Lag0028935 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0028935 vs. NCBI nr
Match: XP_022989992.1 (putative calcium-binding protein CML19 [Cucurbita maxima])

HSP 1 Score: 222.2 bits (565), Expect = 2.7e-54
Identity = 113/140 (80.71%), Postives = 129/140 (92.14%), Query Frame = 0

Query: 1   MDKFSRYESVFNTIDADCDGKVSPQDLLSRFSAVTGEELTEAEAAAAVAAFDSDGDGLLE 60
           M +FSRY+ VFN+ID+DCDGKVS QDLL+RF+AVTGE LTE EAAA VAA+DSD DGLLE
Sbjct: 1   MPQFSRYQRVFNSIDSDCDGKVSSQDLLTRFAAVTGEHLTEEEAAAVVAAYDSDDDGLLE 60

Query: 61  MAEFERFV-EGDEEGREMREAFEMYEMEGLGVITAVSLRRMLSRLGESRTVADCKRMIAK 120
           +AEFERFV EGD EG+E++EAF+MYEMEGLGVITAVSLRRMLS+LGESR++ DC RMIAK
Sbjct: 61  IAEFERFVEEGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGESRSIGDCNRMIAK 120

Query: 121 FDVNGDGVLCFDEFRVMMLL 140
           FDVNGDGVLCFDEFR+MMLL
Sbjct: 121 FDVNGDGVLCFDEFRIMMLL 140

BLAST of Lag0028935 vs. NCBI nr
Match: XP_022962715.1 (probable calcium-binding protein CML31 [Cucurbita moschata] >KAG6601838.1 putative calcium-binding protein CML31, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032546.1 putative calcium-binding protein CML31, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 216.1 bits (549), Expect = 1.9e-52
Identity = 111/140 (79.29%), Postives = 128/140 (91.43%), Query Frame = 0

Query: 1   MDKFSRYESVFNTIDADCDGKVSPQDLLSRFSAVTGEELTEAEAAAAVAAFDSDGDGLLE 60
           M +FSRY+ VFN+ID+D DGKVS QDLL+RF+AVTGEELTE EAAA VAA+DSD DGLLE
Sbjct: 1   MPQFSRYQRVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLE 60

Query: 61  MAEFERFVE-GDEEGREMREAFEMYEMEGLGVITAVSLRRMLSRLGESRTVADCKRMIAK 120
           +AEFER VE GD EG+E++EAF+MYEMEGLGVITAVSLRRMLS+LG+SR++ DC RMIAK
Sbjct: 61  IAEFERLVEDGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLSQLGQSRSIGDCNRMIAK 120

Query: 121 FDVNGDGVLCFDEFRVMMLL 140
           FDVNGDGVLCFDEFR+MMLL
Sbjct: 121 FDVNGDGVLCFDEFRIMMLL 140

BLAST of Lag0028935 vs. NCBI nr
Match: XP_023520938.1 (probable calcium-binding protein CML31 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 213.4 bits (542), Expect = 1.3e-51
Identity = 110/137 (80.29%), Postives = 125/137 (91.24%), Query Frame = 0

Query: 1   MDKFSRYESVFNTIDADCDGKVSPQDLLSRFSAVTGEELTEAEAAAAVAAFDSDGDGLLE 60
           M +FSRYE VFN+ID+D DGKVS QDLL+RF+AVTGEELTE EAAA VAA+DSD DGLLE
Sbjct: 1   MPQFSRYERVFNSIDSDGDGKVSSQDLLTRFAAVTGEELTEEEAAAVVAAYDSDDDGLLE 60

Query: 61  MAEFERFV-EGDEEGREMREAFEMYEMEGLGVITAVSLRRMLSRLGESRTVADCKRMIAK 120
           +AEFERFV EGD EG+E++EAF+MYEMEGLGVITAVSLRRML +LGESR++ DC RMIAK
Sbjct: 61  IAEFERFVEEGDGEGKEIKEAFKMYEMEGLGVITAVSLRRMLIQLGESRSIGDCNRMIAK 120

Query: 121 FDVNGDGVLCFDEFRVM 137
           FDVNGDGVLCFDEFR+M
Sbjct: 121 FDVNGDGVLCFDEFRIM 137

BLAST of Lag0028935 vs. NCBI nr
Match: XP_038875509.1 (putative calcium-binding protein CML23 [Benincasa hispida])

HSP 1 Score: 202.6 bits (514), Expect = 2.2e-48
Identity = 107/139 (76.98%), Postives = 120/139 (86.33%), Query Frame = 0

Query: 1   MDKFSRYESVFNTIDADCDGKVSPQDLLSRFSAVTGEELTEAEAAAAVAAFDSDGDGLLE 60
           MDKF+    +FNTID DCDGKVSP DLL+R   +TGE+LTE +AAAAVAAFD +GDGLL+
Sbjct: 1   MDKFT---LLFNTIDTDCDGKVSPHDLLTRLPPLTGEDLTEVDAAAAVAAFDWNGDGLLD 60

Query: 61  MAEFERFVEGDEEGREMREAFEMYEMEGLGVITAVSLRRMLSRLGESRTVADCKRMIAKF 120
           M EF R VEGD+E  E+REAF+MYEMEGLGVIT VSLRRMLSRLGESR+V DCK+MIAKF
Sbjct: 61  MEEFGRLVEGDDE-EEVREAFQMYEMEGLGVITPVSLRRMLSRLGESRSVGDCKKMIAKF 120

Query: 121 DVNGDGVLCFDEFRVMMLL 140
           DVNGDGVLCFDEFR MMLL
Sbjct: 121 DVNGDGVLCFDEFRTMMLL 135

BLAST of Lag0028935 vs. NCBI nr
Match: XP_004150479.1 (probable calcium-binding protein CML31 [Cucumis sativus] >KGN51513.1 hypothetical protein Csa_008833 [Cucumis sativus])

HSP 1 Score: 196.4 bits (498), Expect = 1.6e-46
Identity = 107/145 (73.79%), Postives = 120/145 (82.76%), Query Frame = 0

Query: 1   MDKFSRYES-VFNTIDADCDGKVSPQDLLSRFSAVTGEELTEAEAAAAVAAFDSDGDGLL 60
           MDK S Y + +F  ID D DGKVSP DLL+R  A+TG+ LTEAEAA+AVAAFDSDGDGLL
Sbjct: 1   MDKLSPYHTLLFYNIDTDSDGKVSPHDLLTRLPALTGQVLTEAEAASAVAAFDSDGDGLL 60

Query: 61  EMAEFERFVE-----GDEEGREMREAFEMYEMEGLGVITAVSLRRMLSRLGESRTVADCK 120
            M EF R VE     GD++ RE+REAF+MYEMEGLGVIT VSLRRMLSRLGESR+V DCK
Sbjct: 61  GMEEFGRLVEGVVVGGDDDDREVREAFQMYEMEGLGVITPVSLRRMLSRLGESRSVGDCK 120

Query: 121 RMIAKFDVNGDGVLCFDEFRVMMLL 140
           RMIAKFD++GDGVLCFDEFR MMLL
Sbjct: 121 RMIAKFDLDGDGVLCFDEFRGMMLL 145

BLAST of Lag0028935 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 5.9e-28
Identity = 69/136 (50.74%), Postives = 88/136 (64.71%), Query Frame = 0

Query: 9   SVFNTIDADCDGKVSPQDLLSRFSAVTGEELTEAEAAAAVAAFDSDGDGLLEMAEFERFV 68
           ++F T D D DG++S  +L        GEE+++ EA   VA+ D+DGDGLL  AEF R V
Sbjct: 14  ALFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVRLV 73

Query: 69  -------EGDEEGREMREAFEMYEMEGLGVITAVSLRRMLSRLGESRTVADCKRMIAKFD 128
                  E +  G  +REAF MYEMEG G IT  SLRRML RLG  + + DC+ MI +FD
Sbjct: 74  QAAEVEEEDERRGTGLREAFGMYEMEGEGCITPTSLRRMLRRLGSDQDIDDCRAMICRFD 133

Query: 129 VNGDGVLCFDEFRVMM 138
           +NGDGVL FDEF++MM
Sbjct: 134 LNGDGVLSFDEFKIMM 149

BLAST of Lag0028935 vs. ExPASy Swiss-Prot
Match: Q8RYJ9 (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.5e-26
Identity = 73/149 (48.99%), Postives = 94/149 (63.09%), Query Frame = 0

Query: 2   DKFSRYESVFNTIDADCDGKVSPQDLLSRFSAVTGEELTEAEAAAAVAAFDSDGDGLLEM 61
           D+F R   VF + D D DGK+S  +L     A  GE++ + E  A +A  D+DGDGLL+ 
Sbjct: 5   DEFRR---VFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDE 64

Query: 62  AEFERFV-----EGDEEG-------REMREAFEMYEMEGLGVITAVSLRRMLSRLGESRT 121
            EF R V     +GDEE        R +REAF MYEMEG G IT +SL+ MLS+LG    
Sbjct: 65  EEFVRLVTEMEADGDEEEDDDDETCRCLREAFAMYEMEGRGCITPLSLKLMLSKLGTHLD 124

Query: 122 VADCKRMIAKFDVNGDGVLCFDEFRVMML 139
           VA+C+ MI +FD+NGDGVL FDEF+ MM+
Sbjct: 125 VAECQAMICRFDMNGDGVLTFDEFKTMMM 150

BLAST of Lag0028935 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.5e-26
Identity = 66/139 (47.48%), Postives = 94/139 (67.63%), Query Frame = 0

Query: 2   DKFSRYESVFNTIDADCDGKVSPQDLLSRFSAVTGEELTEAEAAAAVAAFDSDGDGLLEM 61
           DK    E+VF+ +DA+ DG++SP++L   F  + GE+L++ EA AAV   D+DGDG+L+ 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTL-GEQLSDEEAVAAVRLSDTDGDGMLDF 98

Query: 62  AEFERFVEGDEEGR---EMREAFEMYEMEGLGVITAVSLRRMLSRLGESRTVADCKRMIA 121
            EF + ++ D+E     E++ AF +Y  EG   IT  SL+ ML +LGESRT  DC+ MI+
Sbjct: 99  EEFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMIS 158

Query: 122 KFDVNGDGVLCFDEFRVMM 138
            FD+N DGVL FDEF +MM
Sbjct: 159 AFDLNADGVLSFDEFALMM 176

BLAST of Lag0028935 vs. ExPASy Swiss-Prot
Match: Q2QYW1 (Probable calcium-binding protein CML25/26 OS=Oryza sativa subsp. japonica OX=39947 GN=CML25 PE=3 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.1e-25
Identity = 74/146 (50.68%), Postives = 90/146 (61.64%), Query Frame = 0

Query: 5   SRYESVFNTIDADCDGKVSPQDLLSRFSAVTGEELTEAEAAAAVAAFDSDGDGLLEMAEF 64
           S   SVF   D D DGKVS  +L    +A  GEE++E EAAA +A  D+DGDGLL+  EF
Sbjct: 3   SSASSVFAAFDKDGDGKVSASELRGCMAAALGEEVSEEEAAAILATADTDGDGLLDHHEF 62

Query: 65  ERF-------VEGDEEGREMREAFEMY-EMEGLGVITAVSLRRMLSRLGESR---TVADC 124
            R           +E  R +REAF+MY E E   VIT  SLRRML RLG       + +C
Sbjct: 63  MRLSAAHQLQEPAEESLRCLREAFDMYAEEEETAVITPASLRRMLRRLGSEHQRLEMEEC 122

Query: 125 KRMIAKFDVNGDGVLCFDEFRVMMLL 140
           + MI +FD+NGDGVL FDEFRVMML+
Sbjct: 123 RAMICRFDLNGDGVLSFDEFRVMMLM 148

BLAST of Lag0028935 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 3.6e-25
Identity = 63/139 (45.32%), Postives = 90/139 (64.75%), Query Frame = 0

Query: 5   SRYESVFNTIDADCDGKVSPQDLLSRFSAVTGEELTEAEAAAAVAAFDSDGDGLLEMAEF 64
           + +  VF+  D D DGK+S  +L     A  GE+++  EA A V++ D+D DGLL+  EF
Sbjct: 6   AEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEF 65

Query: 65  ERFV----EGDEEG--REMREAFEMYEMEGLGVITAVSLRRMLSRLGESRTVADCKRMIA 124
            +       GDEE   R + EAF MYEMEG G IT  SL+RMLS+LG  + + +C+ MI 
Sbjct: 66  TKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQTMIC 125

Query: 125 KFDVNGDGVLCFDEFRVMM 138
           +FD++GDGV+ F+EF++MM
Sbjct: 126 RFDLDGDGVISFEEFKIMM 144