Lag0028788 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0028788
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionUlp1-like peptidase
Locationchr8: 30726921 .. 30728754 (+)
RNA-Seq ExpressionLag0028788
SyntenyLag0028788
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATAGCCAGATGGAGAGAATGGATGCTCACATGTCTGATATGGAGACATGCCTCAAGTCTATTACCAAGTTCCTACGTCGTCTCTCTAAAGTATTTTGGTAGTTGAACATTGTTATTGTATTGGTTGGTTTTATTTCTTCTAACATTTGTAAACTTTCAGGGTAAATTCGTGGACCCTGAGAAGTACTTTGGACCAAAAGATGGTCTGGATGGTGAGGGTGGTCCATCAAAAGGACCAGATGACGTGGGTGGTCCATCGAAAGGACCCGATGACAAGGGTGGTCCATCGAAAGGACCGGATGAAAAGGGTAGATCGGATGACAATAAGGAAGAAAGACATGAAGGAGGAGGAAGCGGCAGAGGAGAGGACGAGAAGGAGAAGGACGTTGGTGAGACGCACGACATAGAGCATATTACGGAAATAATATTTCTTACCCTATCATATTTTAAATTTAACATTTCCATATATAAGTGTAATTTGATATACAGCAGTTGAAGTCTCAACAACCACTGATGTAGAGTCTCACTCCATTACTGACGTGGAGTCTCAACCAACCACTGACGTAAAGTCTCACTCCATTACTAACGTGAAGTCTCAACCAATTACAGACCCAGTCGAGTTCATTGTACCTAAGGTTGAGGTAATTATTTGTCTTAATGAAAACAATATTATATAAATCTCACTAATGTATAAAATGTATGTTTGTAGCCTGTTGAGTTAAGTGATGAGCAGGTTGAGGAGGTTAAAGTAATCGGGCCGCAGGAATTGGTGAAAAGACGGGGAAAGCGGACCCGACAAATTTCTTGGAAGCTTCGGTCTCCATGGGCTGACACCAGGCCGGACGACAAAAGGAGGAAAGTTAAGCAATACGATCCCATGCGTGCCATTCCTAAGGAGTACGAGACCACGTTTCAGAAATGGTTGGATGACCCATTGTCTGATGGATCAGAGCGCAAGACAGTATATGCATACAGAAGCAAACAATGGTTCAAGACGTTACTCACATCGTCTCATTGGATGAGTGATGAGGTACAACCTATTTTATCACTGCTGTTGATTTGTAAATCTTATGGTTTTAGACATAGGGTTTATAGTTAAAGAGGACTTAGAGTTTCTTCTGATACTATAGGTGATTGACTCTCTCTTCCTCTTTGTTCAAAGAAGATGGATATCCGTCCTGACTTATGCCATAGAAAGTTTGTCACGGCGGATGTATTTGTAACAGTACATATTTTCATCTTATAGAATTTCACTTAATCTGAATTGTACTAATATTGGATGTTGTGCAGGAATTTTTGAGGCGCGGGGATGTGTACGAAGAACTCCTTCGTAGTGACCATGGAACGACACGTTCGATTGGAGTAGATTCAAGATGGTCACTAACTACGTAATGGGAGAACACACAGATTAAAATGTTCATTGGAGTTCAGTTGATGTCTACATGCCCTTCAACTTAGGTAGAAACCATTGGCTTCTACTGTGCGCTGACTTTGAAACGGGCGAATTTGTGTTGACAGACTCCCTAACGGCACTGAATTCAGATGCAGACATAGCAAAGTAGATGAATACGGTATGCACCATTTTCCCAAGGTTGCTACTGAGGTGCGACGTTATGAAGGAGAAGCCGTCTCTTCCAACACATCCACGACGATTCAGAAGGAAGACCCAATGCCACAACAACAAGATAGTGGGGATTGTGGGGTTTTCACTATAATTTTTTTGGAATATGATGTAACTAGATCAGATTTAGGTAGTCTTAGTCAAGAGAAAATGGAGTTTTGTAGGCGTCAATTTGATGTACAACTGTGGGCCAATAGGTCGTTCTTTTAG

mRNA sequence

ATGGATAGCCAGATGGAGAGAATGGATGCTCACATGTCTGATATGGAGACATGCCTCAAGTCTATTACCAAGTTCCTACGTCGTCTCTCTAAAGGTAAATTCGTGGACCCTGAGAAGTACTTTGGACCAAAAGATGGTCTGGATGGTGAGGGTGGTCCATCAAAAGGACCAGATGACGTGGGTGGTCCATCGAAAGGACCCGATGACAAGGGTGGTCCATCGAAAGGACCGGATGAAAAGGGTAGATCGGATGACAATAAGGAAGAAAGACATGAAGGAGGAGGAAGCGGCAGAGGAGAGGACGAGAAGGAGAAGGACGTTGCAGTTGAAGTCTCAACAACCACTGATGTAGAGTCTCACTCCATTACTGACGTGGAGTCTCAACCAACCACTGACGTAAAGTCTCACTCCATTACTAACGTGAAGTCTCAACCAATTACAGACCCAGTCGAGTTCATTGTACCTAAGGTTGAGCCTGTTGAGTTAAGTGATGAGCAGGTTGAGGAGGTTAAAGTAATCGGGCCGCAGGAATTGGTGAAAAGACGGGGAAAGCGGACCCGACAAATTTCTTGGAAGCTTCGGTCTCCATGGGCTGACACCAGGCCGGACGACAAAAGGAGGAAAGTTAAGCAATACGATCCCATGCGTGCCATTCCTAAGGAGTACGAGACCACGTTTCAGAAATGGTTGGATGACCCATTGTCTGATGGATCAGAGCGCAAGACAGTATATGCATACAGAAGCAAACAATGGTTCAAGACGTTACTCACATCGTCTCATTGGATGAGTGATGAGGAATTTTTGAGGCGCGGGGATGTGTACGAAGAACTCCTTCGTAGTGACCATGGAACGACACGTTCGATTGGAGTAGATTCAAGATGGTCACTAACTACACTCCCTAACGGCACTGAATTCAGATGCAGACATAGCAAAGTAGATGAATACGGTATGCACCATTTTCCCAAGGTTGCTACTGAGGTGCGACGTTATGAAGGAGAAGCCGTCTCTTCCAACACATCCACGACGATTCAGAAGGAAGACCCAATGCCACAACAACAAGATAGTGGGGATTGTGGGGTTTTCACTATAATTTTTTTGGAATATGATGTAACTAGATCAGATTTAGGTAGTCTTAGTCAAGAGAAAATGGAGTTTTGTAGGCGTCAATTTGATGTACAACTGTGGGCCAATAGGTCGTTCTTTTAG

Coding sequence (CDS)

