Lag0028821 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0028821
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionupstream activation factor subunit UAF30
Locationchr8: 31337461 .. 31339300 (+)
RNA-Seq ExpressionLag0028821
SyntenyLag0028821
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGCCACAACGATTGAAGACGACCATTACAGATAACCCAAAGAAGCTTGCCAACTTGATTGATCTTGTAAACCTTCCTTCAACTCTCAGAGACTTTGTGGGACAGTCCCAAATTTCTCGTCTGGGCTGTTTCAAATGTGTGTGGTCTTATATCAAAACCAACAATCTTCAGGTTCTTTTTTGTCTCATTTTATGGGTTTTCTGATTAGTTTTAGATGCATATTGATTATATTGGTATCAAAAAGAGACTTCCCTTGTTAAAAACTCAGATCTACTTGAAAAATTTTGTTATATATTCTACATTTTGTTCTGGTTAAAGTATAGTTTAAAAAGGAGAGGAGGAAGAGGGATAAGAGCAATCAAAGAGGAGAAGAAAGATCTGTAGGACATAATTGGAGTTAGGTAAAGACAGATATCATGCAACAACGGGGGAAGTGAAGATAAGTCTGAAGACTCAGGATGAAAGAGGATCTCCTGAAATGAAAGTAATGAGAAACTGATGGAGGGGTTTAACACAAAATACAGTTTGATAAAAGCATCTTTTTGCAACGAAAGATTGGATAAACTGAGATAGAGGTTACAAAGAGGGTCTAATATAGCTCAAACAGAACTAGAGTTATAATAAGATATAGAAGATAGGAGAACTACAAGATAACCCAAAGGTACCTGAACCTCCTTTCATTGAGTATACACACTCAAGCCTAAACCATAATAACAATTCTTTAGCTTCATCCTTTACTACTCACTCTATTTATAACAGGTCCCCTAACAAACTCCTTGATCTAATTACTAATATGCCCTCAATACCCTAATAATACTCCTTACAACAATAGTATTCCTATCAGAGAAGAGATGCTTTTGACAGTTTTTTCAACCACATGGTCACCAAAGGAACACGATCTTGAGTTATGACCCGAGGGAAGACCTAAGCCTAAATAGGTCATAGGCAACATAGAGGACCTCACAACCCTGCAGATGAACCCACCTCTCAAGCTTGACTGGCTCACTATTAACACTGACTAACAAATATTTCTCGTTATTAAATACTACCAGAAATGACTTCAAAAACCTATAAAGTTCTATTAAGGCTCACAAATGACTCCTCCCTCCGGAAGCATATGGAAATAGTATTGTAACTGTAAATAAGTTAGATAGATGATGATGACTTCTCTCCTTACCAATAAAAGCCCTTCTCTATTAAACATTTATCCATTCTTTTCAAAACTAAGTCTGTTGAATCCGCAACCACTTGAAAAGAAAATGATGCTTGATTTTACTTGCAAATTCAGTGACTTGTTTTTTGCACCAATTTGTAGTACAGTAGACTTTATGACTTTTTTCTTTGACAAGTACACTTTATGACTGTTTTCCAGCTTCCCAAACTCTTATCATATAACTGTTCCATTGCCATTGGATATGAGTTTTGATAAGCTGGAAGTACTTGAAACTTTAAAGTAGCAGCACTTCTTTTTTTTTTTTTTTTCTAACTCTTATATATATGTTTTCTGTTGGCCGATTAGATGTGTTTCTCCATTCTGATAAAAAGATTATGGATATGTACCTTTCTTGTCTAGGATCCGACCAACAAGAATGTGGTCATTTGTGATGAGAAGTTAAAGAGTATCTTGCTAGGCAAGCCCAAGGTCGAGCTAGCCGAACTTCCTGCCCTGATTAAATTACATTTCCCCAAAAACCCCAAGTGAGTGATGCTTGCATAGCACATCCCCAATGACGTCTCCAAGTCGCCGATATTTAGATTGTTTAGCCTTAATTTTGTACTATTGTCCTACAGGTTTCTTTCACATATATTATCAGAGAATTATTTGACTTTTGCATAG

mRNA sequence

ATGCTGCCACAACGATTGAAGACGACCATTACAGATAACCCAAAGAAGCTTGCCAACTTGATTGATCTTGTAAACCTTCCTTCAACTCTCAGAGACTTTGTGGGACAGTCCCAAATTTCTCGTCTGGGCTGTTTCAAATGTGTGTGGTCTTATATCAAAACCAACAATCTTCAGGATCCGACCAACAAGAATGTGGTCATTTGTGATGAGAAGTTAAAGAGTATCTTGCTAGGCAAGCCCAAGGTCGAGCTAGCCGAACTTCCTGCCCTGATTAAATTACATTTCCCCAAAAACCCCAAGTTTCTTTCACATATATTATCAGAGAATTATTTGACTTTTGCATAG

