Lag0028102 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0028102
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	ribonuclease MC-like
Location	chr8: 13070920 .. 13071903 (+)
RNA-Seq Expression	Lag0028102
Synteny	Lag0028102

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAAGAAAGAACTTGTATTGATTTATAGTATGTTAGTACTGATAGCAAGTTGCGAGGGACTCAATGAGTATGACTACTTGCAAGTTGTGATTCAATGGCAACCAGCCACGTGTAGTGATTGGAACAAACCATATCCTTATTACTTTCAAGCTCCAGATAATCGATTCAGTATCCATGGCATGTGGCCCAGTCTATACAATGGTGGAGAAACCCAATGTTCTGGCGAGCCATTCGATGTAAACAAGGTAATTAATTAAGTGTGCGTTTGGAATACCTTTTAATTTAAGGATGCATTTTCTTTTAAACATTTTGTATAAAGATACGTTTTCTTCTAATAACATATAGTCACATGTCTAAATGCGTTAACAAAACAATATAATTTATCAACTACAAATTTTGAACATAAATCAGGAATTACTTAGAATACCGAGACTCTGGTCAACTCATTGACCCTAAAAGTATTACATGAGTTGTTTTGCTCATGATATGATATATGAAAATTACTCGATGGATGTTTATTGATTCTCCAGATTAGCACACTACAACCTGGAATTAGTGAAATCTGGCCAAATGTTGCCACAGGAAACAACCTATGGCTATGGGGTCACGAGTGGGATAAGCATGGAACCTGCACAGAAGCCATTTTATTTGACCAAGAAGGATATTTCAGTTTTGCAATGGACAAATATATTGAAAACAATTTGTTGTTTATTCTTGGCAATAGCCAAATTACACCTAACGGACAAGTATACTATTTAAGAAATCAGCTTTACGCGGCAATAAGAGCAAAAACTGGAAGAACACCAGCTGTTCGATGCAACTACAACATTTGGAGTGAGGTACAACAATTGCATGAAATAACTCTGTGTTATAATTGGAATGCTACTGCTTTTGTGGACTGCCCTTTGAACGCCAACAAATGCGATAATTACTTTGTGTGGTATCCGTGGAACACTGCCAAGGTGTTGTCTAAAGAATGA

mRNA sequence

ATGGCGAAGAAAGAACTTGTATTGATTTATAGTATGTTAGTACTGATAGCAAGTTGCGAGGGACTCAATGAGTATGACTACTTGCAAGTTGTGATTCAATGGCAACCAGCCACGTGTAGTGATTGGAACAAACCATATCCTTATTACTTTCAAGCTCCAGATAATCGATTCAGTATCCATGGCATGTGGCCCAGTCTATACAATGGTGGAGAAACCCAATGTTCTGGCGAGCCATTCGATGTAAACAAGATTAGCACACTACAACCTGGAATTAGTGAAATCTGGCCAAATGTTGCCACAGGAAACAACCTATGGCTATGGGGTCACGAGTGGGATAAGCATGGAACCTGCACAGAAGCCATTTTATTTGACCAAGAAGGATATTTCAGTTTTGCAATGGACAAATATATTGAAAACAATTTGTTGTTTATTCTTGGCAATAGCCAAATTACACCTAACGGACAAGTATACTATTTAAGAAATCAGCTTTACGCGGCAATAAGAGCAAAAACTGGAAGAACACCAGCTGTTCGATGCAACTACAACATTTGGAGTGAGGTACAACAATTGCATGAAATAACTCTGTGTTATAATTGGAATGCTACTGCTTTTGTGGACTGCCCTTTGAACGCCAACAAATGCGATAATTACTTTGTGTGGTATCCGTGGAACACTGCCAAGGTGTTGTCTAAAGAATGA

Coding sequence (CDS)

ATGGCGAAGAAAGAACTTGTATTGATTTATAGTATGTTAGTACTGATAGCAAGTTGCGAGGGACTCAATGAGTATGACTACTTGCAAGTTGTGATTCAATGGCAACCAGCCACGTGTAGTGATTGGAACAAACCATATCCTTATTACTTTCAAGCTCCAGATAATCGATTCAGTATCCATGGCATGTGGCCCAGTCTATACAATGGTGGAGAAACCCAATGTTCTGGCGAGCCATTCGATGTAAACAAGATTAGCACACTACAACCTGGAATTAGTGAAATCTGGCCAAATGTTGCCACAGGAAACAACCTATGGCTATGGGGTCACGAGTGGGATAAGCATGGAACCTGCACAGAAGCCATTTTATTTGACCAAGAAGGATATTTCAGTTTTGCAATGGACAAATATATTGAAAACAATTTGTTGTTTATTCTTGGCAATAGCCAAATTACACCTAACGGACAAGTATACTATTTAAGAAATCAGCTTTACGCGGCAATAAGAGCAAAAACTGGAAGAACACCAGCTGTTCGATGCAACTACAACATTTGGAGTGAGGTACAACAATTGCATGAAATAACTCTGTGTTATAATTGGAATGCTACTGCTTTTGTGGACTGCCCTTTGAACGCCAACAAATGCGATAATTACTTTGTGTGGTATCCGTGGAACACTGCCAAGGTGTTGTCTAAAGAATGA

Protein sequence

MAKKELVLIYSMLVLIASCEGLNEYDYLQVVIQWQPATCSDWNKPYPYYFQAPDNRFSIHGMWPSLYNGGETQCSGEPFDVNKISTLQPGISEIWPNVATGNNLWLWGHEWDKHGTCTEAILFDQEGYFSFAMDKYIENNLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAVRCNYNIWSEVQQLHEITLCYNWNATAFVDCPLNANKCDNYFVWYPWNTAKVLSKE

Homology

[Close]

BLAST of Lag0028102 vs. NCBI nr
Match: XP_038903744.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 412.9 bits (1060), Expect = 1.8e-111
Identity = 186/231 (80.52%), Postives = 201/231 (87.01%), Query Frame = 0


Query: 1   MAKKELVLIYSMLVLIAS-CEGLNEYDYLQVVIQWQPATCSDWNKPYPYYFQAPDNRFSI 60
           M KKE+ LI SMLV+I S CEG+N+YDYLQVVIQWQPATC+DW KPYPY FQ PDNRFSI
Sbjct: 1   MEKKEIGLILSMLVMIVSCCEGVNDYDYLQVVIQWQPATCTDWKKPYPYCFQDPDNRFSI 60

Query: 61  HGMWPSLYNGGETQCSGEPFDVNKISTLQPGISEIWPNVATGNNLWLWGHEWDKHGTCTE 120
           HGMWPSLY+G +T C G PFDVN+IS LQPGISEIWPNV TG N WLWGHEWDKHGTCTE
Sbjct: 61  HGMWPSLYSGAQTHCFGSPFDVNQISILQPGISEIWPNVVTGKNQWLWGHEWDKHGTCTE 120

Query: 121 AILFDQEGYFSFAMDKYIENNLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAVRC 180
           +ILFDQE YFSF MDKYIENNL FILG SQITPNGQ+ Y R Q+YAAI AKTG+TPAVRC
Sbjct: 121 SILFDQEEYFSFTMDKYIENNLSFILGKSQITPNGQL-YSRAQIYAAISAKTGKTPAVRC 180

Query: 181 NYNIWSEVQQLHEITLCYNWNATAFVDCPLNANKCDNYFVWYPWNTAKVLS 231
           NYN WS V+QLHEITLCYNWNATAF DCPLNANKCDN F+WYPW+T K LS
Sbjct: 181 NYNYWSGVEQLHEITLCYNWNATAFEDCPLNANKCDNTFMWYPWSTTKALS 230

[Close]

BLAST of Lag0028102 vs. NCBI nr
Match: AEM42996.1 (S-RNase [Siraitia grosvenorii])

HSP 1 Score: 395.2 bits (1014), Expect = 3.9e-106
Identity = 175/231 (75.76%), Postives = 202/231 (87.45%), Query Frame = 0


Query: 1   MAKKELVLIYSMLVLIASCEGLNEYDYLQVVIQWQPATCSDWNKPYPYYFQAPDNRFSIH 60
           MAK+E+VL+ SMLVLIASCEGLNE+DYLQVV+QWQPATCS  NKPYP  +Q PDNR+SIH
Sbjct: 1   MAKREIVLVLSMLVLIASCEGLNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYSIH 60

Query: 61  GMWPSLYNGGETQCSGEPFDVNKISTLQPGISEIWPNVATGNNLWLWGHEWDKHGTCTEA 120
           G+WPSLY+G  TQCSG PF+ N IS LQPGISEIWPN+  GNNLWLWGHEWDKHGTCTE 
Sbjct: 61  GVWPSLYSGAATQCSGSPFNANAISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCTEE 120

Query: 121 ILFDQEGYFSFAMDKYIENNLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAVRCN 180
           ILFDQ+ YFSFAMD YI+ NLL +LGNSQITPNGQ+ Y R+Q+YAAIRAKTG+TPAVRCN
Sbjct: 121 ILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQI-YSRDQVYAAIRAKTGKTPAVRCN 180

Query: 181 YNIWSEVQQLHEITLCYNWNATAFVDCPLNANKCDNYFVWYPWNTAKVLSK 232
           YN W+  QQ+HE++LCYN +A+A VDCPLNANKCDNYFVWY W+T K +S+
Sbjct: 181 YNRWTGEQQMHEVSLCYNHDASAVVDCPLNANKCDNYFVWYSWSTQKAVSE 230

[Close]

BLAST of Lag0028102 vs. NCBI nr
Match: XP_022149793.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 349.7 bits (896), Expect = 1.9e-92
Identity = 162/233 (69.53%), Postives = 189/233 (81.12%), Query Frame = 0


Query: 1   MAKKE-LVLIYSMLVLIASCEGLNEYDYLQVVIQWQPATCSDWNKPYPYYFQAPDNRFSI 60
           MA++E +VL+ SMLVL+A CEGLNE+DYLQVVIQWQPATCSD+NK  P  FQAP NRFSI
Sbjct: 1   MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNK--PACFQAPGNRFSI 60

Query: 61  HGMWPSLYNGGETQCSGEPFDVNKISTLQPGISEIWPNVATGNNLWLWGHEWDKHGTCTE 120
           HG+WPSLY+G  TQCSG PFD N+IS LQPGISEIWPNVATG+N WLW HEWDKHGTC+E
Sbjct: 61  HGVWPSLYDGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDNFWLWQHEWDKHGTCSE 120

Query: 121 AILFDQEGYFSFAMDKYIENNLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAVRC 180
           +  +DQ+ YF F MD YI NNL  IL N+QITPNG+  Y R+Q+YAAIRAKTG+TPAVRC
Sbjct: 121 STFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRT-YTRDQVYAAIRAKTGKTPAVRC 180

Query: 181 NYNIWSEVQQLHEITLCYNWNATAFVDCPLNANKCDNYFVWYPWNTAKVLSKE 233
           NYN  +   QLHEI+LC+  +A+ F DCPLNANKC   FVW  WNT K +S+E
Sbjct: 181 NYNSRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAISEE 230

[Close]

BLAST of Lag0028102 vs. NCBI nr
Match: XP_022158939.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 214.2 bits (544), Expect = 1.2e-51
Identity = 105/233 (45.06%), Postives = 145/233 (62.23%), Query Frame = 0


Query: 1   MAKKELVLIYSMLVLIASCEGLNEYDYLQVVIQWQPATCSDWNKPYPYYFQAPDNRFSIH 60
           M K+E++L+ SM + I+S EG+NE+DY QVV+QWQPATCS          Q P++R+S+H
Sbjct: 1   MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLK-TVCVQPPEDRYSVH 60

Query: 61  GMWPSLYNGGETQCSG-EPFDVNKISTLQPGISEIWPNVATGNNLWLWGHEWDKHGTCTE 120
           G+WPSL +G    CSG  P+D   I T+Q  + + WPNV TG N   W +EW KHGTC+ 
Sbjct: 61  GVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSS 120

Query: 121 AILFDQEGYFSFAMDKYIENNLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAVRC 180
           A L +Q  YF+F +  Y   +L  IL    I PNGQ  Y +  +  AI + TG+ PA+RC
Sbjct: 121 AYL-NQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQ-QYTKTVVQNAIYSATGKWPALRC 180

Query: 181 NYNIWSEVQQLHEITLCYNWNATAFVDCPLNANKCDNYFVWYPWNTAKVLSKE 233
           N N  +   QLHEI+LC++ N    +DCP N   C+ YFVW  W+ +  ++KE
Sbjct: 181 NNNYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE 230

[Close]

BLAST of Lag0028102 vs. NCBI nr
Match: XP_038904516.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 206.1 bits (523), Expect = 3.3e-49
Identity = 101/239 (42.26%), Postives = 128/239 (53.56%), Query Frame = 0


Query: 19  CEGLNEYDYLQVVIQWQPATCSDWNKPYPYYFQAPDNRFSIHGMWPSLYNGGETQCSGEP 78
           CEG+NEYD+L+VV QWQPATC    KPYPY +Q P NRF +       Y   +       
Sbjct: 2   CEGVNEYDFLRVVFQWQPATCGVATKPYPYCYQTPQNRFGLD------YTMAKPNVQASS 61

Query: 79  FDVN----------------------------------KISTLQPGISEIWPNVATGNNL 138
             VN                                  K+  L+P ++E+W NV TG+N 
Sbjct: 62  SQVNLLFYPKAHFVQTSKQLTRARAHITLVLFKGRENYKVKVLEPRLNEMWGNVITGDNQ 121

Query: 139 WLWGHEWDKHGTCTEAILFDQEGYFSFAMDKYIENNLLFILGNSQITPNGQVYYLRNQLY 198
           WLW  EWDKHGTC E ILFDQ GYF + M+     N   IL N  I P  + YY  +++ 
Sbjct: 122 WLWKPEWDKHGTCIEDILFDQSGYFEYTMNTLYYFNPEEILKNGGIWPTQEGYYTLDEIK 181

Query: 199 AAIRAKTGRTPAVRCNYNIWSEVQQLHEITLCYNWNATAFVDCPLNANKCDNY-FVWYP 223
           + I+A+TG+ PAV CNYN W+  QQLHEI+ C       FVDCP NANKC+ +  +WYP
Sbjct: 182 SVIKARTGKLPAVGCNYNHWTSYQQLHEISYCVTKEGQTFVDCPSNANKCNGHSAMWYP 234

[Close]

