Lag0028601 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0028601
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
Locationchr8: 26086629 .. 26087458 (-)
RNA-Seq ExpressionLag0028601
SyntenyLag0028601
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGCGAAAGTGGAATTGGAGAAATTCGACTGGAAGGGTGACTTCGGGCTATGGAGGAGAAAGATGAAAGCTCTTCTAGTCCAACAGAAGGTTGCTAACTTCTTGAACCAGGTAAGTTACCTACATCCTTGACTAAAGATGAGAAAGATGAAATGTGTGGGATTGCATATAGTTCTATAATTCTTCACCTCTCAGATAATGTTCATTGTAGGGTTAGTAAAATTGAAATAGTCGATGAATTATTGTGGTCTAAACTTGAAGAACTTTATTTGCTCAAAACATTAACTAATCAAAATTTCTTAAAGGAACATTTCTTCACCTATAAAATGGATTCGAACAAAAACCTTGAGGAAAATCTTGATGATTTTAATACAACTTGTACTGAATTAGCTAATGCCGGTGAAGAAATAAAATCTCAAGATCATGTTGTAATCCTCTTAAAGTCTTTGCTCGAATCTTATAAAGAAGTAAAAGTCGCTATAAAGTATGGTAGAGACAAATTGACACTCAATCAAATGCTTGACTCCTTGAGACTTAGAGAACTTGAGATAAAGTCTGAAAGGAAAGATAATGAAGTCTTGTTTGTTAGAGATAAGCAAAAGCATAACAAAAATAGAAATTCCTATAACAAGAATATTGGAAACCAAGCTTCAGGAAACCAAACCTACAGAAATAATAATCAAAATCAACACCACAATTCTAACCAAAACAGAGGCCAGAAGAGTTTCTGGAGAGACAATAGGAACTCGAATAAATCCTATCAACCTGCAAGAACTAATGAATCAACACAAATAAGTTGTAACTACTGTCACAAAGAAGGGCATTAA

mRNA sequence

ATGTCTGCGAAAGTGGAATTGGAGAAATTCGACTGGAAGGGTGACTTCGGGCTATGGAGGAGAAAGATGAAAGCTCTTCTAGTCCAACAGAAGGTTGCTAACTTCTTGAACCAGGAACATTTCTTCACCTATAAAATGGATTCGAACAAAAACCTTGAGGAAAATCTTGATGATTTTAATACAACTTGTACTGAATTAGCTAATGCCGGTGAAGAAATAAAATCTCAAGATCATGTTGTAATCCTCTTAAAGTCTTTGCTCGAATCTTATAAAGAAGTAAAAGTCGCTATAAAGTATGGTAGAGACAAATTGACACTCAATCAAATGCTTGACTCCTTGAGACTTAGAGAACTTGAGATAAAGTCTGAAAGGAAAGATAATGAAGTCTTGTTTGTTAGAGATAAGCAAAAGCATAACAAAAATAGAAATTCCTATAACAAGAATATTGGAAACCAAGCTTCAGGAAACCAAACCTACAGAAATAATAATCAAAATCAACACCACAATTCTAACCAAAACAGAGGCCAGAAGAGTTTCTGGAGAGACAATAGGAACTCGAATAAATCCTATCAACCTGCAAGAACTAATGAATCAACACAAATAAGTTGTAACTACTGTCACAAAGAAGGGCATTAA

Coding sequence (CDS)

ATGTCTGCGAAAGTGGAATTGGAGAAATTCGACTGGAAGGGTGACTTCGGGCTATGGAGGAGAAAGATGAAAGCTCTTCTAGTCCAACAGAAGGTTGCTAACTTCTTGAACCAGGAACATTTCTTCACCTATAAAATGGATTCGAACAAAAACCTTGAGGAAAATCTTGATGATTTTAATACAACTTGTACTGAATTAGCTAATGCCGGTGAAGAAATAAAATCTCAAGATCATGTTGTAATCCTCTTAAAGTCTTTGCTCGAATCTTATAAAGAAGTAAAAGTCGCTATAAAGTATGGTAGAGACAAATTGACACTCAATCAAATGCTTGACTCCTTGAGACTTAGAGAACTTGAGATAAAGTCTGAAAGGAAAGATAATGAAGTCTTGTTTGTTAGAGATAAGCAAAAGCATAACAAAAATAGAAATTCCTATAACAAGAATATTGGAAACCAAGCTTCAGGAAACCAAACCTACAGAAATAATAATCAAAATCAACACCACAATTCTAACCAAAACAGAGGCCAGAAGAGTTTCTGGAGAGACAATAGGAACTCGAATAAATCCTATCAACCTGCAAGAACTAATGAATCAACACAAATAAGTTGTAACTACTGTCACAAAGAAGGGCATTAA

Protein sequence

MSAKVELEKFDWKGDFGLWRRKMKALLVQQKVANFLNQEHFFTYKMDSNKNLEENLDDFNTTCTELANAGEEIKSQDHVVILLKSLLESYKEVKVAIKYGRDKLTLNQMLDSLRLRELEIKSERKDNEVLFVRDKQKHNKNRNSYNKNIGNQASGNQTYRNNNQNQHHNSNQNRGQKSFWRDNRNSNKSYQPARTNESTQISCNYCHKEGH
Homology
BLAST of Lag0028601 vs. NCBI nr
Match: KAA0067607.1 (retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa] >TYJ97155.1 retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa])

HSP 1 Score: 118.2 bits (295), Expect = 8.3e-23
Identity = 77/213 (36.15%), Postives = 108/213 (50.70%), Query Frame = 0

Query: 2   SAKVELEKFDWKGDFGLWRRKMKALLVQQKVANFL------------------------- 61
           S + E+ KF+  GDF +WR+K++ +LVQ KVA  L                         
Sbjct: 3   STRFEMSKFNGNGDFAIWRKKIRVILVQHKVAKILDEEKLPENITESEKRDMDEMTYSTI 62

Query: 62  -----------------------------------NQEHFFTYKMDSNKNLEENLDDFNT 121
                                              N+E FF YKMD +K+LEENLD+F  
Sbjct: 63  LLYLSDEVLRLVDEATTTGELWKKLESLYLTKFLPNKEKFFRYKMDQSKSLEENLDEFQK 122

Query: 122 TCTELANAGEEIKSQDHVVILLKSLLESYKEVKVAIKYGRDKLTLNQMLDSLRLRELEIK 151
              +L+N GE++  ++  VILL SL E+Y+EVK AIKYGRD LT++ +LD+L+ R LEIK
Sbjct: 123 IIVDLSNIGEKMSDENQAVILLNSLPETYREVKAAIKYGRDSLTMSIVLDALKTRNLEIK 182

BLAST of Lag0028601 vs. NCBI nr
Match: TYK25306.1 (putative gag-pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 114.8 bits (286), Expect = 9.2e-22
Identity = 85/274 (31.02%), Postives = 116/274 (42.34%), Query Frame = 0

Query: 2   SAKVELEKFDWKGDFGLWRRKMKALLVQQKVANFLNQEH--------------------- 61
           S + E+ KF+  GDF LWR+K++A+LVQ KVA  L++E                      
Sbjct: 3   STRFEVSKFNGHGDFALWRKKIRAILVQHKVAKILDEERLPDNITESEKRDMDEMAYSTI 62

Query: 62  -------------------------------------------FFTYKMDSNKNLEENLD 121
                                                      FF YKMD +K+LEENLD
Sbjct: 63  LLYLSDEVLRLVDEATTTGELWKKLESLYLTKSLPNKIYIKEKFFGYKMDQSKSLEENLD 122

Query: 122 DFNTTCTELANAGEEIKSQDHVVILLKSLLESYKEVKVAIKYGRDKLTLNQMLDSLRLRE 181
           +F     +L N GE++  ++  VILL SL E+Y+EVK AIKYGRD LT++ +LD+L+ R 
Sbjct: 123 EFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVKAAIKYGRDSLTMSIVLDALKTRN 182

Query: 182 LEIKSERKDNEVLFVRDKQKHNKNRNSYNKNIGNQASGNQTYRNNNQNQHHNSNQNRGQK 212
           LEIK ERKD E+L  R                                        R +K
Sbjct: 183 LEIKKERKDGELLMAR---------------------------------------GRSEK 232

BLAST of Lag0028601 vs. NCBI nr
Match: KAA0050719.1 (putative gag-pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 114.8 bits (286), Expect = 9.2e-22
Identity = 85/274 (31.02%), Postives = 116/274 (42.34%), Query Frame = 0

Query: 2   SAKVELEKFDWKGDFGLWRRKMKALLVQQKVANFLNQEH--------------------- 61
           S + E+ KF+  GDF LWR+K++A+LVQ KVA  L++E                      
Sbjct: 3   STRFEVSKFNGHGDFSLWRKKIRAILVQHKVAKILDEERLPDNITESEKRDMDEMAYSTI 62

Query: 62  -------------------------------------------FFTYKMDSNKNLEENLD 121
                                                      FF YKMD +K+LEENLD
Sbjct: 63  LLYLSDEVLRLVDEATTTGELWKKLESLYLTKSLLNKIYIKEKFFGYKMDQSKSLEENLD 122

Query: 122 DFNTTCTELANAGEEIKSQDHVVILLKSLLESYKEVKVAIKYGRDKLTLNQMLDSLRLRE 181
           +F     +L N GE++  ++  VILL SL E+Y+EVK AIKYGRD LT++ +LD+L+ R 
Sbjct: 123 EFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVKAAIKYGRDSLTMSIVLDALKTRN 182

Query: 182 LEIKSERKDNEVLFVRDKQKHNKNRNSYNKNIGNQASGNQTYRNNNQNQHHNSNQNRGQK 212
           LEIK ERKD E+L  R                                        R +K
Sbjct: 183 LEIKKERKDGELLMAR---------------------------------------GRSEK 232

BLAST of Lag0028601 vs. NCBI nr
Match: TXG70425.1 (hypothetical protein EZV62_005360 [Acer yangbiense])

HSP 1 Score: 110.5 bits (275), Expect = 1.7e-20
Identity = 68/176 (38.64%), Postives = 103/176 (58.52%), Query Frame = 0

Query: 1   MSAKVELEKFDWKGDFGLWRRKMKALLVQQKVANFLNQE--------------------- 60
           +S K E+EKF+ KGDF +WR+KMKA+LVQQK A  L +E                     
Sbjct: 2   VSTKFEIEKFNGKGDFRMWRKKMKAILVQQKCAKSLEEEKDLPVSLSAEEKQDLLETAYN 61

Query: 61  --------HFFTYKMDSNKNLEENLDDFNTTCTELAN--AGEEIKSQDHVVILLKSLLES 120
                   + F +KMD +K+LE+NLDDF     ELAN  + E +  ++  VI+L  L +S
Sbjct: 62  TIILNLADNLFGFKMDQSKSLEDNLDDFTKLNIELANCDSKEALSDENQAVIVLNFLSDS 121

Query: 121 YKEVKVAIKYGRDKLTLNQMLDSLRLRELEIKSERKDNEVLFVRDKQKHNKNRNSY 146
           YK++K AIKYGRD L++  +L +LR RE+E++SE++ +    +  + ++ K  N+Y
Sbjct: 122 YKDLKAAIKYGRDSLSIEDVLGALRSREMEMRSEKRASIGEGLNVRGRYEKKGNNY 177

BLAST of Lag0028601 vs. NCBI nr
Match: KAA0047995.1 (retrotransposon protein, putative, Ty1-copia sub-class [Cucumis melo var. makuwa])

HSP 1 Score: 110.2 bits (274), Expect = 2.3e-20
Identity = 84/274 (30.66%), Postives = 114/274 (41.61%), Query Frame = 0

Query: 2   SAKVELEKFDWKGDFGLWRRKMKALLVQQKVANFLNQEH--------------------- 61
           S + E+ KF+  GDF LWR+K++A+LVQ KVA  L++E                      
Sbjct: 3   STRFEVSKFNGHGDFALWRKKIRAILVQHKVAKILDEERLPDNITESEKRDMDEMAYWTI 62

Query: 62  -------------------------------------------FFTYKMDSNKNLEENLD 121
                                                      FF YKMD +K LEENLD
Sbjct: 63  LLYLSDEVLRLVDEATTTGELWKKLESLYLTKSLPNKIYIKEKFFGYKMDQSKILEENLD 122

Query: 122 DFNTTCTELANAGEEIKSQDHVVILLKSLLESYKEVKVAIKYGRDKLTLNQMLDSLRLRE 181
           +F     +L N GE++  ++  VILL SL E+Y+EVK AIKYG D LT++ +LD+L+ R 
Sbjct: 123 EFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVKAAIKYGWDSLTMSIVLDALKTRN 182

Query: 182 LEIKSERKDNEVLFVRDKQKHNKNRNSYNKNIGNQASGNQTYRNNNQNQHHNSNQNRGQK 212
           LEIK ERKD E+L  R                                        R +K
Sbjct: 183 LEIKKERKDGELLMAR---------------------------------------GRSEK 232

BLAST of Lag0028601 vs. ExPASy TrEMBL
Match: A0A5A7VKC2 (Retrotransposon protein, putative, Ty1-copia subclass OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold174G00310 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 4.0e-23
Identity = 77/213 (36.15%), Postives = 108/213 (50.70%), Query Frame = 0

Query: 2   SAKVELEKFDWKGDFGLWRRKMKALLVQQKVANFL------------------------- 61
           S + E+ KF+  GDF +WR+K++ +LVQ KVA  L                         
Sbjct: 3   STRFEMSKFNGNGDFAIWRKKIRVILVQHKVAKILDEEKLPENITESEKRDMDEMTYSTI 62

Query: 62  -----------------------------------NQEHFFTYKMDSNKNLEENLDDFNT 121
                                              N+E FF YKMD +K+LEENLD+F  
Sbjct: 63  LLYLSDEVLRLVDEATTTGELWKKLESLYLTKFLPNKEKFFRYKMDQSKSLEENLDEFQK 122

Query: 122 TCTELANAGEEIKSQDHVVILLKSLLESYKEVKVAIKYGRDKLTLNQMLDSLRLRELEIK 151
              +L+N GE++  ++  VILL SL E+Y+EVK AIKYGRD LT++ +LD+L+ R LEIK
Sbjct: 123 IIVDLSNIGEKMSDENQAVILLNSLPETYREVKAAIKYGRDSLTMSIVLDALKTRNLEIK 182

BLAST of Lag0028601 vs. ExPASy TrEMBL
Match: A0A5D3DNU1 (Putative gag-pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004440 PE=4 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 4.5e-22
Identity = 85/274 (31.02%), Postives = 116/274 (42.34%), Query Frame = 0

Query: 2   SAKVELEKFDWKGDFGLWRRKMKALLVQQKVANFLNQEH--------------------- 61
           S + E+ KF+  GDF LWR+K++A+LVQ KVA  L++E                      
Sbjct: 3   STRFEVSKFNGHGDFALWRKKIRAILVQHKVAKILDEERLPDNITESEKRDMDEMAYSTI 62

Query: 62  -------------------------------------------FFTYKMDSNKNLEENLD 121
                                                      FF YKMD +K+LEENLD
Sbjct: 63  LLYLSDEVLRLVDEATTTGELWKKLESLYLTKSLPNKIYIKEKFFGYKMDQSKSLEENLD 122

Query: 122 DFNTTCTELANAGEEIKSQDHVVILLKSLLESYKEVKVAIKYGRDKLTLNQMLDSLRLRE 181
           +F     +L N GE++  ++  VILL SL E+Y+EVK AIKYGRD LT++ +LD+L+ R 
Sbjct: 123 EFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVKAAIKYGRDSLTMSIVLDALKTRN 182

Query: 182 LEIKSERKDNEVLFVRDKQKHNKNRNSYNKNIGNQASGNQTYRNNNQNQHHNSNQNRGQK 212
           LEIK ERKD E+L  R                                        R +K
Sbjct: 183 LEIKKERKDGELLMAR---------------------------------------GRSEK 232

BLAST of Lag0028601 vs. ExPASy TrEMBL
Match: A0A5A7UB25 (Putative gag-pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold560G00190 PE=4 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 4.5e-22
Identity = 85/274 (31.02%), Postives = 116/274 (42.34%), Query Frame = 0

Query: 2   SAKVELEKFDWKGDFGLWRRKMKALLVQQKVANFLNQEH--------------------- 61
           S + E+ KF+  GDF LWR+K++A+LVQ KVA  L++E                      
Sbjct: 3   STRFEVSKFNGHGDFSLWRKKIRAILVQHKVAKILDEERLPDNITESEKRDMDEMAYSTI 62

Query: 62  -------------------------------------------FFTYKMDSNKNLEENLD 121
                                                      FF YKMD +K+LEENLD
Sbjct: 63  LLYLSDEVLRLVDEATTTGELWKKLESLYLTKSLLNKIYIKEKFFGYKMDQSKSLEENLD 122

Query: 122 DFNTTCTELANAGEEIKSQDHVVILLKSLLESYKEVKVAIKYGRDKLTLNQMLDSLRLRE 181
           +F     +L N GE++  ++  VILL SL E+Y+EVK AIKYGRD LT++ +LD+L+ R 
Sbjct: 123 EFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVKAAIKYGRDSLTMSIVLDALKTRN 182

Query: 182 LEIKSERKDNEVLFVRDKQKHNKNRNSYNKNIGNQASGNQTYRNNNQNQHHNSNQNRGQK 212
           LEIK ERKD E+L  R                                        R +K
Sbjct: 183 LEIKKERKDGELLMAR---------------------------------------GRSEK 232

BLAST of Lag0028601 vs. ExPASy TrEMBL
Match: A0A5A7U2U7 (Retrotransposon protein, putative, Ty1-copia sub-class OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00590 PE=4 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 1.1e-20
Identity = 84/274 (30.66%), Postives = 114/274 (41.61%), Query Frame = 0

Query: 2   SAKVELEKFDWKGDFGLWRRKMKALLVQQKVANFLNQEH--------------------- 61
           S + E+ KF+  GDF LWR+K++A+LVQ KVA  L++E                      
Sbjct: 3   STRFEVSKFNGHGDFALWRKKIRAILVQHKVAKILDEERLPDNITESEKRDMDEMAYWTI 62

Query: 62  -------------------------------------------FFTYKMDSNKNLEENLD 121
                                                      FF YKMD +K LEENLD
Sbjct: 63  LLYLSDEVLRLVDEATTTGELWKKLESLYLTKSLPNKIYIKEKFFGYKMDQSKILEENLD 122

Query: 122 DFNTTCTELANAGEEIKSQDHVVILLKSLLESYKEVKVAIKYGRDKLTLNQMLDSLRLRE 181
           +F     +L N GE++  ++  VILL SL E+Y+EVK AIKYG D LT++ +LD+L+ R 
Sbjct: 123 EFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVKAAIKYGWDSLTMSIVLDALKTRN 182

Query: 182 LEIKSERKDNEVLFVRDKQKHNKNRNSYNKNIGNQASGNQTYRNNNQNQHHNSNQNRGQK 212
           LEIK ERKD E+L  R                                        R +K
Sbjct: 183 LEIKKERKDGELLMAR---------------------------------------GRSEK 232

BLAST of Lag0028601 vs. ExPASy TrEMBL
Match: A0A5D3BRB2 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2454G00070 PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 2.5e-20
Identity = 75/220 (34.09%), Postives = 105/220 (47.73%), Query Frame = 0

Query: 2   SAKVELEKFDWKGDFGLWRRKMKALLVQQKVANFLNQ----------------------- 61
           S + E+ KF+  GDF LWR+K++A+LVQ KVA  L++                       
Sbjct: 3   STRFEVSKFNVNGDFALWRKKIRAILVQHKVAKILDEGRLPANITENEKRDMDEMAYSTI 62

Query: 62  ------------------------------------------EHFFTYKMDSNKNLEENL 121
                                                     E FF YKMD +K+LEENL
Sbjct: 63  LMYLSVEVLRLVDETTTTTAELWKKLESLYLTKSLPNKIYIKEKFFGYKMDQSKSLEENL 122

Query: 122 DDFNTTCTELANAGEEIKSQDHVVILLKSLLESYKEVKVAIKYGRDKLTLNQMLDSLRLR 154
           ++F     +L N GE++  ++  +ILL SL E+Y+EVK AIKYGRD LT++ + D+L+ R
Sbjct: 123 NEFQKIVVDLNNIGEKMSDENQAIILLNSLPETYREVKAAIKYGRDSLTMSIVFDALKTR 182

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0067607.18.3e-2336.15retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa]... [more]
TYK25306.19.2e-2231.02putative gag-pol polyprotein [Cucumis melo var. makuwa][more]
KAA0050719.19.2e-2231.02putative gag-pol polyprotein [Cucumis melo var. makuwa][more]
TXG70425.11.7e-2038.64hypothetical protein EZV62_005360 [Acer yangbiense][more]
KAA0047995.12.3e-2030.66retrotransposon protein, putative, Ty1-copia sub-class [Cucumis melo var. makuwa... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7VKC24.0e-2336.15Retrotransposon protein, putative, Ty1-copia subclass OS=Cucumis melo var. makuw... [more]
A0A5D3DNU14.5e-2231.02Putative gag-pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A5A7UB254.5e-2231.02Putative gag-pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A5A7U2U71.1e-2030.66Retrotransposon protein, putative, Ty1-copia sub-class OS=Cucumis melo var. maku... [more]
A0A5D3BRB22.5e-2034.09Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var.... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 49..69
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 35..116
e-value: 1.5E-14
score: 53.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 132..198
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 143..198

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0028601.1Lag0028601.1mRNA