Lag0028589 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0028589
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein CROWDED NUCLEI 3
Locationchr8: 25884408 .. 25884749 (-)
RNA-Seq ExpressionLag0028589
SyntenyLag0028589
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCACGCCTCAGAGGAAGGGGTGGCCTGCTGTGTCCCTCACGCCTCGAAGCGAAGCAAAAGTTGCAGTTTCCAATTCCATTGTTGTGGGGAAAGGAAAGGCTGTGGCTTTTACAGACGGCCCTCCGCCGCCGTTGGGCTCTCTGAACGACGGATTGCATAAAACTGCGGCGGCTGTGGATACTGGGGACATGGACGATTGGAGGAAGTTTAAGAAGGCGGGGTTGTTGGATGCGGCCGTAATGGAGCGCAAAGATCGGGAGGCACTTCTCGAGAAGGCTTCAAGGCTTCAAAGCGAGGTTAGCAATTATCTTCGAGTCTTACTCGTTTTCTATCATTAG

mRNA sequence

ATGTTCACGCCTCAGAGGAAGGGGTGGCCTGCTGTGTCCCTCACGCCTCGAAGCGAAGCAAAAGTTGCAGTTTCCAATTCCATTGTTGTGGGGAAAGGAAAGGCTGTGGCTTTTACAGACGGCCCTCCGCCGCCGTTGGGCTCTCTGAACGACGGATTGCATAAAACTGCGGCGGCTGTGGATACTGGGGACATGGACGATTGGAGGAAGTTTAAGAAGGCGGGGTTGTTGGATGCGGCCGTAATGGAGCGCAAAGATCGGGAGGCACTTCTCGAGAAGGCTTCAAGGCTTCAAAGCGAGGTTAGCAATTATCTTCGAGTCTTACTCGTTTTCTATCATTAG

Coding sequence (CDS)

ATGTTCACGCCTCAGAGGAAGGGGTGGCCTGCTGTGTCCCTCACGCCTCGAAGCGAAGCAAAAGTTGCAGTTTCCAATTCCATTGTTGTGGGGAAAGGAAAGGCTGTGGCTTTTACAGACGGCCCTCCGCCGCCGTTGGGCTCTCTGAACGACGGATTGCATAAAACTGCGGCGGCTGTGGATACTGGGGACATGGACGATTGGAGGAAGTTTAAGAAGGCGGGGTTGTTGGATGCGGCCGTAATGGAGCGCAAAGATCGGGAGGCACTTCTCGAGAAGGCTTCAAGGCTTCAAAGCGAGGTTAGCAATTATCTTCGAGTCTTACTCGTTTTCTATCATTAG

Protein sequence

MFTPQRKGWPAVSLTPRSEAKVAVSNSIVVGKGKAVAFTDGPPPPLGSLNDGLHKTAAAVDTGDMDDWRKFKKAGLLDAAVMERKDREALLEKASRLQSEVSNYLRVLLVFYH
Homology
BLAST of Lag0028589 vs. NCBI nr
Match: XP_004147138.1 (protein CROWDED NUCLEI 3 [Cucumis sativus] >KAE8648619.1 hypothetical protein Csa_008601 [Cucumis sativus])

HSP 1 Score: 176.4 bits (446), Expect = 1.4e-40
Identity = 85/104 (81.73%), Postives = 94/104 (90.38%), Query Frame = 0

Query: 1   MFTPQRKGWPAVSLTPRSEAKVAVSNSIVVGKGKAVAFTDGPPPPLGSLNDGLHKTAAAV 60
           MFTPQR GWPA SLTPR+E K+A++NSI++GKGK V FTD PPPPLGSLND L+KTA AV
Sbjct: 1   MFTPQRTGWPAASLTPRTEPKLALTNSIILGKGKDVTFTDDPPPPLGSLNDELYKTATAV 60

Query: 61  DTGDMDDWRKFKKAGLLDAAVMERKDREALLEKASRLQSEVSNY 105
           DTGDMDDWRKFKKAGLLDAA MERKDREALLEKASRLQSE+ +Y
Sbjct: 61  DTGDMDDWRKFKKAGLLDAAAMERKDREALLEKASRLQSELLDY 104

BLAST of Lag0028589 vs. NCBI nr
Match: XP_008467201.1 (PREDICTED: protein CROWDED NUCLEI 3 [Cucumis melo])

HSP 1 Score: 176.4 bits (446), Expect = 1.4e-40
Identity = 86/104 (82.69%), Postives = 94/104 (90.38%), Query Frame = 0

Query: 1   MFTPQRKGWPAVSLTPRSEAKVAVSNSIVVGKGKAVAFTDGPPPPLGSLNDGLHKTAAAV 60
           MFTPQR GWPA SLTPR+E K+A+SNSI++GKGK V FTD PPPPLGSLND L+KTA AV
Sbjct: 1   MFTPQRTGWPAASLTPRTEPKLALSNSILLGKGKDVIFTDDPPPPLGSLNDELYKTATAV 60

Query: 61  DTGDMDDWRKFKKAGLLDAAVMERKDREALLEKASRLQSEVSNY 105
           DTGDMDDWRKFKKAGLLDAA MERKDREALLEKASRLQSE+ +Y
Sbjct: 61  DTGDMDDWRKFKKAGLLDAAAMERKDREALLEKASRLQSELFDY 104

BLAST of Lag0028589 vs. NCBI nr
Match: TYK00277.1 (protein CROWDED NUCLEI 3 [Cucumis melo var. makuwa])

HSP 1 Score: 174.9 bits (442), Expect = 4.0e-40
Identity = 87/109 (79.82%), Postives = 96/109 (88.07%), Query Frame = 0

Query: 1   MFTPQRKGWPAVSLTPRSEAKVAVSNSIVVGKGKAVAFTDGPPPPLGSLNDGLHKTAAAV 60
           MFTPQR GWPA SLTPR+E K+A+SNSI++GKGK V FTD PPPPLGSLND L+KTA AV
Sbjct: 1   MFTPQRTGWPAASLTPRTEPKLALSNSILLGKGKDVIFTDDPPPPLGSLNDELYKTATAV 60

Query: 61  DTGDMDDWRKFKKAGLLDAAVMERKDREALLEKASRLQSEVSNYLRVLL 110
           DTGDMDDWRKFKKAGLLDAA MERKDREALLEKASRLQSEV  ++  L+
Sbjct: 61  DTGDMDDWRKFKKAGLLDAAAMERKDREALLEKASRLQSEVXFFVFSLI 109

BLAST of Lag0028589 vs. NCBI nr
Match: XP_022158849.1 (protein CROWDED NUCLEI 1-like isoform X2 [Momordica charantia])

HSP 1 Score: 174.5 bits (441), Expect = 5.3e-40
Identity = 90/109 (82.57%), Postives = 94/109 (86.24%), Query Frame = 0

Query: 1   MFTPQRKGWPAVSLTPRSEAKVAVSNSIVVGKGKA-----VAFTDGPPPPLGSLNDGLHK 60
           MFTPQRKGWPAVSLTPRSEAK AVSNSI+VGKGK      VA TD PPP LGSL DGLH 
Sbjct: 1   MFTPQRKGWPAVSLTPRSEAKFAVSNSILVGKGKGKGYKDVALTDAPPPSLGSLTDGLHT 60

Query: 61  TAAAVDTGDMDDWRKFKKAGLLDAAVMERKDREALLEKASRLQSEVSNY 105
           TAAAVDT DMDDWRKFKKAGLLDAA MERKDREALLEKASRLQ+E+ +Y
Sbjct: 61  TAAAVDTEDMDDWRKFKKAGLLDAAAMERKDREALLEKASRLQTELFDY 109

BLAST of Lag0028589 vs. NCBI nr
Match: XP_022158848.1 (protein CROWDED NUCLEI 1-like isoform X1 [Momordica charantia])

HSP 1 Score: 174.5 bits (441), Expect = 5.3e-40
Identity = 90/109 (82.57%), Postives = 94/109 (86.24%), Query Frame = 0

Query: 1   MFTPQRKGWPAVSLTPRSEAKVAVSNSIVVGKGKA-----VAFTDGPPPPLGSLNDGLHK 60
           MFTPQRKGWPAVSLTPRSEAK AVSNSI+VGKGK      VA TD PPP LGSL DGLH 
Sbjct: 1   MFTPQRKGWPAVSLTPRSEAKFAVSNSILVGKGKGKGYKDVALTDAPPPSLGSLTDGLHT 60

Query: 61  TAAAVDTGDMDDWRKFKKAGLLDAAVMERKDREALLEKASRLQSEVSNY 105
           TAAAVDT DMDDWRKFKKAGLLDAA MERKDREALLEKASRLQ+E+ +Y
Sbjct: 61  TAAAVDTEDMDDWRKFKKAGLLDAAAMERKDREALLEKASRLQTELFDY 109

BLAST of Lag0028589 vs. ExPASy Swiss-Prot
Match: Q9SAF6 (Protein CROWDED NUCLEI 2 OS=Arabidopsis thaliana OX=3702 GN=CRWN2 PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 9.7e-13
Identity = 48/100 (48.00%), Postives = 63/100 (63.00%), Query Frame = 0

Query: 14  LTPRSEA-KV-AVSNSIVVG-KGKAVAFTDG------PPPPLGSLNDGLHKTAAAVDTGD 73
           +TPRSE  K+  V+N      KGKAVAF+D       PPPP+G+L  G   +    D  D
Sbjct: 1   MTPRSETHKIGGVTNPRNADRKGKAVAFSDDLVIPTLPPPPIGTLT-GQGVSRGHTDDMD 60

Query: 74  MDDWRKFKKAGLLDAAVMERKDREALLEKASRLQSEVSNY 105
           M DWR+F++ GLL+ A ME+KD+EALLEK S L+ E+  Y
Sbjct: 61  MGDWRRFREVGLLNEASMEKKDQEALLEKISTLEKELYGY 99

BLAST of Lag0028589 vs. ExPASy Swiss-Prot
Match: Q9CA42 (Protein CROWDED NUCLEI 3 OS=Arabidopsis thaliana OX=3702 GN=CRWN3 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 3.5e-10
Identity = 42/107 (39.25%), Postives = 54/107 (50.47%), Query Frame = 0

Query: 1   MFTPQRKGWPAVSLTPRSEAKVAVSNSIVVGKGKAVAFTD---GPPPPLGSLNDGLHKTA 60
           MFTPQR  WP                     KGKA+AF+D    PPP    L +      
Sbjct: 1   MFTPQRNRWPETD-----------------RKGKAIAFSDEIITPPPQRVLLRED----- 60

Query: 61  AAVDTGDMDDWRKFKKAGLLDAAVMERKDREALLEKASRLQSEVSNY 105
                   DDW+KFK+ GLLD A +ERKDR+AL+EK  +L+ E+ +Y
Sbjct: 61  --------DDWQKFKEVGLLDEASLERKDRDALIEKILKLEKELFDY 77

BLAST of Lag0028589 vs. ExPASy Swiss-Prot
Match: I0J0E7 (Nuclear matrix constituent protein 1 OS=Allium cepa OX=4679 GN=NMCP1 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 6.1e-07
Identity = 39/110 (35.45%), Postives = 53/110 (48.18%), Query Frame = 0

Query: 1   MFTPQRKGWPAVSLT----PRSEAKVAVSNSIVVGKGKAVAFTDGPPPPLGSLNDGLHKT 60
           M TPQR  W   S      PRS+ K         G  K +     P PPLG LN G    
Sbjct: 1   MLTPQRSAWSLKSKVSSEKPRSKGK---------GITKNLDSAATPFPPLGLLNGG---- 60

Query: 61  AAAVDTG--DMDDWRKFKKAGLLDAAVMERKDREALLEKASRLQSEVSNY 105
              +D G  DM+ W++FK  GLLD ++  +KDRE+L  +   L+ ++  Y
Sbjct: 61  --DLDRGGEDMEAWKRFKDEGLLDESICYKKDRESLASRIIELEKDLHEY 95

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004147138.11.4e-4081.73protein CROWDED NUCLEI 3 [Cucumis sativus] >KAE8648619.1 hypothetical protein Cs... [more]
XP_008467201.11.4e-4082.69PREDICTED: protein CROWDED NUCLEI 3 [Cucumis melo][more]
TYK00277.14.0e-4079.82protein CROWDED NUCLEI 3 [Cucumis melo var. makuwa][more]
XP_022158849.15.3e-4082.57protein CROWDED NUCLEI 1-like isoform X2 [Momordica charantia][more]
XP_022158848.15.3e-4082.57protein CROWDED NUCLEI 1-like isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9SAF69.7e-1348.00Protein CROWDED NUCLEI 2 OS=Arabidopsis thaliana OX=3702 GN=CRWN2 PE=1 SV=1[more]
Q9CA423.5e-1039.25Protein CROWDED NUCLEI 3 OS=Arabidopsis thaliana OX=3702 GN=CRWN3 PE=1 SV=1[more]
I0J0E76.1e-0735.45Nuclear matrix constituent protein 1 OS=Allium cepa OX=4679 GN=NMCP1 PE=2 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 87..107
NoneNo IPR availablePANTHERPTHR31908:SF9PROTEIN CROWDED NUCLEI 3coord: 1..104
IPR040418Protein crowded nucleiPANTHERPTHR31908PROTEIN CROWDED NUCLEI 4coord: 1..104

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0028589.1Lag0028589.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006997 nucleus organization
cellular_component GO:0005652 nuclear lamina
cellular_component GO:0005634 nucleus