Lag0028148 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0028148
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Membrane protein of ER body-like protein
Location	chr8: 14596888 .. 14598449 (-)
RNA-Seq Expression	Lag0028148
Synteny	Lag0028148

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGATTTTTCTAAACTCATTAGAAGAAGTAAACAAAGACGTTGGGTCTTCAGAATCAGTAGTTGTCAACATTCCTCTTGGGAAAAGAGAAACAACTCTCCGACAGAATAGCAATGGAGCTAGAAAGTGGGAAGTGCAGAAAGCCATAGTAGTTGGTGGTTTATTTGAATCAATCACAAGTTTAGTCATTGTAACCGCTGCAGCAAGTGTTAATATTTCCATTGGTAATAGTCCTCAGTATCTCTTTGTATCTCCTTTTTCTTATAATAAATGTTTTTGATATCCATCCATTTACATTCTTTTGAATTATTTTTTCATAATTGCAGAAAATATTGTAGCTTTGGCAATGACAAACTTGATTAGTGGACTTTTTGTCGTTACACATAATGTAAGTAATTACTTTTTCCTCTCTCGAATGCGATAAAGTGGAGAAAGTGTATATGTGCGTCTCATTCTACTAATTCAAATGTTTGAAGTGGAAACTTTGTTTTAAACTATTTAATTATTAGAAATATGTCAAATACATATTCGAAACGAATTAATTATTGTCATTTGTTAAAAGTAAATTATTAATTTGAATTCTTCATATGCATTATTCACTTTCAGCATTTTGAAATGGAGAAGATAAAGGGAAGAATAAAAAGAGAATTGCGAGAGAAAACACTTTGCTTATAATGGACCTAAAACATAATGGGCCAACAAATACAAAATCAGATAAACAAAAAACTTTAGTGGCTTATCTTAACATTCACAACCTATAGATGGGTGTCATTTTGATGCTATCCTTCTCTACTTTAATTTTAGAAATATAATATATAGAAATCACATGACTTAAAGTGTGTCTGGAAATGCTTTCACATATATTAGCTGAGCTGTTTTGTGGGATTAGAAAAAAATTAAGAGTCTATTTGGAATGAATTTCCATGTGTTTAAAAATGTATTTCTAAGTAAAAAGAAATGTTTTGAATTAAAACACTTGAAAAGTCATTCGAGAGAGATTCTAACAGGAATCAATGCAAACACGTTCTTTCTTAAATTGTTTTTTAGCCATATATATTTTGAATACATGACTAACATGCCATTATAAAACACAAAAAAGGTTAAATTATTATTTTGTTACTAAAGTTGAATTTATGAATAAATGATTTACAGCTATCAGAACTGAAGATCAAGAATAGGCTTGAGAAGGAATATAATGGAGTTGAAGTAGAAGGAATACAGTATAAAGAAGTATTGATTGATAAAATGCATTATCTTCTCCATTTTACAATGGCTTTCTTATCCTTCCTATATTGTGGGTTGTTGCCTCCATTGGTTTATGCATTTTCATTTGCTAAAACTGGCAACAAGGATCTCAAGATGATAGCCACTGCAGGGGCTTCTCTTTCATGCACTGCAATTCTTGCTCTTCAAAAAGCTCACATCCAAAAACCACAAAATTGTCAAGTGTACATCAAAACTGCAACCACACATGTCTCTGTTGGACTTGTAGCTTTTGGCTTATCTTACCTTTCAGGTTATATGATTGGACAGCTCACAGACAGGCTTGTTTGGAGCTAA

mRNA sequence

ATGAAGATTTTTCTAAACTCATTAGAAGAAGTAAACAAAGACGTTGGGTCTTCAGAATCAGTAGTTGTCAACATTCCTCTTGGGAAAAGAGAAACAACTCTCCGACAGAATAGCAATGGAGCTAGAAAGTGGGAAGTGCAGAAAGCCATAGTAGTTGGTGGTTTATTTGAATCAATCACAAGTTTAGTCATTGTAACCGCTGCAGCAAGTGTTAATATTTCCATTGAAAATATTGTAGCTTTGGCAATGACAAACTTGATTAGTGGACTTTTTGTCGTTACACATAATCTATCAGAACTGAAGATCAAGAATAGGCTTGAGAAGGAATATAATGGAGTTGAAGTAGAAGGAATACAGTATAAAGAAGTATTGATTGATAAAATGCATTATCTTCTCCATTTTACAATGGCTTTCTTATCCTTCCTATATTGTGGGTTGTTGCCTCCATTGGTTTATGCATTTTCATTTGCTAAAACTGGCAACAAGGATCTCAAGATGATAGCCACTGCAGGGGCTTCTCTTTCATGCACTGCAATTCTTGCTCTTCAAAAAGCTCACATCCAAAAACCACAAAATTGTCAAGTGTACATCAAAACTGCAACCACACATGTCTCTGTTGGACTTGTAGCTTTTGGCTTATCTTACCTTTCAGGTTATATGATTGGACAGCTCACAGACAGGCTTGTTTGGAGCTAA

Coding sequence (CDS)

ATGAAGATTTTTCTAAACTCATTAGAAGAAGTAAACAAAGACGTTGGGTCTTCAGAATCAGTAGTTGTCAACATTCCTCTTGGGAAAAGAGAAACAACTCTCCGACAGAATAGCAATGGAGCTAGAAAGTGGGAAGTGCAGAAAGCCATAGTAGTTGGTGGTTTATTTGAATCAATCACAAGTTTAGTCATTGTAACCGCTGCAGCAAGTGTTAATATTTCCATTGAAAATATTGTAGCTTTGGCAATGACAAACTTGATTAGTGGACTTTTTGTCGTTACACATAATCTATCAGAACTGAAGATCAAGAATAGGCTTGAGAAGGAATATAATGGAGTTGAAGTAGAAGGAATACAGTATAAAGAAGTATTGATTGATAAAATGCATTATCTTCTCCATTTTACAATGGCTTTCTTATCCTTCCTATATTGTGGGTTGTTGCCTCCATTGGTTTATGCATTTTCATTTGCTAAAACTGGCAACAAGGATCTCAAGATGATAGCCACTGCAGGGGCTTCTCTTTCATGCACTGCAATTCTTGCTCTTCAAAAAGCTCACATCCAAAAACCACAAAATTGTCAAGTGTACATCAAAACTGCAACCACACATGTCTCTGTTGGACTTGTAGCTTTTGGCTTATCTTACCTTTCAGGTTATATGATTGGACAGCTCACAGACAGGCTTGTTTGGAGCTAA

Protein sequence

MKIFLNSLEEVNKDVGSSESVVVNIPLGKRETTLRQNSNGARKWEVQKAIVVGGLFESITSLVIVTAAASVNISIENIVALAMTNLISGLFVVTHNLSELKIKNRLEKEYNGVEVEGIQYKEVLIDKMHYLLHFTMAFLSFLYCGLLPPLVYAFSFAKTGNKDLKMIATAGASLSCTAILALQKAHIQKPQNCQVYIKTATTHVSVGLVAFGLSYLSGYMIGQLTDRLVWS

Homology

[Close]

BLAST of Lag0028148 vs. NCBI nr
Match: XP_023548918.1 (uncharacterized protein LOC111807425 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 207.2 bits (526), Expect = 1.5e-49
Identity = 113/194 (58.25%), Postives = 150/194 (77.32%), Query Frame = 0


Query: 38  SNGARKWEVQKAIVVGGLFESITSLVIVTAAASVNISIENIVALAMTNLISGLFVVTHNL 97
           S   R+WE+++ IV+GGL ESITSL IVTAA S  IS  NI+ALA+TNLI+GLF++ H++
Sbjct: 750 STRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIIALALTNLIAGLFIIRHDV 809

Query: 98  SELKIKNRLEKEYNGVEVEGIQYKEVLIDKMHYLLHFTMAFLSFLYCGLLPPLVYAFSFA 157
           S L+ K ++ +    +EV+G  YKE+L  KM+ LL F +A+LSFL+ GL+PPLVYA S  
Sbjct: 810 SRLQKKRKIPR----IEVDGNYYKEILKGKMYSLLCFIIAYLSFLFFGLVPPLVYALSSL 869

Query: 158 KTGNKDLKMIATAGASLSCTAILALQKAHI-QKPQNCQVYIKTATTHVSVGLVAFGLSYL 217
           K  NK+LK+IA AGASLSCTA+LA+QKAHI QKP+N  VY+KTA  +VSVG+ AFG+SYL
Sbjct: 870 KIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRNRLVYVKTAGVYVSVGVGAFGISYL 929

Query: 218 SGYMIGQLTDRLVW 231
           +GY+ G L + L+W
Sbjct: 930 AGYIFGHLAEELLW 939

[Close]

BLAST of Lag0028148 vs. NCBI nr
Match: XP_023548919.1 (uncharacterized protein LOC111807425 isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 207.2 bits (526), Expect = 1.5e-49
Identity = 113/194 (58.25%), Postives = 150/194 (77.32%), Query Frame = 0


Query: 38  SNGARKWEVQKAIVVGGLFESITSLVIVTAAASVNISIENIVALAMTNLISGLFVVTHNL 97
           S   R+WE+++ IV+GGL ESITSL IVTAA S  IS  NI+ALA+TNLI+GLF++ H++
Sbjct: 749 STRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIIALALTNLIAGLFIIRHDV 808

Query: 98  SELKIKNRLEKEYNGVEVEGIQYKEVLIDKMHYLLHFTMAFLSFLYCGLLPPLVYAFSFA 157
           S L+ K ++ +    +EV+G  YKE+L  KM+ LL F +A+LSFL+ GL+PPLVYA S  
Sbjct: 809 SRLQKKRKIPR----IEVDGNYYKEILKGKMYSLLCFIIAYLSFLFFGLVPPLVYALSSL 868

Query: 158 KTGNKDLKMIATAGASLSCTAILALQKAHI-QKPQNCQVYIKTATTHVSVGLVAFGLSYL 217
           K  NK+LK+IA AGASLSCTA+LA+QKAHI QKP+N  VY+KTA  +VSVG+ AFG+SYL
Sbjct: 869 KIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRNRLVYVKTAGVYVSVGVGAFGISYL 928

Query: 218 SGYMIGQLTDRLVW 231
           +GY+ G L + L+W
Sbjct: 929 AGYIFGHLAEELLW 938

[Close]

BLAST of Lag0028148 vs. NCBI nr
Match: XP_023548916.1 (uncharacterized protein LOC111807425 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023548917.1 uncharacterized protein LOC111807425 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 207.2 bits (526), Expect = 1.5e-49
Identity = 113/194 (58.25%), Postives = 150/194 (77.32%), Query Frame = 0


Query: 38  SNGARKWEVQKAIVVGGLFESITSLVIVTAAASVNISIENIVALAMTNLISGLFVVTHNL 97
           S   R+WE+++ IV+GGL ESITSL IVTAA S  IS  NI+ALA+TNLI+GLF++ H++
Sbjct: 755 STRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIIALALTNLIAGLFIIRHDV 814

Query: 98  SELKIKNRLEKEYNGVEVEGIQYKEVLIDKMHYLLHFTMAFLSFLYCGLLPPLVYAFSFA 157
           S L+ K ++ +    +EV+G  YKE+L  KM+ LL F +A+LSFL+ GL+PPLVYA S  
Sbjct: 815 SRLQKKRKIPR----IEVDGNYYKEILKGKMYSLLCFIIAYLSFLFFGLVPPLVYALSSL 874

Query: 158 KTGNKDLKMIATAGASLSCTAILALQKAHI-QKPQNCQVYIKTATTHVSVGLVAFGLSYL 217
           K  NK+LK+IA AGASLSCTA+LA+QKAHI QKP+N  VY+KTA  +VSVG+ AFG+SYL
Sbjct: 875 KIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRNRLVYVKTAGVYVSVGVGAFGISYL 934

Query: 218 SGYMIGQLTDRLVW 231
           +GY+ G L + L+W
Sbjct: 935 AGYIFGHLAEELLW 944

[Close]

BLAST of Lag0028148 vs. NCBI nr
Match: XP_023548920.1 (uncharacterized protein LOC111807425 isoform X5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 207.2 bits (526), Expect = 1.5e-49
Identity = 113/194 (58.25%), Postives = 150/194 (77.32%), Query Frame = 0


Query: 38  SNGARKWEVQKAIVVGGLFESITSLVIVTAAASVNISIENIVALAMTNLISGLFVVTHNL 97
           S   R+WE+++ IV+GGL ESITSL IVTAA S  IS  NI+ALA+TNLI+GLF++ H++
Sbjct: 717 STRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIIALALTNLIAGLFIIRHDV 776

Query: 98  SELKIKNRLEKEYNGVEVEGIQYKEVLIDKMHYLLHFTMAFLSFLYCGLLPPLVYAFSFA 157
           S L+ K ++ +    +EV+G  YKE+L  KM+ LL F +A+LSFL+ GL+PPLVYA S  
Sbjct: 777 SRLQKKRKIPR----IEVDGNYYKEILKGKMYSLLCFIIAYLSFLFFGLVPPLVYALSSL 836

Query: 158 KTGNKDLKMIATAGASLSCTAILALQKAHI-QKPQNCQVYIKTATTHVSVGLVAFGLSYL 217
           K  NK+LK+IA AGASLSCTA+LA+QKAHI QKP+N  VY+KTA  +VSVG+ AFG+SYL
Sbjct: 837 KIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRNRLVYVKTAGVYVSVGVGAFGISYL 896

Query: 218 SGYMIGQLTDRLVW 231
           +GY+ G L + L+W
Sbjct: 897 AGYIFGHLAEELLW 906

[Close]

BLAST of Lag0028148 vs. NCBI nr
Match: XP_023548921.1 (uncharacterized protein LOC111807425 isoform X6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 207.2 bits (526), Expect = 1.5e-49
Identity = 113/194 (58.25%), Postives = 150/194 (77.32%), Query Frame = 0


Query: 38  SNGARKWEVQKAIVVGGLFESITSLVIVTAAASVNISIENIVALAMTNLISGLFVVTHNL 97
           S   R+WE+++ IV+GGL ESITSL IVTAA S  IS  NI+ALA+TNLI+GLF++ H++
Sbjct: 711 STRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIIALALTNLIAGLFIIRHDV 770

Query: 98  SELKIKNRLEKEYNGVEVEGIQYKEVLIDKMHYLLHFTMAFLSFLYCGLLPPLVYAFSFA 157
           S L+ K ++ +    +EV+G  YKE+L  KM+ LL F +A+LSFL+ GL+PPLVYA S  
Sbjct: 771 SRLQKKRKIPR----IEVDGNYYKEILKGKMYSLLCFIIAYLSFLFFGLVPPLVYALSSL 830

Query: 158 KTGNKDLKMIATAGASLSCTAILALQKAHI-QKPQNCQVYIKTATTHVSVGLVAFGLSYL 217
           K  NK+LK+IA AGASLSCTA+LA+QKAHI QKP+N  VY+KTA  +VSVG+ AFG+SYL
Sbjct: 831 KIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRNRLVYVKTAGVYVSVGVGAFGISYL 890

Query: 218 SGYMIGQLTDRLVW 231
           +GY+ G L + L+W
Sbjct: 891 AGYIFGHLAEELLW 900

[Close]

BLAST of Lag0028148 vs. ExPASy Swiss-Prot
Match: Q8LPT3 (Membrane protein of ER body-like protein OS=Arabidopsis thaliana OX=3702 GN=MEBL PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.5e-31
Identity = 81/207 (39.13%), Postives = 129/207 (62.32%), Query Frame = 0


Query: 30  RETTLRQNSNGARKWEVQKAIVVGGLFESITSLVIVTAAASVNISIENIVALAMTNLISG 89
           RE  +R+     RK E+ K+IV GGL E+ITSL ++++AA    S+ NI+ L + NL+ G
Sbjct: 529 REPLMRRVVVQGRKLEILKSIVYGGLLEAITSLGVISSAAGSGASMLNILVLGLANLLGG 588

Query: 90  LFVVTHNLSELKIKNRL-----EKEYNGVEVEGIQYKEVLIDKMHYLLHFTMAFLSFLYC 149
           L ++ HNL EL+ +  +     + + NG E E  +YK +L  + ++ LH T+A LSF+  
Sbjct: 589 LILIIHNLQELREEEPIRTTTEDNQTNGREEEEGRYKRLLGRRENFTLHATVAILSFIIT 648

Query: 150 GLLPPLVYAFSFAKTGNKDLKMIATAGASLSCTAILALQKAHIQKPQNCQVYIKTATTHV 209
           G+LPP+VY FSF++  NKD K+ +  GASL C  +LA+ KAH++ P+    Y+K+   + 
Sbjct: 649 GILPPVVYYFSFSEKHNKDYKVASVFGASLFCIVLLAIAKAHVRYPRGS--YLKSILYYG 708

Query: 210 SVGLVAFGLSYLSGYMIGQLTDRLVWS 232
           S+ +   G+SY+ G  + QL ++  WS
Sbjct: 709 SIAVSVSGISYVVGNFLEQLLEKHGWS 733

[Close]

BLAST of Lag0028148 vs. ExPASy Swiss-Prot
Match: F4KFS7 (Membrane protein of ER body 2 OS=Arabidopsis thaliana OX=3702 GN=MEB2 PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-23
Identity = 68/188 (36.17%), Postives = 116/188 (61.70%), Query Frame = 0


Query: 45  EVQKAIVVGGLFESITSLVIVTAAASVNISIENIVALAMTNLISGLFVVTHNLSELKIKN 104
           E+ K+ V GGL E+ITSL +V++A++   S  NI+ALA+ NL  GL V+  N  +L+  +
Sbjct: 345 ELLKSTVYGGLTETITSLGVVSSASASGSSTMNILALAVANLAGGLIVLAQNFQDLRNSS 404

Query: 105 RLEKEYNGVEVEGIQYKEVLIDKMHYLLHFTMAFLSFLYCGLLPPLVYAFSFAKTGNKDL 164
             EK+         +Y+E+L  +    +H  +A +S+++ GL+PPLVYAFSF +TG K+ 
Sbjct: 405 DQEKD---------RYEELLGRRTKSRIHILVAVMSYIFFGLIPPLVYAFSFYETGIKNY 464

Query: 165 KMIATAGASLSCTAILALQKAHIQKPQN----CQVYIKTATTHVSVGLVAFGLSYLSGYM 224
           K+I+    SL C  +L   K +++KP N     + Y+K+A  + S+ + + G+SY+ G +
Sbjct: 465 KLISVFLGSLVCVILLGSIKVYVRKPTNSCGSTKAYLKSAAYYTSIVVASCGISYVVGDI 523

Query: 225 IGQLTDRL 229
           +G+  ++L
Sbjct: 525 MGEYIEKL 523

[Close]

BLAST of Lag0028148 vs. ExPASy Swiss-Prot
Match: Q8W4P8 (Membrane protein of ER body 1 OS=Arabidopsis thaliana OX=3702 GN=MEB1 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 5.6e-23
Identity = 74/195 (37.95%), Postives = 112/195 (57.44%), Query Frame = 0


Query: 40  GARKWEVQKAIVVGGLFESITSLVIVTAAASVNISIENIVALAMTNLISGLFVVTHNLSE 99
           G  K E+ K+IV GGL ESITSL  VT+AA+   S  N++AL + NL SGL +  H+L E
Sbjct: 407 GGNKVEILKSIVYGGLTESITSLCTVTSAAASGASTLNVLALGVANLSSGLLLTVHSLQE 466

Query: 100 L---KIKNRLEKEYNGVEVEGIQ--YKEVLIDKMHYLLHFTMAFLSFLYCGLLPPLVYAF 159
           L   K + +   + +  E EG +  Y+EVL  + +  +H  +A  SF+  GL+PPLVY F
Sbjct: 467 LINEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIFGLIPPLVYGF 526

Query: 160 SFAKTGNK--DLKMIATAGASLSCTAILALQKAHIQKPQNCQVYIKTATTHVSVGLVAFG 219
           SF K   K  + K++A    SL C  +L++ KA++ K ++   Y+KT   + +    A G
Sbjct: 527 SFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKKRD---YVKTLFRYTTTATTASG 586

Query: 220 LSYLSGYMIGQLTDR 228
            S   GY++ Q  ++
Sbjct: 587 FSQFVGYLVSQWLEK 598

[Close]

BLAST of Lag0028148 vs. ExPASy TrEMBL
Match: A0A6J1GQF8 (uncharacterized protein LOC111456533 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456533 PE=3 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 1.2e-49
Identity = 114/194 (58.76%), Postives = 149/194 (76.80%), Query Frame = 0


Query: 38  SNGARKWEVQKAIVVGGLFESITSLVIVTAAASVNISIENIVALAMTNLISGLFVVTHNL 97
           S   R+WE+++ IV+GGL ESITSL IVTAA S  IS  NI+ALA+TNLI+GLF++ H++
Sbjct: 719 STRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIIALALTNLIAGLFIIRHDV 778

Query: 98  SELKIKNRLEKEYNGVEVEGIQYKEVLIDKMHYLLHFTMAFLSFLYCGLLPPLVYAFSFA 157
           S L+ K ++ +    +EV+G  YKEVL +KM+ LL F +A+LSFL  GL+PPLVYA S  
Sbjct: 779 SRLQKKRKIPR----IEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSL 838

Query: 158 KTGNKDLKMIATAGASLSCTAILALQKAHI-QKPQNCQVYIKTATTHVSVGLVAFGLSYL 217
           K  NK+LK+IA AGASLSCTA+LA+QKAHI QKP++  VY+KTA   VSVG+ AFG+SYL
Sbjct: 839 KIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYL 898

Query: 218 SGYMIGQLTDRLVW 231
           +GYM G L + L+W
Sbjct: 899 AGYMFGHLAEELLW 908

[Close]

BLAST of Lag0028148 vs. ExPASy TrEMBL
Match: A0A6J1GQA4 (uncharacterized protein LOC111456533 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456533 PE=3 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 1.2e-49
Identity = 114/194 (58.76%), Postives = 149/194 (76.80%), Query Frame = 0


Query: 38  SNGARKWEVQKAIVVGGLFESITSLVIVTAAASVNISIENIVALAMTNLISGLFVVTHNL 97
           S   R+WE+++ IV+GGL ESITSL IVTAA S  IS  NI+ALA+TNLI+GLF++ H++
Sbjct: 720 STRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIIALALTNLIAGLFIIRHDV 779

Query: 98  SELKIKNRLEKEYNGVEVEGIQYKEVLIDKMHYLLHFTMAFLSFLYCGLLPPLVYAFSFA 157
           S L+ K ++ +    +EV+G  YKEVL +KM+ LL F +A+LSFL  GL+PPLVYA S  
Sbjct: 780 SRLQKKRKIPR----IEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSL 839

Query: 158 KTGNKDLKMIATAGASLSCTAILALQKAHI-QKPQNCQVYIKTATTHVSVGLVAFGLSYL 217
           K  NK+LK+IA AGASLSCTA+LA+QKAHI QKP++  VY+KTA   VSVG+ AFG+SYL
Sbjct: 840 KIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYL 899

Query: 218 SGYMIGQLTDRLVW 231
           +GYM G L + L+W
Sbjct: 900 AGYMFGHLAEELLW 909

[Close]

BLAST of Lag0028148 vs. ExPASy TrEMBL
Match: A0A6J1GQF7 (uncharacterized protein LOC111456533 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111456533 PE=3 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 1.2e-49
Identity = 114/194 (58.76%), Postives = 149/194 (76.80%), Query Frame = 0


Query: 38  SNGARKWEVQKAIVVGGLFESITSLVIVTAAASVNISIENIVALAMTNLISGLFVVTHNL 97
           S   R+WE+++ IV+GGL ESITSL IVTAA S  IS  NI+ALA+TNLI+GLF++ H++
Sbjct: 532 STRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIIALALTNLIAGLFIIRHDV 591

Query: 98  SELKIKNRLEKEYNGVEVEGIQYKEVLIDKMHYLLHFTMAFLSFLYCGLLPPLVYAFSFA 157
           S L+ K ++ +    +EV+G  YKEVL +KM+ LL F +A+LSFL  GL+PPLVYA S  
Sbjct: 592 SRLQKKRKIPR----IEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSL 651

Query: 158 KTGNKDLKMIATAGASLSCTAILALQKAHI-QKPQNCQVYIKTATTHVSVGLVAFGLSYL 217
           K  NK+LK+IA AGASLSCTA+LA+QKAHI QKP++  VY+KTA   VSVG+ AFG+SYL
Sbjct: 652 KIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYL 711

Query: 218 SGYMIGQLTDRLVW 231
           +GYM G L + L+W
Sbjct: 712 AGYMFGHLAEELLW 721

[Close]

BLAST of Lag0028148 vs. ExPASy TrEMBL
Match: A0A6J1JQG4 (membrane protein of ER body 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488012 PE=3 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 2.8e-46
Identity = 115/207 (55.56%), Postives = 151/207 (72.95%), Query Frame = 0


Query: 19  ESVVVNIPLGKRETTLRQNSNGARKWEVQKAIVVGGLFESITSLVIVTAAASVNISIENI 78
           E + + IP  +  T +R      R+WE+++ IV+GGL ESITSL IVTAA +  IS  NI
Sbjct: 5   ECLAIMIPPYEPSTRVR------RRWEIRRYIVLGGLMESITSLAIVTAAMTAKISDGNI 64

Query: 79  VALAMTNLISGLFVVTHNLSELKIKNRLEKEYNGVEVEGIQYKEVLIDKMHYLLHFTMAF 138
           VALA+TNL++GLFV+ H++S L+ K ++ +    +EV+G  YKEVL +KM+ LL F +A+
Sbjct: 65  VALALTNLLAGLFVIRHDVSRLQKKRKIPR----IEVDGNNYKEVLKEKMYRLLCFIIAY 124

Query: 139 LSFLYCGLLPPLVYAFSFAKTGNKDLKMIATAGASLSCTAILALQKAHI-QKPQNCQVYI 198
           LSFL+ GL+PPLVYA S  K  NK+LK+I  AGASLSCTA+LA+QKAHI QKP+N  VY+
Sbjct: 125 LSFLFFGLVPPLVYALSSLKIRNKNLKIIVAAGASLSCTALLAVQKAHINQKPRNRLVYV 184

Query: 199 KTATTHVSVGLVAFGLSYLSGYMIGQL 225
           KTA   VSV + AFG+SYL GYM G L
Sbjct: 185 KTAAVDVSVRVGAFGISYLVGYMFGTL 201

[Close]

BLAST of Lag0028148 vs. ExPASy TrEMBL
Match: A0A5D3CJ51 (Membrane protein of ER body-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold263G00390 PE=3 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 6.6e-43
Identity = 114/220 (51.82%), Postives = 147/220 (66.82%), Query Frame = 0


Query: 14  DVGSSESVVVNIPLGKRETTLRQNSNGARKWEVQKAIVVGGLFESITSLVIVTAAASVNI 73
           +V   +S+V+ IP    E TL Q   G R+ E+ K+IV GGL E+ITSL IV +AAS + 
Sbjct: 276 EVEGGDSIVLTIPPVSGEETLGQ--RGGRRVEIMKSIVYGGLTEAITSLGIVASAASAST 335

Query: 74  SIENIVALAMTNLISGLFVVTHNLSELKIKNRLEKEYNGVEVEGIQ---YKEVLIDKMHY 133
             ENIVALA+ NLI+GL V+T+NLS LK  N+L+KE N  +   +Q   Y+E L D+ HY
Sbjct: 336 PTENIVALALANLITGLIVITNNLSGLK-SNQLKKESNQNDDGAVQVDPYEEALGDRHHY 395

Query: 134 LLHFTMAFLSFLYCGLLPPLVYAFSFAKTGNKDLKMIATAGASLSCTAILALQKAHIQKP 193
           LLHFT A LSFL  GLLPPLVY FSF  T + DLK+ A A +SL C A+LA+ KA++QK 
Sbjct: 396 LLHFTTAILSFLIFGLLPPLVYGFSFRDTDDGDLKLAAVAVSSLLCIALLAIVKAYVQKA 455

Query: 194 QNCQVYIKTATTHVSVGLVAFGLSYLSGYMIGQLTDRLVW 231
            N Q Y KT   +VS+G  A GLSYL+G  +  L ++L W
Sbjct: 456 NNLQEYAKTLVYYVSLGFGASGLSYLAGKEVNILLEQLGW 492

[Close]

BLAST of Lag0028148 vs. TAIR 10
Match: AT4G27870.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 137.9 bits (346), Expect = 1.0e-32
Identity = 81/207 (39.13%), Postives = 129/207 (62.32%), Query Frame = 0


Query: 30  RETTLRQNSNGARKWEVQKAIVVGGLFESITSLVIVTAAASVNISIENIVALAMTNLISG 89
           RE  +R+     RK E+ K+IV GGL E+ITSL ++++AA    S+ NI+ L + NL+ G
Sbjct: 529 REPLMRRVVVQGRKLEILKSIVYGGLLEAITSLGVISSAAGSGASMLNILVLGLANLLGG 588

Query: 90  LFVVTHNLSELKIKNRL-----EKEYNGVEVEGIQYKEVLIDKMHYLLHFTMAFLSFLYC 149
           L ++ HNL EL+ +  +     + + NG E E  +YK +L  + ++ LH T+A LSF+  
Sbjct: 589 LILIIHNLQELREEEPIRTTTEDNQTNGREEEEGRYKRLLGRRENFTLHATVAILSFIIT 648

Query: 150 GLLPPLVYAFSFAKTGNKDLKMIATAGASLSCTAILALQKAHIQKPQNCQVYIKTATTHV 209
           G+LPP+VY FSF++  NKD K+ +  GASL C  +LA+ KAH++ P+    Y+K+   + 
Sbjct: 649 GILPPVVYYFSFSEKHNKDYKVASVFGASLFCIVLLAIAKAHVRYPRGS--YLKSILYYG 708

Query: 210 SVGLVAFGLSYLSGYMIGQLTDRLVWS 232
           S+ +   G+SY+ G  + QL ++  WS
Sbjct: 709 SIAVSVSGISYVVGNFLEQLLEKHGWS 733

[Close]

BLAST of Lag0028148 vs. TAIR 10
Match: AT5G24290.2 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 111.7 bits (278), Expect = 8.0e-25
Identity = 68/188 (36.17%), Postives = 116/188 (61.70%), Query Frame = 0


Query: 45  EVQKAIVVGGLFESITSLVIVTAAASVNISIENIVALAMTNLISGLFVVTHNLSELKIKN 104
           E+ K+ V GGL E+ITSL +V++A++   S  NI+ALA+ NL  GL V+  N  +L+  +
Sbjct: 329 ELLKSTVYGGLTETITSLGVVSSASASGSSTMNILALAVANLAGGLIVLAQNFQDLRNSS 388

Query: 105 RLEKEYNGVEVEGIQYKEVLIDKMHYLLHFTMAFLSFLYCGLLPPLVYAFSFAKTGNKDL 164
             EK+         +Y+E+L  +    +H  +A +S+++ GL+PPLVYAFSF +TG K+ 
Sbjct: 389 DQEKD---------RYEELLGRRTKSRIHILVAVMSYIFFGLIPPLVYAFSFYETGIKNY 448

Query: 165 KMIATAGASLSCTAILALQKAHIQKPQN----CQVYIKTATTHVSVGLVAFGLSYLSGYM 224
           K+I+    SL C  +L   K +++KP N     + Y+K+A  + S+ + + G+SY+ G +
Sbjct: 449 KLISVFLGSLVCVILLGSIKVYVRKPTNSCGSTKAYLKSAAYYTSIVVASCGISYVVGDI 507

Query: 225 IGQLTDRL 229
           +G+  ++L
Sbjct: 509 MGEYIEKL 507

[Close]

BLAST of Lag0028148 vs. TAIR 10
Match: AT5G24290.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 111.7 bits (278), Expect = 8.0e-25
Identity = 68/188 (36.17%), Postives = 116/188 (61.70%), Query Frame = 0


Query: 45  EVQKAIVVGGLFESITSLVIVTAAASVNISIENIVALAMTNLISGLFVVTHNLSELKIKN 104
           E+ K+ V GGL E+ITSL +V++A++   S  NI+ALA+ NL  GL V+  N  +L+  +
Sbjct: 345 ELLKSTVYGGLTETITSLGVVSSASASGSSTMNILALAVANLAGGLIVLAQNFQDLRNSS 404

Query: 105 RLEKEYNGVEVEGIQYKEVLIDKMHYLLHFTMAFLSFLYCGLLPPLVYAFSFAKTGNKDL 164
             EK+         +Y+E+L  +    +H  +A +S+++ GL+PPLVYAFSF +TG K+ 
Sbjct: 405 DQEKD---------RYEELLGRRTKSRIHILVAVMSYIFFGLIPPLVYAFSFYETGIKNY 464

Query: 165 KMIATAGASLSCTAILALQKAHIQKPQN----CQVYIKTATTHVSVGLVAFGLSYLSGYM 224
           K+I+    SL C  +L   K +++KP N     + Y+K+A  + S+ + + G+SY+ G +
Sbjct: 465 KLISVFLGSLVCVILLGSIKVYVRKPTNSCGSTKAYLKSAAYYTSIVVASCGISYVVGDI 523

Query: 225 IGQLTDRL 229
           +G+  ++L
Sbjct: 525 MGEYIEKL 523

[Close]

BLAST of Lag0028148 vs. TAIR 10
Match: AT4G27860.1 (vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 109.4 bits (272), Expect = 4.0e-24
Identity = 74/195 (37.95%), Postives = 112/195 (57.44%), Query Frame = 0


Query: 40  GARKWEVQKAIVVGGLFESITSLVIVTAAASVNISIENIVALAMTNLISGLFVVTHNLSE 99
           G  K E+ K+IV GGL ESITSL  VT+AA+   S  N++AL + NL SGL +  H+L E
Sbjct: 407 GGNKVEILKSIVYGGLTESITSLCTVTSAAASGASTLNVLALGVANLSSGLLLTVHSLQE 466

Query: 100 L---KIKNRLEKEYNGVEVEGIQ--YKEVLIDKMHYLLHFTMAFLSFLYCGLLPPLVYAF 159
           L   K + +   + +  E EG +  Y+EVL  + +  +H  +A  SF+  GL+PPLVY F
Sbjct: 467 LINEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIFGLIPPLVYGF 526

Query: 160 SFAKTGNK--DLKMIATAGASLSCTAILALQKAHIQKPQNCQVYIKTATTHVSVGLVAFG 219
           SF K   K  + K++A    SL C  +L++ KA++ K ++   Y+KT   + +    A G
Sbjct: 527 SFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKKRD---YVKTLFRYTTTATTASG 586

Query: 220 LSYLSGYMIGQLTDR 228
            S   GY++ Q  ++
Sbjct: 587 FSQFVGYLVSQWLEK 598

[Close]

BLAST of Lag0028148 vs. TAIR 10
Match: AT4G27860.2 (vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 109.4 bits (272), Expect = 4.0e-24
Identity = 74/195 (37.95%), Postives = 112/195 (57.44%), Query Frame = 0


Query: 40  GARKWEVQKAIVVGGLFESITSLVIVTAAASVNISIENIVALAMTNLISGLFVVTHNLSE 99
           G  K E+ K+IV GGL ESITSL  VT+AA+   S  N++AL + NL SGL +  H+L E
Sbjct: 392 GGNKVEILKSIVYGGLTESITSLCTVTSAAASGASTLNVLALGVANLSSGLLLTVHSLQE 451

Query: 100 L---KIKNRLEKEYNGVEVEGIQ--YKEVLIDKMHYLLHFTMAFLSFLYCGLLPPLVYAF 159
           L   K + +   + +  E EG +  Y+EVL  + +  +H  +A  SF+  GL+PPLVY F
Sbjct: 452 LINEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIFGLIPPLVYGF 511

Query: 160 SFAKTGNK--DLKMIATAGASLSCTAILALQKAHIQKPQNCQVYIKTATTHVSVGLVAFG 219
           SF K   K  + K++A    SL C  +L++ KA++ K ++   Y+KT   + +    A G
Sbjct: 512 SFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKKRD---YVKTLFRYTTTATTASG 571

Query: 220 LSYLSGYMIGQLTDR 228
            S   GY++ Q  ++
Sbjct: 572 FSQFVGYLVSQWLEK 583

The following BLAST results are available for this feature:

BLAST of Lag0028148 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023548918.1	1.5e-49	58.25	uncharacterized protein LOC111807425 isoform X3 [Cucurbita pepo subsp. pepo]	[more]
XP_023548919.1	1.5e-49	58.25	uncharacterized protein LOC111807425 isoform X4 [Cucurbita pepo subsp. pepo]	[more]
XP_023548916.1	1.5e-49	58.25	uncharacterized protein LOC111807425 isoform X1 [Cucurbita pepo subsp. pepo] >XP...	[more]
XP_023548920.1	1.5e-49	58.25	uncharacterized protein LOC111807425 isoform X5 [Cucurbita pepo subsp. pepo]	[more]
XP_023548921.1	1.5e-49	58.25	uncharacterized protein LOC111807425 isoform X6 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0028148 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPT3	1.5e-31	39.13	Membrane protein of ER body-like protein OS=Arabidopsis thaliana OX=3702 GN=MEBL...	[more]
F4KFS7	1.1e-23	36.17	Membrane protein of ER body 2 OS=Arabidopsis thaliana OX=3702 GN=MEB2 PE=1 SV=1	[more]
Q8W4P8	5.6e-23	37.95	Membrane protein of ER body 1 OS=Arabidopsis thaliana OX=3702 GN=MEB1 PE=1 SV=1	[more]

BLAST of Lag0028148 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GQF8	1.2e-49	58.76	uncharacterized protein LOC111456533 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1GQA4	1.2e-49	58.76	uncharacterized protein LOC111456533 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1GQF7	1.2e-49	58.76	uncharacterized protein LOC111456533 isoform X3 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1JQG4	2.8e-46	55.56	membrane protein of ER body 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488012 P...	[more]
A0A5D3CJ51	6.6e-43	51.82	Membrane protein of ER body-like protein OS=Cucumis melo var. makuwa OX=1194695 ...	[more]

BLAST of Lag0028148 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27870.1	1.0e-32	39.13	Vacuolar iron transporter (VIT) family protein	[more]
AT5G24290.2	8.0e-25	36.17	Vacuolar iron transporter (VIT) family protein	[more]
AT5G24290.1	8.0e-25	36.17	Vacuolar iron transporter (VIT) family protein	[more]
AT4G27860.1	4.0e-24	37.95	vacuolar iron transporter (VIT) family protein	[more]
AT4G27860.2	4.0e-24	37.95	vacuolar iron transporter (VIT) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008217	Ccc1 family	PANTHER	PTHR31851	FE(2+)/MN(2+) TRANSPORTER PCL1	coord: 14..230
None	No IPR available	PANTHER	PTHR31851:SF64	GLYCINE-RICH PROTEIN FAMILY	coord: 14..230

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0028148.1	Lag0028148.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030026	cellular manganese ion homeostasis
biological_process	GO:0098662	inorganic cation transmembrane transport
biological_process	GO:0000041	transition metal ion transport
cellular_component	GO:0016020	membrane
molecular_function	GO:0005384	manganese ion transmembrane transporter activity