ATGGATAGCCAGATGGAGAGAATGGATGCTCACATGTCTGATATGGAGACATGCCTCAAGTCTATTACCAAGTTCCTACGTCGTCTCTCTAAAGGTAAATTCGTGGACCCTGAGAAGTACTTTGGACCAAAAGATGGTCTGGATGGTGAGGGTGGTCCATCAAAAGGACCAGATGACGTGGGTGGTCCATCGAAAGGACCCGATGACAAGGGTGGTCCATCGAAAGGACCGGATGAAAAGGGTAGATCGGATGACAATAAGGAAGAAAGACATGAAGGAGGAGGAAGCGGCAGAGGAGAGGACGAGAAGGAGAAGGACGTTGCAGTTGAAGTCTCAACAACCACTGATGTAGAGTCTCACTCCATTACTGACGTGGAGTCTCAACCAACCACTGACGTAAAGTCTCACTCCATTACTAACGTGAAGTCTCAACCAATTACAGACCCAGTCGAGTTCATTGTACCTAAGGTTGAGCCTGTTGAGTTAAGTGATGAGCAGGTTGAGGAGGTTAAAGTAATCGGGCCGCAGGAATTGGTGAAAAGACGGGGAAAGCGGACCCGACAAATTTCTTGGAAGCTTCGGTCTCCATGGGCTGACACCAGGCCGGACGACAAAAGGAGGAAAGTTAAGCAATACGATCCCATGCGTGCCATTCCTAAGGAGTACGAGACCACGTTTCAGAAATGGTTGGATGACCCATTGTCTGATGGATCAGAGCGCAAGACAGTATATGCATACAGAAGCAAACAATGGTTCAAGACGTTACTCACATCGTCTCATTGGATGAGTGATGAGGAATTTTTGAGGCGCGGGGATGTGTACGAAGAACTCCTTCGTAGTGACCATGGAACGACACGTTCGATTGGAGTAGATTCAAGATGGTCACTAACTACACTCCCTAACGGCACTGAATTCAGATGCAGACATAGCAAAGTAGATGAATACGGTATGCACCATTTTCCCAAGGTTGCTACTGAGGTGCGACGTTATGAAGGAGAAGCCGTCTCTTCCAACACATCCACGACGATTCAGAAGGAAGACCCAATGCCACAACAACAAGATAGTGGGGATTGTGGGGTTTTCACTATAATTTTTTTGGAATATGATGTAACTAGATCAGATTTAGGTAGTCTTAGTCAAGAGAAAATGGAGTTTTGTAGGCGTCAATTTGATGTACAACTGTGGGCCAATAGGTCGTTCTTTTAG

Protein sequence

MDSQMERMDAHMSDMETCLKSITKFLRRLSKGKFVDPEKYFGPKDGLDGEGGPSKGPDDVGGPSKGPDDKGGPSKGPDEKGRSDDNKEERHEGGGSGRGEDEKEKDVAVEVSTTTDVESHSITDVESQPTTDVKSHSITNVKSQPITDPVEFIVPKVEPVELSDEQVEEVKVIGPQELVKRRGKRTRQISWKLRSPWADTRPDDKRRKVKQYDPMRAIPKEYETTFQKWLDDPLSDGSERKTVYAYRSKQWFKTLLTSSHWMSDEEFLRRGDVYEELLRSDHGTTRSIGVDSRWSLTTLPNGTEFRCRHSKVDEYGMHHFPKVATEVRRYEGEAVSSNTSTTIQKEDPMPQQQDSGDCGVFTIIFLEYDVTRSDLGSLSQEKMEFCRRQFDVQLWANRSFF
Homology
BLAST of Lag0028788 vs. NCBI nr
Match: XP_038882332.1 (uncharacterized protein LOC120073583 [Benincasa hispida])

HSP 1 Score: 71.6 bits (174), Expect = 1.7e-08
Identity = 29/60 (48.33%), Postives = 46/60 (76.67%), Query Frame = 0

Query: 342 TIQKEDPMPQQQDSGDCGVFTIIFLEYDVTRSDLGSLSQEKMEFCRRQFDVQLWANRSFF 401
           T++++    QQ +SGDCG+FT  F EYDVT S +G+L+Q++ ++ RRQ+ +Q+WANR+ F
Sbjct: 159 TLRRDAFRSQQHESGDCGMFTYKFFEYDVTGSKMGTLTQDRKQYFRRQYAIQIWANRALF 218

BLAST of Lag0028788 vs. NCBI nr
Match: XP_038885861.1 (sentrin-specific protease [Benincasa hispida])

HSP 1 Score: 68.6 bits (166), Expect = 1.4e-07
Identity = 29/60 (48.33%), Postives = 46/60 (76.67%), Query Frame = 0

Query: 342 TIQKEDPMPQQQDSGDCGVFTIIFLEYDVTRSDLGSLSQEKMEFCRRQFDVQLWANRSFF 401
           T++++  +PQQ  SGDCG+FT  F EYDVT S + +L+Q++M++ RRQ+ +Q+ ANR+ F
Sbjct: 130 TLRRDAFVPQQDKSGDCGMFTCKFFEYDVTGSKMDTLTQDRMQYYRRQYAIQISANRTLF 189

BLAST of Lag0028788 vs. NCBI nr
Match: XP_022154364.1 (uncharacterized protein LOC111021646 [Momordica charantia])

HSP 1 Score: 68.2 bits (165), Expect = 1.9e-07
Identity = 32/61 (52.46%), Postives = 39/61 (63.93%), Query Frame = 0

Query: 339 TSTTIQKEDPMPQQQDSGDCGVFTIIFLEYDVTRSDLGSLSQEKMEFCRRQFDVQLWANR 398
           T   I++    PQQ   GDCG+F I F EYDVT     +L+Q +M F RRQF VQLWAN+
Sbjct: 151 TPWRIRRVSSAPQQGMDGDCGIFCINFFEYDVTSCSFDTLTQSRMSFFRRQFAVQLWANK 210

Query: 399 S 400
           S
Sbjct: 211 S 211

BLAST of Lag0028788 vs. NCBI nr
Match: XP_022159362.1 (uncharacterized protein LOC111025779 [Momordica charantia])

HSP 1 Score: 67.4 bits (163), Expect = 3.2e-07
Identity = 27/52 (51.92%), Postives = 39/52 (75.00%), Query Frame = 0

Query: 350 PQQQDSGDCGVFTIIFLEYDVTRSDLGSLSQEKMEFCRRQFDVQLWANRSFF 402
           PQQ  SGDCG+F + + EYDVTRS++ SL+Q+ + F R +  +++WANRS F
Sbjct: 97  PQQSGSGDCGMFCVKYFEYDVTRSNMTSLTQDNISFFREKLAIEMWANRSIF 148

BLAST of Lag0028788 vs. NCBI nr
Match: RVW59689.1 (hypothetical protein CK203_101032 [Vitis vinifera])

HSP 1 Score: 63.9 bits (154), Expect = 3.6e-06
Identity = 64/255 (25.10%), Postives = 109/255 (42.75%), Query Frame = 0

Query: 161 ELSDEQVEEVKVIGPQELVKRRGKRTRQISWKLRSPWADTRPDDKRRK-----VKQYDPM 220
           EL D+   +  VI   ++ K  G  T  IS +   P+ D     K RK     +  +DP+
Sbjct: 53  ELKDDYSNDDSVI---DIAKLFG--TPTISDEFSHPFTDPTKRKKLRKEIEEPLTLFDPL 112

Query: 221 RAIPKEYETTFQKWLDDPLSDGSERKTVYAYRSKQWFKTLLTSSHWMSDEE------FLR 280
           R   +E   +F+KW+ +    GS     Y +  K+WF++L     W+ D        F R
Sbjct: 113 RPTSEEALESFRKWMSN--DQGSTIDIDYMHIDKKWFQSLSNHGSWLPDTHINVAFFFFR 172

Query: 281 RGDVYEELLRSDHGTT-----------RSIGVDSRWSLTTLP---------NGTEFRCRH 340
           +  +    + S   TT           R I  D +W+   LP         NG++    +
Sbjct: 173 KRRIENPHMFSQKLTTMDIMFWQNARVRWIKYDKKWNQFKLPENDILIDYANGSQ-PLYY 232

Query: 341 SKVDEYGMHHFPKVATEVRRYEGEAVSSNTSTTIQKEDPMPQQQDSGDCGVFTIIFLEYD 385
            K  +  + H P +   V  + G+   +     I++   +PQQ+ +GDC +F I ++EY 
Sbjct: 233 VKWTDVDIVHVPSMFGLVIGFYGDTKVNYYEWEIERLQDIPQQEHAGDCDMFIIKYVEYL 292

BLAST of Lag0028788 vs. ExPASy TrEMBL
Match: A0A6J1DLV0 (uncharacterized protein LOC111021646 OS=Momordica charantia OX=3673 GN=LOC111021646 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 9.1e-08
Identity = 32/61 (52.46%), Postives = 39/61 (63.93%), Query Frame = 0

Query: 339 TSTTIQKEDPMPQQQDSGDCGVFTIIFLEYDVTRSDLGSLSQEKMEFCRRQFDVQLWANR 398
           T   I++    PQQ   GDCG+F I F EYDVT     +L+Q +M F RRQF VQLWAN+
Sbjct: 151 TPWRIRRVSSAPQQGMDGDCGIFCINFFEYDVTSCSFDTLTQSRMSFFRRQFAVQLWANK 210

Query: 399 S 400
           S
Sbjct: 211 S 211

BLAST of Lag0028788 vs. ExPASy TrEMBL
Match: A0A6J1DZM8 (uncharacterized protein LOC111025779 OS=Momordica charantia OX=3673 GN=LOC111025779 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.6e-07
Identity = 27/52 (51.92%), Postives = 39/52 (75.00%), Query Frame = 0

Query: 350 PQQQDSGDCGVFTIIFLEYDVTRSDLGSLSQEKMEFCRRQFDVQLWANRSFF 402
           PQQ  SGDCG+F + + EYDVTRS++ SL+Q+ + F R +  +++WANRS F
Sbjct: 97  PQQSGSGDCGMFCVKYFEYDVTRSNMTSLTQDNISFFREKLAIEMWANRSIF 148

BLAST of Lag0028788 vs. ExPASy TrEMBL
Match: A0A438FI83 (ULP_PROTEASE domain-containing protein OS=Vitis vinifera OX=29760 GN=CK203_101032 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.7e-06
Identity = 64/255 (25.10%), Postives = 109/255 (42.75%), Query Frame = 0

Query: 161 ELSDEQVEEVKVIGPQELVKRRGKRTRQISWKLRSPWADTRPDDKRRK-----VKQYDPM 220
           EL D+   +  VI   ++ K  G  T  IS +   P+ D     K RK     +  +DP+
Sbjct: 53  ELKDDYSNDDSVI---DIAKLFG--TPTISDEFSHPFTDPTKRKKLRKEIEEPLTLFDPL 112

Query: 221 RAIPKEYETTFQKWLDDPLSDGSERKTVYAYRSKQWFKTLLTSSHWMSDEE------FLR 280
           R   +E   +F+KW+ +    GS     Y +  K+WF++L     W+ D        F R
Sbjct: 113 RPTSEEALESFRKWMSN--DQGSTIDIDYMHIDKKWFQSLSNHGSWLPDTHINVAFFFFR 172

Query: 281 RGDVYEELLRSDHGTT-----------RSIGVDSRWSLTTLP---------NGTEFRCRH 340
           +  +    + S   TT           R I  D +W+   LP         NG++    +
Sbjct: 173 KRRIENPHMFSQKLTTMDIMFWQNARVRWIKYDKKWNQFKLPENDILIDYANGSQ-PLYY 232

Query: 341 SKVDEYGMHHFPKVATEVRRYEGEAVSSNTSTTIQKEDPMPQQQDSGDCGVFTIIFLEYD 385
            K  +  + H P +   V  + G+   +     I++   +PQQ+ +GDC +F I ++EY 
Sbjct: 233 VKWTDVDIVHVPSMFGLVIGFYGDTKVNYYEWEIERLQDIPQQEHAGDCDMFIIKYVEYL 292

BLAST of Lag0028788 vs. ExPASy TrEMBL
Match: A0A438FU99 (Ubiquitin-like-specific protease ESD4 OS=Vitis vinifera OX=29760 GN=ESD4_4 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 5.0e-06
Identity = 50/211 (23.70%), Postives = 95/211 (45.02%), Query Frame = 0

Query: 205 KRRKVKQ--------YDPMRAIPKEYETTFQKWLDDPLSDGSERKTVYAYRSKQWFKTLL 264
           KR+K+++        +DP+  I +E   +FQKW+ D    GS     Y +  K+WF++L 
Sbjct: 84  KRKKLRKEIEKPLTSFDPLHPISEEALESFQKWMSD--DQGSTIDIDYMHIDKKWFQSLS 143

Query: 265 TSSHWMSDE------EFLRRGDVYEEL-LRSDHGTTRSIGVDSRW-----SLTTLPNGTE 324
               W++D       ++     VY  + +R+ H     + +  R      SL  + N   
Sbjct: 144 NHGSWLADTVSSYSVKWPDVDIVYVPINVRASHWVLGVVHLHRRIIYVYDSLMGINNNAR 203

Query: 325 FRCRHSKVDEYGMHHFPKVATEVRRYEGEAVSSNTSTTIQKEDPMPQQQDSGDCGVFTII 384
            +     + +   H    +A     + G+   +     I++   +PQQ++ GDCG+F I 
Sbjct: 204 LQVAIKPLAKLLPHILDAIA--YYGFHGDTKVNYQEWEIERLQDIPQQENDGDCGMFVIK 263

Query: 385 FLEYDVTRSDLGSLSQEKMEFCRRQFDVQLW 396
           + EY +    L SL+  +M++ R +   +L+
Sbjct: 264 YAEYLMHNHPLKSLTSARMDWFREKMAAELF 290

BLAST of Lag0028788 vs. ExPASy TrEMBL
Match: A0A6J1DQZ3 (uncharacterized protein LOC111023442 OS=Momordica charantia OX=3673 GN=LOC111023442 PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 8.5e-06
Identity = 27/61 (44.26%), Postives = 39/61 (63.93%), Query Frame = 0

Query: 339 TSTTIQKEDPMPQQQDSGDCGVFTIIFLEYDVTRSDLGSLSQEKMEFCRRQFDVQLWANR 398
           T   I++    P+Q  SGDCG+F + + EYDVT + L +L Q  M + RRQF  QLW+N+
Sbjct: 89  TPWRIRRVTSTPEQFSSGDCGIFCVKYFEYDVTWTSLETLRQNNMSYFRRQFAFQLWSNK 148

Query: 399 S 400
           +
Sbjct: 149 A 149

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038882332.11.7e-0848.33uncharacterized protein LOC120073583 [Benincasa hispida][more]
XP_038885861.11.4e-0748.33sentrin-specific protease [Benincasa hispida][more]
XP_022154364.11.9e-0752.46uncharacterized protein LOC111021646 [Momordica charantia][more]
XP_022159362.13.2e-0751.92uncharacterized protein LOC111025779 [Momordica charantia][more]
RVW59689.13.6e-0625.10hypothetical protein CK203_101032 [Vitis vinifera][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DLV09.1e-0852.46uncharacterized protein LOC111021646 OS=Momordica charantia OX=3673 GN=LOC111021... [more]
A0A6J1DZM81.6e-0751.92uncharacterized protein LOC111025779 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A438FI831.7e-0625.10ULP_PROTEASE domain-containing protein OS=Vitis vinifera OX=29760 GN=CK203_10103... [more]
A0A438FU995.0e-0623.70Ubiquitin-like-specific protease ESD4 OS=Vitis vinifera OX=29760 GN=ESD4_4 PE=3 ... [more]
A0A6J1DQZ38.5e-0644.26uncharacterized protein LOC111023442 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003653Ulp1 protease family, C-terminal catalytic domainPFAMPF02902Peptidase_C48coord: 338..392
e-value: 2.9E-5
score: 24.0
NoneNo IPR availableGENE3D3.40.395.10Adenoviral Proteinase; Chain Acoord: 292..398
e-value: 5.4E-8
score: 34.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 71..111
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..135
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 112..135
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 340..396

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0028788.1Lag0028788.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008234 cysteine-type peptidase activity