Lag0027708 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0027708
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Ulp1-like peptidase
Location	chr8: 3823985 .. 3825291 (+)
RNA-Seq Expression	Lag0027708
Synteny	Lag0027708

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTACCGGGATTTACAGGAGTTGGAGTCTCAACCAACCACTGACGTATCGTCTCACTCCATTACTGACGTGGAGTATCAACCAAGTACAGACCCAGTCGAGTTCATTGTACCTAAGGCTGAGGTATTTGTCTTAATATAAACAATATTATATAAATCTCACTAATGTATTAAATGTATGTTTGTAGCCTGTTGAGTTAAGTGATGAGGATGTTGAGGAGGTTGAGGAGGTTAAAGTTATTGGGCCGCATGAATTGGTAAAAAGACGGGGAAAGCGGACCTGACAAATTTCTTGGAAGCTTCGGTCTCCATGGGCTGACACCAGATCGGACGGCAAAAGGAGGAAAGTTAAGCGATACGATCCCATGCGTGCCATTCCTGAGGAGTACTAGACCAAGTTCCAGAAATGGTTGGATGACCCATTGTCTGATGGATCGGAGCGCAAGACAGTCTATGCATACAGAAGTAAACAATGGTTCAAGACGTTACTCACATCGTCTCATTGGATGGGTGATGAGGTACAACTCATTTTATCACTGCTGTTGATTTGTAAATGTTATGGTTTATGATTCTACAATTCACTATTCTTCTGATTCTATAGGTGATTGACTCTCTCTTCCTCTTTGTTCGAAAGAAGATGGATACCTGTCCTGACTTATGCCATCGAAAGTTCGTCACAGCTGATGTATTTGTAACATTACGTATTTTCATCTTTATAGGATGTCAATTAATCTGAATTGTACTAATATTGGATGTTGTGCAGGAATTTTGAGGCGCGAGGATGTGTACGAAGAACTCCTTCATAGTGACCATTGGAACGACACGTTCGATTGGAGTAGATTCAAGACGGTCACTAAATACATAAAGGGAGAACACACAGACTATAATGTTCATTGGAGTTCGGTTGATGTTGTCTACATGCCCTTCAACTTAGGTAGAAACCATTGGGTTCTACTGTGCGCTGACTTTGAAATGGACGAATTTGTGTTGAGAGACTCCCTAACGGCACTGAATTCAGATGCAGACATAGCGAAGCAGATGAATACGGTATGCACCATTTTCCCTAGGCTGCTACTGAGGTGCGACGTTATGAAGGAGAAGCCATCTCTTCCAAGACATCCATGGCGATTCAGAAGGAAGACCCAAGTGCCACAACAACAAGATAGTGGAGATTGTGGGGCTTTCACTGTAAAGTTTTTAGAATATGATGTAACTAAATCAGATTTAGGTAGTCTTAGTCAAGAGAAAATGGAGTTTTGTAGGCGTCAATTTGCTGTACAACTTTGGGCCAATAGGTCGTTCTTTTAG

mRNA sequence

ATGTACCGGGATTTACAGGAGTTGGAGTCTCAACCAACCACTGACGTATCGTCTCACTCCATTACTGACGTGGAGTATCAACCAAGTACAGACCCAGTCGAGTTCATTGTACCTAAGGCTGAGCCTGTTGAGTTAAGTGATGAGGATGTTGAGGAGGTTGAGGAGGTTAAAGTTATTGGGCCGCATGAATTGACCAAGTTCCAGAAATGGTTGGATGACCCATTGTCTGATGGATCGGAGCGCAAGACAGTCTATGCATACAGAAGTAAACAATGGTTCAAGACGTTACTCACATCGTCTCATTGGATGGGTGATGAGGTGATTGACTCTCTCTTCCTCTTTGTTCGAAAGAAGATGGATACCTGTCCTGACTTATGCCATCGAAAGTTCGTCACAGCTGATGTATTTGTAACATTACGAATTTTGAGGCGCGAGGATGTGTACGAAGAACTCCTTCATAGTGACCATTGGAACGACACGTTCGATTGGAGTAGATTCAAGACGGTCACTAAATACATAAAGGGAGAACACACAGACTATAATGTTCATTGGAGTTCGGTTGATGTTGTCTACATGCCCTTCAACTTAGGTAGAAACCATTGGGTTCTACTGTGCGCTGACTTTGAAATGGACGAATTTGTGTTGAGAGACTCCCTAACGGCACTGAATTCAGATGCAGACATAGCGAAGCAGATGAATACGGTATGCACCATTTTCCCTAGGCTGCTACTGAGGTGCGACGTTATGAAGGAGAAGCCATCTCTTCCAAGACATCCATGGCGATTCAGAAGGAAGACCCAAGTGCCACAACAACAAGATAGTGGAGATTGTGGGGCTTTCACTGTAAAGTTTTTAGAATATGATGTAACTAAATCAGATTTAGGTAGTCTTAGTCAAGAGAAAATGGAGTTTTGTAGGCGTCAATTTGCTGTACAACTTTGGGCCAATAGGTCGTTCTTTTAG

Coding sequence (CDS)

ATGTACCGGGATTTACAGGAGTTGGAGTCTCAACCAACCACTGACGTATCGTCTCACTCCATTACTGACGTGGAGTATCAACCAAGTACAGACCCAGTCGAGTTCATTGTACCTAAGGCTGAGCCTGTTGAGTTAAGTGATGAGGATGTTGAGGAGGTTGAGGAGGTTAAAGTTATTGGGCCGCATGAATTGACCAAGTTCCAGAAATGGTTGGATGACCCATTGTCTGATGGATCGGAGCGCAAGACAGTCTATGCATACAGAAGTAAACAATGGTTCAAGACGTTACTCACATCGTCTCATTGGATGGGTGATGAGGTGATTGACTCTCTCTTCCTCTTTGTTCGAAAGAAGATGGATACCTGTCCTGACTTATGCCATCGAAAGTTCGTCACAGCTGATGTATTTGTAACATTACGAATTTTGAGGCGCGAGGATGTGTACGAAGAACTCCTTCATAGTGACCATTGGAACGACACGTTCGATTGGAGTAGATTCAAGACGGTCACTAAATACATAAAGGGAGAACACACAGACTATAATGTTCATTGGAGTTCGGTTGATGTTGTCTACATGCCCTTCAACTTAGGTAGAAACCATTGGGTTCTACTGTGCGCTGACTTTGAAATGGACGAATTTGTGTTGAGAGACTCCCTAACGGCACTGAATTCAGATGCAGACATAGCGAAGCAGATGAATACGGTATGCACCATTTTCCCTAGGCTGCTACTGAGGTGCGACGTTATGAAGGAGAAGCCATCTCTTCCAAGACATCCATGGCGATTCAGAAGGAAGACCCAAGTGCCACAACAACAAGATAGTGGAGATTGTGGGGCTTTCACTGTAAAGTTTTTAGAATATGATGTAACTAAATCAGATTTAGGTAGTCTTAGTCAAGAGAAAATGGAGTTTTGTAGGCGTCAATTTGCTGTACAACTTTGGGCCAATAGGTCGTTCTTTTAG

Protein sequence

MYRDLQELESQPTTDVSSHSITDVEYQPSTDPVEFIVPKAEPVELSDEDVEEVEEVKVIGPHELTKFQKWLDDPLSDGSERKTVYAYRSKQWFKTLLTSSHWMGDEVIDSLFLFVRKKMDTCPDLCHRKFVTADVFVTLRILRREDVYEELLHSDHWNDTFDWSRFKTVTKYIKGEHTDYNVHWSSVDVVYMPFNLGRNHWVLLCADFEMDEFVLRDSLTALNSDADIAKQMNTVCTIFPRLLLRCDVMKEKPSLPRHPWRFRRKTQVPQQQDSGDCGAFTVKFLEYDVTKSDLGSLSQEKMEFCRRQFAVQLWANRSFF

Homology

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BLAST of Lag0027708 vs. NCBI nr
Match: XP_022158807.1 (uncharacterized protein LOC111025273 [Momordica charantia])

HSP 1 Score: 184.5 bits (467), Expect = 1.4e-42
Identity = 96/255 (37.65%), Postives = 140/255 (54.90%), Query Frame = 0


Query: 71  LDDPLSDGSERKTVYAYRSKQWFKTLLTSSHWMGDEVIDSLFLFVRKKMDTCPDLCHRKF 130
           +DDP +D + R T    + K WF  LL     + DE IDSL +   +K++ C  L   +F
Sbjct: 1   MDDPSTDDNSRSTSVGIKYKSWFGLLLDPQFQLDDEYIDSLMMLTARKVEKCKHLLRVQF 60

Query: 131 VTADVFVTLRILRRED-----VYEELLHSDHWNDTFDWSRFKTVTKYIKGEHTDYNVHWS 190
              DV ++  +LRR D     +   +L S     T+DW + +T+ +Y+ G  +DY+  WS
Sbjct: 61  AIGDVLLS-NLLRRTDGPYAAMKPGVLPS---KCTYDWRQERTIFRYVLGRQSDYDTLWS 120

Query: 191 SVDVVYMPFNLGRNHWVLLCADFEMDEFVLRDSLTALNSDADIAKQMNTVCTIFPRLLLR 250
             D+VY   N+G NHWV++  D    +  + DSL A+    D+ K +  +CTI P +L  
Sbjct: 121 EADIVYTRMNIGGNHWVMIGIDLVEGDLTVWDSLQAITPLEDLEKALKPMCTIIPAILHW 180

Query: 251 CDVMKEKPSLPRHPWRFRRKTQVPQQQDSGDCGAFTVKFLEYDVTKSDLGSLSQEKMEFC 310
             ++  +P+LP  PWR RR T VPQQ    DC  F V+F EYDV  S + +L Q  +   
Sbjct: 181 SGILALRPNLPMVPWRVRRCT-VPQQAGFTDCSIFCVRFFEYDVIGSKIDTLIQSNISLF 240

Query: 311 RRQFAVQLWANRSFF 321
           RRQ+AVQ+WA R FF
Sbjct: 241 RRQYAVQMWARRPFF 250

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BLAST of Lag0027708 vs. NCBI nr
Match: XP_038882332.1 (uncharacterized protein LOC120073583 [Benincasa hispida])

HSP 1 Score: 177.6 bits (449), Expect = 1.8e-40
Identity = 85/161 (52.80%), Postives = 112/161 (69.57%), Query Frame = 0


Query: 160 TFDWSRFKTVTKYIKGEHTDYNVHWSSVDVVYMPFNLGRNHWVLLCADFEMDEFVLRDSL 219
           T DWS  K V KY+ G+HTDY+V WS+VD VYMPFNL   HWVL+CADF++ E ++ DSL
Sbjct: 59  TIDWSMEKKVLKYVHGKHTDYDVPWSNVDAVYMPFNLSGMHWVLVCADFQVRELIMFDSL 118

Query: 220 TALNSDADIAKQMNTVCTIFPRLLLRCDVMKEKPSLPRHPWRFRRKTQVPQQQDSGDCGA 279
            AL+ +AD+  +M  VC  FP LL+   VM E  +L    W  RR     QQ +SGDCG 
Sbjct: 119 IALHLNADLECEMRLVCKNFPGLLIVGAVM-ESHNLLIDRWTLRRDAFRSQQHESGDCGM 178

Query: 280 FTVKFLEYDVTKSDLGSLSQEKMEFCRRQFAVQLWANRSFF 321
           FT KF EYDVT S +G+L+Q++ ++ RRQ+A+Q+WANR+ F
Sbjct: 179 FTYKFFEYDVTGSKMGTLTQDRKQYFRRQYAIQIWANRALF 218

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BLAST of Lag0027708 vs. NCBI nr
Match: XP_038885861.1 (sentrin-specific protease [Benincasa hispida])

HSP 1 Score: 173.3 bits (438), Expect = 3.3e-39
Identity = 81/161 (50.31%), Postives = 111/161 (68.94%), Query Frame = 0


Query: 160 TFDWSRFKTVTKYIKGEHTDYNVHWSSVDVVYMPFNLGRNHWVLLCADFEMDEFVLRDSL 219
           T DWS+   V KY+ G+HTDY+V WS+VD +YMPFNL R HWVL+C DF++ E ++ DSL
Sbjct: 30  TIDWSKTTNVVKYVHGKHTDYDVPWSNVDAIYMPFNLSRMHWVLVCVDFQVRELIVFDSL 89

Query: 220 TALNSDADIAKQMNTVCTIFPRLLLRCDVMKEKPSLPRHPWRFRRKTQVPQQQDSGDCGA 279
             L+ +AD+  +M ++C  F  LL+   VM E  +L    W  RR   VPQQ  SGDCG 
Sbjct: 90  IVLHLNADLEHEMRSLCKNFLGLLIASAVM-ESHNLLIDRWTLRRDAFVPQQDKSGDCGM 149

Query: 280 FTVKFLEYDVTKSDLGSLSQEKMEFCRRQFAVQLWANRSFF 321
           FT KF EYDVT S + +L+Q++M++ RRQ+A+Q+ ANR+ F
Sbjct: 150 FTCKFFEYDVTGSKMDTLTQDRMQYYRRQYAIQISANRTLF 189

[Close]

BLAST of Lag0027708 vs. NCBI nr
Match: XP_022154364.1 (uncharacterized protein LOC111021646 [Momordica charantia])

HSP 1 Score: 172.6 bits (436), Expect = 5.7e-39
Identity = 82/211 (38.86%), Postives = 121/211 (57.35%), Query Frame = 0


Query: 113 LFVRKKMDTCPDLCHRKFVTADVFVTLRILRREDVYEEL----LHSDHWNDTFDW-SRFK 172
           +FV  K+   P+LC RKF T DV ++  +   + VY  +    + +      +DW  R  
Sbjct: 1   MFVCNKLKLRPNLCRRKFTTGDVLISNFLRSTDGVYVMMQSPNVIASRVASDYDWEGRAW 60

Query: 173 TVTKYIKGEHTDYNVHWSSVDVVYMPFNLGRNHWVLLCADFEMDEFVLRDSLTALNSDAD 232
           ++  YI G H+D +  W  VD VY+P+N+G  HW+++C DF+  E ++ DS   +     
Sbjct: 61  SMLSYIDGTHSDNDTRWMDVDAVYLPYNIGGVHWIVICIDFDEGELIVWDSFMNMTPLPQ 120

Query: 233 IAKQMNTVCTIFPRLLLRCDVMKEKPSLPRHPWRFRRKTQVPQQQDSGDCGAFTVKFLEY 292
           + +++  + TI P L+ R  V   KP++P  PWR RR +  PQQ   GDCG F + F EY
Sbjct: 121 LEQELKPMITIIPTLICRVGVHLYKPNIPLTPWRIRRVSSAPQQGMDGDCGIFCINFFEY 180

Query: 293 DVTKSDLGSLSQEKMEFCRRQFAVQLWANRS 319
           DVT     +L+Q +M F RRQFAVQLWAN+S
Sbjct: 181 DVTSCSFDTLTQSRMSFFRRQFAVQLWANKS 211

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BLAST of Lag0027708 vs. NCBI nr
Match: KAA0044249.1 (Ulp1-like peptidase [Cucumis melo var. makuwa])

HSP 1 Score: 164.1 bits (414), Expect = 2.0e-36
Identity = 85/266 (31.95%), Postives = 136/266 (51.13%), Query Frame = 0


Query: 57  KVIGPHELTKFQKWLDDPLSDGSERKTVYAYRSKQWFKTLLTSSHWMGDEVIDSLFLFVR 116
           K++  H L + + W+ D  +D   R+T +  +SK +F+ L     W+ DE +D+LFLF+R
Sbjct: 446 KILDVH-LDRLRAWITDKRTDDELRETFHGKKSKAFFRDLFMCRRWLSDEHLDALFLFIR 505

Query: 117 KKMDTCPDLCHRKFVTADVFVTLRILRREDVYEELLHSDHWNDTFDWSRFKTVTKYIKGE 176
            K+        + F TAD      ++ +  +Y+E +     N  FDW     +  Y+ G 
Sbjct: 506 LKIKAAGIPSSQNFTTADTIFMRILVSKWPLYKECIKE---NRPFDWDEEYRLVDYVVGS 565

Query: 177 HTDYNVHWSSVDVVYMPFNLGRNHWVLLCADFEMDEFVLRDSLTALNSDADIAKQMNTVC 236
             D+   W+SVD VY PFN+  NHWVLLC D    +  + DSL +L +  ++   +  + 
Sbjct: 566 KVDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQVKVWDSLPSLTTAEEMTNILLPIR 625

Query: 237 TIFPRLLLRCDVM--KEKPSLPRHPWRFRRKTQVPQQQDSGDCGAFTVKFLEYDVTKSDL 296
            + P+LL        + + S  + PW       +P Q+++ DCG FT+K+ EY  T   L
Sbjct: 626 QLVPKLLDSTGFFDRRGRSSTYKEPWPVVIVDSIPLQRNNSDCGVFTIKYFEYIATGVGL 685

Query: 297 GSLSQEKMEFCRRQFAVQLWANRSFF 321
            +L QE M + R+Q A QLW N   +
Sbjct: 686 DTLCQENMSYFRKQLAFQLWTNTPMY 707

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BLAST of Lag0027708 vs. ExPASy Swiss-Prot
Match: Q94F30 (Ubiquitin-like-specific protease ESD4 OS=Arabidopsis thaliana OX=3702 GN=ESD4 PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 2.1e-04
Identity = 50/219 (22.83%), Postives = 92/219 (42.01%), Query Frame = 0


Query: 97  LTSSHWMGDEVIDSLFLFVRKKMDTCPDLCHRKFVTADVFVTLRILRREDVYEELLHSDH 156
           LT S W+ DEVI +++L + K+ +T      +K++    F T         Y++L+    
Sbjct: 298 LTPSAWLNDEVI-NVYLELLKERETREP---KKYLKCHYFNTF-------FYKKLVSDSG 357

Query: 157 WNDTFDWSRFKTVTKYIKGEHTDYNVHWSSVDVVYMPFNLGRNHWVLLCADFEMDEFVLR 216
           +N       FK V ++       Y +     D++++P + G  HW L   +    + +  
Sbjct: 358 YN-------FKAVRRWTTQRKLGYAL--IDCDMIFVPIHRG-VHWTLAVINNRESKLLYL 417

Query: 217 DSLTALNSDA--DIAKQMNTVCTIFPRLLLRCDVMKEKPSLPRHPWRFRRKTQVPQQQDS 276
           DSL  ++      +AK M                 K    +  + W       +PQQ++ 
Sbjct: 418 DSLNGVDPMILNALAKYMGDEAN-----------EKSGKKIDANSWDMEFVEDLPQQKNG 477

Query: 277 GDCGAFTVKFLEYDVTKSDLGSLSQEKMEFCRRQFAVQL 314
            DCG F +K++++      L   SQE M + R + A ++
Sbjct: 478 YDCGMFMLKYIDFFSRGLGL-CFSQEHMPYFRLRTAKEI 483

[Close]

BLAST of Lag0027708 vs. ExPASy TrEMBL
Match: A0A6J1DY60 (uncharacterized protein LOC111025273 OS=Momordica charantia OX=3673 GN=LOC111025273 PE=3 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 7.0e-43
Identity = 96/255 (37.65%), Postives = 140/255 (54.90%), Query Frame = 0


Query: 71  LDDPLSDGSERKTVYAYRSKQWFKTLLTSSHWMGDEVIDSLFLFVRKKMDTCPDLCHRKF 130
           +DDP +D + R T    + K WF  LL     + DE IDSL +   +K++ C  L   +F
Sbjct: 1   MDDPSTDDNSRSTSVGIKYKSWFGLLLDPQFQLDDEYIDSLMMLTARKVEKCKHLLRVQF 60

Query: 131 VTADVFVTLRILRRED-----VYEELLHSDHWNDTFDWSRFKTVTKYIKGEHTDYNVHWS 190
              DV ++  +LRR D     +   +L S     T+DW + +T+ +Y+ G  +DY+  WS
Sbjct: 61  AIGDVLLS-NLLRRTDGPYAAMKPGVLPS---KCTYDWRQERTIFRYVLGRQSDYDTLWS 120

Query: 191 SVDVVYMPFNLGRNHWVLLCADFEMDEFVLRDSLTALNSDADIAKQMNTVCTIFPRLLLR 250
             D+VY   N+G NHWV++  D    +  + DSL A+    D+ K +  +CTI P +L  
Sbjct: 121 EADIVYTRMNIGGNHWVMIGIDLVEGDLTVWDSLQAITPLEDLEKALKPMCTIIPAILHW 180

Query: 251 CDVMKEKPSLPRHPWRFRRKTQVPQQQDSGDCGAFTVKFLEYDVTKSDLGSLSQEKMEFC 310
             ++  +P+LP  PWR RR T VPQQ    DC  F V+F EYDV  S + +L Q  +   
Sbjct: 181 SGILALRPNLPMVPWRVRRCT-VPQQAGFTDCSIFCVRFFEYDVIGSKIDTLIQSNISLF 240

Query: 311 RRQFAVQLWANRSFF 321
           RRQ+AVQ+WA R FF
Sbjct: 241 RRQYAVQMWARRPFF 250

[Close]

BLAST of Lag0027708 vs. ExPASy TrEMBL
Match: A0A6J1DLV0 (uncharacterized protein LOC111021646 OS=Momordica charantia OX=3673 GN=LOC111021646 PE=3 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 2.7e-39
Identity = 82/211 (38.86%), Postives = 121/211 (57.35%), Query Frame = 0


Query: 113 LFVRKKMDTCPDLCHRKFVTADVFVTLRILRREDVYEEL----LHSDHWNDTFDW-SRFK 172
           +FV  K+   P+LC RKF T DV ++  +   + VY  +    + +      +DW  R  
Sbjct: 1   MFVCNKLKLRPNLCRRKFTTGDVLISNFLRSTDGVYVMMQSPNVIASRVASDYDWEGRAW 60

Query: 173 TVTKYIKGEHTDYNVHWSSVDVVYMPFNLGRNHWVLLCADFEMDEFVLRDSLTALNSDAD 232
           ++  YI G H+D +  W  VD VY+P+N+G  HW+++C DF+  E ++ DS   +     
Sbjct: 61  SMLSYIDGTHSDNDTRWMDVDAVYLPYNIGGVHWIVICIDFDEGELIVWDSFMNMTPLPQ 120

Query: 233 IAKQMNTVCTIFPRLLLRCDVMKEKPSLPRHPWRFRRKTQVPQQQDSGDCGAFTVKFLEY 292
           + +++  + TI P L+ R  V   KP++P  PWR RR +  PQQ   GDCG F + F EY
Sbjct: 121 LEQELKPMITIIPTLICRVGVHLYKPNIPLTPWRIRRVSSAPQQGMDGDCGIFCINFFEY 180

Query: 293 DVTKSDLGSLSQEKMEFCRRQFAVQLWANRS 319
           DVT     +L+Q +M F RRQFAVQLWAN+S
Sbjct: 181 DVTSCSFDTLTQSRMSFFRRQFAVQLWANKS 211

[Close]

BLAST of Lag0027708 vs. ExPASy TrEMBL
Match: A0A5A7TSP7 (Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2741G00060 PE=3 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 9.7e-37
Identity = 85/266 (31.95%), Postives = 136/266 (51.13%), Query Frame = 0


Query: 57  KVIGPHELTKFQKWLDDPLSDGSERKTVYAYRSKQWFKTLLTSSHWMGDEVIDSLFLFVR 116
           K++  H L + + W+ D  +D   R+T +  +SK +F+ L     W+ DE +D+LFLF+R
Sbjct: 446 KILDVH-LDRLRAWITDKRTDDELRETFHGKKSKAFFRDLFMCRRWLSDEHLDALFLFIR 505

Query: 117 KKMDTCPDLCHRKFVTADVFVTLRILRREDVYEELLHSDHWNDTFDWSRFKTVTKYIKGE 176
            K+        + F TAD      ++ +  +Y+E +     N  FDW     +  Y+ G 
Sbjct: 506 LKIKAAGIPSSQNFTTADTIFMRILVSKWPLYKECIKE---NRPFDWDEEYRLVDYVVGS 565

Query: 177 HTDYNVHWSSVDVVYMPFNLGRNHWVLLCADFEMDEFVLRDSLTALNSDADIAKQMNTVC 236
             D+   W+SVD VY PFN+  NHWVLLC D    +  + DSL +L +  ++   +  + 
Sbjct: 566 KVDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQVKVWDSLPSLTTAEEMTNILLPIR 625

Query: 237 TIFPRLLLRCDVM--KEKPSLPRHPWRFRRKTQVPQQQDSGDCGAFTVKFLEYDVTKSDL 296
            + P+LL        + + S  + PW       +P Q+++ DCG FT+K+ EY  T   L
Sbjct: 626 QLVPKLLDSTGFFDRRGRSSTYKEPWPVVIVDSIPLQRNNSDCGVFTIKYFEYIATGVGL 685

Query: 297 GSLSQEKMEFCRRQFAVQLWANRSFF 321
            +L QE M + R+Q A QLW N   +
Sbjct: 686 DTLCQENMSYFRKQLAFQLWTNTPMY 707

[Close]

BLAST of Lag0027708 vs. ExPASy TrEMBL
Match: A0A5A7TD74 (Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold125G00310 PE=3 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 2.2e-36
Identity = 85/266 (31.95%), Postives = 136/266 (51.13%), Query Frame = 0


Query: 57  KVIGPHELTKFQKWLDDPLSDGSERKTVYAYRSKQWFKTLLTSSHWMGDEVIDSLFLFVR 116
           K++  H L + + W+ D  +D   R+T +  +SK +F+ L     W+ DE +D+LFLF+R
Sbjct: 293 KILDVH-LDRLRAWITDKRTDDEVRETFHGKKSKVFFRDLFMCRQWLADEHLDALFLFIR 352

Query: 117 KKMDTCPDLCHRKFVTADVFVTLRILRREDVYEELLHSDHWNDTFDWSRFKTVTKYIKGE 176
            K+        + F TAD   T  ++ +  +Y+E +     N  FDW     +  Y+ G 
Sbjct: 353 LKIKAVGIPSSQNFTTADTIFTGILVAKWPLYKECIKE---NRPFDWEEEYMLVDYVVGS 412

Query: 177 HTDYNVHWSSVDVVYMPFNLGRNHWVLLCADFEMDEFVLRDSLTALNSDADIAKQMNTVC 236
             D+   W+SVD VY PFN+  NHWVLLC D    +  + DSL +L +  ++   +  + 
Sbjct: 413 KEDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQVKVWDSLPSLTTAEEMTNILLPIR 472

Query: 237 TIFPRLLLRCDVM--KEKPSLPRHPWRFRRKTQVPQQQDSGDCGAFTVKFLEYDVTKSDL 296
            + P+LL        + + S  + PW       +P Q+++ DCG FT+K+ EY      L
Sbjct: 473 ELVPKLLDSTGFFDTRGRSSTYKEPWPVVIVDSIPLQRNNSDCGVFTIKYFEYIAAGVGL 532

Query: 297 GSLSQEKMEFCRRQFAVQLWANRSFF 321
            +L QE M + R+Q A QLW N   +
Sbjct: 533 DTLCQENMSYFRKQLAFQLWTNTPMY 554

[Close]

BLAST of Lag0027708 vs. ExPASy TrEMBL
Match: A0A5A7TVI1 (Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold115G00120 PE=3 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 3.7e-36
Identity = 84/266 (31.58%), Postives = 135/266 (50.75%), Query Frame = 0


Query: 57  KVIGPHELTKFQKWLDDPLSDGSERKTVYAYRSKQWFKTLLTSSHWMGDEVIDSLFLFVR 116
           K++  H L + + W+ D  +D   R+T +  +SK +F+ L     W+ DE +D+LFLF+ 
Sbjct: 487 KILDVH-LDRLRAWITDKRTDDELRETFHGKKSKAFFRDLFMCRRWLADEHLDALFLFIC 546

Query: 117 KKMDTCPDLCHRKFVTADVFVTLRILRREDVYEELLHSDHWNDTFDWSRFKTVTKYIKGE 176
            K+        + F TAD      ++ +  +Y+E +     N  FDW     +  Y+ G 
Sbjct: 547 LKIKAAGIPSSQNFTTADTIFMRILVSKWPLYKECIKE---NRPFDWDEEYRLVDYVVGS 606

Query: 177 HTDYNVHWSSVDVVYMPFNLGRNHWVLLCADFEMDEFVLRDSLTALNSDADIAKQMNTVC 236
             D+   W+SVD VY PFN+  NHWVLLC D    +  + DSL +L +  ++   +  + 
Sbjct: 607 KEDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQVKVWDSLPSLTTAEEMTNILLPIR 666

Query: 237 TIFPRLLLRCDVM--KEKPSLPRHPWRFRRKTQVPQQQDSGDCGAFTVKFLEYDVTKSDL 296
            + P+LL        + + S  + PW       +P Q+++ DCG F +K+ EY V    L
Sbjct: 667 QLVPKLLYSTGFFDRRGRSSTYKEPWPVVIVDSIPHQRNNSDCGVFIIKYFEYIVAGVGL 726

Query: 297 GSLSQEKMEFCRRQFAVQLWANRSFF 321
            +L QE M + R+QFA QLW N   +
Sbjct: 727 DTLCQENMSYFRKQFAFQLWTNTPMY 748

[Close]

BLAST of Lag0027708 vs. TAIR 10
Match: AT5G45570.1 (Ulp1 protease family protein )

HSP 1 Score: 62.4 bits (150), Expect = 7.7e-10
Identity = 37/129 (28.68%), Postives = 64/129 (49.61%), Query Frame = 0


Query: 187 VDVVYMPFNLGRNHWVLLCADFEMDEFVLRDSLTALNSDADIAKQMNTVCTIFPRLLLRC 246
           VD +Y    +  NHWV L  D       + DS+ +L +D ++A Q   V T+ P +L   
Sbjct: 768 VDHLYAYLFVNGNHWVALDIDLTNKRVNVYDSIPSLTTDTEMAIQCMFVMTMIPAMLSSF 827

Query: 247 DVMKE-KPSLPRHPWRFRRKTQVPQQQDSGDCGAFTVKFLEYDVTKSDLGSLSQEKMEFC 306
              K+ + S  +  W  +R T++P+  D GDC  +++K++E          L  E M+  
Sbjct: 828 IPSKQRRRSYSKLEW--KRITKIPENLDPGDCAIYSIKYIECLALGKSFDGLCDENMQSL 887

Query: 307 RRQFAVQLW 315
           R + AV+++
Sbjct: 888 RTKLAVEMF 894

[Close]

BLAST of Lag0027708 vs. TAIR 10
Match: AT4G08430.1 (Ulp1 protease family protein )

HSP 1 Score: 55.8 bits (133), Expect = 7.2e-08
Identity = 34/129 (26.36%), Postives = 62/129 (48.06%), Query Frame = 0


Query: 187 VDVVYMPFNLGRNHWVLLCADFEMDEFVLRDSLTALNSDADIAKQMNTVCTIFPRLLLRC 246
           VD +Y    +  NHWV L  D       + DS+ +L +D ++  Q   V T+ P +L   
Sbjct: 655 VDHLYAYLFVNGNHWVALDIDLPKKRINVYDSIPSLTTDTEMVIQCMFVMTMIPAMLSSF 714

Query: 247 DVMKE-KPSLPRHPWRFRRKTQVPQQQDSGDCGAFTVKFLEYDVTKSDLGSLSQEKMEFC 306
              K+ + S  +  W  +R T++P+  D+ DC  +++K++E          L  E M+  
Sbjct: 715 IPSKQRRRSYSKLEW--KRITKIPENLDACDCAIYSIKYIECLALGKSFDGLCDENMQSL 774

Query: 307 RRQFAVQLW 315
             + AV+++
Sbjct: 775 WTKLAVEMF 781

[Close]

BLAST of Lag0027708 vs. TAIR 10
Match: AT2G07240.1 (cysteine-type peptidases;cysteine-type peptidases )

HSP 1 Score: 53.5 bits (127), Expect = 3.6e-07
Identity = 29/99 (29.29%), Postives = 44/99 (44.44%), Query Frame = 0


Query: 184 WSSVDVVYMPFNLGRNHWVLLCADFEMDEFVLRDSLTALNSDADIAKQMNTVCTIFPRLL 243
           ++  D VYMPFN  + HWV LC D +  +  + DS   L  DA +  ++  +  + P L 
Sbjct: 738 FAEADCVYMPFNFDKKHWVSLCVDLKAHKITILDSNIQLRRDAALYAELQPLAAMLPYLF 797

Query: 244 LRCDVMKEKPSLPRHPWRFRRKTQVPQQQDSGDCGAFTV 283
            +        SL   P+   R   +PQ     D G  +V
Sbjct: 798 RQTSSSSGDISL--QPFLLDRPHGIPQVSSPFDSGVLSV 834

[Close]

BLAST of Lag0027708 vs. TAIR 10
Match: AT4G15880.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 48.1 bits (113), Expect = 1.5e-05
Identity = 50/219 (22.83%), Postives = 92/219 (42.01%), Query Frame = 0


Query: 97  LTSSHWMGDEVIDSLFLFVRKKMDTCPDLCHRKFVTADVFVTLRILRREDVYEELLHSDH 156
           LT S W+ DEVI +++L + K+ +T      +K++    F T         Y++L+    
Sbjct: 298 LTPSAWLNDEVI-NVYLELLKERETREP---KKYLKCHYFNTF-------FYKKLVSDSG 357

Query: 157 WNDTFDWSRFKTVTKYIKGEHTDYNVHWSSVDVVYMPFNLGRNHWVLLCADFEMDEFVLR 216
           +N       FK V ++       Y +     D++++P + G  HW L   +    + +  
Sbjct: 358 YN-------FKAVRRWTTQRKLGYAL--IDCDMIFVPIHRG-VHWTLAVINNRESKLLYL 417

Query: 217 DSLTALNSDA--DIAKQMNTVCTIFPRLLLRCDVMKEKPSLPRHPWRFRRKTQVPQQQDS 276
           DSL  ++      +AK M                 K    +  + W       +PQQ++ 
Sbjct: 418 DSLNGVDPMILNALAKYMGDEAN-----------EKSGKKIDANSWDMEFVEDLPQQKNG 477

Query: 277 GDCGAFTVKFLEYDVTKSDLGSLSQEKMEFCRRQFAVQL 314
            DCG F +K++++      L   SQE M + R + A ++
Sbjct: 478 YDCGMFMLKYIDFFSRGLGL-CFSQEHMPYFRLRTAKEI 483

[Close]

BLAST of Lag0027708 vs. TAIR 10
Match: AT3G06910.1 (UB-like protease 1A )

HSP 1 Score: 43.1 bits (100), Expect = 4.8e-04
Identity = 46/222 (20.72%), Postives = 90/222 (40.54%), Query Frame = 0


Query: 97  LTSSHWMGDEVIDSLFLFVRKKMDTCPDLCHRKFVTADVFVTLRILRREDVYEELLHSD- 156
           L    W+ DEVI+   + ++++    P    +KF+    F T         + +L++S  
Sbjct: 310 LKPGKWLNDEVINLYMVLLKEREAREP----KKFLKCHFFNTF-------FFTKLVNSAT 369

Query: 157 --HWNDTFDWSRFKTVTKYIKGEHTDYNVHWSSVDVVYMPFNLGRNHWVLLCADFEMDEF 216
             ++     W+  K +             H    D +++P ++   HW L   + +  +F
Sbjct: 370 GYNYGAVRRWTSMKRL-----------GYHLKDCDKIFIPIHM-NIHWTLAVINIKDQKF 429

Query: 217 VLRDSLTALNSDADIAKQMNTVCTIFPRLLLRCDVMKEKP--SLPRHPWRFRRKTQVPQQ 276
              DS           K ++ +   F       D +++K    L    WR      +P Q
Sbjct: 430 QYLDSFKGRE-----PKILDALARYF------VDEVRDKSEVDLDVSRWRQEFVQDLPMQ 489

Query: 277 QDSGDCGAFTVKFLEYDVTKSDLGSLSQEKMEFCRRQFAVQL 314
           ++  DCG F VK++++     DL   +QE+M + R + A ++
Sbjct: 490 RNGFDCGMFMVKYIDFYSRGLDL-CFTQEQMPYFRARTAKEI 496

The following BLAST results are available for this feature:

BLAST of Lag0027708 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158807.1	1.4e-42	37.65	uncharacterized protein LOC111025273 [Momordica charantia]	[more]
XP_038882332.1	1.8e-40	52.80	uncharacterized protein LOC120073583 [Benincasa hispida]	[more]
XP_038885861.1	3.3e-39	50.31	sentrin-specific protease [Benincasa hispida]	[more]
XP_022154364.1	5.7e-39	38.86	uncharacterized protein LOC111021646 [Momordica charantia]	[more]
KAA0044249.1	2.0e-36	31.95	Ulp1-like peptidase [Cucumis melo var. makuwa]	[more]

BLAST of Lag0027708 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94F30	2.1e-04	22.83	Ubiquitin-like-specific protease ESD4 OS=Arabidopsis thaliana OX=3702 GN=ESD4 PE...	[more]

BLAST of Lag0027708 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DY60	7.0e-43	37.65	uncharacterized protein LOC111025273 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6J1DLV0	2.7e-39	38.86	uncharacterized protein LOC111021646 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
A0A5A7TSP7	9.7e-37	31.95	Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2741...	[more]
A0A5A7TD74	2.2e-36	31.95	Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold125G...	[more]
A0A5A7TVI1	3.7e-36	31.58	Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold115G...	[more]

BLAST of Lag0027708 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45570.1	7.7e-10	28.68	Ulp1 protease family protein	[more]
AT4G08430.1	7.2e-08	26.36	Ulp1 protease family protein	[more]
AT2G07240.1	3.6e-07	29.29	cysteine-type peptidases;cysteine-type peptidases	[more]
AT4G15880.1	1.5e-05	22.83	Cysteine proteinases superfamily protein	[more]
AT3G06910.1	4.8e-04	20.72	UB-like protease 1A	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PFAM	PF02902	Peptidase_C48	coord: 168..312 e-value: 1.4E-20 score: 74.1
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PROSITE	PS50600	ULP_PROTEASE	coord: 43..288 score: 16.172586
None	No IPR available	GENE3D	3.40.395.10	Adenoviral Proteinase; Chain A	coord: 44..317 e-value: 4.9E-28 score: 100.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 7..22
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR12606	SENTRIN/SUMO-SPECIFIC PROTEASE	coord: 94..315
None	No IPR available	PANTHER	PTHR12606:SF109	BNACNNG52470D PROTEIN	coord: 94..315
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 90..315

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0027708.1	Lag0027708.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0008234	cysteine-type peptidase activity