Lag0028773 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0028773
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRibonuclease H
Locationchr8: 30493085 .. 30493591 (-)
RNA-Seq ExpressionLag0028773
SyntenyLag0028773
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAGTAATGGAGTGGACACAACGTTGTGAATGGATCCAATGTTATTGGCTCGAAACTCGACCTACAGTTGTGGAGATTATGGGGCGAAATGGATTAGAAGATGATAGACATGTTAGTCCTATTGCTTGCCACGTATATACGGATGTTGCGGTGAATAGGAACAATGATGGAACTGGTTTAGGAGTAGTTGTGCTTCAACCGGATGGGTTCCTTCTCGCTGCCATGGAGATGGAGAATAAAATTGGGTACTCACCTTTGGCAGCAGAGTGCCAAGCCATTAGCAAAGGCATCAGTCTATTACAACGGTTACAAATTACAGATGTTTGCCTTTACTCTGACTCTAGCAATGCTATTCAAATGATTATGGGGGAGCATAGCATTACTACGAATGTGTATCAATGGGTGATCCAAACTCAGGCTCTTGCAAAGGATTTTGCTTCGTTAAATTTTGTTCATATTTCTAGAACTAGAAATAGGCGAGCTGATTATTTAGGGGCCGTTTGA

mRNA sequence

ATGCCAGTAATGGAGTGGACACAACGTTGTGAATGGATCCAATGTTATTGGCTCGAAACTCGACCTACAGTTGTGGAGATTATGGGGCGAAATGGATTAGAAGATGATAGACATGTTAGTCCTATTGCTTGCCACGTATATACGGATGTTGCGGTGAATAGGAACAATGATGGAACTGGTTTAGGAGTAGTTGTGCTTCAACCGGATGGGTTCCTTCTCGCTGCCATGGAGATGGAGAATAAAATTGGGTACTCACCTTTGGCAGCAGAGTGCCAAGCCATTAGCAAAGGCATCAGTCTATTACAACGGTTACAAATTACAGATGTTTGCCTTTACTCTGACTCTAGCAATGCTATTCAAATGATTATGGGGGAGCATAGCATTACTACGAATGTGTATCAATGGGTGATCCAAACTCAGGCTCTTGCAAAGGATTTTGCTTCGTTAAATTTTGTTCATATTTCTAGAACTAGAAATAGGCGAGCTGATTATTTAGGGGCCGTTTGA

Coding sequence (CDS)

ATGCCAGTAATGGAGTGGACACAACGTTGTGAATGGATCCAATGTTATTGGCTCGAAACTCGACCTACAGTTGTGGAGATTATGGGGCGAAATGGATTAGAAGATGATAGACATGTTAGTCCTATTGCTTGCCACGTATATACGGATGTTGCGGTGAATAGGAACAATGATGGAACTGGTTTAGGAGTAGTTGTGCTTCAACCGGATGGGTTCCTTCTCGCTGCCATGGAGATGGAGAATAAAATTGGGTACTCACCTTTGGCAGCAGAGTGCCAAGCCATTAGCAAAGGCATCAGTCTATTACAACGGTTACAAATTACAGATGTTTGCCTTTACTCTGACTCTAGCAATGCTATTCAAATGATTATGGGGGAGCATAGCATTACTACGAATGTGTATCAATGGGTGATCCAAACTCAGGCTCTTGCAAAGGATTTTGCTTCGTTAAATTTTGTTCATATTTCTAGAACTAGAAATAGGCGAGCTGATTATTTAGGGGCCGTTTGA

Protein sequence

MPVMEWTQRCEWIQCYWLETRPTVVEIMGRNGLEDDRHVSPIACHVYTDVAVNRNNDGTGLGVVVLQPDGFLLAAMEMENKIGYSPLAAECQAISKGISLLQRLQITDVCLYSDSSNAIQMIMGEHSITTNVYQWVIQTQALAKDFASLNFVHISRTRNRRADYLGAV
Homology
BLAST of Lag0028773 vs. NCBI nr
Match: XP_030926327.1 (uncharacterized protein LOC115953035, partial [Quercus lobata])

HSP 1 Score: 70.9 bits (172), Expect = 1.2e-08
Identity = 43/126 (34.13%), Postives = 75/126 (59.52%), Query Frame = 0

Query: 46  VYTDVAVNRNNDGTGLGVVVLQPDGFLLAAMEMENKIGYSPL--AAECQAISKGISLLQR 105
           VY D A N+   G+G+G+V++ PDG   AA+E   ++G+S     AE +A+ +G++++QR
Sbjct: 512 VYVDGAANQR--GSGVGIVLISPDG---AAIEKSLRLGFSATNNEAEYEALLQGMTMVQR 571

Query: 106 LQITDVCLYSDSSNAIQMIMGE-HSITTNVYQWVIQTQALAKDFASLNFVHISRTRNRRA 165
           +    +  +SDS   ++ +MGE  +  T + +++ Q + L  +F S N  HISR+ N  A
Sbjct: 572 MGGKMIEAFSDSRLVVRQVMGELEARDTRMQEYLGQVKRLQMNFESFNLTHISRSGNTHA 631

Query: 166 DYLGAV 169
           D L  +
Sbjct: 632 DSLATL 632

BLAST of Lag0028773 vs. NCBI nr
Match: XP_016199385.1 (uncharacterized protein LOC107640375 [Arachis ipaensis])

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-06
Identity = 37/135 (27.41%), Postives = 68/135 (50.37%), Query Frame = 0

Query: 33  LEDDRHVSPIACHVYTDVAVNRNNDGTGLGVVVLQPDGFLLAAMEMENKIGYSPLAAECQ 92
           + +D H + +   ++ D A N+   G G   ++L+    ++A   ++ +   S   AE +
Sbjct: 267 MTNDTHNTEVRWSIHVDGASNKEGSGAG---ILLKEGNKVVAEQSLQFRFNASNNQAEYE 326

Query: 93  AISKGISLLQRLQITDVCLYSDSSNAIQMIMGEHSITTNVYQ--WVIQTQALAKDFASLN 152
           A+  G+ L  RLQI  +  Y DSS  +  I GE  +   + +  W++ T+ L   F   +
Sbjct: 327 ALLAGLKLTLRLQIPRITAYCDSSLVVHQIKGEFQVKDPLLEKYWLV-TKDLISKFKEFD 386

Query: 153 FVHISRTRNRRADYL 166
            +H++R +N RAD L
Sbjct: 387 IIHVNREQNTRADVL 397

BLAST of Lag0028773 vs. NCBI nr
Match: XP_022154440.1 (uncharacterized protein LOC111021711 [Momordica charantia])

HSP 1 Score: 63.2 bits (152), Expect = 2.5e-06
Identity = 39/118 (33.05%), Postives = 58/118 (49.15%), Query Frame = 0

Query: 40  SPIACHVYTDVAVNRNNDGTGLGVVVLQPDGFLLAAMEMENKIGYSPLAAECQAISKGIS 99
           SP    +  D A   +N  TG+GVV     G ++ AM  +  + Y PL  E  AI +G+ 
Sbjct: 132 SPDWVKINVDTACKLSNSRTGIGVVCQNDKGEIIVAMIRKLDVFYEPLQVETLAILEGLW 191

Query: 100 LLQRLQITDVCLYSDSSNAIQMIMGEHSITTNVYQWVIQTQALAKDFASLNFVHISRT 158
           L Q L +  V + SDS N+I +I  E+  +     WV      A +F  + F+HI R+
Sbjct: 192 LTQCLDLIFVQVDSDSQNSINLISQEYDPSHQPNVWVDDIHMCAINFIDILFMHIQRS 249

BLAST of Lag0028773 vs. NCBI nr
Match: XP_030939953.1 (uncharacterized protein LOC115964855 [Quercus lobata])

HSP 1 Score: 62.8 bits (151), Expect = 3.3e-06
Identity = 37/131 (28.24%), Postives = 72/131 (54.96%), Query Frame = 0

Query: 41  PIACHVYTDVAVNRNNDGTGLGVVVLQPDGFLLAAMEMENKIGYSPL--AAECQAISKGI 100
           P     Y D A N+   G G+G+V++ P+G     +E   ++G+S     AE +A+ +G+
Sbjct: 286 PTCWKAYIDSATNQR--GFGVGLVLISPEGI---TIEKSPRLGFSATNNEAEYEALLEGM 345

Query: 101 SLLQRLQITDVCLYSDSSNAIQMIMGE-HSITTNVYQWVIQTQALAKDFASLNFVHISRT 160
           S++++L    + ++SDS   +  + GE  +    + ++++Q + L   F S + +H+SR+
Sbjct: 346 SMVEKLGGKSINMFSDSRPVVGQVNGELEAKDERMQEYLVQAKHLRTRFNSFSLMHVSRS 405

Query: 161 RNRRADYLGAV 169
           RN  AD L  +
Sbjct: 406 RNTHADSLATL 411

BLAST of Lag0028773 vs. NCBI nr
Match: XP_022158489.1 (uncharacterized protein LOC111024968 [Momordica charantia])

HSP 1 Score: 62.0 bits (149), Expect = 5.7e-06
Identity = 47/160 (29.38%), Postives = 79/160 (49.38%), Query Frame = 0

Query: 9   RCEWIQCYWLETRPTVVEIMGRNGLEDDRHVSPI--ACHVYTDVAVNRNNDGTGLGVVVL 68
           + EWI  Y  E R      M R     +    P+     + TD AV  + +G+G+GV++ 
Sbjct: 38  KVEWILKYAEEVRMKYHNGMKRRIPRVNEWRPPLGGVVKMNTDAAV--SEEGSGVGVLLR 97

Query: 69  QPDGFLLAAMEMENKIGYSPLAAECQAISKGISLLQRLQITDVCLYSDSSNAIQMIMGEH 128
           + +  ++ AM ++  +  +PL A+  AI +G+SL  RL +  V + +DS  A+ +I  + 
Sbjct: 98  EGNAEIVGAM-VDFHMVKTPLLAKILAIREGLSLATRLGVHRVVVETDSLEALNLIGDKS 157

Query: 129 SITTNVYQWVIQTQALAKDFASLNFVHISRTRNRRADYLG 167
                   WV   +A A+DF  + F H+ R  N  A +LG
Sbjct: 158 PWKGEAVSWVEDIRAFARDFQEICFQHVFRESNAVAYHLG 194

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_030926327.11.2e-0834.13uncharacterized protein LOC115953035, partial [Quercus lobata][more]
XP_016199385.11.5e-0627.41uncharacterized protein LOC107640375 [Arachis ipaensis][more]
XP_022154440.12.5e-0633.05uncharacterized protein LOC111021711 [Momordica charantia][more]
XP_030939953.13.3e-0628.24uncharacterized protein LOC115964855 [Quercus lobata][more]
XP_022158489.15.7e-0629.38uncharacterized protein LOC111024968 [Momordica charantia][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 43..167
e-value: 8.6E-17
score: 63.4
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 48..165
e-value: 1.5E-19
score: 70.0
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 46..165
e-value: 2.53782E-17
score: 71.5764
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 45..165

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0028773.1Lag0028773.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity