLASSLSSI 60
           MEDLKPS ANS P T LGFL+RAA VYGD  SI+Y NST YTW +T+ RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQ 120
           GI R  VVSV++ NTP MYELQF+VPM+GAILNN+N RLDART+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 ASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIESYEEMVRKGDSEFR 180
               L  EA+ +       PP++++  +   EE  + V   + F   Y +++ +G+ +F+
Sbjct: 121 FYSDLAVEAITML----LNPPILVLIANEEEEEGGAEVTERSKFCYLYSDLITRGNPDFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWTLPIFH 240
           WIRP SEWDPIV+NYTSGTTSSPKGVVHCHRGIF + LDSL +W VPK  VYLWTLPIFH
Sbjct: 181 WIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFH 240

Query: 241 ANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPD-NRPL 300
           ANGW+YPWGIAAVGGTNVC+RK  A  I+ LIR H VTHM GAP+VL +L+ S + ++PL
Sbjct: 241 ANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPL 300

Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPATERAR 360
             PV  LTAG+ PPA VL R ESLGF VSHGYGLTETAG++V C+WKP WNRLPA+++A+
Sbjct: 301 KSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQ 360

Query: 361 LKARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAKSMTS 420
           LK+RQGVRT  FS +DVV+P +G+SV+RDG ++GEIVLRG SIMLGYLK+P  T  S   
Sbjct: 361 LKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF-K 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
           +GWFFTGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNPAVNEAAVVARPD
Sbjct: 421 NGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
           EFWGETPCAFVSL+ GLTRKPT+ EIIEYC+ K+PR+M PKTV F  ELPKTSTGKI K 
Sbjct: 481 EFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKS 540

Query: 541 VLREMAKSM 548
           +L+E+AK+M
Sbjct: 541 LLKEIAKNM 544

BLAST of Lag0029387 vs. TAIR 10
Match: AT1G77240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 733.0 bits (1891), Expect = 1.8e-211
Identity = 348/549 (63.39%), Postives = 440/549 (80.15%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLS--S 60
           ME L P  +NS P T LGFL+RAA+V+GDSPS+++ +T +TWS+TH RCL++AS+LS  S
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 61  IGIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVD 120
           +GI+RG VVSV+ PN P++YELQFAVPM+GA+LNN+N RLDA  +S+LLRHSESKLVFVD
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 121 QASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIESYEEMVRKGDSEF 180
             S +L+ EA++ F P+ +RP LV++ D      SSS+     DF+++YE  + +GD  F
Sbjct: 121 HHSSSLVLEAVS-FLPKDERPRLVILNDGNDMPSSSSA---DMDFLDTYEGFMERGDLRF 180

Query: 181 RWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWTLPIF 240
           +W+RP SEW P+VLNYTSGTTSSPKGVVH HR +F   ++SLL+W +P + VYLWTLP+F
Sbjct: 181 KWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLPMF 240

Query: 241 HANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPDNRPL 300
           HANGWSY W  AAVG  N+C+ + D   IF LI +++VTHMC AP+VLNMLTN P  +PL
Sbjct: 241 HANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQKPL 300

Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPATERAR 360
             PVK++TAGAPPPA V+ + E+LGFDVSHGYG+TET GLVV C+ KP+W+RL   ERA+
Sbjct: 301 QSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDERAK 360

Query: 361 LKARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAKSMTS 420
            K+RQG+RTA F+ VDV +P +GKSVK DGA++GEIV RGGS+MLGY KDPE TA SM  
Sbjct: 361 QKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASMRE 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
           DGWF+TGD+GVMHPDGYLE+KDRSKDV+I GGEN+SS E+E+VLYTNPA+ EAAVVA+PD
Sbjct: 421 DGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAKPD 480

Query: 481 EFWGETPCAFVSLREGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
           + WGETPCAFVSL+       TE EI E+C+ KLP++MVP+ VVF  ELPKTSTGKIQKF
Sbjct: 481 KMWGETPCAFVSLKYH-DGSVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQKF 540

Query: 541 VLREMAKSM 548
           +LR+MAKS+
Sbjct: 541 LLRQMAKSL 544

BLAST of Lag0029387 vs. TAIR 10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 727.2 bits (1876), Expect = 9.6e-210
Identity = 353/555 (63.60%), Postives = 441/555 (79.46%), Query Frame = 0

Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIY-NSTTYTWSQTHRRCLQLASSL--S 60
           ME L P P+NS+P T LGFLDRAA+VYGD PSI++  +T +TWS+TH RCL++AS+L  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  SIGIHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFV 120
           S+GI+RG VVSVV PN P++YELQFAVPM+GAILNN+N RLDA  +S+LLRHSESKLVFV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DQASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIESYEEMVRKGDSE 180
           D  S +++ EA++ F  + ++P LVL+ DD   E+ S S   ++DF+++Y+ ++ +GDS 
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDD--QEDGSLSPSAASDFLDTYQGVMERGDSR 180

Query: 181 FRWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFSIILDSLLEWGVPKQSVYLWTLPI 240
           F+WIRP +EW P++LNYTSGTTSSPKGVV  HR IF + + SLL+W  P + VYLWTLP+
Sbjct: 181 FKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTLPM 240

Query: 241 FHANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSPDNRP 300
           FHANGW Y WG AAVG TNVC R+ DA  I+ LI +H VTHMC AP+VLNMLTN P  +P
Sbjct: 241 FHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSRKP 300

Query: 301 LDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVCCSWKPQWNRLPATERA 360
           L  PV+++TAGAPPPAA++ R E+LGF+V HGYGLTET G VV C+WK +W+ L   ERA
Sbjct: 301 LKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLERA 360

Query: 361 RLKARQGVRTAAFSAVDVVEPATGKSVKRDGASIGEIVLRGGSIMLGYLKDPESTAKSMT 420
           RLK+RQGVRT  F+ VDV +P TGKSV+ DG S+GEIVL+GGS+MLGY KDPE TA  M 
Sbjct: 361 RLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAACMR 420

Query: 421 SDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 480
            DGWF++GDVGV+H DGYLE+KDRSKDVII GGEN+SS EVE+VLYTNP V EAAVVA+P
Sbjct: 421 EDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVAKP 480

Query: 481 DEFWGETPCAFVSLR-----EGLTRKPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTST 540
           D+ WGETPCAFVSL+      GL    TE EI E+C+ +LP++MVP+ V+F  ELPKTST
Sbjct: 481 DKMWGETPCAFVSLKYDSNGNGLV---TEREIREFCKTRLPKYMVPRKVIFQEELPKTST 540

Query: 541 GKIQKFVLREMAKSM 548
           GKIQKF+LR+MAKS+
Sbjct: 541 GKIQKFLLRQMAKSL 549