Lag0029358 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0029358
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	15.7 kDa heat shock protein, peroxisomal
Location	chr8: 38151381 .. 38152837 (-)
RNA-Seq Expression	Lag0029358
Synteny	Lag0029358

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAAGGACGAAGAGAAGAAGTCTCTGGAGATCATTTCAAATTCCACAGACTTTCAGCTGCAATCTTCAATCTGTGCCTTTTGTGAAGCTTCGATCCCTTAGCTTGCTTCCAATGGCGGATGATTTATTTGGATATCCTCTCCGGCGTTTCTTCTGGAGCCCTGCCGTCTTCCGAGGACCGTCCGCCGCCGTTGCGCTCTTGGATTGGCTCGAAACATCCAATGCTCACATCTTCAAGGTTAACGTTCCAGGTACCTTTTCGTTATCGCTTGGCTTTGAATGCTGATTTACGCAATACGTTCCTTGATTTGAGGTATGAGAAATTTCTGTATGTAATGCCTCTGCGGAGTGGAGATTCTCTAGGTTTTGTGGATTGAGTTTCTTGTTTAATTAGTTTTTCTTGTCTAAGCTTCTTTTCTTTTGCTTGGATTCTTAAACTTGTTCGGTTCATTCGGAGTTATTTTAAGGAACTTATTCACTCGGTTCGGTTTGAGGAATCGTTGTATTGTTTCTACTTTAGATTTGATGCGAAATCAAGCAATTAGTTTAATGAGTTTGAGATTTGGAAAGTGTTTTTTGTCTTGGATAATTGAGTCGATCGTCGCCTTTGAAACTTTTTGAAAAGAATGAAAATTAAACACGGTTTAATTTTAGACAAACATGGTATTTAATTGAGTGTTTATGCATTTAGTTTCTTACCGTGGATTAAGATGTGGAGAATAGAATTGTAAGAAAGTGACAAACTGAATCGTATTTTTTTTTTAAAAGAAACTTTAACTGAATCGTATTTTGTGAAGTGACTGTCATTTGATTTGTTGATAAATTAATTATGGTGGACACAAAATACGTGGGGAAAAAATAATCTAAGTCCAGGGGAAACGAGTTCAAAACTATAGTGGTCAGTGGTCACCGGCCAAATGCCATCTATTTAGGATTTAAAATCTCATGAATTTTCTTAGCAACTACTAGCTGATTAGTCAAAGTGTATATGAGTAGTTCAAATATTCACGGATATCAACAATAAAATATTATATAGAAAAAAAATAAAAATCACTTTGTCTCTTCACAAAATACAAATTTAGGTCACTACTCTTAAATTCTCTCTGTTTTTTTCTTCCCAGGTTAGCTATTTGATAGATTATTTGGTTGTTGCTATTTGAAATCTGTTTAGGCTTTAGCAAAGATGACATAAAGGTACAGGTTGATGAGGGAAATGTTCTGCACATCAAAGGAAACAATAAAGAGGGACCTCTTGGGAAAGAGGCCATTTGGCATTTGGCTGAAAGACAAACAGGGAAGAGAGATTTTTCTAGGGAGATTGAACTGCCTGAAAATGTCAAATTAGACCAGATTAAAGCTCAGGTTGAAAATGGTCTCCTCACCATTGTTGTCCCAAAAGATACAGCTCCAAGGTCATCAAAGGTCAGGAACATCAACATTACGAGTAAGCTTTGA

mRNA sequence

ATGATAAGGACGAAGAGAAGAAGTCTCTGGAGATCATTTCAAATTCCACAGACTTTCAGCTGCAATCTTCAATCTGTGCCTTTTGTGAAGCTTCGATCCCTTAGCTTGCTTCCAATGGCGGATGATTTATTTGGATATCCTCTCCGGCGTTTCTTCTGGAGCCCTGCCGTCTTCCGAGGACCGTCCGCCGCCGTTGCGCTCTTGGATTGGCTCGAAACATCCAATGCTCACATCTTCAAGGTTAACGTTCCAGGCTTTAGCAAAGATGACATAAAGGTACAGGTTGATGAGGGAAATGTTCTGCACATCAAAGGAAACAATAAAGAGGGACCTCTTGGGAAAGAGGCCATTTGGCATTTGGCTGAAAGACAAACAGGGAAGAGAGATTTTTCTAGGGAGATTGAACTGCCTGAAAATGTCAAATTAGACCAGATTAAAGCTCAGGTTGAAAATGGTCTCCTCACCATTGTTGTCCCAAAAGATACAGCTCCAAGGTCATCAAAGGTCAGGAACATCAACATTACGAGTAAGCTTTGA

Coding sequence (CDS)

ATGATAAGGACGAAGAGAAGAAGTCTCTGGAGATCATTTCAAATTCCACAGACTTTCAGCTGCAATCTTCAATCTGTGCCTTTTGTGAAGCTTCGATCCCTTAGCTTGCTTCCAATGGCGGATGATTTATTTGGATATCCTCTCCGGCGTTTCTTCTGGAGCCCTGCCGTCTTCCGAGGACCGTCCGCCGCCGTTGCGCTCTTGGATTGGCTCGAAACATCCAATGCTCACATCTTCAAGGTTAACGTTCCAGGCTTTAGCAAAGATGACATAAAGGTACAGGTTGATGAGGGAAATGTTCTGCACATCAAAGGAAACAATAAAGAGGGACCTCTTGGGAAAGAGGCCATTTGGCATTTGGCTGAAAGACAAACAGGGAAGAGAGATTTTTCTAGGGAGATTGAACTGCCTGAAAATGTCAAATTAGACCAGATTAAAGCTCAGGTTGAAAATGGTCTCCTCACCATTGTTGTCCCAAAAGATACAGCTCCAAGGTCATCAAAGGTCAGGAACATCAACATTACGAGTAAGCTTTGA

Protein sequence

MIRTKRRSLWRSFQIPQTFSCNLQSVPFVKLRSLSLLPMADDLFGYPLRRFFWSPAVFRGPSAAVALLDWLETSNAHIFKVNVPGFSKDDIKVQVDEGNVLHIKGNNKEGPLGKEAIWHLAERQTGKRDFSREIELPENVKLDQIKAQVENGLLTIVVPKDTAPRSSKVRNINITSKL

Homology

[Close]

BLAST of Lag0029358 vs. NCBI nr
Match: KAG6579046.1 (15.7 kDa heat shock protein, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016570.1 15.7 kDa heat shock protein, peroxisomal [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 241.9 bits (616), Expect = 4.2e-60
Identity = 120/141 (85.11%), Postives = 130/141 (92.20%), Query Frame = 0


Query: 39  MADDLFGYPLRRFFWSPAVFRGPSAAVALLDWLETSNAHIFKVNVPGFSKDDIKVQVDEG 98
           MA+DLFGYPLRRFFWSPAVFRGPS  +AL+DWLETSNAHIFKV+VPGFSKD+I VQVDE 
Sbjct: 1   MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA 60

Query: 99  NVLHIKG-NNKEGPLGKEAIWHLAERQTGKRDFSREIELPENVKLDQIKAQVENGLLTIV 158
           NVLHI+G + KE   GKEAIWHLAERQTGKR FSREIELPENVKLD+IKAQVE+GLLTIV
Sbjct: 61  NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV 120

Query: 159 VPKDTAPRSSKVRNINITSKL 179
           VP+DTAPR SKVRNINITSKL
Sbjct: 121 VPRDTAPRPSKVRNINITSKL 141

[Close]

BLAST of Lag0029358 vs. NCBI nr
Match: XP_022141385.1 (15.7 kDa heat shock protein, peroxisomal [Momordica charantia] >XP_022141386.1 15.7 kDa heat shock protein, peroxisomal [Momordica charantia])

HSP 1 Score: 241.9 bits (616), Expect = 4.2e-60
Identity = 120/141 (85.11%), Postives = 128/141 (90.78%), Query Frame = 0


Query: 39  MADDLFGYPLRRFFWSPAVFRGPSAAVALLDWLETSNAHIFKVNVPGFSKDDIKVQVDEG 98
           MADD FGYPLRRFFWSPAVFR P  AVALLDWLETSNAH FKVNVPGFSKD+IKVQVDEG
Sbjct: 1   MADDFFGYPLRRFFWSPAVFREPFGAVALLDWLETSNAHTFKVNVPGFSKDEIKVQVDEG 60

Query: 99  NVLHIKGN-NKEGPLGKEAIWHLAERQTGKRDFSREIELPENVKLDQIKAQVENGLLTIV 158
           N+LHIKG+  KE P+ K+ IWHLAERQ  KR+FSREIELPENVK+DQIKAQVENG+LTIV
Sbjct: 61  NILHIKGSRGKEEPVAKDTIWHLAERQREKREFSREIELPENVKVDQIKAQVENGVLTIV 120

Query: 159 VPKDTAPRSSKVRNINITSKL 179
           VPKDTA RSSKVRNINITSKL
Sbjct: 121 VPKDTATRSSKVRNINITSKL 141

[Close]

BLAST of Lag0029358 vs. NCBI nr
Match: XP_022939289.1 (15.7 kDa heat shock protein, peroxisomal [Cucurbita moschata])

HSP 1 Score: 241.1 bits (614), Expect = 7.2e-60
Identity = 120/141 (85.11%), Postives = 129/141 (91.49%), Query Frame = 0


Query: 39  MADDLFGYPLRRFFWSPAVFRGPSAAVALLDWLETSNAHIFKVNVPGFSKDDIKVQVDEG 98
           MA+DLFGYPLRRFFWSP VFRGPS  +AL+DWLETSNAHIFKV+VPGFSKD+I VQVDE 
Sbjct: 1   MANDLFGYPLRRFFWSPPVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA 60

Query: 99  NVLHIKG-NNKEGPLGKEAIWHLAERQTGKRDFSREIELPENVKLDQIKAQVENGLLTIV 158
           NVLHI+G + KE   GKEAIWHLAERQTGKR FSREIELPENVKLD+IKAQVE+GLLTIV
Sbjct: 61  NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV 120

Query: 159 VPKDTAPRSSKVRNINITSKL 179
           VPKDTAPR SKVRNINITSKL
Sbjct: 121 VPKDTAPRPSKVRNINITSKL 141

[Close]

BLAST of Lag0029358 vs. NCBI nr
Match: XP_023550919.1 (15.7 kDa heat shock protein, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 240.7 bits (613), Expect = 9.4e-60
Identity = 120/141 (85.11%), Postives = 129/141 (91.49%), Query Frame = 0


Query: 39  MADDLFGYPLRRFFWSPAVFRGPSAAVALLDWLETSNAHIFKVNVPGFSKDDIKVQVDEG 98
           MA+DLFGYPLRRFFWSPAV RGPS  +AL+DWLETSNAHIFKV+VPGFSKD+I VQVDE 
Sbjct: 1   MANDLFGYPLRRFFWSPAVLRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA 60

Query: 99  NVLHIKG-NNKEGPLGKEAIWHLAERQTGKRDFSREIELPENVKLDQIKAQVENGLLTIV 158
           NVLHI+G + KE   GKEAIWHLAERQTGKR FSREIELPENVKLD+IKAQVE+GLLTIV
Sbjct: 61  NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV 120

Query: 159 VPKDTAPRSSKVRNINITSKL 179
           VPKDTAPR SKVRNINITSKL
Sbjct: 121 VPKDTAPRPSKVRNINITSKL 141

[Close]

BLAST of Lag0029358 vs. NCBI nr
Match: XP_022993441.1 (15.7 kDa heat shock protein, peroxisomal [Cucurbita maxima])

HSP 1 Score: 239.2 bits (609), Expect = 2.7e-59
Identity = 118/141 (83.69%), Postives = 130/141 (92.20%), Query Frame = 0


Query: 39  MADDLFGYPLRRFFWSPAVFRGPSAAVALLDWLETSNAHIFKVNVPGFSKDDIKVQVDEG 98
           MA+DLFGYPLRRFFWSPAVFRGPS  +AL+DWLETSNAHIFKV+VPGFSKD+I VQV+E 
Sbjct: 1   MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVNEA 60

Query: 99  NVLHIKG-NNKEGPLGKEAIWHLAERQTGKRDFSREIELPENVKLDQIKAQVENGLLTIV 158
           NVLHI+G + KE   GK+AIWHLAERQTGKR FSRE+ELPENVKLD+IKAQVE+GLLTIV
Sbjct: 61  NVLHIQGTSGKEEAHGKDAIWHLAERQTGKRGFSRELELPENVKLDKIKAQVEDGLLTIV 120

Query: 159 VPKDTAPRSSKVRNINITSKL 179
           VPKDTAPR SKVRNINITSKL
Sbjct: 121 VPKDTAPRPSKVRNINITSKL 141

[Close]

BLAST of Lag0029358 vs. ExPASy Swiss-Prot
Match: Q9FHQ3 (15.7 kDa heat shock protein, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=HSP15.7 PE=2 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 1.6e-38
Identity = 81/144 (56.25%), Postives = 109/144 (75.69%), Query Frame = 0


Query: 39  MAD-DLFGYPLRRFFWSPAVFRGPSAAVALLDWLETSNAHIFKVNVPGFSKDDIKVQVDE 98
           MAD  +F YP RR       F+  S + AL+DW+E++N+HIFK+NVPG++K+DIKVQ++E
Sbjct: 1   MADRGIFLYPFRR-------FQEWSRSTALIDWMESNNSHIFKINVPGYNKEDIKVQIEE 60

Query: 99  GNVLHIKGNNKEGPLGKEAIWHLAERQT---GKRDFSREIELPENVKLDQIKAQVENGLL 158
           GNVL I+G   +    +  +WH+AER+    G  +F R IELPENVK+DQ+KA VENG+L
Sbjct: 61  GNVLSIRGEGIKEEKKENLVWHVAEREAFSGGGSEFLRRIELPENVKVDQVKAYVENGVL 120

Query: 159 TIVVPKDTAPRSSKVRNINITSKL 179
           T+VVPKDT+ +SSKVRN+NITSKL
Sbjct: 121 TVVVPKDTSSKSSKVRNVNITSKL 137

[Close]

BLAST of Lag0029358 vs. ExPASy Swiss-Prot
Match: Q652V8 (16.0 kDa heat shock protein, peroxisomal OS=Oryza sativa subsp. japonica OX=39947 GN=HSP16.0 PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.8e-29
Identity = 67/149 (44.97%), Postives = 98/149 (65.77%), Query Frame = 0


Query: 39  MADDLFGYPLRRFFWS---PAVFRGPSAAVALLDWLETSNAHIFKVNVPGFSKDDIKVQV 98
           MAD  FG P RR  +    P  +   SA  A +DW+ET  +H+ ++NVPG  KDD+KVQV
Sbjct: 1   MADLFFGGPFRRILYGRPFPPDWASASATAA-MDWVETPTSHVLRINVPGLGKDDVKVQV 60

Query: 99  DEGNVLHIKG------NNKEGPLGKEAIWHLAERQTGKRDFSREIELPENVKLDQIKAQV 158
           ++GNVL ++G        KE    K+ +WH+AER  G+ +F+RE+ LP  V+++QI+A V
Sbjct: 61  EDGNVLTVRGAAPHAAAEKEREREKDVVWHVAER--GRPEFAREVALPAEVRVEQIRASV 120

Query: 159 ENGLLTIVVPKDTAPRSSKVRNINITSKL 179
           +NG+LT+VVPK+ AP   + R I ++SKL
Sbjct: 121 DNGVLTVVVPKEPAPARPRTRPIAVSSKL 146

[Close]

BLAST of Lag0029358 vs. ExPASy Swiss-Prot
Match: Q05832 (18.3 kDa class I heat shock protein OS=Oxybasis rubra OX=3560 GN=HSP18 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.3e-23
Identity = 60/142 (42.25%), Postives = 85/142 (59.86%), Query Frame = 0


Query: 35  SLLPMADDLFGYP--LRRFFWSPAVFRGPSAAVALLDWLETSNAHIFKVNVPGFSKDDIK 94
           SL  + D  FG P  L     S       + A A +DW ET  AH+FK ++PG  K+++K
Sbjct: 22  SLDEIWDPFFGLPSTLSTVPRSETAAETAAFANARIDWKETPEAHVFKADLPGVKKEEVK 81

Query: 95  VQVDEGNVLHIKGNNKEGPLGKEAIWHLAERQTGKRDFSREIELPENVKLDQIKAQVENG 154
           V+V++GNVL I G        K   WH  ER +G+  F R+  LPEN K+DQ+KA +ENG
Sbjct: 82  VEVEDGNVLRISGQRAREKEEKNDTWHRVERSSGQ--FMRKFRLPENAKVDQVKAGMENG 141

Query: 155 LLTIVVPKDTAPRSSKVRNINI 175
           +LT+ VPK+ AP+  +V+ IN+
Sbjct: 142 VLTVTVPKNEAPK-PQVKAINV 160

[Close]

BLAST of Lag0029358 vs. ExPASy Swiss-Prot
Match: Q9LNW0 (17.8 kDa class I heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP17.8 PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 5.6e-23
Identity = 52/124 (41.94%), Postives = 87/124 (70.16%), Query Frame = 0


Query: 55  PAVFRGPSAAV--ALLDWLETSNAHIFKVNVPGFSKDDIKVQVDEGNVLHIKGNNKEGPL 114
           P+   G ++A+  A +DW ET+ AH+FK ++PG  K+++KV++++ +VL I G       
Sbjct: 35  PSSLSGETSAITNARVDWKETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERHVEKE 94

Query: 115 GKEAIWHLAERQTGKRDFSREIELPENVKLDQIKAQVENGLLTIVVPK-DTAPRSSKVRN 174
            K+  WH  ER +G+  FSR+ +LPENVK+DQ+KA +ENG+LT+ VPK + A + ++V++
Sbjct: 95  EKQDTWHRVERSSGQ--FSRKFKLPENVKMDQVKASMENGVLTVTVPKVEEAKKKAQVKS 154

Query: 175 INIT 176
           I+I+
Sbjct: 155 IDIS 156

[Close]

BLAST of Lag0029358 vs. ExPASy Swiss-Prot
Match: Q84Q72 (18.1 kDa class I heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP18.1 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 7.3e-23
Identity = 57/126 (45.24%), Postives = 79/126 (62.70%), Query Frame = 0


Query: 55  PAVFRGPSA-----AVALLDWLETSNAHIFKVNVPGFSKDDIKVQVDEGNVLHIKGNNKE 114
           P+  RG S+     A A +DW ET  AH+FK +VPG  K+++KV+V++GNVL I G   +
Sbjct: 38  PSFPRGTSSETAAFAGARIDWKETPEAHVFKADVPGLKKEEVKVEVEDGNVLQISGERSK 97

Query: 115 GPLGKEAIWHLAERQTGKRDFSREIELPENVKLDQIKAQVENGLLTIVVPKDTAPRSSKV 174
               K   WH  ER +GK  F R   LPEN K +QIKA +ENG+LT+ VPK+  P+   V
Sbjct: 98  EQEEKTDKWHRVERSSGK--FLRRFRLPENTKPEQIKASMENGVLTVTVPKE-EPKKPDV 157

Query: 175 RNINIT 176
           ++I +T
Sbjct: 158 KSIQVT 160

The following BLAST results are available for this feature:

BLAST of Lag0029358 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6579046.1	4.2e-60	85.11	15.7 kDa heat shock protein, peroxisomal, partial [Cucurbita argyrosperma subsp....	[more]
XP_022141385.1	4.2e-60	85.11	15.7 kDa heat shock protein, peroxisomal [Momordica charantia] >XP_022141386.1 1...	[more]
XP_022939289.1	7.2e-60	85.11	15.7 kDa heat shock protein, peroxisomal [Cucurbita moschata]	[more]
XP_023550919.1	9.4e-60	85.11	15.7 kDa heat shock protein, peroxisomal [Cucurbita pepo subsp. pepo]	[more]
XP_022993441.1	2.7e-59	83.69	15.7 kDa heat shock protein, peroxisomal [Cucurbita maxima]	[more]

BLAST of Lag0029358 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FHQ3	1.6e-38	56.25	15.7 kDa heat shock protein, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=HSP1...	[more]
Q652V8	1.8e-29	44.97	16.0 kDa heat shock protein, peroxisomal OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q05832	3.3e-23	42.25	18.3 kDa class I heat shock protein OS=Oxybasis rubra OX=3560 GN=HSP18 PE=2 SV=1	[more]
Q9LNW0	5.6e-23	41.94	17.8 kDa class I heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP17.8 P...	[more]
Q84Q72	7.3e-23	45.24	18.1 kDa class I heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 38..175 e-value: 3.8E-32 score: 113.0
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 41..168
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 69..174 e-value: 5.2E-23 score: 81.1
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 59..175 score: 25.183424
None	No IPR available	PANTHER	PTHR11527:SF142	15.7 KDA HEAT SHOCK PROTEIN, PEROXISOMAL	coord: 43..177
None	No IPR available	CDD	cd06472	ACD_ScHsp26_like	coord: 68..160 e-value: 2.44969E-40 score: 129.732
IPR031107	Small heat shock protein HSP20	PANTHER	PTHR11527	HEAT-SHOCK PROTEIN 20 FAMILY MEMBER	coord: 43..177

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0029358.1	Lag0029358.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051259	protein complex oligomerization
biological_process	GO:0006457	protein folding
biological_process	GO:0009408	response to heat
biological_process	GO:0042542	response to hydrogen peroxide
biological_process	GO:0009651	response to salt stress
molecular_function	GO:0043621	protein self-association
molecular_function	GO:0051082	unfolded protein binding