Lag0027890 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0027890 vs. NCBI nr
Match: XP_018436177.1 (PREDICTED: uncharacterized protein LOC108808549 [Raphanus sativus])

HSP 1 Score: 83.6 bits (205), Expect = 4.6e-12
Identity = 56/214 (26.17%), Postives = 92/214 (42.99%), Query Frame = 0

Query: 42  EAPWKSLWSLNIFPRAKISMLKIINNAIPTNSNIAIRGISTNLGCFLCGGA--------- 101
           +A ++ +WSLNI P+ K  + + +NNA+P   N+A R I+ +  C  CG A         
Sbjct: 16  DAIYQRVWSLNISPKVKHFLWRCLNNALPVTDNMAHRHIANDRRCSRCGKAAETINHMLF 75

Query: 102 --------WSQPNTYS-----GGEEL---------------------SKATIILWVLWNS 161
                   W++ N ++       E L                     S A  ILW LW +
Sbjct: 76  QCHYARRLWAKANVHTPPPGIWAESLHTNIHWVLNLKQEYPKDQIDESLAPWILWRLWKN 135

Query: 162 RNNCRNSNQQLDINQVSRLLTNYIEELEDKNRPYLD-FPEVQSQPSLLSWKPPDEGWWKL 212
           RN      ++ +I++        ++E +DK    ++   +  ++     W PP   W K 
Sbjct: 136 RNEMLFRGKEYEISETVEKAWEDVKEWQDKKEAKVEVVKKTTTEKPDRKWIPPVPTWLKC 195

BLAST of Lag0027890 vs. NCBI nr
Match: XP_030924745.1 (uncharacterized protein LOC115951731 [Quercus lobata])

HSP 1 Score: 82.0 bits (201), Expect = 1.3e-11
Identity = 67/221 (30.32%), Postives = 97/221 (43.89%), Query Frame = 0

Query: 25   SVTTDKDKASPSNCGQAEAPWKSLWSLNIFPRAKISMLKIINNAIPTNSNIAIRGISTNL 84
            + T     +SP N  Q    W+ LW L +  + K    +  N+A+PT SN+  R I+TN 
Sbjct: 975  NATNHAGTSSPVNQRQF---WRKLWQLRVPNKLKHFAWRACNDALPTLSNLVRRHIATNE 1034

Query: 85   GCFLCGG----------------------AWSQP--NTYSGG-----------EELSKAT 144
             C  C                        +WSQP  N+++             +E  ++ 
Sbjct: 1035 ICSACQAQPEDTLHALWSCSTLESVWSPLSWSQPYANSHTSSFQGLLDKFMQVKEDYRSE 1094

Query: 145  I---ILWVLWNSRNNCRNSNQQLDINQVSRLLTNYIEELEDKNRPYLD-FPEVQSQPSLL 204
            I   I W+LWN RN  R +     IN +S L  +Y++E  D     LD  P V   PS  
Sbjct: 1095 IFITIAWMLWNRRNALRLNLTVQPINHISSLAGSYLQEFLDT----LDQIPVVMDHPSPQ 1154

Query: 205  SWKPPDEGWWKLNSDASWSSAAMRGGVGWVIRDSAGSLIGA 207
             W+PPDE  +K N DA+    +   G+G +IRD  G  +GA
Sbjct: 1155 QWQPPDESSFKANFDAAVFKNSNHAGIGVIIRDWRGEAVGA 1188

BLAST of Lag0027890 vs. NCBI nr
Match: XP_023880772.1 (uncharacterized protein LOC111993138 [Quercus suber])

HSP 1 Score: 82.0 bits (201), Expect = 1.3e-11
Identity = 58/219 (26.48%), Postives = 94/219 (42.92%), Query Frame = 0

Query: 48  LWSLNIFPRAKISMLKIINNAIPTNSNIAIRGISTNLGCFLCGGAWSQPNTYSG------ 107
           +W+LN   + K  + +   N +PT   +  RG+  ++GC  CG      +   G      
Sbjct: 99  VWNLNCPNKLKQFLWRSCRNILPTKHQLKSRGVDIDVGCDFCGICEFVEHVLWGCKFAAE 158

Query: 108 --GEELSKATII-----------------------------LWVLWNSRNNCRNSNQQLD 167
             GE   K  +I                             +W LWN RN  R   Q+  
Sbjct: 159 VWGESRLKLPLIPYPTEEFLEVVWEIRDKKPDIDWELFAATVWGLWNQRNTVRYGGQRKT 218

Query: 168 INQVSRLLTNYIEELEDKNRPYLDFPEVQSQPSLLSWKPPDEGWWKLNSDASWSSAAMRG 227
            ++++R +  Y+ E   +N P    P   S+P   +W PP +GW+K N D + S      
Sbjct: 219 AHRLTRDMEEYLREFRHENSP----PCKPSRPFAPTWTPPKQGWYKANMDGAVSKERDCC 278

Query: 228 GVGWVIRDSAGSLIGAGCSRVSRYWPIKALEVKVLPEEE 230
           G+G VIR++ G ++GA   R+    P+KALE + +  +E
Sbjct: 279 GIGVVIRNAEGQIMGAMSKRLE--LPLKALETEAMAIQE 311

BLAST of Lag0027890 vs. NCBI nr
Match: OMO85295.1 (reverse transcriptase [Corchorus capsularis])

HSP 1 Score: 78.6 bits (192), Expect = 1.5e-10
Identity = 59/212 (27.83%), Postives = 84/212 (39.62%), Query Frame = 0

Query: 39   GQAEAPWKSLWSLNIFPRAKISMLKIINNAIPTNSNIAIRGISTNLGCFLCGG------- 98
            GQ +  WK +WSL   P+ K  + ++I   +PT S +  RG   NL C  C         
Sbjct: 987  GQVDQFWKWVWSLKFLPKLKFFLWEVILGIVPTRSLLFHRGFIVNLSCPFCNSEEETLQH 1046

Query: 99   --------------------------AWSQPNTYSGGEELSKA-------TIILWVLWNS 158
                                       W + N        + +       T +LW +W  
Sbjct: 1047 LFRDCIVTQTFWQQAGFIFQPTSPFDLWLKDNLLRKDYHQNPSIPFSLLFTHLLWEIWLE 1106

Query: 159  RNNCRNSNQQLDINQVSRLLTNYIEELEDKNRPYLDFPEVQSQPSLLSWKPPDEGWWKLN 211
            RN     N ++  N VSR     +E      R       VQS  + +SWKPPD+GW+K+N
Sbjct: 1107 RNQVVFKNVRVRENLVSRAFNKAVEFFSLTGR----IKRVQSISAPVSWKPPDQGWFKVN 1166

BLAST of Lag0027890 vs. NCBI nr
Match: CAB4279811.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 78.2 bits (191), Expect = 1.9e-10
Identity = 60/211 (28.44%), Postives = 90/211 (42.65%), Query Frame = 0

Query: 45   WKSLWSLNIFPRAKISMLKIINNAIPTNSNIAIRGISTNLGCFLCGGAWSQPNTYSGGEE 104
            WKS+W L + P+ +  + K   N +    N+   GI     C LCG      +     E 
Sbjct: 936  WKSIWGLQVPPKIRTFLWKCCRNVLAVKENLLHWGIDVETTCALCGQEGESQDFIVCWEN 995

Query: 105  LSKATIILWVLWNSRNNCRNSNQ----------QLDINQVSRLLTNYIEE---LEDKNRP 164
            L K        +    +C +  Q          +  +NQV    T  +     + +  RP
Sbjct: 996  LLKK-------YEQVEHCNDILQGTVIEPHVAVECFLNQVHEFRTAQMHGKPLINELPRP 1055

Query: 165  YLDFPEVQSQPSLLSWKPPDEGWWKLNSDASWSSAAMRGGVGWVIRDSAGSLIGAGCSRV 224
                PE+Q++ ++ SW+    G  K+N DA+W S    GGVGWV+RDS G ++ AGC   
Sbjct: 1056 ----PEIQAE-TVTSWRKLPYGVLKVNCDAAWVSQTQMGGVGWVVRDSYGWMVQAGCKGD 1115

Query: 225  SRYWPIKALEVKVLPEEEEDYSDSGLIFAEI 243
             R     A+E K + E       SGL   E+
Sbjct: 1116 MRGGSALAMEAKAIREALLACVQSGLSKLEV 1134

BLAST of Lag0027890 vs. ExPASy TrEMBL
Match: A0A6J0JKM2 (uncharacterized protein LOC108808549 OS=Raphanus sativus OX=3726 GN=LOC108808549 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.2e-12
Identity = 56/214 (26.17%), Postives = 92/214 (42.99%), Query Frame = 0

Query: 42  EAPWKSLWSLNIFPRAKISMLKIINNAIPTNSNIAIRGISTNLGCFLCGGA--------- 101
           +A ++ +WSLNI P+ K  + + +NNA+P   N+A R I+ +  C  CG A         
Sbjct: 16  DAIYQRVWSLNISPKVKHFLWRCLNNALPVTDNMAHRHIANDRRCSRCGKAAETINHMLF 75

Query: 102 --------WSQPNTYS-----GGEEL---------------------SKATIILWVLWNS 161
                   W++ N ++       E L                     S A  ILW LW +
Sbjct: 76  QCHYARRLWAKANVHTPPPGIWAESLHTNIHWVLNLKQEYPKDQIDESLAPWILWRLWKN 135

Query: 162 RNNCRNSNQQLDINQVSRLLTNYIEELEDKNRPYLD-FPEVQSQPSLLSWKPPDEGWWKL 212
           RN      ++ +I++        ++E +DK    ++   +  ++     W PP   W K 
Sbjct: 136 RNEMLFRGKEYEISETVEKAWEDVKEWQDKKEAKVEVVKKTTTEKPDRKWIPPVPTWLKC 195

BLAST of Lag0027890 vs. ExPASy TrEMBL
Match: A0A803PA46 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.9e-11
Identity = 57/210 (27.14%), Postives = 93/210 (44.29%), Query Frame = 0

Query: 25   SVTTDKDKASPSNCGQAEAPWKSLWSLNIFPRAKISMLKIINNAIPTNSNIAIRGISTNL 84
            S T+  D  SPS+       WK+LW L++ P+ K  + +   N +PT  N+A+R I  N 
Sbjct: 1041 SSTSPPDIPSPSS---PSPWWKNLWLLHVPPKVKHFVYRASTNTLPTKKNLALRTILPNS 1100

Query: 85   GCFLCGGAWSQPNTYSGGEELSKAT---------IILWVLWNSRNNCRNSNQQLDINQVS 144
             C  C     Q  T S      +            I W++W++RN    +  Q   + + 
Sbjct: 1101 LCDRCD---HQEETVSHALFFCRNVRSVWKDSFLCISWLIWHNRNRALRNQAQDQNHAIP 1160

Query: 145  RLLTNYIEELEDKNRPYLDFPEVQSQPSLLSWKPPDEGWWKLNSDASWSSAAMRGGVGWV 204
             L  +++ E +  ++       V   P +  W+PP  G  KLN+DA+    + + G G V
Sbjct: 1161 SLANSFLAEYKTSSQASQSQGPVTRPPPVSVWEPPPNGCLKLNTDAAIPKNSAKAGFGGV 1220

Query: 205  IRDSAGSLIGAGCSRVSRYWPIKALEVKVL 226
            IR+S G ++ A  +       +  LE K L
Sbjct: 1221 IRNSEGQVVAAVANSHLGGGDVATLEAKAL 1244

BLAST of Lag0027890 vs. ExPASy TrEMBL
Match: A0A2N9H1N4 (RNase H domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS33433 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 5.5e-11
Identity = 56/233 (24.03%), Postives = 105/233 (45.06%), Query Frame = 0

Query: 30   KDKASPSNCGQAEAPWKSLWSLNIFPRAKISMLKIINNAIPTNSNIAIRGISTNLGCFLC 89
            ++++ PS+  Q    WKS+WSLN+ P+ +  + +  +N++PT SN+  R I  +  C +C
Sbjct: 1237 REESGPSDPSQMSQLWKSVWSLNVPPKTRHFLWRACHNSLPTRSNLHHRHILDDPRCTIC 1296

Query: 90   GG----------------------AWSQ---PNTYS-------------GGEELSKATII 149
                                    +W Q    +TY+                EL   ++I
Sbjct: 1297 SKQLESTIHALWQCKKVQSVWKSISWGQQLAESTYTDFIDLLHKCFSLLSTSELQLFSMI 1356

Query: 150  LWVLWNSRNNCR---NSNQQLDINQVSRLLTNYIEELEDKNRPYLDFPEVQSQPSLLSWK 209
             W +W  RN  R   + +  L + Q +R   +  +E +D+ +     P+  S  + + W 
Sbjct: 1357 CWSIWYRRNRLRLQQSVDNDLQLVQRARDSMSEFQEAQDREQ---QLPQQPSSSATVKWN 1416

Query: 210  PPDEGWWKLNSDASWSSAAMRGGVGWVIRDSAGSLIGAGCSRVSRYWPIKALE 222
            PP +G +K+N D +  S +   G+G +IR+  G ++G+   R      ++A+E
Sbjct: 1417 PPAQGRYKVNYDGAIFSDSNAAGIGVIIRNHQGEVMGSLSQRFPFPHSVEAVE 1466

BLAST of Lag0027890 vs. ExPASy TrEMBL
Match: A0A1R3IRS5 (Reverse transcriptase OS=Corchorus capsularis OX=210143 GN=CCACVL1_10289 PE=4 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 7.2e-11
Identity = 59/212 (27.83%), Postives = 84/212 (39.62%), Query Frame = 0

Query: 39   GQAEAPWKSLWSLNIFPRAKISMLKIINNAIPTNSNIAIRGISTNLGCFLCGG------- 98
            GQ +  WK +WSL   P+ K  + ++I   +PT S +  RG   NL C  C         
Sbjct: 987  GQVDQFWKWVWSLKFLPKLKFFLWEVILGIVPTRSLLFHRGFIVNLSCPFCNSEEETLQH 1046

Query: 99   --------------------------AWSQPNTYSGGEELSKA-------TIILWVLWNS 158
                                       W + N        + +       T +LW +W  
Sbjct: 1047 LFRDCIVTQTFWQQAGFIFQPTSPFDLWLKDNLLRKDYHQNPSIPFSLLFTHLLWEIWLE 1106

Query: 159  RNNCRNSNQQLDINQVSRLLTNYIEELEDKNRPYLDFPEVQSQPSLLSWKPPDEGWWKLN 211
            RN     N ++  N VSR     +E      R       VQS  + +SWKPPD+GW+K+N
Sbjct: 1107 RNQVVFKNVRVRENLVSRAFNKAVEFFSLTGR----IKRVQSISAPVSWKPPDQGWFKVN 1166

BLAST of Lag0027890 vs. ExPASy TrEMBL
Match: A0A6J5UV01 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS32434 PE=4 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 9.4e-11
Identity = 60/211 (28.44%), Postives = 90/211 (42.65%), Query Frame = 0

Query: 45   WKSLWSLNIFPRAKISMLKIINNAIPTNSNIAIRGISTNLGCFLCGGAWSQPNTYSGGEE 104
            WKS+W L + P+ +  + K   N +    N+   GI     C LCG      +     E 
Sbjct: 936  WKSIWGLQVPPKIRTFLWKCCRNVLAVKENLLHWGIDVETTCALCGQEGESQDFIVCWEN 995

Query: 105  LSKATIILWVLWNSRNNCRNSNQ----------QLDINQVSRLLTNYIEE---LEDKNRP 164
            L K        +    +C +  Q          +  +NQV    T  +     + +  RP
Sbjct: 996  LLKK-------YEQVEHCNDILQGTVIEPHVAVECFLNQVHEFRTAQMHGKPLINELPRP 1055

Query: 165  YLDFPEVQSQPSLLSWKPPDEGWWKLNSDASWSSAAMRGGVGWVIRDSAGSLIGAGCSRV 224
                PE+Q++ ++ SW+    G  K+N DA+W S    GGVGWV+RDS G ++ AGC   
Sbjct: 1056 ----PEIQAE-TVTSWRKLPYGVLKVNCDAAWVSQTQMGGVGWVVRDSYGWMVQAGCKGD 1115

Query: 225  SRYWPIKALEVKVLPEEEEDYSDSGLIFAEI 243
             R     A+E K + E       SGL   E+
Sbjct: 1116 MRGGSALAMEAKAIREALLACVQSGLSKLEV 1134

BLAST of Lag0027890 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 3.5e-10
Identity = 48/226 (21.24%), Postives = 91/226 (40.27%), Query Frame = 0

Query: 45  WKSLWSLNIFPRAKISMLKIINNAIPTNSNIAIRGISTNLGCFLC--------------- 104
           ++ +W     P+ +  + K ++N++P    +A R +S    C  C               
Sbjct: 254 YQKIWKSQTSPKIQHFLWKCLSNSLPVAGALAYRHLSKESACIRCPSCKETVNHLLFKCT 313

Query: 105 ---------------GGAWSQP---------NTYSGGEELSKAT----IILWVLWNSRNN 164
                          GG W+           N  +G  +  KA+     +LW LW +RN 
Sbjct: 314 FARLTWAISSIPIPLGGEWADSIYVNLYWVFNLGNGNPQWEKASQLVPWLLWRLWKNRNE 373

Query: 165 CRNSNQQLDINQVSRLLTNYIEE--LEDKNRPYLDFPEVQSQPSLLSWKPPDEGWWKLNS 224
                ++ +  +V R   + +EE  +  +       P+V ++ S   W+PP   W K N+
Sbjct: 374 LVFRGREFNAQEVLRRAEDDLEEWRIRTEAESCGTKPQV-NRSSCGRWRPPPHQWVKCNT 433

Query: 225 DASWSSAAMRGGVGWVIRDSAGSLIGAGCSRVSRYWPIKALEVKVL 226
           DA+W+    R G+GWV+R+  G +   G   + +   +   E++ +
Sbjct: 434 DATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAM 478

BLAST of Lag0027890 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 58.5 bits (140), Expect = 1.5e-08
Identity = 34/117 (29.06%), Postives = 55/117 (47.01%), Query Frame = 0

Query: 111 ILWVLWNSRNNCRNSNQQLDINQVSRLLTNYIEELEDKN--RPYLDFPEVQSQPSLLSWK 170
           +LW LW SRN      ++ D  +V R      EE   +         P+V+   S + WK
Sbjct: 80  LLWRLWKSRNELMFKGKEYDAPEVLRRAMEDFEEWSTRRELEGKASGPQVERNLS-VQWK 139

Query: 171 PPDEGWWKLNSDASWSSAAMRGGVGWVIRDSAGSLIGAGCSRVSRYWPIKALEVKVL 226
            P   W K N+DA+W     R G+GW++R+ +G ++  G   + R   +   E++ L
Sbjct: 140 APPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAELEAL 195

BLAST of Lag0027890 vs. TAIR 10
Match: AT4G09775.1 (BEST Arabidopsis thaliana protein match is: Ribonuclease H-like superfamily protein (TAIR:AT2G02650.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 45.1 bits (105), Expect = 1.7e-04
Identity = 33/122 (27.05%), Postives = 55/122 (45.08%), Query Frame = 0

Query: 112 LWVLWNSRNNCRNSNQQLD------INQVSRLLTNYIEEL--EDKNRPYLDFPEVQSQPS 171
           +W LW SRN      QQ+D        +  +  T ++E    +  N    + P  +    
Sbjct: 1   MWRLWKSRNEF--LFQQIDRFPWKVAQKGEQEATEWVETTINDTANSHSTEQPNDRPSGR 60

Query: 172 LLSWKPPDEGWWKLNSDASWSSAAMRGGVGWVIRDSAGSLIGAGCSRVSRYWPIKALEVK 226
              W PP EG+ K N D+ +          W+IRDS G +I +GC+++ + +   AL+ +
Sbjct: 61  SKEWSPPPEGYLKCNFDSGYVQGRDYTSTCWIIRDSNGHVIHSGCAKLQQSY--SALQAE 118