Lag0029228 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0029228
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	E3 ubiquitin-protein ligase RING1-like
Location	chr8: 36735304 .. 36735978 (+)
RNA-Seq Expression	Lag0029228
Synteny	Lag0029228

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATGGATACTCTCTACGACTACCGCATATGGCAACCTGAAGAACACGAAGAAGACGTTAATGTTACCATTTCCAATTCTCACTTGCTTCCCTGCGACTGTTTCCTTTTCCACCTTCACTTTCGACATGTTCTCCAGTCAAATCATCCTCAAGAATTGCAGAGGCTCCTGAATTCGTCTCTATCTTCGTTCTTGATACCTTGCGATGTCTTTTTCGCCACTGGCTCCAGTATCCTGATCGCCGTTTTCTCCGGCACCGGCGTCGCCCAAGACTTCTTGGACGCCGCGGTGCCGGACGTTCTGTCCGTCGCGGCCGATATAGCATGCAATCCTCTTAATGCTGGCCGTCGGATACTTCCGTTGGCGGTGTCCGTTTTAGTCGTTTCGCCTTACAATGAACGCGAGGAAATTGGGCCAGATTTGCGGGAGGCGCAAGCGTTTAAAACGTTGCCGGCGAGTGAGTCGGCAATCGAAGGGCTGAAGAACGTGGAGATTGATGTCGGCGGAGAGTTATTGATTGGTGACTGCAGAATATGTTTGGATGAACTGATGAATGGAGTGGAAGTTACGCAATTACCGTGCGCTCATTTTTACCACAGAGACTGCATCGTCAAATGGCTGGAGACGAGTCATTTGTGCCCGTTGTGTCGCTACGCAATGCCACTGAGCTGA

mRNA sequence

ATGGCGATGGATACTCTCTACGACTACCGCATATGGCAACCTGAAGAACACGAAGAAGACGTTAATGTTACCATTTCCAATTCTCACTTGCTTCCCTGCGACTGTTTCCTTTTCCACCTTCACTTTCGACATGTTCTCCAGTCAAATCATCCTCAAGAATTGCAGAGGCTCCTGAATTCGTCTCTATCTTCGTTCTTGATACCTTGCGATGTCTTTTTCGCCACTGGCTCCAGTATCCTGATCGCCGTTTTCTCCGGCACCGGCGTCGCCCAAGACTTCTTGGACGCCGCGGTGCCGGACGTTCTGTCCGTCGCGGCCGATATAGCATGCAATCCTCTTAATGCTGGCCGTCGGATACTTCCGTTGGCGGTGTCCGTTTTAGTCGTTTCGCCTTACAATGAACGCGAGGAAATTGGGCCAGATTTGCGGGAGGCGCAAGCGTTTAAAACGTTGCCGGCGAGTGAGTCGGCAATCGAAGGGCTGAAGAACGTGGAGATTGATGTCGGCGGAGAGTTATTGATTGGTGACTGCAGAATATGTTTGGATGAACTGATGAATGGAGTGGAAGTTACGCAATTACCGTGCGCTCATTTTTACCACAGAGACTGCATCGTCAAATGGCTGGAGACGAGTCATTTGTGCCCGTTGTGTCGCTACGCAATGCCACTGAGCTGA

Coding sequence (CDS)

ATGGCGATGGATACTCTCTACGACTACCGCATATGGCAACCTGAAGAACACGAAGAAGACGTTAATGTTACCATTTCCAATTCTCACTTGCTTCCCTGCGACTGTTTCCTTTTCCACCTTCACTTTCGACATGTTCTCCAGTCAAATCATCCTCAAGAATTGCAGAGGCTCCTGAATTCGTCTCTATCTTCGTTCTTGATACCTTGCGATGTCTTTTTCGCCACTGGCTCCAGTATCCTGATCGCCGTTTTCTCCGGCACCGGCGTCGCCCAAGACTTCTTGGACGCCGCGGTGCCGGACGTTCTGTCCGTCGCGGCCGATATAGCATGCAATCCTCTTAATGCTGGCCGTCGGATACTTCCGTTGGCGGTGTCCGTTTTAGTCGTTTCGCCTTACAATGAACGCGAGGAAATTGGGCCAGATTTGCGGGAGGCGCAAGCGTTTAAAACGTTGCCGGCGAGTGAGTCGGCAATCGAAGGGCTGAAGAACGTGGAGATTGATGTCGGCGGAGAGTTATTGATTGGTGACTGCAGAATATGTTTGGATGAACTGATGAATGGAGTGGAAGTTACGCAATTACCGTGCGCTCATTTTTACCACAGAGACTGCATCGTCAAATGGCTGGAGACGAGTCATTTGTGCCCGTTGTGTCGCTACGCAATGCCACTGAGCTGA

Protein sequence

MAMDTLYDYRIWQPEEHEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSLSSFLIPCDVFFATGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRILPLAVSVLVVSPYNEREEIGPDLREAQAFKTLPASESAIEGLKNVEIDVGGELLIGDCRICLDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS

Homology

[Close]

BLAST of Lag0029228 vs. NCBI nr
Match: XP_023554199.1 (probable E3 ubiquitin-protein ligase ATL45 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 425.2 bits (1092), Expect = 3.4e-115
Identity = 207/226 (91.59%), Postives = 216/226 (95.58%), Query Frame = 0


Query: 1   MAMDTLYDYRIWQPEEHEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60
           MAMDTLYDY+IWQPEEHE+D+NVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS
Sbjct: 1   MAMDTLYDYQIWQPEEHEDDINVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60

Query: 61  SLSSFLIPCDVFFATGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRIL 120
           SLSSFLIPCD FFA+G SILIAVFS TGVAQDFLDAAVPDVLSVAADIACNPLNAGRR+L
Sbjct: 61  SLSSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRVL 120

Query: 121 PLAVSVLVVSPYNEREEIGPDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCR 180
           PLAVSVL VSPYNEREEIG  LRE+  QAFKT PASESAIEGLK VEIDVGGELLIG+CR
Sbjct: 121 PLAVSVLAVSPYNEREEIGRVLRESSPQAFKTSPASESAIEGLKTVEIDVGGELLIGECR 180

Query: 181 ICLDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 225
           ICLDELMNG+EVT+LPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTRLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Lag0029228 vs. NCBI nr
Match: XP_022963379.1 (probable E3 ubiquitin-protein ligase ATL45 [Cucurbita moschata] >XP_022963389.1 probable E3 ubiquitin-protein ligase ATL45 [Cucurbita moschata])

HSP 1 Score: 423.7 bits (1088), Expect = 9.9e-115
Identity = 207/226 (91.59%), Postives = 215/226 (95.13%), Query Frame = 0


Query: 1   MAMDTLYDYRIWQPEEHEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60
           MAMDTLYDY+IWQPEEHE+DVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS
Sbjct: 1   MAMDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60

Query: 61  SLSSFLIPCDVFFATGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRIL 120
           SLSSFLIPCD FFA+G SILIAVFS TGVAQDFLDAAVPDVLSVAADIACNPLNAGRR+L
Sbjct: 61  SLSSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRVL 120

Query: 121 PLAVSVLVVSPYNEREEIGPDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCR 180
           PLAVSVL VSPYNEREEIG  LRE+  Q FKT PASESAIEGLK VEIDVGGELLIG+CR
Sbjct: 121 PLAVSVLAVSPYNEREEIGRVLRESSPQEFKTSPASESAIEGLKTVEIDVGGELLIGECR 180

Query: 181 ICLDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 225
           ICLDELMNG+EVT+LPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTRLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Lag0029228 vs. NCBI nr
Match: XP_022990569.1 (probable E3 ubiquitin-protein ligase ATL45 [Cucurbita maxima] >XP_022990570.1 probable E3 ubiquitin-protein ligase ATL45 [Cucurbita maxima])

HSP 1 Score: 423.3 bits (1087), Expect = 1.3e-114
Identity = 207/226 (91.59%), Postives = 215/226 (95.13%), Query Frame = 0


Query: 1   MAMDTLYDYRIWQPEEHEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60
           MAMDTLYDY+IWQPEEHE+DVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS
Sbjct: 1   MAMDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60

Query: 61  SLSSFLIPCDVFFATGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRIL 120
           SLSSFLIPCD FFA+G SILIAVFS TGVAQDFLDAAVPDVLSVAADIACNPLNAGRR+L
Sbjct: 61  SLSSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRVL 120

Query: 121 PLAVSVLVVSPYNEREEIGPDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCR 180
           PLAVSVL VSPYNEREEIG  LRE+  QAFKT PASESAIEGLK VEIDVGGELLIG+CR
Sbjct: 121 PLAVSVLAVSPYNEREEIGRVLRESSPQAFKTSPASESAIEGLKTVEIDVGGELLIGECR 180

Query: 181 ICLDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 225
           ICLDELMNG+EVT+LPCAH YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Lag0029228 vs. NCBI nr
Match: KAG6602099.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032803.1 E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 422.9 bits (1086), Expect = 1.7e-114
Identity = 207/226 (91.59%), Postives = 216/226 (95.58%), Query Frame = 0


Query: 1   MAMDTLYDYRIWQPEEHEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60
           MAMDTLYDY+IWQPEEHE+DVNVTISNSHLLPCDCFLFHLHFRHVLQSN+PQELQRLLNS
Sbjct: 1   MAMDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNNPQELQRLLNS 60

Query: 61  SLSSFLIPCDVFFATGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRIL 120
           SLSSFLIPCD FFA+G SILIAVFS TGVAQDFLDAAVPDVLSVAADIACNPLNAGRR+L
Sbjct: 61  SLSSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRVL 120

Query: 121 PLAVSVLVVSPYNEREEIGPDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCR 180
           PLAVSVL VSPYNEREEIG  LRE+  QAFKT PASESAIEGLK VEIDVGGELLIG+CR
Sbjct: 121 PLAVSVLAVSPYNEREEIGRVLRESSPQAFKTSPASESAIEGLKTVEIDVGGELLIGECR 180

Query: 181 ICLDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 225
           ICLDELMNG+EVT+LPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTRLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Lag0029228 vs. NCBI nr
Match: XP_038885922.1 (E3 ubiquitin-protein ligase RING1-like [Benincasa hispida])

HSP 1 Score: 410.6 bits (1054), Expect = 8.6e-111
Identity = 202/226 (89.38%), Postives = 211/226 (93.36%), Query Frame = 0


Query: 1   MAMDTLYDYRIWQPEEHEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60
           MAMDTLYDY IWQP+E E D+NVT+SNSHLLPCDCFLFHLHFRHVLQSNHPQE   LLNS
Sbjct: 1   MAMDTLYDYHIWQPQEGEGDLNVTMSNSHLLPCDCFLFHLHFRHVLQSNHPQEFHLLLNS 60

Query: 61  SLSSFLIPCDVFFATGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRIL 120
           SLSSFLIPC+VF ATGSSILIAVFS TGVAQDFLD AVPDVLSVAADIACNPLNAGRRIL
Sbjct: 61  SLSSFLIPCNVFLATGSSILIAVFSDTGVAQDFLDTAVPDVLSVAADIACNPLNAGRRIL 120

Query: 121 PLAVSVLVVSPYNEREEIGPDLRE--AQAFKTLPASESAIEGLKNVEIDVGGELLIGDCR 180
           PLAVSVLVVSPYNEREEIG  LRE  AQ FKTLPASE+AIEGLKNV+IDVGGELLIG+CR
Sbjct: 121 PLAVSVLVVSPYNEREEIGRVLRESSAQPFKTLPASETAIEGLKNVDIDVGGELLIGECR 180

Query: 181 ICLDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 225
           ICLDELMNG+EVT+LPCAH YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTRLPCAHVYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Lag0029228 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 6.0e-14
Identity = 40/113 (35.40%), Postives = 58/113 (51.33%), Query Frame = 0


Query: 111 NPLNAGRRILPLAVSVLVVSPYNEREEIGPDLREAQAFKTLPASESAIEGLKNVEIDVGG 170
           NP + G R LP  +    + P  E+        +   + T PAS+SAIE L  V I    
Sbjct: 158 NPSDQGFR-LPANIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSN 217

Query: 171 -ELLIGDCRICLDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMP 223
                  C +C+D+   G E  Q+PC H YH+DC++ WLE  + CP+CR+ +P
Sbjct: 218 LNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELP 269

[Close]

BLAST of Lag0029228 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 5.1e-13
Identity = 32/78 (41.03%), Postives = 52/78 (66.67%), Query Frame = 0


Query: 148 FKTLPASESAIEGLKNVEIDVGGELL---IGDCRICLDELMNGVEVTQLPCAHFYHRDCI 207
           + T PAS+SAI+ L  V+  V  ++L   +  C +C+DE  +G +V Q+PC H +H+DC+
Sbjct: 186 YGTPPASKSAIDALPTVK--VTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCL 245

Query: 208 VKWLETSHLCPLCRYAMP 223
           + WLE  + CP+CR+ +P
Sbjct: 246 LPWLELHNSCPVCRFELP 261

[Close]

BLAST of Lag0029228 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 9.5e-12
Identity = 30/71 (42.25%), Postives = 42/71 (59.15%), Query Frame = 0


Query: 152 PASESAIEGLKNVEIDVGGELLIGDCRICLDELMNGVEVTQLPCAHFYHRDCIVKWLETS 211
           PAS+SAIE L  VEI         +C +C +    G+E  ++PC H +H DCIV WL   
Sbjct: 190 PASKSAIESLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIR 249

Query: 212 HLCPLCRYAMP 223
           + CP+CR+ +P
Sbjct: 250 NSCPVCRFELP 260

[Close]

BLAST of Lag0029228 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 9.5e-12
Identity = 42/118 (35.59%), Postives = 62/118 (52.54%), Query Frame = 0


Query: 115 AGRRILPLAVSVLVVSPYNEREEIGPDLREAQAFKT-------LPASESAIEGLKNVEID 174
           +G R LP +VS +++    ER  +   L + +A  T        PAS+SAIE L  VEI 
Sbjct: 132 SGLRPLPDSVSEILMGSGFER--LLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEIS 191

Query: 175 ---VGGELLIGDCRICLDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMP 223
              +G E    +C +C +      E  ++PC H +H DCIV WL   + CP+CR+ +P
Sbjct: 192 DCHIGSE---ANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELP 244

[Close]

BLAST of Lag0029228 vs. ExPASy Swiss-Prot
Match: Q9SPL2 (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.6e-11
Identity = 29/70 (41.43%), Postives = 43/70 (61.43%), Query Frame = 0


Query: 152 PASESAIEGLKNVEIDVGGELLIGDCRICLDELMNGVEVTQLPCAHFYHRDCIVKWLETS 211
           PA++SAIE L+  E+      ++  C +C D ++ G    +LPC H YH DCIV WL T 
Sbjct: 232 PAAKSAIEALETFEVSSSEGEMVMVCAVCKDGMVMGETGKKLPCGHCYHGDCIVPWLGTR 291

Query: 212 HLCPLCRYAM 222
           + CP+CR+ +
Sbjct: 292 NSCPVCRFQL 301

[Close]

BLAST of Lag0029228 vs. ExPASy TrEMBL
Match: A0A6J1HJY0 (probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita moschata OX=3662 GN=LOC111463602 PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 4.8e-115
Identity = 207/226 (91.59%), Postives = 215/226 (95.13%), Query Frame = 0


Query: 1   MAMDTLYDYRIWQPEEHEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60
           MAMDTLYDY+IWQPEEHE+DVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS
Sbjct: 1   MAMDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60

Query: 61  SLSSFLIPCDVFFATGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRIL 120
           SLSSFLIPCD FFA+G SILIAVFS TGVAQDFLDAAVPDVLSVAADIACNPLNAGRR+L
Sbjct: 61  SLSSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRVL 120

Query: 121 PLAVSVLVVSPYNEREEIGPDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCR 180
           PLAVSVL VSPYNEREEIG  LRE+  Q FKT PASESAIEGLK VEIDVGGELLIG+CR
Sbjct: 121 PLAVSVLAVSPYNEREEIGRVLRESSPQEFKTSPASESAIEGLKTVEIDVGGELLIGECR 180

Query: 181 ICLDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 225
           ICLDELMNG+EVT+LPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTRLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Lag0029228 vs. ExPASy TrEMBL
Match: A0A6J1JSE1 (probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita maxima OX=3661 GN=LOC111487409 PE=4 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 6.2e-115
Identity = 207/226 (91.59%), Postives = 215/226 (95.13%), Query Frame = 0


Query: 1   MAMDTLYDYRIWQPEEHEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60
           MAMDTLYDY+IWQPEEHE+DVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS
Sbjct: 1   MAMDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60

Query: 61  SLSSFLIPCDVFFATGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRIL 120
           SLSSFLIPCD FFA+G SILIAVFS TGVAQDFLDAAVPDVLSVAADIACNPLNAGRR+L
Sbjct: 61  SLSSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRVL 120

Query: 121 PLAVSVLVVSPYNEREEIGPDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCR 180
           PLAVSVL VSPYNEREEIG  LRE+  QAFKT PASESAIEGLK VEIDVGGELLIG+CR
Sbjct: 121 PLAVSVLAVSPYNEREEIGRVLRESSPQAFKTSPASESAIEGLKTVEIDVGGELLIGECR 180

Query: 181 ICLDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 225
           ICLDELMNG+EVT+LPCAH YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Lag0029228 vs. ExPASy TrEMBL
Match: A0A0A0KDU3 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G385070 PE=4 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 2.1e-110
Identity = 200/226 (88.50%), Postives = 210/226 (92.92%), Query Frame = 0


Query: 1   MAMDTLYDYRIWQPEEHEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60
           MAMDTLYDY IWQPEE E+D+NVTISNSHLLPC+CFLFHLHFRH+LQSNH Q+   LLNS
Sbjct: 1   MAMDTLYDYHIWQPEEGEDDLNVTISNSHLLPCNCFLFHLHFRHILQSNHSQQFHYLLNS 60

Query: 61  SLSSFLIPCDVFFATGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRIL 120
           SLSSFLIPCDVFFAT SSILIAVFSGTGVAQDFLD AVPDVLSVAADIACNPLNAGR IL
Sbjct: 61  SLSSFLIPCDVFFATASSILIAVFSGTGVAQDFLDTAVPDVLSVAADIACNPLNAGRTIL 120

Query: 121 PLAVSVLVVSPYNEREEIGPDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCR 180
           PLAVSVLVVSPYNER+EIG  LRE+  QAFKTLPASE AIEGLK VEIDVGGELLIG+CR
Sbjct: 121 PLAVSVLVVSPYNERQEIGRVLRESSPQAFKTLPASEMAIEGLKKVEIDVGGELLIGECR 180

Query: 181 ICLDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 225
           ICLDELMNG+EVT+LPCAH YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Lag0029228 vs. ExPASy TrEMBL
Match: A0A5A7V2F0 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold184G001370 PE=4 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 5.7e-108
Identity = 195/226 (86.28%), Postives = 208/226 (92.04%), Query Frame = 0


Query: 1   MAMDTLYDYRIWQPEEHEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60
           MAMDTLYDY IWQPEE E+D+NVTISNSHLLPC+CFLFHLHFRH+LQSNH Q+   LLNS
Sbjct: 1   MAMDTLYDYHIWQPEEGEDDLNVTISNSHLLPCNCFLFHLHFRHILQSNHSQQSHYLLNS 60

Query: 61  SLSSFLIPCDVFFATGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRIL 120
           SLSS+LIPCDVFFAT SSILIAVFSGTGVAQDFLD AVPDVLSVAADIACNPLNAGR IL
Sbjct: 61  SLSSYLIPCDVFFATASSILIAVFSGTGVAQDFLDTAVPDVLSVAADIACNPLNAGRTIL 120

Query: 121 PLAVSVLVVSPYNEREEIGPDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCR 180
           PL VSVLVVSPYNER+EIG  LRE+  QAFKTLPASE AIE LKNVEI VGGELLIG+CR
Sbjct: 121 PLTVSVLVVSPYNERQEIGQVLRESSPQAFKTLPASEMAIEDLKNVEIGVGGELLIGECR 180

Query: 181 ICLDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 225
           ICLDEL+NG+E+T+LPCAH YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELVNGMEITRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Lag0029228 vs. ExPASy TrEMBL
Match: A0A1S3CS28 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103504063 PE=4 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 5.7e-108
Identity = 195/226 (86.28%), Postives = 208/226 (92.04%), Query Frame = 0


Query: 1   MAMDTLYDYRIWQPEEHEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60
           MAMDTLYDY IWQPEE E+D+NVTISNSHLLPC+CFLFHLHFRH+LQSNH Q+   LLNS
Sbjct: 1   MAMDTLYDYHIWQPEEGEDDLNVTISNSHLLPCNCFLFHLHFRHILQSNHSQQSHYLLNS 60

Query: 61  SLSSFLIPCDVFFATGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRIL 120
           SLSS+LIPCDVFFAT SSILIAVFSGTGVAQDFLD AVPDVLSVAADIACNPLNAGR IL
Sbjct: 61  SLSSYLIPCDVFFATASSILIAVFSGTGVAQDFLDTAVPDVLSVAADIACNPLNAGRTIL 120

Query: 121 PLAVSVLVVSPYNEREEIGPDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCR 180
           PL VSVLVVSPYNER+EIG  LRE+  QAFKTLPASE AIE LKNVEI VGGELLIG+CR
Sbjct: 121 PLTVSVLVVSPYNERQEIGQVLRESSPQAFKTLPASEMAIEDLKNVEIGVGGELLIGECR 180

Query: 181 ICLDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 225
           ICLDEL+NG+E+T+LPCAH YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELVNGMEITRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Lag0029228 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 3.6e-14
Identity = 32/78 (41.03%), Postives = 52/78 (66.67%), Query Frame = 0


Query: 148 FKTLPASESAIEGLKNVEIDVGGELL---IGDCRICLDELMNGVEVTQLPCAHFYHRDCI 207
           + T PAS+SAI+ L  V+  V  ++L   +  C +C+DE  +G +V Q+PC H +H+DC+
Sbjct: 186 YGTPPASKSAIDALPTVK--VTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCL 245

Query: 208 VKWLETSHLCPLCRYAMP 223
           + WLE  + CP+CR+ +P
Sbjct: 246 LPWLELHNSCPVCRFELP 261

[Close]

BLAST of Lag0029228 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.8 bits (177), Expect = 4.0e-13
Identity = 33/75 (44.00%), Postives = 47/75 (62.67%), Query Frame = 0


Query: 152 PASESAIEGLKNVEIDVGGELLIGD---CRICLDELMNGVEVTQLPCAHFYHRDCIVKWL 211
           PASE  I  L +V+I    + L  D   C +C++E + G + T+LPC H YH+DCIV WL
Sbjct: 198 PASEPTINSLPSVKIT--PQHLTNDMSQCTVCMEEFIVGGDATELPCKHIYHKDCIVPWL 257

Query: 212 ETSHLCPLCRYAMPL 224
             ++ CP+CR  +PL
Sbjct: 258 RLNNSCPICRRDLPL 270

[Close]

BLAST of Lag0029228 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 5.2e-13
Identity = 31/75 (41.33%), Postives = 47/75 (62.67%), Query Frame = 0


Query: 148 FKTLPASESAIEGLKNVEIDVGGELLIGDCRICLDELMNGVEVTQLPCAHFYHRDCIVKW 207
           + T PA++ A+E L  V+I+    LL   C +CLD+   G+E  ++PC H +H DC++ W
Sbjct: 200 YGTPPATKEAVEALAMVKIE--DSLL--QCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPW 259

Query: 208 LETSHLCPLCRYAMP 223
           LE    CP+CRY +P
Sbjct: 260 LELHSSCPVCRYLLP 270

[Close]

BLAST of Lag0029228 vs. TAIR 10
Match: AT3G13430.2 (RING/U-box superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 5.2e-13
Identity = 31/75 (41.33%), Postives = 47/75 (62.67%), Query Frame = 0


Query: 148 FKTLPASESAIEGLKNVEIDVGGELLIGDCRICLDELMNGVEVTQLPCAHFYHRDCIVKW 207
           + T PA++ A+E L  V+I+    LL   C +CLD+   G+E  ++PC H +H DC++ W
Sbjct: 200 YGTPPATKEAVEALAMVKIE--DSLL--QCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPW 259

Query: 208 LETSHLCPLCRYAMP 223
           LE    CP+CRY +P
Sbjct: 260 LELHSSCPVCRYLLP 270

[Close]

BLAST of Lag0029228 vs. TAIR 10
Match: AT3G13430.3 (RING/U-box superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 5.2e-13
Identity = 31/75 (41.33%), Postives = 47/75 (62.67%), Query Frame = 0


Query: 148 FKTLPASESAIEGLKNVEIDVGGELLIGDCRICLDELMNGVEVTQLPCAHFYHRDCIVKW 207
           + T PA++ A+E L  V+I+    LL   C +CLD+   G+E  ++PC H +H DC++ W
Sbjct: 200 YGTPPATKEAVEALAMVKIE--DSLL--QCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPW 259

Query: 208 LETSHLCPLCRYAMP 223
           LE    CP+CRY +P
Sbjct: 260 LELHSSCPVCRYLLP 270

The following BLAST results are available for this feature:

BLAST of Lag0029228 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023554199.1	3.4e-115	91.59	probable E3 ubiquitin-protein ligase ATL45 [Cucurbita pepo subsp. pepo]	[more]
XP_022963379.1	9.9e-115	91.59	probable E3 ubiquitin-protein ligase ATL45 [Cucurbita moschata] >XP_022963389.1 ...	[more]
XP_022990569.1	1.3e-114	91.59	probable E3 ubiquitin-protein ligase ATL45 [Cucurbita maxima] >XP_022990570.1 pr...	[more]
KAG6602099.1	1.7e-114	91.59	E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038885922.1	8.6e-111	89.38	E3 ubiquitin-protein ligase RING1-like [Benincasa hispida]	[more]

BLAST of Lag0029228 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0CH30	6.0e-14	35.40	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q8LPN7	5.1e-13	41.03	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q9SNB6	9.5e-12	42.25	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
Q940T5	9.5e-12	35.59	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
Q9SPL2	3.6e-11	41.43	E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV...	[more]

BLAST of Lag0029228 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HJY0	4.8e-115	91.59	probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1JSE1	6.2e-115	91.59	probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A0A0KDU3	2.1e-110	88.50	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G385070 P...	[more]
A0A5A7V2F0	5.7e-108	86.28	E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CS28	5.7e-108	86.28	E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103504063 P...	[more]

BLAST of Lag0029228 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19950.1	3.6e-14	41.03	RING/U-box superfamily protein	[more]
AT1G60360.1	4.0e-13	44.00	RING/U-box superfamily protein	[more]
AT3G13430.1	5.2e-13	41.33	RING/U-box superfamily protein	[more]
AT3G13430.2	5.2e-13	41.33	RING/U-box superfamily protein	[more]
AT3G13430.3	5.2e-13	41.33	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 176..218 e-value: 0.0018 score: 13.5
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 177..217 e-value: 5.4E-7 score: 39.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 176..218 e-value: 2.1E-13 score: 50.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 177..218 score: 12.858103
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 141..223 e-value: 5.3E-19 score: 69.7
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 143..222
None	No IPR available	PANTHER	PTHR15710:SF120	F6A14.12 PROTEIN-RELATED	coord: 143..222
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 176..218 e-value: 3.00181E-15 score: 65.4009
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 176..222

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0029228.1	Lag0029228.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding