Lag0029272 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0029272
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	biotin--protein ligase 2-like
Location	chr8: 37096230 .. 37101644 (+)
RNA-Seq Expression	Lag0029272
Synteny	Lag0029272

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTTTAACTAACCCACTTCGCTATTTCAGCAGGCGAGACTCGCGCTACATTTTTTCCATCATAAATTCTCCCTCTCTGAGGTCTCTCAGCAAAACAACTGCTTCAGCTCCCACCCCAGGTTTGTTTAATGCTTGTTTCAATAAATGCTGGAGTGATTTTCCTGAAAATCTGGGTTCATTACGTTGATTTGGGACGAATTTTAATTTGCGGAAGTTGGGTCGAACTGTTTCATAAAGATTTATGCGTTTTTCTGAAAAGAAGCTGAATAACCATATGGGGTATTGGTATTCTTCTGGAAAACAGAAACTTCCATTAATGTGAGGGAAAAAAGGAGATTGCTCTCTATATTATCTGGTTCAGCAATGGAGACTAGTTCAACATGTGCGTTGGTTTTGAGTGGAAAAACAGCGGCTGAGAATGAAACTGCCAAGTTGCTGAAGAGAAATGACACTTTGAAGCTCCATGACGGTACTGGAATTTCGGTTTCTTTGCACTCAGAAAGGGATAAATCTATGGAGGAAAGTGGTTTTCAGATTGATTTCTATATGGATGCTCTTTCTACTAATACTTATGGTAGATTCCTCATTTGGTGTCCACGGATTCCTTCAACTCATGATGTCATTTCTCAGTAAGTGTATCAAAGCATTTTTTTTCCTCACCATTTTTTCATTCTTTGACGAGCTAGCTAACTATTAGTTTGCAACAGCAACTTTAGCGACCTTCCATTGGGTGCTGTCTGTGTGGCTGATGTACAGTTTAAGGGAAGAGGTGTGTAATACTAATAAGTAAAGACAAACTGGTAACATTGTTTGTTAGTTCTGGAATTCCTCTTTCTATTTAAAGTCACTTTGTGGATTTAAGTGAAGGTCGATCAAAGAATTTGTGGGAATCACCCCCTGGTTGCCTCATGTTTTCCTTTACCATTCAAATGGAAGATGGGCGTATTGTTCCTCTGCTGCAGTATGTAATATCTCTCGCTATTACCGAGGCCATAAAAGATATTTGCGACAAAAAGGTAACTTTTGTCATGTTTTGGCCCATATTAGCAACCCTTATGGAATCTTTGTTGGAATTTGGTAACTTGATTCTGGATCCCGTGATATGCTAAATAACCAAAACTTTACGGGTAGGGGATTAAATGAAATTGCAAGAGAAAAACTTATGGTCATGGAAGGAGGAAACAGTCAGAAAGAATCGGGGGGCTTGAGGAGTATATAGGATTTCAAGTAATGGTAGACTGGCTTCCTATATCCAGAACTGTAAACGTGCACTATGGAGATGTTTATACTCCCTTACATATTTCGTTATTTCTACCGTGTCAATGACACATAGCTATCACACTCATGTTGTGACTATTATTTGTAAGCTTTTGAGGTTAATACCGCTCATTCTCTTTGTGTTTCTATTTCAGGGTTTACCTTATATTGATTTGAAGATAAAGTGGCCAAACGATCTTTATGTGAATGGCCTGAAAGTTGGAGGCATTCTATGCACTTCAACATATAGATCAAAGAAGTTCAACGTTAGTGCTGGTAATTGTGTTTGTTTCCTTTTCCATCTATTTTTCTTCTACAGTCCTTTTTCCCCTTCCAAATTTAAACTTATAGTCTTATTGCACCAATTTGAAAAGCCTAGAGTCACTGTGATATATTTTAACTGCTGTGTTCTTCCAGGTATAGGTTTGAATGTAGACAACGACAAACCAACAACATGCTTGAACGAAGCTCTTACAAATTTATCAAGTACACCTTACGAGCTCAGAAGAGAGGATATCTTAGCATTCTTCTTTAACAAATTTGAAAGACTGTACGATATTTTTATAAATCAAGGTATTTATATGTCAATCTAACGTCTTCCTCATGATAAGACATTGTCACATTCATGCATGGATTTAGAGCTTCTCCTTCAATCCAACCTTAACAATGCTCTGGAGAAGCTTGTGAGTTATGAAAATTCTAAGCAGCTATCATCATTTGCGAAAAGAATTTGAAATTGTCCATCTCTCTTCTTTTTCCTCATCACTGAGCATGTTAGTGTGAACATCATTCTATCAATTCATGCTCTTCGTTGCCTTAAGGTTTTGTGCATGCTAAGCATTTGGCTTCTACCCATGGCATACAGGATAGGACTTCAGATGAATAATTATACGTAAAGGAAATCTTCCTAGGTTTTCTAGTTACACATCCATCAAGTCTGTAAAGATGATAATTGATAGCTATATGTAATACTTCCTCTGAAGGGGGTGAGAGAGAGAGCTAAGATATTATTCGTTTAGGATGTAGATTTTTAATCTTGTTCTCTTGTGAAAAAGCTAGAATTTTTGTATCACACGTATTTCATTTATTGTTACCTTCCTAACATGCTGTCATACCTTCAAATATAGTCCACATCATGTTTATCTCAAGTTTGTTATGCTTCAATTTCGGAATTTCCCAGGTGACCTCATTCTGACTAAATCCGTGAATAATGGCATTTTGTTTTCATCATTTCTGAATAATTAGGGTTTCAGGCTCTTCAGGAACTCTACTGTCGGACATGGTTGCACAGGTAACTAATGATGTGGATTAATTTATCTTCTTTATATATATATATATTCAACACATATTGTGTTCCAAGATTCTCACGATTATACTTGGTTTGCATTGTCTATACATTTGTTGTCATGGAAACCAAAAACATCGTGGAAATGGAAAATGTTGTTTGATTCTTTAAGTGCTTAATGTCTATGGAGCTAGTAATACTTTGCTTATGTTGCCGTGGGACTCTGTCTTTGCACAAGTCAAACGTCACTTGTTTCAATTGTTTTGTTGACAATTTATGACATATAATATTGTGCAAGTTATGCTAAATAGGTCTTACACTTTCTTCCCTATAGATATGACTTTGTTTGGATCTGTCTTGTAGTGGGCAAAGAGTTATTGTACAAGAAAAGAAAGGAGACCAAGTAGTGGAAAATGTAGTCACTATTCAGGTACGTGATATCTGAATGATAGCTGATCCTGTGGCAGCTCTAGTATTTACACGAATGCTGCAAAGTGCAAACTTAATCCCACTACTCTTTTTGGTTCTTCACTGTGGTTATTTATATATTTTTGGCTCAGAAGAAAAGACCTTGTCTCCTGCAGTTGAATTTGTTTTTATCTTGTAGTTGTTAGATAATCCTATTCCTGAAGGATGTTAAATATTTGGCATCAATTTTTTGTTCAACTTAGTACTGGAAAATGCTGAATGCACTACATTCTATGATTTTCATGGTTTTAAACACTAAATTCGTTGCTTATTGGTCAGAAATTCTTGTTTTCTTATATAGGGGTTGACATCTTCAGGATATTTGCTAGCTATTGGAGATGACCACCAAATGTGCGAGCTTCATCCCGATGGAAATAGGTCAAGTATTTGAATGGATTCCTATTCTTGATTTAGTGAATTAACTATGCTGTGCTGATTTTTTTGCAAAGTTTGACACATACAATTTGATACATCGACAGCGAGTGTACGACATCTGTGTCTATAGCATATCAATTGCATTTTGAAACTAATGCTGAAAGATTGAGAGAGTTCATTGTACATAAAGAAAAGTGACTTTGATATATGTATACCATTTGCCCCAACCTGAACGTTCAAACAACAAATAGGATAACTTGATAAGTCATCTGCTTCAGGCTTTCACCGAAAATCTGGTCACAACATTCTAAAATTTTTTATTTCCTGTTTATACTGTAGATAGATGCTTACGAAAAGGAAAAGGTCTATCATAACTAAACTAAATCATTATTATTTACAAAATTGACTGAAATTGATTCCAAATTCATGCATGACTAAGAAACAGTGGTCAATAAAGTTAACGTCTTCAAAGTGCTTTAGAATCAATCATAATGATTATAAAGGAAGTGACTGCCCTGTTGGCCAGTCAACCAATATTGTAGTTTCTGCTGTACTCAGTTGGATGAATTTTTAATTCTGATTTATTTATTGCTCAAAAGTCCTTTAGAACTAAGCTGGCAGAAATGTCTACATGCCCTGTTCTGCATTGCATGCATCAGCTCACAACCCCAAGCATTTGAAAAATTTAACCATCTGAATTCTTCTCCAAATGCAATTGACTGTAGAGATCTGATTTTAGAAGGCTAATAATATGTAATAGATCTGATCTTAAATCTGATGATCATGTCATCTTCCTATTGTTCTACAGTTTGGACTTTTTCAAAGGACTGATCAAGAGAAAACTGGAGTGAGAAGGCTGCTTTTTCACTTTCCAACAGGTGGCTGACTATGAGCTGTATGGAATGGAATCATGTGCAATAAGAAGGAGACATTTGCTCTCGTTTATTTTTGGCTAACAATTCTCTGTTTCTAAACTGGAGGAAAGAAAGAGAAAGATATCTTACTTTTTATGATTAAACAATGAGATCTCTACTGAAGTTGTAAATATTATTTATCTTTAAACTTACTAAGCAGGGTCCATGGCCCAGAACGTGCTATATTTTTGTATCATCCTATTGAACGTGAAGGAATTCCGAGTGGGACAATTTCCAATTGATTAAAACTGTCCCCACAATTGGCATAATCAGTCAGTCAGCAGTTCTTTTTTGTTAGAAACTTAGGAAATTCTCTGCCACTTTATTGCCAGCTGTTTGAGCTTGAAACTGAAACCCCGTTATGACAGAAGGTCTTGCGTTGTAATGGAGAACGGATGGAACTTATTTGAACTTTACAACTGTGAAATACGTGAAAAGAAACAGAAGGGCAAAAGATTCTTGTGGCGAGGCGCCATGTCAGACCCAGAAACTGGTATGTCTTCTTCTATACATTTCCTTACGGTTGTTGGGGCCTTTTGTTTAGGGTTCACTATCAGAATAGAGAAAAGGGATTGGCTTGCCACATGCAGAACAGTGGATGGATTGAAAAACCAAAAAAGGGTTCTATCATCATAACATAAGTTTGTTCTCTTAACTTTCAATATCGTTTTTGTACCTAATAGTTTTCGAGACATTACATGCTAACTATACTATTAAAGTTAGAGTTAGGTGGTGGGCTGATTTCAGGCTGGCTGAGGTGTTTGGTAGTGTAAGCATTGAAATGACGTAGTTAACGTCATGAACTTATTAGACACAGACTTGATGGAAACTCGTGAAACTTAGGGACAACCGTCCTAGTTCAAGTCAGCTTTGTATTGGTTTTGGAGGGGGTGGGGGTCATGCAAGGACAGAGTTGGGCGGTGGGAGTGTGGTGTTTGAGGTAAACAAAAGCTGCAAAAGTGAGAAGCCAGAAACTGTGAAACTGGGTTTGTGTTCAGCTTATGTTACGATGTGCCCCCCAAGTAGAATTCCCAATTCTGAGTTGCCGCTGTTGCATCCTTTTGTCTGTCTTCGGCTTTGTCTTATAATCAGCTCAACCTCAATGATGCGGTAG

mRNA sequence

ATGATTTTAACTAACCCACTTCGCTATTTCAGCAGGCGAGACTCGCGCTACATTTTTTCCATCATAAATTCTCCCTCTCTGAGGTCTCTCAGCAAAACAACTGCTTCAGCTCCCACCCCAGAAACTTCCATTAATGTGAGGGAAAAAAGGAGATTGCTCTCTATATTATCTGGTTCAGCAATGGAGACTAGTTCAACATGTGCGTTGGTTTTGAGTGGAAAAACAGCGGCTGAGAATGAAACTGCCAAGTTGCTGAAGAGAAATGACACTTTGAAGCTCCATGACGGTACTGGAATTTCGGTTTCTTTGCACTCAGAAAGGGATAAATCTATGGAGGAAAGTGGTTTTCAGATTGATTTCTATATGGATGCTCTTTCTACTAATACTTATGGTAGATTCCTCATTTGGTGTCCACGGATTCCTTCAACTCATGATGTCATTTCTCACAACTTTAGCGACCTTCCATTGGGTGCTGTCTGTGTGGCTGATGTACAGTTTAAGGGAAGAGGTCGATCAAAGAATTTGTGGGAATCACCCCCTGGTTGCCTCATGTTTTCCTTTACCATTCAAATGGAAGATGGGCGTATTGTTCCTCTGCTGCAGTATGTAATATCTCTCGCTATTACCGAGGCCATAAAAGATATTTGCGACAAAAAGGGTTTACCTTATATTGATTTGAAGATAAAGTGGCCAAACGATCTTTATGTGAATGGCCTGAAAGTTGGAGGCATTCTATGCACTTCAACATATAGATCAAAGAAGTTCAACGTTAGTGCTGGTATAGGTTTGAATGTAGACAACGACAAACCAACAACATGCTTGAACGAAGCTCTTACAAATTTATCAAGTACACCTTACGAGCTCAGAAGAGAGGATATCTTAGCATTCTTCTTTAACAAATTTGAAAGACTGTACGATATTTTTATAAATCAAGGGTTTCAGGCTCTTCAGGAACTCTACTGTCGGACATGGTTGCACAGTGGGCAAAGAGTTATTGTACAAGAAAAGAAAGGAGACCAAGTAGTGGAAAATGTAGTCACTATTCAGGGGTTGACATCTTCAGGATATTTGCTAGCTATTGGAGATGACCACCAAATGTGCGAGCTTCATCCCGATGGAAATAGCTGTTTGAGCTTGAAACTGAAACCCCGTTATGACAGAAGGTCTTGCGTTGTAATGGAGAACGGATGGAACTTATTTGAACTTTACAACTGTGAAATACGTGAAAAGAAACAGAAGGGCAAAAGATTCTTGTGGCGAGGCGCCATGTCAGACCCAGAAACTGTTTTCGAGACATTACATGCTAACTATACTATTAAAGTTAGAGTTAGGTGGTGGGCTGATTTCAGGCTGGCTGAGGTGTTTGGTAGTCTTTGTATTGGTTTTGGAGGGGGTGGGGGTCATGCAAGGACAGAGTTGGGCGGTGGGAGTGTGGTGTTTGAGGTAAACAAAAGCTGCAAAAGTGAGAAGCCAGAAACTGTGAAACTGGGTTTGTGTTCAGCTTATGTTACGATGTGCCCCCCAAGTAGAATTCCCAATTCTGAGTTGCCGCTGTTGCATCCTTTTGTCTGTCTTCGGCTTTGTCTTATAATCAGCTCAACCTCAATGATGCGGTAG

Coding sequence (CDS)

ATGATTTTAACTAACCCACTTCGCTATTTCAGCAGGCGAGACTCGCGCTACATTTTTTCCATCATAAATTCTCCCTCTCTGAGGTCTCTCAGCAAAACAACTGCTTCAGCTCCCACCCCAGAAACTTCCATTAATGTGAGGGAAAAAAGGAGATTGCTCTCTATATTATCTGGTTCAGCAATGGAGACTAGTTCAACATGTGCGTTGGTTTTGAGTGGAAAAACAGCGGCTGAGAATGAAACTGCCAAGTTGCTGAAGAGAAATGACACTTTGAAGCTCCATGACGGTACTGGAATTTCGGTTTCTTTGCACTCAGAAAGGGATAAATCTATGGAGGAAAGTGGTTTTCAGATTGATTTCTATATGGATGCTCTTTCTACTAATACTTATGGTAGATTCCTCATTTGGTGTCCACGGATTCCTTCAACTCATGATGTCATTTCTCACAACTTTAGCGACCTTCCATTGGGTGCTGTCTGTGTGGCTGATGTACAGTTTAAGGGAAGAGGTCGATCAAAGAATTTGTGGGAATCACCCCCTGGTTGCCTCATGTTTTCCTTTACCATTCAAATGGAAGATGGGCGTATTGTTCCTCTGCTGCAGTATGTAATATCTCTCGCTATTACCGAGGCCATAAAAGATATTTGCGACAAAAAGGGTTTACCTTATATTGATTTGAAGATAAAGTGGCCAAACGATCTTTATGTGAATGGCCTGAAAGTTGGAGGCATTCTATGCACTTCAACATATAGATCAAAGAAGTTCAACGTTAGTGCTGGTATAGGTTTGAATGTAGACAACGACAAACCAACAACATGCTTGAACGAAGCTCTTACAAATTTATCAAGTACACCTTACGAGCTCAGAAGAGAGGATATCTTAGCATTCTTCTTTAACAAATTTGAAAGACTGTACGATATTTTTATAAATCAAGGGTTTCAGGCTCTTCAGGAACTCTACTGTCGGACATGGTTGCACAGTGGGCAAAGAGTTATTGTACAAGAAAAGAAAGGAGACCAAGTAGTGGAAAATGTAGTCACTATTCAGGGGTTGACATCTTCAGGATATTTGCTAGCTATTGGAGATGACCACCAAATGTGCGAGCTTCATCCCGATGGAAATAGCTGTTTGAGCTTGAAACTGAAACCCCGTTATGACAGAAGGTCTTGCGTTGTAATGGAGAACGGATGGAACTTATTTGAACTTTACAACTGTGAAATACGTGAAAAGAAACAGAAGGGCAAAAGATTCTTGTGGCGAGGCGCCATGTCAGACCCAGAAACTGTTTTCGAGACATTACATGCTAACTATACTATTAAAGTTAGAGTTAGGTGGTGGGCTGATTTCAGGCTGGCTGAGGTGTTTGGTAGTCTTTGTATTGGTTTTGGAGGGGGTGGGGGTCATGCAAGGACAGAGTTGGGCGGTGGGAGTGTGGTGTTTGAGGTAAACAAAAGCTGCAAAAGTGAGAAGCCAGAAACTGTGAAACTGGGTTTGTGTTCAGCTTATGTTACGATGTGCCCCCCAAGTAGAATTCCCAATTCTGAGTTGCCGCTGTTGCATCCTTTTGTCTGTCTTCGGCTTTGTCTTATAATCAGCTCAACCTCAATGATGCGGTAG

Protein sequence

MILTNPLRYFSRRDSRYIFSIINSPSLRSLSKTTASAPTPETSINVREKRRLLSILSGSAMETSSTCALVLSGKTAAENETAKLLKRNDTLKLHDGTGISVSLHSERDKSMEESGFQIDFYMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLPLGAVCVADVQFKGRGRSKNLWESPPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLSSTPYELRREDILAFFFNKFERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAIGDDHQMCELHPDGNSCLSLKLKPRYDRRSCVVMENGWNLFELYNCEIREKKQKGKRFLWRGAMSDPETVFETLHANYTIKVRVRWWADFRLAEVFGSLCIGFGGGGGHARTELGGGSVVFEVNKSCKSEKPETVKLGLCSAYVTMCPPSRIPNSELPLLHPFVCLRLCLIISSTSMMR

Homology

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BLAST of Lag0029272 vs. NCBI nr
Match: XP_023517017.1 (biotin--protein ligase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 651.7 bits (1680), Expect = 5.3e-183
Identity = 325/375 (86.67%), Postives = 342/375 (91.20%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIINSPSLRSLSKTTASAPTPETSINVREKRRLLSILSGSA 60
           M LTNPLR FS R SR   SIINSPS RS   TTASAPTP TS  V EK RL S+LSG A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIINSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLLSGLA 60

Query: 61  METSSTCALVLSGKTAAENETAKLLKRNDTLKLHDGTGISVSLHSERDKSMEESGFQIDF 120
           METSSTC+LVLSGKT AENETAKLLKRNDTLKL D   IS+SLHSERDKS++ESGFQI  
Sbjct: 61  METSSTCSLVLSGKTEAENETAKLLKRNDTLKLPDDIEISISLHSERDKSLKESGFQIGS 120

Query: 121 YMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180
           YM++LST+T+GRFLIWC RIPST DVIS NFSDLPLGAVCVADVQFKGRGRSKNLWESPP
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGRIVPLLQYV+SLAITEA+KDICDKKGLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVVSLAITEAVKDICDKKGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLSSTPYELRREDILAFFFNK 300
           VGGILCTSTYRSKKFNV+AGIGLNVDNDKPTTCLNEAL NLSSTPY+ RREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

Query: 301 FERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FE LYDIFINQGFQAL+ELYC+TWLHSGQRVIVQEKK DQVVENVVTIQGLT +GYLLAI
Sbjct: 301 FEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVENVVTIQGLTPTGYLLAI 360

Query: 361 GDDHQMCELHPDGNS 376
           GDD+QMCELHPDGNS
Sbjct: 361 GDDNQMCELHPDGNS 375

[Close]

BLAST of Lag0029272 vs. NCBI nr
Match: XP_022957207.1 (biotin--protein ligase 2-like [Cucurbita moschata])

HSP 1 Score: 651.0 bits (1678), Expect = 9.1e-183
Identity = 326/375 (86.93%), Postives = 342/375 (91.20%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIINSPSLRSLSKTTASAPTPETSINVREKRRLLSILSGSA 60
           M LTNPLR FS R SR   SIINSPS RS   TTASAPTP TS  V EK RL S+LSG A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIINSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLLSGLA 60

Query: 61  METSSTCALVLSGKTAAENETAKLLKRNDTLKLHDGTGISVSLHSERDKSMEESGFQIDF 120
           METSSTC+LVLSGKT AENETAKLLKRNDTLKL D   ISVSLHSERDKS++ESGFQI  
Sbjct: 61  METSSTCSLVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120

Query: 121 YMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180
           YM++LST+T+GRFLIWC RIPST DVIS NFSDLPLGAVCVADVQFKGRGRSKNLWESPP
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGRIVPLLQYVISLAITEA+KDICDK+GLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAVKDICDKEGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLSSTPYELRREDILAFFFNK 300
           VGGILCTSTYRSKKFNV+AGIGLNVDNDKPTTCLNEAL NLSSTPY+ RREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

Query: 301 FERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FE LYDIFINQGFQAL+ELYC+TWLHSGQRVIVQEKK DQVVENVVTIQGLT +GYLLAI
Sbjct: 301 FEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVENVVTIQGLTPTGYLLAI 360

Query: 361 GDDHQMCELHPDGNS 376
           GDD+QMCELHPDGNS
Sbjct: 361 GDDNQMCELHPDGNS 375

[Close]

BLAST of Lag0029272 vs. NCBI nr
Match: XP_022990857.1 (biotin--protein ligase 2-like [Cucurbita maxima])

HSP 1 Score: 648.3 bits (1671), Expect = 5.9e-182
Identity = 325/375 (86.67%), Postives = 340/375 (90.67%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIINSPSLRSLSKTTASAPTPETSINVREKRRLLSILSGSA 60
           M LTNPLR FS R SR   SII+SPS RS   TTASAPTP TS  V EK RL S+ SG A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLFSGLA 60

Query: 61  METSSTCALVLSGKTAAENETAKLLKRNDTLKLHDGTGISVSLHSERDKSMEESGFQIDF 120
           ME SSTCALVLSGKT AENETAKLLKRNDTLKL D   ISVSLHSERDKS++ESGFQI  
Sbjct: 61  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120

Query: 121 YMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180
           YM++LST+T+GRFLIWC RIPST DVIS NFSDLPLGAVCVADVQFKGRGRSKNLWESPP
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGRIVPLLQYVISLAITEA+KDICDKKGLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAVKDICDKKGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLSSTPYELRREDILAFFFNK 300
           VGGILCTSTYRSKKFNV+AGIGLNVDNDKPTTCLNEAL NLSSTPY+ RREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

Query: 301 FERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FE LYDIFINQGFQAL+ELYC+TWLHSGQRVIVQEKK DQVVENVVTIQGLT +GYLLAI
Sbjct: 301 FEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVENVVTIQGLTPTGYLLAI 360

Query: 361 GDDHQMCELHPDGNS 376
           GDD+QMCELHPDGNS
Sbjct: 361 GDDNQMCELHPDGNS 375

[Close]

BLAST of Lag0029272 vs. NCBI nr
Match: KAG6602073.1 (Serine/threonine-protein kinase MPS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 637.5 bits (1643), Expect = 1.0e-178
Identity = 317/363 (87.33%), Postives = 334/363 (92.01%), Query Frame = 0


Query: 13   RDSRYIFSIINSPSLRSLSKTTASAPTPETSINVREKRRLLSILSGSAMETSSTCALVLS 72
            R SR   SIINSPS RS   TTASAPTP TS  V EK RL S+LSG AMETSSTC+LVLS
Sbjct: 846  RGSRCFHSIINSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLLSGLAMETSSTCSLVLS 905

Query: 73   GKTAAENETAKLLKRNDTLKLHDGTGISVSLHSERDKSMEESGFQIDFYMDALSTNTYGR 132
            GKT AENETAKLLKRNDTLKL D   ISVSLHSERDKS++ESGFQI  YM++LST+T+GR
Sbjct: 906  GKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGSYMNSLSTDTFGR 965

Query: 133  FLIWCPRIPSTHDVISHNFSDLPLGAVCVADVQFKGRGRSKNLWESPPGCLMFSFTIQME 192
            FLIWC RIPST DVIS NFSDLPLGAVCVADVQFKGRGRSKNLWESPPGCLMFSFTIQME
Sbjct: 966  FLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPPGCLMFSFTIQME 1025

Query: 193  DGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLKVGGILCTSTYRS 252
            DGRIVPLLQYVISLAITEA+KDICDKKGLPYIDLKIKWPNDLYVNGLKVGGILCTSTYRS
Sbjct: 1026 DGRIVPLLQYVISLAITEAVKDICDKKGLPYIDLKIKWPNDLYVNGLKVGGILCTSTYRS 1085

Query: 253  KKFNVSAGIGLNVDNDKPTTCLNEALTNLSSTPYELRREDILAFFFNKFERLYDIFINQG 312
            KKFNV+AGIGLN+DNDKPTTCLNEAL NLSSTPY+ RREDIL+FFFNKFERLYDIFINQG
Sbjct: 1086 KKFNVTAGIGLNLDNDKPTTCLNEALANLSSTPYKFRREDILSFFFNKFERLYDIFINQG 1145

Query: 313  FQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAIGDDHQMCELHPD 372
            FQAL+ELYC+TWLHSGQRVIVQEKK DQVVENVVTIQGLT +GYLLAIGDD+QMCELHPD
Sbjct: 1146 FQALEELYCQTWLHSGQRVIVQEKKEDQVVENVVTIQGLTPTGYLLAIGDDNQMCELHPD 1205

Query: 373  GNS 376
            GNS
Sbjct: 1206 GNS 1208

[Close]

BLAST of Lag0029272 vs. NCBI nr
Match: KAA0050822.1 (biotin--protein ligase 2-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 637.1 bits (1642), Expect = 1.4e-178
Identity = 309/375 (82.40%), Postives = 343/375 (91.47%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIINSPSLRSLSKTTASAPTPETSINVREKRRLLSILSGSA 60
           M+L N LRYF RRDSR++FS++NS S  SLS+  AS PTP  SIN RE+RRL S+ S S 
Sbjct: 1   MLLANQLRYFGRRDSRFVFSVLNSSSFWSLSRIVASPPTPAISINERERRRLFSLSSASG 60

Query: 61  METSSTCALVLSGKTAAENETAKLLKRNDTLKLHDGTGISVSLHSERDKSMEESGFQIDF 120
           M+ S TC+LVLSGKTAAENETAKLLKRNDTLKL D T ISV LHSE+DK +EE+GF+ID 
Sbjct: 61  MDASPTCSLVLSGKTAAENETAKLLKRNDTLKLPDDTEISVLLHSEKDKPLEENGFRIDL 120

Query: 121 YMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180
           Y++ALST+T+GRFLIW PR+PST DVISHNFS+LPLGAVCVADVQFKGRGRSKNLWESPP
Sbjct: 121 YLNALSTDTFGRFLIWSPRVPSTQDVISHNFSNLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGR VPLLQYVISLAITEAIKDICDK+GLPYIDLKIKWPNDLYVN LK
Sbjct: 181 GCLMFSFTIQMEDGRTVPLLQYVISLAITEAIKDICDKEGLPYIDLKIKWPNDLYVNDLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLSSTPYELRREDILAFFFNK 300
           VGG+LCTSTYR KKFNV+AGIGLNV+NDKP+TCLNEALT+LSSTPY+ R+EDILAFFFNK
Sbjct: 241 VGGVLCTSTYRLKKFNVTAGIGLNVENDKPSTCLNEALTDLSSTPYKFRKEDILAFFFNK 300

Query: 301 FERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FERLYD+FINQGF+AL+ELY +TWLHSGQRV+VQEKK DQVVENVVTIQGLTSSGYLLAI
Sbjct: 301 FERLYDVFINQGFRALEELYYQTWLHSGQRVVVQEKKEDQVVENVVTIQGLTSSGYLLAI 360

Query: 361 GDDHQMCELHPDGNS 376
           GDD+QMCELHPDGNS
Sbjct: 361 GDDNQMCELHPDGNS 375

[Close]

BLAST of Lag0029272 vs. ExPASy Swiss-Prot
Match: Q9SL92 (Biotin--protein ligase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HCS1 PE=1 SV=2)

HSP 1 Score: 465.7 bits (1197), Expect = 7.1e-130
Identity = 215/333 (64.56%), Postives = 277/333 (83.18%), Query Frame = 0


Query: 45  NVREKRRLLSILSGSAMETSSTCALVLSGKTAAENETAKLLKRNDTLKLHDGTGISVSLH 104
           +++   RL    S SAME+ ++C+LVL GK++ E E AK LK  ++LKL D T +S+ L 
Sbjct: 23  SLKPLHRLSFSFSASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILE 82

Query: 105 SERDKSM--EESGFQIDFYMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLPLGAVCVA 164
           SE    +  +++ F +  +M+++ T+ +GRFLIW PR+ STHDV+SHNFS+LP+G+VCV 
Sbjct: 83  SEAKNLVKDDDNSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVT 142

Query: 165 DVQFKGRGRSKNLWESPPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLP 224
           D+QFKGRGR+KN+WESP GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGLP
Sbjct: 143 DIQFKGRGRTKNVWESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLP 202

Query: 225 YIDLKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLS 284
           YID+KIKWPNDLYVNGLKVGGILCTSTYRSKKFNVS G+GLNVDN +PTTCLN  L  ++
Sbjct: 203 YIDVKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMA 262

Query: 285 STPYELRREDILAFFFNKFERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVV 344
                L+RE+IL  FF+KFE+ +D+F++QGF++L+ELY RTWLHS QRVIV++K  DQVV
Sbjct: 263 PESNLLKREEILGAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVV 322

Query: 345 ENVVTIQGLTSSGYLLAIGDDHQMCELHPDGNS 376
           +NVVTIQGLTSSGYLLA+GDD+QM ELHPDGNS
Sbjct: 323 QNVVTIQGLTSSGYLLAVGDDNQMYELHPDGNS 355

[Close]

BLAST of Lag0029272 vs. ExPASy Swiss-Prot
Match: F4I4W2 (Biotin--protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=HCS2 PE=2 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 2.9e-123
Identity = 202/317 (63.72%), Postives = 262/317 (82.65%), Query Frame = 0


Query: 61  METSSTCALVLSGKTAAENETAKLLKRNDTLKLHDGTGISVSLHSERDKSM--EESGFQI 120
           M+  ++C+LVL GK++ E +TA  LK N+ LKL D + +S+ L SE    +  ++S F +
Sbjct: 1   MDIDASCSLVLYGKSSVETDTATRLKNNNVLKLPDNSKVSIFLQSEIKNLVRDDDSSFNL 60

Query: 121 DFYMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLPLGAVCVADVQFKGRGRSKNLWES 180
             +M+++ST+ +GRFLIW P + STHDV+SHNFS++P+G+VCV+D+Q KGRGR+KN+WES
Sbjct: 61  SLFMNSISTHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWES 120

Query: 181 PPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNG 240
           P GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGL Y D+KIKWPNDLY+NG
Sbjct: 121 PKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNG 180

Query: 241 LKVGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLSSTPYELRREDILAFFF 300
           LK+GGILCTSTYRS+KF VS G+GLNVDN++PTTCLN  L ++      L+RE+IL  FF
Sbjct: 181 LKIGGILCTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFF 240

Query: 301 NKFERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLL 360
            KFE  +D+F+ QGF++L+ELY RTWLHSGQRVI +EK  DQVV+NVVTIQGLTSSGYLL
Sbjct: 241 KKFENFFDLFMEQGFKSLEELYYRTWLHSGQRVIAEEKNEDQVVQNVVTIQGLTSSGYLL 300

Query: 361 AIGDDHQMCELHPDGNS 376
           AIGDD+ M ELHPDGNS
Sbjct: 301 AIGDDNVMYELHPDGNS 317

[Close]

BLAST of Lag0029272 vs. ExPASy Swiss-Prot
Match: P50747 (Biotin--protein ligase OS=Homo sapiens OX=9606 GN=HLCS PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 3.2e-37
Identity = 86/271 (31.73%), Postives = 150/271 (55.35%), Query Frame = 0


Query: 116 FQIDFYMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLP--LGAVCVADVQFKGRGRSK 175
           F ++ Y   L T   G+ +++    P+T  ++       P  +G + +A  Q +G+GR  
Sbjct: 451 FNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGG 510

Query: 176 NLWESPPGC----LMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIK 235
           N+W SP GC    L+ S  ++ + G+ +P +Q+++S+A+ EA++ I + +    I+L++K
Sbjct: 511 NVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQD---INLRVK 570

Query: 236 WPNDLYVNGL-KVGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLS----ST 295
           WPND+Y + L K+GG+L  ST   + F +  G G NV N  PT C+N+ +T  +    + 
Sbjct: 571 WPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAE 630

Query: 296 PYELRREDILAFFFNKFERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVEN 355
              LR + ++A      E+L   F ++G  ++  LY R W+HSGQ+V +   +G +    
Sbjct: 631 LKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPK---- 690

Query: 356 VVTIQGLTSSGYLLAIGDDHQMCELHPDGNS 376
            V+I GL  SG+L    +  ++  +HPDGNS
Sbjct: 691 -VSIVGLDDSGFLQVHQEGGEVVTVHPDGNS 713

[Close]

BLAST of Lag0029272 vs. ExPASy Swiss-Prot
Match: Q920N2 (Biotin--protein ligase OS=Mus musculus OX=10090 GN=Hlcs PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 2.7e-36
Identity = 86/271 (31.73%), Postives = 144/271 (53.14%), Query Frame = 0


Query: 116 FQIDFYMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLP--LGAVCVADVQFKGRGRSK 175
           F ++ Y   L T   G+ +++     +T  ++     ++P  +G + +A  Q +G+GR  
Sbjct: 447 FSLETYRQNLQTTRLGKVILFAEVTSTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGP 506

Query: 176 NLWESPPGC----LMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIK 235
           N W SP GC    L+    ++ + G+ +P +Q+++SLA+ EA++ I    G   I+L++K
Sbjct: 507 NAWLSPVGCALSTLLVFIPLRSQLGQRIPFVQHLMSLAVVEAVRSI---PGYEDINLRVK 566

Query: 236 WPNDLYVNGL-KVGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLSSTP--- 295
           WPND+Y + L K+GG+L  ST   + F +  G G NV N  PT C+N+ +   +      
Sbjct: 567 WPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLIEEHNKQHGAG 626

Query: 296 -YELRREDILAFFFNKFERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVEN 355
              LR + ++A      E+L D F +QG   +  LY + W+H GQ+V +   +G Q    
Sbjct: 627 LKPLRADCLIARAVTVLEKLIDRFQDQGPDGVLPLYYKYWVHGGQQVRLGSTEGPQ---- 686

Query: 356 VVTIQGLTSSGYLLAIGDDHQMCELHPDGNS 376
             +I GL  SG+L    +D  +  +HPDGNS
Sbjct: 687 -ASIVGLDDSGFLQVHQEDGGVVTVHPDGNS 709

[Close]

BLAST of Lag0029272 vs. ExPASy Swiss-Prot
Match: O14353 (Biotin--protein ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bpl1 PE=3 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 1.4e-29
Identity = 92/291 (31.62%), Postives = 144/291 (49.48%), Query Frame = 0


Query: 116 FQIDFYMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLP---LGAVCVADVQFKGRGRS 175
           F ++ Y   ++   +G  +I  P I ST  ++  N+  L     G   + + Q  GRGR 
Sbjct: 338 FDLELYAKLINGCRFGLPIIVAPVIRSTQTLLDKNYRFLDSTNTGFTVLGNYQTAGRGRG 397

Query: 176 KNLWESPPGCLMFSFTIQMEDGRI----VPLLQYVISLAITEAIKDICDKKGLPYIDLKI 235
           +N+W SP G L FSF I ++        + L QY+++LA+   I++     G   I   I
Sbjct: 398 QNMWVSPYGTLAFSFIINVDAKNFSTTPIALFQYLMALAVVRGIREYA--PGYENIPAFI 457

Query: 236 KWPNDLYV------------NGLKVGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNE 295
           KWPND+YV              +K+ GI+ TS YR    ++  G G+NV N  PT  LN 
Sbjct: 458 KWPNDVYVRVDKGGINFQGKQYMKLSGIIVTSNYRKNVLHLVVGCGINVSNLGPTVSLNT 517

Query: 296 ALT--NLSSTPYELRR---EDILAFFFNKFERLYDIFINQGFQALQELYCRTWLHSGQRV 355
            +   N +S    L +   E +LA   N+F+R + + + +GF  +   Y + WLHS Q V
Sbjct: 518 LVDEWNKNSDNPRLEKFSFEKLLASVLNQFDRYHRLLLEEGFSLILPEYYQYWLHSNQTV 577

Query: 356 IVQEKKGDQVVENVVTIQGLTSS-GYLLA--IGDDHQ----MCELHPDGNS 376
            +    G + +     IQG+TS  G+LLA  + ++++    +  L PDGNS
Sbjct: 578 NL--ASGGKAI-----IQGITSDFGFLLAQLLNENNEPTTKVVHLQPDGNS 619

[Close]

BLAST of Lag0029272 vs. ExPASy TrEMBL
Match: A0A6J1GYI6 (biotin--protein ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111458666 PE=3 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 4.4e-183
Identity = 326/375 (86.93%), Postives = 342/375 (91.20%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIINSPSLRSLSKTTASAPTPETSINVREKRRLLSILSGSA 60
           M LTNPLR FS R SR   SIINSPS RS   TTASAPTP TS  V EK RL S+LSG A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIINSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLLSGLA 60

Query: 61  METSSTCALVLSGKTAAENETAKLLKRNDTLKLHDGTGISVSLHSERDKSMEESGFQIDF 120
           METSSTC+LVLSGKT AENETAKLLKRNDTLKL D   ISVSLHSERDKS++ESGFQI  
Sbjct: 61  METSSTCSLVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120

Query: 121 YMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180
           YM++LST+T+GRFLIWC RIPST DVIS NFSDLPLGAVCVADVQFKGRGRSKNLWESPP
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGRIVPLLQYVISLAITEA+KDICDK+GLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAVKDICDKEGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLSSTPYELRREDILAFFFNK 300
           VGGILCTSTYRSKKFNV+AGIGLNVDNDKPTTCLNEAL NLSSTPY+ RREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

Query: 301 FERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FE LYDIFINQGFQAL+ELYC+TWLHSGQRVIVQEKK DQVVENVVTIQGLT +GYLLAI
Sbjct: 301 FEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVENVVTIQGLTPTGYLLAI 360

Query: 361 GDDHQMCELHPDGNS 376
           GDD+QMCELHPDGNS
Sbjct: 361 GDDNQMCELHPDGNS 375

[Close]

BLAST of Lag0029272 vs. ExPASy TrEMBL
Match: A0A6J1JK17 (biotin--protein ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487622 PE=3 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 2.9e-182
Identity = 325/375 (86.67%), Postives = 340/375 (90.67%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIINSPSLRSLSKTTASAPTPETSINVREKRRLLSILSGSA 60
           M LTNPLR FS R SR   SII+SPS RS   TTASAPTP TS  V EK RL S+ SG A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLFSGLA 60

Query: 61  METSSTCALVLSGKTAAENETAKLLKRNDTLKLHDGTGISVSLHSERDKSMEESGFQIDF 120
           ME SSTCALVLSGKT AENETAKLLKRNDTLKL D   ISVSLHSERDKS++ESGFQI  
Sbjct: 61  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120

Query: 121 YMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180
           YM++LST+T+GRFLIWC RIPST DVIS NFSDLPLGAVCVADVQFKGRGRSKNLWESPP
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGRIVPLLQYVISLAITEA+KDICDKKGLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAVKDICDKKGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLSSTPYELRREDILAFFFNK 300
           VGGILCTSTYRSKKFNV+AGIGLNVDNDKPTTCLNEAL NLSSTPY+ RREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

Query: 301 FERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FE LYDIFINQGFQAL+ELYC+TWLHSGQRVIVQEKK DQVVENVVTIQGLT +GYLLAI
Sbjct: 301 FEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVENVVTIQGLTPTGYLLAI 360

Query: 361 GDDHQMCELHPDGNS 376
           GDD+QMCELHPDGNS
Sbjct: 361 GDDNQMCELHPDGNS 375

[Close]

BLAST of Lag0029272 vs. ExPASy TrEMBL
Match: A0A5A7U4Q8 (Biotin--protein ligase 2-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold404G00810 PE=3 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 6.6e-179
Identity = 309/375 (82.40%), Postives = 343/375 (91.47%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIINSPSLRSLSKTTASAPTPETSINVREKRRLLSILSGSA 60
           M+L N LRYF RRDSR++FS++NS S  SLS+  AS PTP  SIN RE+RRL S+ S S 
Sbjct: 1   MLLANQLRYFGRRDSRFVFSVLNSSSFWSLSRIVASPPTPAISINERERRRLFSLSSASG 60

Query: 61  METSSTCALVLSGKTAAENETAKLLKRNDTLKLHDGTGISVSLHSERDKSMEESGFQIDF 120
           M+ S TC+LVLSGKTAAENETAKLLKRNDTLKL D T ISV LHSE+DK +EE+GF+ID 
Sbjct: 61  MDASPTCSLVLSGKTAAENETAKLLKRNDTLKLPDDTEISVLLHSEKDKPLEENGFRIDL 120

Query: 121 YMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180
           Y++ALST+T+GRFLIW PR+PST DVISHNFS+LPLGAVCVADVQFKGRGRSKNLWESPP
Sbjct: 121 YLNALSTDTFGRFLIWSPRVPSTQDVISHNFSNLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGR VPLLQYVISLAITEAIKDICDK+GLPYIDLKIKWPNDLYVN LK
Sbjct: 181 GCLMFSFTIQMEDGRTVPLLQYVISLAITEAIKDICDKEGLPYIDLKIKWPNDLYVNDLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLSSTPYELRREDILAFFFNK 300
           VGG+LCTSTYR KKFNV+AGIGLNV+NDKP+TCLNEALT+LSSTPY+ R+EDILAFFFNK
Sbjct: 241 VGGVLCTSTYRLKKFNVTAGIGLNVENDKPSTCLNEALTDLSSTPYKFRKEDILAFFFNK 300

Query: 301 FERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FERLYD+FINQGF+AL+ELY +TWLHSGQRV+VQEKK DQVVENVVTIQGLTSSGYLLAI
Sbjct: 301 FERLYDVFINQGFRALEELYYQTWLHSGQRVVVQEKKEDQVVENVVTIQGLTSSGYLLAI 360

Query: 361 GDDHQMCELHPDGNS 376
           GDD+QMCELHPDGNS
Sbjct: 361 GDDNQMCELHPDGNS 375

[Close]

BLAST of Lag0029272 vs. ExPASy TrEMBL
Match: A0A1S3BH31 (biotin--protein ligase 2-like isoform X4 OS=Cucumis melo OX=3656 GN=LOC103489963 PE=3 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 6.6e-179
Identity = 309/375 (82.40%), Postives = 343/375 (91.47%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIINSPSLRSLSKTTASAPTPETSINVREKRRLLSILSGSA 60
           M+L N LRYF RRDSR++FS++NS S  SLS+  AS PTP  SIN RE+RRL S+ S S 
Sbjct: 1   MLLANQLRYFGRRDSRFVFSVLNSSSFWSLSRIVASPPTPAISINERERRRLFSLSSASG 60

Query: 61  METSSTCALVLSGKTAAENETAKLLKRNDTLKLHDGTGISVSLHSERDKSMEESGFQIDF 120
           M+ S TC+LVLSGKTAAENETAKLLKRNDTLKL D T ISV LHSE+DK +EE+GF+ID 
Sbjct: 61  MDASPTCSLVLSGKTAAENETAKLLKRNDTLKLPDDTEISVLLHSEKDKPLEENGFRIDL 120

Query: 121 YMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180
           Y++ALST+T+GRFLIW PR+PST DVISHNFS+LPLGAVCVADVQFKGRGRSKNLWESPP
Sbjct: 121 YLNALSTDTFGRFLIWSPRVPSTQDVISHNFSNLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGR VPLLQYVISLAITEAIKDICDK+GLPYIDLKIKWPNDLYVN LK
Sbjct: 181 GCLMFSFTIQMEDGRTVPLLQYVISLAITEAIKDICDKEGLPYIDLKIKWPNDLYVNDLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLSSTPYELRREDILAFFFNK 300
           VGG+LCTSTYR KKFNV+AGIGLNV+NDKP+TCLNEALT+LSSTPY+ R+EDILAFFFNK
Sbjct: 241 VGGVLCTSTYRLKKFNVTAGIGLNVENDKPSTCLNEALTDLSSTPYKFRKEDILAFFFNK 300

Query: 301 FERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FERLYD+FINQGF+AL+ELY +TWLHSGQRV+VQEKK DQVVENVVTIQGLTSSGYLLAI
Sbjct: 301 FERLYDVFINQGFRALEELYYQTWLHSGQRVVVQEKKEDQVVENVVTIQGLTSSGYLLAI 360

Query: 361 GDDHQMCELHPDGNS 376
           GDD+QMCELHPDGNS
Sbjct: 361 GDDNQMCELHPDGNS 375

[Close]

BLAST of Lag0029272 vs. ExPASy TrEMBL
Match: A0A1S4DWM1 (biotin--protein ligase 2-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489963 PE=3 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 6.6e-179
Identity = 309/375 (82.40%), Postives = 343/375 (91.47%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIINSPSLRSLSKTTASAPTPETSINVREKRRLLSILSGSA 60
           M+L N LRYF RRDSR++FS++NS S  SLS+  AS PTP  SIN RE+RRL S+ S S 
Sbjct: 1   MLLANQLRYFGRRDSRFVFSVLNSSSFWSLSRIVASPPTPAISINERERRRLFSLSSASG 60

Query: 61  METSSTCALVLSGKTAAENETAKLLKRNDTLKLHDGTGISVSLHSERDKSMEESGFQIDF 120
           M+ S TC+LVLSGKTAAENETAKLLKRNDTLKL D T ISV LHSE+DK +EE+GF+ID 
Sbjct: 61  MDASPTCSLVLSGKTAAENETAKLLKRNDTLKLPDDTEISVLLHSEKDKPLEENGFRIDL 120

Query: 121 YMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180
           Y++ALST+T+GRFLIW PR+PST DVISHNFS+LPLGAVCVADVQFKGRGRSKNLWESPP
Sbjct: 121 YLNALSTDTFGRFLIWSPRVPSTQDVISHNFSNLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGR VPLLQYVISLAITEAIKDICDK+GLPYIDLKIKWPNDLYVN LK
Sbjct: 181 GCLMFSFTIQMEDGRTVPLLQYVISLAITEAIKDICDKEGLPYIDLKIKWPNDLYVNDLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLSSTPYELRREDILAFFFNK 300
           VGG+LCTSTYR KKFNV+AGIGLNV+NDKP+TCLNEALT+LSSTPY+ R+EDILAFFFNK
Sbjct: 241 VGGVLCTSTYRLKKFNVTAGIGLNVENDKPSTCLNEALTDLSSTPYKFRKEDILAFFFNK 300

Query: 301 FERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FERLYD+FINQGF+AL+ELY +TWLHSGQRV+VQEKK DQVVENVVTIQGLTSSGYLLAI
Sbjct: 301 FERLYDVFINQGFRALEELYYQTWLHSGQRVVVQEKKEDQVVENVVTIQGLTSSGYLLAI 360

Query: 361 GDDHQMCELHPDGNS 376
           GDD+QMCELHPDGNS
Sbjct: 361 GDDNQMCELHPDGNS 375

[Close]

BLAST of Lag0029272 vs. TAIR 10
Match: AT2G25710.1 (holocarboxylase synthase 1 )

HSP 1 Score: 465.7 bits (1197), Expect = 5.1e-131
Identity = 215/333 (64.56%), Postives = 277/333 (83.18%), Query Frame = 0


Query: 45  NVREKRRLLSILSGSAMETSSTCALVLSGKTAAENETAKLLKRNDTLKLHDGTGISVSLH 104
           +++   RL    S SAME+ ++C+LVL GK++ E E AK LK  ++LKL D T +S+ L 
Sbjct: 23  SLKPLHRLSFSFSASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILE 82

Query: 105 SERDKSM--EESGFQIDFYMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLPLGAVCVA 164
           SE    +  +++ F +  +M+++ T+ +GRFLIW PR+ STHDV+SHNFS+LP+G+VCV 
Sbjct: 83  SEAKNLVKDDDNSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVT 142

Query: 165 DVQFKGRGRSKNLWESPPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLP 224
           D+QFKGRGR+KN+WESP GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGLP
Sbjct: 143 DIQFKGRGRTKNVWESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLP 202

Query: 225 YIDLKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLS 284
           YID+KIKWPNDLYVNGLKVGGILCTSTYRSKKFNVS G+GLNVDN +PTTCLN  L  ++
Sbjct: 203 YIDVKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMA 262

Query: 285 STPYELRREDILAFFFNKFERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVV 344
                L+RE+IL  FF+KFE+ +D+F++QGF++L+ELY RTWLHS QRVIV++K  DQVV
Sbjct: 263 PESNLLKREEILGAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVV 322

Query: 345 ENVVTIQGLTSSGYLLAIGDDHQMCELHPDGNS 376
           +NVVTIQGLTSSGYLLA+GDD+QM ELHPDGNS
Sbjct: 323 QNVVTIQGLTSSGYLLAVGDDNQMYELHPDGNS 355

[Close]

BLAST of Lag0029272 vs. TAIR 10
Match: AT2G25710.2 (holocarboxylase synthase 1 )

HSP 1 Score: 465.7 bits (1197), Expect = 5.1e-131
Identity = 215/333 (64.56%), Postives = 277/333 (83.18%), Query Frame = 0


Query: 45  NVREKRRLLSILSGSAMETSSTCALVLSGKTAAENETAKLLKRNDTLKLHDGTGISVSLH 104
           +++   RL    S SAME+ ++C+LVL GK++ E E AK LK  ++LKL D T +S+ L 
Sbjct: 23  SLKPLHRLSFSFSASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILE 82

Query: 105 SERDKSM--EESGFQIDFYMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLPLGAVCVA 164
           SE    +  +++ F +  +M+++ T+ +GRFLIW PR+ STHDV+SHNFS+LP+G+VCV 
Sbjct: 83  SEAKNLVKDDDNSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVT 142

Query: 165 DVQFKGRGRSKNLWESPPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLP 224
           D+QFKGRGR+KN+WESP GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGLP
Sbjct: 143 DIQFKGRGRTKNVWESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLP 202

Query: 225 YIDLKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLS 284
           YID+KIKWPNDLYVNGLKVGGILCTSTYRSKKFNVS G+GLNVDN +PTTCLN  L  ++
Sbjct: 203 YIDVKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMA 262

Query: 285 STPYELRREDILAFFFNKFERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVV 344
                L+RE+IL  FF+KFE+ +D+F++QGF++L+ELY RTWLHS QRVIV++K  DQVV
Sbjct: 263 PESNLLKREEILGAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVV 322

Query: 345 ENVVTIQGLTSSGYLLAIGDDHQMCELHPDGNS 376
           +NVVTIQGLTSSGYLLA+GDD+QM ELHPDGNS
Sbjct: 323 QNVVTIQGLTSSGYLLAVGDDNQMYELHPDGNS 355

[Close]

BLAST of Lag0029272 vs. TAIR 10
Match: AT1G37150.2 (holocarboxylase synthetase 2 )

HSP 1 Score: 443.7 bits (1140), Expect = 2.1e-124
Identity = 202/317 (63.72%), Postives = 262/317 (82.65%), Query Frame = 0


Query: 61  METSSTCALVLSGKTAAENETAKLLKRNDTLKLHDGTGISVSLHSERDKSM--EESGFQI 120
           M+  ++C+LVL GK++ E +TA  LK N+ LKL D + +S+ L SE    +  ++S F +
Sbjct: 1   MDIDASCSLVLYGKSSVETDTATRLKNNNVLKLPDNSKVSIFLQSEIKNLVRDDDSSFNL 60

Query: 121 DFYMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLPLGAVCVADVQFKGRGRSKNLWES 180
             +M+++ST+ +GRFLIW P + STHDV+SHNFS++P+G+VCV+D+Q KGRGR+KN+WES
Sbjct: 61  SLFMNSISTHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWES 120

Query: 181 PPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNG 240
           P GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGL Y D+KIKWPNDLY+NG
Sbjct: 121 PKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNG 180

Query: 241 LKVGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLSSTPYELRREDILAFFF 300
           LK+GGILCTSTYRS+KF VS G+GLNVDN++PTTCLN  L ++      L+RE+IL  FF
Sbjct: 181 LKIGGILCTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFF 240

Query: 301 NKFERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLL 360
            KFE  +D+F+ QGF++L+ELY RTWLHSGQRVI +EK  DQVV+NVVTIQGLTSSGYLL
Sbjct: 241 KKFENFFDLFMEQGFKSLEELYYRTWLHSGQRVIAEEKNEDQVVQNVVTIQGLTSSGYLL 300

Query: 361 AIGDDHQMCELHPDGNS 376
           AIGDD+ M ELHPDGNS
Sbjct: 301 AIGDDNVMYELHPDGNS 317

[Close]

BLAST of Lag0029272 vs. TAIR 10
Match: AT1G37150.3 (holocarboxylase synthetase 2 )

HSP 1 Score: 396.7 bits (1018), Expect = 2.9e-110
Identity = 179/291 (61.51%), Postives = 238/291 (81.79%), Query Frame = 0


Query: 61  METSSTCALVLSGKTAAENETAKLLKRNDTLKLHDGTGISVSLHSERDKSM--EESGFQI 120
           M+  ++C+LVL GK++ E +TA  LK N+ LKL D + +S+ L SE    +  ++S F +
Sbjct: 1   MDIDASCSLVLYGKSSVETDTATRLKNNNVLKLPDNSKVSIFLQSEIKNLVRDDDSSFNL 60

Query: 121 DFYMDALSTNTYGRFLIWCPRIPSTHDVISHNFSDLPLGAVCVADVQFKGRGRSKNLWES 180
             +M+++ST+ +GRFLIW P + STHDV+SHNFS++P+G+VCV+D+Q KGRGR+KN+WES
Sbjct: 61  SLFMNSISTHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWES 120

Query: 181 PPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNG 240
           P GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGL Y D+KIKWPNDLY+NG
Sbjct: 121 PKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNG 180

Query: 241 LKVGGILCTSTYRSKKFNVSAGIGLNVDNDKPTTCLNEALTNLSSTPYELRREDILAFFF 300
           LK+GGILCTSTYRS+KF VS G+GLNVDN++PTTCLN  L ++      L+RE+IL  FF
Sbjct: 181 LKIGGILCTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFF 240

Query: 301 NKFERLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQ 350
            KFE  +D+F+ QGF++L+ELY RTWLHSGQRVI +EK  DQVV+NVVTIQ
Sbjct: 241 KKFENFFDLFMEQGFKSLEELYYRTWLHSGQRVIAEEKNEDQVVQNVVTIQ 291

[Close]

BLAST of Lag0029272 vs. TAIR 10
Match: AT1G37150.4 (holocarboxylase synthetase 2 )

HSP 1 Score: 382.9 bits (982), Expect = 4.3e-106
Identity = 170/238 (71.43%), Postives = 209/238 (87.82%), Query Frame = 0


Query: 138 PRIPSTHDVISHNFSDLPLGAVCVADVQFKGRGRSKNLWESPPGCLMFSFTIQMEDGRIV 197
           P + STHDV+SHNFS++P+G+VCV+D+Q KGRGR+KN+WESP GCLM+SFT++MEDGR+V
Sbjct: 37  PYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWESPKGCLMYSFTLEMEDGRVV 96

Query: 198 PLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLKVGGILCTSTYRSKKFNV 257
           PL+QYV+SLA+TEA+KD+CDKKGL Y D+KIKWPNDLY+NGLK+GGILCTSTYRS+KF V
Sbjct: 97  PLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNGLKIGGILCTSTYRSRKFLV 156

Query: 258 SAGIGLNVDNDKPTTCLNEALTNLSSTPYELRREDILAFFFNKFERLYDIFINQGFQALQ 317
           S G+GLNVDN++PTTCLN  L ++      L+RE+IL  FF KFE  +D+F+ QGF++L+
Sbjct: 157 SVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFFKKFENFFDLFMEQGFKSLE 216

Query: 318 ELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAIGDDHQMCELHPDGNS 376
           ELY RTWLHSGQRVI +EK  DQVV+NVVTIQGLTSSGYLLAIGDD+ M ELHPDGNS
Sbjct: 217 ELYYRTWLHSGQRVIAEEKNEDQVVQNVVTIQGLTSSGYLLAIGDDNVMYELHPDGNS 274

The following BLAST results are available for this feature:

BLAST of Lag0029272 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023517017.1	5.3e-183	86.67	biotin--protein ligase 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022957207.1	9.1e-183	86.93	biotin--protein ligase 2-like [Cucurbita moschata]	[more]
XP_022990857.1	5.9e-182	86.67	biotin--protein ligase 2-like [Cucurbita maxima]	[more]
KAG6602073.1	1.0e-178	87.33	Serine/threonine-protein kinase MPS1, partial [Cucurbita argyrosperma subsp. sor...	[more]
KAA0050822.1	1.4e-178	82.40	biotin--protein ligase 2-like isoform X2 [Cucumis melo var. makuwa]	[more]

BLAST of Lag0029272 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SL92	7.1e-130	64.56	Biotin--protein ligase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HCS1 ...	[more]
F4I4W2	2.9e-123	63.72	Biotin--protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=HCS2 PE=2 SV=1	[more]
P50747	3.2e-37	31.73	Biotin--protein ligase OS=Homo sapiens OX=9606 GN=HLCS PE=1 SV=1	[more]
Q920N2	2.7e-36	31.73	Biotin--protein ligase OS=Mus musculus OX=10090 GN=Hlcs PE=1 SV=1	[more]
O14353	1.4e-29	31.62	Biotin--protein ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX...	[more]

BLAST of Lag0029272 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GYI6	4.4e-183	86.93	biotin--protein ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111458666 PE=3...	[more]
A0A6J1JK17	2.9e-182	86.67	biotin--protein ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487622 PE=3 S...	[more]
A0A5A7U4Q8	6.6e-179	82.40	Biotin--protein ligase 2-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A1S3BH31	6.6e-179	82.40	biotin--protein ligase 2-like isoform X4 OS=Cucumis melo OX=3656 GN=LOC103489963...	[more]
A0A1S4DWM1	6.6e-179	82.40	biotin--protein ligase 2-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489963...	[more]

BLAST of Lag0029272 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G25710.1	5.1e-131	64.56	holocarboxylase synthase 1	[more]
AT2G25710.2	5.1e-131	64.56	holocarboxylase synthase 1	[more]
AT1G37150.2	2.1e-124	63.72	holocarboxylase synthetase 2	[more]
AT1G37150.3	2.9e-110	61.51	holocarboxylase synthetase 2	[more]
AT1G37150.4	4.3e-106	71.43	holocarboxylase synthetase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003142	Biotin protein ligase, C-terminal	PFAM	PF02237	BPL_C	coord: 326..375 e-value: 6.8E-7 score: 29.1
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 121..323 e-value: 1.2E-44 score: 153.9
None	No IPR available	PANTHER	PTHR12835	BIOTIN PROTEIN LIGASE	coord: 60..376
None	No IPR available	PANTHER	PTHR12835:SF5	HOLOCARBOXYLASE SYNTHETASE (BIOTIN-(PROPRIONYL-COA-CARBOXYLASE (ATP-HYDROLYSING)) LIGASE)	coord: 60..376
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 119..324
IPR004408	Biotin--acetyl-CoA-carboxylase ligase	TIGRFAM	TIGR00121	TIGR00121	coord: 137..359 e-value: 5.6E-36 score: 122.2
IPR004408	Biotin--acetyl-CoA-carboxylase ligase	CDD	cd16442	BPL	coord: 133..308 e-value: 1.74319E-51 score: 171.677
IPR004143	Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domain	PFAM	PF03099	BPL_LplA_LipB	coord: 138..264 e-value: 1.2E-29 score: 102.9
IPR004143	Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domain	PROSITE	PS51733	BPL_LPL_CATALYTIC	coord: 119..309 score: 28.527765

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0029272.1	Lag0029272.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006464	cellular protein modification process
molecular_function	GO:0004077	biotin-[acetyl-CoA-carboxylase] ligase activity