Lag0027730 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0027730
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase
Locationchr8: 4177810 .. 4179293 (+)
RNA-Seq ExpressionLag0027730
SyntenyLag0027730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTGCTAAACAAGCATGGCGTGTGGTGGTTAATCCAAACTTAACGCTCTCTAAGATTCTAAGGGGGAGGTATTTCCCTACGTTGTCTGTTTTTTATGCATCAGTCAAATCTTCCTCCTCTTATTTCTGGAAGGGTTTTGTGTTGGGGCTTGGATTTGCTGAAGGGGGTATTCGACGGAATCGAGCGAATGGTCAGTCGATTAAAATCTTTAAAGATCCTTGGATTCCTCATCCTGTTACCTTTAAGGTGATGATGATCGAAGGCTAGGCTAATGAGGATATGAGAGTGACAGAATTTATCACTTCATCAAACCAGTGGGGTGTCTCCAAGCTTAACCAATTTCTATGCGATGAGGATGTTCGAGTGATTTCTCACTTACCAATTAACAGGTCAGCTCTGGATACGTGTATTTGGCACTATGTCAGGAAAGGGAAATATATTGTCAAGATTGGGTATAAGTATAGCATGTTACAGCAATAGGCAACTTCTTCTTCGAATCTTGAAACGAAAAGTAACTGGTGGAAGAAATTATGGAAGTTGAGGATTCCAAACAAGGTCAAACACTTCGTCTGGAACTCATTCCATAATTAGATTTGACAATGGCTAACCTGTGGCGTCACCATGTCCCAGTGGATGGTACTTGTACCAGTTGAAAAGAGGGCCTGGAAACTACTGATCATGCTCTATTTCAGTATAAGCGTGTGATGAAGATATGGGAAGTTCTCGTTCTAGGTGAAATCAAGACCATTCAAAGACATATGGATATATAGGATCGTTGGCTTTATCTCAGTAGTTGCTCTGAGCCCGTTTTGGATCTAATTTATATTGGTGCCTGGGCAATTTGGAATGATAGAAATTATGCCCTCCATAACCGCCTTGTTCCCAGCATCATTGATAGGTGTGTATGGATTTATAGGTACCTTGAAGACTTTAGAAAGATTAATGAGGTTAGGGCTTCTGTGTCATCATCTAGGGTCGACTTACATGTATTAGTCGACGAAGGCGAGGAAACCTTTATGCATACTGATGCGGCTTTTAATGAACCCAATAAGAAGTCAGAAATTGGGGTAGTGCTTCGTGACAAACAAGGTGATTTAAAGGCAGTTCTGTCTTCACCATCCCAAGTTTGTATCTCCCATCTAGGTGCTGAAGTTGTTGCTGTACTGGAGGGAATTCGATCCGCTATTAGTATGGGTGTCGAAAGCATTTTAGTGATGTGCGACTCTCTTTCATTGGTCAAGGCTCTTCAGGGAACGAACCATTGTGATTCGGGTATTTCATCAGTTTTGTGGGATACAAAAGGTCTTCAGACTTCTTTTCGTAGGATAACTTTTTTATCATGTAAACCGTTAATTTAATAAGTTTGCCCACGAATTGCCCTAGGTGGGCCAATATTCTACTAGGAAGGAGTGGAAGTCACATTATCCTGAGTGGATCACTCGGATGATGTGTCAGGAGCGTTCTCTTTTTGTATCCCCTAG

mRNA sequence

ATGTTTGCTAAACAAGCATGGCGTGTGGTGGTTAATCCAAACTTAACGCTCTCTAAGATTCTAAGGGGGAGGTATTTCCCTACGTTGTCTGTTTTTTATGCATCAGTCAAATCTTCCTCCTCTTATTTCTGGAAGGGTTTTGTGTTGGGGCTTGGATTTGCTGAAGGGGGTATTCGACGGAATCGAGCGAATGGTCAGTCGATTAAAATCTTTAAAGATCCTTGGATTCCTCATCCTGTTACCTTTAAGTGGGGTGTCTCCAAGCTTAACCAATTTCTATGCGATGAGGATGTTCGAGTGATTTCTCACTTACCAATTAACAGTAGTTGCTCTGAGCCCGTTTTGGATCTAATTTATATTGGTGCCTGGGCAATTTGGAATGATAGAAATTATGCCCTCCATAACCGCCTTGTTCCCAGCATCATTGATAGGTGTGTATGGATTTATAGGTACCTTGAAGACTTTAGAAAGATTAATGAGGTTAGGGCTTCTGTGTCATCATCTAGGGTCGACTTACATGTATTAGTCGACGAAGGCGAGGAAACCTTTATGCATACTGATGCGGCTTTTAATGAACCCAATAAGAAGTCAGAAATTGGGGTAGTGCTTCGTGACAAACAAGGTGATTTAAAGGCAGTTCTGTCTTCACCATCCCAAGTTTGTATCTCCCATCTAGGTGCTGAAGTTGTTGCTGTACTGGAGGGAATTCGATCCGCTATTAGTATGGGTGTCGAAAGCATTTTAGTGATGTGCGACTCTCTTTCATTGGTCAAGGCTCTTCAGGGAACGAACCATTGTGATTCGGGTATTTCATCAGTTTTGTGGGATACAAAAGGAGCGTTCTCTTTTTGTATCCCCTAG

Coding sequence (CDS)

ATGTTTGCTAAACAAGCATGGCGTGTGGTGGTTAATCCAAACTTAACGCTCTCTAAGATTCTAAGGGGGAGGTATTTCCCTACGTTGTCTGTTTTTTATGCATCAGTCAAATCTTCCTCCTCTTATTTCTGGAAGGGTTTTGTGTTGGGGCTTGGATTTGCTGAAGGGGGTATTCGACGGAATCGAGCGAATGGTCAGTCGATTAAAATCTTTAAAGATCCTTGGATTCCTCATCCTGTTACCTTTAAGTGGGGTGTCTCCAAGCTTAACCAATTTCTATGCGATGAGGATGTTCGAGTGATTTCTCACTTACCAATTAACAGTAGTTGCTCTGAGCCCGTTTTGGATCTAATTTATATTGGTGCCTGGGCAATTTGGAATGATAGAAATTATGCCCTCCATAACCGCCTTGTTCCCAGCATCATTGATAGGTGTGTATGGATTTATAGGTACCTTGAAGACTTTAGAAAGATTAATGAGGTTAGGGCTTCTGTGTCATCATCTAGGGTCGACTTACATGTATTAGTCGACGAAGGCGAGGAAACCTTTATGCATACTGATGCGGCTTTTAATGAACCCAATAAGAAGTCAGAAATTGGGGTAGTGCTTCGTGACAAACAAGGTGATTTAAAGGCAGTTCTGTCTTCACCATCCCAAGTTTGTATCTCCCATCTAGGTGCTGAAGTTGTTGCTGTACTGGAGGGAATTCGATCCGCTATTAGTATGGGTGTCGAAAGCATTTTAGTGATGTGCGACTCTCTTTCATTGGTCAAGGCTCTTCAGGGAACGAACCATTGTGATTCGGGTATTTCATCAGTTTTGTGGGATACAAAAGGAGCGTTCTCTTTTTGTATCCCCTAG

Protein sequence

MFAKQAWRVVVNPNLTLSKILRGRYFPTLSVFYASVKSSSSYFWKGFVLGLGFAEGGIRRNRANGQSIKIFKDPWIPHPVTFKWGVSKLNQFLCDEDVRVISHLPINSSCSEPVLDLIYIGAWAIWNDRNYALHNRLVPSIIDRCVWIYRYLEDFRKINEVRASVSSSRVDLHVLVDEGEETFMHTDAAFNEPNKKSEIGVVLRDKQGDLKAVLSSPSQVCISHLGAEVVAVLEGIRSAISMGVESILVMCDSLSLVKALQGTNHCDSGISSVLWDTKGAFSFCIP
Homology
BLAST of Lag0027730 vs. NCBI nr
Match: XP_030939737.1 (uncharacterized protein LOC115964598 [Quercus lobata])

HSP 1 Score: 107.5 bits (267), Expect = 2.0e-19
Identity = 81/310 (26.13%), Postives = 131/310 (42.26%), Query Frame = 0

Query: 1   MFAKQAWRVVVNPNLTLSKILRGRYFPTLSVFYASVKSSSSYFWKGFVLGLGFAEGGIRR 60
           + AKQ WR+       + ++ + +YFP     +AS+ +  SY W+  +      + G+R 
Sbjct: 237 LLAKQGWRLQTQHESLVYRVYKAKYFPNTGFIHASLGTHPSYTWRSLMAAQHLVKKGVRW 296

Query: 61  NRANGQSIKIFKDPWIPHPVTFKWGVSKLNQFLCDEDVR--------------------- 120
           N  NG SI ++ D W+P P TFK    +L      EDVR                     
Sbjct: 297 NVGNGDSIWVWSDKWLPSPSTFKITSPRLAM----EDVRSSNRGSSSDPSKTRRFWKRLW 356

Query: 121 ---VISHLPINSSCSEPVLDLIYIGAWAIWNDRNYALHNRLVPSIIDRCVWIYRYLEDFR 180
              V   + +  S  E    L+   AW +W +RN   H     S +D   W  +YL++F+
Sbjct: 357 RLQVPHKMLMMDSHEEKDAKLVVTIAWMLWFNRNEVRHGGRKKSAVDLFQWSRQYLQEFQ 416

Query: 181 KINEVRASVSSSRVDLHVLVDEGEETFMHTDAAFNEPNKKSEIGVVLRDKQGDLKAVLSS 240
           ++N+V  S     V +           ++ D       K + +GV++RD  G + A LS 
Sbjct: 417 EVNQVVPSTGMPWV-VGWSPPPLTRYKVNVDDTVFTAQKCAGVGVLIRDSYGQVIAALSR 476

Query: 241 PSQVCISHLGAEVVAVLEGIRSAISMGVESILVMCDSLSLVKALQGTNHCDSGISSVLWD 287
                +  L  E  A   G+  A  +GV   ++  DSL +  AL G++   S I+ V+  
Sbjct: 477 KLNAPLGALEVEAKAFEVGLEFARDVGVHDFVLEGDSLVVYNALCGSSIPPSMIAPVI-- 536

BLAST of Lag0027730 vs. NCBI nr
Match: XP_031099874.1 (uncharacterized protein LOC116004082 [Ipomoea triloba])

HSP 1 Score: 100.9 bits (250), Expect = 1.9e-17
Identity = 81/294 (27.55%), Postives = 128/294 (43.54%), Query Frame = 0

Query: 1    MFAKQAWRVVVNPNLTLSKILRGRYFPTLSVFYASVKSSSSYFWKGFVLGLGFAEGGIRR 60
            M  KQ WR + NP+  +++I + RYFP  S   ASV    SY W+  +        G+RR
Sbjct: 834  MLGKQVWRFLTNPHSLVARIYKARYFPKSSFIDASVGGCPSYCWRSIMAAHDLVCSGVRR 893

Query: 61   NRANGQSIKIFKDPWIP------------------------HPVTFKWGVSKLNQFLCDE 120
               NG+S  I+  PW+P                         P T  W  + L+      
Sbjct: 894  RIGNGKSTLIWGHPWLPDDPGPMVQTVMPQTLAGAAVSGLIDPTTNTWDQTILHDIFTLN 953

Query: 121  DVRVISHLPINSSCSEPVLDLIYIGAWAIWNDRNYALHNRLVPSIIDRCVW--IYRYLED 180
            DVR I  +P  ++ +     ++Y     IW  RN A+ +  +P+   R VW       + 
Sbjct: 954  DVRRILSIPFLAAVA-----VLYF----IWTARNKAVWDACLPT--PRRVWQMATSAADA 1013

Query: 181  FRKINEVRASVSS-----SRVDLHVLVDEGEETFMHT---DAAFNEPNKKSEIGVVLRDK 240
            +R++++     S+     +   L   V +G+   +H    DA F   ++K+ +GVVL   
Sbjct: 1014 WRQVHQHMPGPSTPDGVEAGATLSAAVHQGD--MLHRCFFDAGFLPKSRKASVGVVLLSP 1073

Query: 241  QGDLKAVLSSPSQVCISHLGAEVVAVLEGIRSAISMGVESILVMCDSLSLVKAL 261
            +GD  A  +SP Q C+S L AE VA  E +      G+ S++   D   L + L
Sbjct: 1074 EGDFVAAFNSPMQDCLSPLMAESVACKEVLSWIKDRGLGSVVHFTDCSGLQQLL 1114

BLAST of Lag0027730 vs. NCBI nr
Match: XP_024178198.1 (uncharacterized protein LOC112184155 [Rosa chinensis])

HSP 1 Score: 100.9 bits (250), Expect = 1.9e-17
Identity = 71/305 (23.28%), Postives = 127/305 (41.64%), Query Frame = 0

Query: 1   MFAKQAWRVVVNPNLTLSKILRGRYFPTLSVFYASVKSSSSYFWKGFVLGLGFAEGGIRR 60
           + AKQ WR++  P+  L+++L+ RYFP      A +   +S+ W+  + G      G+R 
Sbjct: 157 LLAKQGWRLICRPDSLLAQVLKARYFPQTDFMQAELHKGASFTWRSILKGRDLLRKGLRF 216

Query: 61  NRANGQSIKIFKDPWIPHPVTFK------WGVS--KLNQFLCDEDVRVISHLPIN----- 120
              NG++I ++ DPW+P P  F+       G    K+  F+  E      H  +N     
Sbjct: 217 QVGNGENISVWNDPWVPLPYRFRPFSLPMQGTEEFKVADFIDSEAGAFWLHGLLNLQPGK 276

Query: 121 --------------SSCSEPVLDLIYIGAWAIWNDRNYALHNRLVPSIIDRCVWIYRYLE 180
                          +     L+   +  W +W +RN  +      +II+   W   +L+
Sbjct: 277 HPTTLLDIWFWDMIEALDGEKLEYFLMALWVVWMERNNMVWKGSYFNIINMHAWATSFLD 336

Query: 181 DFRKINEVRASVSSSRVDLHVLVDEGEETFMHTDAAFNEPNKKSEIGVVLRDKQGDLKAV 240
           D++ +++ R    + R  +           ++ D +F        +GVV+R+ +G   A 
Sbjct: 337 DYKNLHK-RQGHKARRTPIKWSRPPRGRLKINIDGSFCADTGSGGVGVVVRNYEGTCMAT 396

Query: 241 LSSPSQVCISHLGAEVVAVLEGIRSAISMGVESILVMCDSLSLVKALQGTNHCDSGISSV 279
            +   Q   S +  E  A+  G+  AI    + I V  D ++LV AL       S I  +
Sbjct: 397 FARTVQYAQSAIHMEAEALRAGLLIAIHQDWKEIEVESDCVNLVNALHVDGDDLSDIGRI 456

BLAST of Lag0027730 vs. NCBI nr
Match: XP_042973059.1 (uncharacterized protein LOC122304861 [Carya illinoinensis])

HSP 1 Score: 100.1 bits (248), Expect = 3.2e-17
Identity = 79/330 (23.94%), Postives = 138/330 (41.82%), Query Frame = 0

Query: 1   MFAKQAWRVVVNPNLTLSKILRGRYFPTLSVFYASVKSSSSYFWKGFVLGLGFAEGGIRR 60
           + AKQ WRV++NP    + +L+ +YF    + +A++    S+ WK     L   + G+  
Sbjct: 22  LLAKQCWRVLMNPQSLAAVLLKDKYFRNTDLLHATLGFKPSFMWKSLWDSLSLLKEGLVW 81

Query: 61  NRANGQSIKIFKDPWIPHPVTFK------------------------WGVSKLNQFLCDE 120
              NG+SIKI+ D W+P  VTFK                        W    + Q    E
Sbjct: 82  RVGNGKSIKIWGDRWVPKAVTFKIQSPIQILNAEARVEELLSGENGGWNEGLIKQVFDKE 141

Query: 121 DVRVISHLPINSS----------------------CSEPVLDLIYIGAWAIWNDRN-YAL 180
           +  ++ +LPI+ S                        E  ++LI +    IW  RN +  
Sbjct: 142 EAAIVCNLPISFSKLPDKQIWAFSKNGIYNVKSAYHVEDQMELIAVVLRRIWFRRNGWVF 201

Query: 181 HNRL-----VPSIIDRCVWIYRYLEDFRKINEVRASVSSSRVDLHVLVDEGEETFMHTDA 240
            NR      V S    C+  Y+  ++ ++    +++    R  LH    EG+   ++ DA
Sbjct: 202 ENRFEGPNDVFSQAANCLSEYQQAQETQQAQPKQSNSQCGRA-LHWKPPEGDTVKVNWDA 261

Query: 241 AFNEPNKKSEIGVVLRDKQGDLKAVLSSPSQVCISHLGAEVVAVLEGIRSAISMGVESIL 279
           A    ++K  +GVV+RD+ GD+ A L S      + + AEV A+   ++    +    + 
Sbjct: 262 ALKSDDRKCGMGVVIRDRCGDVLASLCSCRWNVSNPVVAEVHALWRAMKLCEELKFSKVQ 321

BLAST of Lag0027730 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 100.1 bits (248), Expect = 3.2e-17
Identity = 49/130 (37.69%), Postives = 68/130 (52.31%), Query Frame = 0

Query: 1    MFAKQAWRVVVNPNLTLSKILRGRYFPTLSVFYASVKSSSSYFWKGFVLGLGFAEGGIRR 60
            + AK  WR + +PNL +SK+L+ +YF   S+  AS  S SSYFWKGF+ G      G+R 
Sbjct: 958  LVAKHVWRFLQHPNLLVSKVLKHKYFKDTSLLQASNNSKSSYFWKGFLWGRDLLVKGLRL 1017

Query: 61   NRANGQSIKIFKDPWIPHPVTFK----------------------WGVSKLNQFLCDEDV 109
               NG +IK F DPW+P P TFK                      W V+ ++   C+ED 
Sbjct: 1018 RVGNGSTIKAFSDPWLPRPTTFKPLRFNNGALDTTVASFITADGNWDVTSISHSFCNEDR 1077

BLAST of Lag0027730 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 8.2e-08
Identity = 27/76 (35.53%), Postives = 43/76 (56.58%), Query Frame = 0

Query: 1   MFAKQAWRVVVNPNLTLSKILRGRYFPTLSVFYASVKSSSSYFWKGFVLGLGFAEGGIRR 60
           + AKQ++R++  P+  LS++LR RYFP  S+   SV +  SY W+  + G      G+ R
Sbjct: 69  LLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRGLLR 128

Query: 61  NRANGQSIKIFKDPWI 77
              +G   K++ D WI
Sbjct: 129 TIGDGIHTKVWLDRWI 144

BLAST of Lag0027730 vs. ExPASy TrEMBL
Match: A0A803P4P2 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 4.3e-20
Identity = 85/294 (28.91%), Postives = 126/294 (42.86%), Query Frame = 0

Query: 1    MFAKQAWRVVVNPNLTLSKILRGRYFPTLSVFYASVKSSSSYFWKGFVLGLGFAEGGIRR 60
            M AKQAWRV+ NPN  L+ IL+ ++F   +   A +  S S+ W   + G      G+  
Sbjct: 1227 MIAKQAWRVLSNPNSLLAAILKAKHFKHTNFLEAKLGHSPSFTWSSLLWGRDLLNQGLMW 1286

Query: 61   NRANGQSIKIFKDPWIPHPVTFK------WGVSKLNQFLCDEDVRVISHLPI--NSSCSE 120
               NG SI+  ++ W+P     +      W  S L  F  +     I    I   S  S+
Sbjct: 1287 KVGNGCSIRTLENFWVPDSRFLRIGAIKIWKASPLKHFYANHRHVDIKEFLIAGYSHLSK 1346

Query: 121  PVLDLIYIGAWAIWNDRNYALHNRLVPSIIDRCVWIYRYLEDFRKINEVRASVSSSRV-- 180
              L L+    WAIWN RN  L      S +D   WI  YL D+         +SSS +  
Sbjct: 1347 EDLTLLLATVWAIWNFRNKKLFANCNMSSVDVVEWINSYLSDYNAAQVGMKKLSSSPILQ 1406

Query: 181  --DLHVLVDEGEETFMHTDAAFNEPNKKSEIGVVLRDKQGDLKAVLSSPSQVCISHLGAE 240
              +    V+ G    ++TDAA      K   G V+ D +G + A LS P+      L AE
Sbjct: 1407 DHEQPKQVNPGYYQ-LNTDAALCSSQGKLGFGAVISDWRGRIVAGLSIPAAGDFVPLMAE 1466

Query: 241  VVAVLEGIRSAISMGVESILVMCDSLSLVKALQGTNHCDSGISSVLWDTKGAFS 283
             +A+ E +     + +   ++  DS  LV  + G     S +S V+ D   + S
Sbjct: 1467 ALALRESLNWCYLIRIPIAVIKTDSKLLVDRIHGRKRDLSALSDVVEDISNSLS 1519

BLAST of Lag0027730 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 1.5e-17
Identity = 49/130 (37.69%), Postives = 68/130 (52.31%), Query Frame = 0

Query: 1    MFAKQAWRVVVNPNLTLSKILRGRYFPTLSVFYASVKSSSSYFWKGFVLGLGFAEGGIRR 60
            + AK  WR + +PNL +SK+L+ +YF   S+  AS  S SSYFWKGF+ G      G+R 
Sbjct: 958  LVAKHVWRFLQHPNLLVSKVLKHKYFKDTSLLQASNNSKSSYFWKGFLWGRDLLVKGLRL 1017

Query: 61   NRANGQSIKIFKDPWIPHPVTFK----------------------WGVSKLNQFLCDEDV 109
               NG +IK F DPW+P P TFK                      W V+ ++   C+ED 
Sbjct: 1018 RVGNGSTIKAFSDPWLPRPTTFKPLRFNNGALDTTVASFITADGNWDVTSISHSFCNEDR 1077

BLAST of Lag0027730 vs. ExPASy TrEMBL
Match: A0A803P5U5 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 5.9e-17
Identity = 81/342 (23.68%), Postives = 135/342 (39.47%), Query Frame = 0

Query: 1   MFAKQAWRVVVNPNLTLSKILRGRYFPTLSVFYASVKSSSSYFWKGFVLGLGFAEGGIRR 60
           + AKQAWR+V NPN  L K+L+ +YFP  S   ASV  S S  W+G   G      G+R 
Sbjct: 259 LLAKQAWRLVENPNSLLRKLLKSKYFPHNSFLQASVGHSPSLTWQGIRWGRELLIQGLRW 318

Query: 61  NRANGQSIKIFKDPWIPHPVTF---------------------KWGVSKLNQFLCDEDVR 120
             + G+SI+   DPWI    TF                     +W +S L Q+    DV 
Sbjct: 319 KISEGRSIRSGFDPWIHGHTTFTPIQYSGPRNGVVANLITDEKQWNLSLLQQYFSPLDVE 378

Query: 121 VISHLP----------------------------------INSSCSEPVLDLIYIGAWAI 180
            I  +P                                  ++S  ++  ++ ++   WAI
Sbjct: 379 KILTMPLSYFPTRDKLIWHHHSFGKFSVDASMKNGDYLQHLSSIYNKAEMEQLFCTLWAI 438

Query: 181 WNDRNYALHNRLVPSIIDRCVWIYRYLEDFRKINEVRAS---VSSSRVDLHVLVDEGEET 240
           W +RN  +H +     +D  ++   +L++++       S   V +S    HV       T
Sbjct: 439 WTERNKVVHGKNARCAVDLALYSMVFLQNYKNAQVKTTSAVNVEASGSSHHV----SPPT 498

Query: 241 FMHTDAAFNEPNKK-SEIGVVLRDKQGDLKAVLSSPSQVCISHLGAEVVAVLEGIRSAIS 284
             H     ++ +   + +G VLRD  G +KA LS P          E  ++   +  A+ 
Sbjct: 499 AAHVPWCPSDASTSITGVGAVLRDANGQVKAALSMPILRDFKSHEMEAKSLFHSLNWALQ 558

BLAST of Lag0027730 vs. ExPASy TrEMBL
Match: A0A803PZ52 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 5.0e-16
Identity = 83/286 (29.02%), Postives = 123/286 (43.01%), Query Frame = 0

Query: 1   MFAKQAWRVVVNPNLTLSKILRGRYFPTLSVFYASVKSSSSYFWKGFVLGLGFAEGGIRR 60
           M AKQAWRV+ NPN TL+ IL+ +YF     + A +  S S+ W   + G          
Sbjct: 315 MIAKQAWRVLSNPNSTLAAILKAKYFKHTGFYEARLGHSPSFTWSSLLWGRDLLSQVTHA 374

Query: 61  NRANGQSIKIFKDPWIPHPVTFKWGVSKLNQFLCDEDVRVISHLPINSSCSEPVLDLIYI 120
                 + KI+K   + H       V  + +FL    +   SHL      ++  L L+  
Sbjct: 375 LLDCSSAAKIWKASPLKHFYVTHRHVD-IKEFL----ISGYSHL------NKEDLTLLLT 434

Query: 121 GAWAIWNDRNYALHNRLVPSIIDRCVWIYRYLEDFRKINEVRASVSSSRVDLHVLVDEGE 180
             WAIWN RN  L   L  S  D   WI  YL D+         +S S++ LHV   + +
Sbjct: 435 TVWAIWNLRNKKLFANLNMSSADVIDWINSYLSDYNAAQLNMRKLSPSQI-LHVHGQQKQ 494

Query: 181 ET----FMHTDAAFNEPNKKSEIGVVLRDKQGDLKAVLSSPSQVCISHLGAEVVAVLEGI 240
                  ++TDAA      K   G V+ D  G + A LS PS   +  L AE +A+   +
Sbjct: 495 VNPGYYQLNTDAALCSSQGKLGFGAVINDWHGRIVAGLSIPSAGDLFPLMAEALALRASL 554

Query: 241 RSAISMGVESILVMCDSLSLVKALQGTNHCDSGISSVLWDTKGAFS 283
                + +   ++  DS  LV  + G     S +S V+ D + + S
Sbjct: 555 NWCYHIRILIAVIETDSKLLVDRIYGRKRDLSVLSDVVEDIRSSLS 588

BLAST of Lag0027730 vs. ExPASy TrEMBL
Match: A0A803NGQ8 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 6.5e-16
Identity = 80/314 (25.48%), Postives = 129/314 (41.08%), Query Frame = 0

Query: 1    MFAKQAWRVVVNPNLTLSKILRGRYFPTLSVFYASVKSSSSYFWKGFVLGLGFAEGGIRR 60
            + AKQAWR++ NP+  ++++L+ +YF       AS     SY W   + G      G+  
Sbjct: 1338 LLAKQAWRILTNPHSLIARLLKAKYFKHNDFLSASKGHCPSYTWSSLLWGRDLLSSGLIS 1397

Query: 61   NRANGQSIKIFKDPWIP--HPVTFK-------------------WGVSKLNQFLCDEDVR 120
               NG++    +  WIP    +T+K                   W V KLN +   + V+
Sbjct: 1398 KIGNGETTDTLRHYWIPGFKNITYKTHVPPPNPTVSFFISDSGSWDVHKLNTYFSQDIVK 1457

Query: 121  VISHLPINSS-------CSEP------VLDLIYIG---------------AWAIWNDRNY 180
             I  +PI+ S        S P      + D +  G                W+IWN RN 
Sbjct: 1458 AILTIPIDISQPDSLIWSSHPSGNLTDIKDFLLTGFEILPKDQLSLCVALLWSIWNKRNQ 1517

Query: 181  ALHNRLVPSIIDRCVWIYRYLEDFRKINEVRAS---VSSSRVDLHVLVDEGEETFMHTDA 240
             L     P + D    +  YL+++    +  A+   +  +      L   G  T +++DA
Sbjct: 1518 TLFQSKSPPLHDIEDSVVDYLKEYSNSQQHSAASIPIPPATGTQPPLPPAGMYT-LYSDA 1577

Query: 241  AFNEPNKKSEIGVVLRDKQGDLKAVLSSPSQVCISHLGAEVVAVLEGIR--SAISMGVES 261
            A + P      G  ++D  G + A LSSP    +S   AE  A+L+ I+  S + + + S
Sbjct: 1578 AISTPRSTMGFGAAIQDSTGQIIAALSSPHTGALSPTLAEAYALLQAIQWCSLVHLPLHS 1637

BLAST of Lag0027730 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 59.3 bits (142), Expect = 5.8e-09
Identity = 27/76 (35.53%), Postives = 43/76 (56.58%), Query Frame = 0

Query: 1   MFAKQAWRVVVNPNLTLSKILRGRYFPTLSVFYASVKSSSSYFWKGFVLGLGFAEGGIRR 60
           + AKQ++R++  P+  LS++LR RYFP  S+   SV +  SY W+  + G      G+ R
Sbjct: 69  LLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRGLLR 128

Query: 61  NRANGQSIKIFKDPWI 77
              +G   K++ D WI
Sbjct: 129 TIGDGIHTKVWLDRWI 144

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_030939737.12.0e-1926.13uncharacterized protein LOC115964598 [Quercus lobata][more]
XP_031099874.11.9e-1727.55uncharacterized protein LOC116004082 [Ipomoea triloba][more]
XP_024178198.11.9e-1723.28uncharacterized protein LOC112184155 [Rosa chinensis][more]
XP_042973059.13.2e-1723.94uncharacterized protein LOC122304861 [Carya illinoinensis][more]
XP_022158377.13.2e-1737.69uncharacterized protein LOC111024874 [Momordica charantia][more]
Match NameE-valueIdentityDescription
P932958.2e-0835.53Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A803P4P24.3e-2028.91Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A6J1DX301.5e-1737.69uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A803P5U55.9e-1723.68Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803PZ525.0e-1629.02Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803NGQ86.5e-1625.48Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
ATMG00310.15.8e-0935.53RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 182..280
e-value: 2.5E-8
score: 36.0
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 186..277
e-value: 1.0E-14
score: 54.4
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 182..266

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0027730.1Lag0027730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity