Lag0028069 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0028069 vs. NCBI nr
Match: XP_004146405.3 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KAE8649721.1 hypothetical protein Csa_011975 [Cucumis sativus])

HSP 1 Score: 772.7 bits (1994), Expect = 2.2e-219
Identity = 415/567 (73.19%), Postives = 465/567 (82.01%), Query Frame = 0

Query: 1   MAAAVGFCLKLLTGRWLILFASLTIMAMNGSGYMFGLYSNHIKSFFGYDQSALNLVSFFK 60
           +AA  GF  KLLTGRWL+LFASL IMAMNGSGYMFGLYSNHIKS FGYDQS LNL+SFFK
Sbjct: 6   LAAVTGFLHKLLTGRWLMLFASLIIMAMNGSGYMFGLYSNHIKSVFGYDQSTLNLISFFK 65

Query: 61  DLGANLGVLSGLLYEVAPPWLVLSVGAALNFIAYFMVWAAISGRTAAPGLWLMCLYMGVA 120
           DLGANLGV+SGLLYEVAPPWLVLSVGA LNF  YFM+W A+SGRTAAPGL LMC+YM VA
Sbjct: 66  DLGANLGVVSGLLYEVAPPWLVLSVGAILNFFGYFMLWLAVSGRTAAPGLRLMCVYMSVA 125

Query: 121 ANSLSFSNTAALVTCVRNFPQYRGCLLGLLKGYIGLSGAIMTQLYHAMYGDDS-KSLILL 180
           ANSLSF NTAALVTC+RNFP +RGCLLGLLKGYIGLSGAIMTQLYHAMYG+D+ + LIL+
Sbjct: 126 ANSLSFGNTAALVTCLRNFPLHRGCLLGLLKGYIGLSGAIMTQLYHAMYGEDNPEGLILM 185

Query: 181 IAWLPAVVSLASLPVIRLINANNQQPNDDLKAFYALLYISLGLAASLIAIIIAQTKLQFS 240
           IAWLP+ +SLASLP IRLIN+NN Q N DLK FY LLYISL LAASL+AIII QTK  FS
Sbjct: 186 IAWLPSAISLASLPFIRLINSNNNQRN-DLKPFYNLLYISLALAASLLAIIIPQTKTHFS 245

Query: 241 KAEYIAAASPVILFLLLPLAVVVNQELKLPKTTVDNPPQSTVLVEPP---------QMSW 300
           K +YIA ASP++LFLLLPLAVVVNQEL L       PP +++LV+ P           +W
Sbjct: 246 KTDYIAVASPIVLFLLLPLAVVVNQELTLHN---HPPPITSILVQSPSPQLTTMSRSSNW 305

Query: 301 YKNIF-NRPPPGEDHTILQAILNVDMLILFIATTCGVGGALTVVDNLAQIGASLHYPPRS 360
           YKNIF  RP  G+DHTILQAIL+VDM ILF+ TTCGVGGALTVVDN+AQIGASL YP RS
Sbjct: 306 YKNIFTGRPMLGDDHTILQAILSVDMAILFVVTTCGVGGALTVVDNVAQIGASLDYPTRS 365

Query: 361 ISTFVSLLSIWNFLGRVVAGYASEFLLINYRLPRPLMLTAVILLSSFGHVLIAFAVPNSL 420
           IS+FVSL+SIWNFLGRV+AGY SEFLLI YRLPRPLMLT VILLS  GH++IAF VPNSL
Sbjct: 366 ISSFVSLMSIWNFLGRVMAGYVSEFLLIKYRLPRPLMLTFVILLSCIGHIMIAFGVPNSL 425

Query: 421 YLASMITGFSLGAQLPLTAIIISELFGLKHYSTLYNVGSVSSPVGSYIFNVRVAGRLYDR 480
           Y AS+ITGF LGAQLPLTA IIS+LFGLKHYSTLYNVGSVSSPVGSYIFNVR+AGR+YDR
Sbjct: 426 YFASIITGFCLGAQLPLTATIISDLFGLKHYSTLYNVGSVSSPVGSYIFNVRLAGRIYDR 485

Query: 481 EGERQNAEMGLVRKAGQELMCRGATCYRVSFLVMMAASAFGSLVSVVLVLRTRGFYRGDI 540
           EGERQ   M  V        C+G  CYRVSF++++ A  FGSLVSV+LVLRTR FY+ DI
Sbjct: 486 EGERQRNVMRNV--------CKGVRCYRVSFIIIIGACVFGSLVSVILVLRTRNFYKDDI 545

Query: 541 YGRFKKVQGENEEIAGTALSTPTQGLT 557
           Y RF+K +     +  T  +T T  L+
Sbjct: 546 YARFRKGEEIKSPLQDTTTTTTTFSLS 560

BLAST of Lag0028069 vs. NCBI nr
Match: TYK05449.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 705.3 bits (1819), Expect = 4.2e-199
Identity = 377/484 (77.89%), Postives = 415/484 (85.74%), Query Frame = 0

Query: 2   AAAVGFCLKLLTGRWLILFASLTIMAMNGSGYMFGLYSNHIKSFFGYDQSALNLVSFFKD 61
           AAA GF  KLLTGRWL+LFASL IMAMNGSGYMFGLYSNHIKS FGYDQSALN +SFFKD
Sbjct: 7   AAATGFLHKLLTGRWLMLFASLIIMAMNGSGYMFGLYSNHIKSIFGYDQSALNSISFFKD 66

Query: 62  LGANLGVLSGLLYEVAPPWLVLSVGAALNFIAYFMVWAAISGRTAAPGLWLMCLYMGVAA 121
           LGANLGV+SGLLYEVAPPWLVLSVGA LNF  YFM+W A+SGRTAAPGLWLMC+YMGVAA
Sbjct: 67  LGANLGVVSGLLYEVAPPWLVLSVGAILNFFGYFMLWLAVSGRTAAPGLWLMCVYMGVAA 126

Query: 122 NSLSFSNTAALVTCVRNFPQYRGCLLGLLKGYIGLSGAIMTQLYHAMYGDDS-KSLILLI 181
           NSLSF NTAALVTC+RNFP +RGCLLGLLKGYIGLSGAIMTQLYHAMYG+++ + LIL+I
Sbjct: 127 NSLSFGNTAALVTCLRNFPLHRGCLLGLLKGYIGLSGAIMTQLYHAMYGENNPEGLILMI 186

Query: 182 AWLPAVVSLASLPVIRLINANNQQPNDDLKAFYALLYISLGLAASLIAIIIAQTKLQFSK 241
           AWLP+ +SLASLP IR IN+NN Q N DLK FY LLYISL LAASL+AIII QTK+ FSK
Sbjct: 187 AWLPSAISLASLPFIRHINSNNNQRN-DLKPFYNLLYISLALAASLLAIIIPQTKIHFSK 246

Query: 242 AEYIAAASPVILFLLLPLAVVVNQELKLPKTTVDNPPQSTVLVEPP---------QMSWY 301
            +YIA AS VILFLLLPLA+VVNQEL L       PP +++LV+             +WY
Sbjct: 247 TDYIAVASLVILFLLLPLAIVVNQELTLHN---HPPPITSILVQSSSPHLTTMSRSSNWY 306

Query: 302 KNIF-NRPPPGEDHTILQAILNVDMLILFIATTCGVGGALTVVDNLAQIGASLHYPPRSI 361
           KNIF  RP  G+DHTILQAIL+VDM ILF+ATTCGVGG LTVVDN+AQIGASL YP RSI
Sbjct: 307 KNIFTGRPVFGDDHTILQAILSVDMAILFMATTCGVGGVLTVVDNVAQIGASLDYPTRSI 366

Query: 362 STFVSLLSIWNFLGRVVAGYASEFLLINYRLPRPLMLTAVILLSSFGHVLIAFAVPNSLY 421
           S+FVSL+SIWNFLGRV+AGY SEFLL+ YR PRPLMLT VILLS  GH++IAF VPNSLY
Sbjct: 367 SSFVSLMSIWNFLGRVMAGYVSEFLLMKYRFPRPLMLTFVILLSCIGHIMIAFGVPNSLY 426

Query: 422 LASMITGFSLGAQLPLTAIIISELFGLKHYSTLYNVGSVSSPVGSYIFNVRVAGRLYDRE 475
             S+ITGF LGAQLPLTA IIS+LFGLKHYSTLYNVGSVSSPVGSYIFNVRVAGR+YDRE
Sbjct: 427 FVSIITGFCLGAQLPLTATIISDLFGLKHYSTLYNVGSVSSPVGSYIFNVRVAGRVYDRE 486

BLAST of Lag0028069 vs. NCBI nr
Match: POO01771.1 (Major facilitator [Trema orientale])

HSP 1 Score: 636.3 bits (1640), Expect = 2.4e-178
Identity = 340/577 (58.93%), Postives = 426/577 (73.83%), Query Frame = 0

Query: 7   FCLKLLTGRWLILFASLTIMAMNGSGYMFGLYSNHIKSFFGYDQSALNLVSFFKDLGANL 66
           F + +L  +W ++FA+L IM++NGSGYMFGLYSN IKS  GYDQS LNL+SFFKDLG NL
Sbjct: 14  FVVHVLWSKWFMVFATLLIMSVNGSGYMFGLYSNDIKSTLGYDQSTLNLISFFKDLGGNL 73

Query: 67  GVLSGLLYEVAPPWLVLSVGAALNFIAYFMVWAAISGRTAAPGLWLMCLYMGVAANSLSF 126
           GV +GL+ EV+PPWLVLS+GAA+NF  YFM+W +I+GR A P +W MCLY+ + ANSLSF
Sbjct: 74  GVTAGLINEVSPPWLVLSIGAAMNFFGYFMIWTSITGRVAKPHVWQMCLYICIGANSLSF 133

Query: 127 SNTAALVTCVRNFPQYRGCLLGLLKGYIGLSGAIMTQLYHAMYGDDSKSLILLIAWLPAV 186
            NTAALV CV+NFP+ RG ++GLLKG IGLSGAI+TQLYHA+YGDDS++L+LLIAW P V
Sbjct: 134 PNTAALVACVKNFPESRGSVIGLLKGLIGLSGAILTQLYHAIYGDDSEALVLLIAWFPPV 193

Query: 187 VSLASLPVIRLINANNQQPNDDLKAFYALLYISLGLAASLIAIIIAQTKLQFSKAEYIAA 246
           VSL  L  IR++  + +QPN +L+ F   LYISLG+A SL+A+II Q +L F++ +Y A+
Sbjct: 194 VSLIFLRTIRIMK-SVRQPN-ELRVFCNFLYISLGVAGSLMAMIIIQNRLHFNRVQYAAS 253

Query: 247 ASPVILFLLLPLAVVVNQELK-------LPKTTVDNPP---------QSTVLVEP----- 306
           AS +++ L L L VV+N+ELK         K   +NPP         Q+ +  EP     
Sbjct: 254 ASVILVVLFLSLFVVINEELKSWKLKKQALKEPSENPPISAPLPVIAQNPLASEPQPVIN 313

Query: 307 --------PQMSWYKNIFNRPPPGEDHTILQAILNVDMLILFIATTCGVGGALTVVDNLA 366
                   P +SW K IF  P  GED+TILQA L+ DMLILFIATTCGVGG LT++DNL 
Sbjct: 314 RVPPSSLKPYLSWVKTIFKAPDRGEDYTILQAALSTDMLILFIATTCGVGGTLTLIDNLG 373

Query: 367 QIGASLHYPPRSISTFVSLLSIWNFLGRVVAGYASEFLLINYRLPRPLMLTAVILLSSFG 426
           Q+G+SL YP  SI+TF+SL+SIWNFLGRV AG+ SE LL  Y+ PRP MLTAVILLS  G
Sbjct: 374 QLGSSLGYPKHSIATFISLISIWNFLGRVAAGFVSEILLTKYKFPRPSMLTAVILLSCVG 433

Query: 427 HVLIAFAVPNSLYLASMITGFSLGAQLPLTAIIISELFGLKHYSTLYNVGSVSSPVGSYI 486
           H+LIAF+VP+SLY  S+I+GF  GAQL L   IISELFGLK+YSTLYNVG++SSP+GSYI
Sbjct: 434 HLLIAFSVPHSLYFTSIISGFCYGAQLSLLFAIISELFGLKYYSTLYNVGALSSPLGSYI 493

Query: 487 FNVRVAGRLYDREGERQNAEMGLVRKAGQELMCRGATCYRVSFLVMMAASAFGSLVSVVL 546
            NVRVAGRLYD+E  +Q   +GLVRKAGQEL C G  CYR +F+V+ A + FGSLVS +L
Sbjct: 494 LNVRVAGRLYDKEALKQMEALGLVRKAGQELTCNGGKCYRTAFIVITAVTLFGSLVSFIL 553

Query: 547 VLRTRGFYRGDIYGRFKKVQGENEEIAGTALSTPTQG 555
           VLRTR FY+ DIY +F++   E   I  T ++    G
Sbjct: 554 VLRTRKFYKSDIYKKFRE---EERAITETQIAPTGNG 585

BLAST of Lag0028069 vs. NCBI nr
Match: KAB2066363.1 (hypothetical protein ES319_A09G155000v1 [Gossypium barbadense] >TYH02885.1 hypothetical protein ES288_A09G176300v1 [Gossypium darwinii])

HSP 1 Score: 636.0 bits (1639), Expect = 3.2e-178
Identity = 329/534 (61.61%), Postives = 407/534 (76.22%), Query Frame = 0

Query: 7   FCLKLLTGRWLILFASLTIMAMNGSGYMFGLYSNHIKSFFGYDQSALNLVSFFKDLGANL 66
           F   +L+ R  ++FA+L IM+ NGSGYMFGLYSN IKS  GYDQ+ LNL+SFFKDLG NL
Sbjct: 14  FVFDVLSSRLFMVFATLIIMSANGSGYMFGLYSNDIKSTLGYDQTTLNLLSFFKDLGGNL 73

Query: 67  GVLSGLLYEVAPPWLVLSVGAALNFIAYFMVWAAISGRTAAPGLWLMCLYMGVAANSLSF 126
           G+LSGL+YEV PPW+VL +G+ +NF  YFM+W +++GR A P  W MCLYM +AANS SF
Sbjct: 74  GILSGLIYEVVPPWVVLCMGSLMNFFGYFMLWVSVTGRIAKPHAWQMCLYMWIAANSQSF 133

Query: 127 SNTAALVTCVRNFPQYRGCLLGLLKGYIGLSGAIMTQLYHAMYGDDSKSLILLIAWLPAV 186
            NT ALVTCV+NFP+ RG +LGLLKG+IGLSGA+MTQ YHA Y DDSK+LILL+AWLP +
Sbjct: 134 PNTGALVTCVKNFPENRGSVLGLLKGFIGLSGAVMTQYYHAFYTDDSKALILLLAWLPTI 193

Query: 187 VSLASLPVIRLINANNQQPNDDLKAFYALLYISLGLAASLIAIIIAQTKLQFSKAEYIAA 246
           V L  L  IR++         +L+ FY  LYI+LGLA  ++ +I+ Q K++F++ EY+A+
Sbjct: 194 VPLIFLRTIRILKVVRMA--RELEIFYRFLYIALGLAGFIMVLIVLQNKVRFTRTEYVAS 253

Query: 247 ASPVILFLLLPLAVVVNQELKL---PKTTVDNPPQSTVL--VEPPQMSWYKNIFNRPPPG 306
           A  VI+ LLLPL +V+ +EL +    K   +N  Q  V    + P  +  K IF+ P  G
Sbjct: 254 AFVVIVLLLLPLVIVIKEELSVWTGKKQASNNHSQVNVASGSDDPAAACIKTIFDPPERG 313

Query: 307 EDHTILQAILNVDMLILFIATTCGVGGALTVVDNLAQIGASLHYPPRSISTFVSLLSIWN 366
           ED+ ILQAI ++DMLILFIAT CG GGALTV+DNL Q+G SL YP  SISTF+SL+SIWN
Sbjct: 314 EDYGILQAIFSIDMLILFIATACGTGGALTVIDNLGQLGKSLGYPKHSISTFISLVSIWN 373

Query: 367 FLGRVVAGYASEFLLINYRLPRPLMLTAVILLSSFGHVLIAFAVPNSLYLASMITGFSLG 426
           F GRV+AGY SE  L NY  PRPLMLT VIL S  GH+LIAFAVPNSLY AS++TGF LG
Sbjct: 374 FFGRVLAGYLSEMALTNYNFPRPLMLTLVILSSCIGHLLIAFAVPNSLYFASVMTGFCLG 433

Query: 427 AQLPLTAIIISELFGLKHYSTLYNVGSVSSPVGSYIFNVRVAGRLYDREGERQNAEMGLV 486
           AQLPL   IISELFGLKHYST+YNVGSVSSPVGSYIFNVRVAG LYD+E  +Q   +GL 
Sbjct: 434 AQLPLLCAIISELFGLKHYSTMYNVGSVSSPVGSYIFNVRVAGHLYDKEALKQMEALGLE 493

Query: 487 RKAGQELMCRGATCYRVSFLVMMAASAFGSLVSVVLVLRTRGFYRGDIYGRFKK 536
           RKAGQ+L C GATCYR++FL++ AA+  GS+VS++LV RTR FY+GDIY +FK+
Sbjct: 494 RKAGQDLTCNGATCYRLAFLIISAATLLGSIVSLILVFRTRTFYKGDIYKKFKE 545

BLAST of Lag0028069 vs. NCBI nr
Match: MBA0800705.1 (hypothetical protein [Gossypium harknessii])

HSP 1 Score: 636.0 bits (1639), Expect = 3.2e-178
Identity = 329/545 (60.37%), Postives = 410/545 (75.23%), Query Frame = 0

Query: 7   FCLKLLTGRWLILFASLTIMAMNGSGYMFGLYSNHIKSFFGYDQSALNLVSFFKDLGANL 66
           F   +L+ R  ++FA+L IM++NGSGYMFGLYSN IKS  GYDQ+ LNL+SFFKDLG NL
Sbjct: 14  FVFDVLSSRLFMVFATLIIMSVNGSGYMFGLYSNDIKSTLGYDQTTLNLLSFFKDLGGNL 73

Query: 67  GVLSGLLYEVAPPWLVLSVGAALNFIAYFMVWAAISGRTAAPGLWLMCLYMGVAANSLSF 126
           G+LSGL+YEV PPW+VL +G+ +NF  YFM+W +++GR A P  W MCLYM +AANS SF
Sbjct: 74  GILSGLIYEVVPPWVVLCMGSLMNFFGYFMIWVSVTGRIAKPHAWQMCLYMWIAANSQSF 133

Query: 127 SNTAALVTCVRNFPQYRGCLLGLLKGYIGLSGAIMTQLYHAMYGDDSKSLILLIAWLPAV 186
            NT ALVTCV+NFP+ RG +LGLLKG+IGLSGAIMTQ YHA Y DD+K+LILL+AWLP +
Sbjct: 134 PNTGALVTCVKNFPENRGSVLGLLKGFIGLSGAIMTQYYHAFYMDDTKALILLLAWLPTI 193

Query: 187 VSLASLPVIRLINANNQQPNDDLKAFYALLYISLGLAASLIAIIIAQTKLQFSKAEYIAA 246
           V L  L  IR++         +L+ FY  LYI+LGLA  ++ +I+ Q K++F++ EY+A+
Sbjct: 194 VPLIFLRTIRIMKVVRMA--RELEIFYRFLYIALGLAGFIMVLIVLQNKVRFTRTEYVAS 253

Query: 247 ASPVILFLLLPLAVVVNQELKL---PKTTVDNPPQSTVLVEPP-------------QMSW 306
           A  V++ LLLPL +V+ +EL +    K   +N  Q  V+ E P              ++ 
Sbjct: 254 AFVVVVLLLLPLVIVIKEELSVWTGKKQASNNHSQVNVVTENPPAVPLRPAEATIWPLAC 313

Query: 307 YKNIFNRPPPGEDHTILQAILNVDMLILFIATTCGVGGALTVVDNLAQIGASLHYPPRSI 366
            K IFN P  GED+ ILQAI ++DMLILFIAT CG GGALTV+DNL Q+G SL YP  SI
Sbjct: 314 IKTIFNPPERGEDYGILQAIFSIDMLILFIATACGTGGALTVIDNLGQLGKSLGYPKHSI 373

Query: 367 STFVSLLSIWNFLGRVVAGYASEFLLINYRLPRPLMLTAVILLSSFGHVLIAFAVPNSLY 426
           STF+SL+SIWNFLGRV+AGY SE  L NY  PRPLMLT VIL S  GH+LIAFAVPNSLY
Sbjct: 374 STFISLVSIWNFLGRVLAGYLSEIALTNYNFPRPLMLTLVILFSCIGHLLIAFAVPNSLY 433

Query: 427 LASMITGFSLGAQLPLTAIIISELFGLKHYSTLYNVGSVSSPVGSYIFNVRVAGRLYDRE 486
            AS++TGF LGAQLPL   IISELFGLKHYST+YNVGSVSSPVGSYIFNVRVAG LYD+E
Sbjct: 434 FASVMTGFCLGAQLPLLCAIISELFGLKHYSTMYNVGSVSSPVGSYIFNVRVAGHLYDKE 493

Query: 487 GERQNAEMGLVRKAGQELMCRGATCYRVSFLVMMAASAFGSLVSVVLVLRTRGFYRGDIY 536
             +Q   +GL RK GQ+L+C GATCYR++FL++  A+  GS+VS++LV RTR FY+GDIY
Sbjct: 494 ALKQMEALGLERKPGQDLICNGATCYRLAFLIISVATLLGSIVSLILVCRTRTFYKGDIY 553

BLAST of Lag0028069 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 4.9e-33
Identity = 132/533 (24.77%), Postives = 243/533 (45.59%), Query Frame = 0

Query: 15  RWLILFASLTIMAMNGSGYMFGLYSNHIKSFFGYDQSALNLVSFFKDLGANLGVLSGLLY 74
           +W +L A++ I A  G+ + F  YS+H+KS  G  Q  LN ++   DLG   G  SG+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 75  EVAPPWLVLSVGAALNFIAYFMVWAAISGRTAAPG--LWLMCLYMGVAANSLSFSNTAAL 134
              P  +VL   AA+ F+ Y + W  I+     P   ++L CL  G+   S+ + NTA  
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSLVFLCCLLAGL---SICWFNTACF 162

Query: 135 VTCVRNFPQYRGCLLGLLKGYIGLSGAIMTQLYHAMYGDDSKSLILLIAWLPAVVSLASL 194
           + C+R+FP  R   L L   + G+S A+ +  ++A+    S   +LL + +P VVS A+L
Sbjct: 163 ILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAAL 222

Query: 195 PVIRLINANNQQPNDDLKAFYALLYISLGLAASLIA--IIIAQTKLQFSKAEYIAAASPV 254
             +    + +  P+ D +   + ++  L + A + +  ++++ +    ++  +I A   +
Sbjct: 223 YPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVVLL 282

Query: 255 ILFLLLPLAVVVNQE-LKLPKTTVDNPPQSTVLVEPPQMSWYKNIF-------------- 314
           +  L  PL V      L +    +++     V++   ++   K                 
Sbjct: 283 VFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAKE 342

Query: 315 -NRPPPGEDHTILQAILNVDMLILFIATTCGVGGALTVVDNLAQIGASLHYPPRSISTFV 374
            N    G++H+    I  ++  + +IA  CG    L   +NL QI  SL    ++ +T V
Sbjct: 343 GNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSL---GQNSTTLV 402

Query: 375 SLLSIWNFLGRVVAGYASEFLLINYRLPRPLMLTAVILLSSFGHVLIAF--AVPNSLYLA 434
           ++ S ++F GR+++  A +F+   +RL R       +L +     L+A   +   +L  A
Sbjct: 403 TIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTA 462

Query: 435 SMITGFSLGAQLPLTAIIISELFGLKHYSTLYNVGSVSSPVGSYIFNVRVAGRLYDREGE 494
           + + G S G        I S+LFG       +N+   + P+GS ++   +A  +Y+    
Sbjct: 463 TALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY-IAASIYE---- 522

Query: 495 RQNAEMGLVRKAGQELMCRGATCYRVSFLVMMAASAFGSLVSVVLVLRTRGFY 526
             NA   +       ++C G  CY  +F+     S  G + S+ L +RT+  Y
Sbjct: 523 -ANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of Lag0028069 vs. ExPASy Swiss-Prot
Match: Q96TW9 (Probable transporter MCH1 OS=Wickerhamomyces anomalus OX=4927 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 5.5e-08
Identity = 42/171 (24.56%), Postives = 87/171 (50.88%), Query Frame = 0

Query: 300 PGEDHTILQAILNVDMLILFIATTCGVGGALTVVDNLAQIGASLHYPPRSISTFVSLLSI 359
           P      L+ I ++   +L  +    +G +   + N+  +  ++  P   IS  V++ ++
Sbjct: 249 PNHKSKFLKFIKDISTYVLLFSLLLSIGPSEMYITNMGSLVKAI-TPNSLISDQVAIHAV 308

Query: 360 WNFLGRVVAGYASEFLLINYRLPRPLMLTAVILLSSFGHVLIAFA--VPNSLYLASMITG 419
           ++ L R+  G  S+FL+ NY++ R  +L ++I+L  F  + IA +  V +  Y+ S ++G
Sbjct: 309 FSTLSRLSLGALSDFLVTNYQISRSWLLLSIIVLGFFTQIFIATSTFVKDQYYIISALSG 368

Query: 420 FSLGAQLPLTAIIISELFGLKHYSTLYNVGSVSSPVGSYIFNVRVAGRLYD 469
           FS G    L   +I  ++G + + + +    ++  +GS  F + V G +YD
Sbjct: 369 FSYGGLFTLYPTVIFSIWGPEIFGSAWGSFMIAPAIGSTTFGM-VFGLVYD 417

BLAST of Lag0028069 vs. ExPASy TrEMBL
Match: A0A0A0L178 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G507390 PE=4 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 2.2e-201
Identity = 379/485 (78.14%), Postives = 417/485 (85.98%), Query Frame = 0

Query: 1   MAAAVGFCLKLLTGRWLILFASLTIMAMNGSGYMFGLYSNHIKSFFGYDQSALNLVSFFK 60
           +AA  GF  KLLTGRWL+LFASL IMAMNGSGYMFGLYSNHIKS FGYDQS LNL+SFFK
Sbjct: 6   LAAVTGFLHKLLTGRWLMLFASLIIMAMNGSGYMFGLYSNHIKSVFGYDQSTLNLISFFK 65

Query: 61  DLGANLGVLSGLLYEVAPPWLVLSVGAALNFIAYFMVWAAISGRTAAPGLWLMCLYMGVA 120
           DLGANLGV+SGLLYEVAPPWLVLSVGA LNF  YFM+W A+SGRTAAPGL LMC+YM VA
Sbjct: 66  DLGANLGVVSGLLYEVAPPWLVLSVGAILNFFGYFMLWLAVSGRTAAPGLRLMCVYMSVA 125

Query: 121 ANSLSFSNTAALVTCVRNFPQYRGCLLGLLKGYIGLSGAIMTQLYHAMYGDDS-KSLILL 180
           ANSLSF NTAALVTC+RNFP +RGCLLGLLKGYIGLSGAIMTQLYHAMYG+D+ + LIL+
Sbjct: 126 ANSLSFGNTAALVTCLRNFPLHRGCLLGLLKGYIGLSGAIMTQLYHAMYGEDNPEGLILM 185

Query: 181 IAWLPAVVSLASLPVIRLINANNQQPNDDLKAFYALLYISLGLAASLIAIIIAQTKLQFS 240
           IAWLP+ +SLASLP IRLIN+NN Q N DLK FY LLYISL LAASL+AIII QTK  FS
Sbjct: 186 IAWLPSAISLASLPFIRLINSNNNQRN-DLKPFYNLLYISLALAASLLAIIIPQTKTHFS 245

Query: 241 KAEYIAAASPVILFLLLPLAVVVNQELKLPKTTVDNPPQSTVLVEPP---------QMSW 300
           K +YIA ASP++LFLLLPLAVVVNQEL L       PP +++LV+ P           +W
Sbjct: 246 KTDYIAVASPIVLFLLLPLAVVVNQELTLHN---HPPPITSILVQSPSPQLTTMSRSSNW 305

Query: 301 YKNIF-NRPPPGEDHTILQAILNVDMLILFIATTCGVGGALTVVDNLAQIGASLHYPPRS 360
           YKNIF  RP  G+DHTILQAIL+VDM ILF+ TTCGVGGALTVVDN+AQIGASL YP RS
Sbjct: 306 YKNIFTGRPMLGDDHTILQAILSVDMAILFVVTTCGVGGALTVVDNVAQIGASLDYPTRS 365

Query: 361 ISTFVSLLSIWNFLGRVVAGYASEFLLINYRLPRPLMLTAVILLSSFGHVLIAFAVPNSL 420
           IS+FVSL+SIWNFLGRV+AGY SEFLLI YRLPRPLMLT VILLS  GH++IAF VPNSL
Sbjct: 366 ISSFVSLMSIWNFLGRVMAGYVSEFLLIKYRLPRPLMLTFVILLSCIGHIMIAFGVPNSL 425

Query: 421 YLASMITGFSLGAQLPLTAIIISELFGLKHYSTLYNVGSVSSPVGSYIFNVRVAGRLYDR 475
           Y AS+ITGF LGAQLPLTA IIS+LFGLKHYSTLYNVGSVSSPVGSYIFNVR+AGR+YDR
Sbjct: 426 YFASIITGFCLGAQLPLTATIISDLFGLKHYSTLYNVGSVSSPVGSYIFNVRLAGRIYDR 485

BLAST of Lag0028069 vs. ExPASy TrEMBL
Match: A0A5D3C4Y9 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001510 PE=4 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 2.1e-199
Identity = 377/484 (77.89%), Postives = 415/484 (85.74%), Query Frame = 0

Query: 2   AAAVGFCLKLLTGRWLILFASLTIMAMNGSGYMFGLYSNHIKSFFGYDQSALNLVSFFKD 61
           AAA GF  KLLTGRWL+LFASL IMAMNGSGYMFGLYSNHIKS FGYDQSALN +SFFKD
Sbjct: 7   AAATGFLHKLLTGRWLMLFASLIIMAMNGSGYMFGLYSNHIKSIFGYDQSALNSISFFKD 66

Query: 62  LGANLGVLSGLLYEVAPPWLVLSVGAALNFIAYFMVWAAISGRTAAPGLWLMCLYMGVAA 121
           LGANLGV+SGLLYEVAPPWLVLSVGA LNF  YFM+W A+SGRTAAPGLWLMC+YMGVAA
Sbjct: 67  LGANLGVVSGLLYEVAPPWLVLSVGAILNFFGYFMLWLAVSGRTAAPGLWLMCVYMGVAA 126

Query: 122 NSLSFSNTAALVTCVRNFPQYRGCLLGLLKGYIGLSGAIMTQLYHAMYGDDS-KSLILLI 181
           NSLSF NTAALVTC+RNFP +RGCLLGLLKGYIGLSGAIMTQLYHAMYG+++ + LIL+I
Sbjct: 127 NSLSFGNTAALVTCLRNFPLHRGCLLGLLKGYIGLSGAIMTQLYHAMYGENNPEGLILMI 186

Query: 182 AWLPAVVSLASLPVIRLINANNQQPNDDLKAFYALLYISLGLAASLIAIIIAQTKLQFSK 241
           AWLP+ +SLASLP IR IN+NN Q N DLK FY LLYISL LAASL+AIII QTK+ FSK
Sbjct: 187 AWLPSAISLASLPFIRHINSNNNQRN-DLKPFYNLLYISLALAASLLAIIIPQTKIHFSK 246

Query: 242 AEYIAAASPVILFLLLPLAVVVNQELKLPKTTVDNPPQSTVLVEPP---------QMSWY 301
            +YIA AS VILFLLLPLA+VVNQEL L       PP +++LV+             +WY
Sbjct: 247 TDYIAVASLVILFLLLPLAIVVNQELTLHN---HPPPITSILVQSSSPHLTTMSRSSNWY 306

Query: 302 KNIF-NRPPPGEDHTILQAILNVDMLILFIATTCGVGGALTVVDNLAQIGASLHYPPRSI 361
           KNIF  RP  G+DHTILQAIL+VDM ILF+ATTCGVGG LTVVDN+AQIGASL YP RSI
Sbjct: 307 KNIFTGRPVFGDDHTILQAILSVDMAILFMATTCGVGGVLTVVDNVAQIGASLDYPTRSI 366

Query: 362 STFVSLLSIWNFLGRVVAGYASEFLLINYRLPRPLMLTAVILLSSFGHVLIAFAVPNSLY 421
           S+FVSL+SIWNFLGRV+AGY SEFLL+ YR PRPLMLT VILLS  GH++IAF VPNSLY
Sbjct: 367 SSFVSLMSIWNFLGRVMAGYVSEFLLMKYRFPRPLMLTFVILLSCIGHIMIAFGVPNSLY 426

Query: 422 LASMITGFSLGAQLPLTAIIISELFGLKHYSTLYNVGSVSSPVGSYIFNVRVAGRLYDRE 475
             S+ITGF LGAQLPLTA IIS+LFGLKHYSTLYNVGSVSSPVGSYIFNVRVAGR+YDRE
Sbjct: 427 FVSIITGFCLGAQLPLTATIISDLFGLKHYSTLYNVGSVSSPVGSYIFNVRVAGRVYDRE 486

BLAST of Lag0028069 vs. ExPASy TrEMBL
Match: A0A2P5FVE9 (Major facilitator OS=Trema orientale OX=63057 GN=TorMFS9 PE=4 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 1.2e-178
Identity = 340/577 (58.93%), Postives = 426/577 (73.83%), Query Frame = 0

Query: 7   FCLKLLTGRWLILFASLTIMAMNGSGYMFGLYSNHIKSFFGYDQSALNLVSFFKDLGANL 66
           F + +L  +W ++FA+L IM++NGSGYMFGLYSN IKS  GYDQS LNL+SFFKDLG NL
Sbjct: 14  FVVHVLWSKWFMVFATLLIMSVNGSGYMFGLYSNDIKSTLGYDQSTLNLISFFKDLGGNL 73

Query: 67  GVLSGLLYEVAPPWLVLSVGAALNFIAYFMVWAAISGRTAAPGLWLMCLYMGVAANSLSF 126
           GV +GL+ EV+PPWLVLS+GAA+NF  YFM+W +I+GR A P +W MCLY+ + ANSLSF
Sbjct: 74  GVTAGLINEVSPPWLVLSIGAAMNFFGYFMIWTSITGRVAKPHVWQMCLYICIGANSLSF 133

Query: 127 SNTAALVTCVRNFPQYRGCLLGLLKGYIGLSGAIMTQLYHAMYGDDSKSLILLIAWLPAV 186
            NTAALV CV+NFP+ RG ++GLLKG IGLSGAI+TQLYHA+YGDDS++L+LLIAW P V
Sbjct: 134 PNTAALVACVKNFPESRGSVIGLLKGLIGLSGAILTQLYHAIYGDDSEALVLLIAWFPPV 193

Query: 187 VSLASLPVIRLINANNQQPNDDLKAFYALLYISLGLAASLIAIIIAQTKLQFSKAEYIAA 246
           VSL  L  IR++  + +QPN +L+ F   LYISLG+A SL+A+II Q +L F++ +Y A+
Sbjct: 194 VSLIFLRTIRIMK-SVRQPN-ELRVFCNFLYISLGVAGSLMAMIIIQNRLHFNRVQYAAS 253

Query: 247 ASPVILFLLLPLAVVVNQELK-------LPKTTVDNPP---------QSTVLVEP----- 306
           AS +++ L L L VV+N+ELK         K   +NPP         Q+ +  EP     
Sbjct: 254 ASVILVVLFLSLFVVINEELKSWKLKKQALKEPSENPPISAPLPVIAQNPLASEPQPVIN 313

Query: 307 --------PQMSWYKNIFNRPPPGEDHTILQAILNVDMLILFIATTCGVGGALTVVDNLA 366
                   P +SW K IF  P  GED+TILQA L+ DMLILFIATTCGVGG LT++DNL 
Sbjct: 314 RVPPSSLKPYLSWVKTIFKAPDRGEDYTILQAALSTDMLILFIATTCGVGGTLTLIDNLG 373

Query: 367 QIGASLHYPPRSISTFVSLLSIWNFLGRVVAGYASEFLLINYRLPRPLMLTAVILLSSFG 426
           Q+G+SL YP  SI+TF+SL+SIWNFLGRV AG+ SE LL  Y+ PRP MLTAVILLS  G
Sbjct: 374 QLGSSLGYPKHSIATFISLISIWNFLGRVAAGFVSEILLTKYKFPRPSMLTAVILLSCVG 433

Query: 427 HVLIAFAVPNSLYLASMITGFSLGAQLPLTAIIISELFGLKHYSTLYNVGSVSSPVGSYI 486
           H+LIAF+VP+SLY  S+I+GF  GAQL L   IISELFGLK+YSTLYNVG++SSP+GSYI
Sbjct: 434 HLLIAFSVPHSLYFTSIISGFCYGAQLSLLFAIISELFGLKYYSTLYNVGALSSPLGSYI 493

Query: 487 FNVRVAGRLYDREGERQNAEMGLVRKAGQELMCRGATCYRVSFLVMMAASAFGSLVSVVL 546
            NVRVAGRLYD+E  +Q   +GLVRKAGQEL C G  CYR +F+V+ A + FGSLVS +L
Sbjct: 494 LNVRVAGRLYDKEALKQMEALGLVRKAGQELTCNGGKCYRTAFIVITAVTLFGSLVSFIL 553

Query: 547 VLRTRGFYRGDIYGRFKKVQGENEEIAGTALSTPTQG 555
           VLRTR FY+ DIY +F++   E   I  T ++    G
Sbjct: 554 VLRTRKFYKSDIYKKFRE---EERAITETQIAPTGNG 585

BLAST of Lag0028069 vs. ExPASy TrEMBL
Match: A0A5J5UFW9 (Nodulin-like domain-containing protein OS=Gossypium barbadense OX=3634 GN=ES319_A09G155000v1 PE=4 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 1.5e-178
Identity = 329/534 (61.61%), Postives = 407/534 (76.22%), Query Frame = 0

Query: 7   FCLKLLTGRWLILFASLTIMAMNGSGYMFGLYSNHIKSFFGYDQSALNLVSFFKDLGANL 66
           F   +L+ R  ++FA+L IM+ NGSGYMFGLYSN IKS  GYDQ+ LNL+SFFKDLG NL
Sbjct: 14  FVFDVLSSRLFMVFATLIIMSANGSGYMFGLYSNDIKSTLGYDQTTLNLLSFFKDLGGNL 73

Query: 67  GVLSGLLYEVAPPWLVLSVGAALNFIAYFMVWAAISGRTAAPGLWLMCLYMGVAANSLSF 126
           G+LSGL+YEV PPW+VL +G+ +NF  YFM+W +++GR A P  W MCLYM +AANS SF
Sbjct: 74  GILSGLIYEVVPPWVVLCMGSLMNFFGYFMLWVSVTGRIAKPHAWQMCLYMWIAANSQSF 133

Query: 127 SNTAALVTCVRNFPQYRGCLLGLLKGYIGLSGAIMTQLYHAMYGDDSKSLILLIAWLPAV 186
            NT ALVTCV+NFP+ RG +LGLLKG+IGLSGA+MTQ YHA Y DDSK+LILL+AWLP +
Sbjct: 134 PNTGALVTCVKNFPENRGSVLGLLKGFIGLSGAVMTQYYHAFYTDDSKALILLLAWLPTI 193

Query: 187 VSLASLPVIRLINANNQQPNDDLKAFYALLYISLGLAASLIAIIIAQTKLQFSKAEYIAA 246
           V L  L  IR++         +L+ FY  LYI+LGLA  ++ +I+ Q K++F++ EY+A+
Sbjct: 194 VPLIFLRTIRILKVVRMA--RELEIFYRFLYIALGLAGFIMVLIVLQNKVRFTRTEYVAS 253

Query: 247 ASPVILFLLLPLAVVVNQELKL---PKTTVDNPPQSTVL--VEPPQMSWYKNIFNRPPPG 306
           A  VI+ LLLPL +V+ +EL +    K   +N  Q  V    + P  +  K IF+ P  G
Sbjct: 254 AFVVIVLLLLPLVIVIKEELSVWTGKKQASNNHSQVNVASGSDDPAAACIKTIFDPPERG 313

Query: 307 EDHTILQAILNVDMLILFIATTCGVGGALTVVDNLAQIGASLHYPPRSISTFVSLLSIWN 366
           ED+ ILQAI ++DMLILFIAT CG GGALTV+DNL Q+G SL YP  SISTF+SL+SIWN
Sbjct: 314 EDYGILQAIFSIDMLILFIATACGTGGALTVIDNLGQLGKSLGYPKHSISTFISLVSIWN 373

Query: 367 FLGRVVAGYASEFLLINYRLPRPLMLTAVILLSSFGHVLIAFAVPNSLYLASMITGFSLG 426
           F GRV+AGY SE  L NY  PRPLMLT VIL S  GH+LIAFAVPNSLY AS++TGF LG
Sbjct: 374 FFGRVLAGYLSEMALTNYNFPRPLMLTLVILSSCIGHLLIAFAVPNSLYFASVMTGFCLG 433

Query: 427 AQLPLTAIIISELFGLKHYSTLYNVGSVSSPVGSYIFNVRVAGRLYDREGERQNAEMGLV 486
           AQLPL   IISELFGLKHYST+YNVGSVSSPVGSYIFNVRVAG LYD+E  +Q   +GL 
Sbjct: 434 AQLPLLCAIISELFGLKHYSTMYNVGSVSSPVGSYIFNVRVAGHLYDKEALKQMEALGLE 493

Query: 487 RKAGQELMCRGATCYRVSFLVMMAASAFGSLVSVVLVLRTRGFYRGDIYGRFKK 536
           RKAGQ+L C GATCYR++FL++ AA+  GS+VS++LV RTR FY+GDIY +FK+
Sbjct: 494 RKAGQDLTCNGATCYRLAFLIISAATLLGSIVSLILVFRTRTFYKGDIYKKFKE 545

BLAST of Lag0028069 vs. ExPASy TrEMBL
Match: A0A5D2F9V9 (Nodulin-like domain-containing protein OS=Gossypium darwinii OX=34276 GN=ES288_A09G176300v1 PE=4 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 1.5e-178
Identity = 329/534 (61.61%), Postives = 407/534 (76.22%), Query Frame = 0

Query: 7   FCLKLLTGRWLILFASLTIMAMNGSGYMFGLYSNHIKSFFGYDQSALNLVSFFKDLGANL 66
           F   +L+ R  ++FA+L IM+ NGSGYMFGLYSN IKS  GYDQ+ LNL+SFFKDLG NL
Sbjct: 14  FVFDVLSSRLFMVFATLIIMSANGSGYMFGLYSNDIKSTLGYDQTTLNLLSFFKDLGGNL 73

Query: 67  GVLSGLLYEVAPPWLVLSVGAALNFIAYFMVWAAISGRTAAPGLWLMCLYMGVAANSLSF 126
           G+LSGL+YEV PPW+VL +G+ +NF  YFM+W +++GR A P  W MCLYM +AANS SF
Sbjct: 74  GILSGLIYEVVPPWVVLCMGSLMNFFGYFMLWVSVTGRIAKPHAWQMCLYMWIAANSQSF 133

Query: 127 SNTAALVTCVRNFPQYRGCLLGLLKGYIGLSGAIMTQLYHAMYGDDSKSLILLIAWLPAV 186
            NT ALVTCV+NFP+ RG +LGLLKG+IGLSGA+MTQ YHA Y DDSK+LILL+AWLP +
Sbjct: 134 PNTGALVTCVKNFPENRGSVLGLLKGFIGLSGAVMTQYYHAFYTDDSKALILLLAWLPTI 193

Query: 187 VSLASLPVIRLINANNQQPNDDLKAFYALLYISLGLAASLIAIIIAQTKLQFSKAEYIAA 246
           V L  L  IR++         +L+ FY  LYI+LGLA  ++ +I+ Q K++F++ EY+A+
Sbjct: 194 VPLIFLRTIRILKVVRMA--RELEIFYRFLYIALGLAGFIMVLIVLQNKVRFTRTEYVAS 253

Query: 247 ASPVILFLLLPLAVVVNQELKL---PKTTVDNPPQSTVL--VEPPQMSWYKNIFNRPPPG 306
           A  VI+ LLLPL +V+ +EL +    K   +N  Q  V    + P  +  K IF+ P  G
Sbjct: 254 AFVVIVLLLLPLVIVIKEELSVWTGKKQASNNHSQVNVASGSDDPAAACIKTIFDPPERG 313

Query: 307 EDHTILQAILNVDMLILFIATTCGVGGALTVVDNLAQIGASLHYPPRSISTFVSLLSIWN 366
           ED+ ILQAI ++DMLILFIAT CG GGALTV+DNL Q+G SL YP  SISTF+SL+SIWN
Sbjct: 314 EDYGILQAIFSIDMLILFIATACGTGGALTVIDNLGQLGKSLGYPKHSISTFISLVSIWN 373

Query: 367 FLGRVVAGYASEFLLINYRLPRPLMLTAVILLSSFGHVLIAFAVPNSLYLASMITGFSLG 426
           F GRV+AGY SE  L NY  PRPLMLT VIL S  GH+LIAFAVPNSLY AS++TGF LG
Sbjct: 374 FFGRVLAGYLSEMALTNYNFPRPLMLTLVILSSCIGHLLIAFAVPNSLYFASVMTGFCLG 433

Query: 427 AQLPLTAIIISELFGLKHYSTLYNVGSVSSPVGSYIFNVRVAGRLYDREGERQNAEMGLV 486
           AQLPL   IISELFGLKHYST+YNVGSVSSPVGSYIFNVRVAG LYD+E  +Q   +GL 
Sbjct: 434 AQLPLLCAIISELFGLKHYSTMYNVGSVSSPVGSYIFNVRVAGHLYDKEALKQMEALGLE 493

Query: 487 RKAGQELMCRGATCYRVSFLVMMAASAFGSLVSVVLVLRTRGFYRGDIYGRFKK 536
           RKAGQ+L C GATCYR++FL++ AA+  GS+VS++LV RTR FY+GDIY +FK+
Sbjct: 494 RKAGQDLTCNGATCYRLAFLIISAATLLGSIVSLILVFRTRTFYKGDIYKKFKE 545

BLAST of Lag0028069 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 579.3 bits (1492), Expect = 3.3e-165
Identity = 310/579 (53.54%), Postives = 406/579 (70.12%), Query Frame = 0

Query: 9   LKLLTGRWLILFASLTIMAMNGSGYMFGLYSNHIKSFFGYDQSALNLVSFFKDLGANLGV 68
           +++LTGRW + F SL IM+  G+ YMFG+YS  IK   GYDQ+ LNL+SFFKDLGAN+GV
Sbjct: 15  IQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGV 74

Query: 69  LSGLLYEVAPPWLVLSVGAALNFIAYFMVWAAISGRTAAPGLWLMCLYMGVAANSLSFSN 128
           L+GLL EV PPW +L +GA LNF  YFM+W A++ R + P +W MCLY+ V ANS SF+N
Sbjct: 75  LAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFAN 134

Query: 129 TAALVTCVRNFPQYRGCLLGLLKGYIGLSGAIMTQLYHAMYGDDSKSLILLIAWLPAVVS 188
           T +LVTCV+NFP+ RG +LG+LKGY+GLSGAI+TQLY A YG+D+K LIL+I WLPA+VS
Sbjct: 135 TGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIVS 194

Query: 189 LASLPVIRLINANNQQPNDDLKAFYALLYISLGLAASLIAIIIAQTKLQFSKAEYIAAAS 248
            A L  IR++    Q   ++LK FY  LYISLGLA  L+ +II      F+++E+  +A+
Sbjct: 195 FAFLRTIRIMKVKRQ--TNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAA 254

Query: 249 PVILFLLLPLAVVVNQELKLPK---TTVDNPPQSTVLVEPPQM----------------- 308
            VI+ LLLP+ VV+ +E KL K     +++P    V+ E P++                 
Sbjct: 255 VVIVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVV 314

Query: 309 ------SWYKNIFNRPPPGEDHTILQAILNVDMLILFIATTCGVGGALTVVDNLAQIGAS 368
                 S +  +FN P  G+D+TILQA+ +VDMLILF+AT CGVGG LT +DNL QIG S
Sbjct: 315 EKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNS 374

Query: 369 LHYPPRSISTFVSLLSIWNFLGRVVAGYASEFLLINYRLPRPLMLTAVILLSSFGHVLIA 428
           L YP RS+STFVSL+SIWN+ GRVV+G  SE  LI Y+ PRPLMLT V+LLS  GH+LIA
Sbjct: 375 LGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIA 434

Query: 429 FAVPNSLYLASMITGFSLGAQLPLTAIIISELFGLKHYSTLYNVGSVSSPVGSYIFNVRV 488
           F VP  LY+AS+I GF  GAQ PL   IISE+FGLK+YSTLYN GSV+SP+GSY+ NVRV
Sbjct: 435 FNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRV 494

Query: 489 AGRLYDREGERQNAEMGLVRKAGQELMCRGATCYRVSFLVMMAASAFGSLVSVVLVLRTR 548
           AG LYD E  +Q   +G  R  GQ+L C G +C+++SF+++ A + FG LVS+VLV+RT+
Sbjct: 495 AGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTK 554

Query: 549 GFYRGDIYGRFK-KVQGENEEIAGTALSTPTQGLTKEEK 561
            FY+ DIY +F+ K      E+A  A +  T  + KE+K
Sbjct: 555 KFYKSDIYKKFREKALAAEMEMAAPAAARST--VAKEDK 589

BLAST of Lag0028069 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 568.2 bits (1463), Expect = 7.5e-162
Identity = 301/562 (53.56%), Postives = 397/562 (70.64%), Query Frame = 0

Query: 5   VGFCLKLLTGRWLILFASLTIMAMNGSGYMFGLYSNHIKSFFGYDQSALNLVSFFKDLGA 64
           V F +    GRW ++FAS  IMA  G+ Y+FG YS  IKS  GYDQ+ LNL+ FFKDLGA
Sbjct: 7   VRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGA 66

Query: 65  NLGVLSGLLYEVAPPWLVLSVGAALNFIAYFMVWAAISGRTAAPGLWLMCLYMGVAANSL 124
           N+GVLSGL+ EV P W VL++G+A+NF+ YFM+W  ++G+ A P +W MCLY+ + ANS 
Sbjct: 67  NVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQ 126

Query: 125 SFSNTAALVTCVRNFPQYRGCLLGLLKGYIGLSGAIMTQLYHAMYGDDSKSLILLIAWLP 184
           +F+NT ALVTCV+NFP+ RG +LGLLKGY+GLSGAI TQLY A+YG DSKSLILLIAWLP
Sbjct: 127 NFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLP 186

Query: 185 AVVSLASLPVIRLINANNQQPNDDLKAFYALLYISLGLAASLIAIIIAQTKLQFSKAEYI 244
           A VSL  + +IR      Q+  ++L  FY  LYIS+ LA  L+A+ IA+ ++ FSKA Y 
Sbjct: 187 AAVSLVFVYLIREKKVVRQR--NELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYA 246

Query: 245 AAASPVILFLLLPLAVVVNQELK---LPKTTVDNPPQSTVLVEPPQ-------------- 304
           A+A+     L +PL V V QEL+   + K  ++ P  S V VE P+              
Sbjct: 247 ASATICCALLFVPLTVSVKQELEVWNMMKLPIEEP--SEVKVEKPKKELDLDQDKAAKVN 306

Query: 305 ------MSWYKNIFNRPPPGEDHTILQAILNVDMLILFIATTCGVGGALTVVDNLAQIGA 364
                  S +  +F+ PP GED+TILQA+L+ DM+ILF+AT CG+G +LT VDNL QIG 
Sbjct: 307 GEEKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGE 366

Query: 365 SLHYPPRSISTFVSLLSIWNFLGRVVAGYASEFLLINYRLPRPLMLTAVILLSSFGHVLI 424
           SL YP  ++S+FVSL+SIWN+ GRV +G+ SE+LL  Y+LPRPLM+T V+LLS  GH+LI
Sbjct: 367 SLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLI 426

Query: 425 AFAVPNSLYLASMITGFSLGAQLPLTAIIISELFGLKHYSTLYNVGSVSSPVGSYIFNVR 484
           AF VP S+Y+AS++ GFS GAQLPL   IISELFGLK+YSTL+N G ++SP+GSYI NVR
Sbjct: 427 AFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVR 486

Query: 485 VAGRLYDREGERQNAEMGLVRKAGQELMCRGATCYRVSFLVMMAASAFGSLVSVVLVLRT 544
           V G LYD+E  +Q    GL RK  ++L C G+ CY++ FL++ A + FG+LVS+ L +RT
Sbjct: 487 VTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRT 546

BLAST of Lag0028069 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 341.7 bits (875), Expect = 1.1e-93
Identity = 210/540 (38.89%), Postives = 310/540 (57.41%), Query Frame = 0

Query: 9   LKLLTGRWLILFASLTIMAMNGSGYMFGLYSNHIKSFFGYDQSALNLVSFFKDLGANLGV 68
           +++L  +W+ + AS+ I   +G+ Y FG+YS  +KS   YDQS L+ VS FKD+GAN GV
Sbjct: 1   MEILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGV 60

Query: 69  LSGLLYEVAP----------------PWLVLSVGAALNFIAYFMVWAAISGRTAAPGLWL 128
            SGLLY  A                 PW+VL+VGA   F  YF++WA+++G    P + L
Sbjct: 61  FSGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPL 120

Query: 129 MCLYMGVAANSLSFSNTAALVTCVRNFPQYRGCLLGLLKGYIGLSGAIMTQLYHAMYGDD 188
           MCL+M +AA S +F NTA +V+ V NF  Y G  +G++KG++GLSGAI+ QLY  +   D
Sbjct: 121 MCLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD 180

Query: 189 SKSLILLIAWLPAVVSLASLPVIRLINANNQQPNDDLKAFYALLYISLGLAASLIAIIIA 248
             S ILL+A  P V+SL  +P++R+   +     DD K    L  +SL +AA L+ III 
Sbjct: 181 PASFILLLAVTPTVLSLLVMPLVRIYETS---VADDKKHLNGLSAVSLIIAAYLMIIIIL 240

Query: 249 QTKLQFSKAEYIAAASPVILFLLLPLAVVVNQELKLPKTTVDNPPQSTVLVEPPQMSWYK 308
           +     S    I     +++ L LPL +    +    + TV  P   + L+  P+ +   
Sbjct: 241 KNTFGLSSWANIVTLVCLLVMLALPLLIARRAQRDGMEKTV--PHDYSPLISSPKATTSG 300

Query: 309 NIFNRPPP------GEDHTILQAILNVDMLILFIATTCGVGGALTVVDNLAQIGASLHYP 368
           N  +           E+  +LQA+  +   +LF+A  CG+G  L+ ++N+ QIG SL Y 
Sbjct: 301 NQSSEGDSKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRYS 360

Query: 369 PRSISTFVSLLSIWNFLGRVVAGYASEFLLINYRLPRPLMLTAVILLSSFGHVLIAFAVP 428
              I++ VSL SIWNFLGR  AGYAS+ LL     PRPL++ A +   S GH++IA    
Sbjct: 361 SVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGFQ 420

Query: 429 NSLYLASMITGFSLGAQLPLTAIIISELFGLKHYSTLYNVGSVSSPVGSYIFNVRVAGRL 488
            +LY+ S+I G   G+Q  L   I SELFG++H  T++N  SV+SP+GSYIF+VR+ G +
Sbjct: 421 GNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSVRLIGYI 480

Query: 489 YDREGERQNAEMGLVRKAGQELMCRGATCYRVSFLVMMAASAFGSLVSVVLVLRTRGFYR 527
           YD+              +G+   C G+ C+R+SF++M + + FG LV++VL  RT+  YR
Sbjct: 481 YDKTA------------SGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLYR 523

BLAST of Lag0028069 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 325.9 bits (834), Expect = 6.5e-89
Identity = 199/536 (37.13%), Postives = 305/536 (56.90%), Query Frame = 0

Query: 9   LKLLTGRWLILFASLTIMAMNGSGYMFGLYSNHIKSFFGYDQSALNLVSFFKDLGANLGV 68
           ++ +  +W+   AS+ I + +G+ Y F +YS+ +KS   YDQS L+ VS FKD+G   G+
Sbjct: 1   MERINTKWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGI 60

Query: 69  LSGLLYEV--------APPWLVLSVGAALNFIAYFMVWAAISGRTAAPGLWLMCLYMGVA 128
           +SG LY            PW+V+ VG    F+ +F +WA++ G  A P + LMCL++ +A
Sbjct: 61  ISGFLYTAMTSKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLA 120

Query: 129 ANSLSFSNTAALVTCVRNFPQYRGCLLGLLKGYIGLSGAIMTQLYHAMYGDDSK--SLIL 188
            +SL F NTA +VT  RNF QY G  +G+++G++GLSGAI+ QLYHA+ G +    + IL
Sbjct: 121 GHSLPFFNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFIL 180

Query: 189 LIAWLPAVVSLASLPVIRLINANNQQPNDDLKAFYALLYISLGLAASLIAIIIAQTKLQF 248
           L+A +P +V   ++P +R+          D K    L  IS+ +AA L+ +I  +  L  
Sbjct: 181 LLAIVPTLVMFLAMPFVRVYET---VTISDKKHLDGLSAISMIIAAYLMVVITVENVLGL 240

Query: 249 SKAEYIAAASPVILFLLLPLAVVVN--QELKLPKTTVDNPPQST-VLVEPPQMSWYKNIF 308
           S++  I +   V+L L  PL V V   +E +   +++D P   T  L++PP      NIF
Sbjct: 241 SRSMQIFSFILVLLLLASPLLVAVRALREKRQTLSSLDGPVLDTSALLDPPS----SNIF 300

Query: 309 NRPPPG-----EDHTILQAILNVDMLILFIATTCGVGGALTVVDNLAQIGASLHYPPRSI 368
              P G     ED  IL+A+  V+  +LF+A  CG+G     V+N+ QIG SL Y    +
Sbjct: 301 ---PDGDHLVAEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRYSSVQL 360

Query: 369 STFVSLLSIWNFLGRVVAGYASEFLLINYRLPRPLMLTAVILLSSFGHVLIAFAVPNSLY 428
           ++ VSL SIWNFLGR  AGY S+  L  +  PRP+ +   + + + GH+++A  V  SLY
Sbjct: 361 NSLVSLWSIWNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGVQGSLY 420

Query: 429 LASMITGFSLGAQLPLTAIIISELFGLKHYSTLYNVGSVSSPVGSYIFNVRVAGRLYDRE 488
             S++ G + G+Q  L   I SE+FG++H  T+Y   S++ P+GSYI +V+V G  YD+ 
Sbjct: 421 AGSVLIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGYFYDKV 480

Query: 489 GERQNAEMGLVRKAGQELMCRGATCYRVSFLVMMAASAFGSLVSVVLVLRTRGFYR 527
               +              C G+ C+R SF++M + + FGSLV+ VL  RT  FY+
Sbjct: 481 ASEDDNS------------CFGSQCFRTSFMIMASVALFGSLVASVLFFRTHKFYK 514

BLAST of Lag0028069 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 325.5 bits (833), Expect = 8.5e-89
Identity = 197/534 (36.89%), Postives = 309/534 (57.87%), Query Frame = 0

Query: 10  KLLTGRWLILFASLTIMAMNGSGYMFGLYSNHIKSFFGYDQSALNLVSFFKDLGANLGVL 69
           ++L  +W+ + AS+ I    G  Y FG+YS  +KS   YDQS L+ VS FKD+G N+GVL
Sbjct: 4   EILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVL 63

Query: 70  SGLLYEVAP--------------PWLVLSVGAALNFIAYFMVWAAISGRTAAPGLWLMCL 129
           SGL+Y  A               PW+V+ +GA LNF  YF++WA+++G    P + +MCL
Sbjct: 64  SGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCL 123

Query: 130 YMGVAANSLSFSNTAALVTCVRNFPQYRGCLLGLLKGYIGLSGAIMTQLYHAMYGDDSKS 189
           +M +AA SL+F NTA +V+ + NF  Y G  +G++KG++GLSGA++ QLY  +   D K+
Sbjct: 124 FMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPKT 183

Query: 190 LILLIAWLPAVVSLASLPVIRLINANNQQPNDDLKAFYALLYISLGLAASLIAIIIAQTK 249
            ILL+A +P+++S+  +P++R+   +     D+ K    L  +SL +AA L+  II ++ 
Sbjct: 184 FILLLAIVPSLLSVLVMPLVRVYKTSTV---DEKKHLDGLSTLSLIIAAYLMITIILKST 243

Query: 250 LQFSKAEYIAAASPVILFLLLPLAVVV---NQELKLPKTTVDNPPQSTVLVEPPQMSWYK 309
           L           + +++ L  PL V V      ++ P ++V +P     LV+  + +   
Sbjct: 244 LSLPSWANAVTLAVLLVLLSSPLLVAVRAHRDSIEKPLSSVYSP-----LVDNLEATTSG 303

Query: 310 NIFNRPPPGEDHTILQAILNVDMLILFIATTCGVGGALTVVDNLAQIGASLHYPPRSIST 369
            I       +   +LQA+ NVD  +LF+A  CG+G  ++ ++N+ QIG SL Y    I++
Sbjct: 304 EIL-MLDEDKSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIGESLRYTSVEINS 363

Query: 370 FVSLLSIWNFLGRVVAGYASEFLLINYRLPRPLMLTAVILLSSFGHVLIAFAVPNSLYLA 429
            ++L +IWNF+GR   GY S++LL     PRPL++   +   + GH++IA     +LY  
Sbjct: 364 LLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHLIIASGFQGNLYPG 423

Query: 430 SMITGFSLGAQLPLTAIIISELFGLKHYSTLYNVGSVSSPVGSYIFNVRVAGRLYDREGE 489
           S+I G   G+Q  L   I SELFG+KH  T+YN  S++SP+GSYIF+VR+ G +YDR   
Sbjct: 424 SIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFSVRLIGYIYDR--- 483

Query: 490 RQNAEMGLVRKAGQELMCRGATCYRVSFLVMMAASAFGSLVSVVLVLRTRGFYR 527
                       G+   C G  C+R++++V+ + +  G LVS VLV RT+  YR
Sbjct: 484 ---------TIIGEGNTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFRTKTIYR 516