Coding sequence (CDS)

ATGCTGCCACAACGATTGAAGACGACCATTACAGATAACCCAAAGAAGCTTGCCAACTTGATTGATCTTGTAAACCTTCCTTCAACTCTCAGAGACTTTGTGGGACAGTCCCAAATTTCTCGTCTGGGCTGTTTCAAATGTGTGTGGTCTTATATCAAAACCAACAATCTTCAGGATCCGACCAACAAGAATGTGGTCATTTGTGATGAGAAGTTAAAGAGTATCTTGCTAGGCAAGCCCAAGGTCGAGCTAGCCGAACTTCCTGCCCTGATTAAATTACATTTCCCCAAAAACCCCAAGTTTCTTTCACATATATTATCAGAGAATTATTTGACTTTTGCATAG

Protein sequence

MLPQRLKTTITDNPKKLANLIDLVNLPSTLRDFVGQSQISRLGCFKCVWSYIKTNNLQDPTNKNVVICDEKLKSILLGKPKVELAELPALIKLHFPKNPKFLSHILSENYLTFA
Homology
[Close]
BLAST of Lag0028821 vs. NCBI nr
Match: XP_038882021.1 (upstream activation factor subunit spp27 [Benincasa hispida])

HSP 1 Score: 197.2 bits (500), Expect = 7.6e-47
Identity = 95/100 (95.00%), Postives = 98/100 (98.00%), Query Frame = 0 

Query: 1   MLPQRLKTTITDNPKKLANLIDLVNLPSTLRDFVGQSQISRLGCFKCVWSYIKTNNLQDP 60
           MLP+R+K  ITDNPKKLANLIDLVNLPSTLR+FVGQSQISRLGCFKCVWSYIKTNNLQDP
Sbjct: 1   MLPKRMKMAITDNPKKLANLIDLVNLPSTLREFVGQSQISRLGCFKCVWSYIKTNNLQDP 60

Query: 61  TNKNVVICDEKLKSILLGKPKVELAELPALIKLHFPKNPK 101
           TNKNVVICDEKLKSILLGKPKVELAELPALIKLHFPKNPK
Sbjct: 61  TNKNVVICDEKLKSILLGKPKVELAELPALIKLHFPKNPK 100

[Close]
BLAST of Lag0028821 vs. NCBI nr
Match: XP_022928748.1 (upstream activation factor subunit UAF30 [Cucurbita moschata] >XP_022928749.1 upstream activation factor subunit UAF30 [Cucurbita moschata])

HSP 1 Score: 193.4 bits (490), Expect = 1.1e-45
Identity = 94/100 (94.00%), Postives = 97/100 (97.00%), Query Frame = 0 

Query: 1   MLPQRLKTTITDNPKKLANLIDLVNLPSTLRDFVGQSQISRLGCFKCVWSYIKTNNLQDP 60
           MLPQR+KT ITDNPKKLANLIDLVNLPSTLR+FVGQSQISRLGCFK VWSYIKTNNLQDP
Sbjct: 1   MLPQRMKTAITDNPKKLANLIDLVNLPSTLREFVGQSQISRLGCFKYVWSYIKTNNLQDP 60

Query: 61  TNKNVVICDEKLKSILLGKPKVELAELPALIKLHFPKNPK 101
           TNKNVVICDEKLKSILLGK K+ELAELPALIKLHFPKNPK
Sbjct: 61  TNKNVVICDEKLKSILLGKSKIELAELPALIKLHFPKNPK 100

[Close]
BLAST of Lag0028821 vs. NCBI nr
Match: KAG6603170.1 (hypothetical protein SDJN03_03779, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 193.4 bits (490), Expect = 1.1e-45
Identity = 94/100 (94.00%), Postives = 97/100 (97.00%), Query Frame = 0 

Query: 1   MLPQRLKTTITDNPKKLANLIDLVNLPSTLRDFVGQSQISRLGCFKCVWSYIKTNNLQDP 60
           MLPQR+KT ITDNPKKLANLIDLVNLPSTLR+FVGQSQISRLGCFK VWSYIKTNNLQDP
Sbjct: 31  MLPQRMKTAITDNPKKLANLIDLVNLPSTLREFVGQSQISRLGCFKYVWSYIKTNNLQDP 90

Query: 61  TNKNVVICDEKLKSILLGKPKVELAELPALIKLHFPKNPK 101
           TNKNVVICDEKLKSILLGK K+ELAELPALIKLHFPKNPK
Sbjct: 91  TNKNVVICDEKLKSILLGKSKIELAELPALIKLHFPKNPK 130

[Close]
BLAST of Lag0028821 vs. NCBI nr
Match: XP_022967914.1 (upstream activation factor subunit UAF30 isoform X2 [Cucurbita maxima] >XP_022967915.1 upstream activation factor subunit UAF30 isoform X2 [Cucurbita maxima])

HSP 1 Score: 192.6 bits (488), Expect = 1.9e-45
Identity = 94/100 (94.00%), Postives = 97/100 (97.00%), Query Frame = 0 

Query: 1   MLPQRLKTTITDNPKKLANLIDLVNLPSTLRDFVGQSQISRLGCFKCVWSYIKTNNLQDP 60
           MLPQR+KT ITDNPKKLANLIDLVNLPSTLR+FVGQSQISRLGCFK VWSYIKTNNLQDP
Sbjct: 1   MLPQRMKTAITDNPKKLANLIDLVNLPSTLREFVGQSQISRLGCFKYVWSYIKTNNLQDP 60

Query: 61  TNKNVVICDEKLKSILLGKPKVELAELPALIKLHFPKNPK 101
           TNKNVVICDEKL+SILLGK KVELAELPALIKLHFPKNPK
Sbjct: 61  TNKNVVICDEKLRSILLGKSKVELAELPALIKLHFPKNPK 100

[Close]
BLAST of Lag0028821 vs. NCBI nr
Match: XP_022967913.1 (protein TRI1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 192.6 bits (488), Expect = 1.9e-45
Identity = 94/100 (94.00%), Postives = 97/100 (97.00%), Query Frame = 0 

Query: 1   MLPQRLKTTITDNPKKLANLIDLVNLPSTLRDFVGQSQISRLGCFKCVWSYIKTNNLQDP 60
           MLPQR+KT ITDNPKKLANLIDLVNLPSTLR+FVGQSQISRLGCFK VWSYIKTNNLQDP
Sbjct: 56  MLPQRMKTAITDNPKKLANLIDLVNLPSTLREFVGQSQISRLGCFKYVWSYIKTNNLQDP 115

Query: 61  TNKNVVICDEKLKSILLGKPKVELAELPALIKLHFPKNPK 101
           TNKNVVICDEKL+SILLGK KVELAELPALIKLHFPKNPK
Sbjct: 116 TNKNVVICDEKLRSILLGKSKVELAELPALIKLHFPKNPK 155

[Close]
BLAST of Lag0028821 vs. ExPASy Swiss-Prot
Match: O74503 (Upstream activation factor subunit spp27 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=spp27 PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 2.8e-07
Identity = 23/52 (44.23%), Postives = 35/52 (67.31%), Query Frame = 0 

Query: 24  VNLPSTLRDFVGQSQISRLGCFKCVWSYIKTNNLQDPTNKNVVICDEKLKSI 76
           + L   L +F+G  Q+SR    K +W YIK ++LQDP +K  ++CD+KLKS+
Sbjct: 121 MKLSPKLAEFLGLEQLSRPQTVKKLWEYIKAHDLQDPNDKRTILCDDKLKSV 172

[Close]
BLAST of Lag0028821 vs. ExPASy Swiss-Prot
Match: Q08747 (Upstream activation factor subunit UAF30 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=UAF30 PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 4.7e-07
Identity = 31/100 (31.00%), Postives = 58/100 (58.00%), Query Frame = 0 

Query: 4   QRLKTTITDNPKKLANLIDLVNLPSTLRDFVGQSQISRLGCFKCVWSYIKTNNLQDPTNK 63
           ++ K T++ +P         V L  +L   +G+ +++R    + +W+YIK +NLQ+P NK
Sbjct: 108 EKKKGTVSKSPISTRK----VTLSKSLASLLGEHELTRTEVVRRLWAYIKAHNLQNPNNK 167

Query: 64  NVVICDEKLKSILLGKPKVELAELPALIKLHFPKNPKFLS 104
             ++CDEKL+ ++LGK    + E+  ++  H  + PK +S
Sbjct: 168 KEILCDEKLE-LILGK-STNMFEMHKILASHMTE-PKKIS 200

[Close]
BLAST of Lag0028821 vs. ExPASy Swiss-Prot
Match: Q05024 (Protein TRI1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TRI1 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 5.2e-06
Identity = 27/93 (29.03%), Postives = 51/93 (54.84%), Query Frame = 0 

Query: 12  DNPKKLANLIDLVNLPSTLRDFVGQSQISRLGCFKCVWSYIKTNNLQDPTNKNVVICDEK 71
           D+P   +  +  V L + L+ F+G  ++ R    K +W YIK ++LQ+P ++  ++CDEK
Sbjct: 112 DSPDSNSISVRKVLLSAPLQKFLGSEELPRTQVVKMIWQYIKEHDLQNPKDRREILCDEK 171

Query: 72  LKSILLGKPKVELAELPALIKLHFPKNPKFLSH 105
           ++ I  GK K+ +  +  L+  H     + + H
Sbjct: 172 MEPI-FGK-KMTMFSMNKLLTKHLFNPDEIVKH 202

[Close]
BLAST of Lag0028821 vs. ExPASy Swiss-Prot
Match: Q2TBN1 (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 OS=Bos taurus OX=9913 GN=SMARCD1 PE=2 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 2.2e-04
Identity = 23/63 (36.51%), Postives = 39/63 (61.90%), Query Frame = 0 

Query: 46  KCVWSYIKTNNLQDPTNKNVVICDEKLKSILLGKPKVELAELP-ALIKLHFPKNPKFLSH 105
           + +W YIKT+ LQDP  +  VICD+ L+ I   + +++ +E+P  L  L  P  P  ++H
Sbjct: 317 QALWQYIKTHKLQDPHEREFVICDKYLQQIFESQ-RMKFSEIPQRLHALLMPPEPIIINH 376

Query: 106 ILS 108
           ++S
Sbjct: 377 VIS 378

[Close]
BLAST of Lag0028821 vs. ExPASy Swiss-Prot
Match: Q96GM5 (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 OS=Homo sapiens OX=9606 GN=SMARCD1 PE=1 SV=2)

HSP 1 Score: 46.6 bits (109), Expect = 2.2e-04
Identity = 23/63 (36.51%), Postives = 39/63 (61.90%), Query Frame = 0 

Query: 46  KCVWSYIKTNNLQDPTNKNVVICDEKLKSILLGKPKVELAELP-ALIKLHFPKNPKFLSH 105
           + +W YIKT+ LQDP  +  VICD+ L+ I   + +++ +E+P  L  L  P  P  ++H
Sbjct: 317 QALWQYIKTHKLQDPHEREFVICDKYLQQIFESQ-RMKFSEIPQRLHALLMPPEPIIINH 376

Query: 106 ILS 108
           ++S
Sbjct: 377 VIS 378

The following BLAST results are available for this feature:
BLAST of Lag0028821 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038882021.17.6e-4795.00upstream activation factor subunit spp27 [Benincasa hispida][more]
XP_022928748.11.1e-4594.00upstream activation factor subunit UAF30 [Cucurbita moschata] >XP_022928749.1 up... [more]
KAG6603170.11.1e-4594.00hypothetical protein SDJN03_03779, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022967914.11.9e-4594.00upstream activation factor subunit UAF30 isoform X2 [Cucurbita maxima] >XP_02296... [more]
XP_022967913.11.9e-4594.00protein TRI1 isoform X1 [Cucurbita maxima][more]
BLAST of Lag0028821 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O745032.8e-0744.23Upstream activation factor subunit spp27 OS=Schizosaccharomyces pombe (strain 97... [more]
Q087474.7e-0731.00Upstream activation factor subunit UAF30 OS=Saccharomyces cerevisiae (strain ATC... [more]
Q050245.2e-0629.03Protein TRI1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 ... [more]
Q2TBN12.2e-0436.51SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfami... [more]
Q96GM52.2e-0436.51SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfami... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019835SWIB domainSMARTSM00151swib_2coord: 20..100
e-value: 1.0E-11
score: 55.0
IPR003121SWIB/MDM2 domainPFAMPF02201SWIBcoord: 24..95
e-value: 1.7E-22
score: 79.0
IPR003121SWIB/MDM2 domainPROSITEPS51925SWIB_MDM2coord: 19..97
score: 18.151392
IPR036885SWIB/MDM2 domain superfamilyGENE3D1.10.245.10SWIB/MDM2 domaincoord: 16..103
e-value: 1.6E-21
score: 78.1
IPR036885SWIB/MDM2 domain superfamilySUPERFAMILY47592SWIB/MDM2 domaincoord: 13..100
NoneNo IPR availablePANTHERPTHR13844SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY Dcoord: 2..103
NoneNo IPR availablePANTHERPTHR13844:SF45OS09G0133000 PROTEINcoord: 2..103
NoneNo IPR availableCDDcd10567SWIB-MDM2_likecoord: 24..95
e-value: 1.66093E-22
score: 81.4357

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0028821.1Lag0028821.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus