Lag0028927 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0028927
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr8: 33009337 .. 33010912 (-)
RNA-Seq ExpressionLag0028927
SyntenyLag0028927
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTACCTCGATGAAATTTGTCCAATATGTGCAGGATGTGGACGCACTAGAGGCAATGGCGATCCGCGACAGTCTGATTGTTGCTAAAGAAGCCGGCTTCTTGAGACTAGAGGTGGAGACTGATTCAGCTCGTGTGGCAGCCATTATTCGATCAAGCCATAAGGATTGCTCTGAGGTGGGAGTTCTGGTACAGGAGATCAATCAGATCTTGAAGGAGTTTCTGTTCTGCTCTGTGCGTTGGTGCCGGAGGGAGGCTAATCTGTTGGCTCATAGGTGGCGCGTCAGGTGCTGGAGCGTGGCATCCAGGGCATTTGGTTAGAAGAAGTTCTGGTGACCCTGGGAGAGGTTTATCGCAGTGAGCTTTTGGACAGTGAAGGCGAGAGATCGAGATCCTTATCTGAGGGGTGTTGTTAGTTTTCTTTGTGTATCCTTCTTTTATAAAAAAAATATAATAATAATCCAAAAACTAAATATCATATAATTATCAAAAAAGACCTCTAAGTGATTTCTTTTTAAATGTGGGGTCAATGGTTCGAATCTTGATTTAAAAGGAAGCAGTGGATATTTAATCGAATGAAAAATAATTGGGAGAGATATTTTATCGAATGGAAACCCTAATTCAATGATAAATAGAAGAGGCTAACCCCAAGGTGAGGGGATTCCAACGAGGTATTTTTTTTCTTTCTTAATTTACTTGCTTCACGGATGATCTCCCAACTGACTTAAGCATCGGAGTTGGTGTGACAAGCATCACACCGGTGCACAATTTTCTCGTGTTTTTCAGGCCGCCACGTCTTTCCCCTATAAACAAATTTACCATTGTTGTCACGTGAAGGTTCATGTGAGTTTCCCTGCCCGAATTTTGGCATCAACAGCACCTATCGATTCGTCATTGGTAATTGTTAATTGGTCAGTCCCCAAATTAACATTGTCTAGATCTGCCCTTGCTCAAAGAGTCCATTTTTTAAACTTCAACAACTACACCGATACCAAAATCAAATTTTAAAGAACAAAAAGTGTATTTTACTCTATTTTATTTTTCACATTTTGACAAAAGTGGGTGTTGTGGGTAATTTTGGCTTGGCAGGTACACCAAAGAAGAAGGGCTTCGGACCCAGCTGAGATCGGCCCGAGGAGATGAAGGCCCGCAAGAGGTGGAATGTCACCTTGGCCTCGGCCCAATGCCGAGGCCGAGGGTCTGGTTGGCCTCGGCCTAATGCCGAGGCCGACCAGGCCAATTAAGCCCACTGGCCACTTGGGTTGCCCGGGTCAATCTCCTTGGGCCCAGTAGCAGGAGGCTACGTGCGGTGGACGACGCGGAAAGGGCAAAACCCTAGCGAGAGAGGTATAAAAGGCTAGAGATGATCCAGAAAAAGGGGTAACTGATATCTGAACTCTAAACTCCATTGAACTAGGTTTTCTGGACTGGGAGACCGGCCGGAGATTTTCCGATCACTGACTTAGGCATCGGAGCACGTGTGGCGAGCACCACACCGGTGTGCAGTGCTCACTTTCTTTCTTTGCAGGTCACGTCTTCTCCATACCTCAACAAGTTCATTGTTGGTGTCACGTGA

mRNA sequence

ATGCTTACCTCGATGAAATTTGTCCAATATGTGCAGGATGTGGACGCACTAGAGGCAATGGCGATCCGCGACAGTCTGATTGTTGCTAAAGAAGCCGGCTTCTTGAGACTAGAGGTGGAGACTGATTCAGCTCGTGTGGCAGCCATTATTCGATCAAGCCATAAGGATTGCTCTGAGGTGGGAGTTCTGGTACAGGAGATCAATCAGATCTTGAAGGAGTTTCTGTTCTGCTCTGTGCGTTGGTGCCGGAGGGAGGCTAATCTGTTGGCTCATAGGTGGCGCGTCAGGTACACCAAAGAAGAAGGGCTTCGGACCCAGCTGAGATCGGCCCGAGGAGATGAAGGCCCGCAAGAGGTGGAATGTCACCTTGGCCTCGGCCCAATGCCGAGGCCGAGGGTTTTCTGGACTGGGAGACCGGCCGGAGATTTTCCGATCACTGACTTAGGCATCGGAGCACGTGTGGCGAGCACCACACCGGTGTGCAGTGCTCACTTTCTTTCTTTGCAGGTCACGTCTTCTCCATACCTCAACAAGTTCATTGTTGGTGTCACGTGA

Coding sequence (CDS)

ATGCTTACCTCGATGAAATTTGTCCAATATGTGCAGGATGTGGACGCACTAGAGGCAATGGCGATCCGCGACAGTCTGATTGTTGCTAAAGAAGCCGGCTTCTTGAGACTAGAGGTGGAGACTGATTCAGCTCGTGTGGCAGCCATTATTCGATCAAGCCATAAGGATTGCTCTGAGGTGGGAGTTCTGGTACAGGAGATCAATCAGATCTTGAAGGAGTTTCTGTTCTGCTCTGTGCGTTGGTGCCGGAGGGAGGCTAATCTGTTGGCTCATAGGTGGCGCGTCAGGTACACCAAAGAAGAAGGGCTTCGGACCCAGCTGAGATCGGCCCGAGGAGATGAAGGCCCGCAAGAGGTGGAATGTCACCTTGGCCTCGGCCCAATGCCGAGGCCGAGGGTTTTCTGGACTGGGAGACCGGCCGGAGATTTTCCGATCACTGACTTAGGCATCGGAGCACGTGTGGCGAGCACCACACCGGTGTGCAGTGCTCACTTTCTTTCTTTGCAGGTCACGTCTTCTCCATACCTCAACAAGTTCATTGTTGGTGTCACGTGA

Protein sequence

MLTSMKFVQYVQDVDALEAMAIRDSLIVAKEAGFLRLEVETDSARVAAIIRSSHKDCSEVGVLVQEINQILKEFLFCSVRWCRREANLLAHRWRVRYTKEEGLRTQLRSARGDEGPQEVECHLGLGPMPRPRVFWTGRPAGDFPITDLGIGARVASTTPVCSAHFLSLQVTSSPYLNKFIVGVT
Homology
[Close]
BLAST of Lag0028927 vs. NCBI nr
Match: KAF7819670.1 (uncharacterized protein G2W53_025125 [Senna tora])

HSP 1 Score: 65.9 bits (159), Expect = 4.3e-07
Identity = 33/91 (36.26%), Postives = 54/91 (59.34%), Query Frame = 0 

Query: 1   MLTSMKFVQYVQDVDALEAMAIRDSLIVAKEAGFLRLEVETDSARVAAIIRSSHKDCSEV 60
           ++   K  +    VD LEA+A+ + L++AK    L +EVE DSA+V  ++     D S +
Sbjct: 121 LVAMTKRFEVATTVDTLEALAVYEGLMLAKSMSILNIEVEGDSAKVFKLLNKKGTDQSYL 180

Query: 61  GVLVQEINQILKEFLFCSVRWCRREANLLAH 92
           G+++++I ++   F F S +W RRE N LAH
Sbjct: 181 GIIIEDILELSSFFNFVSFKWIRRETNKLAH 211

[Close]
BLAST of Lag0028927 vs. NCBI nr
Match: KAF7835887.1 (uncharacterized protein G2W53_010746 [Senna tora])

HSP 1 Score: 61.6 bits (148), Expect = 8.1e-06
Identity = 30/81 (37.04%), Postives = 48/81 (59.26%), Query Frame = 0 

Query: 11  VQDVDALEAMAIRDSLIVAKEAGFLRLEVETDSARVAAIIRSSHKDCSEVGVLVQEINQI 70
           +  VD +EA+A+ D L++AK    L +EVE DSA V  ++     D S +G+++++I  +
Sbjct: 80  ITSVDTMEAVAVYDGLVLAKSMSILNIEVEGDSANVFKLLNRGGVDQSYLGIIIEDILDL 139

Query: 71  LKEFLFCSVRWCRREANLLAH 92
              F   S +W RRE N +AH
Sbjct: 140 SNFFSSISFKWIRRETNRVAH 160

[Close]
BLAST of Lag0028927 vs. NCBI nr
Match: XP_024317835.1 (uncharacterized protein LOC112271862 [Brachypodium distachyon] >XP_024317836.1 uncharacterized protein LOC112271862 [Brachypodium distachyon] >PNT68773.1 hypothetical protein BRADI_3g45155v3 [Brachypodium distachyon] >PNT68774.1 hypothetical protein BRADI_3g45155v3 [Brachypodium distachyon])

HSP 1 Score: 60.1 bits (144), Expect = 2.4e-05
Identity = 32/91 (35.16%), Postives = 53/91 (58.24%), Query Frame = 0 

Query: 1   MLTSMKFVQYVQDVDALEAMAIRDSLIVAKEAGFLRLEVETDSARVAAIIRSSHKDCSEV 60
           M+   +  Q + D   +EA+A+RD+  +AKE  F R+ +ETD A +  + R+     S V
Sbjct: 208 MVARSRVYQGISDPLIVEALALRDACCLAKEKAFQRVILETDCAELVRLWRARSSQRSVV 267

Query: 61  GVLVQEINQILKEFLFCSVRWCRREANLLAH 92
           G ++ EI +  + F+ C + + RREAN +AH
Sbjct: 268 GPILDEIIEFSQSFISCEIVFVRREANFVAH 298

[Close]
BLAST of Lag0028927 vs. NCBI nr
Match: XP_030939479.1 (uncharacterized protein LOC115964277 [Quercus lobata])

HSP 1 Score: 59.7 bits (143), Expect = 3.1e-05
Identity = 30/79 (37.97%), Postives = 47/79 (59.49%), Query Frame = 0 

Query: 14   VDALEAMAIRDSLIVAKEAGFLRLEVETDSARVAAIIRSSHKDCSEVGVLVQEINQILKE 73
            V+ +E +A R +L+ A+E GF R+ +E DS  V   I   + DCS +G ++Q+I  +   
Sbjct: 1136 VEMVEVLAARQALVFARELGFDRMILEGDSETVIKAILGDYMDCSYMGYVLQDIKLLFSS 1195

Query: 74   FLFCSVRWCRREANLLAHR 93
            F F SV+   RE N +AH+
Sbjct: 1196 FSFISVKHIHREGNCVAHK 1214

[Close]
BLAST of Lag0028927 vs. NCBI nr
Match: KAF7802400.1 (uncharacterized protein G2W53_041511 [Senna tora])

HSP 1 Score: 58.9 bits (141), Expect = 5.2e-05
Identity = 29/78 (37.18%), Postives = 45/78 (57.69%), Query Frame = 0 

Query: 14  VDALEAMAIRDSLIVAKEAGFLRLEVETDSARVAAIIRSSHKDCSEVGVLVQEINQILKE 73
           V+  EA+A+ + ++VAK    L + VE DSA V  ++     DC+ V  ++++I Q+   
Sbjct: 654 VEMAEALAVYEGMVVAKRMSILNVLVEGDSASVIKLLNGEGMDCTYVSFIIKDILQLCSS 713

Query: 74  FLFCSVRWCRREANLLAH 92
           F   S  W RREAN +AH
Sbjct: 714 FRSYSFNWVRREANKVAH 731

The following BLAST results are available for this feature:
BLAST of Lag0028927 vs. NCBI nr
Match NameE-valueIdentityDescription
KAF7819670.14.3e-0736.26uncharacterized protein G2W53_025125 [Senna tora][more]
KAF7835887.18.1e-0637.04uncharacterized protein G2W53_010746 [Senna tora][more]
XP_024317835.12.4e-0535.16uncharacterized protein LOC112271862 [Brachypodium distachyon] >XP_024317836.1 u... [more]
XP_030939479.13.1e-0537.97uncharacterized protein LOC115964277 [Quercus lobata][more]
KAF7802400.15.2e-0537.18uncharacterized protein G2W53_041511 [Senna tora][more]
BLAST of Lag0028927 vs. NCBI nr
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 2..101
e-value: 1.7E-6
score: 30.0
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 5..91
e-value: 2.0E-13
score: 50.2
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 18..92
e-value: 2.90602E-13
score: 61.5612

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0028927.1Lag0028927.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity