Lag0027806 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0027806
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr8: 5352689 .. 5353600 (-)
RNA-Seq ExpressionLag0027806
SyntenyLag0027806
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACTCATGCATCTTTCGATCAACAATTATTGGGACATAGTGGGGGAAGACACATTGGAGGTATGCCTCAAAATCCTAAATGAGGACGCAGACATAACCCCCCTAAACAAAACCTTGATTGCACTCATCCCTAAAGTCAAAGAAGCAAAACATATGCATGATTTTCGGCCAATTAGCCTGTGCAATGTGAGCTACAAGATCATTGCAAAGGCTATTGTAAACCGGTTCAAGAAAGTGTTAGATAAGATTACCTCCCCTTCACAGGCGGCGTTTGTCCCAAGAAGGCTAATCTCAGATAATGTGATCACCGGCTTTGAGTGCATACATGCTATAAATAACAAAAAGAAGGGCAAAGAAGGTCAAGTGGCTATTAAACTCGATATGAGCAAGTGCTTCATTAGAGAGATGTTGATAAAGCTGGGATTCAGTGAAAGATGGACAAAGAACATAATGAGATGTGTGGAAACCGTAACTTTCTCAGTTCTAACAAATGGCACCCCACAAGAAGAGTTTGCTCCAAAGCGAGGAATCAGACAAGGAGATCCACTATCACCATACCTATTCCTCATTTGCGCGGAAGGATTCTTGAGACTCCTGTCCAGGGAAGTTGAAACTCATAACTTTAAGGGTTTTCGTATTAATAATCATTGTCCTACGCTCTCTCACTTATTTTTCGCAGATGATAGTCTCATTTTTTGCAGGGAAAACACAAAGGAATGTCAAGTACTCAAAGAGATATTAAAAAATTACAAGGAAGCATCAGACCAAAAGATAAATTTGGATAAGTCTGCATTCATGTTAAGTAAGAACGTGAAGCAGGAGGAAGGCAGAATCGTCGAAGAGATCCTTGAAAAAAAAAAAAAGAACAAAGGCTCTGGGCAATTATTTGGGAATGCCATCCCAAAATAG

mRNA sequence

ATGGCACTCATGCATCTTTCGATCAACAATTATTGGGACATAGTGGGGGAAGACACATTGGAGGTATGCCTCAAAATCCTAAATGAGGACGCAGACATAACCCCCCTAAACAAAACCTTGATTGCACTCATCCCTAAAGTCAAAGAAGCAAAACATATGCATGATTTTCGGCCAATTAGCCTGTGCAATGTGAGCTACAAGATCATTGCAAAGGCTATTGTAAACCGGTTCAAGAAAGTGTTAGATAAGATTACCTCCCCTTCACAGGCGGCGTTTGTCCCAAGAAGGCTAATCTCAGATAATGTGATCACCGGCTTTGAGTGCATACATGCTATAAATAACAAAAAGAAGGGCAAAGAAGGTCAAGTGGCTATTAAACTCGATATGAGCAAGTGCTTCATTAGAGAGATGTTGATAAAGCTGGGATTCAGTGAAAGATGGACAAAGAACATAATGAGATGTGTGGAAACCGTAACTTTCTCAGTTCTAACAAATGGCACCCCACAAGAAGAGTTTGCTCCAAAGCGAGGAATCAGACAAGGAGATCCACTATCACCATACCTATTCCTCATTTGCGCGGAAGGATTCTTGAGACTCCTGTCCAGGGAAGTTGAAACTCATAACTTTAAGGGTTTTCGTATTAATAATCATTGTCCTACGCTCTCTCACTTATTTTTCGCAGATGATAGTCTCATTTTTTGCAGGGAAAACACAAAGGAATGTCAAGTACTCAAAGAGATATTAAAAAATTACAAGGAAGCATCAGACCAAAAGATAAATTTGGATAAGTCTGCATTCATGTTAAGTAAGAACGTGAAGCAGGAGGAAGGCAGAATCGTCGAAGAGATCCTTGAAAAAAAAAAAAAGAACAAAGGCTCTGGGCAATTATTTGGGAATGCCATCCCAAAATAG

Coding sequence (CDS)

ATGGCACTCATGCATCTTTCGATCAACAATTATTGGGACATAGTGGGGGAAGACACATTGGAGGTATGCCTCAAAATCCTAAATGAGGACGCAGACATAACCCCCCTAAACAAAACCTTGATTGCACTCATCCCTAAAGTCAAAGAAGCAAAACATATGCATGATTTTCGGCCAATTAGCCTGTGCAATGTGAGCTACAAGATCATTGCAAAGGCTATTGTAAACCGGTTCAAGAAAGTGTTAGATAAGATTACCTCCCCTTCACAGGCGGCGTTTGTCCCAAGAAGGCTAATCTCAGATAATGTGATCACCGGCTTTGAGTGCATACATGCTATAAATAACAAAAAGAAGGGCAAAGAAGGTCAAGTGGCTATTAAACTCGATATGAGCAAGTGCTTCATTAGAGAGATGTTGATAAAGCTGGGATTCAGTGAAAGATGGACAAAGAACATAATGAGATGTGTGGAAACCGTAACTTTCTCAGTTCTAACAAATGGCACCCCACAAGAAGAGTTTGCTCCAAAGCGAGGAATCAGACAAGGAGATCCACTATCACCATACCTATTCCTCATTTGCGCGGAAGGATTCTTGAGACTCCTGTCCAGGGAAGTTGAAACTCATAACTTTAAGGGTTTTCGTATTAATAATCATTGTCCTACGCTCTCTCACTTATTTTTCGCAGATGATAGTCTCATTTTTTGCAGGGAAAACACAAAGGAATGTCAAGTACTCAAAGAGATATTAAAAAATTACAAGGAAGCATCAGACCAAAAGATAAATTTGGATAAGTCTGCATTCATGTTAAGTAAGAACGTGAAGCAGGAGGAAGGCAGAATCGTCGAAGAGATCCTTGAAAAAAAAAAAAAGAACAAAGGCTCTGGGCAATTATTTGGGAATGCCATCCCAAAATAG

Protein sequence

MALMHLSINNYWDIVGEDTLEVCLKILNEDADITPLNKTLIALIPKVKEAKHMHDFRPISLCNVSYKIIAKAIVNRFKKVLDKITSPSQAAFVPRRLISDNVITGFECIHAINNKKKGKEGQVAIKLDMSKCFIREMLIKLGFSERWTKNIMRCVETVTFSVLTNGTPQEEFAPKRGIRQGDPLSPYLFLICAEGFLRLLSREVETHNFKGFRINNHCPTLSHLFFADDSLIFCRENTKECQVLKEILKNYKEASDQKINLDKSAFMLSKNVKQEEGRIVEEILEKKKKNKGSGQLFGNAIPK
Homology
BLAST of Lag0027806 vs. NCBI nr
Match: XP_023881891.1 (uncharacterized protein LOC111994244 [Quercus suber])

HSP 1 Score: 282.3 bits (721), Expect = 4.8e-72
Identity = 133/273 (48.72%), Postives = 187/273 (68.50%), Query Frame = 0

Query: 11  YWDIVGEDTLEVCLKILNEDADITPLNKTLIALIPKVKEAKHMHDFRPISLCNVSYKIIA 70
           YW+IVG D + + L +LN +  +  +NKT I L+PK+K    M DFRPISLCNV YK+I+
Sbjct: 299 YWNIVGNDIVCMVLDVLNSNMSMVEINKTNITLVPKIKNPTKMSDFRPISLCNVVYKLIS 358

Query: 71  KAIVNRFKKVLDKITSPSQAAFVPRRLISDNVITGFECIHAINNKKKGKEGQVAIKLDMS 130
           K + NR K +L +I S +Q+AF+  RLI+DNV+  FE +H + +KK+GKEG  AIKLDMS
Sbjct: 359 KVLANRLKNILPQIISENQSAFLSGRLITDNVLVAFELMHYLEHKKEGKEGFAAIKLDMS 418

Query: 131 KC-------FIREMLIKLGFSERWTKNIMRCVETVTFSVLTNGTPQEEFAPKRGIRQGDP 190
           K        FI++++ K+GF E+W K +M C+ +V++S+L NG       P RG+RQGDP
Sbjct: 419 KAYDRVEWGFIKQVMEKMGFHEKWIKLVMHCITSVSYSILVNGGAYGSITPTRGLRQGDP 478

Query: 191 LSPYLFLICAEGFLRLLSREVETHNFKGFRINNHCPTLSHLFFADDSLIFCRENTKECQV 250
           +SPY+FL+CA+GF  LL+         G  I   CP ++HLFFADDSL+FC+ N++ECQ 
Sbjct: 479 ISPYIFLLCADGFSSLLNDVARKLRISGVSICRGCPKITHLFFADDSLLFCKANSQECQT 538

Query: 251 LKEILKNYKEASDQKINLDKSAFMLSKNVKQEE 277
           L +IL+ Y++AS QKIN+DKS+   S N   E+
Sbjct: 539 LIDILQLYEDASGQKINVDKSSVFFSNNTPDEK 571

BLAST of Lag0027806 vs. NCBI nr
Match: XP_023901742.1 (uncharacterized protein LOC112013579 [Quercus suber])

HSP 1 Score: 281.6 bits (719), Expect = 8.2e-72
Identity = 142/295 (48.14%), Postives = 185/295 (62.71%), Query Frame = 0

Query: 9   NNYWDIVGEDTLEVCLKILNEDADITPLNKTLIALIPKVKEAKHMHDFRPISLCNVSYKI 68
           +NYWDIVG     + L +LN +  +T +NKT I+LIPK  +   M +FRPISLCN +YKI
Sbjct: 306 HNYWDIVGPSITNMVLNVLNSNLPMTEINKTNISLIPKTNQPVRMTEFRPISLCNTTYKI 365

Query: 69  IAKAIVNRFKKVLDKITSPSQAAFVPRRLISDNVITGFECIHAINNKKKGKEGQVAIKLD 128
           I+K + NRFK +L  I S +Q+AF P RLI+DNV+  FE +H +N+K +GKE  ++IKLD
Sbjct: 366 ISKVLANRFKAILPNIISENQSAFTPNRLITDNVLVAFEFMHYLNHKNEGKENYMSIKLD 425

Query: 129 MSKC-------FIREMLIKLGFSERWTKNIMRCVETVTFSVLTNGTPQEEFAPKRGIRQG 188
           MSK        FI+ ++ KLGF E+W   IM CV +V++SVL NG       P RGIRQG
Sbjct: 426 MSKAFDRVEWSFIKGVMEKLGFMEKWIHLIMNCVSSVSYSVLINGEAYGNITPSRGIRQG 485

Query: 189 DPLSPYLFLICAEGFLRLLSREVETHNFKGFRINNHCPTLSHLFFADDSLIFCRENTKEC 248
           DPLSP LFL+CAEG   L+          G  I   CP ++HLFFADDSL+FC+   +EC
Sbjct: 486 DPLSPCLFLLCAEGLSALIHEAARNQQINGISICRGCPLITHLFFADDSLLFCKAKEQEC 545

Query: 249 QVLKEILKNYKEASDQKINLDKSAFMLSKNVKQEEGRIVEEILEKKKKNKGSGQL 297
             L  IL  Y+EAS QKIN DKS+   S N  QE    +  IL   + ++ S  L
Sbjct: 546 HALVSILNRYEEASGQKINTDKSSVFFSPNTSQELRESIFNILGPMQDSRHSKYL 600

BLAST of Lag0027806 vs. NCBI nr
Match: XP_030964220.1 (uncharacterized protein LOC115985421 [Quercus lobata])

HSP 1 Score: 279.6 bits (714), Expect = 3.1e-71
Identity = 135/283 (47.70%), Postives = 183/283 (64.66%), Query Frame = 0

Query: 9   NNYWDIVGEDTLEVCLKILNEDADITPLNKTLIALIPKVKEAKHMHDFRPISLCNVSYKI 68
           +NYWDIVG + + + L +LN +  +T +NKT I+LIPK  +   M +F PISLCN +YKI
Sbjct: 602 HNYWDIVGSNIINMVLSVLNSNLPMTDINKTNISLIPKTNQPTKMTEFHPISLCNTTYKI 661

Query: 69  IAKAIVNRFKKVLDKITSPSQAAFVPRRLISDNVITGFECIHAINNKKKGKEGQVAIKLD 128
           I+K + NR K +L  I S +Q+AF P RLI+DNV+  FE +H +N+K +GKE  ++IKLD
Sbjct: 662 ISKVLANRLKAILPNIISENQSAFTPDRLITDNVLVAFEFMHYLNHKSEGKENYMSIKLD 721

Query: 129 MSKC-------FIREMLIKLGFSERWTKNIMRCVETVTFSVLTNGTPQEEFAPKRGIRQG 188
           MSK        FI+ +++KLGF ++W   +M CV +V++SVL NG      +P RGIRQG
Sbjct: 722 MSKAFDRVEWNFIKGVMVKLGFVDKWIDLVMNCVSSVSYSVLINGEACGNISPSRGIRQG 781

Query: 189 DPLSPYLFLICAEGFLRLLSREVETHNFKGFRINNHCPTLSHLFFADDSLIFCRENTKEC 248
           DPLSP LFL+CAEGF  L+          G  I   CP ++H FFADDSL+FC+   +EC
Sbjct: 782 DPLSPCLFLLCAEGFSALIHEAARNQQINGISICRGCPLITHFFFADDSLLFCKAKVQEC 841

Query: 249 QVLKEILKNYKEASDQKINLDKSAFMLSKNVKQEEGRIVEEIL 285
             L  IL  Y+EAS QKIN DKS+   S N  Q+    + +IL
Sbjct: 842 HALISILNKYEEASGQKINTDKSSVFFSPNTPQDLKESILDIL 884

BLAST of Lag0027806 vs. NCBI nr
Match: XP_023912601.1 (uncharacterized protein LOC112024185 [Quercus suber])

HSP 1 Score: 278.5 bits (711), Expect = 6.9e-71
Identity = 141/295 (47.80%), Postives = 186/295 (63.05%), Query Frame = 0

Query: 9   NNYWDIVGEDTLEVCLKILNEDADITPLNKTLIALIPKVKEAKHMHDFRPISLCNVSYKI 68
           +NYWDIVG     + L +LN +  +T +NKT I+LIPK  +   M +FRPISLCN +YKI
Sbjct: 238 HNYWDIVGPSITNMVLNVLNSNLPMTEINKTNISLIPKTNQPVRMTEFRPISLCNTTYKI 297

Query: 69  IAKAIVNRFKKVLDKITSPSQAAFVPRRLISDNVITGFECIHAINNKKKGKEGQVAIKLD 128
           I+K + NRFK +L  I S +Q+AF P RLI+DNV+  F+ +H +N+K +GKE  ++IKLD
Sbjct: 298 ISKVLANRFKAILPNIISENQSAFTPNRLITDNVLVAFKFMHYLNHKNEGKENYMSIKLD 357

Query: 129 MSKC-------FIREMLIKLGFSERWTKNIMRCVETVTFSVLTNGTPQEEFAPKRGIRQG 188
           MSK        FI+ ++ KLGF E+W   IM CV +V++SVL NG      AP RGIRQG
Sbjct: 358 MSKAFDRVEWSFIKGVMEKLGFMEKWIHLIMNCVSSVSYSVLINGEAFGNIAPSRGIRQG 417

Query: 189 DPLSPYLFLICAEGFLRLLSREVETHNFKGFRINNHCPTLSHLFFADDSLIFCRENTKEC 248
           DPLSP LFL+CA+G   L+          G  I   CP ++HLFFADDSL+FC+   +EC
Sbjct: 418 DPLSPCLFLLCAKGLSALIHEATRNQQINGISICRGCPLITHLFFADDSLLFCKAKEQEC 477

Query: 249 QVLKEILKNYKEASDQKINLDKSAFMLSKNVKQEEGRIVEEILEKKKKNKGSGQL 297
             L  IL  Y+EAS QKIN DKS+   S N  QE    +  IL   + ++ S  L
Sbjct: 478 HALVSILHRYEEASRQKINTDKSSVFFSPNTSQELRESIFNILGPMQDSRHSKYL 532

BLAST of Lag0027806 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 276.9 bits (707), Expect = 2.0e-70
Identity = 143/289 (49.48%), Postives = 183/289 (63.32%), Query Frame = 0

Query: 6   LSINNYWDIVGEDTLEVCLKILNEDADITPLNKTLIALIPKVKEAKHMHDFRPISLCNVS 65
           L    YW +VG  TLE CL  LN   DI   N T IALIPK+K+ + + DFRPISLCNVS
Sbjct: 616 LFYQTYWHVVGPKTLEACLNALNNGDDIKKWNSTYIALIPKIKQPRSISDFRPISLCNVS 675

Query: 66  YKIIAKAIVNRFKKVLDKITSPSQAAFVPRRLISDNVITGFECIHAINNKKKGKEGQVAI 125
           YKII+K+I NR K V+  + S +Q+AFVP R ISDNVI G EC+H IN+ K G  G  A+
Sbjct: 676 YKIISKSITNRLKNVIGLVISDAQSAFVPSRAISDNVIIGHECLHTINSCKSGLIGMAAL 735

Query: 126 KLDMSKCFIRE-------MLIKLGFSERWTKNIMRCVETVTFSVLTNGTPQEEFAPKRGI 185
           KLD+SK F R        ++ K+GF+E W + I++C+ TV FS+  NG+P   F P RGI
Sbjct: 736 KLDLSKAFDRVEWTYLECIMRKMGFNEGWIQAIIQCISTVRFSIHLNGSPGGCFQPSRGI 795

Query: 186 RQGDPLSPYLFLICAEGFLRLLSREVETHNFKGFRINNHCPTLSHLFFADDSLIFCRENT 245
           RQGDPLSPYLFL+CAEG   L++ E  +    G     +  +++HL FADDSLIF R   
Sbjct: 796 RQGDPLSPYLFLLCAEGLSALINHENNSGRLTGIHFEENNTSITHLLFADDSLIFLRSLE 855

Query: 246 KECQVLKEILKNYKEASDQKINLDKSAFMLSKNVKQEEGRIVEEILEKK 288
            EC  L+ +L +Y  AS Q IN  KSA + S NV  E  + ++ IL  K
Sbjct: 856 SECLALRRLLDSYGRASGQCINFSKSALLFSPNVHPERQQYLQCILNVK 904

BLAST of Lag0027806 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.7e-24
Identity = 81/251 (32.27%), Postives = 119/251 (47.41%), Query Frame = 0

Query: 41  IALIPKV-KEAKHMHDFRPISLCNVSYKIIAKAIVNRFKKVLDKITSPSQAAFVPRRLIS 100
           I LIPK  ++     +FRPISL N+  KI+ K + NR ++ + K+    Q  F+P     
Sbjct: 514 IILIPKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQHIKKLIHHDQVGFIPGMQGW 573

Query: 101 DNVITGFECIHAINNKKKGKEGQVAIKLDMSKC-------FIREMLIKLGFSERWTKNIM 160
            N+      I  IN  K   +  V I +D  K        F+ + L KLG    + K I 
Sbjct: 574 FNIRKSINVIQHINRAK--DKNHVIISIDAEKAFDKIQQPFMLKTLNKLGIDGMYLKIIR 633

Query: 161 RCVETVTFSVLTNGTPQEEFAPKRGIRQGDPLSPYLFLICAEGFLRLLSREVETHNFKGF 220
              +  T +++ NG   E F  K G RQG PLSP LF I  E   R + +E E    KG 
Sbjct: 634 AIYDKPTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLEVLARAIRQEKE---IKGI 693

Query: 221 RINNHCPTLSHLFFADDSLIFCRENTKECQVLKEILKNYKEASDQKINLDKSAFMLSKNV 280
           ++      LS   FADD +++        Q L +++ N+ + S  KIN+ KS   L  N 
Sbjct: 694 QLGKEEVKLS--LFADDMIVYLENPIVSAQNLLKLISNFSKVSGYKINVQKSQAFLYNNN 753

Query: 281 KQEEGRIVEEI 284
           +Q E +I+ E+
Sbjct: 754 RQTESQIMGEL 757

BLAST of Lag0027806 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 102.4 bits (254), Expect = 8.9e-21
Identity = 80/250 (32.00%), Postives = 117/250 (46.80%), Query Frame = 0

Query: 41  IALIPK-VKEAKHMHDFRPISLCNVSYKIIAKAIVNRFKKVLDKITSPSQAAFVPRRLIS 100
           I LIPK  K+   + +FRPISL N+  KI+ K + NR ++ +  I  P Q  F+P     
Sbjct: 521 ITLIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQEHIKAIIHPDQVGFIPGMQGW 580

Query: 101 DNVITGFECIHAINNKKKGKEGQVAIKLDMSKCFIR-------EMLIKLGFSERWTKNIM 160
            N+      IH IN  K   +  + I LD  K F +       ++L + G    +   I 
Sbjct: 581 FNIRKSINVIHYINKLK--DKNHMIISLDAEKAFDKIQHPFMIKVLERSGIQGPYLNMIK 640

Query: 161 RCVETVTFSVLTNGTPQEEFAPKRGIRQGDPLSPYLFLICAEGFLRLLSREVETHNFKGF 220
                   ++  NG   E    K G RQG PLSPYLF I  E   R + ++ E    KG 
Sbjct: 641 AIYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLARAIRQQKE---IKGI 700

Query: 221 RINNHCPTLSHLFFADDSLIFCRENTKECQVLKEILKNYKEASDQKINLDKS-AFMLSKN 280
           +I      +S L  ADD +++  +     + L  ++ ++ E    KIN +KS AF+ +KN
Sbjct: 701 QIGKEEVKISLL--ADDMIVYISDPKNSTRELLNLINSFGEVVGYKINSNKSMAFLYTKN 760

Query: 281 VKQEEGRIVE 282
            KQ E  I E
Sbjct: 761 -KQAEKEIRE 762

BLAST of Lag0027806 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 5.8e-20
Identity = 83/264 (31.44%), Postives = 118/264 (44.70%), Query Frame = 0

Query: 41  IALIPKV-KEAKHMHDFRPISLCNVSYKIIAKAIVNRFKKVLDKITSPSQAAFVPRRLIS 100
           I LIPK  K+     ++RPISL N+  KI+ K + NR ++ + KI    Q  F+P     
Sbjct: 513 ITLIPKPGKDPTRKENYRPISLMNIDAKILNKILTNRIQQHIKKIIHHDQVGFIPGSQGW 572

Query: 101 DNVITGFECIHAINNKKKGKEGQVAIKLDMSKC-------FIREMLIKLGFSERWTKNIM 160
            N+      I  I NK K K+  + + +D  K        F+   L K+G    + K I 
Sbjct: 573 FNIRKSINVIQHI-NKLKNKDHMI-LSIDAEKAFDNIQHPFMIRTLKKIGIEGTFLKLIE 632

Query: 161 RCVETVTFSVLTNGTPQEEFAPKRGIRQGDPLSPYLFLICAEGFLRLLSREVETHNFKGF 220
                 T +++ NG   + F  + G RQG PLSP LF I  E  L +  RE +    KG 
Sbjct: 633 AIYSKPTANIILNGVKLKSFPLRSGTRQGCPLSPLLFNIVME-VLAIAIREEKA--IKGI 692

Query: 221 RINNHCPTLSHLFFADDSLIFCRENTKECQVLKEILKNYKEASDQKINLDKSAFMLSKNV 280
            I +    LS   FADD +++          L E++K Y   S  KIN  KS   +  N 
Sbjct: 693 HIGSEEIKLS--LFADDMIVYLENTRDSTTKLLEVIKEYSNVSGYKINTHKSVAFIYTNN 752

Query: 281 KQEEGRIVEEI----LEKKKKNKG 293
            Q E  + + I    + KK K  G
Sbjct: 753 NQAEKTVKDSIPFTVVPKKMKYLG 769

BLAST of Lag0027806 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 2.7e-17
Identity = 74/275 (26.91%), Postives = 122/275 (44.36%), Query Frame = 0

Query: 11  YWDIVGEDTLEVCLKILNEDADITPLNKTLIALIPKVKEAKHMHDFRPISLCNVSYKIIA 70
           +WD +G D   V  +   +        + +++L+PK  + + + ++RP+SL +  YKI+A
Sbjct: 480 FWDTLGPDFHRVLTEAFKKGELPLSCRRAVLSLLPKKGDLRLIKNWRPVSLLSTDYKIVA 539

Query: 71  KAIVNRFKKVLDKITSPSQAAFVPRRLISDNVITGFECIHAINNKKKGKEGQVAIKLDMS 130
           KAI  R K VL ++  P Q+  VP R I DNV    + +H     ++       + LD  
Sbjct: 540 KAISLRLKSVLAEVIHPDQSYTVPGRTIFDNVFLIRDLLHF---ARRTGLSLAFLSLDQE 599

Query: 131 KCFIR-------EMLIKLGFSERWTKNIMRCVETVTFSVLTNGTPQEEFAPKRGIRQGDP 190
           K F R         L    F  ++   +     +    V  N +     A  RG+RQG P
Sbjct: 600 KAFDRVDHQYLIGTLQAYSFGPQFVGYLKTMYASAECLVKINWSLTAPLAFGRGVRQGCP 659

Query: 191 LSPYLFLICAEGFLRLLSREVETHNFKGFRINNHCPTLSHLFFADDSLIFCR-----ENT 250
           LS  L+ +  E FL LL + +      G  +      +    +ADD ++  +     E  
Sbjct: 660 LSGQLYSLAIEPFLCLLRKRL-----TGLVLKEPDMRVVLSAYADDVILVAQDLVDLERA 719

Query: 251 KECQVLKEILKNYKEASDQKINLDKSAFMLSKNVK 274
           +ECQ +      Y  AS  +IN  KS+ +L  ++K
Sbjct: 720 QECQEV------YAAASSARINWSKSSGLLEGSLK 740

BLAST of Lag0027806 vs. ExPASy Swiss-Prot
Match: P92555 (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.8e-13
Identity = 33/68 (48.53%), Postives = 43/68 (63.24%), Query Frame = 0

Query: 163 LTNGTPQEEFAPKRGIRQGDPLSPYLFLICAEGFLRLLSREVETHNFKGFRINNHCPTLS 222
           + NG PQ    P RG+RQGDPLSPYLF++C E    L  R  E     G R++N+ P ++
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 223 HLFFADDS 231
           HL FADD+
Sbjct: 73  HLLFADDT 80

BLAST of Lag0027806 vs. ExPASy TrEMBL
Match: A0A803P996 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 7.2e-74
Identity = 136/267 (50.94%), Postives = 181/267 (67.79%), Query Frame = 0

Query: 12   WDIVGEDTLEVCLKILNEDADITPLNKTLIALIPKVKEAKHMHDFRPISLCNVSYKIIAK 71
            W  VG+   +V L ILN++AD T LNKTLI LIPKVK+ +H+ ++RPISLCNV  K++ K
Sbjct: 876  WTTVGDLVTKVVLSILNDNADPTTLNKTLITLIPKVKKPQHIQEYRPISLCNVISKLVTK 935

Query: 72   AIVNRFKKVLDKITSPSQAAFVPRRLISDNVITGFECIHAINNKKKGKEGQVAIKLDMSK 131
             +V+RFK  L  + S  Q+AF+P RLI+DNV+  FE +HAI NK  G+ G  + KLDMSK
Sbjct: 936  VLVSRFKIALPMVISEEQSAFLPNRLITDNVLVAFELVHAIKNKTAGRNGVASFKLDMSK 995

Query: 132  C-------FIREMLIKLGFSERWTKNIMRCVETVTFSVLTNGTPQEEFAPKRGIRQGDPL 191
                    FI E++ K+GF+ERW   IM C+ T  FS + NG    +  P RG++QG PL
Sbjct: 996  AFDRVEWKFIEEVMRKMGFAERWISLIMSCLTTNNFSFIINGEVMGDLTPSRGLQQGCPL 1055

Query: 192  SPYLFLICAEGFLRLLSREVETHNFKGFRINNHCPTLSHLFFADDSLIFCRENTKECQVL 251
            SPYLFLIC+EGF RLL  E ++HN  GF++  H P ++HLFFADDSL+FC+ N + C  +
Sbjct: 1056 SPYLFLICSEGFSRLLQHEQDSHNLMGFKLTRHAPPITHLFFADDSLLFCQANERSCLAI 1115

Query: 252  KEILKNYKEASDQKINLDKSAFMLSKN 272
            K +L  Y +AS Q++NLDKS    S N
Sbjct: 1116 KRVLDTYHKASGQELNLDKSVMSFSPN 1142

BLAST of Lag0027806 vs. ExPASy TrEMBL
Match: A0A803QC75 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 7.2e-74
Identity = 137/267 (51.31%), Postives = 181/267 (67.79%), Query Frame = 0

Query: 12   WDIVGEDTLEVCLKILNEDADITPLNKTLIALIPKVKEAKHMHDFRPISLCNVSYKIIAK 71
            W IVG+   +V L ILN++AD T LNKTLI LIPKVK+ +H+ ++RPISLCNV  K++ K
Sbjct: 779  WTIVGDLVTKVVLSILNDNADPTALNKTLITLIPKVKKPQHIQEYRPISLCNVISKLVTK 838

Query: 72   AIVNRFKKVLDKITSPSQAAFVPRRLISDNVITGFECIHAINNKKKGKEGQVAIKLDMSK 131
             +V+RFK  L  + S  Q+AF+P RLI+DNV+  FE +HAI NK  G+ G  + KLDMSK
Sbjct: 839  VLVSRFKLALPLVISEEQSAFLPNRLITDNVLVAFELVHAIKNKTAGRNGVASFKLDMSK 898

Query: 132  C-------FIREMLIKLGFSERWTKNIMRCVETVTFSVLTNGTPQEEFAPKRGIRQGDPL 191
                    FI E++ K+GF+ERW   IM C+ T  FS + NG       P RG+RQG PL
Sbjct: 899  AFDRVEWKFIEEVMRKMGFAERWISLIMSCLTTNNFSFIINGEVMGNLTPSRGLRQGCPL 958

Query: 192  SPYLFLICAEGFLRLLSREVETHNFKGFRINNHCPTLSHLFFADDSLIFCRENTKECQVL 251
            SPYLFLIC+EGF RLL  E +++N  GF++  H P ++HLFFADDSL+FC+ N + C  +
Sbjct: 959  SPYLFLICSEGFSRLLQHEQDSNNLMGFKLTRHAPPITHLFFADDSLLFCQANERSCLAI 1018

Query: 252  KEILKNYKEASDQKINLDKSAFMLSKN 272
            K +L  Y +AS Q++NLDKS    S N
Sbjct: 1019 KRVLDTYHKASGQELNLDKSVMSFSPN 1045

BLAST of Lag0027806 vs. ExPASy TrEMBL
Match: A0A803QQL5 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 6.8e-72
Identity = 135/267 (50.56%), Postives = 178/267 (66.67%), Query Frame = 0

Query: 12  WDIVGEDTLEVCLKILNEDADITPLNKTLIALIPKVKEAKHMHDFRPISLCNVSYKIIAK 71
           W +VGE   +V L ILN+ AD T LNKT+I LIPK+K+ + M DFRPISLCNV  K++ K
Sbjct: 85  WSVVGELVTKVVLSILNDGADPTALNKTIITLIPKIKKPQRMKDFRPISLCNVISKLVTK 144

Query: 72  AIVNRFKKVLDKITSPSQAAFVPRRLISDNVITGFECIHAINNKKKGKEGQVAIKLDMSK 131
            +V RFK +L  + S  Q+AF+P RLI+DN++  FE +HAI NK  G+ G    KLDMSK
Sbjct: 145 VLVARFKPILPLVISEEQSAFLPNRLITDNILVAFELVHAIKNKTVGRNGIATFKLDMSK 204

Query: 132 -------CFIREMLIKLGFSERWTKNIMRCVETVTFSVLTNGTPQEEFAPKRGIRQGDPL 191
                  CF+  ++ ++GF ERW   +M C+ T TFS L NG  + E  P RG+RQG PL
Sbjct: 205 AFDRVEWCFLEVVMRQMGFVERWISLVMSCLTTNTFSFLINGEVKGELTPTRGLRQGCPL 264

Query: 192 SPYLFLICAEGFLRLLSREVETHNFKGFRINNHCPTLSHLFFADDSLIFCRENTKECQVL 251
           SPYLFLIC+EGF RLL  E +T + +GF++  + P +SHLFFADDSL+FC+ +   C  +
Sbjct: 265 SPYLFLICSEGFSRLLHHEQQTGHLRGFKLTRNAPPISHLFFADDSLLFCQADDSSCLAI 324

Query: 252 KEILKNYKEASDQKINLDKSAFMLSKN 272
           K +L  Y +AS Q +NLDKS    S N
Sbjct: 325 KRVLDIYHKASGQTLNLDKSVMSFSPN 351

BLAST of Lag0027806 vs. ExPASy TrEMBL
Match: A0A7N2LIH6 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 2.0e-71
Identity = 141/305 (46.23%), Postives = 191/305 (62.62%), Query Frame = 0

Query: 11   YWDIVGEDTLEVCLKILNEDADITPLNKTLIALIPKVKEAKHMHDFRPISLCNVSYKIIA 70
            YWDIVG       L+ LN       +NKT I LIPK K  + + +FRPISLCNV YKII+
Sbjct: 1155 YWDIVGSSVTNCVLQALNSGVMPKDINKTYICLIPKTKNPQKITEFRPISLCNVIYKIIS 1214

Query: 71   KAIVNRFKKVLDKITSPSQAAFVPRRLISDNVITGFECIHAINNKKKGKEGQVAIKLDMS 130
            K + NR KKVL  +   +Q+AFVP R+I+DNVI  FE +H+IN ++KGKEG +AIKLDMS
Sbjct: 1215 KVLANRLKKVLHGVIDEAQSAFVPGRMITDNVIVAFESMHSINQRRKGKEGLMAIKLDMS 1274

Query: 131  K-------CFIREMLIKLGFSERWTKNIMRCVETVTFSVLTNGTPQEEFAPKRGIRQGDP 190
            K        ++  M+ K+GF +RW   IM CV +V+FSVL NG P+  F P RG+RQGDP
Sbjct: 1275 KAYDRVEWAYLESMMKKMGFGDRWISLIMMCVTSVSFSVLINGEPKGSFTPSRGLRQGDP 1334

Query: 191  LSPYLFLICAEGFLRLLSREVETHNFKGFRINNHCPTLSHLFFADDSLIFCRENTKECQV 250
            +SPYLFL+C EG   ++ ++      +G       P +SHLFFADDS+IFCR    EC+ 
Sbjct: 1335 ISPYLFLLCGEGLSAMIKKKEREGLIRGVVAARQAPRISHLFFADDSIIFCRATVDECEQ 1394

Query: 251  LKEILKNYKEASDQKINLDKSAFMLSKNVKQE-----EGRIVEEILEKKKKNKGSGQLFG 304
            + ++L+ Y+E S QK+N DK++   S+N K E     +G    +I++  +K  G   L G
Sbjct: 1395 VAKVLEVYEEESGQKLNRDKTSLFFSRNTKDEMKEFAKGIFGAQIIQHHEKYLGLPPLIG 1454

BLAST of Lag0027806 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 9.8e-71
Identity = 143/289 (49.48%), Postives = 183/289 (63.32%), Query Frame = 0

Query: 6   LSINNYWDIVGEDTLEVCLKILNEDADITPLNKTLIALIPKVKEAKHMHDFRPISLCNVS 65
           L    YW +VG  TLE CL  LN   DI   N T IALIPK+K+ + + DFRPISLCNVS
Sbjct: 616 LFYQTYWHVVGPKTLEACLNALNNGDDIKKWNSTYIALIPKIKQPRSISDFRPISLCNVS 675

Query: 66  YKIIAKAIVNRFKKVLDKITSPSQAAFVPRRLISDNVITGFECIHAINNKKKGKEGQVAI 125
           YKII+K+I NR K V+  + S +Q+AFVP R ISDNVI G EC+H IN+ K G  G  A+
Sbjct: 676 YKIISKSITNRLKNVIGLVISDAQSAFVPSRAISDNVIIGHECLHTINSCKSGLIGMAAL 735

Query: 126 KLDMSKCFIRE-------MLIKLGFSERWTKNIMRCVETVTFSVLTNGTPQEEFAPKRGI 185
           KLD+SK F R        ++ K+GF+E W + I++C+ TV FS+  NG+P   F P RGI
Sbjct: 736 KLDLSKAFDRVEWTYLECIMRKMGFNEGWIQAIIQCISTVRFSIHLNGSPGGCFQPSRGI 795

Query: 186 RQGDPLSPYLFLICAEGFLRLLSREVETHNFKGFRINNHCPTLSHLFFADDSLIFCRENT 245
           RQGDPLSPYLFL+CAEG   L++ E  +    G     +  +++HL FADDSLIF R   
Sbjct: 796 RQGDPLSPYLFLLCAEGLSALINHENNSGRLTGIHFEENNTSITHLLFADDSLIFLRSLE 855

Query: 246 KECQVLKEILKNYKEASDQKINLDKSAFMLSKNVKQEEGRIVEEILEKK 288
            EC  L+ +L +Y  AS Q IN  KSA + S NV  E  + ++ IL  K
Sbjct: 856 SECLALRRLLDSYGRASGQCINFSKSALLFSPNVHPERQQYLQCILNVK 904

BLAST of Lag0027806 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 78.2 bits (191), Expect = 1.3e-14
Identity = 33/68 (48.53%), Postives = 43/68 (63.24%), Query Frame = 0

Query: 163 LTNGTPQEEFAPKRGIRQGDPLSPYLFLICAEGFLRLLSREVETHNFKGFRINNHCPTLS 222
           + NG PQ    P RG+RQGDPLSPYLF++C E    L  R  E     G R++N+ P ++
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 223 HLFFADDS 231
           HL FADD+
Sbjct: 73  HLLFADDT 80

BLAST of Lag0027806 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 59.3 bits (142), Expect = 6.2e-09
Identity = 29/88 (32.95%), Postives = 51/88 (57.95%), Query Frame = 0

Query: 73  IVNRFKKVLDKITSPSQAAFVPRRLISDNVITGFECIHAINNKKKGKEGQVAIKLDMSKC 132
           +V R K ++  +  P+QA+F+P R+ +DN++   E +H++  +KKG +G + +KLD+ K 
Sbjct: 1   MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSM-RRKKGVKGWMLLKLDLEKA 60

Query: 133 -------FIREMLIKLGFSERWTKNIMR 154
                  ++ + LI  GF E W   I R
Sbjct: 61  YDRIRWDYLEDTLISAGFPEVWLPEIAR 87

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023881891.14.8e-7248.72uncharacterized protein LOC111994244 [Quercus suber][more]
XP_023901742.18.2e-7248.14uncharacterized protein LOC112013579 [Quercus suber][more]
XP_030964220.13.1e-7147.70uncharacterized protein LOC115985421 [Quercus lobata][more]
XP_023912601.16.9e-7147.80uncharacterized protein LOC112024185 [Quercus suber][more]
XP_022158377.12.0e-7049.48uncharacterized protein LOC111024874 [Momordica charantia][more]
Match NameE-valueIdentityDescription
O003701.7e-2432.27LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1[more]
P113698.9e-2132.00LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE... [more]
P085485.8e-2031.44LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1[more]
P143812.7e-1726.91Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV... [more]
P925551.8e-1348.53Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A803P9967.2e-7450.94Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803QC757.2e-7451.31Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803QQL56.8e-7250.56Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A7N2LIH62.0e-7146.23Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1[more]
A0A6J1DX309.8e-7149.48uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
Match NameE-valueIdentityDescription
ATMG01250.11.3e-1448.53RNA-directed DNA polymerase (reverse transcriptase) [more]
AT4G20520.16.2e-0932.95RNA binding;RNA-directed DNA polymerases [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 244..264
NoneNo IPR availablePANTHERPTHR19446:SF440SUBFAMILY NOT NAMEDcoord: 8..272
NoneNo IPR availablePANTHERPTHR19446REVERSE TRANSCRIPTASEScoord: 8..272
NoneNo IPR availableCDDcd01650RT_nLTR_likecoord: 38..283
e-value: 1.55392E-45
score: 151.675
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 46..275
e-value: 9.0E-37
score: 126.7
IPR000477Reverse transcriptase domainPROSITEPS50878RT_POLcoord: 25..301
score: 8.568132
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 21..271

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0027806.1Lag0027806.1mRNA