Lag0028170 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0028170
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Ribonuclease H
Location	chr8: 14846417 .. 14846980 (+)
RNA-Seq Expression	Lag0028170
Synteny	Lag0028170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGGGGCTGTTGAAGCTGCAAACAAAAACATAAAAAGAATTGTTGAAAAGATGACTATAACGTACAAAGATTGGCATGAAATGCTCCCCTTCGCTCTACACGGCTATCGCACGTCAGTTCGTACATCAACTGGGGCAACGCCTTTTTCCTTAGTATATGGTATGGAGGTTGTGCTCCCAATTGAGGTGAAAATTCCATCGCTAAGAGTTCTTATGGAAGCTATGGAAGAGGCTGAATGGGTCCAGAATCGCTATGAACAGCTCAAATTCGTTGAAGAGAAGAGATTAACAGCATTTAGTAGAGGTCAACTCTATCAAAGAAGGATGATGAGAGCATATGATAAGAAAGTTCGCCCAAGAAGATTTAGAGAGGGAGATCTGGTGTTGAAAAGGATCCTTCCATTTCAAAAGGATCATCGAGGAAAATGGACTCCAAATTATGAAGGACCATTTGTGGTGAAAAAGGCTTTCTCTGGTGGGGCATTAATCTTAACCAATATCGATGGATGTGAATTATCAAGCCCAGTCAATTCAGACTATGTTCGGAAGTATTATACATAG

mRNA sequence

ATGAATGGGGCTGTTGAAGCTGCAAACAAAAACATAAAAAGAATTGTTGAAAAGATGACTATAACGTACAAAGATTGGCATGAAATGCTCCCCTTCGCTCTACACGGCTATCGCACGTCAGTTCGTACATCAACTGGGGCAACGCCTTTTTCCTTAGTATATGGTATGGAGGTTGTGCTCCCAATTGAGGTGAAAATTCCATCGCTAAGAGTTCTTATGGAAGCTATGGAAGAGGCTGAATGGGTCCAGAATCGCTATGAACAGCTCAAATTCGTTGAAGAGAAGAGATTAACAGCATTTAGTAGAGGTCAACTCTATCAAAGAAGGATGATGAGAGCATATGATAAGAAAGTTCGCCCAAGAAGATTTAGAGAGGGAGATCTGGTGTTGAAAAGGATCCTTCCATTTCAAAAGGATCATCGAGGAAAATGGACTCCAAATTATGAAGGACCATTTGTGGTGAAAAAGGCTTTCTCTGGTGGGGCATTAATCTTAACCAATATCGATGGATGTGAATTATCAAGCCCAGTCAATTCAGACTATGTTCGGAAGTATTATACATAG

Coding sequence (CDS)

ATGAATGGGGCTGTTGAAGCTGCAAACAAAAACATAAAAAGAATTGTTGAAAAGATGACTATAACGTACAAAGATTGGCATGAAATGCTCCCCTTCGCTCTACACGGCTATCGCACGTCAGTTCGTACATCAACTGGGGCAACGCCTTTTTCCTTAGTATATGGTATGGAGGTTGTGCTCCCAATTGAGGTGAAAATTCCATCGCTAAGAGTTCTTATGGAAGCTATGGAAGAGGCTGAATGGGTCCAGAATCGCTATGAACAGCTCAAATTCGTTGAAGAGAAGAGATTAACAGCATTTAGTAGAGGTCAACTCTATCAAAGAAGGATGATGAGAGCATATGATAAGAAAGTTCGCCCAAGAAGATTTAGAGAGGGAGATCTGGTGTTGAAAAGGATCCTTCCATTTCAAAAGGATCATCGAGGAAAATGGACTCCAAATTATGAAGGACCATTTGTGGTGAAAAAGGCTTTCTCTGGTGGGGCATTAATCTTAACCAATATCGATGGATGTGAATTATCAAGCCCAGTCAATTCAGACTATGTTCGGAAGTATTATACATAG

Protein sequence

MNGAVEAANKNIKRIVEKMTITYKDWHEMLPFALHGYRTSVRTSTGATPFSLVYGMEVVLPIEVKIPSLRVLMEAMEEAEWVQNRYEQLKFVEEKRLTAFSRGQLYQRRMMRAYDKKVRPRRFREGDLVLKRILPFQKDHRGKWTPNYEGPFVVKKAFSGGALILTNIDGCELSSPVNSDYVRKYYT

Homology

[Close]

BLAST of Lag0028170 vs. NCBI nr
Match: KAA0037621.1 (uncharacterized protein E6C27_scaffold277G002820 [Cucumis melo var. makuwa])

HSP 1 Score: 331.3 bits (848), Expect = 5.6e-87
Identity = 161/187 (86.10%), Postives = 175/187 (93.58%), Query Frame = 0


