Lag0027567 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0027567
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	cystinosin homolog
Location	chr8: 2104534 .. 2105007 (+)
RNA-Seq Expression	Lag0027567
Synteny	Lag0027567

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCATGGAATTCAGTTCCGATGGAGATCATATACCAGGTCTTAGGATGGGCCTCCATCATATGCTGGGCACTTGCCGCATATCCGCAGGTCATTCTCAATTTCCGCCGAAAAAGGTATGAGCAATCGATTCTTAATTAATCGATCCTTCACTCCAGCGCCGGCGCCGGAATCGTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTCCGGTGGAGAAATTTAAGAGAGGACTGATGTTCATCAGATTTTGATTTGGCGTAGTGTTGTAGGATTGAGCTTCGATTACGTGATTCTGAATTTCACCAAGCAATCGTCGTATTTGATTTACAACGCTAGTCTCTTCTTCAGCGCCACTGTTCAGAGGCAGTACTTCGAGAAATACGGCTACGATCAGGTTTTCTTCTTCTTCTTCTTCTTCTTGAGTTCTTCTAATTTAAGTTTCGGTACTTCTCGATTGTTTGACGATTCTTGA

mRNA sequence

ATGGCTTCATGGAATTCAGTTCCGATGGAGATCATATACCAGGTCTTAGGATGGGCCTCCATCATATGCTGGGCACTTGCCGCATATCCGCAGGTCATTCTCAATTTCCGCCGAAAAAGTGTTGTAGGATTGAGCTTCGATTACGTGATTCTGAATTTCACCAAGCAATCGTCGTATTTGATTTACAACGCTAGTCTCTTCTTCAGCGCCACTGTTCAGAGGCAGTACTTCGAGAAATACGGCTACGATCAGGTTTTCTTCTTCTTCTTCTTCTTCTTGAGTTCTTCTAATTTAAGTTTCGGTACTTCTCGATTGTTTGACGATTCTTGA

Coding sequence (CDS)

ATGGCTTCATGGAATTCAGTTCCGATGGAGATCATATACCAGGTCTTAGGATGGGCCTCCATCATATGCTGGGCACTTGCCGCATATCCGCAGGTCATTCTCAATTTCCGCCGAAAAAGTGTTGTAGGATTGAGCTTCGATTACGTGATTCTGAATTTCACCAAGCAATCGTCGTATTTGATTTACAACGCTAGTCTCTTCTTCAGCGCCACTGTTCAGAGGCAGTACTTCGAGAAATACGGCTACGATCAGGTTTTCTTCTTCTTCTTCTTCTTCTTGAGTTCTTCTAATTTAAGTTTCGGTACTTCTCGATTGTTTGACGATTCTTGA

Protein sequence

MASWNSVPMEIIYQVLGWASIICWALAAYPQVILNFRRKSVVGLSFDYVILNFTKQSSYLIYNASLFFSATVQRQYFEKYGYDQVFFFFFFFLSSSNLSFGTSRLFDDS

Homology

[Close]

BLAST of Lag0027567 vs. NCBI nr
Match: KAG6588928.1 (Cystinosin-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 158.7 bits (400), Expect = 2.9e-35
Identity = 75/85 (88.24%), Postives = 84/85 (98.82%), Query Frame = 0


Query: 1  MASWNSVPMEIIYQVLGWASIICWALAAYPQVILNFRRKSVVGLSFDYVILNFTKQSSYL 60
          MASWNSVPMEIIYQ+LGW++IICWALA+YPQ+ILNF RKSVVGLSFD+V+LNFTKQSSYL
Sbjct: 1  MASWNSVPMEIIYQLLGWSAIICWALASYPQIILNFWRKSVVGLSFDFVMLNFTKQSSYL 60

Query: 61 IYNASLFFSATVQRQYFEKYGYDQV 86
          IYNASLFFSATVQRQYFEKYG+DQ+
Sbjct: 61 IYNASLFFSATVQRQYFEKYGFDQM 85

[Close]

BLAST of Lag0027567 vs. NCBI nr
Match: XP_022928176.1 (cystinosin homolog [Cucurbita moschata] >XP_022928177.1 cystinosin homolog [Cucurbita moschata])

HSP 1 Score: 157.9 bits (398), Expect = 4.9e-35
Identity = 74/85 (87.06%), Postives = 84/85 (98.82%), Query Frame = 0


Query: 1   MASWNSVPMEIIYQVLGWASIICWALAAYPQVILNFRRKSVVGLSFDYVILNFTKQSSYL 60
           MASWNSVPMEI+YQ+LGW++IICWALA+YPQ+ILNF RKSVVGLSFD+V+LNFTKQSSYL
Sbjct: 33  MASWNSVPMEILYQLLGWSAIICWALASYPQIILNFWRKSVVGLSFDFVMLNFTKQSSYL 92

Query: 61  IYNASLFFSATVQRQYFEKYGYDQV 86
           IYNASLFFSATVQRQYFEKYG+DQ+
Sbjct: 93  IYNASLFFSATVQRQYFEKYGFDQM 117

[Close]

BLAST of Lag0027567 vs. NCBI nr
Match: KAG7022696.1 (Cystinosin-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 157.9 bits (398), Expect = 4.9e-35
Identity = 74/85 (87.06%), Postives = 84/85 (98.82%), Query Frame = 0


Query: 1  MASWNSVPMEIIYQVLGWASIICWALAAYPQVILNFRRKSVVGLSFDYVILNFTKQSSYL 60
          MASWNSVPMEIIYQ+LGW+++ICWALA+YPQ+ILNF RKSVVGLSFD+V+LNFTKQSSYL
Sbjct: 1  MASWNSVPMEIIYQLLGWSALICWALASYPQIILNFWRKSVVGLSFDFVMLNFTKQSSYL 60

Query: 61 IYNASLFFSATVQRQYFEKYGYDQV 86
          IYNASLFFSATVQRQYFEKYG+DQ+
Sbjct: 61 IYNASLFFSATVQRQYFEKYGFDQM 85

[Close]

BLAST of Lag0027567 vs. NCBI nr
Match: XP_023530874.1 (cystinosin homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 157.5 bits (397), Expect = 6.4e-35
Identity = 73/85 (85.88%), Postives = 84/85 (98.82%), Query Frame = 0


Query: 1  MASWNSVPMEIIYQVLGWASIICWALAAYPQVILNFRRKSVVGLSFDYVILNFTKQSSYL 60
          MASWNSVPMEI+YQ+LGW++IICWALA+YPQ++LNF RKSVVGLSFD+V+LNFTKQSSYL
Sbjct: 1  MASWNSVPMEILYQLLGWSAIICWALASYPQIVLNFWRKSVVGLSFDFVMLNFTKQSSYL 60

Query: 61 IYNASLFFSATVQRQYFEKYGYDQV 86
          IYNASLFFSATVQRQYFEKYG+DQ+
Sbjct: 61 IYNASLFFSATVQRQYFEKYGFDQM 85

[Close]

BLAST of Lag0027567 vs. NCBI nr
Match: XP_038880439.1 (cystinosin homolog [Benincasa hispida])

HSP 1 Score: 156.0 bits (393), Expect = 1.9e-34
Identity = 71/85 (83.53%), Postives = 81/85 (95.29%), Query Frame = 0


Query: 1  MASWNSVPMEIIYQVLGWASIICWALAAYPQVILNFRRKSVVGLSFDYVILNFTKQSSYL 60
          M+SWNS+PMEIIY+VLGW SIICWA A YPQ+I+NFRRKSVVGLSFDYV+LNFTKQSSYL
Sbjct: 1  MSSWNSIPMEIIYEVLGWISIICWAFAGYPQLIMNFRRKSVVGLSFDYVVLNFTKQSSYL 60

Query: 61 IYNASLFFSATVQRQYFEKYGYDQV 86
          IYNA+LFFS TVQ+QYF+KYGYDQ+
Sbjct: 61 IYNATLFFSTTVQKQYFDKYGYDQM 85

[Close]

BLAST of Lag0027567 vs. ExPASy Swiss-Prot
Match: P57758 (Cystinosin homolog OS=Arabidopsis thaliana OX=3702 GN=At5g40670 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 1.6e-25
Identity = 49/81 (60.49%), Postives = 68/81 (83.95%), Query Frame = 0


Query: 1  MASWNSVPMEIIYQVLGWASIICWALAAYPQVILNFRRKSVVGLSFDYVILNFTKQSSYL 60
          MASWNS+P+EI Y+++GW +   W+++ YPQ+ILNFRR+SVVGL+FD+V+LN TK SSY+
Sbjct: 1  MASWNSIPLEISYEIVGWIAFASWSISFYPQLILNFRRRSVVGLNFDFVMLNLTKHSSYM 60

Query: 61 IYNASLFFSATVQRQYFEKYG 82
          IYN  L+FS  +Q+QYF+ YG
Sbjct: 61 IYNVCLYFSPVIQKQYFDTYG 81

[Close]

BLAST of Lag0027567 vs. ExPASy Swiss-Prot
Match: Q54WT7 (Cystinosin homolog OS=Dictyostelium discoideum OX=44689 GN=ctns PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.0e-15
Identity = 34/73 (46.58%), Postives = 54/73 (73.97%), Query Frame = 0


Query: 9  MEIIYQVLGWASIICWALAAYPQVILNFRRKSVVGLSFDYVILNFTKQSSYLIYNASLFF 68
          + II  ++GW    CW+L+ YPQVILNFR+K+V+GLSFD+++ N T  + Y ++N+ L+F
Sbjct: 4  LSIISIIIGWIYFACWSLSFYPQVILNFRKKNVIGLSFDFLLFNITGYACYSVFNSVLYF 63

Query: 69 SATVQRQYFEKYG 82
             V+ +Y++KYG
Sbjct: 64 DKLVKNEYYDKYG 76

[Close]

BLAST of Lag0027567 vs. ExPASy Swiss-Prot
Match: A7MB63 (Cystinosin OS=Bos taurus OX=9913 GN=CTNS PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 7.2e-13
Identity = 34/72 (47.22%), Postives = 51/72 (70.83%), Query Frame = 0


Query: 9   MEIIYQVLGWASIICWALAAYPQVILNFRRKSVVGLSFDYVILNFTKQSSYLIYNASLFF 68
           + II QV+GW   + W+++ YPQVI N+RRKSVVGLSFD+V+LN     +Y ++N  LF+
Sbjct: 123 VSIINQVIGWIYFVAWSVSFYPQVITNWRRKSVVGLSFDFVVLNLMGFVAYSVFNIGLFW 182

Query: 69  SATVQRQYFEKY 81
             +++ Q+  KY
Sbjct: 183 VPSIKEQFLLKY 194

[Close]

BLAST of Lag0027567 vs. ExPASy Swiss-Prot
Match: O60931 (Cystinosin OS=Homo sapiens OX=9606 GN=CTNS PE=1 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 1.2e-12
Identity = 34/75 (45.33%), Postives = 51/75 (68.00%), Query Frame = 0


Query: 6   SVPMEIIYQVLGWASIICWALAAYPQVILNFRRKSVVGLSFDYVILNFTKQSSYLIYNAS 65
           S  + II QV+GW   + W+++ YPQVI+N+RRKSV+GLSFD+V LN T   +Y ++N  
Sbjct: 120 SSAISIINQVIGWIYFVAWSISFYPQVIMNWRRKSVIGLSFDFVALNLTGFVAYSVFNIG 179

Query: 66  LFFSATVQRQYFEKY 81
           L +   ++ Q+  KY
Sbjct: 180 LLWVPYIKEQFLLKY 194

[Close]

BLAST of Lag0027567 vs. ExPASy Swiss-Prot
Match: P57757 (Cystinosin OS=Mus musculus OX=10090 GN=Ctns PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 2.3e-11
Identity = 32/72 (44.44%), Postives = 49/72 (68.06%), Query Frame = 0


Query: 9   MEIIYQVLGWASIICWALAAYPQVILNFRRKSVVGLSFDYVILNFTKQSSYLIYNASLFF 68
           + II QV+GW   + W+++ YPQVI N+RRKSV+GLSFD++ LN T   +Y ++N  L +
Sbjct: 123 VSIINQVIGWIYFMAWSVSFYPQVIQNWRRKSVIGLSFDFLALNLTGFVAYSVFNIGLLW 182

Query: 69  SATVQRQYFEKY 81
              +Q ++  KY
Sbjct: 183 VPYIQEEFLLKY 194

The following BLAST results are available for this feature:

BLAST of Lag0027567 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6588928.1	2.9e-35	88.24	Cystinosin-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022928176.1	4.9e-35	87.06	cystinosin homolog [Cucurbita moschata] >XP_022928177.1 cystinosin homolog [Cucu...	[more]
KAG7022696.1	4.9e-35	87.06	Cystinosin-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023530874.1	6.4e-35	85.88	cystinosin homolog [Cucurbita pepo subsp. pepo]	[more]
XP_038880439.1	1.9e-34	83.53	cystinosin homolog [Benincasa hispida]	[more]

BLAST of Lag0027567 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P57758	1.6e-25	60.49	Cystinosin homolog OS=Arabidopsis thaliana OX=3702 GN=At5g40670 PE=2 SV=1	[more]
Q54WT7	2.0e-15	46.58	Cystinosin homolog OS=Dictyostelium discoideum OX=44689 GN=ctns PE=3 SV=1	[more]
A7MB63	7.2e-13	47.22	Cystinosin OS=Bos taurus OX=9913 GN=CTNS PE=2 SV=1	[more]
O60931	1.2e-12	45.33	Cystinosin OS=Homo sapiens OX=9606 GN=CTNS PE=1 SV=2	[more]
P57757	2.3e-11	44.44	Cystinosin OS=Mus musculus OX=10090 GN=Ctns PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006603	PQ-loop repeat	SMART	SM00679	ctns	coord: 26..57 e-value: 5.0E-5 score: 32.7
IPR006603	PQ-loop repeat	PFAM	PF04193	PQ-loop	coord: 13..68 e-value: 2.9E-18 score: 65.4
None	No IPR available	GENE3D	1.20.1280.290		coord: 12..104 e-value: 8.1E-9 score: 37.5
None	No IPR available	PANTHER	PTHR13131:SF12	LYSOSOMAL CYSTINE TRANSPORTER FAMILY PROTEIN	coord: 1..84
IPR005282	Lysosomal cystine transporter	PANTHER	PTHR13131	CYSTINOSIN	coord: 1..84

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0027567.1	Lag0027567.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015811	L-cystine transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005774	vacuolar membrane
molecular_function	GO:0015184	L-cystine transmembrane transporter activity