Lag0027739 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0027739
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionNHL domain-containing protein
Locationchr8: 4317701 .. 4321110 (+)
RNA-Seq ExpressionLag0027739
SyntenyLag0027739
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAGGCGTTGGGTCCTTTTGGCAGCCATTTTCGGGGTTCTCTTTTGTGGGTTTTCTTCAGTTTCTGCCACACCCACAAGTACGACCTACTTCTCTAACCTCGCCTCTTGTTTTTCTCTTCTCGTACTTCATCTCTGTTTAGGCGTTTTCGTTTCATTTGCAGAAATTCTTGCTGGGGTTTTGTCCAAGGCGGTTTCGGCTCTTGTGAGTTGGATGTGGGCGATCAGCTCGGCCTCTAATACTGGTTTGATTCGTTTCCTTTTCCCTAATTTTCAGCGTTTTCTGTGTGTTTGATCGATTTGTGGGGTTTTGTTTTTGTTGATTTTTCAGCCGTTTCTACCCGGTCAATGATTAAATTTGAAGGGGGATATGTTGTTGAGACTGTGTTTGATGGTAGTAAGCTGGGGATTGAGCCTTACTCTGTTGGGGTATCTCCTAGTGGAGAGCTTTTGATTTTGGATGCTGAAAATAGTAATGTTCACAAGATCTCTATGCCATTTTCTCAATGTAAGCCTCTTTCTTTATTGATTTATCTGCTCAATTGTGTAGGTTAGATTATCTTTCTTGCCTTGGAACATTTTTCTTGTTAGAATATAGTGTTATGAATGTGGGGTTGAGAATTCGATTCTTTGGCTGGTAAGGAAGATTATTAATGCCTTAATCGTTGAGGTAAACTCAAATTGGATTTGGCACTAGACATGATACTTTGCTTTTTTAACGTTTTTGTCATTTCTTTTTCAATTTCCATCTTTATACTTTCAGTTTTATGAACGTCTTAAATAGTAACAGAGCGTTCACATGAGATGACATTGGATGTATGCAAACTTATCGTAAATCTGTTGTGGTACATTAGCTTTTTGCCATTGGAATTGCATGAACTAAAATGAAATGGGTACCATGACCAAAAGATTAAAAAGTAGCAAATATAGAAATTAACATTGTAAATTTAGTATTCTAATAGCTATGGAAATCAGTCCGTTTGCTTGTTCTTTGTCTAGCCTTGTGGTAGCTGCTAGACTGCTCGTACTTTGCTTCAAACTTTGGACATCTTTTCTTTTCTTTTCTCTTTTATGATTTTTTTTTTCAACAGATTCTTTGCCTTCTTTAAGTATTAGTTGATTCCAAATGTAGATCATAGTACTTTTACTTTGCTTACAAGCTTTTGTTTAGTAGAAGACTCTACTTTTTATTGTAAAACTATCCTTTTCAAAAGTTCTGCAGTTACATTAAAGATCTATATCTCTTATGTTAGACCAATATTGAGCTTCATTAGCTCTTAGTCCTTTAGCTTTACTTACCATGATTCAAGGCTTTGACGTACAAAATCAATCAATGGAAGTGTACTTTTCCTGTATCTAATAAGTTTGAGCTTCCTGAAGAAGACATGTATTGTCTTATTTGGTTGTTAGACCACCTCATTTCACTTTACTTTTCAAAGATATTAGTTTTGCAGTATCACGAGCATTTGGACCCACTTACATGTACTAAATAATTTTAGTAGCCAATAGCATGACATTAAAATATTTGATATTTAAAAGTTCGTGGAAGGCGATCAAGGTAACAAACTGATGAGTCATATAGGTAATTAACTAGCAGTCTTGCAGTGTTTAATACTGAATTTACTCGAGTAGAATTCCTCATTGAATGATTTTAAGGAGTCAAAACTAGCTTAATATATTTGTACAAAAATCGCGTGACAAGGCATATTTTATACTCTTTTAAAAAATATTGTTTTTTTTTTTCCATATTGATGTAAATAGCATATTTTTTTACACCTAAGACCAATAAATGGTTTTTATCCTATAATACTTCCACTTTTCATCCTACCAAGTGATATGCCATTCAGTTATAACTCATGGTTTAAATTTGAAAATTTACATCATGTCACTCAGTTTCACTCTGATTATTCGATAACTCGGGCGGGGTAAAATAGAATTGCTCTTCTAATTTGATGAACTATTCGTTACTCTAGTTTCTTCTTTTTCTGAAAGAAAGAACCATATACAGTTTGCAGGCCCAAACTGTTAGCAGGATCATCTGAAGGATACTCTGGACATGTAGATGGAAAGCTTAGGGATGCAAGAATGAGCCATCCAAGAGGGCTAACAGTGGATCAAAGAGGAAACATTTACATTGCAGATACAAAGAACAAGGCTATAAGAAAAATCAGTGATGCCGGTAATTTCCTAAATCGGTTCGAGAGCATTTCACTATTGTCGACTTCTTGTTCATTAAGTTTACTAGGAATCTCCATTCAGTTACATTTTGTAGCATTCAACTTCTTGGTTTTGGGATCCGTTAATTGTTTATAAGTCGTTTATAGTTTTTATGCAGGAGTTACGACTATTGCTGGTGGTAAATGGAGGAAAAGTGGCCACGTTGATGGTTCAAGTGAAGAATCAAAGTTCTCAAATGATTTTGATGTGGTTTATGTAGGAAGTAGCTGTTCACTTCTGGTAGTCGACAGAGGAAACCAGGCAATTCGAGAGATTCAACTCCGTGCCGATGATTGTACCAACGAATATGATGGTAGCTTCCTCCTAGGTACATTGTAGCCAGAACAAATCAACATTAGGTGCCATCTTTCTCTCCTCTTCAGCAAGCTATTAACTATATTCTGTTAATTGTAGGGATGGCTCTGCTGATTGCAGCTATGCTTTCAGGATACATGTTGGCACGTTTTCAATTTCGAGTGTTGGCGACGTTCTCTTCGAAAAGTGTAACGTCATCTCAAATCTTACAAAACAAACAATAGCTTTTAGTCTTATTGTTAATGTAGCAATGGAGTGAAATCCATCAGCATTGACATTTCCCAGTAGTTTTACTGATGTTTCTGCTTTGTTGCTAAGAGGAGGGAGTGATTGACCAGGCTATTCAACATAGAATCAACTTGCAGTTACAGGTTTCTACCTTTCCTATACTTCTTTTTCCCTGAAAAATTTTCCTGTGTTTTGCAGGATTCAAGGACTGATGCAAGGAACGTTACATCGATTCCTCCATTTCGGAGACCCGAGAAATCAGTCAGGCATCCATTGATTCCTAGTGAAGAGGAAGAAGATGAACCTGGAGAGAATATCATTTGTTCCCTGGGCAAGCTTTTCCTCAACACTGGTTCATCCGCGGCCGAAATCTTTGTGGCATTGCTTCTCGGAGCTAGAAGAAAGCCATCCCACCCCCAAGTCCAGCAGCACTATCGAGTAAACAAACACGCGCAGTCACGGTTTGGTATACAGGGAAGCATTGCAGTCCCACAGGGGCGCGAACCACTGCATACAATGGCGAGCAAACCTTACTCTTATTCGGCAGCACAACTGGAAAATGTTCACCATTACAGGCGCATGTGGGGCGACAATGGAGGACGTGATGTAAAATCATCCTTATTGCATGTACTCATAATGAAACCCTAA

mRNA sequence

ATGGCGAGGCGTTGGGTCCTTTTGGCAGCCATTTTCGGGGTTCTCTTTTGTGGGTTTTCTTCAGTTTCTGCCACACCCACAAAAATTCTTGCTGGGGTTTTGTCCAAGGCGGTTTCGGCTCTTGTGAGTTGGATGTGGGCGATCAGCTCGGCCTCTAATACTGCCGTTTCTACCCGGTCAATGATTAAATTTGAAGGGGGATATGTTGTTGAGACTGTGTTTGATGGTAGTAAGCTGGGGATTGAGCCTTACTCTGTTGGGGTATCTCCTAGTGGAGAGCTTTTGATTTTGGATGCTGAAAATAGTAATGTTCACAAGATCTCTATGCCATTTTCTCAATTTTGCAGGCCCAAACTGTTAGCAGGATCATCTGAAGGATACTCTGGACATGTAGATGGAAAGCTTAGGGATGCAAGAATGAGCCATCCAAGAGGGCTAACAGTGGATCAAAGAGGAAACATTTACATTGCAGATACAAAGAACAAGGCTATAAGAAAAATCAGTGATGCCGGAGTTACGACTATTGCTGGTGGTAAATGGAGGAAAAGTGGCCACGTTGATGGTTCAAGTGAAGAATCAAAGTTCTCAAATGATTTTGATGTGGTTTATGTAGGAAGTAGCTGTTCACTTCTGGTAGTCGACAGAGGAAACCAGGCAATTCGAGAGATTCAACTCCGTGCCGATGATTGTACCAACGAATATGATGGTAGCTTCCTCCTAGGGATGGCTCTGCTGATTGCAGCTATGCTTTCAGGATACATGTTGGCACGTTTTCAATTTCGAGTGTTGGCGACGTTCTCTTCGAAAAGTGATTCAAGGACTGATGCAAGGAACGTTACATCGATTCCTCCATTTCGGAGACCCGAGAAATCAGTCAGGCATCCATTGATTCCTAGTGAAGAGGAAGAAGATGAACCTGGAGAGAATATCATTTGTTCCCTGGGCAAGCTTTTCCTCAACACTGGTTCATCCGCGGCCGAAATCTTTGTGGCATTGCTTCTCGGAGCTAGAAGAAAGCCATCCCACCCCCAAGTCCAGCAGCACTATCGAGTAAACAAACACGCGCAGTCACGGTTTGGTATACAGGGAAGCATTGCAGTCCCACAGGGGCGCGAACCACTGCATACAATGGCGAGCAAACCTTACTCTTATTCGGCAGCACAACTGGAAAATGTTCACCATTACAGGCGCATGTGGGGCGACAATGGAGGACGTGATGTAAAATCATCCTTATTGCATGTACTCATAATGAAACCCTAA

Coding sequence (CDS)

ATGGCGAGGCGTTGGGTCCTTTTGGCAGCCATTTTCGGGGTTCTCTTTTGTGGGTTTTCTTCAGTTTCTGCCACACCCACAAAAATTCTTGCTGGGGTTTTGTCCAAGGCGGTTTCGGCTCTTGTGAGTTGGATGTGGGCGATCAGCTCGGCCTCTAATACTGCCGTTTCTACCCGGTCAATGATTAAATTTGAAGGGGGATATGTTGTTGAGACTGTGTTTGATGGTAGTAAGCTGGGGATTGAGCCTTACTCTGTTGGGGTATCTCCTAGTGGAGAGCTTTTGATTTTGGATGCTGAAAATAGTAATGTTCACAAGATCTCTATGCCATTTTCTCAATTTTGCAGGCCCAAACTGTTAGCAGGATCATCTGAAGGATACTCTGGACATGTAGATGGAAAGCTTAGGGATGCAAGAATGAGCCATCCAAGAGGGCTAACAGTGGATCAAAGAGGAAACATTTACATTGCAGATACAAAGAACAAGGCTATAAGAAAAATCAGTGATGCCGGAGTTACGACTATTGCTGGTGGTAAATGGAGGAAAAGTGGCCACGTTGATGGTTCAAGTGAAGAATCAAAGTTCTCAAATGATTTTGATGTGGTTTATGTAGGAAGTAGCTGTTCACTTCTGGTAGTCGACAGAGGAAACCAGGCAATTCGAGAGATTCAACTCCGTGCCGATGATTGTACCAACGAATATGATGGTAGCTTCCTCCTAGGGATGGCTCTGCTGATTGCAGCTATGCTTTCAGGATACATGTTGGCACGTTTTCAATTTCGAGTGTTGGCGACGTTCTCTTCGAAAAGTGATTCAAGGACTGATGCAAGGAACGTTACATCGATTCCTCCATTTCGGAGACCCGAGAAATCAGTCAGGCATCCATTGATTCCTAGTGAAGAGGAAGAAGATGAACCTGGAGAGAATATCATTTGTTCCCTGGGCAAGCTTTTCCTCAACACTGGTTCATCCGCGGCCGAAATCTTTGTGGCATTGCTTCTCGGAGCTAGAAGAAAGCCATCCCACCCCCAAGTCCAGCAGCACTATCGAGTAAACAAACACGCGCAGTCACGGTTTGGTATACAGGGAAGCATTGCAGTCCCACAGGGGCGCGAACCACTGCATACAATGGCGAGCAAACCTTACTCTTATTCGGCAGCACAACTGGAAAATGTTCACCATTACAGGCGCATGTGGGGCGACAATGGAGGACGTGATGTAAAATCATCCTTATTGCATGTACTCATAATGAAACCCTAA

Protein sequence

MARRWVLLAAIFGVLFCGFSSVSATPTKILAGVLSKAVSALVSWMWAISSASNTAVSTRSMIKFEGGYVVETVFDGSKLGIEPYSVGVSPSGELLILDAENSNVHKISMPFSQFCRPKLLAGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKWRKSGHVDGSSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRADDCTNEYDGSFLLGMALLIAAMLSGYMLARFQFRVLATFSSKSDSRTDARNVTSIPPFRRPEKSVRHPLIPSEEEEDEPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKPSHPQVQQHYRVNKHAQSRFGIQGSIAVPQGREPLHTMASKPYSYSAAQLENVHHYRRMWGDNGGRDVKSSLLHVLIMKP
Homology
BLAST of Lag0027739 vs. NCBI nr
Match: XP_038903583.1 (uncharacterized protein LOC120090138 [Benincasa hispida])

HSP 1 Score: 667.2 bits (1720), Expect = 9.6e-188
Identity = 344/411 (83.70%), Postives = 366/411 (89.05%), Query Frame = 0

Query: 1   MARRWVLLAAIFGVLFCGFSSVSATPTKILAGVLSKAVSALVSWMWAISSASNTAVSTRS 60
           MARRW LLAAIF VLFCGFSSVSATPTKI+AGVLSKAVSALVSW+W+I+SASNT VSTRS
Sbjct: 1   MARRWALLAAIFAVLFCGFSSVSATPTKIVAGVLSKAVSALVSWIWSITSASNTVVSTRS 60

Query: 61  MIKFEGGYVVETVFDGSKLGIEPYSVGVSPSGELLILDAENSNVHKISMPFSQFCRPKLL 120
           MIKFE GYVVET+FDGSKLGIEPYSV VSPSGELLILDAENSNVHKISMPFSQFCRPKL 
Sbjct: 61  MIKFERGYVVETLFDGSKLGIEPYSVAVSPSGELLILDAENSNVHKISMPFSQFCRPKLF 120

Query: 121 AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180
           AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW
Sbjct: 121 AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180

Query: 181 RKSGHVDGSSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRADDCTNEYDGSFLL 240
           RKSGH+DG SEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQL ADDCT EYD SFLL
Sbjct: 181 RKSGHIDGPSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLHADDCT-EYDRSFLL 240

Query: 241 GMALLIAAMLSGYMLARFQFRVLATFSSKSDSRTDARNVTSIPPFRRPEKSVRHPLIPSE 300
           GMALL AA+L GYMLAR QFRVLA FSSK+D R D+RN+TSIPP+ R EKSVR+PLIPSE
Sbjct: 241 GMALLTAAVLLGYMLARLQFRVLAMFSSKNDLRVDSRNITSIPPYGRVEKSVRYPLIPSE 300

Query: 301 EEED-EPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKPSHPQVQQHYRVNKHAQSRF 360
           EEED +P EN+ICSLGKLFLNTGSSAAEIFVALLLGARRK S    QQHY+V +H  SRF
Sbjct: 301 EEEDNQPEENVICSLGKLFLNTGSSAAEIFVALLLGARRKASDSHGQQHYQVKEHVPSRF 360

Query: 361 GIQGSIAVPQGREPLHTMASKPYSYSAAQLENVHHYRRMWGDNGGRDVKSS 411
            +Q + A   G EPL TM  KPY+YS  QLENV HYR  WGD+GGR+ + S
Sbjct: 361 SVQENFAALHGCEPLETMTRKPYTYSTTQLENVQHYRHNWGDDGGREEQPS 410

BLAST of Lag0027739 vs. NCBI nr
Match: XP_004141183.2 (uncharacterized protein LOC101216407 [Cucumis sativus] >KGN59643.1 hypothetical protein Csa_001016 [Cucumis sativus])

HSP 1 Score: 665.2 bits (1715), Expect = 3.6e-187
Identity = 341/407 (83.78%), Postives = 366/407 (89.93%), Query Frame = 0

Query: 1   MARRWVLLAAIFGVLFCGFSSVSATPTKILAGVLSKAVSALVSWMWAISSASNTAVSTRS 60
           MARRWV+LA IF VLFCGFSSVSA PTKI+AGVLSKAVSALVSW+WAI SASNT VSTRS
Sbjct: 1   MARRWVVLAVIFAVLFCGFSSVSALPTKIVAGVLSKAVSALVSWIWAIRSASNTGVSTRS 60

Query: 61  MIKFEGGYVVETVFDGSKLGIEPYSVGVSPSGELLILDAENSNVHKISMPFSQFCRPKLL 120
           MIKFE GYVVET+ DGSK+GIEPYSVGVSPSGELLILDAENSNVHKISMP SQFCRPKL 
Sbjct: 61  MIKFERGYVVETLLDGSKMGIEPYSVGVSPSGELLILDAENSNVHKISMPVSQFCRPKLF 120

Query: 121 AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180
           AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW
Sbjct: 121 AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180

Query: 181 RKSGHVDGSSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRADDCTNEYDGSFLL 240
           RKSGH+DG SEESKFSNDFD+VYVGSSCSLLVVDRGNQAIREIQLRA+DCT EYDGSFLL
Sbjct: 181 RKSGHIDGPSEESKFSNDFDLVYVGSSCSLLVVDRGNQAIREIQLRAEDCT-EYDGSFLL 240

Query: 241 GMALLIAAMLSGYMLARFQFRVLATFSSKSDSRTDARNVTSIPPFRRPEKSVRHPLIPSE 300
           G+ALL AAML GYMLARFQFRVLATFSSK+DSR D RN+ SIPP+ R EKS+R PLIPSE
Sbjct: 241 GIALLTAAMLLGYMLARFQFRVLATFSSKNDSRVDLRNIPSIPPYGRVEKSIRRPLIPSE 300

Query: 301 EEED-EPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKPSHPQVQQHYRVNKHAQSRF 360
           E ED +P ENIICSLGKLFLNTGSSAAEIFVALLLGARRK S    ++HY+VNKHA SRF
Sbjct: 301 EAEDNQPEENIICSLGKLFLNTGSSAAEIFVALLLGARRKASDSHSREHYQVNKHAPSRF 360

Query: 361 GIQGSIAVPQGREPLHTMASKPYSYSAAQLENVHHYRRMWGDNGGRD 407
           G+Q + A   GREPL TM  KPYS S  ++ENV  Y+R+WG+NGGR+
Sbjct: 361 GVQENFAASYGREPLETMTRKPYSCSTTRVENVQRYKRIWGNNGGRE 406

BLAST of Lag0027739 vs. NCBI nr
Match: XP_008443491.1 (PREDICTED: uncharacterized protein LOC103487068 [Cucumis melo] >KAA0053690.1 NHL domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 661.8 bits (1706), Expect = 4.0e-186
Identity = 340/407 (83.54%), Postives = 365/407 (89.68%), Query Frame = 0

Query: 1   MARRWVLLAAIFGVLFCGFSSVSATPTKILAGVLSKAVSALVSWMWAISSASNTAVSTRS 60
           MARRWV+LA IF VLFCGFSSVSA PTKI+AG LSKAVSALV+W+W I+SASNT VSTRS
Sbjct: 1   MARRWVVLAVIFAVLFCGFSSVSAVPTKIVAGALSKAVSALVNWIWTITSASNTVVSTRS 60

Query: 61  MIKFEGGYVVETVFDGSKLGIEPYSVGVSPSGELLILDAENSNVHKISMPFSQFCRPKLL 120
           MIKFE GYVVET+ DGSK+GIEPYSVGVSPSGELLILDAENSNVHKISMP SQFCRPKL 
Sbjct: 61  MIKFERGYVVETLLDGSKMGIEPYSVGVSPSGELLILDAENSNVHKISMPVSQFCRPKLF 120

Query: 121 AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180
           AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW
Sbjct: 121 AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180

Query: 181 RKSGHVDGSSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRADDCTNEYDGSFLL 240
           RKSGH+DG SE+SKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRA+DCT EYDGSFLL
Sbjct: 181 RKSGHLDGPSEDSKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRAEDCT-EYDGSFLL 240

Query: 241 GMALLIAAMLSGYMLARFQFRVLATFSSKSDSRTDARNVTSIPPFRRPEKSVRHPLIPSE 300
           GMALL AAML GYMLARFQFRVLATFSSK+DSR  +RN+ SIPP+ R EKSVR PLIPSE
Sbjct: 241 GMALLTAAMLLGYMLARFQFRVLATFSSKNDSRVGSRNIPSIPPYGRVEKSVRRPLIPSE 300

Query: 301 EEED-EPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKPSHPQVQQHYRVNKHAQSRF 360
           EEED +P ENIICSLGKLFLNTGSSAAEIFVALLLGARRK S    ++HY+VNKHA SRF
Sbjct: 301 EEEDNQPEENIICSLGKLFLNTGSSAAEIFVALLLGARRKASDSHSREHYQVNKHAPSRF 360

Query: 361 GIQGSIAVPQGREPLHTMASKPYSYSAAQLENVHHYRRMWGDNGGRD 407
           G+Q + A   GRE L TM  KPYS S  +LE+V  Y+R+WGDNGGR+
Sbjct: 361 GVQENFAASYGRETLETMTRKPYSCSTTRLESVQRYKRIWGDNGGRE 406

BLAST of Lag0027739 vs. NCBI nr
Match: KAG6581010.1 (hypothetical protein SDJN03_21012, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 649.4 bits (1674), Expect = 2.1e-182
Identity = 336/404 (83.17%), Postives = 363/404 (89.85%), Query Frame = 0

Query: 1   MARRWVLLAAIFGVLFCGFSSVSATPTKILAGVLSKAVSALVSWMWAISSASNTAVSTRS 60
           MA+RWVLLAAIF VL CGFSSVSAT T  +AGVLSKAVSALV+W+ AI+SAS+T VSTRS
Sbjct: 1   MAKRWVLLAAIFAVLVCGFSSVSATHTNFVAGVLSKAVSALVNWISAITSASSTVVSTRS 60

Query: 61  MIKFEGGYVVETVFDGSKLGIEPYSVGVSPSGELLILDAENSNVHKISMPFSQFCRPKLL 120
           MI FE GYVVETVFDGSKLGIEPYSVGVSPSGELLILD+ENSN+HKISMP SQFCRP+L 
Sbjct: 61  MITFEKGYVVETVFDGSKLGIEPYSVGVSPSGELLILDSENSNIHKISMPSSQFCRPQLF 120

Query: 121 AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180
           AGSSEGYSGHVDGK RDARMSHPRGL VDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW
Sbjct: 121 AGSSEGYSGHVDGKPRDARMSHPRGLAVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180

Query: 181 RKSGHVDGSSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRADDCTNEYDGSFLL 240
           RKSGH+DG SEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREI+LRADDCT+E+DGSFLL
Sbjct: 181 RKSGHIDGPSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIRLRADDCTDEFDGSFLL 240

Query: 241 GMALLIAAMLSGYMLARFQFRVLATFSSKSDSRTDARNVTSIPPFRRPEKSVRHPLIPSE 300
           GMALL AAML GYMLAR QF VLA FSSK+DSR D+R+V+SIPP+RR EKSVRHPLIPSE
Sbjct: 241 GMALLAAAMLLGYMLARLQFWVLAAFSSKNDSRADSRDVSSIPPYRRAEKSVRHPLIPSE 300

Query: 301 EEED-EPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKPSHPQVQQHYRVNKHAQSRF 360
           EEED +PGENIICSLGKLFLNTGSSAAEIFVALLLGARRK + P VQQHY+VNKH Q+  
Sbjct: 301 EEEDNQPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKAADPHVQQHYQVNKHGQAGL 360

Query: 361 GIQGSIAVPQGREPLHTMASKPYSYSAAQLENVHHYRRMWGDNG 404
           G+Q S A+P GREPLHTM    YSYSA + ENV  YRR+ G +G
Sbjct: 361 GMQESFAIPCGREPLHTMTGIHYSYSATRPENVQRYRRIRGRDG 404

BLAST of Lag0027739 vs. NCBI nr
Match: XP_022934912.1 (uncharacterized protein LOC111441944 [Cucurbita moschata])

HSP 1 Score: 639.0 bits (1647), Expect = 2.8e-179
Identity = 330/399 (82.71%), Postives = 360/399 (90.23%), Query Frame = 0

Query: 1   MARRWVLLAAIFGVLFCGFSSVSATPTKILAGVLSKAVSALVSWMWAISSASNTAVSTRS 60
           MA+RWVLLAAIF VL CGFSSVSAT T  +AGVLSKAVSALV+W+ AI+SAS+T VSTRS
Sbjct: 1   MAKRWVLLAAIFAVLVCGFSSVSATHTNFVAGVLSKAVSALVNWISAITSASSTVVSTRS 60

Query: 61  MIKFEGGYVVETVFDGSKLGIEPYSVGVSPSGELLILDAENSNVHKISMPFSQFCRPKLL 120
           MI FE GYVVET+FDGSKLGIEPYSVGVSPSGELLILD+ENSN+HKISMP SQFCRP+L 
Sbjct: 61  MITFEKGYVVETMFDGSKLGIEPYSVGVSPSGELLILDSENSNIHKISMPSSQFCRPQLF 120

Query: 121 AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180
           AGSSEGYSGHVDGK RDARMSHPRGL VDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW
Sbjct: 121 AGSSEGYSGHVDGKPRDARMSHPRGLAVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180

Query: 181 RKSGHVDGSSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRADDCTNEYDGSFLL 240
           RKSGH+DG SEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREI+LRA+DCT+E+DGSFLL
Sbjct: 181 RKSGHIDGPSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIRLRAEDCTDEFDGSFLL 240

Query: 241 GMALLIAAMLSGYMLARFQFRVLATFSSKSDSRTDARNVTSIPPFRRPEKSVRHPLIPSE 300
           GMALL AAML GYMLAR QF VLA FSSK+DSR D+R+V+SIPP+RR EKS+RHPLIPSE
Sbjct: 241 GMALLTAAMLLGYMLARLQFWVLAAFSSKNDSRADSRDVSSIPPYRRVEKSMRHPLIPSE 300

Query: 301 EEED-EPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKPSHPQVQQHYRVNKHAQSRF 360
           EEED +PGENIICSLGKLFLNTGSSAAEIFVALLLGARRK ++  VQQHY+VNKH Q+  
Sbjct: 301 EEEDNQPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKAANLHVQQHYQVNKHGQAGL 360

Query: 361 GIQGSIAVPQGREPLHTMASKPYSYSAAQLENVHHYRRM 399
           G+Q S A+P GREPLHTM    YSYSA + ENV  YRR+
Sbjct: 361 GMQESFAIPCGREPLHTMTGIHYSYSATRPENVQRYRRI 399

BLAST of Lag0027739 vs. ExPASy Swiss-Prot
Match: Q8VZ10 (Protein SUPPRESSOR OF QUENCHING 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SOQ1 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 5.6e-05
Identity = 43/153 (28.10%), Postives = 69/153 (45.10%), Query Frame = 0

Query: 82  EPYSVGVSPS-GELLILDAENSNVHKISMPFSQFCRPKLLAGSSEGYS------GHVDGK 141
           +P  + + P   E  I D+E+S++  + +   Q    +LLAG    +S      G  DG 
Sbjct: 740 QPSGISLGPDLKEAYIADSESSSIRALDL---QTGGSRLLAGGDPYFSENLFKFGDNDGV 799

Query: 142 LRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDA--GVTTIAGGKWRKSGHVDGSSEE 201
             +  + HP G+     G IY+ D+ N  I+K+      V T+AG    K+G  DG  + 
Sbjct: 800 GAEVLLQHPLGVLCANDGQIYLTDSYNHKIKKLDPVTKRVVTLAG--TGKAGFKDGKVKG 859

Query: 202 SKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQL 226
           ++ S    +  +  +  L V D  N  IR I L
Sbjct: 860 AQLSEPAGLA-ITENGRLFVADTNNSLIRYIDL 886

BLAST of Lag0027739 vs. ExPASy Swiss-Prot
Match: A4IF69 (NHL repeat-containing protein 2 OS=Bos taurus OX=9913 GN=NHLRC2 PE=2 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 9.5e-05
Identity = 42/140 (30.00%), Postives = 69/140 (49.29%), Query Frame = 0

Query: 94  LLILDAENSNVHKISMPFSQFCRPKLLAGSSEGYS----GHVDGKLRDARMSHPRGLTVD 153
           L + D+E+S V  +S+         L+ G  +  +    G VDG   +AR+ HP G+T D
Sbjct: 426 LFVADSESSTVRTVSL--KDGAVKHLVGGERDPMNLFAFGDVDGVGINARLQHPLGVTWD 485

Query: 154 QRGN-IYIADTKNKAIRKI--SDAGVTTIAGGKWRKSGHVDGSSEESKFSNDFDVVYVGS 213
           Q+ N +Y+AD+ N  I+ +       TT+AG     + ++ GSS      N+   + +G 
Sbjct: 486 QKRNLLYVADSYNHKIKVVDPKTKNCTTLAG--TGNASNMIGSSFTDSTFNEPGGLCIGE 545

Query: 214 SCSLL-VVDRGNQAIREIQL 226
           +  LL V D  N  I+ + L
Sbjct: 546 NGQLLYVADTNNHQIKVLDL 561

BLAST of Lag0027739 vs. ExPASy TrEMBL
Match: A0A0A0LCX9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G835800 PE=4 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 1.8e-187
Identity = 341/407 (83.78%), Postives = 366/407 (89.93%), Query Frame = 0

Query: 1   MARRWVLLAAIFGVLFCGFSSVSATPTKILAGVLSKAVSALVSWMWAISSASNTAVSTRS 60
           MARRWV+LA IF VLFCGFSSVSA PTKI+AGVLSKAVSALVSW+WAI SASNT VSTRS
Sbjct: 1   MARRWVVLAVIFAVLFCGFSSVSALPTKIVAGVLSKAVSALVSWIWAIRSASNTGVSTRS 60

Query: 61  MIKFEGGYVVETVFDGSKLGIEPYSVGVSPSGELLILDAENSNVHKISMPFSQFCRPKLL 120
           MIKFE GYVVET+ DGSK+GIEPYSVGVSPSGELLILDAENSNVHKISMP SQFCRPKL 
Sbjct: 61  MIKFERGYVVETLLDGSKMGIEPYSVGVSPSGELLILDAENSNVHKISMPVSQFCRPKLF 120

Query: 121 AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180
           AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW
Sbjct: 121 AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180

Query: 181 RKSGHVDGSSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRADDCTNEYDGSFLL 240
           RKSGH+DG SEESKFSNDFD+VYVGSSCSLLVVDRGNQAIREIQLRA+DCT EYDGSFLL
Sbjct: 181 RKSGHIDGPSEESKFSNDFDLVYVGSSCSLLVVDRGNQAIREIQLRAEDCT-EYDGSFLL 240

Query: 241 GMALLIAAMLSGYMLARFQFRVLATFSSKSDSRTDARNVTSIPPFRRPEKSVRHPLIPSE 300
           G+ALL AAML GYMLARFQFRVLATFSSK+DSR D RN+ SIPP+ R EKS+R PLIPSE
Sbjct: 241 GIALLTAAMLLGYMLARFQFRVLATFSSKNDSRVDLRNIPSIPPYGRVEKSIRRPLIPSE 300

Query: 301 EEED-EPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKPSHPQVQQHYRVNKHAQSRF 360
           E ED +P ENIICSLGKLFLNTGSSAAEIFVALLLGARRK S    ++HY+VNKHA SRF
Sbjct: 301 EAEDNQPEENIICSLGKLFLNTGSSAAEIFVALLLGARRKASDSHSREHYQVNKHAPSRF 360

Query: 361 GIQGSIAVPQGREPLHTMASKPYSYSAAQLENVHHYRRMWGDNGGRD 407
           G+Q + A   GREPL TM  KPYS S  ++ENV  Y+R+WG+NGGR+
Sbjct: 361 GVQENFAASYGREPLETMTRKPYSCSTTRVENVQRYKRIWGNNGGRE 406

BLAST of Lag0027739 vs. ExPASy TrEMBL
Match: A0A5A7UER2 (NHL domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G00630 PE=4 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 1.9e-186
Identity = 340/407 (83.54%), Postives = 365/407 (89.68%), Query Frame = 0

Query: 1   MARRWVLLAAIFGVLFCGFSSVSATPTKILAGVLSKAVSALVSWMWAISSASNTAVSTRS 60
           MARRWV+LA IF VLFCGFSSVSA PTKI+AG LSKAVSALV+W+W I+SASNT VSTRS
Sbjct: 1   MARRWVVLAVIFAVLFCGFSSVSAVPTKIVAGALSKAVSALVNWIWTITSASNTVVSTRS 60

Query: 61  MIKFEGGYVVETVFDGSKLGIEPYSVGVSPSGELLILDAENSNVHKISMPFSQFCRPKLL 120
           MIKFE GYVVET+ DGSK+GIEPYSVGVSPSGELLILDAENSNVHKISMP SQFCRPKL 
Sbjct: 61  MIKFERGYVVETLLDGSKMGIEPYSVGVSPSGELLILDAENSNVHKISMPVSQFCRPKLF 120

Query: 121 AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180
           AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW
Sbjct: 121 AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180

Query: 181 RKSGHVDGSSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRADDCTNEYDGSFLL 240
           RKSGH+DG SE+SKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRA+DCT EYDGSFLL
Sbjct: 181 RKSGHLDGPSEDSKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRAEDCT-EYDGSFLL 240

Query: 241 GMALLIAAMLSGYMLARFQFRVLATFSSKSDSRTDARNVTSIPPFRRPEKSVRHPLIPSE 300
           GMALL AAML GYMLARFQFRVLATFSSK+DSR  +RN+ SIPP+ R EKSVR PLIPSE
Sbjct: 241 GMALLTAAMLLGYMLARFQFRVLATFSSKNDSRVGSRNIPSIPPYGRVEKSVRRPLIPSE 300

Query: 301 EEED-EPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKPSHPQVQQHYRVNKHAQSRF 360
           EEED +P ENIICSLGKLFLNTGSSAAEIFVALLLGARRK S    ++HY+VNKHA SRF
Sbjct: 301 EEEDNQPEENIICSLGKLFLNTGSSAAEIFVALLLGARRKASDSHSREHYQVNKHAPSRF 360

Query: 361 GIQGSIAVPQGREPLHTMASKPYSYSAAQLENVHHYRRMWGDNGGRD 407
           G+Q + A   GRE L TM  KPYS S  +LE+V  Y+R+WGDNGGR+
Sbjct: 361 GVQENFAASYGRETLETMTRKPYSCSTTRLESVQRYKRIWGDNGGRE 406

BLAST of Lag0027739 vs. ExPASy TrEMBL
Match: A0A1S3B7P5 (uncharacterized protein LOC103487068 OS=Cucumis melo OX=3656 GN=LOC103487068 PE=4 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 1.9e-186
Identity = 340/407 (83.54%), Postives = 365/407 (89.68%), Query Frame = 0

Query: 1   MARRWVLLAAIFGVLFCGFSSVSATPTKILAGVLSKAVSALVSWMWAISSASNTAVSTRS 60
           MARRWV+LA IF VLFCGFSSVSA PTKI+AG LSKAVSALV+W+W I+SASNT VSTRS
Sbjct: 1   MARRWVVLAVIFAVLFCGFSSVSAVPTKIVAGALSKAVSALVNWIWTITSASNTVVSTRS 60

Query: 61  MIKFEGGYVVETVFDGSKLGIEPYSVGVSPSGELLILDAENSNVHKISMPFSQFCRPKLL 120
           MIKFE GYVVET+ DGSK+GIEPYSVGVSPSGELLILDAENSNVHKISMP SQFCRPKL 
Sbjct: 61  MIKFERGYVVETLLDGSKMGIEPYSVGVSPSGELLILDAENSNVHKISMPVSQFCRPKLF 120

Query: 121 AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180
           AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW
Sbjct: 121 AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180

Query: 181 RKSGHVDGSSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRADDCTNEYDGSFLL 240
           RKSGH+DG SE+SKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRA+DCT EYDGSFLL
Sbjct: 181 RKSGHLDGPSEDSKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRAEDCT-EYDGSFLL 240

Query: 241 GMALLIAAMLSGYMLARFQFRVLATFSSKSDSRTDARNVTSIPPFRRPEKSVRHPLIPSE 300
           GMALL AAML GYMLARFQFRVLATFSSK+DSR  +RN+ SIPP+ R EKSVR PLIPSE
Sbjct: 241 GMALLTAAMLLGYMLARFQFRVLATFSSKNDSRVGSRNIPSIPPYGRVEKSVRRPLIPSE 300

Query: 301 EEED-EPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKPSHPQVQQHYRVNKHAQSRF 360
           EEED +P ENIICSLGKLFLNTGSSAAEIFVALLLGARRK S    ++HY+VNKHA SRF
Sbjct: 301 EEEDNQPEENIICSLGKLFLNTGSSAAEIFVALLLGARRKASDSHSREHYQVNKHAPSRF 360

Query: 361 GIQGSIAVPQGREPLHTMASKPYSYSAAQLENVHHYRRMWGDNGGRD 407
           G+Q + A   GRE L TM  KPYS S  +LE+V  Y+R+WGDNGGR+
Sbjct: 361 GVQENFAASYGRETLETMTRKPYSCSTTRLESVQRYKRIWGDNGGRE 406

BLAST of Lag0027739 vs. ExPASy TrEMBL
Match: A0A6J1F447 (uncharacterized protein LOC111441944 OS=Cucurbita moschata OX=3662 GN=LOC111441944 PE=4 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 1.4e-179
Identity = 330/399 (82.71%), Postives = 360/399 (90.23%), Query Frame = 0

Query: 1   MARRWVLLAAIFGVLFCGFSSVSATPTKILAGVLSKAVSALVSWMWAISSASNTAVSTRS 60
           MA+RWVLLAAIF VL CGFSSVSAT T  +AGVLSKAVSALV+W+ AI+SAS+T VSTRS
Sbjct: 1   MAKRWVLLAAIFAVLVCGFSSVSATHTNFVAGVLSKAVSALVNWISAITSASSTVVSTRS 60

Query: 61  MIKFEGGYVVETVFDGSKLGIEPYSVGVSPSGELLILDAENSNVHKISMPFSQFCRPKLL 120
           MI FE GYVVET+FDGSKLGIEPYSVGVSPSGELLILD+ENSN+HKISMP SQFCRP+L 
Sbjct: 61  MITFEKGYVVETMFDGSKLGIEPYSVGVSPSGELLILDSENSNIHKISMPSSQFCRPQLF 120

Query: 121 AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180
           AGSSEGYSGHVDGK RDARMSHPRGL VDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW
Sbjct: 121 AGSSEGYSGHVDGKPRDARMSHPRGLAVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180

Query: 181 RKSGHVDGSSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRADDCTNEYDGSFLL 240
           RKSGH+DG SEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREI+LRA+DCT+E+DGSFLL
Sbjct: 181 RKSGHIDGPSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIRLRAEDCTDEFDGSFLL 240

Query: 241 GMALLIAAMLSGYMLARFQFRVLATFSSKSDSRTDARNVTSIPPFRRPEKSVRHPLIPSE 300
           GMALL AAML GYMLAR QF VLA FSSK+DSR D+R+V+SIPP+RR EKS+RHPLIPSE
Sbjct: 241 GMALLTAAMLLGYMLARLQFWVLAAFSSKNDSRADSRDVSSIPPYRRVEKSMRHPLIPSE 300

Query: 301 EEED-EPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKPSHPQVQQHYRVNKHAQSRF 360
           EEED +PGENIICSLGKLFLNTGSSAAEIFVALLLGARRK ++  VQQHY+VNKH Q+  
Sbjct: 301 EEEDNQPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKAANLHVQQHYQVNKHGQAGL 360

Query: 361 GIQGSIAVPQGREPLHTMASKPYSYSAAQLENVHHYRRM 399
           G+Q S A+P GREPLHTM    YSYSA + ENV  YRR+
Sbjct: 361 GMQESFAIPCGREPLHTMTGIHYSYSATRPENVQRYRRI 399

BLAST of Lag0027739 vs. ExPASy TrEMBL
Match: A0A6J1J758 (uncharacterized protein LOC111481869 OS=Cucurbita maxima OX=3661 GN=LOC111481869 PE=4 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 8.2e-177
Identity = 328/404 (81.19%), Postives = 355/404 (87.87%), Query Frame = 0

Query: 1   MARRWVLLAAIFGVLFCGFSSVSATPTKILAGVLSKAVSALVSWMWAISSASNTAVSTRS 60
           MA+RWVLLAAIF VL CGFSSVSAT T  +AGVLSKAVSALV+W+ A +SAS+T VSTRS
Sbjct: 1   MAKRWVLLAAIFAVLVCGFSSVSATHTNFVAGVLSKAVSALVNWISAFTSASSTVVSTRS 60

Query: 61  MIKFEGGYVVETVFDGSKLGIEPYSVGVSPSGELLILDAENSNVHKISMPFSQFCRPKLL 120
           MI FE GYVVETVFDGSKLGIEPYSVGVSPSGELLILD+ENSN+HKISMP SQFCRP+L 
Sbjct: 61  MITFEKGYVVETVFDGSKLGIEPYSVGVSPSGELLILDSENSNIHKISMPSSQFCRPQLF 120

Query: 121 AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180
           AGSSEGYSGHVDGK RDARMSHPRGL VDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW
Sbjct: 121 AGSSEGYSGHVDGKPRDARMSHPRGLAVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180

Query: 181 RKSGHVDGSSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRADDCTNEYDGSFLL 240
           RKSGH+DG SEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREI+LRADDCT+E+DGSFLL
Sbjct: 181 RKSGHIDGPSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIRLRADDCTDEFDGSFLL 240

Query: 241 GMALLIAAMLSGYMLARFQFRVLATFSSKSDSRTDARNVTSIPPFRRPEKSVRHPLIPSE 300
           GMALL AAML GYMLAR QF VLA FSSK+DSR D+R+V+SIPP+   EKSVRHPLIPSE
Sbjct: 241 GMALLTAAMLLGYMLARLQFWVLAAFSSKNDSRADSRDVSSIPPYLTAEKSVRHPLIPSE 300

Query: 301 EEED-EPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKPSHPQVQQHYRVNKHAQSRF 360
           EEE+ +PGENIICSLGKLFLNTGSSAAEIFVALLLG RRK + P VQQHY+VNK   +  
Sbjct: 301 EEEENQPGENIICSLGKLFLNTGSSAAEIFVALLLGDRRKAADPHVQQHYQVNKRGPAGL 360

Query: 361 GIQGSIAVPQGREPLHTMASKPYSYSAAQLENVHHYRRMWGDNG 404
           G+Q S A+P GREPLHTM    YSYSA   E V  YRR+ G +G
Sbjct: 361 GMQESFAIPCGREPLHTMTGIHYSYSATCPEKVQRYRRIRGRDG 404

BLAST of Lag0027739 vs. TAIR 10
Match: AT1G70280.2 (NHL domain-containing protein )

HSP 1 Score: 348.6 bits (893), Expect = 7.0e-96
Identity = 200/411 (48.66%), Postives = 268/411 (65.21%), Query Frame = 0

Query: 1   MARRWVLLAAIFGVLFCGFSSVSA--TPTKILAGVLSKAVSALVSWMWAISSASNTAVST 60
           M R +++L+ +  +L    SS  +  +P KIL G +S   S+L+ W+W++ + + T ++T
Sbjct: 1   MGRDFLVLSILILLLSGIVSSAPSANSPAKILNGFISNHGSSLMKWLWSLKTTTKTTIAT 60

Query: 61  RSMIKFEGGYVVETVFDGSKLGIEPYSVGVSPSGELLILDAENSNVHKISMPFSQFCRPK 120
           RSM+KFE GY VETVFDGSKLGIEPYS+ V P+GELLILD+ENSN++KIS   S + RP+
Sbjct: 61  RSMVKFENGYSVETVFDGSKLGIEPYSIEVLPNGELLILDSENSNIYKISSSLSLYSRPR 120

Query: 121 LLAGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGG 180
           L+ GS EGY GHVDG+LRDA+++HP+GLTVD RGNIY+ADT N AIRKIS+ GVTTIAGG
Sbjct: 121 LVTGSPEGYPGHVDGRLRDAKLNHPKGLTVDDRGNIYVADTVNNAIRKISEGGVTTIAGG 180

Query: 181 KW-RKSGHVDGSSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRADDCTNEYDGS 240
           K  R  GHVDG SE++KFSNDFDVVYVGSSCSLLV+DRGN+AIREIQL  DDC  +Y   
Sbjct: 181 KTVRNGGHVDGPSEDAKFSNDFDVVYVGSSCSLLVIDRGNKAIREIQLHFDDCAYQYGSG 240

Query: 241 FLLGMALLIAAMLSGYMLARFQFRVLATFSSKSDSRTDARNVTSIPPFRRPEKSVRHPLI 300
           F LG+A+L+AA   GYMLA  Q RV +  SS +D     + +    P ++P K  R  LI
Sbjct: 241 FPLGIAVLVAAGFFGYMLALLQRRVGSIVSSHND-----QEMFEADPDQKPMKHSRPSLI 300

Query: 301 PSEEEE-DEPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKPSHPQVQQHYRVNKHA- 360
           P+ +E+ ++  E  + SLGKL  N   S  EI        R+K +    QQ++   K + 
Sbjct: 301 PAGDEQLEKQEETFVVSLGKLVSNAWESVMEIL-------RKKQTGTSFQQYHGTTKQSA 360

Query: 361 ----QSRFGIQGSIAV-----PQGREPLHTMASKPYSYSAAQLENVHHYRR 398
                + + IQ S  +     P   EP +    K Y++ +   E +   R+
Sbjct: 361 AFSTSTPWPIQESFVIRDEDGPPPVEPRNPTPRKTYAFMSKDAEKMQQLRQ 399

BLAST of Lag0027739 vs. TAIR 10
Match: AT5G14890.1 (NHL domain-containing protein )

HSP 1 Score: 329.7 bits (844), Expect = 3.4e-90
Identity = 201/414 (48.55%), Postives = 273/414 (65.94%), Query Frame = 0

Query: 6   VLLAAIFGVLFCGFSSVSA--TPTKILAGVLSKAVSALVSWMWAISSASNT------AVS 65
           ++L  IF   FCG SSVSA   P KI++G+++   S L  W+W++ +++ T       VS
Sbjct: 8   LILFMIFMFFFCGLSSVSAKPPPVKIVSGLVTNVASILWKWLWSLQTSTTTTTTTKSGVS 67

Query: 66  TRSMIKFEGGYVVETVFDGSKLGIEPYSVGVSPS-GELLILDAENSNVHKISMPFSQFCR 125
           +RSM+K+E GY +ETVFDGSKLGIEPY++ VSP+ GEL++LD+ENSN+HKISMP S++ +
Sbjct: 68  SRSMVKYESGYNMETVFDGSKLGIEPYAIEVSPNGGELIVLDSENSNIHKISMPLSRYGK 127

Query: 126 PKLLAGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTI- 185
           PKLL+GS EGY+GHVDGKL++ARM+ PRGL +D RGNIY+ADT N AIRKISD GV+TI 
Sbjct: 128 PKLLSGSQEGYTGHVDGKLKEARMNRPRGLAMDDRGNIYVADTINMAIRKISDDGVSTIA 187

Query: 186 AGGKWRKSGHVDGSSEES-KFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRADDCTN-E 245
           AGG+W  SG   GS EES +FS+DFD++YV SSCSLLV+DRGNQ I+EIQL   DC+  E
Sbjct: 188 AGGRW--SG---GSKEESMRFSDDFDLIYVSSSCSLLVIDRGNQLIKEIQLHDHDCSQPE 247

Query: 246 YD-GSFLLGMALLIAAMLSGYMLARFQFRVLATFSSKSDSRTDARNVT----SIPPFRRP 305
            D  S  LG ALL+AA+  GYMLA    RV + FSS S      R+V     ++ P++R 
Sbjct: 248 PDTDSLHLGTALLVAAVFFGYMLALLVRRVRSLFSSSSHDTKSKRHVATPSMTMAPYQRY 307

Query: 306 EKSVRHPLIPSEEEEDEPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKPSHPQVQQH 365
            + VR PLIP  + E E  E  + SLGKL + TGSS +E     ++   R    P   Q+
Sbjct: 308 PRPVRQPLIP-PQHESEKEEGFLGSLGKLVVKTGSSVSE-----MMSGSRNVIPPNFHQY 367

Query: 366 YRVNKHAQSRFGIQGSIAVPQGREP------LHTMASKPYSYSAAQLENVHHYR 397
           +  ++   +++ +Q S A+P+   P        T   KPY  +    +N  +Y+
Sbjct: 368 H--HQQEPNQWPVQESFAIPEEDGPPALEPRSGTNPDKPYLRAQGTNQNRSYYQ 408

BLAST of Lag0027739 vs. TAIR 10
Match: AT1G23880.1 (NHL domain-containing protein )

HSP 1 Score: 329.3 bits (843), Expect = 4.4e-90
Identity = 188/382 (49.21%), Postives = 259/382 (67.80%), Query Frame = 0

Query: 1   MARRWVLLAAIFGVLFCGF---SSVSATPTKILAGVLSKAVSALVSWMWAIS--SASNTA 60
           M+R ++ L  I  +LF  F   +  S +P KI+   +S   ++L+ W+W++S  + + TA
Sbjct: 61  MSRHFLFLGIII-LLFSAFVASAPSSTSPAKIVNSFISNHGTSLLKWLWSLSFKTTTKTA 120

Query: 61  VSTRSMIKFEGGYVVETVFDGSKLGIEPYSVGVSPSGELLILDAENSNVHKISMPFSQFC 120
           V T+SM+KFE GY VETV DGSKLGIEPYS+ V  +GELLILD++NSN+++IS   S + 
Sbjct: 121 VPTKSMVKFENGYSVETVLDGSKLGIEPYSIQVLSNGELLILDSQNSNIYQISSSLSLYS 180

Query: 121 RPKLLAGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTI 180
           RP+L+ GS EGY GHVDG+LRDAR+++P+GLTVD RGNIY+ADT N AIRKIS+AGVTTI
Sbjct: 181 RPRLVTGSPEGYPGHVDGRLRDARLNNPKGLTVDDRGNIYVADTVNNAIRKISEAGVTTI 240

Query: 181 AGGKW-RKSGHVDGSSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRADDCTNEY 240
           AGGK  R  GHVDG SE++KFSNDFDVVY+GSSCSLLV+DRGNQAIREIQL  DDC ++Y
Sbjct: 241 AGGKMVRGGGHVDGPSEDAKFSNDFDVVYLGSSCSLLVIDRGNQAIREIQLHFDDCADQY 300

Query: 241 DGSFLLGMALLIAAMLSGYMLARFQFRVLATFSSKSDSRTDARNVTSIPPFRRPEKSVRH 300
              F LG+A+L+AA+  GYMLA  Q R+ +  S  +D     + V    P + P K VR 
Sbjct: 301 GSGFPLGIAVLVAAVFFGYMLALLQRRLSSIVSYHTD-----QEVFEAVPDQDPIKPVRP 360

Query: 301 PLIPSEEEEDEPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKPSHPQVQQHYRVNKH 360
           PLI + +E+++  E+ + +L     N    + E+F  +  G R+K +   +  +++  KH
Sbjct: 361 PLILTGDEQEKQEESFLGTLQIFISNAWVFSVELFSGMFPGLRKKQT-VGLNFNHQETKH 420

Query: 361 ---AQSRFGIQGSIAVPQGREP 374
              + + + IQ S  +    EP
Sbjct: 421 SAFSTTSWPIQESFVIHNKDEP 435

BLAST of Lag0027739 vs. TAIR 10
Match: AT1G70280.1 (NHL domain-containing protein )

HSP 1 Score: 319.7 bits (818), Expect = 3.5e-87
Identity = 180/349 (51.58%), Postives = 230/349 (65.90%), Query Frame = 0

Query: 61  MIKFEGGYVVETVFDGSKLGIEPYSVGVSPSGELLILDAENSNVHKISMPFSQFCRPKLL 120
           M+KFE GY VETVFDGSKLGIEPYS+ V P+GELLILD+ENSN++KIS   S + RP+L+
Sbjct: 1   MVKFENGYSVETVFDGSKLGIEPYSIEVLPNGELLILDSENSNIYKISSSLSLYSRPRLV 60

Query: 121 AGSSEGYSGHVDGKLRDARMSHPRGLTVDQRGNIYIADTKNKAIRKISDAGVTTIAGGKW 180
            GS EGY GHVDG+LRDA+++HP+GLTVD RGNIY+ADT N AIRKIS+ GVTTIAGGK 
Sbjct: 61  TGSPEGYPGHVDGRLRDAKLNHPKGLTVDDRGNIYVADTVNNAIRKISEGGVTTIAGGKT 120

Query: 181 -RKSGHVDGSSEESKFSNDFDVVYVGSSCSLLVVDRGNQAIREIQLRADDCTNEYDGSFL 240
            R  GHVDG SE++KFSNDFDVVYVGSSCSLLV+DRGN+AIREIQL  DDC  +Y   F 
Sbjct: 121 VRNGGHVDGPSEDAKFSNDFDVVYVGSSCSLLVIDRGNKAIREIQLHFDDCAYQYGSGFP 180

Query: 241 LGMALLIAAMLSGYMLARFQFRVLATFSSKSDSRTDARNVTSIPPFRRPEKSVRHPLIPS 300
           LG+A+L+AA   GYMLA  Q RV +  SS +D     + +    P ++P K  R  LIP+
Sbjct: 181 LGIAVLVAAGFFGYMLALLQRRVGSIVSSHND-----QEMFEADPDQKPMKHSRPSLIPA 240

Query: 301 EEEE-DEPGENIICSLGKLFLNTGSSAAEIFVALLLGARRKPSHPQVQQHYRVNKHA--- 360
            +E+ ++  E  + SLGKL  N   S  EI        R+K +    QQ++   K +   
Sbjct: 241 GDEQLEKQEETFVVSLGKLVSNAWESVMEIL-------RKKQTGTSFQQYHGTTKQSAAF 300

Query: 361 --QSRFGIQGSIAV-----PQGREPLHTMASKPYSYSAAQLENVHHYRR 398
              + + IQ S  +     P   EP +    K Y++ +   E +   R+
Sbjct: 301 STSTPWPIQESFVIRDEDGPPPVEPRNPTPRKTYAFMSKDAEKMQQLRQ 337

BLAST of Lag0027739 vs. TAIR 10
Match: AT3G14860.2 (NHL domain-containing protein )

HSP 1 Score: 232.3 bits (591), Expect = 7.3e-61
Identity = 133/308 (43.18%), Postives = 192/308 (62.34%), Query Frame = 0

Query: 31  AGVLSKAVSALVSWMWAISSASNTAVSTRSMIKFEGGYVVETVFDGSKLGIEPYSVGVSP 90
           +G L K +S+++   W   S+S  + S  ++++FE GY+VETV +G+ +G+ PY + VS 
Sbjct: 42  SGSLIKHMSSVLK--WTTGSSSKLSQSDTNVLQFENGYLVETVVEGNDIGVVPYKIRVSD 101

Query: 91  SGELLILDAENSNVHKISMPFSQFCRPKLLAGSSEGYSGHVDGKLRDARMSHPRGLTVDQ 150
            GEL  +D  NSN+ KI+ P SQ+ R +L+AGS +G +GH DGK  +AR +HPRG+T+D 
Sbjct: 102 DGELYAVDELNSNIMKITPPLSQYSRGRLVAGSFQGKTGHADGKPSEARFNHPRGVTMDD 161

Query: 151 RGNIYIADTKNKAIRKISDAGVTTIAGGKWRKSGHVDGSSEESKFSNDFDVVYVGSSCSL 210
           +GN+Y+ADT N AIRKI D+GVTTIAGGK   +G+ DG SE++KFSNDFDVVYV  +CSL
Sbjct: 162 KGNVYVADTLNLAIRKIGDSGVTTIAGGKSNIAGYRDGPSEDAKFSNDFDVVYVRPTCSL 221

Query: 211 LVVDRGNQAIREIQLRADDCTNEYDGSF-LLGMALLIAAMLSGYMLARFQFRVLATFSSK 270
           LV+DRGN A+R+I L  +DC  + D S  L  + L+I A+L GY     Q      F + 
Sbjct: 222 LVIDRGNAALRQISLSEEDCDYQDDSSISLTDILLVIGAVLIGYATCMLQ----QGFGNS 281

Query: 271 SDSRTDARNVTSIPPFRRPEKSVRHPLIPSEEEEDEPGENIICSLGKLFLNTGSSAAEIF 330
             S+T   + TS       ++ +  P+      ++EPG     S G+L  +    A E  
Sbjct: 282 FFSKTQVGSETSYEEEHPGKEKLSRPVHEKTITKEEPGWP---SFGQLLTDLCKLALEFI 340

Query: 331 VALLLGAR 338
            + L+ AR
Sbjct: 342 TSHLVPAR 340

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903583.19.6e-18883.70uncharacterized protein LOC120090138 [Benincasa hispida][more]
XP_004141183.23.6e-18783.78uncharacterized protein LOC101216407 [Cucumis sativus] >KGN59643.1 hypothetical ... [more]
XP_008443491.14.0e-18683.54PREDICTED: uncharacterized protein LOC103487068 [Cucumis melo] >KAA0053690.1 NHL... [more]
KAG6581010.12.1e-18283.17hypothetical protein SDJN03_21012, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022934912.12.8e-17982.71uncharacterized protein LOC111441944 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q8VZ105.6e-0528.10Protein SUPPRESSOR OF QUENCHING 1, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
A4IF699.5e-0530.00NHL repeat-containing protein 2 OS=Bos taurus OX=9913 GN=NHLRC2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LCX91.8e-18783.78Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G835800 PE=4 SV=1[more]
A0A5A7UER21.9e-18683.54NHL domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A1S3B7P51.9e-18683.54uncharacterized protein LOC103487068 OS=Cucumis melo OX=3656 GN=LOC103487068 PE=... [more]
A0A6J1F4471.4e-17982.71uncharacterized protein LOC111441944 OS=Cucurbita moschata OX=3662 GN=LOC1114419... [more]
A0A6J1J7588.2e-17781.19uncharacterized protein LOC111481869 OS=Cucurbita maxima OX=3661 GN=LOC111481869... [more]
Match NameE-valueIdentityDescription
AT1G70280.27.0e-9648.66NHL domain-containing protein [more]
AT5G14890.13.4e-9048.55NHL domain-containing protein [more]
AT1G23880.14.4e-9049.21NHL domain-containing protein [more]
AT1G70280.13.5e-8751.58NHL domain-containing protein [more]
AT3G14860.27.3e-6143.18NHL domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001258NHL repeatPFAMPF01436NHLcoord: 141..166
e-value: 4.7E-5
score: 23.2
IPR011042Six-bladed beta-propeller, TolB-likeGENE3D2.120.10.30coord: 24..224
e-value: 1.4E-31
score: 112.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 273..305
NoneNo IPR availablePANTHERPTHR13833:SF78POTASSIUM TRANSPORTERcoord: 1..406
NoneNo IPR availablePANTHERPTHR13833FAMILY NOT NAMEDcoord: 1..406
NoneNo IPR availableSUPERFAMILY101898NHL repeatcoord: 59..226

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0027739.1Lag0027739.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005515 protein binding