Lag0028958 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0028958
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptiongibberellin 2-beta-dioxygenase
Locationchr8: 33422114 .. 33423042 (-)
RNA-Seq ExpressionLag0028958
SyntenyLag0028958
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGTTCTGTCACAGCCAACATTGGGTTTAGACCAATATTCTCTTCTTCGAACAAGATTCAAACCCACCGCCTCCTTCACCGAAATTCATGAAATCGACCTCTCAGACCCCAACGCCCAGTCCCACATCGTCGAGGCCTGTGAAGAATTCGGCTTCTTTAAGCTCGTAAACCATCGAGTTCCCGTTGAATTGATGGCCAAACTCGAAGAAGAAGCCCTTTGTTTTATCAAACATTCTCAGTCCGAGAAAGATAAAGCTCGCCCTTCTGACCCTTTAGGCTATGGCTGCAAAAATATCGGCTCTAATGGCGATAAGGGTTGGATTAAATACCTCCTCCCCACCGCCAATCCTCTCCCCATTTTCACCCAAAACTCAGAATATTTCCTGTAAGTTGCAAAACAAAAAAAATAGAGCACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTATTTATTTATTTAAGAATCCACTTAGATTTTGAAAAAGTATTATTTATAAAAGAATAAAATCTTTCCTTAACATCATTTTCTCGTGTTACTGATTTTGGGCAAATTTTCTTGTAGCGCCGCCGCGAAGGAGTATGTCACGGCAGTGAAGAAGCTAAGCTACGAAGTGGCGGAATTGATAGTAGAGGGATTGAAAATCGAGCCAAGAAACGCAATAAGCAAGCTCCTAAACGACGAGAAAAGCGACTGTTGTTTCAGAGTGAACCACTACCCGCCATGTCCAGACCTCCAAGCTGTAGGCGGAAGTGGAAGGAACATGATCGGATTCGGGGAACACACAGACCCACAAATCATATCGCTGCTAAGATCAAACAACTCGACCGACCTACAAATCTGCCTCAAAGACAGCGCGTGGGTGTCAGTGCCGCCGGACGCGGCGACGATCTTCGTGAACGTTGGCGACGCTCTGTAG

mRNA sequence

ATGGTTGTTCTGTCACAGCCAACATTGGGTTTAGACCAATATTCTCTTCTTCGAACAAGATTCAAACCCACCGCCTCCTTCACCGAAATTCATGAAATCGACCTCTCAGACCCCAACGCCCAGTCCCACATCGTCGAGGCCTGTGAAGAATTCGGCTTCTTTAAGCTCGTAAACCATCGAGTTCCCGTTGAATTGATGGCCAAACTCGAAGAAGAAGCCCTTTGTTTTATCAAACATTCTCAGTCCGAGAAAGATAAAGCTCGCCCTTCTGACCCTTTAGGCTATGGCTGCAAAAATATCGGCTCTAATGGCGATAAGGGTTGGATTAAATACCTCCTCCCCACCGCCAATCCTCTCCCCATTTTCACCCAAAACTCAGAATATTTCCTCGCCGCCGCGAAGGAGTATGTCACGGCAGTGAAGAAGCTAAGCTACGAAGTGGCGGAATTGATAGTAGAGGGATTGAAAATCGAGCCAAGAAACGCAATAAGCAAGCTCCTAAACGACGAGAAAAGCGACTGTTGTTTCAGAGTGAACCACTACCCGCCATGTCCAGACCTCCAAGCTGTAGGCGGAAGTGGAAGGAACATGATCGGATTCGGGGAACACACAGACCCACAAATCATATCGCTGCTAAGATCAAACAACTCGACCGACCTACAAATCTGCCTCAAAGACAGCGCGTGGGTGTCAGTGCCGCCGGACGCGGCGACGATCTTCGTGAACGTTGGCGACGCTCTGTAG

Coding sequence (CDS)

ATGGTTGTTCTGTCACAGCCAACATTGGGTTTAGACCAATATTCTCTTCTTCGAACAAGATTCAAACCCACCGCCTCCTTCACCGAAATTCATGAAATCGACCTCTCAGACCCCAACGCCCAGTCCCACATCGTCGAGGCCTGTGAAGAATTCGGCTTCTTTAAGCTCGTAAACCATCGAGTTCCCGTTGAATTGATGGCCAAACTCGAAGAAGAAGCCCTTTGTTTTATCAAACATTCTCAGTCCGAGAAAGATAAAGCTCGCCCTTCTGACCCTTTAGGCTATGGCTGCAAAAATATCGGCTCTAATGGCGATAAGGGTTGGATTAAATACCTCCTCCCCACCGCCAATCCTCTCCCCATTTTCACCCAAAACTCAGAATATTTCCTCGCCGCCGCGAAGGAGTATGTCACGGCAGTGAAGAAGCTAAGCTACGAAGTGGCGGAATTGATAGTAGAGGGATTGAAAATCGAGCCAAGAAACGCAATAAGCAAGCTCCTAAACGACGAGAAAAGCGACTGTTGTTTCAGAGTGAACCACTACCCGCCATGTCCAGACCTCCAAGCTGTAGGCGGAAGTGGAAGGAACATGATCGGATTCGGGGAACACACAGACCCACAAATCATATCGCTGCTAAGATCAAACAACTCGACCGACCTACAAATCTGCCTCAAAGACAGCGCGTGGGTGTCAGTGCCGCCGGACGCGGCGACGATCTTCGTGAACGTTGGCGACGCTCTGTAG

Protein sequence

MVVLSQPTLGLDQYSLLRTRFKPTASFTEIHEIDLSDPNAQSHIVEACEEFGFFKLVNHRVPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTANPLPIFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSDCCFRVNHYPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVPPDAATIFVNVGDAL
Homology
BLAST of Lag0028958 vs. NCBI nr
Match: AUB30295.1 (gibberellin 2-beta-dioxygenase 8-like protein [Luffa aegyptiaca])

HSP 1 Score: 462.2 bits (1188), Expect = 2.8e-126
Identity = 226/247 (91.50%), Postives = 232/247 (93.93%), Query Frame = 0

Query: 1   MVVLSQPTLGLDQYSLLRTRFKPTASFTEIHEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQP LGLDQYSLLRT  KPTASFTEI EIDLSDPNAQSHIVEACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60

Query: 61  VPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTANPLP 120
           VP+ELM KLEEEALCF K SQSEKDKARP DPLGYGCKNIGSNGDKGWI+YLL TANPLP
Sbjct: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120

Query: 121 IFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           IF+QNSE FLAAAKEYVTAVKKLS EVAELI EGLKIEPRNAISKLLNDEKSDCCFRVNH
Sbjct: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180

Query: 181 YPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVPPDAATIF 240
           YPPCPDLQA+GGSGRNMIGFGEHTDPQIISLLRSNNST LQICL+D AWVSVPPDAA IF
Sbjct: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240

Query: 241 VNVGDAL 248
           VNVGD+L
Sbjct: 241 VNVGDSL 247

BLAST of Lag0028958 vs. NCBI nr
Match: XP_038883200.1 (gibberellin 2-beta-dioxygenase-like [Benincasa hispida])

HSP 1 Score: 408.3 bits (1048), Expect = 4.7e-110
Identity = 198/247 (80.16%), Postives = 220/247 (89.07%), Query Frame = 0

Query: 1   MVVLSQPTLGLDQYSLLRTRFKPTASFTEIHEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQPTL L+QYSLLR+  KPTASF +I EIDLSDPNA+SHI++ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHR 60

Query: 61  VPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTANPLP 120
           VPVELM KLE+E+LCF K S+SEKDKARP  PLGYGCKNIGSNGDKGWI+YLL  ANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 IFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           I +QNS  FL AA+EYV AVKKLS EV ELI EGLK+EPRN ISKLLNDEK+DCCFRVNH
Sbjct: 121 ILSQNS--FLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNH 180

Query: 181 YPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVPPDAATIF 240
           YPPCP++Q++G  GRNMIGFGEHTDPQIIS+LRSNNS+ LQICL+D AWVSVPPDA  +F
Sbjct: 181 YPPCPEMQSLG--GRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALF 240

Query: 241 VNVGDAL 248
           VNVGDAL
Sbjct: 241 VNVGDAL 243

BLAST of Lag0028958 vs. NCBI nr
Match: XP_022977872.1 (gibberellin 2-beta-dioxygenase [Cucurbita maxima])

HSP 1 Score: 399.4 bits (1025), Expect = 2.2e-107
Identity = 194/247 (78.54%), Postives = 215/247 (87.04%), Query Frame = 0

Query: 1   MVVLSQPTLGLDQYSLLRTRFKPTASFTEIHEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQP L L++YSLLR+  KP AS  +I EIDLSDPNA+SH+V+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTANPLP 120
           VPVELM KLE+E+LCF K SQSEKDKARP DPLGYG KNIGSNGDKGWI+YLL  ANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 IFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           +F+Q S+ FL+AA EYVTAVKKLS EV ELI EGLKIEP+NAIS+L+NDEK+DC FRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVPPDAATIF 240
           YPPCP++QAV  SGRNMIGFGEHTDPQIISLLRSNNST LQIC +D  WVSVPPD    F
Sbjct: 181 YPPCPEMQAV--SGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFF 240

Query: 241 VNVGDAL 248
           VNVGDAL
Sbjct: 241 VNVGDAL 245

BLAST of Lag0028958 vs. NCBI nr
Match: CBL59329.1 (Gibberellin 2-oxidase [Cucurbita maxima])

HSP 1 Score: 399.4 bits (1025), Expect = 2.2e-107
Identity = 194/247 (78.54%), Postives = 215/247 (87.04%), Query Frame = 0

Query: 1   MVVLSQPTLGLDQYSLLRTRFKPTASFTEIHEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQP L L++YSLLR+  KP AS  +I EIDLSDPNA+SH+V+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTANPLP 120
           VPVELM KLE+E+LCF K SQSEKDKARP DPLGYG KNIGSNGDKGWI+YLL  ANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 IFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           +F+Q S+ FL+AA EYVTAVKKLS EV ELI EGLKIEP+NAIS+L+NDEK+DC FRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVPPDAATIF 240
           YPPCP++QAV  SGRNMIGFGEHTDPQIISLLRSNNST LQIC +D  WVSVPPD    F
Sbjct: 181 YPPCPEMQAV--SGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFF 240

Query: 241 VNVGDAL 248
           VNVGDAL
Sbjct: 241 VNVGDAL 245

BLAST of Lag0028958 vs. NCBI nr
Match: XP_008439633.1 (PREDICTED: gibberellin 2-beta-dioxygenase-like [Cucumis melo])

HSP 1 Score: 396.7 bits (1018), Expect = 1.4e-106
Identity = 196/247 (79.35%), Postives = 215/247 (87.04%), Query Frame = 0

Query: 1   MVVLSQPTLGLDQYSLLRTRFKPTASFTEIHEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQPTL L+QYSLLR+  KPTASF +I EIDLSDPNA+ HIV+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  VPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTANPLP 120
           VP+ELM KLE+E+LCF K S+SEKDKARP  PLGYG KNIGSNGDKGWI+YLL  ANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 IFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           IF+ +   FL AA EYVTAVKKLS EV ELI EGLK+E RNAISKLLNDEK+DCCFRVNH
Sbjct: 121 IFSHD---FLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNH 180

Query: 181 YPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVPPDAATIF 240
           YPPCP++Q  G SGRNMIGFGEHTDPQIIS+LRSNNST LQICL+D AWVSVPPD   +F
Sbjct: 181 YPPCPEMQ--GLSGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALF 240

Query: 241 VNVGDAL 248
           VNVGDAL
Sbjct: 241 VNVGDAL 242

BLAST of Lag0028958 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 2.8e-81
Identity = 152/254 (59.84%), Postives = 185/254 (72.83%), Query Frame = 0

Query: 1   MVVLSQPTLGLDQYSLLRTRFKPTASFTEIHEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQP   L+Q+ LL+  FK T  FT I  +DL+ P+A++ IV AC +FGFFKLVNH 
Sbjct: 1   MVVLSQP--ALNQFFLLKP-FKSTPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHG 60

Query: 61  VPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTANP-- 120
           VP+ELMA LE EAL F K SQSEKD+A P DP GYG K IG NGD GW++YLL   NP  
Sbjct: 61  VPLELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDV 120

Query: 121 -----LPIFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSD 180
                L IF +N  +F A  + Y+TAVK + Y V EL+ EGL I  RN +S+LL DEKSD
Sbjct: 121 ISPKSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSD 180

Query: 181 CCFRVNHYPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVP 240
            CFR+NHYPPCP++QA+    RN++GFGEHTDPQIIS+LRSN+++ LQICL D  WVSVP
Sbjct: 181 SCFRLNHYPPCPEVQAL---NRNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVP 240

Query: 241 PDAATIFVNVGDAL 248
           PD  + F+NVGDAL
Sbjct: 241 PDQTSFFINVGDAL 248

BLAST of Lag0028958 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 3.6e-68
Identity = 125/250 (50.00%), Postives = 181/250 (72.40%), Query Frame = 0

Query: 1   MVVLSQPTLGLDQYSLLRTRFKPTASFTEIHEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MV+LS+PT   +QY+ +R     T S + I  +DLS P+A++ IV+ACE+FGFFK++NH 
Sbjct: 1   MVLLSKPT--SEQYTYVRNNMPITFS-SSIPLVDLSKPDAKTLIVKACEDFGFFKVINHG 60

Query: 61  VPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTANP-- 120
           +P++ +++LE EA  F    Q+EK+KA P++P GYG K IG NGD GWI+YLL T N   
Sbjct: 61  IPLDAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIEYLLLTTNQDH 120

Query: 121 -LPIFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSDCCFR 180
              ++ ++ + F    K+Y  A++ ++ E+ +L+ EGLKI+P+N  SKL+ D++SDC FR
Sbjct: 121 NFSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLFR 180

Query: 181 VNHYPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVPPDAA 240
           VNHYP CP+L     +G N+IGFGEHTDPQIIS+LRSNN++  QI L+D +W+SVPPD +
Sbjct: 181 VNHYPACPELAI---NGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHS 240

Query: 241 TIFVNVGDAL 248
           + F+NVGD+L
Sbjct: 241 SFFINVGDSL 244

BLAST of Lag0028958 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 5.7e-66
Identity = 130/256 (50.78%), Postives = 172/256 (67.19%), Query Frame = 0

Query: 1   MVVLSQPTLGLDQYSLLRTRFKPTASFT--EIHEIDLSDPNAQSHIVEACEEFGFFKLVN 60
           MVVL QP + LD +  L   +KP    T   I  ++L+DP A++ IV+ACEEFGFFK+VN
Sbjct: 1   MVVLPQP-VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTANP 120
           H V  ELM +LE+EA+ F    QS K++A P +P GYG K IG NGD GWI+YLL  ANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 -------LPIFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEK 180
                    +F Q  + F  + +EY+  +K++SY+V E++ E L IEPR+ +SK+L DEK
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 SDCCFRVNHYPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVS 240
           SD C R+NHYP   +           +GFGEHTDPQIIS+LRSNN+  LQIC+KD +WV+
Sbjct: 181 SDSCLRLNHYPAAEE----EAEKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVA 240

Query: 241 VPPDAATIFVNVGDAL 248
           VPPD ++ F+NVGDAL
Sbjct: 241 VPPDHSSFFINVGDAL 251

BLAST of Lag0028958 vs. ExPASy Swiss-Prot
Match: Q8LEA2 (Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 SV=2)

HSP 1 Score: 231.5 bits (589), Expect = 1.0e-59
Identity = 114/232 (49.14%), Postives = 157/232 (67.67%), Query Frame = 0

Query: 23  PTASFTEIHEIDLSDPNAQSHIVEACEEFGFFKLVNHRVPVELMAKLEEEALCFIKHSQS 82
           P + F+ I  ID+SDP ++  +V+ACE+FGFFK++NH V  EL++ LE E + F    +S
Sbjct: 11  PKSGFSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKS 70

Query: 83  EKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTAN------PL-PIFTQNSEYFLAAAKE 142
           EK +     P GYG   IG NGD GW++YLL  AN      PL P   ++   F  A +E
Sbjct: 71  EKTQV-AGYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEE 130

Query: 143 YVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSDCCFRVNHYPPCPDLQAVGGSGR 202
           Y T+V+K++++V E I +GL I+PRN +SKL++D+ +D   R+NHYPPCP        G+
Sbjct: 131 YTTSVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGK 190

Query: 203 NMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVPPDAATIFVNVGDAL 248
           N+IGFGEHTDPQIIS+LRSNN++ LQI L D +W+SVPPD  + F NVGD+L
Sbjct: 191 NVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSL 241

BLAST of Lag0028958 vs. ExPASy Swiss-Prot
Match: O64692 (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 4.1e-56
Identity = 114/254 (44.88%), Postives = 157/254 (61.81%), Query Frame = 0

Query: 1   MVVLSQPTLGLDQYSLLRTRFKPTASFTEIHEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MV++ QP    D    +  + KP      +  IDL+D +A++ IV+ACEEFGFFK++NH 
Sbjct: 1   MVIVLQPA-SFDSNLYVNPKCKPRPVLIPV--IDLTDSDAKTQIVKACEEFGFFKVINHG 60

Query: 61  VPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTAN--- 120
           V  +L+ +LE+EA+ F     S KDKA P DP GYG K IG NGD GW++Y+L  AN   
Sbjct: 61  VRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCL 120

Query: 121 ----PLPIFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSD 180
                  IF      F  A +EY+  +K++S +  E++ E LKIEP+  +S+L+  ++SD
Sbjct: 121 ESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESD 180

Query: 181 CCFRVNHYPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVP 240
            C R+NHYP   +        +  IGFGEHTDPQ+ISLLRSN++  LQIC+KD  WV V 
Sbjct: 181 SCLRMNHYPEKEETPV-----KEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVT 240

Query: 241 PDAATIFVNVGDAL 248
           PD ++ FV VGD L
Sbjct: 241 PDHSSFFVLVGDTL 246

BLAST of Lag0028958 vs. ExPASy TrEMBL
Match: A0A2H4V3T7 (Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 1.3e-126
Identity = 226/247 (91.50%), Postives = 232/247 (93.93%), Query Frame = 0

Query: 1   MVVLSQPTLGLDQYSLLRTRFKPTASFTEIHEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQP LGLDQYSLLRT  KPTASFTEI EIDLSDPNAQSHIVEACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60

Query: 61  VPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTANPLP 120
           VP+ELM KLEEEALCF K SQSEKDKARP DPLGYGCKNIGSNGDKGWI+YLL TANPLP
Sbjct: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120

Query: 121 IFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           IF+QNSE FLAAAKEYVTAVKKLS EVAELI EGLKIEPRNAISKLLNDEKSDCCFRVNH
Sbjct: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180

Query: 181 YPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVPPDAATIF 240
           YPPCPDLQA+GGSGRNMIGFGEHTDPQIISLLRSNNST LQICL+D AWVSVPPDAA IF
Sbjct: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240

Query: 241 VNVGDAL 248
           VNVGD+L
Sbjct: 241 VNVGDSL 247

BLAST of Lag0028958 vs. ExPASy TrEMBL
Match: A0A6J1IJN1 (gibberellin 2-beta-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111478033 PE=3 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 1.1e-107
Identity = 194/247 (78.54%), Postives = 215/247 (87.04%), Query Frame = 0

Query: 1   MVVLSQPTLGLDQYSLLRTRFKPTASFTEIHEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQP L L++YSLLR+  KP AS  +I EIDLSDPNA+SH+V+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTANPLP 120
           VPVELM KLE+E+LCF K SQSEKDKARP DPLGYG KNIGSNGDKGWI+YLL  ANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 IFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           +F+Q S+ FL+AA EYVTAVKKLS EV ELI EGLKIEP+NAIS+L+NDEK+DC FRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVPPDAATIF 240
           YPPCP++QAV  SGRNMIGFGEHTDPQIISLLRSNNST LQIC +D  WVSVPPD    F
Sbjct: 181 YPPCPEMQAV--SGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFF 240

Query: 241 VNVGDAL 248
           VNVGDAL
Sbjct: 241 VNVGDAL 245

BLAST of Lag0028958 vs. ExPASy TrEMBL
Match: I0J1J0 (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox2 PE=2 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 1.1e-107
Identity = 194/247 (78.54%), Postives = 215/247 (87.04%), Query Frame = 0

Query: 1   MVVLSQPTLGLDQYSLLRTRFKPTASFTEIHEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQP L L++YSLLR+  KP AS  +I EIDLSDPNA+SH+V+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTANPLP 120
           VPVELM KLE+E+LCF K SQSEKDKARP DPLGYG KNIGSNGDKGWI+YLL  ANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 IFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           +F+Q S+ FL+AA EYVTAVKKLS EV ELI EGLKIEP+NAIS+L+NDEK+DC FRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVPPDAATIF 240
           YPPCP++QAV  SGRNMIGFGEHTDPQIISLLRSNNST LQIC +D  WVSVPPD    F
Sbjct: 181 YPPCPEMQAV--SGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFF 240

Query: 241 VNVGDAL 248
           VNVGDAL
Sbjct: 241 VNVGDAL 245

BLAST of Lag0028958 vs. ExPASy TrEMBL
Match: A0A1S3AZ81 (gibberellin 2-beta-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103484366 PE=3 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 6.9e-107
Identity = 196/247 (79.35%), Postives = 215/247 (87.04%), Query Frame = 0

Query: 1   MVVLSQPTLGLDQYSLLRTRFKPTASFTEIHEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQPTL L+QYSLLR+  KPTASF +I EIDLSDPNA+ HIV+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  VPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTANPLP 120
           VP+ELM KLE+E+LCF K S+SEKDKARP  PLGYG KNIGSNGDKGWI+YLL  ANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 IFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           IF+ +   FL AA EYVTAVKKLS EV ELI EGLK+E RNAISKLLNDEK+DCCFRVNH
Sbjct: 121 IFSHD---FLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNH 180

Query: 181 YPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVPPDAATIF 240
           YPPCP++Q  G SGRNMIGFGEHTDPQIIS+LRSNNST LQICL+D AWVSVPPD   +F
Sbjct: 181 YPPCPEMQ--GLSGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALF 240

Query: 241 VNVGDAL 248
           VNVGDAL
Sbjct: 241 VNVGDAL 242

BLAST of Lag0028958 vs. ExPASy TrEMBL
Match: A0A5A7UGB1 (Gibberellin 2-beta-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009030 PE=3 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 9.0e-107
Identity = 196/247 (79.35%), Postives = 215/247 (87.04%), Query Frame = 0

Query: 1   MVVLSQPTLGLDQYSLLRTRFKPTASFTEIHEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQPTL L+QYSLLR+  KPTASF +I EIDLSDPNA+ HIV+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  VPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTANPLP 120
           VP+ELM KLE+E+LCF K S+SEKDKARP  PLGYG KNIGSNGDKGWI+YLL  ANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 IFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           IF+ +   FL AA EYVTAVKKLS EV ELI EGLK+E RNAISKLLNDEK+DCCFRVNH
Sbjct: 121 IFSHD---FLRAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNH 180

Query: 181 YPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVPPDAATIF 240
           YPPCP++Q  G SGRNMIGFGEHTDPQIIS+LRSNNST LQICL+D AWVSVPPD   +F
Sbjct: 181 YPPCPEMQ--GLSGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALF 240

Query: 241 VNVGDAL 248
           VNVGDAL
Sbjct: 241 VNVGDAL 242

BLAST of Lag0028958 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 252.3 bits (643), Expect = 4.0e-67
Identity = 130/256 (50.78%), Postives = 172/256 (67.19%), Query Frame = 0

Query: 1   MVVLSQPTLGLDQYSLLRTRFKPTASFT--EIHEIDLSDPNAQSHIVEACEEFGFFKLVN 60
           MVVL QP + LD +  L   +KP    T   I  ++L+DP A++ IV+ACEEFGFFK+VN
Sbjct: 1   MVVLPQP-VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTANP 120
           H V  ELM +LE+EA+ F    QS K++A P +P GYG K IG NGD GWI+YLL  ANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 -------LPIFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEK 180
                    +F Q  + F  + +EY+  +K++SY+V E++ E L IEPR+ +SK+L DEK
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 SDCCFRVNHYPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVS 240
           SD C R+NHYP   +           +GFGEHTDPQIIS+LRSNN+  LQIC+KD +WV+
Sbjct: 181 SDSCLRLNHYPAAEE----EAEKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVA 240

Query: 241 VPPDAATIFVNVGDAL 248
           VPPD ++ F+NVGDAL
Sbjct: 241 VPPDHSSFFINVGDAL 251

BLAST of Lag0028958 vs. TAIR 10
Match: AT1G30040.2 (gibberellin 2-oxidase )

HSP 1 Score: 252.3 bits (643), Expect = 4.0e-67
Identity = 130/256 (50.78%), Postives = 172/256 (67.19%), Query Frame = 0

Query: 1   MVVLSQPTLGLDQYSLLRTRFKPTASFT--EIHEIDLSDPNAQSHIVEACEEFGFFKLVN 60
           MVVL QP + LD +  L   +KP    T   I  ++L+DP A++ IV+ACEEFGFFK+VN
Sbjct: 1   MVVLPQP-VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTANP 120
           H V  ELM +LE+EA+ F    QS K++A P +P GYG K IG NGD GWI+YLL  ANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 -------LPIFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEK 180
                    +F Q  + F  + +EY+  +K++SY+V E++ E L IEPR+ +SK+L DEK
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 SDCCFRVNHYPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVS 240
           SD C R+NHYP   +           +GFGEHTDPQIIS+LRSNN+  LQIC+KD +WV+
Sbjct: 181 SDSCLRLNHYPAAEE----EAEKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVA 240

Query: 241 VPPDAATIFVNVGDAL 248
           VPPD ++ F+NVGDAL
Sbjct: 241 VPPDHSSFFINVGDAL 251

BLAST of Lag0028958 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 231.5 bits (589), Expect = 7.4e-61
Identity = 114/232 (49.14%), Postives = 157/232 (67.67%), Query Frame = 0

Query: 23  PTASFTEIHEIDLSDPNAQSHIVEACEEFGFFKLVNHRVPVELMAKLEEEALCFIKHSQS 82
           P + F+ I  ID+SDP ++  +V+ACE+FGFFK++NH V  EL++ LE E + F    +S
Sbjct: 11  PKSGFSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKS 70

Query: 83  EKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTAN------PL-PIFTQNSEYFLAAAKE 142
           EK +     P GYG   IG NGD GW++YLL  AN      PL P   ++   F  A +E
Sbjct: 71  EKTQV-AGYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEE 130

Query: 143 YVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSDCCFRVNHYPPCPDLQAVGGSGR 202
           Y T+V+K++++V E I +GL I+PRN +SKL++D+ +D   R+NHYPPCP        G+
Sbjct: 131 YTTSVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGK 190

Query: 203 NMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVPPDAATIFVNVGDAL 248
           N+IGFGEHTDPQIIS+LRSNN++ LQI L D +W+SVPPD  + F NVGD+L
Sbjct: 191 NVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSL 241

BLAST of Lag0028958 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 219.5 bits (558), Expect = 2.9e-57
Identity = 114/254 (44.88%), Postives = 157/254 (61.81%), Query Frame = 0

Query: 1   MVVLSQPTLGLDQYSLLRTRFKPTASFTEIHEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MV++ QP    D    +  + KP      +  IDL+D +A++ IV+ACEEFGFFK++NH 
Sbjct: 1   MVIVLQPA-SFDSNLYVNPKCKPRPVLIPV--IDLTDSDAKTQIVKACEEFGFFKVINHG 60

Query: 61  VPVELMAKLEEEALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTAN--- 120
           V  +L+ +LE+EA+ F     S KDKA P DP GYG K IG NGD GW++Y+L  AN   
Sbjct: 61  VRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCL 120

Query: 121 ----PLPIFTQNSEYFLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSD 180
                  IF      F  A +EY+  +K++S +  E++ E LKIEP+  +S+L+  ++SD
Sbjct: 121 ESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESD 180

Query: 181 CCFRVNHYPPCPDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVP 240
            C R+NHYP   +        +  IGFGEHTDPQ+ISLLRSN++  LQIC+KD  WV V 
Sbjct: 181 SCLRMNHYPEKEETPV-----KEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVT 240

Query: 241 PDAATIFVNVGDAL 248
           PD ++ FV VGD L
Sbjct: 241 PDHSSFFVLVGDTL 246

BLAST of Lag0028958 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 183.0 bits (463), Expect = 3.0e-46
Identity = 99/243 (40.74%), Postives = 145/243 (59.67%), Query Frame = 0

Query: 17  LRTRFKPTASFTE----IHEIDLSDPNAQSH-IVEACEEFGFFKLVNHRVPVELMAKLEE 76
           L+T  K T S  E    + +  L+D +  S  IV+ACE  GFFK++NH V  E++ + E 
Sbjct: 9   LQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEH 68

Query: 77  EALCFIKHSQSEKDKARPSDPLGYGCKNIGSNGDKGWIKYLLPTANPLPIFTQNSEY--- 136
           E   F    +S+K +A P+ P GYGCKNIG NGD G ++YLL  ANP  +  ++      
Sbjct: 69  EGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHD 128

Query: 137 ----FLAAAKEYVTAVKKLSYEVAELIVEGLKIEPRNAISKLLNDEKSDCCFRVNHYPPC 196
               F +A  +Y+  V+ L+ E+ +L +E L  +  + +S+L+ D +SD   R+NHYPP 
Sbjct: 129 DPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPA 188

Query: 197 PDLQAVGGSGRNMIGFGEHTDPQIISLLRSNNSTDLQICLKDSAWVSVPPDAATIFVNVG 248
           P   A+ G G+  IGFGEH+DPQI+++LRSN+   L+IC +D  W+ +P D    FV VG
Sbjct: 189 P--YALSGVGQ--IGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVG 247

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AUB30295.12.8e-12691.50gibberellin 2-beta-dioxygenase 8-like protein [Luffa aegyptiaca][more]
XP_038883200.14.7e-11080.16gibberellin 2-beta-dioxygenase-like [Benincasa hispida][more]
XP_022977872.12.2e-10778.54gibberellin 2-beta-dioxygenase [Cucurbita maxima][more]
CBL59329.12.2e-10778.54Gibberellin 2-oxidase [Cucurbita maxima][more]
XP_008439633.11.4e-10679.35PREDICTED: gibberellin 2-beta-dioxygenase-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9XG832.8e-8159.84Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=... [more]
Q9SQ803.6e-6850.00Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1[more]
Q9XFR95.7e-6650.78Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ... [more]
Q8LEA21.0e-5949.14Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 ... [more]
O646924.1e-5644.88Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A2H4V3T71.3e-12691.50Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 S... [more]
A0A6J1IJN11.1e-10778.54gibberellin 2-beta-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111478033 PE=3 ... [more]
I0J1J01.1e-10778.54Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox2 PE=2 SV=1[more]
A0A1S3AZ816.9e-10779.35gibberellin 2-beta-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103484366 PE=3... [more]
A0A5A7UGB19.0e-10779.35Gibberellin 2-beta-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
Match NameE-valueIdentityDescription
AT1G30040.14.0e-6750.78gibberellin 2-oxidase [more]
AT1G30040.24.0e-6750.78gibberellin 2-oxidase [more]
AT1G78440.17.4e-6149.14Arabidopsis thaliana gibberellin 2-oxidase 1 [more]
AT2G34555.12.9e-5744.88gibberellin 2-oxidase 3 [more]
AT1G02400.13.0e-4640.74gibberellin 2-oxidase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 173..247
e-value: 3.5E-12
score: 46.7
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 37..102
e-value: 4.9E-10
score: 40.2
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 13..247
e-value: 7.9E-45
score: 155.5
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 9..247
NoneNo IPR availablePANTHERPTHR47990:SF121GIBBERELLIN 2-BETA-DIOXYGENASE 2coord: 9..247
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 21..247

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0028958.1Lag0028958.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding