Lag0028898 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0028898
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNA-directed DNA polymerase (reverse transcriptase)-related family protein
Locationchr8: 32532222 .. 32533259 (-)
RNA-Seq ExpressionLag0028898
SyntenyLag0028898
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGATGGCGGGTTGGAAATGGCAAGCATATCAAAATTGGGGAAGATCCTTGGGTGGGAGAGGTTAATGGTTATAAGCCGATCTTGTTGAAAAAAGAGTTGGCAGGTTCCTATGTGAGTACCCTTATTGATGGGAATGGGGGCTGGAACGAAAATCTCATTAAAGAGAGTTTTATCCAAGCTGATGCAGAAGCTATTGCCAGAATCCCTTTGGGAAGGCTTGCCAATCAGGATGAAATAATCTGGGGTCCGGATCCAAAAGGAGCTTTCTCGGTGAAGAGTGCTTATCTTTTAGGCCAATCCATTATTTCAGTGGGAAGAGCTTCCTCTTCAGACCTTTCAGAATCAAAAGCAACGTGGAGGGACTTTTGGAAGGCAAAATCAAAACCTAGAGAAACAATATGTGCTTGGAGGGCTATTCAAAATTCTCTTCCCACCCTCTCAAATATTGTGTCTAGAGGAATAGATACTAACTCTCTATGTTTTCTTTGCAGGAAAAAGGAAGAATCGGGCAGCCATGTTTTTTGGGAATGTAAGTTTTCTCAGAAAATTTGGAAGTTCTTCCTCCCTAAGGTTAGTTTTCTTCTCGATGGTTTCAGGTTGGAGGTCAATGCTAGGGATCATTGGAGCAAGCTGAGGATCCAACTAAAAGAAGAGGAGAGGGACCTAGCTATTAGCATCATTTGGCATATTTGGAACCATCGAAATTCAGTCAAAATTAAAAAAGTTCCGCCAAACCACAGAGCAGTCGTTAGAGATATCGAGATTAGCTTGGAAGGTAGGAGGTACCTGAAGAATCTGCCTTCGCCTCAGCCTAGATCGGAGAGTTGCTCGAGTCAGGGGAATTGGATCCCTCCCCCTCCGGGAAGCTGGAAAATCAACTCCGATGCCTCGTGGTCGGACGCAAAGGGTATCGGAGGGATCGGTTGGATAGCCCGTGACTCCAATGGGTCTCCCATCTTCGCTGGAGTAAAGAGGATTTGCAAGAAATGGCCGAGTTGTATGTTAGTGTTATTGAGTATTCAAGTTTCTGTTTGA

mRNA sequence

ATGAGATGGCGGGTTGGAAATGGCAAGCATATCAAAATTGGGGAAGATCCTTGGGTGGGAGAGGTTAATGGTTATAAGCCGATCTTGTTGAAAAAAGAGTTGGCAGGTTCCTATGTGAGTACCCTTATTGATGGGAATGGGGGCTGGAACGAAAATCTCATTAAAGAGAGTTTTATCCAAGCTGATGCAGAAGCTATTGCCAGAATCCCTTTGGGAAGGCTTGCCAATCAGGATGAAATAATCTGGGGTCCGGATCCAAAAGGAGCTTTCTCGGTGAAGAGTGCTTATCTTTTAGGCCAATCCATTATTTCAGTGGGAAGAGCTTCCTCTTCAGACCTTTCAGAATCAAAAGCAACGTGGAGGGACTTTTGGAAGGCAAAATCAAAACCTAGAGAAACAATATGTGCTTGGAGGGCTATTCAAAATTCTCTTCCCACCCTCTCAAATATTGTGTCTAGAGGAATAGATACTAACTCTCTATGTTTTCTTTGCAGGAAAAAGGAAGAATCGGGCAGCCATGTTTTTTGGGAATGTAAGTTTTCTCAGAAAATTTGGAAGTTCTTCCTCCCTAAGGTTAGTTTTCTTCTCGATGGTTTCAGGTTGGAGGTCAATGCTAGGGATCATTGGAGCAAGCTGAGGATCCAACTAAAAGAAGAGGAGAGGGACCTAGCTATTAGCATCATTTGGCATATTTGGAACCATCGAAATTCAGTCAAAATTAAAAAAGTTCCGCCAAACCACAGAGCAGTCGTTAGAGATATCGAGATTAGCTTGGAAGGTAGGAGGTACCTGAAGAATCTGCCTTCGCCTCAGCCTAGATCGGAGAGTTGCTCGAGTCAGGGGAATTGGATCCCTCCCCCTCCGGGAAGCTGGAAAATCAACTCCGATGCCTCGTGGTCGGACGCAAAGGGTATCGGAGGGATCGGTTGGATAGCCCGTGACTCCAATGGGTCTCCCATCTTCGCTGGAGTAAAGAGGATTTGCAAGAAATGGCCGAGTTGTATGTTAGTGTTATTGAGTATTCAAGTTTCTGTTTGA

Coding sequence (CDS)

ATGAGATGGCGGGTTGGAAATGGCAAGCATATCAAAATTGGGGAAGATCCTTGGGTGGGAGAGGTTAATGGTTATAAGCCGATCTTGTTGAAAAAAGAGTTGGCAGGTTCCTATGTGAGTACCCTTATTGATGGGAATGGGGGCTGGAACGAAAATCTCATTAAAGAGAGTTTTATCCAAGCTGATGCAGAAGCTATTGCCAGAATCCCTTTGGGAAGGCTTGCCAATCAGGATGAAATAATCTGGGGTCCGGATCCAAAAGGAGCTTTCTCGGTGAAGAGTGCTTATCTTTTAGGCCAATCCATTATTTCAGTGGGAAGAGCTTCCTCTTCAGACCTTTCAGAATCAAAAGCAACGTGGAGGGACTTTTGGAAGGCAAAATCAAAACCTAGAGAAACAATATGTGCTTGGAGGGCTATTCAAAATTCTCTTCCCACCCTCTCAAATATTGTGTCTAGAGGAATAGATACTAACTCTCTATGTTTTCTTTGCAGGAAAAAGGAAGAATCGGGCAGCCATGTTTTTTGGGAATGTAAGTTTTCTCAGAAAATTTGGAAGTTCTTCCTCCCTAAGGTTAGTTTTCTTCTCGATGGTTTCAGGTTGGAGGTCAATGCTAGGGATCATTGGAGCAAGCTGAGGATCCAACTAAAAGAAGAGGAGAGGGACCTAGCTATTAGCATCATTTGGCATATTTGGAACCATCGAAATTCAGTCAAAATTAAAAAAGTTCCGCCAAACCACAGAGCAGTCGTTAGAGATATCGAGATTAGCTTGGAAGGTAGGAGGTACCTGAAGAATCTGCCTTCGCCTCAGCCTAGATCGGAGAGTTGCTCGAGTCAGGGGAATTGGATCCCTCCCCCTCCGGGAAGCTGGAAAATCAACTCCGATGCCTCGTGGTCGGACGCAAAGGGTATCGGAGGGATCGGTTGGATAGCCCGTGACTCCAATGGGTCTCCCATCTTCGCTGGAGTAAAGAGGATTTGCAAGAAATGGCCGAGTTGTATGTTAGTGTTATTGAGTATTCAAGTTTCTGTTTGA

Protein sequence

MRWRVGNGKHIKIGEDPWVGEVNGYKPILLKKELAGSYVSTLIDGNGGWNENLIKESFIQADAEAIARIPLGRLANQDEIIWGPDPKGAFSVKSAYLLGQSIISVGRASSSDLSESKATWRDFWKAKSKPRETICAWRAIQNSLPTLSNIVSRGIDTNSLCFLCRKKEESGSHVFWECKFSQKIWKFFLPKVSFLLDGFRLEVNARDHWSKLRIQLKEEERDLAISIIWHIWNHRNSVKIKKVPPNHRAVVRDIEISLEGRRYLKNLPSPQPRSESCSSQGNWIPPPPGSWKINSDASWSDAKGIGGIGWIARDSNGSPIFAGVKRICKKWPSCMLVLLSIQVSV
Homology
BLAST of Lag0028898 vs. NCBI nr
Match: EEE69144.1 (hypothetical protein OsJ_28268 [Oryza sativa Japonica Group])

HSP 1 Score: 178.3 bits (451), Expect = 1.1e-40
Identity = 108/343 (31.49%), Postives = 164/343 (47.81%), Query Frame = 0

Query: 3    WRVGNGKHIKIGEDPWVGEVNGYKPILLKKELAGSYVSTLIDGNGGWNENLIKESFIQAD 62
            W +GNGK +++ +DPW+      +PI  K+     +VS LID +G W+ + I   F+  D
Sbjct: 800  WHIGNGKSVRLWKDPWIPRSYSRRPISAKRNCRLRWVSDLIDQSGSWDNDKISHHFLPMD 859

Query: 63   AEAIARIPLGRLANQDEIIWGPDPKGAFSVKSAYLLGQSIISVGRASSSDLSESKATWRD 122
            AEAI  I L     +D I W PD  G FSV+SAY L  ++  V   SSS  + S      
Sbjct: 860  AEAILNIHLSSRLEEDFIAWHPDRLGRFSVRSAYHLAVALAQVNDGSSSSGNGSSRACNA 919

Query: 123  FWKAKSKPRETICAWRAIQNSLPTLSNIVSRGIDTNSLCFLCRKKEESGSHVFWECKFSQ 182
             WK  +  +  I AWRAI NSL TL N   R ++ + +C +C  + E   H  + C  ++
Sbjct: 920  LWKCNAPQKVKIFAWRAITNSLTTLENKKKRKLEVSDICTICGVESEYVVHALFHCPHAR 979

Query: 183  KIWKFFLPKVSFLLDGFRLE--VNARDHWSKLRIQLKE----EERDLAISIIWHIWNHRN 242
            ++W+        + D  +L    N  +  SK+ + L E    E++ + + ++W IW+ RN
Sbjct: 980  QLWE-------AMSDDMQLNPLSNIHNGDSKVILDLLEQSQPEDQVMLLMVLWRIWHTRN 1039

Query: 243  SVKIKKVPPNHRAVVRDIEISLEGRRYLKNLPSPQP------------RSESCS-----S 302
             +   K  P      R IE  +     +K  P   P            +S S       +
Sbjct: 1040 EIVHGKPAPGILVSKRFIESYVLSLAEIKQHPQANPEKGKHVVDVVLKKSHSIKRSREPA 1099

Query: 303  QGNWIPPPPGSWKINSDASWSDAKGIGGIGWIARDSNGSPIFA 323
               W  P PGS K+N D S+ +++G GGIG + R+  G  IFA
Sbjct: 1100 PDKWSKPLPGSMKLNVDGSFQESEGKGGIGAVLRNCTGEVIFA 1135

BLAST of Lag0028898 vs. NCBI nr
Match: XP_024038343.1 (uncharacterized protein LOC112097373 [Citrus clementina])

HSP 1 Score: 177.2 bits (448), Expect = 2.5e-40
Identity = 109/326 (33.44%), Postives = 166/326 (50.92%), Query Frame = 0

Query: 2    RWRVGNGKHIKIGEDPWVGEVNGYKPILLKKELAGSYVSTLIDGNGGWNENLIKESFIQA 61
            +WR+GNG+ I + ++ W+     +KPI      A + V+ LI+ +  WNE LI   F + 
Sbjct: 850  KWRIGNGQSIMLHKENWLPRPLTFKPISKPSLPADALVAELINEDHTWNEGLIHRHFTKI 909

Query: 62   DAEAIARIPLGRLANQDEIIWGPDPKGAFSVKSAYLLGQSIISVGRASSSDLSESKATWR 121
            DAEAI RI L R   +DE+IW  D KG +SVKS Y +  ++    + +SS  +E++  W 
Sbjct: 910  DAEAIVRILLPRRPMKDEVIWHYDKKGQYSVKSGYQIALNLKFPAKPTSSATTENQ--WN 969

Query: 122  DFWKAKSKPRETICAWRAIQNSLPTLSNIVSRGIDTNSLCFLCRKKEESGSHVFWECKFS 181
              W      +  I AWRA +N LP+  N+  R +    LC  C KK E+  H    CK  
Sbjct: 970  AIWLLTLPEKIRIFAWRAAKNLLPSAENLWKRKVLQEPLCQFCNKKLETIFHALVGCKVV 1029

Query: 182  QKIWKFFLPKVSFLLDGFR--LEVNARDHWSKLRIQLKEEERDLAISIIWHIWNHRNSVK 241
            QKIW     K++   D  +  +E +      +L+++  +++ +L ++I+W IWN RN+  
Sbjct: 1030 QKIW-----KITRFEDDLKDSVEQDMLSLLIELKLRRSKDDIELLVTILWMIWNARNNWI 1089

Query: 242  IKKVPPNHRAVVRDIEISLEGRRYLKNLPSPQPRSESCSSQGNWIPPPPGSWKINSDASW 301
             K V       V   E  LE  R  +   +     +S S    W PP  G +K+N DA+ 
Sbjct: 1090 FKGVNDIPHVTVSKAEAVLEAFRRTQLPAATHIDIQSSSKLKAWKPPQRGYFKVNVDAAT 1149

Query: 302  SDAKGIGGIGWIARDSNGSPIFAGVK 326
            +  K I G+G + RD  G+ I A VK
Sbjct: 1150 NLEKQIAGLGAVIRDEAGNVIAAAVK 1168

BLAST of Lag0028898 vs. NCBI nr
Match: EEC83100.1 (hypothetical protein OsI_28249 [Oryza sativa Indica Group])

HSP 1 Score: 166.0 bits (419), Expect = 5.7e-37
Identity = 104/344 (30.23%), Postives = 156/344 (45.35%), Query Frame = 0

Query: 3    WRVGNGKHIKIGEDPWVGEVNGYKPILLKKELAGSYVSTLIDGNGGWNENLIKESFIQAD 62
            WRVGNG+ I+I  DPW+      +PI  K      +VS L+D NG W+ + I++ F+  D
Sbjct: 846  WRVGNGRTIRIWRDPWIPRDFSRRPITHKGTSRVKWVSELLDQNGEWDSHKIQQIFLPID 905

Query: 63   AEAIARIPLGRLANQDEIIWGPDPKGAFSVKSAYLLGQSIISVGRASSSDLSESKATWRD 122
             E I  I   R    D + W  D  G FSV+SAY L  S+ +V  +SSS   E    W  
Sbjct: 906  VEKILSIHTSRFHENDFVAWHSDKLGRFSVRSAYHLALSLSNVVASSSSSGQELSKAWNQ 965

Query: 123  FWKAKSKPRETICAWRAIQNSLPTLSNIVSRGIDTNSLCFLCRKKEESGSHVFWECKFSQ 182
             W      +  I  WRA  NSL T+ N   + ++  S+C +C  +EE  +H    C  ++
Sbjct: 966  LWSCHVPQKVRIFIWRAASNSLATMVNKKKKRLEHCSMCSICGTEEEDVAHALCRCPHAK 1025

Query: 183  KIWKFFLPKVSFLLDGFRLEVNARDHWSKLRIQLKEEERDLAISIIWHIWNHRNSVKIKK 242
             +W+      +  +   R    A D    +  ++ +EER   + ++W IW  RN +   K
Sbjct: 1026 YLWEVMRRAKAITVQADRNWTGA-DWIFDISERISKEERPTLLMMLWRIWYVRNEITHGK 1085

Query: 243  --VPP--NHRAVVRDIEISLEGRRYLKNLPSPQPRSESCSSQG-------------NWIP 302
              VP   + R +   I   LE R++             C++ G              W+ 
Sbjct: 1086 AAVPAEVSQRFISSYITSLLEIRQFPDANLCKGKHVIRCAAAGAQVNHPRVNSVPVRWVR 1145

Query: 303  PPPGSWKINSDASWSDAKGIGGIGWIARDSNGSPIFAGVKRICK 330
            P  G  K+N D S+    G GGIG + R+S G  IFA    +C+
Sbjct: 1146 PQAGWMKLNVDGSYDPRDGSGGIGAVLRNSEGKLIFAACGSMCR 1188

BLAST of Lag0028898 vs. NCBI nr
Match: ABA98898.1 (retrotransposon protein, putative, unclassified [Oryza sativa Japonica Group])

HSP 1 Score: 163.7 bits (413), Expect = 2.8e-36
Identity = 98/337 (29.08%), Postives = 152/337 (45.10%), Query Frame = 0

Query: 3    WRVGNGKH---IKIGEDPWVGEVNGYKPILLKKELAGSYVSTLIDGNGGWNENLIKESFI 62
            WR+    H   IKI  DPW+      +PI  K      +V+ L+D NG W+ ++I + F 
Sbjct: 735  WRLIENPHSLSIKIWRDPWLPRDFSRRPITPKGNCRLKWVADLLDQNGSWDRDVISQLFW 794

Query: 63   QADAEAIARIPLGRLANQDEIIWGPDPKGAFSVKSAYLLGQSIISVGRASSSDLSESKAT 122
              D E I  I L    ++D I W PD  G FS++SAY+L   + + G +SSS  +++K  
Sbjct: 795  PLDGEIIKSIRLSSRQDEDFIAWYPDKMGQFSIRSAYMLAVQLANAGESSSSSGNDTKKV 854

Query: 123  WRDFWKAKSKPRETICAWRAIQNSLPTLSNIVSRGIDTNSLCFLCRKKEESGSHVFWECK 182
            W   WK     +  + +WRAI N LPT+ N   R  + + +C  C  + E  +H  W C 
Sbjct: 855  WNHVWKCNIPQKVKVFSWRAISNCLPTMVNKKKRNPELSDVCSFCGLEPEDVAHALWRCP 914

Query: 183  FSQKIWKFFLP--KVSFLLDGFRLEVNARDHWSKLRIQLKEEERDLAISIIWHIWNHRNS 242
             +++ W       +V  +L G        D        + E++R + +  +W IW  RN 
Sbjct: 915  HARRYWDLMSSEMQVQRILYG---SGEGSDWILDFLESISEDDRPVFLMSLWRIWYTRNE 974

Query: 243  VKIKKVPPNHRAVVRDIEISLEGRRYLKNLPS------------PQPRSESCSSQGNWIP 302
            +   K  P      R +   +   + +K  P+             +P+ +  S    W  
Sbjct: 975  ITHDKAAPPVEVSKRFVRSYVNSLKEIKARPNANLGSGKHVIGEDRPKCKKQSVLAKWEK 1034

Query: 303  PPPGSWKINSDASWSDAKGIGGIGWIARDSNGSPIFA 323
            PP G  K+N D S+ +   IG I  I R+S G  IFA
Sbjct: 1035 PPSGWMKLNVDGSYQE-DNIGEISAILRNSTGDVIFA 1067

BLAST of Lag0028898 vs. NCBI nr
Match: BAH93942.1 (Os07g0501200 [Oryza sativa Japonica Group])

HSP 1 Score: 163.7 bits (413), Expect = 2.8e-36
Identity = 107/350 (30.57%), Postives = 162/350 (46.29%), Query Frame = 0

Query: 3   WRVGNGKHIKIGEDPWVGEVNGYKPILLKKELAGSYVSTLIDGNGGWNENLIKESFIQAD 62
           WRVGNG  I+I  DPW+      +PI  K      +V  L++ NG W+   I++ F Q D
Sbjct: 165 WRVGNGHSIRIWRDPWLPRDFSRRPITRKGNCRIKWVPDLLNDNGEWDLERIQQHFNQID 224

Query: 63  AEAIARIPLGRLANQDEIIWGPDPKGAFSVKSAYLLGQSIISVGRASSSDLSESKATWRD 122
            + I +I   R    D + W PD  G FSV+SAY L  S+ +V  +SSS   +    W+ 
Sbjct: 225 TDIILKIRPSRSQEADFVAWHPDKHGIFSVRSAYHLAVSLANVNSSSSSSGHDLSKAWKL 284

Query: 123 FWKAKSKPRETICAWRAIQNSLPTLSNIVSRGIDTNSLCFLCRKKEESGSHVFWECKFSQ 182
            W      +  I AW+A  NSL TL N  +R ++  ++C +C ++EE  +H    C  + 
Sbjct: 285 VWNCNIPQKVKIFAWKAATNSLATLQNKKTRRLEPMAICSICGREEEDVAHALCRCPHAT 344

Query: 183 KIWKFFLPKVSFLLDGFRLEVNARDHWS-------KLRIQLKEEERDLAISIIWHIWNHR 242
            +W         L     +   A   WS       +L + + +EE  + + ++W IW +R
Sbjct: 345 NLW-------LSLRSSKDISFEAGASWSGSGWILDQLGL-IAKEEGAMFLMLLWRIWYNR 404

Query: 243 NSV-KIKKVPP---NHRAVVRDIEISLE-----------GRRYLKNLPSPQPRSESCSSQ 302
           N +   K  PP   +HR +V  +    E           G+  + NL   +  S+    +
Sbjct: 405 NEIYHGKSAPPVSVSHRFIVSYVSSLREIKQFPRADFAKGKFVVSNLGCMKGTSQVPDPK 464

Query: 303 --GNWIPPPPGSWKINSDASWSDAKGIGGIGWIARDSNGSPIFAGVKRIC 329
              +W  P PG  K+N D S+    G GGIG + R+S GS IF G    C
Sbjct: 465 VLNHWEKPNPGWMKLNVDGSFDARYGSGGIGAVLRNSAGSLIFCGNMSRC 506

BLAST of Lag0028898 vs. ExPASy TrEMBL
Match: B9FYJ3 (Uncharacterized protein OS=Oryza sativa subsp. japonica OX=39947 GN=OsJ_28268 PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 5.4e-41
Identity = 108/343 (31.49%), Postives = 164/343 (47.81%), Query Frame = 0

Query: 3    WRVGNGKHIKIGEDPWVGEVNGYKPILLKKELAGSYVSTLIDGNGGWNENLIKESFIQAD 62
            W +GNGK +++ +DPW+      +PI  K+     +VS LID +G W+ + I   F+  D
Sbjct: 800  WHIGNGKSVRLWKDPWIPRSYSRRPISAKRNCRLRWVSDLIDQSGSWDNDKISHHFLPMD 859

Query: 63   AEAIARIPLGRLANQDEIIWGPDPKGAFSVKSAYLLGQSIISVGRASSSDLSESKATWRD 122
            AEAI  I L     +D I W PD  G FSV+SAY L  ++  V   SSS  + S      
Sbjct: 860  AEAILNIHLSSRLEEDFIAWHPDRLGRFSVRSAYHLAVALAQVNDGSSSSGNGSSRACNA 919

Query: 123  FWKAKSKPRETICAWRAIQNSLPTLSNIVSRGIDTNSLCFLCRKKEESGSHVFWECKFSQ 182
             WK  +  +  I AWRAI NSL TL N   R ++ + +C +C  + E   H  + C  ++
Sbjct: 920  LWKCNAPQKVKIFAWRAITNSLTTLENKKKRKLEVSDICTICGVESEYVVHALFHCPHAR 979

Query: 183  KIWKFFLPKVSFLLDGFRLE--VNARDHWSKLRIQLKE----EERDLAISIIWHIWNHRN 242
            ++W+        + D  +L    N  +  SK+ + L E    E++ + + ++W IW+ RN
Sbjct: 980  QLWE-------AMSDDMQLNPLSNIHNGDSKVILDLLEQSQPEDQVMLLMVLWRIWHTRN 1039

Query: 243  SVKIKKVPPNHRAVVRDIEISLEGRRYLKNLPSPQP------------RSESCS-----S 302
             +   K  P      R IE  +     +K  P   P            +S S       +
Sbjct: 1040 EIVHGKPAPGILVSKRFIESYVLSLAEIKQHPQANPEKGKHVVDVVLKKSHSIKRSREPA 1099

Query: 303  QGNWIPPPPGSWKINSDASWSDAKGIGGIGWIARDSNGSPIFA 323
               W  P PGS K+N D S+ +++G GGIG + R+  G  IFA
Sbjct: 1100 PDKWSKPLPGSMKLNVDGSFQESEGKGGIGAVLRNCTGEVIFA 1135

BLAST of Lag0028898 vs. ExPASy TrEMBL
Match: A0A803PVI9 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 1.9e-38
Identity = 102/336 (30.36%), Postives = 162/336 (48.21%), Query Frame = 0

Query: 1    MRWRVGNGKHIKIGEDPWVGEVNGYKPILLKKELAGSYVSTLIDGNGGWNENLIKESFIQ 60
            +RW+VG+G++I    DPW+     +KP+         YVS+ I  +  WN  L+ ++F+ 
Sbjct: 801  LRWKVGDGQNILFASDPWIPGFTNFKPLQSVVTSTNQYVSSFITADRRWNLELLYDTFLI 860

Query: 61   ADAEAIARIPLGRLANQDEIIWGPDPKGAFSVKSAYLLGQSIISVGRASSSDLSESKATW 120
            +D E + +IPL      D++IW  +  GA++VKS Y L  S+    +  ++   + +  W
Sbjct: 861  SDVEKVLQIPLSIFPASDQLIWNFETNGAYTVKSGYALATSLEE--QMPNASGLQCQTWW 920

Query: 121  RDFWKAKSKPRETICAWRAIQNSLPTLSNIVSRGIDTNSLCFLCRKKEESGSHVFWECKF 180
              FW      +  I  WRAI + LPT + +  R I  + +C LC+   E+  H  + CK 
Sbjct: 921  NKFWSLTLPSKVRIFLWRAINDCLPTAATLHHRHIADSPICLLCQNARETTQHALFYCKR 980

Query: 181  SQKIWK---FFL-PKVSFLLDGFRLEVNARDHWSKLRIQLKEEERDLAISIIWHIWNHRN 240
             +KIW+   F L P +S  +    L +   D WS L+I       +  I+I+W IWN RN
Sbjct: 981  PRKIWRDSSFTLSPMISEYMTIKELMLQVSDLWSNLQI-------EQFITILWLIWNERN 1040

Query: 241  SVK--IKKVPPN---HRAVVRDIEISLEGRRYLKNLPSPQPRSESCSSQGNWIPPPPGSW 300
              K   K  P N   + A+    +     R+     P+    +       +W+ PP G  
Sbjct: 1041 KEKHGHKTKPYNLLEYSALSYLEDYQAAQRKKPATTPAASHNNLHQKQDASWLHPPTGRL 1100

Query: 301  KINSDASWSDAKGIGGIGWIARDSNGSPIFAGVKRI 328
            K+N+DA+ + A    G G I RDSNG  + A  K +
Sbjct: 1101 KLNTDAAINQATQTTGCGAILRDSNGEIVAAFSKPV 1127

BLAST of Lag0028898 vs. ExPASy TrEMBL
Match: B8BBX0 (Reverse transcriptase domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_28249 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 2.8e-37
Identity = 104/344 (30.23%), Postives = 156/344 (45.35%), Query Frame = 0

Query: 3    WRVGNGKHIKIGEDPWVGEVNGYKPILLKKELAGSYVSTLIDGNGGWNENLIKESFIQAD 62
            WRVGNG+ I+I  DPW+      +PI  K      +VS L+D NG W+ + I++ F+  D
Sbjct: 846  WRVGNGRTIRIWRDPWIPRDFSRRPITHKGTSRVKWVSELLDQNGEWDSHKIQQIFLPID 905

Query: 63   AEAIARIPLGRLANQDEIIWGPDPKGAFSVKSAYLLGQSIISVGRASSSDLSESKATWRD 122
             E I  I   R    D + W  D  G FSV+SAY L  S+ +V  +SSS   E    W  
Sbjct: 906  VEKILSIHTSRFHENDFVAWHSDKLGRFSVRSAYHLALSLSNVVASSSSSGQELSKAWNQ 965

Query: 123  FWKAKSKPRETICAWRAIQNSLPTLSNIVSRGIDTNSLCFLCRKKEESGSHVFWECKFSQ 182
             W      +  I  WRA  NSL T+ N   + ++  S+C +C  +EE  +H    C  ++
Sbjct: 966  LWSCHVPQKVRIFIWRAASNSLATMVNKKKKRLEHCSMCSICGTEEEDVAHALCRCPHAK 1025

Query: 183  KIWKFFLPKVSFLLDGFRLEVNARDHWSKLRIQLKEEERDLAISIIWHIWNHRNSVKIKK 242
             +W+      +  +   R    A D    +  ++ +EER   + ++W IW  RN +   K
Sbjct: 1026 YLWEVMRRAKAITVQADRNWTGA-DWIFDISERISKEERPTLLMMLWRIWYVRNEITHGK 1085

Query: 243  --VPP--NHRAVVRDIEISLEGRRYLKNLPSPQPRSESCSSQG-------------NWIP 302
              VP   + R +   I   LE R++             C++ G              W+ 
Sbjct: 1086 AAVPAEVSQRFISSYITSLLEIRQFPDANLCKGKHVIRCAAAGAQVNHPRVNSVPVRWVR 1145

Query: 303  PPPGSWKINSDASWSDAKGIGGIGWIARDSNGSPIFAGVKRICK 330
            P  G  K+N D S+    G GGIG + R+S G  IFA    +C+
Sbjct: 1146 PQAGWMKLNVDGSYDPRDGSGGIGAVLRNSEGKLIFAACGSMCR 1188

BLAST of Lag0028898 vs. ExPASy TrEMBL
Match: A0A803PJK4 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 6.2e-37
Identity = 102/340 (30.00%), Postives = 156/340 (45.88%), Query Frame = 0

Query: 1    MRWRVGNGKHIKIGEDPWVGEVNGYKPILLKKELAGSYVSTLIDGNGGWNENLIKESFIQ 60
            +RW+VGNG  IK   DPW+     +KP+L K       VS LID +  W+   +K +F+ 
Sbjct: 1398 LRWKVGNGLQIKCATDPWLPSHTSFKPLLFKGSDFNLTVSELIDCDRQWHMPSLKSNFLD 1457

Query: 61   ADAEAIARIPLGRLANQDEIIWGPDPKGAFSVKSAYLLGQSIISVGRASSSDLSESKATW 120
             D + I  IPL   ++ D+IIW  +  G ++VKS Y+L          S S  S S   W
Sbjct: 1458 IDIDKILSIPLSTFSHDDKIIWHHETTGIYTVKSGYILASKFADQEAPSVS--SHSTQLW 1517

Query: 121  RDFWKAKSKPRETICAWRAIQNSLPTLSNIVSRGIDTNSLCFLCRKKEESGSHVFWECKF 180
            + FWK     +  I  W+A+Q  LP  + +    I  + +C LCR   ES  H  + CK 
Sbjct: 1518 KHFWKLNIPSKVRIFLWKAVQECLPVAAILQKCHIAGSDICSLCRGNNESIVHALFLCKR 1577

Query: 181  SQKIWKFFLPKVSFLLDGFRLEVNA-RDHWSKLRIQLKEEERDLAISIIWHIWNHRNSVK 240
            ++K+W+      SF +D    E +  +D + K      ++E +    I+W IW  RN   
Sbjct: 1578 AKKVWR----GSSFSIDRIIHESSTFKDLFFKASETWPQQELEQFACILWSIWTERNKEY 1637

Query: 241  IKKVPPNHRAVVRDI-----EISLEGRRYLKNLPSPQPRSESCSSQGNWIPPPPGSWKIN 300
                P  H  ++        E     +    +L +   R+   +    W+ PP G  K+N
Sbjct: 1638 HGTKPKPHEMLLVSALSYLGEYQTSRQATPTHLRNEVHRNNEPTVAPKWLNPPSGRLKLN 1697

Query: 301  SDASWSDAKGIGGIGWIARDSNGSPIFAGVKRICKKWPSC 335
            +DA+  +   I G G I RDS+G  + A    + K  P C
Sbjct: 1698 TDAAVDNVNRISGFGAILRDSDGQVVAA----MSKPHPGC 1727

BLAST of Lag0028898 vs. ExPASy TrEMBL
Match: C7J4L5 (Os07g0501200 protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0501200 PE=4 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 1.4e-36
Identity = 107/350 (30.57%), Postives = 162/350 (46.29%), Query Frame = 0

Query: 3   WRVGNGKHIKIGEDPWVGEVNGYKPILLKKELAGSYVSTLIDGNGGWNENLIKESFIQAD 62
           WRVGNG  I+I  DPW+      +PI  K      +V  L++ NG W+   I++ F Q D
Sbjct: 165 WRVGNGHSIRIWRDPWLPRDFSRRPITRKGNCRIKWVPDLLNDNGEWDLERIQQHFNQID 224

Query: 63  AEAIARIPLGRLANQDEIIWGPDPKGAFSVKSAYLLGQSIISVGRASSSDLSESKATWRD 122
            + I +I   R    D + W PD  G FSV+SAY L  S+ +V  +SSS   +    W+ 
Sbjct: 225 TDIILKIRPSRSQEADFVAWHPDKHGIFSVRSAYHLAVSLANVNSSSSSSGHDLSKAWKL 284

Query: 123 FWKAKSKPRETICAWRAIQNSLPTLSNIVSRGIDTNSLCFLCRKKEESGSHVFWECKFSQ 182
            W      +  I AW+A  NSL TL N  +R ++  ++C +C ++EE  +H    C  + 
Sbjct: 285 VWNCNIPQKVKIFAWKAATNSLATLQNKKTRRLEPMAICSICGREEEDVAHALCRCPHAT 344

Query: 183 KIWKFFLPKVSFLLDGFRLEVNARDHWS-------KLRIQLKEEERDLAISIIWHIWNHR 242
            +W         L     +   A   WS       +L + + +EE  + + ++W IW +R
Sbjct: 345 NLW-------LSLRSSKDISFEAGASWSGSGWILDQLGL-IAKEEGAMFLMLLWRIWYNR 404

Query: 243 NSV-KIKKVPP---NHRAVVRDIEISLE-----------GRRYLKNLPSPQPRSESCSSQ 302
           N +   K  PP   +HR +V  +    E           G+  + NL   +  S+    +
Sbjct: 405 NEIYHGKSAPPVSVSHRFIVSYVSSLREIKQFPRADFAKGKFVVSNLGCMKGTSQVPDPK 464

Query: 303 --GNWIPPPPGSWKINSDASWSDAKGIGGIGWIARDSNGSPIFAGVKRIC 329
              +W  P PG  K+N D S+    G GGIG + R+S GS IF G    C
Sbjct: 465 VLNHWEKPNPGWMKLNVDGSFDARYGSGGIGAVLRNSAGSLIFCGNMSRC 506

BLAST of Lag0028898 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 100.5 bits (249), Expect = 2.7e-21
Identity = 81/338 (23.96%), Postives = 144/338 (42.60%), Query Frame = 0

Query: 2   RWRVGNGKHIKIGEDPWVGEVNGYKPILLKKELAGSYVSTLIDGNGG---WNENLIKESF 61
           R  +G+G++I+IG D  V + +  +P+  ++      ++ L +  G    W+++ I +  
Sbjct: 39  RHLIGDGQNIRIGLDNIV-DSHPPRPLNTEETYKEMTINNLFERKGSYYFWDDSKISQFV 98

Query: 62  IQADAEAIARIPLGRLANQDEIIWGPDPKGAFSVKSAYLLGQSIISVGRASSSDLSESKA 121
            Q+D   I RI L +    D+IIW  +  G ++V+S Y L     S    + +    S  
Sbjct: 99  DQSDHGFIHRIYLAKSKKPDKIIWNYNTTGEYTVRSGYWLLTHDPSTNIPAINPPHGSID 158

Query: 122 TWRDFWKAKSKPRETICAWRAIQNSLPTLSNIVSRGIDTNSLCFLCRKKEESGSHVFWEC 181
                W     P+     WRA+  +L T   + +RG+  +  C  C ++ ES +H  + C
Sbjct: 159 LKTRIWNLPIMPKLKHFLWRALSQALATTERLTTRGMRIDPSCPRCHRENESINHALFTC 218

Query: 182 KFSQKIWKFFLPKVSFLLDGFRLEVNARDHWSKL-----RIQLKEEERDLAISIIWHIWN 241
            F+   W+      S L+    +  +  ++ S +        + +  + L + +IW IW 
Sbjct: 219 PFATMAWRL---SDSSLIRNQLMSNDFEENISNILNFVQDTTMSDFHKLLPVWLIWRIWK 278

Query: 242 HRNSVKIKKVPPNHRAVVRDIEISLEGRRYLKNLPS----PQPRSESCSSQGNWIPPPPG 301
            RN+V   K   +    V  +    E   +L    S    P P  +   ++  W  PP  
Sbjct: 279 ARNNVVFNKFRESPSKTV--LSAKAETHDWLNATQSHKKTPSPTRQIAENKIEWRNPPAT 338

Query: 302 SWKINSDASWSDAKGIGGIGWIARDSNGSPIFAGVKRI 328
             K N DA +   K     GWI R+  G+PI  G  ++
Sbjct: 339 YVKCNFDAGFDVQKLEATGGWIIRNHYGTPISWGSMKL 370

BLAST of Lag0028898 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 3.0e-12
Identity = 51/210 (24.29%), Postives = 82/210 (39.05%), Query Frame = 0

Query: 124 WKAKSKPRETICAWRAIQNSLPTLSNIVSRGIDTNSLCFLCRKKEESGSHVFWECKFSQK 183
           WK    P+     WR +  +L T + + SR ID + +C  C  +EE+  H+ + C ++Q 
Sbjct: 38  WKLHVAPKIKHFLWRCVTGALATNTRLRSRNIDADPICQRCCIEEETIHHIMFNCPYTQS 97

Query: 184 IWK---FFLPKVSFLLDGFRLEVNARDHWSKLRIQLKEEERDLAISIIWHIWNHRN---- 243
           +W+     +         F   +N     SK +      +R L   I+W +W  RN    
Sbjct: 98  VWRSANIIIGNQWGPPSSFEDNLNRLIQLSKTQ-TTNSLDRFLPFWIMWRLWKSRNVFLF 157

Query: 244 SVKIKKVPPNHRAVVRDIEISLEGRRYLKNL---PSPQPRSESCSSQGNWIPPPPGSWKI 303
             K +      R  ++D    L      +N     +  P   S      W PPP G  K 
Sbjct: 158 QQKCQSPDYEARKGIQDATEWLNANETTENTNVHVATNPIQTSRRDSSQWNPPPEGWVKC 217

Query: 304 NSDASWSDAKGIGGIGWIARDSNGSPIFAG 324
           N D+ ++        GW  R+ NG  +  G
Sbjct: 218 NFDSGYTQGSPYTRSGWTIRECNGHIVLCG 246

BLAST of Lag0028898 vs. TAIR 10
Match: AT5G18880.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 65.5 bits (158), Expect = 9.8e-11
Identity = 65/289 (22.49%), Postives = 116/289 (40.14%), Query Frame = 0

Query: 1   MRWRVGNGKHIKIGEDPWV--GEVNGYKPILLKKELAGSYVSTLIDG--NGGWNENLIKE 60
           MR  +GNG+      D W   G++  +      ++L     + +++   NG W     + 
Sbjct: 12  MRCDMGNGESASFWYDAWTDFGQLLTFLGAAGPRQLRIRQDARVVEASRNGDWFLPAARS 71

Query: 61  SFIQADAEAIARIPLGRLA-NQDEIIWGPDPKGAFSVKSAYLLGQSIISVGRASSSDLSE 120
              Q    A+   P+   +  QD  +W        +   +YL   S  S        +  
Sbjct: 72  DNSQLFLAALTMAPVPHESRGQDSFLWR-------NAAGSYL--PSFSSRDTWEQIRVHS 131

Query: 121 SKATW-RDFWKAKSKPRETICAWRAIQNSLPTLSNIVSRGIDTNSLCFLCRKKEESGSHV 180
               W +  W  +  PR ++  W +    LPT   +   G++  S   LC   +E+ +H+
Sbjct: 132 PTVPWAKVVWFKEYIPRFSLITWMSFLERLPTRDRLRGWGMNIPSSWVLCSNGDETHAHL 191

Query: 181 FWECKFSQKIWKFFL----PKVSFLL---DGFRLEVNARDHWSKLRIQLKEEERDLAISI 240
           F+EC FS  IW+FF     P   F L     + L++  R H + +   L +       S 
Sbjct: 192 FFECSFSLAIWEFFASKFRPSPPFGLPAASSWILQLPLRSHSTTILKLLLQ-------SA 251

Query: 241 IWHIWNHRNSVKIKKVPPNHRAVVRDIEISLEGRRYLKNLPSPQPRSES 277
           ++H+W  RN+     +  +  ++   I+ ++  R  L + PS   RS S
Sbjct: 252 VYHVWKERNARIFTSISSSASSLRLAIDRTMRNR--LLSFPSADLRSPS 282

BLAST of Lag0028898 vs. TAIR 10
Match: AT1G43730.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 60.8 bits (146), Expect = 2.4e-09
Identity = 37/148 (25.00%), Postives = 65/148 (43.92%), Query Frame = 0

Query: 120 WRDFWKAKSKPRETICAWRAIQNSLPTLSNIVSRGIDTNSLCFLCRKKEESGSHVFWECK 179
           ++  W     P+     W    N L T   + S G+   ++C LC   +ES +H+F+EC 
Sbjct: 152 YKAVWFKNHVPKHAFICWVVAWNRLHTRDRLRSWGLSIPAVCLLCNSHDESRAHLFFECP 211

Query: 180 FSQKIWKFF------LPKVSFLLDGFRLEVNARDHWSKLRIQLKEEERDLAISIIWHIWN 239
           F   +W+FF       P    +     L   +RD  + L I+L  +      + ++ IW 
Sbjct: 212 FCGAVWRFFTGRANLFPPAQLMYCLIWLLNPSRDKNTTLIIRLAFQ------AYVYAIWR 271

Query: 240 HRNSVKIKKVPPNHRAVVRDIEISLEGR 262
            RN      V     +V++DI++++  R
Sbjct: 272 ERNLCLHTGVARVFDSVLKDIQLTIRAR 293

BLAST of Lag0028898 vs. TAIR 10
Match: AT4G04650.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 58.9 bits (141), Expect = 9.2e-09
Identity = 34/143 (23.78%), Postives = 61/143 (42.66%), Query Frame = 0

Query: 124 WKAKSKPRETICAWRAIQNSLPTLSNIVSRGIDTNSLCFLCRKKEESGSHVFWECKFSQK 183
           W     P+     W    N L T   + + G+   + C LC   ++S +H+F+EC+FS  
Sbjct: 134 WFKNHVPKHAFICWVVAWNRLHTRDRLQNWGLSIPAECLLCNAHDDSRAHLFFECQFSGV 193

Query: 184 IWKFFLPKVSFLLDGFRLEVNARDHWSKLRIQLKEEERDLAISIIWH-----IWNHRNSV 243
           +W+FF    +             D  + L    +E+   L I + +H     IW  RN  
Sbjct: 194 VWRFFTASTN-----LNPPAQLMDCLNWLLSPSREKNICLIIRLAFHSCVYAIWRERNQR 253

Query: 244 KIKKVPPNHRAVVRDIEISLEGR 262
               V  +  ++++DI++ +  R
Sbjct: 254 LHSGVSRSTESILKDIQLIIRAR 271

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
EEE69144.11.1e-4031.49hypothetical protein OsJ_28268 [Oryza sativa Japonica Group][more]
XP_024038343.12.5e-4033.44uncharacterized protein LOC112097373 [Citrus clementina][more]
EEC83100.15.7e-3730.23hypothetical protein OsI_28249 [Oryza sativa Indica Group][more]
ABA98898.12.8e-3629.08retrotransposon protein, putative, unclassified [Oryza sativa Japonica Group][more]
BAH93942.12.8e-3630.57Os07g0501200 [Oryza sativa Japonica Group][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
B9FYJ35.4e-4131.49Uncharacterized protein OS=Oryza sativa subsp. japonica OX=39947 GN=OsJ_28268 PE... [more]
A0A803PVI91.9e-3830.36Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
B8BBX02.8e-3730.23Reverse transcriptase domain-containing protein OS=Oryza sativa subsp. indica OX... [more]
A0A803PJK46.2e-3730.00Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
C7J4L51.4e-3630.57Os07g0501200 protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0501200 PE... [more]
Match NameE-valueIdentityDescription
AT3G09510.12.7e-2123.96Ribonuclease H-like superfamily protein [more]
AT2G02650.13.0e-1224.29Ribonuclease H-like superfamily protein [more]
AT5G18880.19.8e-1122.49RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT1G43730.12.4e-0925.00RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT4G04650.19.2e-0923.78RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 90..185
e-value: 3.8E-18
score: 66.0
NoneNo IPR availablePANTHERPTHR46736FAMILY NOT NAMEDcoord: 2..320
NoneNo IPR availablePANTHERPTHR46736:SF6SUBFAMILY NOT NAMEDcoord: 2..320

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0028898.1Lag0028898.1mRNA