BLAST of Lag0028102 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 6.4e-27
Identity = 64/187 (34.22%), Postives = 95/187 (50.80%), Query Frame = 0


Query: 25  YDYLQVVIQWQPATCSDWNKPYPYYFQAPDN---RFSIHGMWPSLYNGGETQC-SGEPFD 84
           +D    V QW PA CS     +      P +    F+IHG+WP       T C  G PFD
Sbjct: 1   FDSFWFVQQWPPAVCS-----FQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFD 60

Query: 85  VNKISTLQPGISEIWPNVATGNNLWLWGHEWDKHGTCTEAILFDQEGYFSFAMDKYIENN 144
           + KIS LQ  ++ +WPNV   NN   W HEW KHGTC+E+  F+Q  YF  A+D     +
Sbjct: 61  ITKISHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSES-TFNQAAYFKLAVDMRNNYD 120

Query: 145 LLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAVRCNYNIWSEVQQLHEITLCYNWN 204
           ++  L      PNG+    R  +   ++AK G+ P +RC  +  ++V  L ++  C+  +
Sbjct: 121 IIGALRPHAAGPNGRT-KSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQD 180

Query: 205 ATAFVDC 208
            +  +DC
Sbjct: 181 GSTLIDC 180

[Close]

BLAST of Lag0028102 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 121.7 bits (304), Expect = 1.1e-26
Identity = 67/217 (30.88%), Postives = 115/217 (53.00%), Query Frame = 0


Query: 1   MAKKELVLIYSMLVLIASC----EGLNEYDYLQVVIQWQPATCSDWNKPYPYYFQAPDNR 60
           MA      ++ +L++I  C        ++D+   V QW  + C             P   
Sbjct: 1   MASNSAFSLFLILLIITQCLSVLNAAKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAAD 60

Query: 61  FSIHGMWPSLYNGG-ETQCS-GEPFDVNKISTLQPGISEIWPNVA--TGNNLWLWGHEWD 120
           F IHG+WP+  +G   + C    P+D ++IS L   + + WP +A  +G+    W HEW+
Sbjct: 61  FGIHGLWPNNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWE 120

Query: 121 KHGTCTEAILFDQEGYFSFAMDKYIENNLLFILGNSQITPNGQVYYLRNQLYAAIRAKTG 180
           KHGTC E++L +Q  YF  A+D   + +LL IL  + I P+G+ Y L N +  AI++  G
Sbjct: 121 KHGTCAESVLTNQHAYFKKALDLKNQIDLLSILQGADIHPDGESYDLVN-IRNAIKSAIG 180

Query: 181 RTPAVRCNYNIWSEVQQLHEITLCYNWNATAFVDCPL 210
            TP ++CN +  S   QL+++ +C + + ++ ++CP+
Sbjct: 181 YTPWIQCNVD-QSGNSQLYQVYICVDGSGSSLIECPI 215

[Close]

BLAST of Lag0028102 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 2.4e-26
Identity = 75/219 (34.25%), Postives = 112/219 (51.14%), Query Frame = 0


Query: 3   KKELVLIYSMLVLIASCEGLNEYDYLQVVIQWQPATCSDWNKPYPYYFQA-PDNRFSIHG 62
           KK L+ I  +  L+  C    ++D+   V QW PA+  D  +   Y     PD  FSIHG
Sbjct: 6   KKLLIKIIVVQCLLVLCVTSQDFDFFYFVQQW-PASYCDTRRSCCYPTTGKPDEDFSIHG 65

Query: 63  MWPSLYNGGETQ-CSGE-PFDVNKISTLQPGISEIWPNVA--TGNNLWLWGHEWDKHGTC 122
           +WP+  +G   Q C  E   D ++ S L   + + WP++A  + + L  W HEW KHGTC
Sbjct: 66  LWPNYKDGKWPQNCDRESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWLKHGTC 125

Query: 123 TEAILFDQEGYFSFAMDKYIENNLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAV 182
           +     +Q  YF  A+D   ++NLL  L N+ I P    YY    +  AI    G TP +
Sbjct: 126 S---ALNQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIEKGVGHTPFI 185

Query: 183 RCNYNIWSEVQQLHEITLCYNWNATAFVDCPL--NANKC 215
            CN +      QL+++ LC + +A+ F+DCP+  +  KC
Sbjct: 186 ECNVDSQGN-HQLYQVYLCVDSSASKFIDCPIFPHGGKC 219

[Close]

BLAST of Lag0028102 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.7e-24
Identity = 67/209 (32.06%), Postives = 112/209 (53.59%), Query Frame = 0


Query: 9   IYSMLVLIAS----CEGLNEYDYLQVVIQWQPATCSDWNKPYPYYFQAPDNRFSIHGMWP 68
           + S+LV++ S         ++D+   V QW  + C    K        P   F IHG+WP
Sbjct: 10  LISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWP 69

Query: 69  SLYNGG-ETQC-SGEPFDVNKISTLQPGISEIWPNVA--TGNNLWLWGHEWDKHGTCTEA 128
           +  +G   + C + +PFD + IS L   + + WP +A  +G+    W HEW+KHGTC+E+
Sbjct: 70  NYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSES 129

Query: 129 ILFDQEGYFSFAMDKYIENNLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAVRCN 188
           ++ DQ  YF  A++   + NLL  L  + I P+G+ Y L + +  +I+   G TP V CN
Sbjct: 130 VI-DQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLES-IRDSIKESIGFTPWVECN 189

Query: 189 YNIWSEVQQLHEITLCYNWNATAFVDCPL 210
            +  S   QL+++ LC + + +  ++CP+
Sbjct: 190 RD-GSGNSQLYQVYLCVDRSGSGLIECPV 215

[Close]

BLAST of Lag0028102 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.3e-24
Identity = 64/198 (32.32%), Postives = 105/198 (53.03%), Query Frame = 0


Query: 24  EYDYLQVVIQWQPATCSDWNKPYPYYFQAPDNRFSIHGMWPSLYNGGETQ-CSGEP-FDV 83
           ++D+   V+QW  A C   +         P   F IHG+WP+   GG  Q C+ +  FD 
Sbjct: 21  DFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRFDD 80

Query: 84  NKISTLQPGISEIWPNVATGNN--LWLWGHEWDKHGTCTEAILFDQEGYFSFAMDKYIEN 143
            ++S L   +   WP ++  +N  +  W HEW+KHGTC E+ L DQ  YF   +    + 
Sbjct: 81  LRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESEL-DQHDYFEAGLKLKQKA 140

Query: 144 NLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAVRCNYNIWSEVQQLHEITLCYNW 203
           NLL  L N+ I P+ + Y +++ +   I+   G  P + CN +  S   QL++I LC + 
Sbjct: 141 NLLHALTNAGIKPDDKFYEMKD-IENTIKQVVGFAPGIECNKDS-SHNSQLYQIYLCVDT 200

Query: 204 NATAFVDCPLNAN-KCDN 217
           +A+ F++CP+  + +CD+
Sbjct: 201 SASKFINCPVMPHGRCDS 215

[Close]

BLAST of Lag0028102 vs. ExPASy TrEMBL
Match: K7NBR4 (S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 1.9e-106
Identity = 175/231 (75.76%), Postives = 202/231 (87.45%), Query Frame = 0


Query: 1   MAKKELVLIYSMLVLIASCEGLNEYDYLQVVIQWQPATCSDWNKPYPYYFQAPDNRFSIH 60
           MAK+E+VL+ SMLVLIASCEGLNE+DYLQVV+QWQPATCS  NKPYP  +Q PDNR+SIH
Sbjct: 1   MAKREIVLVLSMLVLIASCEGLNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYSIH 60

Query: 61  GMWPSLYNGGETQCSGEPFDVNKISTLQPGISEIWPNVATGNNLWLWGHEWDKHGTCTEA 120
           G+WPSLY+G  TQCSG PF+ N IS LQPGISEIWPN+  GNNLWLWGHEWDKHGTCTE 
Sbjct: 61  GVWPSLYSGAATQCSGSPFNANAISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCTEE 120

Query: 121 ILFDQEGYFSFAMDKYIENNLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAVRCN 180
           ILFDQ+ YFSFAMD YI+ NLL +LGNSQITPNGQ+ Y R+Q+YAAIRAKTG+TPAVRCN
Sbjct: 121 ILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQI-YSRDQVYAAIRAKTGKTPAVRCN 180

Query: 181 YNIWSEVQQLHEITLCYNWNATAFVDCPLNANKCDNYFVWYPWNTAKVLSK 232
           YN W+  QQ+HE++LCYN +A+A VDCPLNANKCDNYFVWY W+T K +S+
Sbjct: 181 YNRWTGEQQMHEVSLCYNHDASAVVDCPLNANKCDNYFVWYSWSTQKAVSE 230

[Close]

BLAST of Lag0028102 vs. ExPASy TrEMBL
Match: A0A6J1D7Q5 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 9.1e-93
Identity = 162/233 (69.53%), Postives = 189/233 (81.12%), Query Frame = 0


Query: 1   MAKKE-LVLIYSMLVLIASCEGLNEYDYLQVVIQWQPATCSDWNKPYPYYFQAPDNRFSI 60
           MA++E +VL+ SMLVL+A CEGLNE+DYLQVVIQWQPATCSD+NK  P  FQAP NRFSI
Sbjct: 1   MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNK--PACFQAPGNRFSI 60

Query: 61  HGMWPSLYNGGETQCSGEPFDVNKISTLQPGISEIWPNVATGNNLWLWGHEWDKHGTCTE 120
           HG+WPSLY+G  TQCSG PFD N+IS LQPGISEIWPNVATG+N WLW HEWDKHGTC+E
Sbjct: 61  HGVWPSLYDGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDNFWLWQHEWDKHGTCSE 120

Query: 121 AILFDQEGYFSFAMDKYIENNLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAVRC 180
           +  +DQ+ YF F MD YI NNL  IL N+QITPNG+  Y R+Q+YAAIRAKTG+TPAVRC
Sbjct: 121 STFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRT-YTRDQVYAAIRAKTGKTPAVRC 180

Query: 181 NYNIWSEVQQLHEITLCYNWNATAFVDCPLNANKCDNYFVWYPWNTAKVLSKE 233
           NYN  +   QLHEI+LC+  +A+ F DCPLNANKC   FVW  WNT K +S+E
Sbjct: 181 NYNSRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAISEE 230

[Close]

BLAST of Lag0028102 vs. ExPASy TrEMBL
Match: A0A6J1E0X1 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 5.9e-52
Identity = 105/233 (45.06%), Postives = 145/233 (62.23%), Query Frame = 0


Query: 1   MAKKELVLIYSMLVLIASCEGLNEYDYLQVVIQWQPATCSDWNKPYPYYFQAPDNRFSIH 60
           M K+E++L+ SM + I+S EG+NE+DY QVV+QWQPATCS          Q P++R+S+H
Sbjct: 1   MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLK-TVCVQPPEDRYSVH 60

Query: 61  GMWPSLYNGGETQCSG-EPFDVNKISTLQPGISEIWPNVATGNNLWLWGHEWDKHGTCTE 120
           G+WPSL +G    CSG  P+D   I T+Q  + + WPNV TG N   W +EW KHGTC+ 
Sbjct: 61  GVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSS 120

Query: 121 AILFDQEGYFSFAMDKYIENNLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAVRC 180
           A L +Q  YF+F +  Y   +L  IL    I PNGQ  Y +  +  AI + TG+ PA+RC
Sbjct: 121 AYL-NQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQ-QYTKTVVQNAIYSATGKWPALRC 180

Query: 181 NYNIWSEVQQLHEITLCYNWNATAFVDCPLNANKCDNYFVWYPWNTAKVLSKE 233
           N N  +   QLHEI+LC++ N    +DCP N   C+ YFVW  W+ +  ++KE
Sbjct: 181 NNNYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE 230

[Close]

BLAST of Lag0028102 vs. ExPASy TrEMBL
Match: A0A6J1E266 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 1.1e-48
Identity = 102/224 (45.54%), Postives = 137/224 (61.16%), Query Frame = 0


Query: 1   MAKKELVLIYSMLVLIASCEGLNEYDYLQVVIQWQPATCSDWNKPYPYYFQAPDNRFSIH 60
           M KK +VL++S LV+IASC    +Y+Y QV +QWQPATCSD N   P     P +RFSIH
Sbjct: 1   MEKKGIVLVFSTLVVIASC----DYNYYQVTLQWQPATCSDANLQTP-CVNTPQDRFSIH 60

Query: 61  GMWPSLYNGGETQCSGEP-FDVNKISTLQPGISEIWPNVATGNNLWLWGHEWDKHGTCTE 120
           G+WP+L +G   QC G P +D +KIST++  ++  WP+V  G N  LW HEW  HGTC+ 
Sbjct: 61  GVWPTLSSGSYPQCPGTPAYDGSKISTIRNRLNADWPSVIYGTNPTLWSHEWTTHGTCSY 120

Query: 121 AILFDQEGYFSFAMDKYIENNLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAVRC 180
             L  Q  YF+F +D Y + ++   L    I P+  V Y ++ +  AIR  T + P +RC
Sbjct: 121 PSL-SQLDYFTFGLDTYAKYDIYSALDKEDIYPSEDV-YSKSSINNAIRKITQKLPGLRC 180

Query: 181 NYNIWSEVQQLHEITLCYNWNATAFVDCPLNANKCDNYFVWYPW 224
           N N +S  +QLHEI LC+  +  A +DCP N   C NY  WYPW
Sbjct: 181 NTNDYSRERQLHEIILCFAKSNRAIIDCPSNVVTCGNYVYWYPW 217

[Close]

BLAST of Lag0028102 vs. ExPASy TrEMBL
Match: A0A6J1DQK2 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 2.6e-47
Identity = 107/231 (46.32%), Postives = 145/231 (62.77%), Query Frame = 0


Query: 1   MAKK-ELVLIYSMLVLIASCEGLNEYDYLQVVIQWQPATCSDWNKPYPYYFQAPDNRFSI 60
           MAK+ E+VLI SML+LI   EG+NE+DY QVV+QWQPATCS  +       Q PD RFSI
Sbjct: 1   MAKRSEIVLILSMLLLIGWSEGVNEFDYYQVVLQWQPATCSS-STLVDECVQPPDERFSI 60

Query: 61  HGMWPSLYNGGETQCSG-EPFDVNKI-STLQPGISEIWPNVATGNNLWLWGHEWDKHGTC 120
           HG+WPSL + G+  CSG  P+D  ++ S+++  + + WP+V    +   W HEW  HGTC
Sbjct: 61  HGVWPSLLS-GDAVCSGTPPYDGTQLNSSIRARLHQDWPSVVHRVDPPFWSHEWTLHGTC 120

Query: 121 TEAILFDQEGYFSFAMDKYIENNLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAV 180
           + A L DQ  YF F +  Y   ++L IL    + PNG + Y R+ +   I + TG+ PA+
Sbjct: 121 SYAYL-DQFHYFDFGLFVYGNYDVLGILNYYNVYPNGNL-YARSDVENIIYSATGKWPAL 180

Query: 181 RCNYNIWSEVQQLHEITLCYNWNATAFVDCPLNANKCDNYFVWYPWNTAKV 229
           RCN N WS   QLHEI+LC+     +  DCP NA KC++YFVW  W++  V
Sbjct: 181 RCNNNFWSGDVQLHEISLCFRKQDFSLEDCPYNAVKCNDYFVWNSWSSNAV 227

[Close]

BLAST of Lag0028102 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 120.9 bits (302), Expect = 1.3e-27
Identity = 70/192 (36.46%), Postives = 108/192 (56.25%), Query Frame = 0


Query: 24  EYDYLQVVIQWQPATCSDWNKPYPYYFQA-PDNRFSIHGMWPSLYNGGETQCSGEP---F 83
           ++D+   V+QW  A C D ++   Y     P   F IHG+WP+ YNGG    + +P   F
Sbjct: 27  DFDFFYFVLQWPGAYC-DTSRACCYPTSGKPAADFGIHGLWPN-YNGGSWPSNCDPDSQF 86

Query: 84  DVNKISTLQPGISEIWPNVATGNN--LWLWGHEWDKHGTCTEAILFDQEGYFSFAMDKYI 143
           D ++IS L   + + WP ++  +N     W HEW+KHGTC+E+++ DQ  YF  A+    
Sbjct: 87  DRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVM-DQHEYFENALKLKQ 146

Query: 144 ENNLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAVRCNYNIWSEVQQLHEITLCY 203
           + NLL IL NS I P+   Y L +++  AI+   G TP + CN +      QLH+I +C 
Sbjct: 147 KANLLQILKNSGINPDDGFYNL-DKITNAIKDGIGFTPGIECNKDP-ERNAQLHQIYICV 206

Query: 204 NWNATAFVDCPL 210
           + + T F++CP+
Sbjct: 207 DTSGTEFIECPV 213

[Close]

BLAST of Lag0028102 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 114.4 bits (285), Expect = 1.2e-25
Identity = 67/209 (32.06%), Postives = 112/209 (53.59%), Query Frame = 0


Query: 9   IYSMLVLIAS----CEGLNEYDYLQVVIQWQPATCSDWNKPYPYYFQAPDNRFSIHGMWP 68
           + S+LV++ S         ++D+   V QW  + C    K        P   F IHG+WP
Sbjct: 10  LISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWP 69

Query: 69  SLYNGG-ETQC-SGEPFDVNKISTLQPGISEIWPNVA--TGNNLWLWGHEWDKHGTCTEA 128
           +  +G   + C + +PFD + IS L   + + WP +A  +G+    W HEW+KHGTC+E+
Sbjct: 70  NYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSES 129

Query: 129 ILFDQEGYFSFAMDKYIENNLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAVRCN 188
           ++ DQ  YF  A++   + NLL  L  + I P+G+ Y L + +  +I+   G TP V CN
Sbjct: 130 VI-DQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLES-IRDSIKESIGFTPWVECN 189

Query: 189 YNIWSEVQQLHEITLCYNWNATAFVDCPL 210
            +  S   QL+++ LC + + +  ++CP+
Sbjct: 190 RD-GSGNSQLYQVYLCVDRSGSGLIECPV 215

[Close]

BLAST of Lag0028102 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 114.0 bits (284), Expect = 1.6e-25
Identity = 64/198 (32.32%), Postives = 105/198 (53.03%), Query Frame = 0


Query: 24  EYDYLQVVIQWQPATCSDWNKPYPYYFQAPDNRFSIHGMWPSLYNGGETQ-CSGEP-FDV 83
           ++D+   V+QW  A C   +         P   F IHG+WP+   GG  Q C+ +  FD 
Sbjct: 21  DFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRFDD 80

Query: 84  NKISTLQPGISEIWPNVATGNN--LWLWGHEWDKHGTCTEAILFDQEGYFSFAMDKYIEN 143
            ++S L   +   WP ++  +N  +  W HEW+KHGTC E+ L DQ  YF   +    + 
Sbjct: 81  LRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESEL-DQHDYFEAGLKLKQKA 140

Query: 144 NLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAVRCNYNIWSEVQQLHEITLCYNW 203
           NLL  L N+ I P+ + Y +++ +   I+   G  P + CN +  S   QL++I LC + 
Sbjct: 141 NLLHALTNAGIKPDDKFYEMKD-IENTIKQVVGFAPGIECNKDS-SHNSQLYQIYLCVDT 200

Query: 204 NATAFVDCPLNAN-KCDN 217
           +A+ F++CP+  + +CD+
Sbjct: 201 SASKFINCPVMPHGRCDS 215

[Close]

BLAST of Lag0028102 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 100.1 bits (248), Expect = 2.4e-21
Identity = 67/217 (30.88%), Postives = 104/217 (47.93%), Query Frame = 0


Query: 7   VLIYSMLVL----IASCEGLNEYDYLQVVIQWQPATCSDWNKPYPYYFQAPDNRFSIHGM 66
           ++I S+L+L    ++S +   ++++   V  W  A C       P       + F IHG+
Sbjct: 4   IIIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFIIHGL 63

Query: 67  WPSLYNGGETQCSGEP--FDVNKISTLQPGISEIWPN----VATGNNLWLWGHEWDKHGT 126
           WP   NG       +   FD++KIS L   + + W          N   LW HEW+KHGT
Sbjct: 64  WPQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKHGT 123

Query: 127 CTEAILFDQEGYFSFAMDKYIENNLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPA 186
           C ++I FDQ  YF   +    + +LL IL    I PN   Y L +++  AI+   G  P 
Sbjct: 124 CVQSI-FDQHSYFRTNLKFKHKVHLLNILIQKGIKPNDGFYSL-DEIKNAIKCAIGFAPG 183

Query: 187 VRCNYNIWSEVQQLHEITLCYNWNATAFVDCPLNANK 214
           + CN ++    +QL +I +C +  A  FV+CP   +K
Sbjct: 184 IECNEDVKGN-KQLFQIYICLDNYAKEFVECPYVPDK 217

[Close]

BLAST of Lag0028102 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 90.1 bits (222), Expect = 2.5e-18
Identity = 50/178 (28.09%), Postives = 82/178 (46.07%), Query Frame = 0


Query: 24  EYDYLQVVIQWQPATCSD----WNKPYPYYFQAPDNRFSIHGMWPSLYNGGE--TQCSGE 83
           E+DY  + +QW    C       +K           +F+IHG+WP  YN G   + C   
Sbjct: 31  EFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPD-YNDGSWPSCCYRS 90

Query: 84  PFDVNKISTLQPGISEIWPNVA-------TGNNLWLWGHEWDKHGTCTEAILFDQEGYFS 143
            F   +ISTL  G+ + WP+++        G     WGHEW+KHGTC+  +  D+  YF 
Sbjct: 91  DFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFL 150

Query: 144 FAMDKYIENNLLFILGNSQITPNGQVYYLRNQLYAAIRAKTGRTPAVRCNYNIWSEVQ 189
             ++ Y+++N+  +L  +    +    Y    +  AI+     TP V C  +   E++
Sbjct: 151 TTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRDAIDEIR 207

The following BLAST results are available for this feature:

BLAST of Lag0028102 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903744.1	1.8e-111	80.52	ribonuclease MC-like [Benincasa hispida]	[more]
AEM42996.1	3.9e-106	75.76	S-RNase [Siraitia grosvenorii]	[more]
XP_022149793.1	1.9e-92	69.53	ribonuclease MC-like [Momordica charantia]	[more]
XP_022158939.1	1.2e-51	45.06	ribonuclease MC-like [Momordica charantia]	[more]
XP_038904516.1	3.3e-49	42.26	ribonuclease MC-like [Benincasa hispida]	[more]

BLAST of Lag0028102 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	6.4e-27	34.22	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P80022	1.1e-26	30.88	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P80196	2.4e-26	34.25	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]
P42813	1.7e-24	32.06	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P42815	2.3e-24	32.32	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]

BLAST of Lag0028102 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
K7NBR4	1.9e-106	75.76	S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1	[more]
A0A6J1D7Q5	9.1e-93	69.53	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1	[more]
A0A6J1E0X1	5.9e-52	45.06	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1	[more]
A0A6J1E266	1.1e-48	45.54	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1	[more]
A0A6J1DQK2	2.6e-47	46.32	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1	[more]

BLAST of Lag0028102 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14220.1	1.3e-27	36.46	Ribonuclease T2 family protein	[more]
AT2G02990.1	1.2e-25	32.06	ribonuclease 1	[more]
AT1G26820.1	1.6e-25	32.32	ribonuclease 3	[more]
AT1G14210.1	2.4e-21	30.88	Ribonuclease T2 family protein	[more]
AT2G39780.1	2.5e-18	28.09	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 25..207 e-value: 1.9E-37 score: 129.2
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 10..218
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 20..230 e-value: 9.9E-49 score: 168.2
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 25..219
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 10..218
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..19 score: 6.0
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 106..117
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 25..208 e-value: 5.49005E-53 score: 167.509

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0028102.1	Lag0028102.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding