Lag0029250 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0029250
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	aminoacylase-1
Location	chr8: 36902062 .. 36904105 (+)
RNA-Seq Expression	Lag0029250
Synteny	Lag0029250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAACTTCCATCTCCACTATTCCCTCTTTTCACTCGCCACTTCTCTTCTTCTCCTCTCATTTCCTCTCTCTTTCAGCCATCCGCAGTACGGCGATGATGAAGCCGATCAAGATTCTCCAGTTTCTCGATTTCAGCACTACCTCAGGATCAAAACCGCTCATCCAAATCCAGATTACGCCTCCGCCGTCGCCTTCCTCAAATCTCAAGCACTGGCAATCGGTCTTCATGCGCAGATTCTCGAGTTCGTTCCAGGCAAGCCTCTTCTCCTCGTGACCTGGTATGGATCGAACCCTTCTTTGCCCTCGATTTTACTAAACTCTCACATGGACTCTGTCCCCGCCGAGCCCTCCAAGTGGCTCCATCCTCCATTCTCCGCCGTCCGGACCGCCGATGGCAAGATCTTCGCCCGTGGTTCTCAAGATGACAAGTGCATCGCCGTCCAGTACCTTGAAGCCATTAGGAACCTCAGAAGTCGGAATTTCGTCCCTGTCCGTACGATTCACATCTCGTATGTGCCGGATGAGGAGATTGGGGGTTTCGATGGAGCTGCGAAGTTCGTCTTGTCGAAGGAGTTTAAGGAGTTGAATGTGGGGTTCATGATGGACGAAGGACAGGCATCGCCGGAAGATGAGTTTAGGGTTTTTTACGCCGACCGATCGCCATGGGGGTTGATTATTAATGCTAAAGGAACCCCTGGGCATGGGTCTAGAATGTACGATAACAGTGCAATGGAGAATTTGATGAAGAGCGTTGAGATTGTCAGTAGGTTTAGAGAGAGTCAATTCGATATAGTGAAGGCCGGAGAAGCTGCTAATTCGGAAGTCATCTCTGTCAATCCTGTTTTCGTCAATGCCGGAATTCCTTCCCCCACCGTGAGTAGTCCGGTGAAATAAGTTGCAGGGTTTATGTACAGTAGAAAATCCCTCTGCCTGTGTCATTTTCGCCATTAATATTGTACTTTGTCTCAGGGATTCGTGATGAACATGCAACCTTCGGAGGCGGAGGCTGGATTTGATCTGAGAATACCGCCCACCGCCGACCCAGATTCTATAAGAAGAAGAATTTCCGAGGAATGGGCTCCAGCACGGCGGAACATGACGTTTCAGGTGAGTGGCTGCCTGATTTGATTCTGATATTCATCTGTTCTTTTGAGGTCCCCTGTTTTGTTAGCTCCCATTATTAGTATTTCTCTTGTCCAAGTTTTATGCTCTGTAATCTAAAGAAAAGCTTGGAGAAATCTTCTTAAAACTAAGATTATGATCTTAAACCAATTTTGATATGGAAAGAATTTGAAGTCTGAGTGAAGAGGATGTTGTTGCTTGTTCTACTCTATGAAACTCGTTTACTACACCAGGCTCTTTATTGGCGTACGAATTTTCCTTTGTCATTGTCAGGAGGAATTGACCAATTTTCCTCGTTTCCTCTCTGCATATCTGAGACCAAATGCCTGTTTTAGGCATGTCAAGCCTTGAGCAATCTTGTTTATTTTCTGTCTTTCAAGTTTCAAGGGTTAGATATTCAAGTATTGGCATCATATAGCAATCTCTTTTGAATTAAACTCTATCTGGTTGCTGAGACAGAATGGAACTGATTCCTGCAGATTATTCAGAAAGGGCCTATAAGAGATTACTTGGGACGTCCATTAATGACAATGACAAATGATTCAAATCCGTGGTGGTCGGTTTTCAAGACAGCCATCTCAGATGCTGGAGGCAAGCTTTCTAGGCCTGAAATCTTGGCTTCGACAACTGATGCACGCTTCATGAGACAGTTGGGCATTCCTGTTCTTGGTTTCTCCCCAATGACCAACACTCCCATCTTATTGCATGATCACAACGAGGTTGTACTAGTCTCCATTGTTATCTCTCTCTCCCTGTCAACGTTCTTGCTCGATTTGTGTTATGTTCTGACTAATGGGTCGTCTCATTTGTTTATATCTTGTTGCAGTTCTTGAAGGATACTGTCTTCTTAAGGGGCATAAAGGTTTATGAGTCCATAATCAGTGCTTTGAGTTCCTTTGAGGAGGATGCATCCAAATAA

mRNA sequence

ATGGCGAACTTCCATCTCCACTATTCCCTCTTTTCACTCGCCACTTCTCTTCTTCTCCTCTCATTTCCTCTCTCTTTCAGCCATCCGCAGTACGGCGATGATGAAGCCGATCAAGATTCTCCAGTTTCTCGATTTCAGCACTACCTCAGGATCAAAACCGCTCATCCAAATCCAGATTACGCCTCCGCCGTCGCCTTCCTCAAATCTCAAGCACTGGCAATCGGTCTTCATGCGCAGATTCTCGAGTTCGTTCCAGGCAAGCCTCTTCTCCTCGTGACCTGGTATGGATCGAACCCTTCTTTGCCCTCGATTTTACTAAACTCTCACATGGACTCTGTCCCCGCCGAGCCCTCCAAGTGGCTCCATCCTCCATTCTCCGCCGTCCGGACCGCCGATGGCAAGATCTTCGCCCGTGGTTCTCAAGATGACAAGTGCATCGCCGTCCAGTACCTTGAAGCCATTAGGAACCTCAGAAGTCGGAATTTCGTCCCTGTCCGTACGATTCACATCTCGTATGTGCCGGATGAGGAGATTGGGGGTTTCGATGGAGCTGCGAAGTTCGTCTTGTCGAAGGAGTTTAAGGAGTTGAATGTGGGGTTCATGATGGACGAAGGACAGGCATCGCCGGAAGATGAGTTTAGGGTTTTTTACGCCGACCGATCGCCATGGGGGTTGATTATTAATGCTAAAGGAACCCCTGGGCATGGGTCTAGAATGTACGATAACAGTGCAATGGAGAATTTGATGAAGAGCGTTGAGATTGTCAGTAGGTTTAGAGAGAGTCAATTCGATATAGTGAAGGCCGGAGAAGCTGCTAATTCGGAAGTCATCTCTGTCAATCCTGTTTTCGTCAATGCCGGAATTCCTTCCCCCACCGGATTCGTGATGAACATGCAACCTTCGGAGGCGGAGGCTGGATTTGATCTGAGAATACCGCCCACCGCCGACCCAGATTCTATAAGAAGAAGAATTTCCGAGGAATGGGCTCCAGCACGGCGGAACATGACGTTTCAGATTATTCAGAAAGGGCCTATAAGAGATTACTTGGGACGTCCATTAATGACAATGACAAATGATTCAAATCCGTGGTGGTCGGTTTTCAAGACAGCCATCTCAGATGCTGGAGGCAAGCTTTCTAGGCCTGAAATCTTGGCTTCGACAACTGATGCACGCTTCATGAGACAGTTGGGCATTCCTGTTCTTGGTTTCTCCCCAATGACCAACACTCCCATCTTATTGCATGATCACAACGAGTTCTTGAAGGATACTGTCTTCTTAAGGGGCATAAAGGTTTATGAGTCCATAATCAGTGCTTTGAGTTCCTTTGAGGAGGATGCATCCAAATAA

Coding sequence (CDS)

ATGGCGAACTTCCATCTCCACTATTCCCTCTTTTCACTCGCCACTTCTCTTCTTCTCCTCTCATTTCCTCTCTCTTTCAGCCATCCGCAGTACGGCGATGATGAAGCCGATCAAGATTCTCCAGTTTCTCGATTTCAGCACTACCTCAGGATCAAAACCGCTCATCCAAATCCAGATTACGCCTCCGCCGTCGCCTTCCTCAAATCTCAAGCACTGGCAATCGGTCTTCATGCGCAGATTCTCGAGTTCGTTCCAGGCAAGCCTCTTCTCCTCGTGACCTGGTATGGATCGAACCCTTCTTTGCCCTCGATTTTACTAAACTCTCACATGGACTCTGTCCCCGCCGAGCCCTCCAAGTGGCTCCATCCTCCATTCTCCGCCGTCCGGACCGCCGATGGCAAGATCTTCGCCCGTGGTTCTCAAGATGACAAGTGCATCGCCGTCCAGTACCTTGAAGCCATTAGGAACCTCAGAAGTCGGAATTTCGTCCCTGTCCGTACGATTCACATCTCGTATGTGCCGGATGAGGAGATTGGGGGTTTCGATGGAGCTGCGAAGTTCGTCTTGTCGAAGGAGTTTAAGGAGTTGAATGTGGGGTTCATGATGGACGAAGGACAGGCATCGCCGGAAGATGAGTTTAGGGTTTTTTACGCCGACCGATCGCCATGGGGGTTGATTATTAATGCTAAAGGAACCCCTGGGCATGGGTCTAGAATGTACGATAACAGTGCAATGGAGAATTTGATGAAGAGCGTTGAGATTGTCAGTAGGTTTAGAGAGAGTCAATTCGATATAGTGAAGGCCGGAGAAGCTGCTAATTCGGAAGTCATCTCTGTCAATCCTGTTTTCGTCAATGCCGGAATTCCTTCCCCCACCGGATTCGTGATGAACATGCAACCTTCGGAGGCGGAGGCTGGATTTGATCTGAGAATACCGCCCACCGCCGACCCAGATTCTATAAGAAGAAGAATTTCCGAGGAATGGGCTCCAGCACGGCGGAACATGACGTTTCAGATTATTCAGAAAGGGCCTATAAGAGATTACTTGGGACGTCCATTAATGACAATGACAAATGATTCAAATCCGTGGTGGTCGGTTTTCAAGACAGCCATCTCAGATGCTGGAGGCAAGCTTTCTAGGCCTGAAATCTTGGCTTCGACAACTGATGCACGCTTCATGAGACAGTTGGGCATTCCTGTTCTTGGTTTCTCCCCAATGACCAACACTCCCATCTTATTGCATGATCACAACGAGTTCTTGAAGGATACTGTCTTCTTAAGGGGCATAAAGGTTTATGAGTCCATAATCAGTGCTTTGAGTTCCTTTGAGGAGGATGCATCCAAATAA

Protein sequence

MANFHLHYSLFSLATSLLLLSFPLSFSHPQYGDDEADQDSPVSRFQHYLRIKTAHPNPDYASAVAFLKSQALAIGLHAQILEFVPGKPLLLVTWYGSNPSLPSILLNSHMDSVPAEPSKWLHPPFSAVRTADGKIFARGSQDDKCIAVQYLEAIRNLRSRNFVPVRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPEDEFRVFYADRSPWGLIINAKGTPGHGSRMYDNSAMENLMKSVEIVSRFRESQFDIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDSIRRRISEEWAPARRNMTFQIIQKGPIRDYLGRPLMTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMTNTPILLHDHNEFLKDTVFLRGIKVYESIISALSSFEEDASK

Homology

[Close]

BLAST of Lag0029250 vs. NCBI nr
Match: XP_038879728.1 (aminoacylase-1-like [Benincasa hispida])

HSP 1 Score: 828.9 bits (2140), Expect = 2.0e-236
Identity = 402/447 (89.93%), Postives = 428/447 (95.75%), Query Frame = 0


Query: 1   MANFHLHYSLFSLATSLLLLSFPLSFSHPQYGDDEADQDSPVSRFQHYLRIKTAHPNPDY 60
           MANFHLHY LF +A   LLLSFPLSFSH QYGD E DQDSP+SRFQ YLRIKTAHP+PDY
Sbjct: 1   MANFHLHYRLFPVAAFFLLLSFPLSFSHSQYGDGETDQDSPISRFQRYLRIKTAHPDPDY 60

Query: 61  ASAVAFLKSQALAIGLHAQILEFVPGKPLLLVTWYGSNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVAFL SQAL IGL+ +ILEFVPGKPLLLVTWYGSNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVAFLSSQALEIGLNTRILEFVPGKPLLLVTWYGSNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LHPPFSAVRTADGKIFARGSQDDKCIAVQYLEAIRNLRSRNFVPVRTIHISYVPDEEIGG 180
           LHPPFSAVRT+DG+IFARGSQDDKCIA+QYLEAIRNL+SRNF+PVRTIHISYVPDEEIGG
Sbjct: 121 LHPPFSAVRTSDGRIFARGSQDDKCIAIQYLEAIRNLKSRNFIPVRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPEDEFRVFYADRSPWGLIINAKGTPGHGSRMY 240
           FDGAAKFVLS+EFKELNVGFMMDEGQASP DEFRVFYADRSPWGLII AKGTPGHGSRMY
Sbjct: 181 FDGAAKFVLSQEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIKAKGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIVSRFRESQFDIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSA+ENLMKSVEIVSRFRESQF+IVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAIENLMKSVEIVSRFRESQFEIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDSIRRRISEEWAPARRNMTFQIIQKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLR+PPTADPD+IRRRI+EEWAPARRNMTFQII+KGPIRDYLG PLMTMTN S
Sbjct: 301 SEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQIIEKGPIRDYLGGPLMTMTNGS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMTNTPILLHDHNEFL 420
           NPWWSVF+TAIS+AGGKLS+PEILA+TTDARFMRQL IPVLGFSPM NTPILLHDHNEFL
Sbjct: 361 NPWWSVFRTAISEAGGKLSKPEILATTTDARFMRQLRIPVLGFSPMINTPILLHDHNEFL 420

Query: 421 KDTVFLRGIKVYESIISALSSFEEDAS 448
           +D+VFLRGIKVYES+ISALSSF++D S
Sbjct: 421 RDSVFLRGIKVYESLISALSSFQKDVS 447

[Close]

BLAST of Lag0029250 vs. NCBI nr
Match: XP_022964785.1 (aminoacylase-1 [Cucurbita moschata])

HSP 1 Score: 824.7 bits (2129), Expect = 3.8e-235
Identity = 403/448 (89.96%), Postives = 427/448 (95.31%), Query Frame = 0


Query: 1   MANFHLHYSLFSLATSLLLLSFPLSFSHPQYGDDEADQDSPVSRFQHYLRIKTAHPNPDY 60
           MANF LH  LFSLA  L L SFPLSFSH QY DDE+DQ+SP+SRFQHYLRIKTAHP+PDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVAFLKSQALAIGLHAQILEFVPGKPLLLVTWYGSNPSLPSILLNSHMDSVPAEPSKW 120
           ASAV+FL+SQAL IGLH +ILEFVPGKPLLLVTW G NPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LHPPFSAVRTADGKIFARGSQDDKCIAVQYLEAIRNLRSRNFVPVRTIHISYVPDEEIGG 180
           L+PPFSAVRT+DGKIFARGSQDDKCIA+QYLEAIR+LRSRNF+P RTIHISYVPDEEIGG
Sbjct: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPEDEFRVFYADRSPWGLIINAKGTPGHGSRMY 240
           FDGAAKFVLSKEFKELNVGFMMDEGQASP DEFRVFYADRSPWGL I A+GTPGHGSRMY
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIVSRFRESQFDIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEI+S+FRESQFD+VKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDSIRRRISEEWAPARRNMTFQIIQKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLRIPPTADPD+IRRRI EEWAPARRN+T QII+KGPIRDYLGRPL+TMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMTNTPILLHDHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPM+NTPILLH+HNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKVYESIISALSSFEEDASK 449
           KDTVFLRGIK+YESIISALSSF  DAS+
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASQ 448

[Close]

BLAST of Lag0029250 vs. NCBI nr
Match: KAG6602086.1 (Aminoacylase-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 823.5 bits (2126), Expect = 8.5e-235
Identity = 404/447 (90.38%), Postives = 425/447 (95.08%), Query Frame = 0


Query: 1   MANFHLHYSLFSLATSLLLLSFPLSFSHPQYGDDEADQDSPVSRFQHYLRIKTAHPNPDY 60
           MANF LH  LFSLA  L L SF LSFSH QY DDE+DQ+SP+SRFQHYLRIKTAHP+PDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFSFSLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVAFLKSQALAIGLHAQILEFVPGKPLLLVTWYGSNPSLPSILLNSHMDSVPAEPSKW 120
           ASAV+FL+SQAL IGLH +ILEFVPGKPLLLVTW G NPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LHPPFSAVRTADGKIFARGSQDDKCIAVQYLEAIRNLRSRNFVPVRTIHISYVPDEEIGG 180
           L+PPFSAVRT+DGKIFARGSQDDKCIA+QYLEAIRNLRSRNFVP RTIHISYVPDEEIGG
Sbjct: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPEDEFRVFYADRSPWGLIINAKGTPGHGSRMY 240
           FDGAAKFVLSKEFKELNVGFMMDEGQASP DEFRVFYADRSPWGLII A+GTPGHGSRMY
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIVSRFRESQFDIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEI+S+FRESQFD+VKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDSIRRRISEEWAPARRNMTFQIIQKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLRIPPTADPD+IRRRI EEWAPARRN+T QII+KGPIRDYLGRPL+TMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMTNTPILLHDHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQL IPVLGFSPM+NTPILLH+HNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKVYESIISALSSFEEDAS 448
           KDTVFLRGIK+YESIISALSSF  DAS
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADAS 447

[Close]

BLAST of Lag0029250 vs. NCBI nr
Match: KAG7032791.1 (Aminoacylase-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 823.5 bits (2126), Expect = 8.5e-235
Identity = 404/447 (90.38%), Postives = 425/447 (95.08%), Query Frame = 0


Query: 1   MANFHLHYSLFSLATSLLLLSFPLSFSHPQYGDDEADQDSPVSRFQHYLRIKTAHPNPDY 60
           MANF LH  LFSLA  L L SF LSFSH QY DDE+DQ+SP+SRFQHYLRIKTAHP+PDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFSFSLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVAFLKSQALAIGLHAQILEFVPGKPLLLVTWYGSNPSLPSILLNSHMDSVPAEPSKW 120
           ASAV+FL+SQAL IGLH +ILEFVPGKPLLLVTW G NPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LHPPFSAVRTADGKIFARGSQDDKCIAVQYLEAIRNLRSRNFVPVRTIHISYVPDEEIGG 180
           L+PPFSAVRT+DGKIFARGSQDDKCIA+QYLEAIRNLRSRNFVP RTIHISYVPDEEIGG
Sbjct: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPEDEFRVFYADRSPWGLIINAKGTPGHGSRMY 240
           FDGAAKFVLSKEFKELNVGFMMDEGQASP DEFRVFYADRSPWGLII A+GTPGHGSRMY
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIVSRFRESQFDIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEI+S+FRESQFD+VKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDSIRRRISEEWAPARRNMTFQIIQKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLRIPPTADPD+IRRRI EEWAPARRN+T QII+KGPIRDYLGRPL+TMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMTNTPILLHDHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQL IPVLGFSPM+NTPILLH+HNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKVYESIISALSSFEEDAS 448
           KDTVFLRGIK+YESIISALSSF  DAS
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADAS 447

[Close]

BLAST of Lag0029250 vs. NCBI nr
Match: XP_022991055.1 (aminoacylase-1 [Cucurbita maxima])

HSP 1 Score: 822.0 bits (2122), Expect = 2.5e-234
Identity = 401/448 (89.51%), Postives = 425/448 (94.87%), Query Frame = 0


Query: 1   MANFHLHYSLFSLATSLLLLSFPLSFSHPQYGDDEADQDSPVSRFQHYLRIKTAHPNPDY 60
           MANF LH  LFSLA  L L  FPLSFSH QY DDE+DQ+SP+SRFQHYLRIKTAHP+PDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVAFLKSQALAIGLHAQILEFVPGKPLLLVTWYGSNPSLPSILLNSHMDSVPAEPSKW 120
           ASAV+FL+SQAL IGLH +ILEFVPGKPLLLVTW G NPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LHPPFSAVRTADGKIFARGSQDDKCIAVQYLEAIRNLRSRNFVPVRTIHISYVPDEEIGG 180
           L+PPFSA+RT+DGKIFARGSQDDKCIA+QYLEAIRNLRSRNF+P RTIHISYVPDEEIGG
Sbjct: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPEDEFRVFYADRSPWGLIINAKGTPGHGSRMY 240
           FDGA+KFVLSKEFKELNVGFMMDEGQASP DEFRVFYADRSPWGLII A+G PGHGSRMY
Sbjct: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIVSRFRESQFDIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEI+S+FRESQFD+VKAGE ANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDSIRRRISEEWAPARRNMTFQIIQKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLRIPPTADPD+IRRRI EEWAPARRN+T QII+KGPIRDYLGRPLMTMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMTNTPILLHDHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPM+NTPILLH+HNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKVYESIISALSSFEEDASK 449
           KDTVFLRGIK+YESIISALSSF  DAS+
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASQ 448

[Close]

BLAST of Lag0029250 vs. ExPASy Swiss-Prot
Match: Q99JW2 (Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 8.1e-95
Identity = 178/410 (43.41%), Postives = 247/410 (60.24%), Query Frame = 0


Query: 33  DDEADQDSPVSRFQHYLRIKTAHPNPDYASAVAFLKSQALAIGLHAQILEFVPGKPLLLV 92
           D E++  S V+ F+ YLRI T  PNPDY  A+ FL+ +A  +GL  Q +E VPG  + ++
Sbjct: 5   DPESEHPS-VTLFRQYLRICTVQPNPDYGGAITFLEERARQLGLSCQKIEVVPGFVITVL 64

Query: 93  TWYGSNPSLPSILLNSHMDSVPAEPSKWLHPPFSAVRTADGKIFARGSQDDKCIAVQYLE 152
           TW G+NPSLPSILLNSH D VP     W H PF A + ++G I+ARGSQD K +++QYLE
Sbjct: 65  TWPGTNPSLPSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGSQDMKSVSIQYLE 124

Query: 153 AIRNLRSRNFVPVRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPEDE 212
           A+R L+S      RTIH+++VPDEE+GG  G   FV   EF+ L  GF +DEG A+P D 
Sbjct: 125 AVRRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDA 184

Query: 213 FRVFYADRSPWGLIINAKGTPGHGSRMYDNSAMENLMKSVEIVSRFRESQFDIVKAG-EA 272
           F VFY++RSPW + + + G PGH SR  +++A E L K +  +  FRE +   ++A    
Sbjct: 185 FTVFYSERSPWWVRVTSTGKPGHASRFIEDTAAEKLHKVISSILAFREKERQRLQANPHL 244

Query: 273 ANSEVISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDSIRRRISEEWAPA 332
               V SVN   +  G+        N+ P+   A FD R+ P  D  +  +++      A
Sbjct: 245 KEGAVTSVNLTKLEGGV------AYNVVPATMSASFDFRVAPDVDMKAFEKQLQRWCQEA 304

Query: 333 RRNMTFQIIQKGPIRDYLGRPLMTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDAR 392
              +TF+  QK         P MT T+DS+PWW+ F  A   A      PEI  + TD+R
Sbjct: 305 GEGVTFEFAQK------FTEPRMTPTDDSDPWWAAFSGACK-AMNLTLEPEIFPAATDSR 364

Query: 393 FMRQLGIPVLGFSPMTNTPILLHDHNEFLKDTVFLRGIKVYESIISALSS 442
           ++R +GIP LGFSPM  TP+LLHDHNE L + +FLRG+ +Y  ++SAL+S
Sbjct: 365 YIRAVGIPALGFSPMNRTPVLLHDHNERLHEDIFLRGVDIYTGLLSALAS 400

[Close]

BLAST of Lag0029250 vs. ExPASy Swiss-Prot
Match: P37111 (Aminoacylase-1 OS=Sus scrofa OX=9823 GN=ACY1 PE=1 SV=2)

HSP 1 Score: 345.9 bits (886), Expect = 6.9e-94
Identity = 172/400 (43.00%), Postives = 242/400 (60.50%), Query Frame = 0


Query: 42  VSRFQHYLRIKTAHPNPDYASAVAFLKSQALAIGLHAQILEFVPGKPLLLVTWYGSNPSL 101
           V+ F+ YLRI+T  P PDY +AVAFL+ +A  +GL  Q +E VPG  + ++TW G+NP+L
Sbjct: 13  VTLFRQYLRIRTVQPEPDYGAAVAFLEERARQLGLGCQKVEVVPGHVVTVLTWPGTNPTL 72

Query: 102 PSILLNSHMDSVPAEPSKWLHPPFSAVRTADGKIFARGSQDDKCIAVQYLEAIRNLRSRN 161
            SILLNSH D VP     W H PF   + ADG I+ RG+QD KC+++QYLEA+R L+   
Sbjct: 73  SSILLNSHTDVVPVFKEHWSHDPFEGFKDADGYIYGRGAQDMKCVSIQYLEAVRRLKVEG 132

Query: 162 FVPVRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPEDEFRVFYADRS 221
               RTIH+++VPDEE+GG  G   FV   EF+ L  GF +DEG ASP D F VFY++RS
Sbjct: 133 HHFPRTIHMTFVPDEEVGGHQGMELFVKRPEFQALRAGFALDEGLASPTDAFTVFYSERS 192

Query: 222 PWGLIINAKGTPGHGSRMYDNSAMENLMKSVEIVSRFRESQFDIVKAGEAANSEVISVNP 281
           PW L + + G PGHGSR  +++A E L K +  +  FRE +   +++ +     V SVN 
Sbjct: 193 PWWLRVTSTGKPGHGSRFIEDTAAEKLHKVINSILAFREKEKQRLQSNQLKPGAVTSVNL 252

Query: 282 VFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDSIRRRISEEWAPARRNMTFQIIQ 341
             +  G+        N+ P+   A FD R+ P  D  +   ++      A   +TF+ +Q
Sbjct: 253 TMLEGGV------AYNVVPATMSACFDFRVAPDVDLKAFEEQLQSWCQAAGEGVTFEFVQ 312

Query: 342 KGPIRDYLGRPLMTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVL 401
           K           +T T+DS+PWW+ F     D    L   EI  ++TDAR++R  G+P L
Sbjct: 313 KWM------ETQVTSTDDSDPWWAAFSGVFKDMKLALEL-EICPASTDARYIRAAGVPAL 372

Query: 402 GFSPMTNTPILLHDHNEFLKDTVFLRGIKVYESIISALSS 442
           GFSPM +TP+LLHDH+E L + VFLRG+ +Y  ++SAL+S
Sbjct: 373 GFSPMNHTPVLLHDHDERLHEAVFLRGVDIYTQLLSALAS 399

[Close]

BLAST of Lag0029250 vs. ExPASy Swiss-Prot
Match: Q6AYS7 (Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 2.2e-92
Identity = 173/401 (43.14%), Postives = 241/401 (60.10%), Query Frame = 0


Query: 42  VSRFQHYLRIKTAHPNPDYASAVAFLKSQALAIGLHAQILEFVPGKPLLLVTWYGSNPSL 101
           V+ F+ YLRI T  PNPDY SAV FL+ +A  +GL  Q +E  PG  + ++TW G+NP L
Sbjct: 13  VTLFRQYLRICTVQPNPDYGSAVTFLEERARQLGLSCQKIEVAPGYVITVLTWPGTNPLL 72

Query: 102 PSILLNSHMDSVPAEPSKWLHPPFSAVRTADGKIFARGSQDDKCIAVQYLEAIRNLRSRN 161
            SILLNSH D VP     W H PF A + ++G I+ARG+QD K +++QYLEA+R L+S  
Sbjct: 73  HSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGAQDMKSVSIQYLEAVRRLKSEG 132

Query: 162 FVPVRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPEDEFRVFYADRS 221
               RTIH+++VPDEE+GG  G   FV   EF+ L  GF +DEG A+P D F VFY++RS
Sbjct: 133 HRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFTVFYSERS 192

Query: 222 PWGLIINAKGTPGHGSRMYDNSAMENLMKSVEIVSRFRESQFDIVKAG-EAANSEVISVN 281
           PW + + + G PGH SR  +++A E L K V  +  FRE +   ++A        V SVN
Sbjct: 193 PWWIRVTSTGKPGHASRFIEDTAAEKLHKVVNSILAFREKERQRLQANPHLKEGAVTSVN 252

Query: 282 PVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDSIRRRISEEWAPARRNMTFQII 341
              +  G+        N+ P+   A FD R+ P  D  +  +++      A   +TF+  
Sbjct: 253 LTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEKQLQSWCQEAGEGVTFEFA 312

Query: 342 QKGPIRDYLGRPLMTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPV 401
           QK         P MT T+D++PWW+ F  A  +    L  PEI  + TD+R++R +GIP 
Sbjct: 313 QK------FTEPRMTPTDDTDPWWAAFSGACKEMNLTL-EPEIFPAATDSRYIRAVGIPA 372

Query: 402 LGFSPMTNTPILLHDHNEFLKDTVFLRGIKVYESIISALSS 442
           LGFSPM  TP+LLHDHNE L + VFLRG+ +Y  +++AL+S
Sbjct: 373 LGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALAS 400

[Close]

BLAST of Lag0029250 vs. ExPASy Swiss-Prot
Match: Q6PTT0 (Aminoacylase-1B OS=Rattus norvegicus OX=10116 GN=Acy1b PE=1 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 4.2e-91
Identity = 168/406 (41.38%), Postives = 241/406 (59.36%), Query Frame = 0


Query: 37  DQDSPVSRFQHYLRIKTAHPNPDYASAVAFLKSQALAIGLHAQILEFVPGKPLLLVTWYG 96
           ++   V+ F+ YLRI+T  P PDY +AVAF +  A  +GL  Q +E  PG  + ++TW G
Sbjct: 8   EEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVTVLTWPG 67

Query: 97  SNPSLPSILLNSHMDSVPAEPSKWLHPPFSAVRTADGKIFARGSQDDKCIAVQYLEAIRN 156
           +NP+L SILLNSH D VP     W H PF A + ++G I+ RG+QD KC+++QYLEA++ 
Sbjct: 68  TNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYTRGAQDMKCVSIQYLEAVKR 127

Query: 157 LRSRNFVPVRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPEDEFRVF 216
           L+       RTIH+++VPDEE+GG  G   FV   EF  L  GF +DEG A+P D F VF
Sbjct: 128 LKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRHEFHALRAGFALDEGLANPTDAFTVF 187

Query: 217 YADRSPWGLIINAKGTPGHGSRMYDNSAMENLMKSVEIVSRFRESQFDIVKAG-EAANSE 276
           Y++RSPW + + + G PGH SR  +++A E L K V  +  FRE ++  +++        
Sbjct: 188 YSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNPHLKEGS 247

Query: 277 VISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDSIRRRISEEWAPARRNM 336
           V SVN   +  G+        N+ P+   A FD R+ P  D  +   ++      A   +
Sbjct: 248 VTSVNLTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEEQLQSWCQEAGEGV 307

Query: 337 TFQIIQKGPIRDYLGRPLMTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQ 396
           TF+  QK         P MT T+D++PWW+ F  A  +    L  PEI  + TD+R++R 
Sbjct: 308 TFEFAQK------FTEPRMTPTDDTDPWWAAFSGACKEMTLTL-EPEIFPAATDSRYIRA 367

Query: 397 LGIPVLGFSPMTNTPILLHDHNEFLKDTVFLRGIKVYESIISALSS 442
           +GIP LGFSPM  TP+LLHDHNE L + VFLRG+ +Y  +++AL+S
Sbjct: 368 VGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALAS 400

[Close]

BLAST of Lag0029250 vs. ExPASy Swiss-Prot
Match: Q5RFB0 (Aminoacylase-1 OS=Pongo abelii OX=9601 GN=ACY1 PE=2 SV=2)

HSP 1 Score: 335.5 bits (859), Expect = 9.3e-91
Identity = 170/406 (41.87%), Postives = 240/406 (59.11%), Query Frame = 0


Query: 37  DQDSPVSRFQHYLRIKTAHPNPDYASAVAFLKSQALAIGLHAQILEFVPGKPLLLVTWYG 96
           ++   V+ F+ YLRI+T  P PDY +AVAF + +A  +GL  Q +E  PG  + ++TW G
Sbjct: 8   EEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEERARQLGLGCQKVEVAPGYVVTVLTWPG 67

Query: 97  SNPSLPSILLNSHMDSVPAEPSKWLHPPFSAVRTADGKIFARGSQDDKCIAVQYLEAIRN 156
           +NP+L SILLNSH D VP     W H PF A + ++G I+ARG+QD KCI++QYLEA+R 
Sbjct: 68  TNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCISIQYLEAVRR 127

Query: 157 LRSRNFVPVRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPEDEFRVF 216
           L+       RTIH+++VPDEE+GG  G   FV   EF  L  GF +DEG A+P D F VF
Sbjct: 128 LKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPTDAFTVF 187

Query: 217 YADRSPWGLIINAKGTPGHGSRMYDNSAMENLMKSVEIVSRFRESQFDIVKAG-EAANSE 276
           Y++RSPW + + + G PGH SR  +++A E L K V  +  FRE ++  +++        
Sbjct: 188 YSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVSSILAFREKEWQRLQSNPHLKEGS 247

Query: 277 VISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDSIRRRISEEWAPARRNM 336
           V SVN   +  G+        N+ P+   A FD R+ P  D  +   ++      A   +
Sbjct: 248 VTSVNLTKLEGGV------AYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWCQAAGEGV 307

Query: 337 TFQIIQKGPIRDYLGRPLMTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQ 396
           T +  QK         P +T T+DSNPWW+ F     D    L  PEI+ + TD R++R 
Sbjct: 308 TLEFAQKWM------HPQVTPTDDSNPWWAAFSRVCKDMKLTL-EPEIMPAATDNRYIRA 367

Query: 397 LGIPVLGFSPMTNTPILLHDHNEFLKDTVFLRGIKVYESIISALSS 442
           +GIP LGFSPM  TP+LLHDH+E L + VFLRG+ +Y  ++ AL+S
Sbjct: 368 VGIPALGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALAS 400

[Close]

BLAST of Lag0029250 vs. ExPASy TrEMBL
Match: A0A6J1HLT6 (aminoacylase-1 OS=Cucurbita moschata OX=3662 GN=LOC111464784 PE=4 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 1.9e-235
Identity = 403/448 (89.96%), Postives = 427/448 (95.31%), Query Frame = 0


Query: 1   MANFHLHYSLFSLATSLLLLSFPLSFSHPQYGDDEADQDSPVSRFQHYLRIKTAHPNPDY 60
           MANF LH  LFSLA  L L SFPLSFSH QY DDE+DQ+SP+SRFQHYLRIKTAHP+PDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVAFLKSQALAIGLHAQILEFVPGKPLLLVTWYGSNPSLPSILLNSHMDSVPAEPSKW 120
           ASAV+FL+SQAL IGLH +ILEFVPGKPLLLVTW G NPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LHPPFSAVRTADGKIFARGSQDDKCIAVQYLEAIRNLRSRNFVPVRTIHISYVPDEEIGG 180
           L+PPFSAVRT+DGKIFARGSQDDKCIA+QYLEAIR+LRSRNF+P RTIHISYVPDEEIGG
Sbjct: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPEDEFRVFYADRSPWGLIINAKGTPGHGSRMY 240
           FDGAAKFVLSKEFKELNVGFMMDEGQASP DEFRVFYADRSPWGL I A+GTPGHGSRMY
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIVSRFRESQFDIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEI+S+FRESQFD+VKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDSIRRRISEEWAPARRNMTFQIIQKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLRIPPTADPD+IRRRI EEWAPARRN+T QII+KGPIRDYLGRPL+TMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMTNTPILLHDHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPM+NTPILLH+HNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKVYESIISALSSFEEDASK 449
           KDTVFLRGIK+YESIISALSSF  DAS+
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASQ 448

[Close]

BLAST of Lag0029250 vs. ExPASy TrEMBL
Match: A0A6J1JRT3 (aminoacylase-1 OS=Cucurbita maxima OX=3661 GN=LOC111487760 PE=4 SV=1)

HSP 1 Score: 822.0 bits (2122), Expect = 1.2e-234
Identity = 401/448 (89.51%), Postives = 425/448 (94.87%), Query Frame = 0


Query: 1   MANFHLHYSLFSLATSLLLLSFPLSFSHPQYGDDEADQDSPVSRFQHYLRIKTAHPNPDY 60
           MANF LH  LFSLA  L L  FPLSFSH QY DDE+DQ+SP+SRFQHYLRIKTAHP+PDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVAFLKSQALAIGLHAQILEFVPGKPLLLVTWYGSNPSLPSILLNSHMDSVPAEPSKW 120
           ASAV+FL+SQAL IGLH +ILEFVPGKPLLLVTW G NPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LHPPFSAVRTADGKIFARGSQDDKCIAVQYLEAIRNLRSRNFVPVRTIHISYVPDEEIGG 180
           L+PPFSA+RT+DGKIFARGSQDDKCIA+QYLEAIRNLRSRNF+P RTIHISYVPDEEIGG
Sbjct: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPEDEFRVFYADRSPWGLIINAKGTPGHGSRMY 240
           FDGA+KFVLSKEFKELNVGFMMDEGQASP DEFRVFYADRSPWGLII A+G PGHGSRMY
Sbjct: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIVSRFRESQFDIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEI+S+FRESQFD+VKAGE ANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDSIRRRISEEWAPARRNMTFQIIQKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLRIPPTADPD+IRRRI EEWAPARRN+T QII+KGPIRDYLGRPLMTMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMTNTPILLHDHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPM+NTPILLH+HNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKVYESIISALSSFEEDASK 449
           KDTVFLRGIK+YESIISALSSF  DAS+
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASQ 448

[Close]

BLAST of Lag0029250 vs. ExPASy TrEMBL
Match: A0A5D3BIQ8 (Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001910 PE=4 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 1.8e-230
Identity = 390/447 (87.25%), Postives = 420/447 (93.96%), Query Frame = 0


Query: 1   MANFHLHYSLFSLATSLLLLSFPLSFSHPQYGDDEADQDSPVSRFQHYLRIKTAHPNPDY 60
           M+NF LH+  FS+   LLLLSFPLSFSH QY DDE+DQDSPVSRFQ YLRIKTAHP+PDY
Sbjct: 1   MSNFRLHHRFFSVTLYLLLLSFPLSFSHSQYVDDESDQDSPVSRFQRYLRIKTAHPDPDY 60

Query: 61  ASAVAFLKSQALAIGLHAQILEFVPGKPLLLVTWYGSNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVAFL+SQA  IGLH ++LEF PGKPLLLVTWYGSNPSLPS+LLNSHMDSVPAEPSKW
Sbjct: 61  ASAVAFLRSQAQEIGLHTEVLEFGPGKPLLLVTWYGSNPSLPSVLLNSHMDSVPAEPSKW 120

Query: 121 LHPPFSAVRTADGKIFARGSQDDKCIAVQYLEAIRNLRSRNFVPVRTIHISYVPDEEIGG 180
           LHPPFSAVRT+DGKIFARGSQDDKCIA+QYLEAIRNLR+R+FVPVRT+HISYVPDEEIGG
Sbjct: 121 LHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTVHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPEDEFRVFYADRSPWGLIINAKGTPGHGSRMY 240
            DG AKFV SKEFKELNVGFMMDEGQASP DEFRVFYADRSPW LII AKGTPGHGSRMY
Sbjct: 181 SDGVAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIVSRFRESQFDIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIVSRFRESQF+IVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIVSRFRESQFEIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDSIRRRISEEWAPARRNMTFQIIQKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLR+PPTADPD++RRRI+EEWAPA RNMT+QII+KGP+RDYLG PLMTMTNDS
Sbjct: 301 SEAEAGFDLRLPPTADPDAMRRRIAEEWAPAWRNMTYQIIEKGPLRDYLGSPLMTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMTNTPILLHDHNEFL 420
           NPWW+VFK AISDAGGKLS+PEILA+TTDARFMRQ+ IPVLGFSPM NTPILLHDHNEFL
Sbjct: 361 NPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNEFL 420

Query: 421 KDTVFLRGIKVYESIISALSSFEEDAS 448
            D+VF+RGIKVYES+IS LSSF+ED S
Sbjct: 421 SDSVFIRGIKVYESLISTLSSFQEDVS 447

[Close]

BLAST of Lag0029250 vs. ExPASy TrEMBL
Match: A0A5A7URV3 (Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G001820 PE=4 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 3.1e-230
Identity = 388/447 (86.80%), Postives = 421/447 (94.18%), Query Frame = 0


Query: 1   MANFHLHYSLFSLATSLLLLSFPLSFSHPQYGDDEADQDSPVSRFQHYLRIKTAHPNPDY 60
           M+NF LH+  FS+   LLLLSFPLSFSH QY DDE+DQDSPVSRFQ YLRIKTAHP+PDY
Sbjct: 85  MSNFRLHHRFFSVTLYLLLLSFPLSFSHSQYVDDESDQDSPVSRFQRYLRIKTAHPDPDY 144

Query: 61  ASAVAFLKSQALAIGLHAQILEFVPGKPLLLVTWYGSNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVAFL+SQA  IGLH ++LEF PGKPLLLVTWYGSNPSLPS+LLNSHMDSVPAEPSKW
Sbjct: 145 ASAVAFLRSQAQEIGLHTEVLEFGPGKPLLLVTWYGSNPSLPSVLLNSHMDSVPAEPSKW 204

Query: 121 LHPPFSAVRTADGKIFARGSQDDKCIAVQYLEAIRNLRSRNFVPVRTIHISYVPDEEIGG 180
           LHPPFSAVRT+DGKIFARGSQDDKCIA+QY+EAIRNLR+R+FVPVRT+HISYVPDEEIGG
Sbjct: 205 LHPPFSAVRTSDGKIFARGSQDDKCIAIQYIEAIRNLRNRDFVPVRTVHISYVPDEEIGG 264

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPEDEFRVFYADRSPWGLIINAKGTPGHGSRMY 240
            DG AKFV SKEFKELNVGFMMDEGQASP DEFRVFYADRSPW LII AKGTPGHGSRMY
Sbjct: 265 SDGVAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGTPGHGSRMY 324

Query: 241 DNSAMENLMKSVEIVSRFRESQFDIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEI+SRFRESQF+IVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 325 DNSAMENLMKSVEIISRFRESQFEIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 384

Query: 301 SEAEAGFDLRIPPTADPDSIRRRISEEWAPARRNMTFQIIQKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLR+PPTADPD++RRRI+EEWAPA RNMT+QII+KGP+RDYLG PLMTMTNDS
Sbjct: 385 SEAEAGFDLRLPPTADPDAMRRRIAEEWAPAWRNMTYQIIEKGPLRDYLGSPLMTMTNDS 444

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMTNTPILLHDHNEFL 420
           NPWW+VFK AISDAGGKLS+PEILA+TTDARFMRQ+ IPVLGFSPM NTPILLHDHNEFL
Sbjct: 445 NPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNEFL 504

Query: 421 KDTVFLRGIKVYESIISALSSFEEDAS 448
            D+VF+RGIKVYES+IS+LSSF+ED S
Sbjct: 505 SDSVFIRGIKVYESLISSLSSFQEDVS 531

[Close]

BLAST of Lag0029250 vs. ExPASy TrEMBL
Match: A0A6J1CK37 (aminoacylase-1 OS=Momordica charantia OX=3673 GN=LOC111011881 PE=4 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 4.4e-229
Identity = 401/448 (89.51%), Postives = 418/448 (93.30%), Query Frame = 0


Query: 1   MANFHLHYSLFSLATSLLLLSFPLSFSHPQYGDDEADQDSPVSRFQHYLRIKTAHPNPDY 60
           MAN H H S  SLA  LLLLSFPLSF H Q  DDE D DSPVSRFQ+YLRIKTAHPNPDY
Sbjct: 1   MANLHHHCSFISLAAFLLLLSFPLSFCHLQ--DDEVDPDSPVSRFQNYLRIKTAHPNPDY 60

Query: 61  ASAVAFLKSQALAIGLHAQILEFVPGKPLLLVTWYGSNPSLPSILLNSHMDSVPAEPSKW 120
           A AV+FLKSQALAIGL AQILEFV GKPLLL+TW GSNPSLPSILLNSHMDSVPAE SKW
Sbjct: 61  AFAVSFLKSQALAIGLDAQILEFVEGKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKW 120

Query: 121 LHPPFSAVRTADGKIFARGSQDDKCIAVQYLEAIRNLRSRNFVPVRTIHISYVPDEEIGG 180
           LHPPFSAVR++DGKIFARGSQDDKCIA+QYLEAIRNLRSRNFVPVR+IHISYVPDEEIGG
Sbjct: 121 LHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPEDEFRVFYADRSPWGLIINAKGTPGHGSRMY 240
           FDGAAKFV SKEFKELNVGFMMDEGQASP DEFRVFYADRSPWGLII A GTPGHGS +Y
Sbjct: 181 FDGAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLY 240

Query: 241 DNSAMENLMKSVEIVSRFRESQFDIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIVSRFRESQFD+VKAGEA NSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDSIRRRISEEWAPARRNMTFQIIQKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLR+PPTADPD+IRRRI+EEWAPARRNMTFQI Q+GPIRDYLGRPLMTMTN S
Sbjct: 301 SEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMTNTPILLHDHNEFL 420
           N WWSVF+TAISDAGGKLS PEILASTTDARFMRQLGIPVLGFSPM+NTPILLHDHNEFL
Sbjct: 361 NRWWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFL 420

Query: 421 KDTVFLRGIKVYESIISALSSFEEDASK 449
            +TVFLRGIKVYESIISALSSFEE AS+
Sbjct: 421 SETVFLRGIKVYESIISALSSFEEGASQ 446

[Close]

BLAST of Lag0029250 vs. TAIR 10
Match: AT1G44820.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 639.0 bits (1647), Expect = 2.8e-183
Identity = 295/430 (68.60%), Postives = 364/430 (84.65%), Query Frame = 0


Query: 19  LLSFPLSFSHPQYGDDEADQDSPVSRFQHYLRIKTAHPNPDYASAVAFLKSQALAIGLHA 78
           L+ F + FS        +++D+P++RFQ YLR  TAHPNP+Y + ++FL +QA +IGL  
Sbjct: 10  LIIFSIIFS---LQSSSSEEDTPITRFQQYLRFNTAHPNPNYTAPISFLINQAQSIGLTT 69

Query: 79  QILEFVPGKPLLLVTWYGSNPSLPSILLNSHMDSVPAEPSKWLHPPFSAVRTADGKIFAR 138
           + +EF+ GKP+LL+TW GSNP+LPSIL NSH+DSVPAE  KW +PPFSA +T DG I+AR
Sbjct: 70  KTIEFISGKPILLITWLGSNPNLPSILFNSHLDSVPAESEKWTYPPFSAHKTIDGHIYAR 129

Query: 139 GSQDDKCIAVQYLEAIRNLRSRNFVPVRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNV 198
           G+QDDKCI VQYLE+IRNL+SR F P+RTIHISYVP+EEIGGFDG  KF  S EFK+LN+
Sbjct: 130 GAQDDKCIGVQYLESIRNLKSRGFSPLRTIHISYVPEEEIGGFDGMMKFAASSEFKDLNL 189

Query: 199 GFMMDEGQASPEDEFRVFYADRSPWGLIINAKGTPGHGSRMYDNSAMENLMKSVEIVSRF 258
           GF MDEGQA+P DEFRVFYADR PW  +I A+G PGHG+++YDNSAMENLMKSVE++SRF
Sbjct: 190 GFAMDEGQANPGDEFRVFYADRVPWHFVIKAEGIPGHGAKLYDNSAMENLMKSVELISRF 249

Query: 259 RESQFDIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPD 318
           RESQFD VKAG+AA SEVISVNPV++ AG P+ TGFVMNMQPSEAEAG+DLR+PP ADPD
Sbjct: 250 RESQFDFVKAGKAAYSEVISVNPVYLKAGTPTTTGFVMNMQPSEAEAGYDLRLPPMADPD 309

Query: 319 SIRRRISEEWAPARRNMTFQIIQKGPIRDYLGRPLMTMTNDSNPWWSVFKTAISDAGGKL 378
            +++RI+EEWAP+ RNMT+ I +KG +RD+LGRP+MT  NDSNPWWS+FK A+   GGKL
Sbjct: 310 VMKKRIAEEWAPSIRNMTYSIQEKGKLRDHLGRPIMTPVNDSNPWWSIFKQAVEAMGGKL 369

Query: 379 SRPEILASTTDARFMRQLGIPVLGFSPMTNTPILLHDHNEFLKDTVFLRGIKVYESIISA 438
           ++PEILASTTD+RF+R LGIP  GFSPMTNTPILLHDHNEFLKDTVF++GI+VYES+ISA
Sbjct: 370 AKPEILASTTDSRFIRTLGIPTFGFSPMTNTPILLHDHNEFLKDTVFMKGIEVYESVISA 429

Query: 439 LSSFEEDASK 449
           LSSFE ++++
Sbjct: 430 LSSFEGESAQ 436

[Close]

BLAST of Lag0029250 vs. TAIR 10
Match: AT1G44180.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 636.3 bits (1640), Expect = 1.8e-182
Identity = 300/434 (69.12%), Postives = 366/434 (84.33%), Query Frame = 0


Query: 13  LATSLLLLS---FPLSFSHPQYGDDEADQDSPVSRFQHYLRIKTAHPNPDYASAVAFLKS 72
           +A SLLL +   F   FS   +  +E ++D+P++RFQ YLR  TAHPNP+Y + ++FL  
Sbjct: 1   MAVSLLLWTLFLFSFIFSLQSHSKEE-EEDTPITRFQQYLRFNTAHPNPNYTAPISFLLD 60

Query: 73  QALAIGLHAQILEFVPGKPLLLVTWYGSNPSLPSILLNSHMDSVPAEPSKWLHPPFSAVR 132
           QA +IGL ++ +EFVPGKP+LL+TW GSN +L SIL NSH+DSVPAE  KW+HPPFSA R
Sbjct: 61  QARSIGLTSRTIEFVPGKPVLLLTWLGSNLNLSSILFNSHLDSVPAESEKWIHPPFSAHR 120

Query: 133 TADGKIFARGSQDDKCIAVQYLEAIRNLRSRNFVPVRTIHISYVPDEEIGGFDGAAKFVL 192
           T DG I+ARG+QDDKCI VQYLEAIRNL+SR+F P+RTIHISYVP+EEIGGF G  KF  
Sbjct: 121 TIDGHIYARGAQDDKCIGVQYLEAIRNLKSRSFSPLRTIHISYVPEEEIGGFGGMMKFAA 180

Query: 193 SKEFKELNVGFMMDEGQASPEDEFRVFYADRSPWGLIINAKGTPGHGSRMYDNSAMENLM 252
           S EFK+LN+GF+MDEGQASP DEFRVFYA+R+PW L I A G PGHG+++YDNSAMENLM
Sbjct: 181 SSEFKDLNLGFVMDEGQASPGDEFRVFYAERTPWHLAIRANGMPGHGAKLYDNSAMENLM 240

Query: 253 KSVEIVSRFRESQFDIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDL 312
           KSVE++SRFRESQFD VKAG+AANSEVISVNPV++ AG PS TGFVMNMQPSEAE G+DL
Sbjct: 241 KSVELISRFRESQFDFVKAGKAANSEVISVNPVYLKAGTPSTTGFVMNMQPSEAEVGYDL 300

Query: 313 RIPPTADPDSIRRRISEEWAPARRNMTFQIIQKGPIRDYLGRPLMTMTNDSNPWWSVFKT 372
           R+PP ADP ++++RI+EEWAP+ RNMT+ + Q+G + D+LGRP+MT TND+NPWWS+FK 
Sbjct: 301 RLPPMADPVALKKRIAEEWAPSIRNMTYTLKQQGKLTDHLGRPIMTTTNDTNPWWSIFKQ 360

Query: 373 AISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMTNTPILLHDHNEFLKDTVFLRGI 432
           A+   GGKL++PEIL STTD+RF+R LGIPVLGFSPM NTPIL+HDHNEFLKDTVF++GI
Sbjct: 361 AVEATGGKLAKPEILISTTDSRFIRTLGIPVLGFSPMINTPILVHDHNEFLKDTVFMKGI 420

Query: 433 KVYESIISALSSFE 444
            VYES+ISALSSFE
Sbjct: 421 GVYESVISALSSFE 433

[Close]

BLAST of Lag0029250 vs. TAIR 10
Match: AT4G38220.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 503.8 bits (1296), Expect = 1.4e-142
Identity = 232/408 (56.86%), Postives = 304/408 (74.51%), Query Frame = 0


Query: 36  ADQDSPVSRFQHYLRIKTAHPNPDYASAVAFLKSQALAIGLHAQILEFVPGKPLLLVTWY 95
           A  D+ VSRFQ YLRI T  PNP+Y  AV F+ SQA  + L +Q +EFV GKPLLL+ W 
Sbjct: 19  AGDDAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLSLESQTIEFVKGKPLLLLKWV 78

Query: 96  GSNPSLPSILLNSHMDSVPAEPSKWLHPPFSAVRTADGKIFARGSQDDKCIAVQYLEAIR 155
           GS+P+LP+ LLNSH D VP E SKW H P  A     G I+ARGSQD KC+ +QYLEAIR
Sbjct: 79  GSDPTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLEAIR 138

Query: 156 NLRSRNFVPVRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPEDEFRV 215
            L++  F P+R++++S+VPDEEIGG DGA KF  S+ FK LN+  ++DEG  SP + +RV
Sbjct: 139 KLQASGFKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKSLNIAIVLDEGLPSPTESYRV 198

Query: 216 FYADRSPWGLIINAKGTPGHGSRMYDNSAMENLMKSVEIVSRFRESQFDIVKAGEAANSE 275
           FY +RSPW L+I AKG PGHG+++YDNSAMENL+KS+E + RFR SQFD++KAG  A  +
Sbjct: 199 FYGERSPWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESIRRFRASQFDLLKAGGIAEGD 258

Query: 276 VISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDSIRRRISEEWAPARRNM 335
           V+SVN  F+ AG PSPTGFVMN+QPSEAEAGFD+R+PP+ D +++ RR+ EEWAPA RNM
Sbjct: 259 VVSVNMAFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSVDAEALERRLVEEWAPAARNM 318

Query: 336 TFQIIQKGPIRDYLGRPLMTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQ 395
           +F+  QK       G+  +T  +DSNPWW + + A+ +AGG+ S+PEI  ++TDAR+ R+
Sbjct: 319 SFEFKQK-----LTGKQFLTAADDSNPWWGLLENAVKEAGGRTSKPEIFPASTDARYFRK 378

Query: 396 LGIPVLGFSPMTNTPILLHDHNEFLKDTVFLRGIKVYESIISALSSFE 444
            G+P  GFSP++NTP LLHDHNE+L    +L+GI+VY SII A +S+E
Sbjct: 379 AGVPAFGFSPISNTPSLLHDHNEYLGKAEYLKGIEVYVSIIKAYASYE 421

[Close]

BLAST of Lag0029250 vs. TAIR 10
Match: AT4G38220.2 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 502.7 bits (1293), Expect = 3.1e-142
Identity = 232/409 (56.72%), Postives = 306/409 (74.82%), Query Frame = 0


Query: 36  ADQDSPVSRFQHYLRIKTAHPNPDYASAVAFLKSQALAIGLHAQILEFVPGKPLLLVTWY 95
           A  D+ VSRFQ YLRI T  PNP+Y  AV F+ SQA  + L +Q +EFV GKPLLL+ W 
Sbjct: 19  AGDDAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLSLESQTIEFVKGKPLLLLKWV 78

Query: 96  GSNPSLPSILLNSHMDSVPAEPSKWLHPPFSAVRTADGKIFARGSQDDKCIAVQYLEAIR 155
           GS+P+LP+ LLNSH D VP E SKW H P  A     G I+ARGSQD KC+ +QYLEAIR
Sbjct: 79  GSDPTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLEAIR 138

Query: 156 NLRSRNFVPVRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPEDEFRV 215
            L++  F P+R++++S+VPDEEIGG DGA KF  S+ FK LN+  ++DEG  SP + +RV
Sbjct: 139 KLQASGFKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKSLNIAIVLDEGLPSPTESYRV 198

Query: 216 FYADRSPWGLIINAKGTPGHGSRMYDNSAMENLMKSVEIVSRFRESQFDIVKAGEAANSE 275
           FY +RSPW L+I AKG PGHG+++YDNSAMENL+KS+E + RFR SQFD++KAG  A  +
Sbjct: 199 FYGERSPWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESIRRFRASQFDLLKAGGIAEGD 258

Query: 276 VISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDSIRRRISEEWAPARRNM 335
           V+SVN  F+ AG PSPTGFVMN+QPSEAEAGFD+R+PP+ D +++ RR+ EEWAPA RNM
Sbjct: 259 VVSVNMAFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSVDAEALERRLVEEWAPAARNM 318

Query: 336 TFQIIQKGPIRDYL-GRPLMTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMR 395
           +F++   G  +  L G+  +T  +DSNPWW + + A+ +AGG+ S+PEI  ++TDAR+ R
Sbjct: 319 SFEL---GQFKQKLTGKQFLTAADDSNPWWGLLENAVKEAGGRTSKPEIFPASTDARYFR 378

Query: 396 QLGIPVLGFSPMTNTPILLHDHNEFLKDTVFLRGIKVYESIISALSSFE 444
           + G+P  GFSP++NTP LLHDHNE+L    +L+GI+VY SII A +S+E
Sbjct: 379 KAGVPAFGFSPISNTPSLLHDHNEYLGKAEYLKGIEVYVSIIKAYASYE 424

The following BLAST results are available for this feature:

BLAST of Lag0029250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879728.1	2.0e-236	89.93	aminoacylase-1-like [Benincasa hispida]	[more]
XP_022964785.1	3.8e-235	89.96	aminoacylase-1 [Cucurbita moschata]	[more]
KAG6602086.1	8.5e-235	90.38	Aminoacylase-1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7032791.1	8.5e-235	90.38	Aminoacylase-1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022991055.1	2.5e-234	89.51	aminoacylase-1 [Cucurbita maxima]	[more]

BLAST of Lag0029250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q99JW2	8.1e-95	43.41	Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1	[more]
P37111	6.9e-94	43.00	Aminoacylase-1 OS=Sus scrofa OX=9823 GN=ACY1 PE=1 SV=2	[more]
Q6AYS7	2.2e-92	43.14	Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1	[more]
Q6PTT0	4.2e-91	41.38	Aminoacylase-1B OS=Rattus norvegicus OX=10116 GN=Acy1b PE=1 SV=1	[more]
Q5RFB0	9.3e-91	41.87	Aminoacylase-1 OS=Pongo abelii OX=9601 GN=ACY1 PE=2 SV=2	[more]

BLAST of Lag0029250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HLT6	1.9e-235	89.96	aminoacylase-1 OS=Cucurbita moschata OX=3662 GN=LOC111464784 PE=4 SV=1	[more]
A0A6J1JRT3	1.2e-234	89.51	aminoacylase-1 OS=Cucurbita maxima OX=3661 GN=LOC111487760 PE=4 SV=1	[more]
A0A5D3BIQ8	1.8e-230	87.25	Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00191...	[more]
A0A5A7URV3	3.1e-230	86.80	Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G00182...	[more]
A0A6J1CK37	4.4e-229	89.51	aminoacylase-1 OS=Momordica charantia OX=3673 GN=LOC111011881 PE=4 SV=1	[more]

BLAST of Lag0029250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G44820.1	2.8e-183	68.60	Peptidase M20/M25/M40 family protein	[more]
AT1G44180.1	1.8e-182	69.12	Peptidase M20/M25/M40 family protein	[more]
AT4G38220.1	1.4e-142	56.86	Peptidase M20/M25/M40 family protein	[more]
AT4G38220.2	3.1e-142	56.72	Peptidase M20/M25/M40 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.150.900		coord: 361..442 e-value: 9.9E-29 score: 100.9
None	No IPR available	GENE3D	3.40.630.10	Zn peptidases	coord: 32..224 e-value: 2.0E-54 score: 186.1
None	No IPR available	GENE3D	3.30.70.360		coord: 225..334 e-value: 4.1E-9 score: 38.2
None	No IPR available	PIRSF	PIRSF036696	ACY-1	coord: 32..444 e-value: 2.5E-170 score: 564.3
None	No IPR available	PANTHER	PTHR45892	AMINOACYLASE-1	coord: 27..445
None	No IPR available	PANTHER	PTHR45892:SF3	PUTATIVE-RELATED	coord: 27..445
None	No IPR available	CDD	cd05646	M20_AcylaseI_like	coord: 39..440 e-value: 0.0 score: 639.319
None	No IPR available	SUPERFAMILY	53187	Zn-dependent exopeptidases	coord: 35..439
IPR002933	Peptidase M20	PFAM	PF01546	Peptidase_M20	coord: 105..437 e-value: 6.2E-34 score: 118.0
IPR011650	Peptidase M20, dimerisation domain	PFAM	PF07687	M20_dimer	coord: 222..326 e-value: 4.3E-7 score: 29.8
IPR010159	N-acyl-L-amino-acid amidohydrolase	TIGRFAM	TIGR01880	TIGR01880	coord: 36..442 e-value: 5.6E-182 score: 602.8
IPR036264	Bacterial exopeptidase dimerisation domain	SUPERFAMILY	55031	Bacterial exopeptidase dimerisation domain	coord: 225..330

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0029250.1	Lag0029250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006520	cellular amino acid metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004046	aminoacylase activity
molecular_function	GO:0016787	hydrolase activity