Query: 1    MNGAVEAANKNIKRIVEKMTITYKDWHEMLPFALHGYRTSVRTSTGATPFSLVYGMEVVL 60
            MNGAVEAANKNIKRI+EKMTITYKDWHEMLPFALHGYRTSVRTSTGATPFSLVYGME VL
Sbjct: 1751 MNGAVEAANKNIKRIIEKMTITYKDWHEMLPFALHGYRTSVRTSTGATPFSLVYGMEAVL 1810

Query: 61   PIEVKIPSLRVLMEA-MEEAEWVQNRYEQLKFVEEKRLTAFSRGQLYQRRMMRAYDKKVR 120
            P+EV+IPSLRVLMEA ++EAEW++ RYEQL FVEEKRL A +  QLYQRR+MRAY+KKV 
Sbjct: 1811 PLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFVEEKRLAALNHVQLYQRRLMRAYNKKVH 1870

Query: 121  PRRFREGDLVLKRILPFQKDHRGKWTPNYEGPFVVKKAFSGGALILTNIDGCELSSPVNS 180
            PR FREGDLVLKRILPFQKDHRGKWTPNYEGPFVVKKAFSGGAL+LTN+DG EL +PVNS
Sbjct: 1871 PRSFREGDLVLKRILPFQKDHRGKWTPNYEGPFVVKKAFSGGALLLTNMDGIELKNPVNS 1930

Query: 181  DYVRKYY 187
            DYVR+YY
Sbjct: 1931 DYVRRYY 1937

[Close]

BLAST of Lag0028170 vs. NCBI nr
Match: XP_022150030.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111018303 [Momordica charantia])

HSP 1 Score: 330.9 bits (847), Expect = 7.3e-87
Identity = 161/187 (86.10%), Postives = 174/187 (93.05%), Query Frame = 0


Query: 1    MNGAVEAANKNIKRIVEKMTITYKDWHEMLPFALHGYRTSVRTSTGATPFSLVYGMEVVL 60
            MNGAVEAANKNIKRIVEKMT+TY+DWHEMLPFALHGYRTSVRTSTGATPFSLVYGME VL
Sbjct: 1481 MNGAVEAANKNIKRIVEKMTVTYRDWHEMLPFALHGYRTSVRTSTGATPFSLVYGMEAVL 1540

Query: 61   PIEVKIPSLRVLMEA-MEEAEWVQNRYEQLKFVEEKRLTAFSRGQLYQRRMMRAYDKKVR 120
            PIEV+IPSLRV+MEA ++EAEWVQ RYEQL FVEEKRLTA  RGQLYQRRMM+AYDKKV 
Sbjct: 1541 PIEVEIPSLRVIMEAKLQEAEWVQRRYEQLNFVEEKRLTALYRGQLYQRRMMKAYDKKVH 1600

Query: 121  PRRFREGDLVLKRILPFQKDHRGKWTPNYEGPFVVKKAFSGGALILTNIDGCELSSPVNS 180
             RRFREGDLVLKRILP QKDHRGKWTPNYEGPFV+KKAFSGGAL+L N+DG E  +P+NS
Sbjct: 1601 SRRFREGDLVLKRILPLQKDHRGKWTPNYEGPFVLKKAFSGGALVLANMDGTEFLNPINS 1660

Query: 181  DYVRKYY 187
            D+VRKYY
Sbjct: 1661 DHVRKYY 1667

[Close]

BLAST of Lag0028170 vs. NCBI nr
Match: XP_031738551.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC101203611 [Cucumis sativus])

HSP 1 Score: 330.1 bits (845), Expect = 1.2e-86
Identity = 159/187 (85.03%), Postives = 175/187 (93.58%), Query Frame = 0


Query: 1    MNGAVEAANKNIKRIVEKMTITYKDWHEMLPFALHGYRTSVRTSTGATPFSLVYGMEVVL 60
            MNGAVEAANKNIKRI+EKMT TYKDWHE+LPFALHGYRTSVRTSTGATPFSLVYGME VL
Sbjct: 2448 MNGAVEAANKNIKRIIEKMTTTYKDWHEILPFALHGYRTSVRTSTGATPFSLVYGMEAVL 2507

Query: 61   PIEVKIPSLRVLMEA-MEEAEWVQNRYEQLKFVEEKRLTAFSRGQLYQRRMMRAYDKKVR 120
            P+EV+IPSLRVLMEA ++EAEW++ RYEQL F+EEKRLTA S GQLYQ+R+MRAY+KKV 
Sbjct: 2508 PLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFIEEKRLTALSHGQLYQKRLMRAYNKKVH 2567

Query: 121  PRRFREGDLVLKRILPFQKDHRGKWTPNYEGPFVVKKAFSGGALILTNIDGCELSSPVNS 180
            PR FREGDLVLKRILPF KDHRGKWTPNYEGPFVVKKAFSGGAL+LTN+DG EL +PVNS
Sbjct: 2568 PRSFREGDLVLKRILPFLKDHRGKWTPNYEGPFVVKKAFSGGALLLTNMDGVELKNPVNS 2627

Query: 181  DYVRKYY 187
            DYVR+YY
Sbjct: 2628 DYVRRYY 2634

[Close]

BLAST of Lag0028170 vs. NCBI nr
Match: XP_031741309.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC105435093 [Cucumis sativus])

HSP 1 Score: 330.1 bits (845), Expect = 1.2e-86
Identity = 159/187 (85.03%), Postives = 175/187 (93.58%), Query Frame = 0


Query: 1    MNGAVEAANKNIKRIVEKMTITYKDWHEMLPFALHGYRTSVRTSTGATPFSLVYGMEVVL 60
            MNGAVEAANKNIKRI+EKMT TYKDWHE+LPFALHGYRTSVRTSTGATPFSLVYGME VL
Sbjct: 2101 MNGAVEAANKNIKRIIEKMTTTYKDWHEILPFALHGYRTSVRTSTGATPFSLVYGMEAVL 2160

Query: 61   PIEVKIPSLRVLMEA-MEEAEWVQNRYEQLKFVEEKRLTAFSRGQLYQRRMMRAYDKKVR 120
            P+EV+IPSLRVLMEA ++EAEW++ RYEQL F+EEKRLTA S GQLYQ+R+MRAY+KKV 
Sbjct: 2161 PLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFIEEKRLTALSHGQLYQKRLMRAYNKKVH 2220

Query: 121  PRRFREGDLVLKRILPFQKDHRGKWTPNYEGPFVVKKAFSGGALILTNIDGCELSSPVNS 180
            PR FREGDLVLKRILPF KDHRGKWTPNYEGPFVVKKAFSGGAL+LTN+DG EL +PVNS
Sbjct: 2221 PRSFREGDLVLKRILPFLKDHRGKWTPNYEGPFVVKKAFSGGALLLTNMDGVELKNPVNS 2280

Query: 181  DYVRKYY 187
            DYVR+YY
Sbjct: 2281 DYVRRYY 2287

[Close]

BLAST of Lag0028170 vs. NCBI nr
Match: XP_031737561.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC116402452 [Cucumis sativus])

HSP 1 Score: 330.1 bits (845), Expect = 1.2e-86
Identity = 159/187 (85.03%), Postives = 175/187 (93.58%), Query Frame = 0


Query: 1    MNGAVEAANKNIKRIVEKMTITYKDWHEMLPFALHGYRTSVRTSTGATPFSLVYGMEVVL 60
            MNGAVEAANKNIKRI+EKMT TYKDWHE+LPFALHGYRTSVRTSTGATPFSLVYGME VL
Sbjct: 2103 MNGAVEAANKNIKRIIEKMTTTYKDWHEILPFALHGYRTSVRTSTGATPFSLVYGMEAVL 2162

Query: 61   PIEVKIPSLRVLMEA-MEEAEWVQNRYEQLKFVEEKRLTAFSRGQLYQRRMMRAYDKKVR 120
            P+EV+IPSLRVLMEA ++EAEW++ RYEQL F+EEKRLTA S GQLYQ+R+MRAY+KKV 
Sbjct: 2163 PLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFIEEKRLTALSHGQLYQKRLMRAYNKKVH 2222

Query: 121  PRRFREGDLVLKRILPFQKDHRGKWTPNYEGPFVVKKAFSGGALILTNIDGCELSSPVNS 180
            PR FREGDLVLKRILPF KDHRGKWTPNYEGPFVVKKAFSGGAL+LTN+DG EL +PVNS
Sbjct: 2223 PRSFREGDLVLKRILPFLKDHRGKWTPNYEGPFVVKKAFSGGALLLTNMDGVELKNPVNS 2282

Query: 181  DYVRKYY 187
            DYVR+YY
Sbjct: 2283 DYVRRYY 2289

The following BLAST results are available for this feature:

BLAST of Lag0028170 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0037621.1	5.6e-87	86.10	uncharacterized protein E6C27_scaffold277G002820 [Cucumis melo var. makuwa]	[more]
XP_022150030.1	7.3e-87	86.10	LOW QUALITY PROTEIN: uncharacterized protein LOC111018303 [Momordica charantia]	[more]
XP_031738551.1	1.2e-86	85.03	LOW QUALITY PROTEIN: uncharacterized protein LOC101203611 [Cucumis sativus]	[more]
XP_031741309.1	1.2e-86	85.03	LOW QUALITY PROTEIN: uncharacterized protein LOC105435093 [Cucumis sativus]	[more]
XP_031737561.1	1.2e-86	85.03	LOW QUALITY PROTEIN: uncharacterized protein LOC116402452 [Cucumis sativus]	[more]

BLAST of Lag0028170 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 1..101 e-value: 1.5E-15 score: 59.0
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 2..144
None	No IPR available	PANTHER	PTHR24559:SF342	SUBFAMILY NOT NAMED	coord: 2..144
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 2..63

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0028170.1	Lag0028170.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0006310	DNA recombination
biological_process	GO:0006278	RNA-dependent DNA biosynthetic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0030430	host cell cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003887	DNA-directed DNA polymerase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003964	RNA-directed DNA polymerase activity
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity
molecular_function	GO:0003676	nucleic acid binding