Lag0029325 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0029325
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr8: 37738072 .. 37738844 (-)
RNA-Seq ExpressionLag0029325
SyntenyLag0029325
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATTGTTGTTGGCGAAGTTCAACCGTAGCTTCTCGAATAGATGGTTTGAGCTTCATCACGCTCTTTATTCTGAGGAGATCTAGCTGATTGCTATGATCTGTTGGACAATTTGGACAGATAGAAACAATGTTGTACATGAGAAAGTTGTTCCTCCTCTAGATATTCACAACAGATGGATTAAAAATTATCTAAGGGAGTACTTATAGACAAATTCTAATAATAATCGATCCCCTGTTGATAATTCAGTAGTTCAAGAGCAAATGTCAGCCTGAAATCCGCCTCCGACGAACTGGGTAAAGATTAATGTTGACGTTGCTTGGAAGGCAGGTTTGCCTCGCACAGGAATTGGCGTTATATGTCGCGATTCTTCCGGATTATTGCTAGGAGCTTCATCTGCTTTTGTCAGCCTGGATTTTGACCCTCCTTTAGCCGAATTGCGGACTCTTTCAAAAGGCATGAAGTTGGCGATCTCCCTAGGTTACGGAAATGTGATTTTGGATTCTGATTGTCTCCATGCGATTAATCTCATTAAGGGAGAATTGGAGGTGCGGAATGAACTGGGAATTATTATCGATGATCTTCGAGCAAAGACTTCCCTATTCAATGAGCTTATTTTTTCTTTTTCACCTAAAAGAGTCAACTATGCAGCTGACTCCATAGCTAAGAGAGCTTTAAGAGAGGAATGTAATGAAATTTGGATCCACGAGTTTCCTGAGTGGATTGTTAATCTGGTCATTCATGACCGTTTGTCTGTTGCCCTTGAGGCATAA

mRNA sequence

ATGAATTGTTGTTGGCGAAGTTCAACCGTAGCTTCTCGAATAGATGGTTTGCCTCGCACAGGAATTGGCGTTATATGTCGCGATTCTTCCGGATTATTGCTAGGAGCTTCATCTGCTTTTGTCAGCCTGGATTTTGACCCTCCTTTAGCCGAATTGCGGACTCTTTCAAAAGGCATGAAGTTGGCGATCTCCCTAGGTTACGGAAATGTGATTTTGGATTCTGATTGTCTCCATGCGATTAATCTCATTAAGGGAGAATTGGAGGTGCGGAATGAACTGGGAATTATTATCGATGATCTTCGAGCAAAGACTTCCCTATTCAATGAGCTTATTTTTTCTTTTTCACCTAAAAGAGTCAACTATGCAGCTGACTCCATAGCTAAGAGAGCTTTAAGAGAGGAATGTAATGAAATTTGGATCCACGAGTTTCCTGAGTGGATTGTTAATCTGGTCATTCATGACCGTTTGTCTGTTGCCCTTGAGGCATAA

Coding sequence (CDS)

ATGAATTGTTGTTGGCGAAGTTCAACCGTAGCTTCTCGAATAGATGGTTTGCCTCGCACAGGAATTGGCGTTATATGTCGCGATTCTTCCGGATTATTGCTAGGAGCTTCATCTGCTTTTGTCAGCCTGGATTTTGACCCTCCTTTAGCCGAATTGCGGACTCTTTCAAAAGGCATGAAGTTGGCGATCTCCCTAGGTTACGGAAATGTGATTTTGGATTCTGATTGTCTCCATGCGATTAATCTCATTAAGGGAGAATTGGAGGTGCGGAATGAACTGGGAATTATTATCGATGATCTTCGAGCAAAGACTTCCCTATTCAATGAGCTTATTTTTTCTTTTTCACCTAAAAGAGTCAACTATGCAGCTGACTCCATAGCTAAGAGAGCTTTAAGAGAGGAATGTAATGAAATTTGGATCCACGAGTTTCCTGAGTGGATTGTTAATCTGGTCATTCATGACCGTTTGTCTGTTGCCCTTGAGGCATAA

Protein sequence

MNCCWRSSTVASRIDGLPRTGIGVICRDSSGLLLGASSAFVSLDFDPPLAELRTLSKGMKLAISLGYGNVILDSDCLHAINLIKGELEVRNELGIIIDDLRAKTSLFNELIFSFSPKRVNYAADSIAKRALREECNEIWIHEFPEWIVNLVIHDRLSVALEA
Homology
BLAST of Lag0029325 vs. NCBI nr
Match: XP_023893303.1 (uncharacterized protein LOC112005292 [Quercus suber])

HSP 1 Score: 85.5 bits (210), Expect = 4.6e-13
Identity = 42/136 (30.88%), Postives = 77/136 (56.62%), Query Frame = 0

Query: 19  RTGIGVICRDSSGLLLGASSAFVSLDFDPPLAELRTLSKGMKLAISLGYGNVILDSDCLH 78
           R GIGV+ RDS G++LG+ S  +S  + P   E   ++  M+ A+ LG+ +VIL++D L 
Sbjct: 64  RAGIGVVIRDSEGMVLGSLSKQISHAYSPLEIEAMAVATAMQFALDLGFQHVILETDSLV 123

Query: 79  AINLIKGELEVRNELGIIIDDLRAKTSLFNELIFSFSPKRVNYAADSIAKRALREECNEI 138
            +  +  + E  + +G+++D++R   +LFNEL +S   +  N  A  +A  A+      +
Sbjct: 124 LVKALSDDTEFFSTVGLVLDEIRIMVNLFNELHYSHVKREGNIVAHKLAHHAICVSDVVV 183

Query: 139 WIHEFPEWIVNLVIHD 155
           W+ + P  +  +V+ D
Sbjct: 184 WMEDVPPLLFLVVLAD 199

BLAST of Lag0029325 vs. NCBI nr
Match: XP_038704726.1 (uncharacterized protein LOC120000672 [Tripterygium wilfordii])

HSP 1 Score: 84.7 bits (208), Expect = 7.9e-13
Identity = 43/138 (31.16%), Postives = 75/138 (54.35%), Query Frame = 0

Query: 21  GIGVICRDSSGLLLGASSAFVSLDFDPPLAELRTLSKGMKLAISLGYGNVILDSDCLHAI 80
           G+G + RD +G +  A S  ++  F P + +   L KG +LAI +G  N+I++SD    +
Sbjct: 137 GVGAVIRDHNGQVCAALSECMAGAFGPQVVKAYALMKGTQLAIHMGVSNLIIESDSHVLV 196

Query: 81  NLIKGELEVRNELGIIIDDLRAKTSLFNELIFSFSPKRVNYAADSIAKRALREECNEIWI 140
           N I+ E       G I+DD+R+  S    +   ++ +  N  A  +A+ ++  +C   WI
Sbjct: 197 NAIRSEAYELAPFGHILDDIRSLISCIGSVSLRYTRRLCNRVAHKLARNSINFDCPMRWI 256

Query: 141 HEFPEWIVNLVIHDRLSV 159
            E P++I++ VIHD + V
Sbjct: 257 GECPDFILSAVIHDSIPV 274

BLAST of Lag0029325 vs. NCBI nr
Match: KMS97072.1 (hypothetical protein BVRB_7g179330 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 84.0 bits (206), Expect = 1.3e-12
Identity = 44/136 (32.35%), Postives = 73/136 (53.68%), Query Frame = 0

Query: 19  RTGIGVICRDSSGLLLGASSAFVSLDFDPPLAELRTLSKGMKLAISLGYGNVILDSDCLH 78
           R G+G++ RD +G +L A S  +S D+    AE   +    + AI+ G+ NV+L+SD   
Sbjct: 674 RVGLGMVARDENGQVLRAWSRTMSHDWPAEKAEAAAVLWATEEAIAQGWSNVVLESDAQV 733

Query: 79  AINLIKGELEVRNELGIIIDDLRAKTSLFNELIFSFSPKRVNYAADSIAKRALREECNEI 138
            IN I   L+ R ++  +++D+      F  + FSF  +  N  A  +AK A    C+E+
Sbjct: 734 IINAINSRLQRRGDIQAMLEDVLNLVPSFETIKFSFCYRECNEIAHRLAKWATSNFCDEV 793

Query: 139 WIHEFPEWIVNLVIHD 155
           W+   P WI +L++ D
Sbjct: 794 WLVGGPSWIADLIVSD 809

BLAST of Lag0029325 vs. NCBI nr
Match: XP_023919081.1 (uncharacterized protein LOC112030645 [Quercus suber])

HSP 1 Score: 82.8 bits (203), Expect = 3.0e-12
Identity = 39/134 (29.10%), Postives = 75/134 (55.97%), Query Frame = 0

Query: 21  GIGVICRDSSGLLLGASSAFVSLDFDPPLAELRTLSKGMKLAISLGYGNVILDSDCLHAI 80
           G+GV+ R+S GL+LG+ S  V L + P   E   ++  ++ A  LG+   I+++D L  +
Sbjct: 700 GVGVVIRNSEGLVLGSLSKLVPLAYSPLEIEATAIATALEFASDLGFQRAIIETDSLVLV 759

Query: 81  NLIKGELEVRNELGIIIDDLRAKTSLFNELIFSFSPKRVNYAADSIAKRALREECNEIWI 140
             ++ + E  + +G+I+D++R K + FNEL +S   +  N  A  +A+ A+      +W+
Sbjct: 760 KALREDSEFLSAVGLILDEIRRKVNFFNELHYSHVKREGNIVAHKLARHAISVSDIVVWM 819

Query: 141 HEFPEWIVNLVIHD 155
            + P  +  +V+ D
Sbjct: 820 EDVPPLLFPVVLAD 833

BLAST of Lag0029325 vs. NCBI nr
Match: XP_021859888.1 (uncharacterized protein LOC110798996 [Spinacia oleracea])

HSP 1 Score: 82.4 bits (202), Expect = 3.9e-12
Identity = 41/135 (30.37%), Postives = 75/135 (55.56%), Query Frame = 0

Query: 21  GIGVICRDSSGLLLGASSAFVSLDFDPPLAELRTLSKGMKLAISLGYGNVILDSDCLHAI 80
           G+G++ RD  G +L ++ +    + DP  AE + +  G K A  +G+ +++++SDC + +
Sbjct: 82  GLGMVVRDEVGDVLMSAGSSCQAEGDPLHAEAKAMFFGFKYAFDVGFRSIVMESDCANLV 141

Query: 81  NLIKGELEVRNELGIIIDDLRAKTSLFNELIFSFSPKRVNYAADSIAKRALREECNEIWI 140
            L  G+  VR    +I+DD+ A    F   +F F+ +  N  A SIAK +L  E   +W+
Sbjct: 142 QLAMGKTRVRTRTQVIVDDILALAPNFEYCLFVFAIRSCNRVAHSIAKSSLTLEEVVVWM 201

Query: 141 HEFPEWIVNLVIHDR 156
            + P  ++ LV+ D+
Sbjct: 202 EDHPLDVLPLVLADK 216

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023893303.14.6e-1330.88uncharacterized protein LOC112005292 [Quercus suber][more]
XP_038704726.17.9e-1331.16uncharacterized protein LOC120000672 [Tripterygium wilfordii][more]
KMS97072.11.3e-1232.35hypothetical protein BVRB_7g179330 [Beta vulgaris subsp. vulgaris][more]
XP_023919081.13.0e-1229.10uncharacterized protein LOC112030645 [Quercus suber][more]
XP_021859888.13.9e-1230.37uncharacterized protein LOC110798996 [Spinacia oleracea][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 19..130
e-value: 1.1E-16
score: 60.8
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 9..133
e-value: 4.4E-10
score: 41.7
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 18..154
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 18..154
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 9..128
e-value: 6.95309E-16
score: 67.7244
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 19..140

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0029325.1Lag0029325.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity