Lag0028994 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0028994
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	B3 domain-containing protein At2g32645-like
Location	chr8: 33925652 .. 33926395 (+)
RNA-Seq Expression	Lag0028994
Synteny	Lag0028994

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAATGGGGATGAACGATGAAGGCCTTCTGCTAGGGCTTCCTTTTCCAAATTCAATCATTGGAGTCCACATTCCTCGAAGACGAAGGTCTGGCAGTTCCGAATCGAGGCCCAAACACGAAGCTTGCATTTCCATGTTCGAGGCCGCGAGGATTCTGATCGCCATGGCCAACGGAGGCTTCTGTAAAGGTGAAATCGAAACGAATAATGGAAAGAGGAAGAAATCTCCAACGACGACGAAGAAACAGAGGAAGAAGAGGCGAAAGGAAATCAAGTTACCTAACAGTCCCGAGATGCCGGCGGCGATGAGAGATCGGATTGTGGAGATGGGCGGGTACGGAATCGCATTGGTGATTCAGAAGACACTTCAAGACACCGATATGAACAAAAACCACGGCCGATTGTCTCTTCCGCCGAAAAAGGTGAAAATCGATTTCGTGACGGAAGAAGAGAGGAAGTTGTTGAATGAACAAAATCAGAAGAACAAGAAGGGAATGAAGGTGATGATTATGGATGCTGTTCTTCGAGAAAGCTGGATATGCTTGAAGAAGTGGAAGATCGGGAGTGATCTAGTGTACTGTTTGATGACGAACTGGAATTCATTTGTGGAACAAAGCAATCTGAGAAGTGGAGATTGCATTCAACTTTGGAGCTTCAGGAAAGACGGCGTTGATCCTCAAACTGGAACAATGGCTTCTCATCAGCTATGTTTCGCCATGGTTAAACTTGACCTATCTGAATGA

mRNA sequence

ATGGGAATGGGGATGAACGATGAAGGCCTTCTGCTAGGGCTTCCTTTTCCAAATTCAATCATTGGAGTCCACATTCCTCGAAGACGAAGGTCTGGCAGTTCCGAATCGAGGCCCAAACACGAAGCTTGCATTTCCATGTTCGAGGCCGCGAGGATTCTGATCGCCATGGCCAACGGAGGCTTCTGTAAAGGTGAAATCGAAACGAATAATGGAAAGAGGAAGAAATCTCCAACGACGACGAAGAAACAGAGGAAGAAGAGGCGAAAGGAAATCAAGTTACCTAACAGTCCCGAGATGCCGGCGGCGATGAGAGATCGGATTGTGGAGATGGGCGGGTACGGAATCGCATTGGTGATTCAGAAGACACTTCAAGACACCGATATGAACAAAAACCACGGCCGATTGTCTCTTCCGCCGAAAAAGGTGAAAATCGATTTCGTGACGGAAGAAGAGAGGAAGTTGTTGAATGAACAAAATCAGAAGAACAAGAAGGGAATGAAGGTGATGATTATGGATGCTGTTCTTCGAGAAAGCTGGATATGCTTGAAGAAGTGGAAGATCGGGAGTGATCTAGTGTACTGTTTGATGACGAACTGGAATTCATTTGTGGAACAAAGCAATCTGAGAAGTGGAGATTGCATTCAACTTTGGAGCTTCAGGAAAGACGGCGTTGATCCTCAAACTGGAACAATGGCTTCTCATCAGCTATGTTTCGCCATGGTTAAACTTGACCTATCTGAATGA

Coding sequence (CDS)

ATGGGAATGGGGATGAACGATGAAGGCCTTCTGCTAGGGCTTCCTTTTCCAAATTCAATCATTGGAGTCCACATTCCTCGAAGACGAAGGTCTGGCAGTTCCGAATCGAGGCCCAAACACGAAGCTTGCATTTCCATGTTCGAGGCCGCGAGGATTCTGATCGCCATGGCCAACGGAGGCTTCTGTAAAGGTGAAATCGAAACGAATAATGGAAAGAGGAAGAAATCTCCAACGACGACGAAGAAACAGAGGAAGAAGAGGCGAAAGGAAATCAAGTTACCTAACAGTCCCGAGATGCCGGCGGCGATGAGAGATCGGATTGTGGAGATGGGCGGGTACGGAATCGCATTGGTGATTCAGAAGACACTTCAAGACACCGATATGAACAAAAACCACGGCCGATTGTCTCTTCCGCCGAAAAAGGTGAAAATCGATTTCGTGACGGAAGAAGAGAGGAAGTTGTTGAATGAACAAAATCAGAAGAACAAGAAGGGAATGAAGGTGATGATTATGGATGCTGTTCTTCGAGAAAGCTGGATATGCTTGAAGAAGTGGAAGATCGGGAGTGATCTAGTGTACTGTTTGATGACGAACTGGAATTCATTTGTGGAACAAAGCAATCTGAGAAGTGGAGATTGCATTCAACTTTGGAGCTTCAGGAAAGACGGCGTTGATCCTCAAACTGGAACAATGGCTTCTCATCAGCTATGTTTCGCCATGGTTAAACTTGACCTATCTGAATGA

Protein sequence

MGMGMNDEGLLLGLPFPNSIIGVHIPRRRRSGSSESRPKHEACISMFEAARILIAMANGGFCKGEIETNNGKRKKSPTTTKKQRKKRRKEIKLPNSPEMPAAMRDRIVEMGGYGIALVIQKTLQDTDMNKNHGRLSLPPKKVKIDFVTEEERKLLNEQNQKNKKGMKVMIMDAVLRESWICLKKWKIGSDLVYCLMTNWNSFVEQSNLRSGDCIQLWSFRKDGVDPQTGTMASHQLCFAMVKLDLSE

Homology

[Close]

BLAST of Lag0028994 vs. NCBI nr
Match: XP_022146365.1 (putative B3 domain-containing protein At2g27410 [Momordica charantia])

HSP 1 Score: 263.5 bits (672), Expect = 1.9e-66
Identity = 156/272 (57.35%), Postives = 192/272 (70.59%), Query Frame = 0


Query: 1   MGMG--MNDEGLLLGLPFPNSIIGVHIPRRRRSGSSES--------RPKHE----ACISM 60
           MGMG  +  E L +G+ F +   G+ IP+RRR+GSS S        +PK      A +SM
Sbjct: 1   MGMGTVVELEALKMGICFSDLTNGISIPQRRRTGSSSSSAETSETNKPKPSSSATAPMSM 60

Query: 61  FEAARILIAMANG-----GFCKGEIETNNGKRKKSPTTTK----KQRKKRRKEIKLPNSP 120
            EAA +L+AMA        FCK E+E N+ KRK+ P +      KQRKK+RK ++LP +P
Sbjct: 61  VEAAAVLLAMAYSATDVEAFCKSELEKNSRKRKRDPVSKSEPEMKQRKKKRKAMELPVTP 120

Query: 121 EMPAAMRDRIVEMGGYGIALVIQKTLQDTDMNKNHGRLSLPPKKVKI-DFVTEEERKLLN 180
           EMPAAMRDRIVE+GGYGI LVIQK LQ+TDMNKNHGRLSLP KK++I DFVTEEERKLLN
Sbjct: 121 EMPAAMRDRIVEIGGYGIQLVIQKQLQNTDMNKNHGRLSLPAKKLRIHDFVTEEERKLLN 180

Query: 181 E-QNQKNKKGMKVMIMDAVLRESWICLKKWKIGSDLVYCLMTNWNSFVEQSNLRSGDCIQ 240
           +  N  NKKGM V I+D  L+E  ICLKKWKIGS  +YCLMT WNSFVE++ LRSGD IQ
Sbjct: 181 QHSNNNNKKGMDVPIIDPDLKERRICLKKWKIGSGDLYCLMTQWNSFVEETALRSGDFIQ 240

Query: 241 LWSFRKDGVD--PQTGTMASHQLCFAMVKLDL 246
           LWSFRKD  D   +T  +++ +L  AMVKL++
Sbjct: 241 LWSFRKDEFDEAEETEKISTSRLWLAMVKLEV 272

[Close]

BLAST of Lag0028994 vs. NCBI nr
Match: XP_022990807.1 (B3 domain-containing protein At2g32645-like [Cucurbita maxima] >XP_022990957.1 B3 domain-containing protein At2g32645-like [Cucurbita maxima])

HSP 1 Score: 245.7 bits (626), Expect = 4.1e-61
Identity = 135/229 (58.95%), Postives = 157/229 (68.56%), Query Frame = 0


Query: 21  IGVHIPRRRRSGSSESRPKHEACISMFEAARILIAMANG-----GFCKGEIETNNGKRKK 80
           +GVHIPRR+R  S  +  KH   ISM E A ILIA +N            + T N K   
Sbjct: 17  LGVHIPRRKRGSSGSAITKHS--ISMMECATILIAFSNSVTHVLPSAYLPLRTTNTK--- 76

Query: 81  SPTTTKKQRKKRRKEIKLPNSPEMPAAMRDRIVEMGGYGIALVIQKTLQDTDMNKNHGRL 140
            P T +K RKKR K+   P +P+MPAAMRDRI EMG Y I L IQK LQDTDMNKNHGRL
Sbjct: 77  -PITERKPRKKRCKQTPFPTTPDMPAAMRDRIGEMGAYQIQLAIQKQLQDTDMNKNHGRL 136

Query: 141 SLPPKKVKIDFVTEEERKLLNEQNQKNKKGMKVMIMDAVLRESWICLKKWKIGSDLVYCL 200
           S P KK+K+DF TEEER++L +Q    KKGM VMI+D +LRES ICLK WKIGS   YCL
Sbjct: 137 SFPAKKLKVDFATEEERRVLKQQENGGKKGMNVMIVDPLLRESSICLKLWKIGSGDSYCL 196

Query: 201 MTNWNSFVEQSNLRSGDCIQLWSFRKDGVDPQTGTMASHQLCFAMVKLD 245
           MT WNS VEQ+  +SGD +QLWSFRKD  + +T TM S +LCFAMVKLD
Sbjct: 197 MTQWNSLVEQNGFKSGDHVQLWSFRKDEFEAETQTMVS-RLCFAMVKLD 238

[Close]

BLAST of Lag0028994 vs. NCBI nr
Match: XP_022959448.1 (putative B3 domain-containing protein At3g24850 [Cucurbita moschata])

HSP 1 Score: 244.6 bits (623), Expect = 9.0e-61
Identity = 136/243 (55.97%), Postives = 161/243 (66.26%), Query Frame = 0


Query: 21  IGVHIPRRRRSGSSESRPKHEACISMFEAARILIAMANG-----GFCKGEIETNNGK--- 80
           +GVHIPRR+R  S  +  K    ISM E A ILIA +N            + T NGK   
Sbjct: 17  LGVHIPRRKRGSSGSAITKRS--ISMMECATILIAFSNSVTHVLPSAYLPLRTTNGKPIT 76

Query: 81  -----------RKKSPTTTKKQRKKRRKEIKLPNSPEMPAAMRDRIVEMGGYGIALVIQK 140
                       ++ P T +K RKKR K+   P +P+MPAAMRDRI EMG Y I L IQK
Sbjct: 77  NTKPITKRKPITERKPITERKPRKKRCKQTPFPTTPDMPAAMRDRIGEMGAYQIQLAIQK 136

Query: 141 TLQDTDMNKNHGRLSLPPKKVKIDFVTEEERKLLNEQNQKNKKGMKVMIMDAVLRESWIC 200
            LQDTDMNKNHGRLS P KK+K+DF TEEER++L +Q  K KKGM VMI+D +LRES IC
Sbjct: 137 QLQDTDMNKNHGRLSFPAKKLKVDFATEEERRVLKQQENKGKKGMNVMIVDPLLRESSIC 196

Query: 201 LKKWKIGSDLVYCLMTNWNSFVEQSNLRSGDCIQLWSFRKDGVDPQTGTMASHQLCFAMV 245
           LK WKIGS   YCLMT WNS VEQ+  +SGD +QLWSFRKD ++ +T TM S +LCFAMV
Sbjct: 197 LKLWKIGSGDSYCLMTQWNSLVEQNGFKSGDHVQLWSFRKDELEAETQTMVS-RLCFAMV 256

[Close]

BLAST of Lag0028994 vs. NCBI nr
Match: XP_038875653.1 (B3 domain-containing protein At1g05920-like [Benincasa hispida])

HSP 1 Score: 244.2 bits (622), Expect = 1.2e-60
Identity = 145/250 (58.00%), Postives = 171/250 (68.40%), Query Frame = 0


Query: 9   GLLLGLPFPNSIIGVHIPRRRRSGSSESRPKHEA-----CISMFEAARILIAMANG---- 68
           G+ LGLP  N +    IPRRR S ++  + +H A      ISM EAA +L+A+AN     
Sbjct: 2   GMELGLPDLNKLC---IPRRRSSSAAGKKEQHLAESSKPPISMLEAAVVLMAIANSVSDV 61

Query: 69  -GFCKGEIE--TNNGKRKKSPTTT---KKQRKKRRKEIKLPNSPEMPAAMRDRIVEMGGY 128
             FC  E++   N  KRK  PTT    K  RKKR ++ K P+ PEMPAAMRDRIVEMGGY
Sbjct: 62  ESFCNCELKKYENTHKRKNPPTTAATKKPPRKKRARQSKFPSIPEMPAAMRDRIVEMGGY 121

Query: 129 GIALVIQKTLQDTDMNKNHGRLSLPPKKVKIDFVTEEERKLLNEQNQKNKKGMKVMIMDA 188
            I LVIQK LQDTD+NKN GRLSLP KK+  DF TEEER+LL++Q  KNKKG+ V+IMD 
Sbjct: 122 EINLVIQKQLQDTDLNKNLGRLSLPQKKLLFDFATEEERRLLSQQENKNKKGLNVVIMDH 181

Query: 189 VLRESWICLKKWKIGSDLVYCLMTNWNSFVEQSNLRSGDCIQLWSFRKDGVDPQTGTMAS 244
           VL E  ICLKKWKIGS  VYCLMT WN+FV++  L+SGD IQLWSFRKD  D   G    
Sbjct: 182 VLEERNICLKKWKIGSGDVYCLMTQWNAFVKERGLKSGDHIQLWSFRKD--DENEG---G 241

[Close]

BLAST of Lag0028994 vs. NCBI nr
Match: XP_023540870.1 (putative B3 domain-containing protein At3g24850 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 242.7 bits (618), Expect = 3.4e-60
Identity = 136/243 (55.97%), Postives = 159/243 (65.43%), Query Frame = 0


Query: 21  IGVHIPRRRRSGSSESRPKHEACISMFEAARILIAMANG-----GFCKGEIETNNGK--- 80
           +GVHIPRR+R  S  +  K    ISM E A ILIA +N            + T N K   
Sbjct: 17  LGVHIPRRKRGSSGSAITKRS--ISMMECATILIAFSNSVTHVLPSAYLPVRTTNTKPIT 76

Query: 81  -----------RKKSPTTTKKQRKKRRKEIKLPNSPEMPAAMRDRIVEMGGYGIALVIQK 140
                       K+ P T +K RKKR K+   P +P+MPAAMRDRI EMG Y I L IQK
Sbjct: 77  DRKPISERKPITKRKPITERKPRKKRCKQTPFPTTPDMPAAMRDRIGEMGAYQIELAIQK 136

Query: 141 TLQDTDMNKNHGRLSLPPKKVKIDFVTEEERKLLNEQNQKNKKGMKVMIMDAVLRESWIC 200
            LQDTDMNKNHGRLS P KK+K+DF TEEER++L +Q  K KKGM VMI+D +LRES IC
Sbjct: 137 QLQDTDMNKNHGRLSFPAKKLKVDFATEEERRVLKQQENKGKKGMNVMIVDPLLRESSIC 196

Query: 201 LKKWKIGSDLVYCLMTNWNSFVEQSNLRSGDCIQLWSFRKDGVDPQTGTMASHQLCFAMV 245
           LK WKIGS   YCLMT WNS VEQ+  +SGD +QLWSFRKD  + +T TM S +LCFAMV
Sbjct: 197 LKLWKIGSGDSYCLMTQWNSLVEQNGFKSGDHVQLWSFRKDEFEAETQTMVS-RLCFAMV 256

[Close]

BLAST of Lag0028994 vs. ExPASy Swiss-Prot
Match: Q9SIC7 (B3 domain-containing protein At2g31420 OS=Arabidopsis thaliana OX=3702 GN=At2g31420 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 3.6e-12
Identity = 48/132 (36.36%), Postives = 74/132 (56.06%), Query Frame = 0


Query: 112 GYGIALVI-QKTLQDTDMNKNHGRLSLPPKKV-KIDFVTEEERKLLNEQNQKNKK-GMKV 171
           GY   L+I ++ L  TD+ KN G LS+   K+ K DF+TE+E + L+E   K K+ G+KV
Sbjct: 73  GYDPKLIIKRRVLCTTDLRKNQGCLSMHLSKLEKSDFLTEDETRFLDEDFLKAKRDGLKV 132

Query: 172 MIMDAVLRESWICLKKWKIGSDLVYCLMTNWNSFVEQSNLRSGDCIQLWSFRKDGVDPQT 231
            ++D    +  + LKKW   +   Y L   WN+ +++   +  D I++WSFR DG     
Sbjct: 133 FLVDPESDKHVVYLKKWNGNTVWKYVLSHGWNNVIDKKIFKVNDVIEIWSFR-DG----- 192

Query: 232 GTMASHQLCFAM 241
               S +LCFA+
Sbjct: 193 ----SGKLCFAL 194

[Close]

BLAST of Lag0028994 vs. ExPASy Swiss-Prot
Match: Q9FLV7 (B3 domain-containing protein At5g24050 OS=Arabidopsis thaliana OX=3702 GN=At5g24050 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 4.7e-12
Identity = 46/131 (35.11%), Postives = 70/131 (53.44%), Query Frame = 0


Query: 117 LVIQKTLQDTDMNKNHGRLSLP-PKKVKIDFVTEEERKLLNEQ-NQKNKKGMKVMIMDAV 176
           L+ +KTL  TD+N    RLS+P    ++ DF+T  E  ++NE  N  NK G+  +++D  
Sbjct: 218 LIFEKTLFVTDVNPTKNRLSMPFNNLLRNDFLTSVESIIINEDINNNNKIGVGAILVDQR 277

Query: 177 LRESWICLKKWKIGSD-----LVYCLMTNWNSFVEQSNLRSGDCIQLWSFRKDGVDPQTG 236
            ++  + LK+W++  +       Y L+  WN  VE + L+ GD I LWSFR  G+     
Sbjct: 278 CKKWGVMLKRWEMKKESGKGSWSYNLICGWNDIVEANRLKEGDNITLWSFRCCGI----- 337

Query: 237 TMASHQLCFAM 241
                 LCFAM
Sbjct: 338 ------LCFAM 337

[Close]

BLAST of Lag0028994 vs. ExPASy Swiss-Prot
Match: Q9LSF7 (B3 domain-containing protein At3g25182 OS=Arabidopsis thaliana OX=3702 GN=At3g25182 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.8e-11
Identity = 43/131 (32.82%), Postives = 72/131 (54.96%), Query Frame = 0


Query: 117 LVIQKTLQDTDMNKNHGRLSLP-PKKVKIDFVTEEERKLLNEQNQKNKK-GMKVMIMDAV 176
           L+ +KTL  TD+N    RLS+P    ++ DF+T  E +++++  + +KK G+  +++D  
Sbjct: 215 LIFEKTLFVTDVNPTQNRLSMPFNNLLRNDFLTSVESRIIDKDIKNDKKIGVGAILVDQR 274

Query: 177 LRESWICLKKWKIGSD-----LVYCLMTNWNSFVEQSNLRSGDCIQLWSFRKDGVDPQTG 236
            ++  + LK+W++  +       Y L+  WN  VE + L+ GD I LWSFR  G+     
Sbjct: 275 CKKWGVMLKRWEMKKESGKGSWNYNLICGWNDIVEANGLKEGDNISLWSFRSCGI----- 334

Query: 237 TMASHQLCFAM 241
                 LCFAM
Sbjct: 335 ------LCFAM 334

[Close]

BLAST of Lag0028994 vs. ExPASy Swiss-Prot
Match: Q9LRX6 (Putative B3 domain-containing protein At3g24850 OS=Arabidopsis thaliana OX=3702 GN=At3g24850 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 5.2e-11
Identity = 45/131 (34.35%), Postives = 69/131 (52.67%), Query Frame = 0


Query: 117 LVIQKTLQDTDMNKNHGRLSLP-PKKVKIDFVTEEERKLLNEQNQKNKK-GMKVMIMDAV 176
           L+ +KTL  TD+N    RLS+P    ++ DF+T  E +++ E    NKK G+  +++D  
Sbjct: 228 LIFEKTLFVTDINPTQNRLSMPFNNLLQNDFLTSVESRIIKEDINNNKKIGVGAILVDQR 287

Query: 177 LRESWICLKKWKIGSD-----LVYCLMTNWNSFVEQSNLRSGDCIQLWSFRKDGVDPQTG 236
             +  + LK+W++  +       Y L+  WN  VE + L+ GD I +WSFR  GV     
Sbjct: 288 SVKWGVMLKRWELKKESGKGSWNYNLICGWNDVVEANGLKEGDNISVWSFRCRGV----- 347

Query: 237 TMASHQLCFAM 241
                 LCFAM
Sbjct: 348 ------LCFAM 347

[Close]

BLAST of Lag0028994 vs. ExPASy Swiss-Prot
Match: Q9MA32 (B3 domain-containing protein At1g05930 OS=Arabidopsis thaliana OX=3702 GN=At1g05930 PE=2 SV=2)

HSP 1 Score: 68.9 bits (167), Expect = 8.9e-11
Identity = 48/146 (32.88%), Postives = 77/146 (52.74%), Query Frame = 0


Query: 98  EMPAAMRDRIVEMGGYGIALVIQKTLQDTD-MNKNHGRLSLP-PKKVKIDFVTEEERKLL 157
           EM   +R  + +M    + L+++KTL  TD +   HGR S+   + +  DF+  EER +L
Sbjct: 159 EMRWWLRQVMKDMRAEDLTLILEKTLSTTDVITTTHGRFSMHFNRLISNDFLKPEERSIL 218

Query: 158 NEQNQKNK-KGMKVMIMDAVLRESWICLKKWKIGSDLVYCLMTNWNSFVEQSNLRSGDCI 217
            E    ++  G+  +++D   ++  + LK+W  G +  Y L   WN  V+ + L++GD I
Sbjct: 219 EEDTYNDETMGVGAILVDQRSQKWSVILKRW--GQN--YFLSCGWNDVVKANKLKAGDDI 278

Query: 218 QLWSFRKDGVDPQTGTMASHQLCFAM 241
            LW+FR DGV           LCFAM
Sbjct: 279 CLWAFRCDGV-----------LCFAM 289

[Close]

BLAST of Lag0028994 vs. ExPASy TrEMBL
Match: A0A6J1CX35 (putative B3 domain-containing protein At2g27410 OS=Momordica charantia OX=3673 GN=LOC111015593 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 9.1e-67
Identity = 156/272 (57.35%), Postives = 192/272 (70.59%), Query Frame = 0


Query: 1   MGMG--MNDEGLLLGLPFPNSIIGVHIPRRRRSGSSES--------RPKHE----ACISM 60
           MGMG  +  E L +G+ F +   G+ IP+RRR+GSS S        +PK      A +SM
Sbjct: 1   MGMGTVVELEALKMGICFSDLTNGISIPQRRRTGSSSSSAETSETNKPKPSSSATAPMSM 60

Query: 61  FEAARILIAMANG-----GFCKGEIETNNGKRKKSPTTTK----KQRKKRRKEIKLPNSP 120
            EAA +L+AMA        FCK E+E N+ KRK+ P +      KQRKK+RK ++LP +P
Sbjct: 61  VEAAAVLLAMAYSATDVEAFCKSELEKNSRKRKRDPVSKSEPEMKQRKKKRKAMELPVTP 120

Query: 121 EMPAAMRDRIVEMGGYGIALVIQKTLQDTDMNKNHGRLSLPPKKVKI-DFVTEEERKLLN 180
           EMPAAMRDRIVE+GGYGI LVIQK LQ+TDMNKNHGRLSLP KK++I DFVTEEERKLLN
Sbjct: 121 EMPAAMRDRIVEIGGYGIQLVIQKQLQNTDMNKNHGRLSLPAKKLRIHDFVTEEERKLLN 180

Query: 181 E-QNQKNKKGMKVMIMDAVLRESWICLKKWKIGSDLVYCLMTNWNSFVEQSNLRSGDCIQ 240
           +  N  NKKGM V I+D  L+E  ICLKKWKIGS  +YCLMT WNSFVE++ LRSGD IQ
Sbjct: 181 QHSNNNNKKGMDVPIIDPDLKERRICLKKWKIGSGDLYCLMTQWNSFVEETALRSGDFIQ 240

Query: 241 LWSFRKDGVD--PQTGTMASHQLCFAMVKLDL 246
           LWSFRKD  D   +T  +++ +L  AMVKL++
Sbjct: 241 LWSFRKDEFDEAEETEKISTSRLWLAMVKLEV 272

[Close]

BLAST of Lag0028994 vs. ExPASy TrEMBL
Match: A0A6J1JKC5 (B3 domain-containing protein At2g32645-like OS=Cucurbita maxima OX=3661 GN=LOC111487692 PE=4 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 2.0e-61
Identity = 135/229 (58.95%), Postives = 157/229 (68.56%), Query Frame = 0


Query: 21  IGVHIPRRRRSGSSESRPKHEACISMFEAARILIAMANG-----GFCKGEIETNNGKRKK 80
           +GVHIPRR+R  S  +  KH   ISM E A ILIA +N            + T N K   
Sbjct: 17  LGVHIPRRKRGSSGSAITKHS--ISMMECATILIAFSNSVTHVLPSAYLPLRTTNTK--- 76

Query: 81  SPTTTKKQRKKRRKEIKLPNSPEMPAAMRDRIVEMGGYGIALVIQKTLQDTDMNKNHGRL 140
            P T +K RKKR K+   P +P+MPAAMRDRI EMG Y I L IQK LQDTDMNKNHGRL
Sbjct: 77  -PITERKPRKKRCKQTPFPTTPDMPAAMRDRIGEMGAYQIQLAIQKQLQDTDMNKNHGRL 136

Query: 141 SLPPKKVKIDFVTEEERKLLNEQNQKNKKGMKVMIMDAVLRESWICLKKWKIGSDLVYCL 200
           S P KK+K+DF TEEER++L +Q    KKGM VMI+D +LRES ICLK WKIGS   YCL
Sbjct: 137 SFPAKKLKVDFATEEERRVLKQQENGGKKGMNVMIVDPLLRESSICLKLWKIGSGDSYCL 196

Query: 201 MTNWNSFVEQSNLRSGDCIQLWSFRKDGVDPQTGTMASHQLCFAMVKLD 245
           MT WNS VEQ+  +SGD +QLWSFRKD  + +T TM S +LCFAMVKLD
Sbjct: 197 MTQWNSLVEQNGFKSGDHVQLWSFRKDEFEAETQTMVS-RLCFAMVKLD 238

[Close]

BLAST of Lag0028994 vs. ExPASy TrEMBL
Match: A0A6J1H5Z0 (putative B3 domain-containing protein At3g24850 OS=Cucurbita moschata OX=3662 GN=LOC111460418 PE=4 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 4.4e-61
Identity = 136/243 (55.97%), Postives = 161/243 (66.26%), Query Frame = 0


Query: 21  IGVHIPRRRRSGSSESRPKHEACISMFEAARILIAMANG-----GFCKGEIETNNGK--- 80
           +GVHIPRR+R  S  +  K    ISM E A ILIA +N            + T NGK   
Sbjct: 17  LGVHIPRRKRGSSGSAITKRS--ISMMECATILIAFSNSVTHVLPSAYLPLRTTNGKPIT 76

Query: 81  -----------RKKSPTTTKKQRKKRRKEIKLPNSPEMPAAMRDRIVEMGGYGIALVIQK 140
                       ++ P T +K RKKR K+   P +P+MPAAMRDRI EMG Y I L IQK
Sbjct: 77  NTKPITKRKPITERKPITERKPRKKRCKQTPFPTTPDMPAAMRDRIGEMGAYQIQLAIQK 136

Query: 141 TLQDTDMNKNHGRLSLPPKKVKIDFVTEEERKLLNEQNQKNKKGMKVMIMDAVLRESWIC 200
            LQDTDMNKNHGRLS P KK+K+DF TEEER++L +Q  K KKGM VMI+D +LRES IC
Sbjct: 137 QLQDTDMNKNHGRLSFPAKKLKVDFATEEERRVLKQQENKGKKGMNVMIVDPLLRESSIC 196

Query: 201 LKKWKIGSDLVYCLMTNWNSFVEQSNLRSGDCIQLWSFRKDGVDPQTGTMASHQLCFAMV 245
           LK WKIGS   YCLMT WNS VEQ+  +SGD +QLWSFRKD ++ +T TM S +LCFAMV
Sbjct: 197 LKLWKIGSGDSYCLMTQWNSLVEQNGFKSGDHVQLWSFRKDELEAETQTMVS-RLCFAMV 256

[Close]

BLAST of Lag0028994 vs. ExPASy TrEMBL
Match: A0A5A7VC33 (Putative B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005250 PE=4 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 2.0e-58
Identity = 138/239 (57.74%), Postives = 168/239 (70.29%), Query Frame = 0


Query: 22  GVHIPRRRRSGSSESRPKHE-ACISMFEAARILIAMANGGFCKGEIETNNGKRKKSPTT- 81
           GVHIPRR++SGS ES+ + E A ISM EAA+IL+AMANGGFC+        KRKKSPT  
Sbjct: 22  GVHIPRRKKSGSVESKKEDEGASISMVEAAKILLAMANGGFCE-------RKRKKSPTVK 81

Query: 82  ------TKKQRKK------RRKEIKLPNS-PEMPAAMRDRIVEMGGYGIALVIQKTLQDT 141
                 T KQRKK      R+K ++L  S PEMPA+MRDRIV  GGY I LVIQK L+++
Sbjct: 82  QSKKSPTAKQRKKSPSEKQRKKSMELEESFPEMPASMRDRIVGNGGYEIQLVIQKQLEES 141

Query: 142 DMNKNHGRLSLPPKKVKIDFVTEEERKLLNEQN---QKNKKGMKVMIMDAVLRESWICLK 201
           D+++NHGRL LP  KVK +FV EEER +L E+N   +KNKKG++V +M   LRES +CLK
Sbjct: 142 DVSRNHGRLCLPATKVKTEFVREEERNILEEENNGKKKNKKGLEVGVMGDDLRESSMCLK 201

Query: 202 KWKIGSDLVYCLMTNWNSFVEQSNLRSGDCIQLWSFRKDGVDPQTGTMASHQLCFAMVK 243
           KWKIGS   YCLM NWN FVE++ LRSGD IQLWSFR + +        S QLCFA+VK
Sbjct: 202 KWKIGSGKFYCLMKNWNCFVEENGLRSGDYIQLWSFRNNNI--------SDQLCFAIVK 245

[Close]

BLAST of Lag0028994 vs. ExPASy TrEMBL
Match: A0A1S3BD17 (putative B3 domain-containing protein At3g49610 OS=Cucumis melo OX=3656 GN=LOC103488551 PE=4 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 2.0e-58
Identity = 138/239 (57.74%), Postives = 168/239 (70.29%), Query Frame = 0


Query: 22  GVHIPRRRRSGSSESRPKHE-ACISMFEAARILIAMANGGFCKGEIETNNGKRKKSPTT- 81
           GVHIPRR++SGS ES+ + E A ISM EAA+IL+AMANGGFC+        KRKKSPT  
Sbjct: 18  GVHIPRRKKSGSVESKKEDEGASISMVEAAKILLAMANGGFCE-------RKRKKSPTVK 77

Query: 82  ------TKKQRKK------RRKEIKLPNS-PEMPAAMRDRIVEMGGYGIALVIQKTLQDT 141
                 T KQRKK      R+K ++L  S PEMPA+MRDRIV  GGY I LVIQK L+++
Sbjct: 78  QSKKSPTAKQRKKSPSEKQRKKSMELEESFPEMPASMRDRIVGNGGYEIQLVIQKQLEES 137

Query: 142 DMNKNHGRLSLPPKKVKIDFVTEEERKLLNEQN---QKNKKGMKVMIMDAVLRESWICLK 201
           D+++NHGRL LP  KVK +FV EEER +L E+N   +KNKKG++V +M   LRES +CLK
Sbjct: 138 DVSRNHGRLCLPATKVKTEFVREEERNILEEENNGKKKNKKGLEVGVMGDDLRESSMCLK 197

Query: 202 KWKIGSDLVYCLMTNWNSFVEQSNLRSGDCIQLWSFRKDGVDPQTGTMASHQLCFAMVK 243
           KWKIGS   YCLM NWN FVE++ LRSGD IQLWSFR + +        S QLCFA+VK
Sbjct: 198 KWKIGSGKFYCLMKNWNCFVEENGLRSGDYIQLWSFRNNNI--------SDQLCFAIVK 241

[Close]

BLAST of Lag0028994 vs. TAIR 10
Match: AT2G31420.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 73.6 bits (179), Expect = 2.6e-13
Identity = 48/132 (36.36%), Postives = 74/132 (56.06%), Query Frame = 0


Query: 112 GYGIALVI-QKTLQDTDMNKNHGRLSLPPKKV-KIDFVTEEERKLLNEQNQKNKK-GMKV 171
           GY   L+I ++ L  TD+ KN G LS+   K+ K DF+TE+E + L+E   K K+ G+KV
Sbjct: 73  GYDPKLIIKRRVLCTTDLRKNQGCLSMHLSKLEKSDFLTEDETRFLDEDFLKAKRDGLKV 132

Query: 172 MIMDAVLRESWICLKKWKIGSDLVYCLMTNWNSFVEQSNLRSGDCIQLWSFRKDGVDPQT 231
            ++D    +  + LKKW   +   Y L   WN+ +++   +  D I++WSFR DG     
Sbjct: 133 FLVDPESDKHVVYLKKWNGNTVWKYVLSHGWNNVIDKKIFKVNDVIEIWSFR-DG----- 192

Query: 232 GTMASHQLCFAM 241
               S +LCFA+
Sbjct: 193 ----SGKLCFAL 194

[Close]

BLAST of Lag0028994 vs. TAIR 10
Match: AT5G24050.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 73.2 bits (178), Expect = 3.4e-13
Identity = 46/131 (35.11%), Postives = 70/131 (53.44%), Query Frame = 0


Query: 117 LVIQKTLQDTDMNKNHGRLSLP-PKKVKIDFVTEEERKLLNEQ-NQKNKKGMKVMIMDAV 176
           L+ +KTL  TD+N    RLS+P    ++ DF+T  E  ++NE  N  NK G+  +++D  
Sbjct: 218 LIFEKTLFVTDVNPTKNRLSMPFNNLLRNDFLTSVESIIINEDINNNNKIGVGAILVDQR 277

Query: 177 LRESWICLKKWKIGSD-----LVYCLMTNWNSFVEQSNLRSGDCIQLWSFRKDGVDPQTG 236
            ++  + LK+W++  +       Y L+  WN  VE + L+ GD I LWSFR  G+     
Sbjct: 278 CKKWGVMLKRWEMKKESGKGSWSYNLICGWNDIVEANRLKEGDNITLWSFRCCGI----- 337

Query: 237 TMASHQLCFAM 241
                 LCFAM
Sbjct: 338 ------LCFAM 337

[Close]

BLAST of Lag0028994 vs. TAIR 10
Match: AT3G24850.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 69.7 bits (169), Expect = 3.7e-12
Identity = 45/131 (34.35%), Postives = 69/131 (52.67%), Query Frame = 0


Query: 117 LVIQKTLQDTDMNKNHGRLSLP-PKKVKIDFVTEEERKLLNEQNQKNKK-GMKVMIMDAV 176
           L+ +KTL  TD+N    RLS+P    ++ DF+T  E +++ E    NKK G+  +++D  
Sbjct: 228 LIFEKTLFVTDINPTQNRLSMPFNNLLQNDFLTSVESRIIKEDINNNKKIGVGAILVDQR 287

Query: 177 LRESWICLKKWKIGSD-----LVYCLMTNWNSFVEQSNLRSGDCIQLWSFRKDGVDPQTG 236
             +  + LK+W++  +       Y L+  WN  VE + L+ GD I +WSFR  GV     
Sbjct: 288 SVKWGVMLKRWELKKESGKGSWNYNLICGWNDVVEANGLKEGDNISVWSFRCRGV----- 347

Query: 237 TMASHQLCFAM 241
                 LCFAM
Sbjct: 348 ------LCFAM 347

[Close]

BLAST of Lag0028994 vs. TAIR 10
Match: AT1G05930.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 68.9 bits (167), Expect = 6.3e-12
Identity = 48/146 (32.88%), Postives = 77/146 (52.74%), Query Frame = 0


Query: 98  EMPAAMRDRIVEMGGYGIALVIQKTLQDTD-MNKNHGRLSLP-PKKVKIDFVTEEERKLL 157
           EM   +R  + +M    + L+++KTL  TD +   HGR S+   + +  DF+  EER +L
Sbjct: 159 EMRWWLRQVMKDMRAEDLTLILEKTLSTTDVITTTHGRFSMHFNRLISNDFLKPEERSIL 218

Query: 158 NEQNQKNK-KGMKVMIMDAVLRESWICLKKWKIGSDLVYCLMTNWNSFVEQSNLRSGDCI 217
            E    ++  G+  +++D   ++  + LK+W  G +  Y L   WN  V+ + L++GD I
Sbjct: 219 EEDTYNDETMGVGAILVDQRSQKWSVILKRW--GQN--YFLSCGWNDVVKANKLKAGDDI 278

Query: 218 QLWSFRKDGVDPQTGTMASHQLCFAM 241
            LW+FR DGV           LCFAM
Sbjct: 279 CLWAFRCDGV-----------LCFAM 289

[Close]

BLAST of Lag0028994 vs. TAIR 10
Match: AT2G24670.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 62.4 bits (150), Expect = 5.9e-10
Identity = 35/107 (32.71%), Postives = 61/107 (57.01%), Query Frame = 0


Query: 118 VIQKTLQDTDMNKNHGRLSLPPKK-VKIDFVTEEERKLLNEQ-NQKNKKGMKVMIMDAVL 177
           +I K L  TD++ +  RLS+P  + V+++F+  EE++ + E  N+  K+G+  +++D+ L
Sbjct: 132 IIDKELFQTDVDPHQSRLSIPVSQIVELEFLNHEEKRAIEEDANRVRKEGVDAILVDSHL 191

Query: 178 RESWICLKKWKIGSDLVYCLMTNWNSFVEQSNLRSGDCIQLWSFRKD 223
           RE  + L+   +   L+Y L+  WN  V    L     I+LWSF  D
Sbjct: 192 REFPVNLRLRDMRGILLYNLVAGWNQVVTDCLLEENTNIRLWSFHAD 238

The following BLAST results are available for this feature:

BLAST of Lag0028994 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146365.1	1.9e-66	57.35	putative B3 domain-containing protein At2g27410 [Momordica charantia]	[more]
XP_022990807.1	4.1e-61	58.95	B3 domain-containing protein At2g32645-like [Cucurbita maxima] >XP_022990957.1 B...	[more]
XP_022959448.1	9.0e-61	55.97	putative B3 domain-containing protein At3g24850 [Cucurbita moschata]	[more]
XP_038875653.1	1.2e-60	58.00	B3 domain-containing protein At1g05920-like [Benincasa hispida]	[more]
XP_023540870.1	3.4e-60	55.97	putative B3 domain-containing protein At3g24850 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0028994 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SIC7	3.6e-12	36.36	B3 domain-containing protein At2g31420 OS=Arabidopsis thaliana OX=3702 GN=At2g31...	[more]
Q9FLV7	4.7e-12	35.11	B3 domain-containing protein At5g24050 OS=Arabidopsis thaliana OX=3702 GN=At5g24...	[more]
Q9LSF7	1.8e-11	32.82	B3 domain-containing protein At3g25182 OS=Arabidopsis thaliana OX=3702 GN=At3g25...	[more]
Q9LRX6	5.2e-11	34.35	Putative B3 domain-containing protein At3g24850 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9MA32	8.9e-11	32.88	B3 domain-containing protein At1g05930 OS=Arabidopsis thaliana OX=3702 GN=At1g05...	[more]

BLAST of Lag0028994 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CX35	9.1e-67	57.35	putative B3 domain-containing protein At2g27410 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1JKC5	2.0e-61	58.95	B3 domain-containing protein At2g32645-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1H5Z0	4.4e-61	55.97	putative B3 domain-containing protein At3g24850 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A5A7VC33	2.0e-58	57.74	Putative B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3BD17	2.0e-58	57.74	putative B3 domain-containing protein At3g49610 OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of Lag0028994 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G31420.1	2.6e-13	36.36	Domain of unknown function (DUF313)	[more]
AT5G24050.1	3.4e-13	35.11	Domain of unknown function (DUF313)	[more]
AT3G24850.1	3.7e-12	34.35	Domain of unknown function (DUF313)	[more]
AT1G05930.1	6.3e-12	32.88	Domain of unknown function (DUF313)	[more]
AT2G24670.1	5.9e-10	32.71	Domain of unknown function (DUF313)	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005508	B3 domain-containing protein At2g31720-like	PFAM	PF03754	DUF313	coord: 98..204 e-value: 6.9E-19 score: 68.1
IPR005508	B3 domain-containing protein At2g31720-like	PANTHER	PTHR31541	B3 DOMAIN PLANT PROTEIN-RELATED	coord: 60..243
IPR015300	DNA-binding pseudobarrel domain superfamily	GENE3D	2.40.330.10		coord: 113..234 e-value: 1.5E-16 score: 62.2
IPR015300	DNA-binding pseudobarrel domain superfamily	SUPERFAMILY	101936	DNA-binding pseudobarrel domain	coord: 118..222
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 65..97
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 75..90
None	No IPR available	PANTHER	PTHR31541:SF25	DOMAIN PROTEIN, PUTATIVE (DUF313)-RELATED	coord: 60..243
IPR003340	B3 DNA binding domain	CDD	cd10017	B3_DNA	coord: 119..224 e-value: 1.2661E-6 score: 43.8557

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0028994.1	Lag0028994.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding