Lag0028806 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0028806
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr8: 30975398 .. 30975796 (-)
RNA-Seq ExpressionLag0028806
SyntenyLag0028806
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGACAGATGCTGCGTGGATGGTTGGGACGTTAGCCACGGGTTACGGGTGGCATATGGAGGAACATGGTGGAGGTGGTGTCAGTGAAGGAGCACAGCCAGGTGGCAAATGCCTCAGCAGTCTTCATGCTGAGGGTGGTGCACTGTTATGGGGATTAAAATGTGCAAAGAGGAAAAATATGAGAAGGCTTGTGGCCAAGTCTGATTGTTTGAGATTGATCCAAATTTTGGGGAAGAAGGAATTATGTCCAGTGGATTTTGAGCCTATTTTTGACCAAATAATGGAGATGTATGGCTTTTTTAGTTCTTGTCAATTCATGTTTGTGAGACGAGATGATAATGTAAAGGCGCATTGCCTAGCCAAACATGGCCTTTATTTACTGAGTTGCTCTGAATAA

mRNA sequence

ATGTGGACAGATGCTGCGTGGATGGTTGGGACGTTAGCCACGGGTTACGGGTGGCATATGGAGGAACATGGTGGAGGTGGTGTCAGTGAAGGAGCACAGCCAGGTGGCAAATGCCTCAGCAGTCTTCATGCTGAGGGTGGTGCACTGTTATGGGGATTAAAATGTGCAAAGAGGAAAAATATGAGAAGGCTTGTGGCCAAGTCTGATTGTTTGAGATTGATCCAAATTTTGGGGAAGAAGGAATTATGTCCAGTGGATTTTGAGCCTATTTTTGACCAAATAATGGAGATGTATGGCTTTTTTAGTTCTTGTCAATTCATGTTTGTGAGACGAGATGATAATGTAAAGGCGCATTGCCTAGCCAAACATGGCCTTTATTTACTGAGTTGCTCTGAATAA

Coding sequence (CDS)

ATGTGGACAGATGCTGCGTGGATGGTTGGGACGTTAGCCACGGGTTACGGGTGGCATATGGAGGAACATGGTGGAGGTGGTGTCAGTGAAGGAGCACAGCCAGGTGGCAAATGCCTCAGCAGTCTTCATGCTGAGGGTGGTGCACTGTTATGGGGATTAAAATGTGCAAAGAGGAAAAATATGAGAAGGCTTGTGGCCAAGTCTGATTGTTTGAGATTGATCCAAATTTTGGGGAAGAAGGAATTATGTCCAGTGGATTTTGAGCCTATTTTTGACCAAATAATGGAGATGTATGGCTTTTTTAGTTCTTGTCAATTCATGTTTGTGAGACGAGATGATAATGTAAAGGCGCATTGCCTAGCCAAACATGGCCTTTATTTACTGAGTTGCTCTGAATAA

Protein sequence

MWTDAAWMVGTLATGYGWHMEEHGGGGVSEGAQPGGKCLSSLHAEGGALLWGLKCAKRKNMRRLVAKSDCLRLIQILGKKELCPVDFEPIFDQIMEMYGFFSSCQFMFVRRDDNVKAHCLAKHGLYLLSCSE
Homology
BLAST of Lag0028806 vs. NCBI nr
Match: PWA73504.1 (reverse transcriptase [Artemisia annua])

HSP 1 Score: 65.5 bits (158), Expect = 4.0e-07
Identity = 41/123 (33.33%), Postives = 62/123 (50.41%), Query Frame = 0

Query: 3   TDAAWMVGTLATGYGWHMEEHGGGGVSEGAQPGGKCLSSLHAEGGALLWGLKCAKRKNMR 62
           TDA+W   T   G G+    H G  +  GA+      S L AE  A++W +  A  KN +
Sbjct: 295 TDASWQKETGRAGLGFVARNHNGEVLLSGARVECYAASPLEAEAKAIMWAMTHALSKNFQ 354

Query: 63  RLVAKSDCLRLIQILGKKELCPVDFEPIFDQIMEMYGFFSSCQFMFVRRDDNVKAHCLAK 122
            +V +SD L L+  L  + +       +F QI+     FS+C + FV+R+ N+ AH +A 
Sbjct: 355 NVVFESDSLCLVNALRYRSVLR-QITCLFSQILVSSEAFSTCNWSFVKREGNMVAHSIAT 414

Query: 123 HGL 126
            GL
Sbjct: 415 WGL 416

BLAST of Lag0028806 vs. NCBI nr
Match: XP_024011123.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC112086436 [Eutrema salsugineum])

HSP 1 Score: 65.5 bits (158), Expect = 4.0e-07
Identity = 40/120 (33.33%), Postives = 65/120 (54.17%), Query Frame = 0

Query: 2    WTDAAWMVGTLATGYGWHMEEHGGGGVSEGAQPGGKCLSSLHAEGGALLWGLKCAKRKNM 61
            +TDA+W +G   +GYGW +E+ GG  +  G     +  S LH E G+L+W ++C  R ++
Sbjct: 1103 FTDASWKIGVPTSGYGWVLEQ-GGVLLHLGLHGVRRRQSPLHVELGSLIWAMECLIRLSL 1162

Query: 62   RRLVAKSDCLRLIQILGKKELCPVDFEPIFDQIMEMYGFFSSCQFMFVRRDDNVKAHCLA 121
            R +   +DCL L+ ++      P  F    D ++E+   FSS    +V R +N +A  LA
Sbjct: 1163 RAIAFATDCLDLVHMIENLSDWPT-FASELDDMIEIKKRFSSFSIRYVPRLENSRADFLA 1220

BLAST of Lag0028806 vs. NCBI nr
Match: KAF2550819.1 (hypothetical protein F2Q68_00035353, partial [Brassica cretica])

HSP 1 Score: 65.5 bits (158), Expect = 4.0e-07
Identity = 39/119 (32.77%), Postives = 60/119 (50.42%), Query Frame = 0

Query: 4   DAAWMVGTLATGYGWHMEEHGGGGVSEGAQPGGKCLSSLHAEGGALLWGLKCAKRKNMRR 63
           DA+W       G G+ ME   G  +  G+    + LS LHAE   LLW +K +       
Sbjct: 305 DASWKEDDARYGGGFAMENEDGNTMF-GSIASNRVLSPLHAEFATLLWAMKSSLSLGHVS 364

Query: 64  LVAKSDCLRLIQILGKKELCPVDFEPIFDQIMEMYGFFSSCQFMFVRRDDNVKAHCLAK 123
           +  +SDCL+L++++ ++E C       FD+ + +   F  C   FV R  N++A CLAK
Sbjct: 365 MAFESDCLQLVRLIEEEEEC-ASLVAEFDEFLNLRSMFQICSISFVSRLKNLRADCLAK 421

BLAST of Lag0028806 vs. NCBI nr
Match: XP_024016341.1 (uncharacterized protein LOC112089817 [Eutrema salsugineum])

HSP 1 Score: 65.1 bits (157), Expect = 5.2e-07
Identity = 41/123 (33.33%), Postives = 66/123 (53.66%), Query Frame = 0

Query: 4    DAAWMVGTLATGYGWHMEEHGGGGVSEGAQPGGKCLSSLHAEGGALLWGLKCAKRKNMRR 63
            DA+W+     +G+GW +E+ G GG   G Q   + LS+LHAE   LLW + C + + +  
Sbjct: 1108 DASWIDHGPVSGFGWTLED-GRGGEFFGQQGCPRSLSALHAEMNCLLWAMSCLRERQITS 1167

Query: 64   LVAKSDCLRLIQILGKKELCPVDFEPIFDQIMEMY----GFFSSCQFMFVRRDDNVKAHC 123
            ++ ++DCL L+ +       P D+ PIF   +E++    G FS      + R  N++A  
Sbjct: 1168 VLFQTDCLDLVSM----SESPADW-PIFRSELEIFGNLRGSFSEFALSHIPRSQNIRADS 1224

BLAST of Lag0028806 vs. NCBI nr
Match: XP_010512831.1 (PREDICTED: uncharacterized protein LOC104788746 [Camelina sativa])

HSP 1 Score: 64.3 bits (155), Expect = 8.9e-07
Identity = 42/123 (34.15%), Postives = 63/123 (51.22%), Query Frame = 0

Query: 3   TDAAWMVGTLATGYGWHMEEHGGGGVSEGAQPGGKCLSSLHAEGGALLWGLKCAKRKNMR 62
           +DAAW     A G GW  + H G  V +G+ P     + L AEG A+L  +  A    + 
Sbjct: 266 SDAAWNTSKQA-GLGWISKNHRGITVDQGSTPVSNVHTPLVAEGLAMLQAVTSASNLGLT 325

Query: 63  RLVAKSDCLRLIQILGKKELCPVDFEPIFDQIMEMYGFFSSCQFMFVRRDDNVKAHCLAK 122
            L+  SD + L++ + K E  P +   I   I+++   FS   F F+ R +NV+A CLAK
Sbjct: 326 HLIFASDSINLVKAI-KSENQPKELHGILCDILKISQSFSHVSFRFIPRSNNVEADCLAK 385

Query: 123 HGL 126
           + L
Sbjct: 386 NAL 386

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PWA73504.14.0e-0733.33reverse transcriptase [Artemisia annua][more]
XP_024011123.14.0e-0733.33LOW QUALITY PROTEIN: uncharacterized protein LOC112086436 [Eutrema salsugineum][more]
KAF2550819.14.0e-0732.77hypothetical protein F2Q68_00035353, partial [Brassica cretica][more]
XP_024016341.15.2e-0733.33uncharacterized protein LOC112089817 [Eutrema salsugineum][more]
XP_010512831.18.9e-0734.15PREDICTED: uncharacterized protein LOC104788746 [Camelina sativa][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 3..123
e-value: 2.1E-20
score: 72.8
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..127
e-value: 1.6E-11
score: 46.3
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 3..122
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 3..122
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 3..122
e-value: 3.05306E-14
score: 62.3316
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 2..126

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0028806.1Lag0028806.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity