Lag0027469 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0027469
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptioncation/H(+) antiporter 15-like
Locationchr8: 1154807 .. 1157369 (-)
RNA-Seq ExpressionLag0027469
SyntenyLag0027469
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCGATTGTAATGGAACCCGGCGACATCGCCGCCTTCAAGAACTCCACCTCCCTCTGTACATTCGCCGGTCGAATCCACAGCGGCGGCGTTTTCGCCGGAGCCAACCCATTGGAGTTCTCAGTTCCTCTTCTCTTCTTGCAGCTCGGAATCTGCGCTGGAACCATCATCTTGTTCTCTCGCCTTCTCAAGCCCTTCGGCCAACCCCTCATCGTCTCGCAGATTCTGGTAATCTCTCCTCTTTTCTTCAACCCCTTTATACCAAATTTCATTCTTCAAGTTTCTTATTCGTTGTATTTGTGATTTCATCAGGGCGCTTTGGTTCTTGGCTCTTCGGGTCTGTGCCACTTGGAGAAATTTAGAGAGGCGATTTTCCCTTTGAGGGGATTCATCATACTGGATGTGGTCTCTGCGCTTGGCCATGTCTTTTACTTCTTTCTGATTGGAGTGCAAACGGATTTCTCCATTGTGAAGAGGATTGACAAGAAAGCATTTGGTATTGGGTTTTGCTCTGTGATTTCGCCTTCCATTCTCACTTTCATTTTCCCTTTCACCTTGATTAATATTGTTGACTCTCAAACTCTTCGATCCCTTGCAATTATTGGTAGAGCAGAGGTTTTCATCAACTTCACGATGGTTGCTTCCCTTCTTTCTGAGCTTCATTTGATAAACTCTAAGTTTGGGAGAATTGCCTTGTCGTCTTCCATGGTTTCCAATTTCGTTAGCTTTTTTCTCCTGAAACTAGGAGCTCTCTTGACAACAGAGGCTAGATATAAGGTCTTGTCGACACTTTATTCTTTTGTAGTGGTTGTAGTTGTCATCATTTTCGTTATTCGACCTGCCATTTTGTGGATGATAAAGCAGCATCCAGTGGGACAGCCATTGAAGGAGGGCTTTGTGATTACATTGCTTTTAGTGGTGCTAGCAGCTGCTTTTTGTAGCAAGGCCTTTGGTTTGCATATCTATTTTGGTCCTCTTTTACTTGGGGTTACAATACCTTCAGGGCCTCCCCTTGGATCAGCATTGGTGGAGAGGCTTGATTTCATCACCTCCTGGCTTTTCATGCCCATCTTCTTTGTCAAAATAGGCTTGGTTGTCGATATCTATGCCATCGAGCTCATAAATTTCTTATGCACCTCGTTCATTATCTTTCTCAGTGCATTGGGGAAGTTTTTGGGTGCCTTGTTGGCTTCAATCTACTTCAAACTACCTATGAGGGACGCCGTATCGCTCGGCCTCGTCTTGAATAGCCAAGGAGCTTTCGAGCTTGGTATGTATAAAATGATGAAGAAGGACAAGGTAGGAACAAGAAAATTTGTTACTGATCATCATTATCTAATTACATCTAGTCATTTCTCACAAACTCACTGCCATTTTGTTTCCTAAATTCTTGCAGGCGATAAATGATGAAACATTTGTAGTTGCCTGCATATTCTTGTTGGTTCTGGCTGCAGTTATAGCTCCCATACTAAGATATCTCTTTGATCCTTCTAGGAGGTATGTAGTTTACAAGAGAAGAACTGTGATGCACTCTAGACCAGAGTTTGATCTGTGTGTATTAGTCTGCATTCATGACCAAGAAGATGTTCCAAGTGCCATTAACCTACTTGACGCCTTGAATCCGACGAGACGAAGCCATCTCTATGTGTACATGCTTCATCTTGTCGAGCTTCTCGGTCGTGCTACCCCGCAACTCATTCACCACAAGCTTACGAAAGTAAGGACTTCAAGGCATTCCCCTTCTGAGCCTATTGTTAATGCCTTCAAATACTTTGGACAGCGCAACCGTGAAATTGTTAAAATTTATCCCTTCACTGCAATATCACCTCCTGCATCTATGCACGATGATGTTTGTTCCCTTGCACATGAAAAGAAGACTTCCTTGATTCTTGTTCCTTTCCACAAAAGATTTCATTCCAACGGCGTGATGTCGTTGTCCAAAAACAAAATGAAAATGGTTAACCTTCATATCCTCGACAAGGCACCCTGCTCGGTCGCCCTTGTTGTCGAGCGTGGAATTTTGAACGTCTCGAGGTCTATTGCTACCAATTTGCATCCCTTTCAGGTTGTTGTAGTCTTCATAGGTGGACCAGACGACCGTGAGGCAATGTTCATCGGAGCAAGGATGGTTGAACATCCCAATATCAACTTGACAATGATTCGACTGTTGGAGGACATGGATATCCCAAGCGATAACGTTAACGAGAGGATGCTCGATAACGAGGCTGTGTTTGAGTTTCAACAAATCATGGCTAACAACTATAGGGTGAGGTACATAGAAGAGGTGGTTAAAGACAGCACTGGAACGGTCTCGATACTTCGTTCGATGGGGAACAATTTCGATCTCGTAATGGTCGGAAGACGACATAGTCCGTGTTCCGTGATGGTTCAAGGCTTGGTATTTTGGGATGAGCAAACAGAACTTGGGATAATTGGGGAGGCATTGGCCTCTTCAGATTTCATGGGCAATACCATGATCTTGGTTGTGCAACAACACACAAATGTGGTAGATGAAGACCAATGGAATCATCAAGAAACTATCAATATACTTAGAAGATGA

mRNA sequence

ATGGGTTCGATTGTAATGGAACCCGGCGACATCGCCGCCTTCAAGAACTCCACCTCCCTCTGTACATTCGCCGGTCGAATCCACAGCGGCGGCGTTTTCGCCGGAGCCAACCCATTGGAGTTCTCAGTTCCTCTTCTCTTCTTGCAGCTCGGAATCTGCGCTGGAACCATCATCTTGTTCTCTCGCCTTCTCAAGCCCTTCGGCCAACCCCTCATCGTCTCGCAGATTCTGGGCGCTTTGGTTCTTGGCTCTTCGGGTCTGTGCCACTTGGAGAAATTTAGAGAGGCGATTTTCCCTTTGAGGGGATTCATCATACTGGATGTGGTCTCTGCGCTTGGCCATGTCTTTTACTTCTTTCTGATTGGAGTGCAAACGGATTTCTCCATTGTGAAGAGGATTGACAAGAAAGCATTTGGTATTGGGTTTTGCTCTGTGATTTCGCCTTCCATTCTCACTTTCATTTTCCCTTTCACCTTGATTAATATTGTTGACTCTCAAACTCTTCGATCCCTTGCAATTATTGGTAGAGCAGAGGTTTTCATCAACTTCACGATGGTTGCTTCCCTTCTTTCTGAGCTTCATTTGATAAACTCTAAGTTTGGGAGAATTGCCTTGTCGTCTTCCATGGTTTCCAATTTCGTTAGCTTTTTTCTCCTGAAACTAGGAGCTCTCTTGACAACAGAGGCTAGATATAAGGTCTTGTCGACACTTTATTCTTTTGTAGTGGTTGTAGTTGTCATCATTTTCGTTATTCGACCTGCCATTTTGTGGATGATAAAGCAGCATCCAGTGGGACAGCCATTGAAGGAGGGCTTTGTGATTACATTGCTTTTAGTGGTGCTAGCAGCTGCTTTTTGTAGCAAGGCCTTTGGTTTGCATATCTATTTTGGTCCTCTTTTACTTGGGGTTACAATACCTTCAGGGCCTCCCCTTGGATCAGCATTGGTGGAGAGGCTTGATTTCATCACCTCCTGGCTTTTCATGCCCATCTTCTTTGTCAAAATAGGCTTGGTTGTCGATATCTATGCCATCGAGCTCATAAATTTCTTATGCACCTCGTTCATTATCTTTCTCAGTGCATTGGGGAAGTTTTTGGGTGCCTTGTTGGCTTCAATCTACTTCAAACTACCTATGAGGGACGCCGTATCGCTCGGCCTCGTCTTGAATAGCCAAGGAGCTTTCGAGCTTGGTATGTATAAAATGATGAAGAAGGACAAGGCGATAAATGATGAAACATTTGTAGTTGCCTGCATATTCTTGTTGGTTCTGGCTGCAGTTATAGCTCCCATACTAAGATATCTCTTTGATCCTTCTAGGAGGTATGTAGTTTACAAGAGAAGAACTGTGATGCACTCTAGACCAGAGTTTGATCTGTGTGTATTAGTCTGCATTCATGACCAAGAAGATGTTCCAAGTGCCATTAACCTACTTGACGCCTTGAATCCGACGAGACGAAGCCATCTCTATGTGTACATGCTTCATCTTGTCGAGCTTCTCGGTCGTGCTACCCCGCAACTCATTCACCACAAGCTTACGAAAGTAAGGACTTCAAGGCATTCCCCTTCTGAGCCTATTGTTAATGCCTTCAAATACTTTGGACAGCGCAACCGTGAAATTGTTAAAATTTATCCCTTCACTGCAATATCACCTCCTGCATCTATGCACGATGATGTTTGTTCCCTTGCACATGAAAAGAAGACTTCCTTGATTCTTGTTCCTTTCCACAAAAGATTTCATTCCAACGGCGTGATGTCGTTGTCCAAAAACAAAATGAAAATGGTTAACCTTCATATCCTCGACAAGGCACCCTGCTCGGTCGCCCTTGTTGTCGAGCGTGGAATTTTGAACGTCTCGAGGTCTATTGCTACCAATTTGCATCCCTTTCAGGTTGTTGTAGTCTTCATAGGTGGACCAGACGACCGTGAGGCAATGTTCATCGGAGCAAGGATGGTTGAACATCCCAATATCAACTTGACAATGATTCGACTGTTGGAGGACATGGATATCCCAAGCGATAACGTTAACGAGAGGATGCTCGATAACGAGGCTGTGTTTGAGTTTCAACAAATCATGGCTAACAACTATAGGGTGAGGTACATAGAAGAGGTGGTTAAAGACAGCACTGGAACGGTCTCGATACTTCGTTCGATGGGGAACAATTTCGATCTCGTAATGGTCGGAAGACGACATAGTCCGTGTTCCGTGATGGTTCAAGGCTTGGTATTTTGGGATGAGCAAACAGAACTTGGGATAATTGGGGAGGCATTGGCCTCTTCAGATTTCATGGGCAATACCATGATCTTGGTTGTGCAACAACACACAAATGTGGTAGATGAAGACCAATGGAATCATCAAGAAACTATCAATATACTTAGAAGATGA

Coding sequence (CDS)

ATGGGTTCGATTGTAATGGAACCCGGCGACATCGCCGCCTTCAAGAACTCCACCTCCCTCTGTACATTCGCCGGTCGAATCCACAGCGGCGGCGTTTTCGCCGGAGCCAACCCATTGGAGTTCTCAGTTCCTCTTCTCTTCTTGCAGCTCGGAATCTGCGCTGGAACCATCATCTTGTTCTCTCGCCTTCTCAAGCCCTTCGGCCAACCCCTCATCGTCTCGCAGATTCTGGGCGCTTTGGTTCTTGGCTCTTCGGGTCTGTGCCACTTGGAGAAATTTAGAGAGGCGATTTTCCCTTTGAGGGGATTCATCATACTGGATGTGGTCTCTGCGCTTGGCCATGTCTTTTACTTCTTTCTGATTGGAGTGCAAACGGATTTCTCCATTGTGAAGAGGATTGACAAGAAAGCATTTGGTATTGGGTTTTGCTCTGTGATTTCGCCTTCCATTCTCACTTTCATTTTCCCTTTCACCTTGATTAATATTGTTGACTCTCAAACTCTTCGATCCCTTGCAATTATTGGTAGAGCAGAGGTTTTCATCAACTTCACGATGGTTGCTTCCCTTCTTTCTGAGCTTCATTTGATAAACTCTAAGTTTGGGAGAATTGCCTTGTCGTCTTCCATGGTTTCCAATTTCGTTAGCTTTTTTCTCCTGAAACTAGGAGCTCTCTTGACAACAGAGGCTAGATATAAGGTCTTGTCGACACTTTATTCTTTTGTAGTGGTTGTAGTTGTCATCATTTTCGTTATTCGACCTGCCATTTTGTGGATGATAAAGCAGCATCCAGTGGGACAGCCATTGAAGGAGGGCTTTGTGATTACATTGCTTTTAGTGGTGCTAGCAGCTGCTTTTTGTAGCAAGGCCTTTGGTTTGCATATCTATTTTGGTCCTCTTTTACTTGGGGTTACAATACCTTCAGGGCCTCCCCTTGGATCAGCATTGGTGGAGAGGCTTGATTTCATCACCTCCTGGCTTTTCATGCCCATCTTCTTTGTCAAAATAGGCTTGGTTGTCGATATCTATGCCATCGAGCTCATAAATTTCTTATGCACCTCGTTCATTATCTTTCTCAGTGCATTGGGGAAGTTTTTGGGTGCCTTGTTGGCTTCAATCTACTTCAAACTACCTATGAGGGACGCCGTATCGCTCGGCCTCGTCTTGAATAGCCAAGGAGCTTTCGAGCTTGGTATGTATAAAATGATGAAGAAGGACAAGGCGATAAATGATGAAACATTTGTAGTTGCCTGCATATTCTTGTTGGTTCTGGCTGCAGTTATAGCTCCCATACTAAGATATCTCTTTGATCCTTCTAGGAGGTATGTAGTTTACAAGAGAAGAACTGTGATGCACTCTAGACCAGAGTTTGATCTGTGTGTATTAGTCTGCATTCATGACCAAGAAGATGTTCCAAGTGCCATTAACCTACTTGACGCCTTGAATCCGACGAGACGAAGCCATCTCTATGTGTACATGCTTCATCTTGTCGAGCTTCTCGGTCGTGCTACCCCGCAACTCATTCACCACAAGCTTACGAAAGTAAGGACTTCAAGGCATTCCCCTTCTGAGCCTATTGTTAATGCCTTCAAATACTTTGGACAGCGCAACCGTGAAATTGTTAAAATTTATCCCTTCACTGCAATATCACCTCCTGCATCTATGCACGATGATGTTTGTTCCCTTGCACATGAAAAGAAGACTTCCTTGATTCTTGTTCCTTTCCACAAAAGATTTCATTCCAACGGCGTGATGTCGTTGTCCAAAAACAAAATGAAAATGGTTAACCTTCATATCCTCGACAAGGCACCCTGCTCGGTCGCCCTTGTTGTCGAGCGTGGAATTTTGAACGTCTCGAGGTCTATTGCTACCAATTTGCATCCCTTTCAGGTTGTTGTAGTCTTCATAGGTGGACCAGACGACCGTGAGGCAATGTTCATCGGAGCAAGGATGGTTGAACATCCCAATATCAACTTGACAATGATTCGACTGTTGGAGGACATGGATATCCCAAGCGATAACGTTAACGAGAGGATGCTCGATAACGAGGCTGTGTTTGAGTTTCAACAAATCATGGCTAACAACTATAGGGTGAGGTACATAGAAGAGGTGGTTAAAGACAGCACTGGAACGGTCTCGATACTTCGTTCGATGGGGAACAATTTCGATCTCGTAATGGTCGGAAGACGACATAGTCCGTGTTCCGTGATGGTTCAAGGCTTGGTATTTTGGGATGAGCAAACAGAACTTGGGATAATTGGGGAGGCATTGGCCTCTTCAGATTTCATGGGCAATACCATGATCTTGGTTGTGCAACAACACACAAATGTGGTAGATGAAGACCAATGGAATCATCAAGAAACTATCAATATACTTAGAAGATGA

Protein sequence

MGSIVMEPGDIAAFKNSTSLCTFAGRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLCHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDFSIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDSQTLRSLAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLTTEARYKVLSTLYSFVVVVVVIIFVIRPAILWMIKQHPVGQPLKEGFVITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELINFLCTSFIIFLSALGKFLGALLASIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQEDVPSAINLLDALNPTRRSHLYVYMLHLVELLGRATPQLIHHKLTKVRTSRHSPSEPIVNAFKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKNKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINLTMIRLLEDMDIPSDNVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDSTGTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMILVVQQHTNVVDEDQWNHQETINILRR
Homology
BLAST of Lag0027469 vs. NCBI nr
Match: XP_022983542.1 (cation/H(+) antiporter 15-like [Cucurbita maxima])

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 574/800 (71.75%), Postives = 672/800 (84.00%), Query Frame = 0

Query: 1   MGSIVMEPGDIAAF----------KNSTSLCTFAGRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSI+MEP D+AA+          KN T +CTFA RIHS GVF+G NPLEFSVPLLFLQL
Sbjct: 1   MGSIIMEPDDVAAYISGCDSHNTLKNITRICTFADRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLCHLEKFREAIFPLRGFIILDVVS 120
           G+ AGTIILFS LLKP GQPLIVSQILG +VLGSSGL HL +FRE IFPLRGF++LDV+S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGHVFYFFLIGVQTDFSIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDSQTLRS 180
           ALGHVF+FFLIGVQTD S VK+IDKKAF IG CSVI   IL+ I+  TL+NIVD QT++ 
Sbjct: 121 ALGHVFFFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLT--TE 240
           L  IG  E FINF MVASL+SELHLINS+FGR ALS++M SNF S  L  LGALLT   E
Sbjct: 181 LFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLTPQDE 240

Query: 241 ARYKVLSTLYSFVVVVVVIIFVIRPAILWMIKQHPVGQPLKEGFVITLLLVVLAAAFCSK 300
            +Y+ +STLY+ +++V VI F IRP I+WMIK++P+GQPLKE FV+TLLL+VL  AFC +
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELIN 360
           A GLHIY GPL LGVTIPSGPP+GSALV+RLDFITSW+FMPIFFVKIGL V+IYAI+LIN
Sbjct: 301 ALGLHIYTGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLAVNIYAIKLIN 360

Query: 361 FLCTSFIIFLSALGKFLGALLASIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAI 420
           FLC SFI+F++ALGKFLGALL S YFKL MRDAVSLGL+LN QGA ELG++K+M+K+K I
Sbjct: 361 FLCMSFIVFVNALGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQE 480
           NDE  VV C++++V+ A+I+PI+R+L DPSRR++V+KRRTVMHSRPEFDLCVLVCIHDQE
Sbjct: 421 NDEALVVMCVWVMVVVAIISPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCVLVCIHDQE 480

Query: 481 DVPSAINLLDALNPTRRSHLYVYMLHLVELLGRATPQLIHHKLTKVRTSRHSPSEPIVNA 540
           DVPSAINLLDALNPTRRSHL VY+LHLVELLGRA  +LI+HK  +VRTSR   S PIVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSK 600
           FKYFG+  REI+ IYPFTAISP  +MHD VCSLA +KKTSLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKREILAIYPFTAISPSTTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 NKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGA 660
            K KMVN HIL+ APCSVALVVERGIL   +SIATN HPFQ+V+VFIGGPDDREAMFIGA
Sbjct: 601 YKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIVMVFIGGPDDREAMFIGA 660

Query: 661 RMVEHPNINLTMIRLLEDMDIPSDNVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDST 720
           RMV HPNINLT IRL E  ++PS +V ER LDNE+V EF+Q +A+N RV+YIEEVVKD  
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEVVKDGI 720

Query: 721 GTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMIL 780
           GT+SILRS+G +FDLV+VGR+H+PC  +VQGLV W+EQTELG IGE LASSDF+GN  IL
Sbjct: 721 GTISILRSLGGDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTNVVDEDQWNHQETI 789
           VVQQH  V++ED+  +QE I
Sbjct: 781 VVQQHRRVINEDEELNQEDI 800

BLAST of Lag0027469 vs. NCBI nr
Match: XP_023528659.1 (cation/H(+) antiporter 15-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 570/800 (71.25%), Postives = 668/800 (83.50%), Query Frame = 0

Query: 1   MGSIVMEPGDIAAF----------KNSTSLCTFAGRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSI+MEP D+AA+          KN T +CTFA RIHS GVF+G NPLEFSVPLLFLQL
Sbjct: 1   MGSIIMEPDDVAAYISGGHGHNTLKNLTKICTFANRIHSPGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLCHLEKFREAIFPLRGFIILDVVS 120
           G+ AGTII FS LLKP GQPLIVSQILG +VLGSSGL HL +FRE IFPLRGF++LDV+S
Sbjct: 61  GLSAGTIIFFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGHVFYFFLIGVQTDFSIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDSQTLRS 180
           ALGHVFYFFLIGVQTD S VK+IDKKAF IG CSVI   IL+ I+  TL+NIVD QT++ 
Sbjct: 121 ALGHVFYFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTIKY 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLT--TE 240
           L  IG  E FINF MVASL+SELHLINS+FGR ALS++M SNF S  L  LGALL    E
Sbjct: 181 LFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLAPQNE 240

Query: 241 ARYKVLSTLYSFVVVVVVIIFVIRPAILWMIKQHPVGQPLKEGFVITLLLVVLAAAFCSK 300
            +Y+ +STLY+ +++V VI F IRP I+WMIK++P+G+PLKE FV+TLLL+VL  AFC +
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPIIVWMIKKNPIGKPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELIN 360
           A GLHIY GPL LGVTIPSGPP+GSALV+RLDFITSW+FMPIFFVKIGLVV+IYA +LIN
Sbjct: 301 ALGLHIYIGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLVVNIYATKLIN 360

Query: 361 FLCTSFIIFLSALGKFLGALLASIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAI 420
           FLC SFI+F+ A+GKFLGALL S YFKL MRDAVSLGL+LN QGA ELG++K+M+K+K I
Sbjct: 361 FLCMSFIVFVGAMGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQE 480
           NDE  VV C++++V+ A+I PI+R+L DPSRR++V+KRRTVMHSRPEFDLCVLVCIHDQE
Sbjct: 421 NDEALVVTCVWVMVVVAIITPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCVLVCIHDQE 480

Query: 481 DVPSAINLLDALNPTRRSHLYVYMLHLVELLGRATPQLIHHKLTKVRTSRHSPSEPIVNA 540
           DVPSAINLLDALNPTRRSHL VY+LHLVELLGRA  +LIHHK  +VRTSR   S PIVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLAVYILHLVELLGRAHLELIHHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSK 600
           FKYFG+   EI+ IYPFTAISP A+MHD VCSLA +KKTSLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKSEILAIYPFTAISPSATMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 NKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGA 660
            K KMVN HIL+ APCS+ALVVERGIL   +SIATN HPFQ+ +VFIGGPDDREAMFIGA
Sbjct: 601 YKTKMVNHHILENAPCSIALVVERGILKTPKSIATNFHPFQIAMVFIGGPDDREAMFIGA 660

Query: 661 RMVEHPNINLTMIRLLEDMDIPSDNVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDST 720
           RMV HPNINLT IRL E  ++PS +V ER LDN++V EF+Q +A+N RV+YIEE+VKD  
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNKSVDEFRQSIADNNRVKYIEEMVKDGI 720

Query: 721 GTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMIL 780
           GT+SILRSMG++FDLV+VGR+H+PC  +VQGLV W+EQTELG IGE LASSDF+GN  IL
Sbjct: 721 GTISILRSMGSDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTNVVDEDQWNHQETI 789
           VVQQH  VV+ED+  +QE I
Sbjct: 781 VVQQHRRVVNEDEELNQEDI 800

BLAST of Lag0027469 vs. NCBI nr
Match: XP_022934362.1 (cation/H(+) antiporter 15-like [Cucurbita moschata])

HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 571/800 (71.38%), Postives = 670/800 (83.75%), Query Frame = 0

Query: 1   MGSIVMEPGDIAAF----------KNSTSLCTFAGRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSI+MEP D+AA+          KN T +CTFA RIHS GVF+G NPLEFSVPLLFLQL
Sbjct: 1   MGSIIMEPDDVAAYISGGHGQNTLKNLTRICTFANRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLCHLEKFREAIFPLRGFIILDVVS 120
           G+ AGTIILFS LLKP GQPLIVSQILG +VLGSSGL HL +FRE IFPLRGF++LDV+S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGHVFYFFLIGVQTDFSIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDSQTLRS 180
           ALGHVFYFFLIGVQTD S VK+IDKKAF IG CSVI   IL+ I+  TL+NIVD QT++ 
Sbjct: 121 ALGHVFYFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLT--TE 240
           L  IG  E FINF MVASL+SELHLINS+FGR ALS++M SNF S  L  LGALL   +E
Sbjct: 181 LFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLAPQSE 240

Query: 241 ARYKVLSTLYSFVVVVVVIIFVIRPAILWMIKQHPVGQPLKEGFVITLLLVVLAAAFCSK 300
            +Y+ +STLY+ +++V VI F IRP I+WMIK++P+GQPLKE FV+TLLL+VL  AFC +
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELIN 360
           A GLHIY GPL LGVTIPSGPP+GSALV+RLDFITSW+FMPIFFVKIGLVV+IYA +LIN
Sbjct: 301 ASGLHIYIGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLVVNIYATKLIN 360

Query: 361 FLCTSFIIFLSALGKFLGALLASIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAI 420
           FLC SFI+F+SA+GKFLGALL S YFKL MRDAVSLGL+LN QGA ELG++K+M+K+K I
Sbjct: 361 FLCMSFIVFVSAMGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQE 480
           NDE  VV C++++V+ A+I PI+R+L DPSRR++V+KRRTVMHSRPEFDLC LVCIHDQE
Sbjct: 421 NDEALVVTCVWVMVVVAIITPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCALVCIHDQE 480

Query: 481 DVPSAINLLDALNPTRRSHLYVYMLHLVELLGRATPQLIHHKLTKVRTSRHSPSEPIVNA 540
           DVPSAINLLDALNPTRRSHL VY+LHLVELLGRA  +LI+HK  +VRTSR   S PIVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSK 600
           FKYFG+   EI+ IYPFTAISP ++MHD VCSLA +KKTSLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKSEILAIYPFTAISPSSTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 NKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGA 660
            K KMVN HIL+ APCSVALVVERGIL   +SIATN HPFQ+ +VFIGGPDDREAMFIGA
Sbjct: 601 YKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIAMVFIGGPDDREAMFIGA 660

Query: 661 RMVEHPNINLTMIRLLEDMDIPSDNVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDST 720
           RMV HPNINLT IRL E  ++PS +V ER LDNE+V EF+Q +A+N RV+YIEE+VKD  
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEMVKDGI 720

Query: 721 GTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMIL 780
           GT+SILRS+G++FDLV+VGR+H+PC  +VQGLV W+EQTELG IGE LASSDF+GN  IL
Sbjct: 721 GTISILRSLGSDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTNVVDEDQWNHQETI 789
           VVQQH  VV+ED+  +QE I
Sbjct: 781 VVQQHRRVVNEDEELNQEDI 800

BLAST of Lag0027469 vs. NCBI nr
Match: KAG6581108.1 (Cation/H(+) antiporter 15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1104.0 bits (2854), Expect = 0.0e+00
Identity = 568/800 (71.00%), Postives = 670/800 (83.75%), Query Frame = 0

Query: 1   MGSIVMEPGDIAAF----------KNSTSLCTFAGRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSI+MEP D+AA+          KN T +CTFA RIHS GVF+G NPLEFSVPLLFLQL
Sbjct: 1   MGSIIMEPDDVAAYISGGHGQNTLKNLTRICTFANRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLCHLEKFREAIFPLRGFIILDVVS 120
           G+ AGTIILFS LLKP GQPLIVSQILG +VLGSSGL HL +FRE IFPLRGF++LDV+S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGHVFYFFLIGVQTDFSIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDSQTLRS 180
           ALGHVFYFFLIGVQTD S VK+IDKKAF IG CSVI   IL+ I+  TL+NIVD QT++ 
Sbjct: 121 ALGHVFYFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLT--TE 240
           L  IG  E  INF MVASL+SELHLINS+FGR++LS++M SNF S  L  LGALL   +E
Sbjct: 181 LFAIGGTESLINFPMVASLVSELHLINSEFGRLSLSTAMASNFFSICLALLGALLAPQSE 240

Query: 241 ARYKVLSTLYSFVVVVVVIIFVIRPAILWMIKQHPVGQPLKEGFVITLLLVVLAAAFCSK 300
            +Y+ +STLY+ +++V VI F IRP I+WMIK++P+GQPLKE FV+TLLL+VL  AFC +
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELIN 360
           A GLHIY GPL LGVTIPSGPP+GSALV+RLDFITSW+FMPIFFVKIGLVV+IYA +LIN
Sbjct: 301 ALGLHIYIGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLVVNIYATKLIN 360

Query: 361 FLCTSFIIFLSALGKFLGALLASIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAI 420
           FLC SFI+F+SA+GKFLGALL S YFKL MRDAVSLGL+LN QGA ELG++K+M+K+K I
Sbjct: 361 FLCMSFIVFVSAMGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQE 480
           NDE  VV C++++V+ A+I PI+R+L DPSRR++V+KRRTVMHSRPEFDLC LVCIHDQE
Sbjct: 421 NDEALVVTCVWVMVVVAIITPIIRHLLDPSRRFLVHKRRTVMHSRPEFDLCALVCIHDQE 480

Query: 481 DVPSAINLLDALNPTRRSHLYVYMLHLVELLGRATPQLIHHKLTKVRTSRHSPSEPIVNA 540
           DVPSAINLLDALNPTRRSHL V++LHLVELLGRA  +LI+HK  +VRTSR   S PIVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLVVHILHLVELLGRAHVELIYHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSK 600
           FKYFG+   EI+ IYPFTAISP ++MHD VCSLA +KKTSLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKSEILAIYPFTAISPSSTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 NKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGA 660
            K KMVN HIL+ APCSVALVVERGIL   +SIATN HPFQ+ +VFIGGPDDREAMFIGA
Sbjct: 601 YKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIAMVFIGGPDDREAMFIGA 660

Query: 661 RMVEHPNINLTMIRLLEDMDIPSDNVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDST 720
           RMV HPNINLT IRL E  ++PS +V ER LDNE+V EF+Q +A+N RV+YIEE+VKD  
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEMVKDGI 720

Query: 721 GTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMIL 780
           GT+SILRS+G++FDLV+VGR+H+PC  +VQGLV W+EQTELG IGE LASSDF+GN  IL
Sbjct: 721 GTISILRSLGSDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTNVVDEDQWNHQETI 789
           VVQQH  VV+ED+  +QE I
Sbjct: 781 VVQQHRRVVNEDEELNQEDI 800

BLAST of Lag0027469 vs. NCBI nr
Match: KAG7017840.1 (Cation/H(+) antiporter 15 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 568/812 (69.95%), Postives = 670/812 (82.51%), Query Frame = 0

Query: 1   MGSIVMEPGDIAAF----------KNSTSLCTFAGRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSI+MEP D+AA+          KN T +CTFA RIHS GVF+G NPLEFSVPLLFLQL
Sbjct: 1   MGSIIMEPDDVAAYISGGHGQNTLKNLTRICTFANRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLCHLEKFREAIFPLRGFIILDVVS 120
           G+ AGTIILFS LLKP GQPLIVSQILG +VLGSSGL HL +FRE IFPLRGF++LDV+S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGHVFYFFLIGVQTDFSIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDSQTLRS 180
           ALGHVFYFFLIGVQTD S VK+IDKKAF IG CSVI   IL+ I+  TL+NIVD QT++ 
Sbjct: 121 ALGHVFYFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLT--TE 240
           L  IG  E  INF MVASL+SELHLINS+FGR++LS++M SNF S  L  LGALL   +E
Sbjct: 181 LFAIGGTESLINFPMVASLVSELHLINSEFGRLSLSTAMASNFFSICLALLGALLAPQSE 240

Query: 241 ARYKVLSTLYSFVVVVVVIIFVIRPAILWMIKQHPVGQPLKEGFVITLLLVVLAAAFCSK 300
            +Y+ +STLY+ +++V VI F IRP I+WMIK++P+GQPLKE FV+TLLL+VL  AFC +
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELIN 360
           A GLHIY GPL LGVTIPSGPP+GSALV+RLDFITSW+FMPIFFVKIGLVV+IYA +LIN
Sbjct: 301 ALGLHIYIGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLVVNIYATKLIN 360

Query: 361 FLCTSFIIFLSALGKFLGALLASIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDK-- 420
           FLC SFI+F+SA+GKFLGALL S YFKL MRDAVSLGL+LN QGA ELG++K+M+K+K  
Sbjct: 361 FLCMSFIVFVSAMGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKEL 420

Query: 421 ----------AINDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEF 480
                      INDE  VV C++++V+ A+I PI+R+L DPSRR++V+KRRTVMHSRPEF
Sbjct: 421 IASLFLESTQLINDEALVVTCVWVMVVVAIITPIIRHLLDPSRRFLVHKRRTVMHSRPEF 480

Query: 481 DLCVLVCIHDQEDVPSAINLLDALNPTRRSHLYVYMLHLVELLGRATPQLIHHKLTKVRT 540
           DLC LVCIHDQEDVPSAINLLDALNPTRRSHL VY+LHLVELLGRA  +LI+HK  +VRT
Sbjct: 481 DLCALVCIHDQEDVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRT 540

Query: 541 SRHSPSEPIVNAFKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHK 600
           SR   S PIVNAFKYFG+   EI+ IYPFTAISP ++MHD VCSLA +KKTSLILVPFH+
Sbjct: 541 SRSCSSIPIVNAFKYFGESKSEILAIYPFTAISPSSTMHDVVCSLALDKKTSLILVPFHQ 600

Query: 601 RFHSNGVMSLSKNKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIG 660
           RFHSNGV+SLS+ K KMVN HIL+ APCSVALVVERGIL   +SIA+N HPFQ+ +VFIG
Sbjct: 601 RFHSNGVLSLSQYKTKMVNHHILENAPCSVALVVERGILKTPKSIASNFHPFQIAMVFIG 660

Query: 661 GPDDREAMFIGARMVEHPNINLTMIRLLEDMDIPSDNVNERMLDNEAVFEFQQIMANNYR 720
           GPDDREAMFIGARMV HPNINLT IRL E  ++PS +V ER LDNE+V EF+Q +A+N R
Sbjct: 661 GPDDREAMFIGARMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNR 720

Query: 721 VRYIEEVVKDSTGTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEAL 780
           V+YIEE+VKD  GT+SILRS+G++FDLV+VGR+H+PC  +VQGLV W+EQTELG IGE L
Sbjct: 721 VKYIEEMVKDGIGTISILRSLGSDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVL 780

Query: 781 ASSDFMGNTMILVVQQHTNVVDEDQWNHQETI 789
           ASSDF+GN  ILVVQQH  VV+ED+  +QE I
Sbjct: 781 ASSDFIGNATILVVQQHRRVVNEDEELNQEDI 812

BLAST of Lag0027469 vs. ExPASy Swiss-Prot
Match: Q9SIT5 (Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 4.7e-138
Identity = 291/798 (36.47%), Postives = 471/798 (59.02%), Query Frame = 0

Query: 16  NSTSLCTFAGRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLIVSQ 75
           +++ +C     I + GV+ G NPL+FS+PL  LQL +       F  +LKPF QP ++S+
Sbjct: 10  DASIICYAPSMITTNGVWQGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQPRVISE 69

Query: 76  ILGALVLGSSGLCHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDFSIVKRIDK 135
           ILG +VLG S L    KF   IFP R  ++L+ ++ +G +++ FL+GV+ D  +V++  K
Sbjct: 70  ILGGIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMVVRKTGK 129

Query: 136 KAFGIGFCSVISPSILTFIFPFTLINIVDSQTLRS-LAIIGRAEVFINFTMVASLLSELH 195
           +A  I    ++ P ++   F F++    D     + +  +G A     F ++A +L+EL 
Sbjct: 130 RALTIAIGGMVLPFLIGAAFSFSMHRSEDHLGQGTYILFLGVALSVTAFPVLARILAELK 189

Query: 196 LINSKFGRIALSSSMVSNFVSFFLLKLGALL--TTEARYKVLSTLYSFVVVVVVIIFVIR 255
           LIN++ GRI++S+++V++  ++ LL L   L  + +  +  L  + S  V + V +FV+R
Sbjct: 190 LINTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVMISSAVFIAVCVFVVR 249

Query: 256 PAILWMIKQHPVGQPLKEGFVITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIPSGPPLG 315
           P I W+I++ P G+   E  +  +L  V+ + F + A G H  FG  + G+ IP+G PLG
Sbjct: 250 PGIAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGLVIPNG-PLG 309

Query: 316 SALVERLDFITSWLFMPIFFVKIGLVVDIYAIE-LINFLCTSFIIFLSALGKFLGALLAS 375
             L+E+L+   S L +P+FF   GL  +I AI+    +L    +IFL+  GK +G ++ +
Sbjct: 310 LTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVIFLACAGKVIGTVIVA 369

Query: 376 IYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAAVIAPIL 435
            +  +P+R+ ++LGL+LN++G  E+ +  + K  K ++DETF    +  LV+  VI PI+
Sbjct: 370 FFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALVMTGVITPIV 429

Query: 436 RYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQEDVPSAINLLDALNPTRRSHLYVY 495
             L+ P ++ V YKRRT+  ++P+ +L VLVC+H   +VP+ INLL+A +PT+RS + +Y
Sbjct: 430 TILYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASHPTKRSPICIY 489

Query: 496 MLHLVELLGRATPQLIHHKLTK----VRTSRHSPSEPIVNAFKYFGQRNREIVKIYPFTA 555
           +LHLVEL GRA+  LI H   K          + S+ I+NAF+ + +++   V + P TA
Sbjct: 490 VLHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFENY-EQHAAFVAVQPLTA 549

Query: 556 ISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKNKMKMVNLHILDKAPCSVA 615
           ISP ++MH+DVCSLA +K+ S I++PFHK+   +G M  +    ++VN ++L+ +PCSV 
Sbjct: 550 ISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQNLLENSPCSVG 609

Query: 616 LVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINLTMIRLLEDM 675
           ++V+RG LN +  + +N    QV V+F GGPDDREA+    RM +HP I LT++R + D 
Sbjct: 610 ILVDRG-LNGATRLNSNTVSLQVAVLFFGGPDDREALAYAWRMAQHPGITLTVLRFIHDE 669

Query: 676 D----------------IPS-DNVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDSTGT 735
           D                IP  D+  +R LD++ +  F+   A    + YIE++V +   T
Sbjct: 670 DEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLVSNGEET 729

Query: 736 VSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMILVV 788
           V+ +RSM ++ DL +VGR     S +  GL  W E  ELG IG+ LASSDF     +LVV
Sbjct: 730 VAAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAATVSVLVV 789

BLAST of Lag0027469 vs. ExPASy Swiss-Prot
Match: Q9LMJ1 (Cation/H(+) antiporter 14 OS=Arabidopsis thaliana OX=3702 GN=CHX14 PE=2 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 7.6e-112
Identity = 251/776 (32.35%), Postives = 437/776 (56.31%), Query Frame = 0

Query: 20  LCTFAGRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLIVSQILGA 79
           +C     + S GVF G++PL++++PL+ LQ+ +   T  L  RLLKP  Q +I +Q+L  
Sbjct: 27  VCQKNHMLTSKGVFLGSDPLKYAMPLMLLQMSVIIITSRLLYRLLKPLKQGMISAQVLAG 86

Query: 80  LVLGSSGLCHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDFSIVKRIDKKAFG 139
           ++LG S       + +   P+ G I L  +S LG   + FL+G++ D SI+++   KA  
Sbjct: 87  IILGPSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHLFLLGLRIDASIIRKAGSKAIL 146

Query: 140 IGFCSVISP---SILTFIFPFTLINIVDSQTLRSLAIIGRAEVFINFTMVASLLSELHLI 199
           IG  S   P     LT +F     N+     +  ++ +       +F +  ++L+EL+++
Sbjct: 147 IGTASYALPFSLGNLTVLFLKNTYNL-PPDVVHCISTVISLNAMTSFPVTTTVLAELNIL 206

Query: 200 NSKFGRIALSSSMVSNFVSFFLLKLGALLTTEARYKVLSTLYSFV---VVVVVIIFVIRP 259
           NS  GR+A + S+V    S+ +  +  +   +     L++++SFV    +++VI FV RP
Sbjct: 207 NSDLGRLATNCSIVCEAFSWIVALVFRMFLRDG---TLASVWSFVWVTALILVIFFVCRP 266

Query: 260 AILWMIKQHPVGQPLKEGFV--ITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIPSGPPL 319
           AI+W+ ++  +    K G +    +++V+L  +  S+  G+H  FG   LGV++P GPPL
Sbjct: 267 AIIWLTERRSISID-KAGEIPFFPIIMVLLTISLTSEVLGVHAAFGAFWLGVSLPDGPPL 326

Query: 320 GSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELINFLCTSFIIFLSALGKFLGALLAS 379
           G+ L  +L+   + L +P F    GL  + + I   +      +I ++   KFLG   AS
Sbjct: 327 GTGLTTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKIIEAVILITYGCKFLGTAAAS 386

Query: 380 IYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAAVIAPIL 439
            Y  + + DA SL L++  QG  E+    M K +K +N E F +  I LL++  +   ++
Sbjct: 387 AYCNIQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNTECFNLLIITLLLVTGISRFLV 446

Query: 440 RYLFDPSRRYVVYKRRTVMHSRP-EFDLCVLVCIHDQEDVPSAINLLDALNPTRRSHLYV 499
             L+DPS+RY    +RT++ +R       +L+C+++ E+VPS +NLL+A  P+R S + V
Sbjct: 447 VCLYDPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVENVPSMVNLLEASYPSRFSPISV 506

Query: 500 YMLHLVELLGRATPQLI-HHKLTKVRTSRHSPSEPIVNAFKYFGQRNREIVKIYPFTAIS 559
           + LHLVEL GRA   L+ HH++ K+  +    S  IVN F+ F Q+N+  +    FTA +
Sbjct: 507 FTLHLVELKGRAHAVLVPHHQMNKLDPNT-VQSTHIVNGFQRFEQQNQGTLMAQHFTAAA 566

Query: 560 PPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKNKMKMVNLHILDKAPCSVALV 619
           P +S++DD+C+LA +KK +LI++PFHK++  +G +      ++ +NL++L+KAPCSV + 
Sbjct: 567 PFSSINDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNPSIRNINLNVLEKAPCSVGIF 626

Query: 620 VERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINLTMIRLLEDMDI 679
           ++RG     RS+  +     V V+FI G DD EA+    R+ EHP +++TMI       +
Sbjct: 627 IDRGETEGRRSVLMSYTWRNVAVIFIEGRDDAEALAFSMRIAEHPEVSVTMIHFRHKSSL 686

Query: 680 PSD---NVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDSTGTVSILRSMGNNFDLVMV 739
             +   +V   + ++  + +F+    +  ++ Y EE+V+D   T  ++ S+G++FDLV+V
Sbjct: 687 QQNHVVDVESELAESYLINDFKNFAMSKPKISYREEIVRDGVETTQVISSLGDSFDLVVV 746

Query: 740 GRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMILVVQQHTNVVDEDQW 783
           GR H   S ++ GL  W E  ELG+IG+  ASSDF  + +++  Q+  ++  ++ +
Sbjct: 747 GRDHDLESSVLYGLTDWSECPELGVIGDMFASSDFHFSVLVIHQQEGDSLAMDNSY 796

BLAST of Lag0027469 vs. ExPASy Swiss-Prot
Match: O22920 (Cation/H(+) symporter 13 OS=Arabidopsis thaliana OX=3702 GN=CHX13 PE=1 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 2.7e-109
Identity = 252/777 (32.43%), Postives = 432/777 (55.60%), Query Frame = 0

Query: 20  LCTFAGRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLIVSQILGA 79
           +C     + S G+F  +NPL++++PLL LQ+ +   T  L  R+L+P  Q +I +Q+L  
Sbjct: 29  VCQAQNMLTSRGIFMKSNPLKYALPLLLLQMSVIIVTSRLIFRVLQPLKQGMISAQVLTG 88

Query: 80  LVLGSSGLCHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDFSIVKRIDKKAFG 139
           +VLG S L H   +     P  G II+  +S +G V + FL+G++ D SI+++   KA  
Sbjct: 89  VVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHLFLLGLKIDGSIIRKAGSKAIL 148

Query: 140 IGFCSVISPSILTFIFPFTLINI----------VDSQTLRSLAIIGRAEVFINFTMVASL 199
           IG  S        + FPF+L N+          + S  +   +         +F +  ++
Sbjct: 149 IGTAS--------YAFPFSLGNLTIMFISKTMGLPSDVISCTSSAISLSSMTSFPVTTTV 208

Query: 200 LSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLTTEARYKVLSTLYSFVVVVVVIIF 259
           L+EL+++NS+ GR+A   SMV    S+F+     L T +     L  L   + +++VI F
Sbjct: 209 LAELNILNSELGRLATHCSMVCEVCSWFVALAFNLYTRDRTMTSLYALSMIIGLLLVIYF 268

Query: 260 VIRPAILWMIKQHPVGQPLKEGF-VITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIPSG 319
           V RP I+W+ ++       K+      +LL++  A+   +A G+H  FG   LGV++P G
Sbjct: 269 VFRPIIVWLTQRKTKSMDKKDVVPFFPVLLLLSIASLSGEAMGVHAAFGAFWLGVSLPDG 328

Query: 320 PPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAI---ELINFLCTSFIIFLSALGKFL 379
           PPLG+ L  +L+   S LF+P F    GL  + + I      + +    I+ ++   KFL
Sbjct: 329 PPLGTELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHHVVMIEIILLITYGCKFL 388

Query: 380 GALLASIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAA 439
           G   AS Y +  + DA+ L  ++  QG  E+    + K  + ++ E F +  I +L +  
Sbjct: 389 GTAAASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQVVDTECFNLVIITILFVTG 448

Query: 440 VIAPILRYLFDPSRRYVVYKRRTVMHSRP-EFDLCVLVCIHDQEDVPSAINLLDALNPTR 499
           +   ++ YL+DPS+RY    +RT++++R     L +L+ +++ E+VPS +NLL+A  PTR
Sbjct: 449 ISRFLVVYLYDPSKRYKSKSKRTILNTRQHNLQLRLLLGLYNVENVPSMVNLLEATYPTR 508

Query: 500 RSHLYVYMLHLVELLGRATPQLI-HHKLTKVRTSRHSPSEPIVNAFKYFGQRNREIVKIY 559
            + +  + LHLVEL GRA   L  HH++ K+  +  + S  IVNAF+ F Q+ +  +   
Sbjct: 509 FNPISFFTLHLVELKGRAHALLTPHHQMNKLDPNT-AQSTHIVNAFQRFEQKYQGALMAQ 568

Query: 560 PFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKNKMKMVNLHILDKAP 619
            FTA +P +S+++D+C+LA +KK +LI++PFHK++  +G +      ++ +NL++LD AP
Sbjct: 569 HFTAAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQVNGPIRTINLNVLDAAP 628

Query: 620 CSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINLTMIRL 679
           CSVA+ ++RG     RS+        V ++FIGG DD EA+ +  RM E P++N+TMI  
Sbjct: 629 CSVAIFIDRGETEGRRSVLMTNTWQNVAMLFIGGKDDAEALALCMRMAEKPDLNVTMIHF 688

Query: 680 LEDMDIPSDNVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDSTGTVSILRSMGNNFDL 739
                +  ++ ++ M +   + +F+   AN  ++ Y+EE+V+D   T  ++ S+G+ +D+
Sbjct: 689 RHKSALQDEDYSD-MSEYNLISDFKSYAANKGKIHYVEEIVRDGVETTQVISSLGDAYDM 748

Query: 740 VMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMILVVQQHTNVVDED 781
           V+VGR H   S ++ GL  W E  ELG+IG+ L S DF  + +++  QQ  +++  D
Sbjct: 749 VLVGRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDFHFSVLVVHQQQGDDLLAMD 795

BLAST of Lag0027469 vs. ExPASy Swiss-Prot
Match: Q9FFB8 (Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 8.7e-108
Identity = 244/710 (34.37%), Postives = 410/710 (57.75%), Query Frame = 0

Query: 104 IILDVVSALGHVFYFFLIGVQTDFSIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIV 163
           I+  + +A  ++ ++FL+GV+ D  +++   +KA  IG  SV+  +++  +  F  +  V
Sbjct: 117 IVFSLTAACSYMMFWFLMGVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDV 176

Query: 164 DSQ----TLRSL--AIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFF 223
            ++    TL SL   +I   +   +F +V +LL EL L NS+ GR+A+SS+++S+F +  
Sbjct: 177 GTKNSDHTLNSLEYVVIYSIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSI 236

Query: 224 L--------------LKLGALLTTE--ARYKVLSTLYSFVVVVVVIIFVIRPAILWMIKQ 283
           L               +LG++   +  A  + L      V+ V + I+V RP + ++IKQ
Sbjct: 237 LASVLIFMKELKDEQTRLGSVFIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQ 296

Query: 284 HPVGQPLKEGFVITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIPSGPPLGSALVERLDF 343
            P G+P+K  ++ T++++V  +A  +      I+ GP +LG+ +P GPPLGSA++++ + 
Sbjct: 297 TPSGRPVKAIYLSTIIVMVSGSAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYES 356

Query: 344 ITSWLFMPIFFVKIGLVVDIYAI-------ELINFLCTSFIIFLSALGKFLGALLASIYF 403
                F+P F       +DI A+        +I  + TSF++      KF+   + ++++
Sbjct: 357 AIFGTFLPFFIASSSTEIDISALFGWEGLNGIILIMVTSFVV------KFIFTTVPALFY 416

Query: 404 KLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAAVIAPILRYL 463
            +PM D  +L L+++ +G FELG Y +  +  ++  ETF VAC+++ + +A+I PILRYL
Sbjct: 417 GMPMEDCFALSLIMSFKGIFELGAYALAYQRGSVRPETFTVACLYITLNSAIIPPILRYL 476

Query: 464 FDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQEDVPSAINLLDALNPTRRSHLYVYMLH 523
           +DPSR Y  Y++R + H +P  +L +L CI+  +D+   INLL+A+ P+R S +  Y+LH
Sbjct: 477 YDPSRMYAGYEKRNMQHLKPNSELRILSCIYRTDDISPMINLLEAICPSRESPVATYVLH 536

Query: 524 LVELLGRATPQLIHHKLTKVRTSRHSPSEPIVNAFKYFGQRNREIVKIYPFTAISPPASM 583
           L+EL+G+A P  I HKL   RT   S S  ++ +F+ F +     V +  +TA+S P +M
Sbjct: 537 LMELVGQANPIFISHKLQTRRTEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTM 596

Query: 584 HDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKNKM-KMVNLHILDKAPCSVALVVER- 643
           H D+C LA    TSLIL+PFH+ + ++G   +S N M + +N  +LD APCSV + V R 
Sbjct: 597 HGDICMLALNNTTSLILLPFHQTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRS 656

Query: 644 --GILNVSRSIAT------NLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINLTMIRLL 703
             G  N+S    T      NL  + + ++F+GG DDREA+ +  RM   P IN+T++RL+
Sbjct: 657 SSGRKNISSGRKTINGTVPNLSSYNICMIFLGGKDDREAVTLATRMARDPRINITIVRLI 716

Query: 704 -EDMDIPSDNVNERMLDNEAVFEFQQIMANN-YRVRYIEEVVKDSTGTVSILRSMGNNFD 763
             D     + V ++MLD+E +   + + +N    + Y E+ ++D+  T S+LRSM ++FD
Sbjct: 717 TTDEKARENTVWDKMLDDELL---RDVKSNTLVDIFYSEKAIEDAAETSSLLRSMVSDFD 776

Query: 764 LVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMILVVQQ 773
           + +VGR +   SV  +GL  W E  ELGIIG+ L S DF     +LV+QQ
Sbjct: 777 MFIVGRGNGRTSVFTEGLEEWSEFKELGIIGDLLTSQDFNCQASVLVIQQ 817

BLAST of Lag0027469 vs. ExPASy Swiss-Prot
Match: Q9LUN4 (Cation/H(+) antiporter 19 OS=Arabidopsis thaliana OX=3702 GN=CHX19 PE=2 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 2.9e-103
Identity = 241/787 (30.62%), Postives = 424/787 (53.88%), Query Frame = 0

Query: 13  AFKNSTSLCTFAGRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLI 72
           A  N T  C    +  S G F   +PL+F++PL+ LQ+ +      L +  LKP  QP +
Sbjct: 2   ASTNVTGQCPGPMKATSNGAFQNESPLDFALPLIILQIVLVVVFTRLLAYFLKPLKQPRV 61

Query: 73  VSQILGALVLGSSGLCHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDFSIVKR 132
           +++I+G ++LG S L   + + + IFP +   +LD ++ +G +F+ FL+G++ DF+ +K+
Sbjct: 62  IAEIIGGILLGPSALGRSKAYLDTIFPKKSLTVLDTLANIGLLFFLFLVGLELDFAAIKK 121

Query: 133 IDKKAFGIGFCSVISPSIL----TFIFPFTLINIVDSQTLRSLAIIGRAEVFINFTMVAS 192
             KK+  I    +  P I+    +F+   T+   VD   L  +  +G A     F ++A 
Sbjct: 122 TGKKSLLIAIAGISLPFIVGVGTSFVLSATISKGVDQ--LPFIVFMGVALSITAFPVLAR 181

Query: 193 LLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLTTEARYKVLS--TLYSFVVVVVV 252
           +L+EL L+ +  GR+A+S++ V++  ++ LL L   L+ +    ++S   L      V+ 
Sbjct: 182 ILAELKLLTTDIGRMAMSAAGVNDVAAWILLALAIALSGDGTSPLVSVWVLLCGTGFVIF 241

Query: 253 IIFVIRPAILWMIKQHPVGQPLKEGFVITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIP 312
            +  I+P + +M ++ P G+P+KE +V   L VVLAA+F +   G+H  FG  ++G+  P
Sbjct: 242 AVVAIKPLLAYMARRCPEGEPVKELYVCVTLTVVLAASFVTDTIGIHALFGAFVVGIVAP 301

Query: 313 SGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIE-LINFLCTSFIIFLSALGKFL 372
              P    L E+++ + S L +P++F   GL  D+  I    ++     +I  +  GK +
Sbjct: 302 KEGPFCRILTEKIEDLVSGLLLPLYFAASGLKTDVTTIRGAQSWGLLVLVILTTCFGKIV 361

Query: 373 GALLASIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAA 432
           G + +S+  K+P R+AV+LG ++N++G  EL +  + K  K +ND+ F +  +  L    
Sbjct: 362 GTVGSSMLCKVPFREAVTLGFLMNTKGLVELIVLNIGKDRKVLNDQAFAILVLMALFTTF 421

Query: 433 VIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQEDVPSAINLLDALNPT-R 492
           +  PI+  ++ P+R+   YK RT+     + +L +L C H   ++P+ INL+++   T +
Sbjct: 422 ITTPIVMLIYKPARKGAPYKHRTIQRKDHDSELRILACFHSTRNIPTLINLIESSRGTGK 481

Query: 493 RSHLYVYMLHLVELLGRATPQLIHHKLTK----VRTSRHSPSEPIVNAFKYFGQRNREIV 552
           +  L VY +HL+EL  R++   + HK       +       ++ +V AF+ +  ++   V
Sbjct: 482 KGRLCVYAMHLMELSERSSAIAMVHKARNNGLPIWNKIERSTDQMVIAFEAY--QHLRAV 541

Query: 553 KIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKNKMKMVNLHILD 612
            + P TAIS  +S+H+D+C+ AH+K+ ++IL+PFHK    +G M    ++   VN  +L 
Sbjct: 542 AVRPMTAISGLSSIHEDICTSAHQKRVAMILLPFHKHQRMDGAMESIGHRFHEVNQRVLQ 601

Query: 613 KAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINLTM 672
           +APCSV ++V+RG+   S+ +A+ +  ++VV+ F GG DDREA+  G +MVEHP I LT+
Sbjct: 602 RAPCSVGILVDRGLGGTSQVVASEV-AYKVVIPFFGGLDDREALAYGMKMVEHPGITLTV 661

Query: 673 ---------IRLLEDMDIPSDNVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDSTGTV 732
                    ++  E  +       E+  D E V E       N  + Y E VV+     +
Sbjct: 662 YKFVAARGTLKRFEKSEHDEKEKKEKETDEEFVRELMNDPRGNESLAYEERVVESKDDII 721

Query: 733 SILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMILVVQ 779
           + L+SM +  +L +VGR     +  V  LV   +  ELG +G  L+SS+F     +LVVQ
Sbjct: 722 ATLKSM-SKCNLFVVGR-----NAAVASLVKSTDCPELGPVGRLLSSSEFSTTASVLVVQ 777

BLAST of Lag0027469 vs. ExPASy TrEMBL
Match: A0A6J1IZN7 (cation/H(+) antiporter 15-like OS=Cucurbita maxima OX=3661 GN=LOC111482115 PE=4 SV=1)

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 574/800 (71.75%), Postives = 672/800 (84.00%), Query Frame = 0

Query: 1   MGSIVMEPGDIAAF----------KNSTSLCTFAGRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSI+MEP D+AA+          KN T +CTFA RIHS GVF+G NPLEFSVPLLFLQL
Sbjct: 1   MGSIIMEPDDVAAYISGCDSHNTLKNITRICTFADRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLCHLEKFREAIFPLRGFIILDVVS 120
           G+ AGTIILFS LLKP GQPLIVSQILG +VLGSSGL HL +FRE IFPLRGF++LDV+S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGHVFYFFLIGVQTDFSIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDSQTLRS 180
           ALGHVF+FFLIGVQTD S VK+IDKKAF IG CSVI   IL+ I+  TL+NIVD QT++ 
Sbjct: 121 ALGHVFFFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLT--TE 240
           L  IG  E FINF MVASL+SELHLINS+FGR ALS++M SNF S  L  LGALLT   E
Sbjct: 181 LFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLTPQDE 240

Query: 241 ARYKVLSTLYSFVVVVVVIIFVIRPAILWMIKQHPVGQPLKEGFVITLLLVVLAAAFCSK 300
            +Y+ +STLY+ +++V VI F IRP I+WMIK++P+GQPLKE FV+TLLL+VL  AFC +
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELIN 360
           A GLHIY GPL LGVTIPSGPP+GSALV+RLDFITSW+FMPIFFVKIGL V+IYAI+LIN
Sbjct: 301 ALGLHIYTGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLAVNIYAIKLIN 360

Query: 361 FLCTSFIIFLSALGKFLGALLASIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAI 420
           FLC SFI+F++ALGKFLGALL S YFKL MRDAVSLGL+LN QGA ELG++K+M+K+K I
Sbjct: 361 FLCMSFIVFVNALGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQE 480
           NDE  VV C++++V+ A+I+PI+R+L DPSRR++V+KRRTVMHSRPEFDLCVLVCIHDQE
Sbjct: 421 NDEALVVMCVWVMVVVAIISPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCVLVCIHDQE 480

Query: 481 DVPSAINLLDALNPTRRSHLYVYMLHLVELLGRATPQLIHHKLTKVRTSRHSPSEPIVNA 540
           DVPSAINLLDALNPTRRSHL VY+LHLVELLGRA  +LI+HK  +VRTSR   S PIVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSK 600
           FKYFG+  REI+ IYPFTAISP  +MHD VCSLA +KKTSLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKREILAIYPFTAISPSTTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 NKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGA 660
            K KMVN HIL+ APCSVALVVERGIL   +SIATN HPFQ+V+VFIGGPDDREAMFIGA
Sbjct: 601 YKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIVMVFIGGPDDREAMFIGA 660

Query: 661 RMVEHPNINLTMIRLLEDMDIPSDNVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDST 720
           RMV HPNINLT IRL E  ++PS +V ER LDNE+V EF+Q +A+N RV+YIEEVVKD  
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEVVKDGI 720

Query: 721 GTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMIL 780
           GT+SILRS+G +FDLV+VGR+H+PC  +VQGLV W+EQTELG IGE LASSDF+GN  IL
Sbjct: 721 GTISILRSLGGDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTNVVDEDQWNHQETI 789
           VVQQH  V++ED+  +QE I
Sbjct: 781 VVQQHRRVINEDEELNQEDI 800

BLAST of Lag0027469 vs. ExPASy TrEMBL
Match: A0A6J1F2I8 (cation/H(+) antiporter 15-like OS=Cucurbita moschata OX=3662 GN=LOC111441553 PE=4 SV=1)

HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 571/800 (71.38%), Postives = 670/800 (83.75%), Query Frame = 0

Query: 1   MGSIVMEPGDIAAF----------KNSTSLCTFAGRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSI+MEP D+AA+          KN T +CTFA RIHS GVF+G NPLEFSVPLLFLQL
Sbjct: 1   MGSIIMEPDDVAAYISGGHGQNTLKNLTRICTFANRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLCHLEKFREAIFPLRGFIILDVVS 120
           G+ AGTIILFS LLKP GQPLIVSQILG +VLGSSGL HL +FRE IFPLRGF++LDV+S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGHVFYFFLIGVQTDFSIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDSQTLRS 180
           ALGHVFYFFLIGVQTD S VK+IDKKAF IG CSVI   IL+ I+  TL+NIVD QT++ 
Sbjct: 121 ALGHVFYFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLT--TE 240
           L  IG  E FINF MVASL+SELHLINS+FGR ALS++M SNF S  L  LGALL   +E
Sbjct: 181 LFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLAPQSE 240

Query: 241 ARYKVLSTLYSFVVVVVVIIFVIRPAILWMIKQHPVGQPLKEGFVITLLLVVLAAAFCSK 300
            +Y+ +STLY+ +++V VI F IRP I+WMIK++P+GQPLKE FV+TLLL+VL  AFC +
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELIN 360
           A GLHIY GPL LGVTIPSGPP+GSALV+RLDFITSW+FMPIFFVKIGLVV+IYA +LIN
Sbjct: 301 ASGLHIYIGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLVVNIYATKLIN 360

Query: 361 FLCTSFIIFLSALGKFLGALLASIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAI 420
           FLC SFI+F+SA+GKFLGALL S YFKL MRDAVSLGL+LN QGA ELG++K+M+K+K I
Sbjct: 361 FLCMSFIVFVSAMGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQE 480
           NDE  VV C++++V+ A+I PI+R+L DPSRR++V+KRRTVMHSRPEFDLC LVCIHDQE
Sbjct: 421 NDEALVVTCVWVMVVVAIITPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCALVCIHDQE 480

Query: 481 DVPSAINLLDALNPTRRSHLYVYMLHLVELLGRATPQLIHHKLTKVRTSRHSPSEPIVNA 540
           DVPSAINLLDALNPTRRSHL VY+LHLVELLGRA  +LI+HK  +VRTSR   S PIVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSK 600
           FKYFG+   EI+ IYPFTAISP ++MHD VCSLA +KKTSLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKSEILAIYPFTAISPSSTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 NKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGA 660
            K KMVN HIL+ APCSVALVVERGIL   +SIATN HPFQ+ +VFIGGPDDREAMFIGA
Sbjct: 601 YKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIAMVFIGGPDDREAMFIGA 660

Query: 661 RMVEHPNINLTMIRLLEDMDIPSDNVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDST 720
           RMV HPNINLT IRL E  ++PS +V ER LDNE+V EF+Q +A+N RV+YIEE+VKD  
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEMVKDGI 720

Query: 721 GTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMIL 780
           GT+SILRS+G++FDLV+VGR+H+PC  +VQGLV W+EQTELG IGE LASSDF+GN  IL
Sbjct: 721 GTISILRSLGSDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTNVVDEDQWNHQETI 789
           VVQQH  VV+ED+  +QE I
Sbjct: 781 VVQQHRRVVNEDEELNQEDI 800

BLAST of Lag0027469 vs. ExPASy TrEMBL
Match: A0A5D3DF05 (Cation/H(+) antiporter 15-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold318G00110 PE=4 SV=1)

HSP 1 Score: 1046.6 bits (2705), Expect = 5.3e-302
Identity = 547/801 (68.29%), Postives = 640/801 (79.90%), Query Frame = 0

Query: 1   MGSIVMEPGDIA----------AFKNSTSLCTFAGRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSIVMEP D+           AFKN +++CTFA RIH  GVF GANPLEFSV LL  QL
Sbjct: 1   MGSIVMEPEDVVAYMNGETGHNAFKNFSTICTFANRIHCSGVFNGANPLEFSVSLLLFQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLCHLEKFREAIFPLRGFIILDVVS 120
           GIC+GTIILFS+LLKP G PLIVSQILG LVLGSSGL HLEKF+E +FPLRGF+ LDVVS
Sbjct: 61  GICSGTIILFSQLLKPLGLPLIVSQILGGLVLGSSGLSHLEKFKEKVFPLRGFVCLDVVS 120

Query: 121 ALGHVFYFFLIGVQTDFSIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDSQTLRS 180
           ALGH+FYFFLIG+QTD SI+K ID KAFGIG CS I   +L   +   L NIVD + ++ 
Sbjct: 121 ALGHIFYFFLIGLQTDISILKEIDIKAFGIGSCSTIFAMLLISTYSLFLSNIVDVKKIQH 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLTTEAR 240
           +      E FI++ MV SLL ELHLINSKFGRI+LS+SM S+ +   L  L A+ +T   
Sbjct: 181 IFEFASLESFISYPMVVSLLYELHLINSKFGRISLSASMASSLLKICLTILAAIRSTNGE 240

Query: 241 YK--VLSTLYSFVVVVVVIIFVIRPAILWMIKQHPVGQPLKEGFVITLLLVVLAAAFCSK 300
            K  V + ++S VV++++II+VIRPA LWM K++P+GQPLKE FVITLLL VL  AFC +
Sbjct: 241 TKDLVSTKIFSIVVLILLIIYVIRPATLWMAKENPIGQPLKEYFVITLLLGVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVD-IYAIELI 360
            FGL IYFG  LLG  IPS PP+GS LV+RL+FITSW+FMPIFFV+IGLV+D IY I L+
Sbjct: 301 TFGLRIYFGSFLLGFIIPSDPPIGSTLVDRLEFITSWIFMPIFFVRIGLVIDNIYTITLV 360

Query: 361 NFLCTSFIIFLSALGKFLGALLASIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKA 420
           N L  SFIIF+SALGKFLG+L+ S+Y+K PMRDA+SLGL+LNSQGA EL M+K+MKKDK 
Sbjct: 361 NLLSMSFIIFISALGKFLGSLIISMYYKFPMRDAISLGLILNSQGALELSMFKIMKKDKL 420

Query: 421 INDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQ 480
           I+DE FVV CI ++V+ A+I PI+RYL  PS+RY+V+KRRTVMHSRPEFDLCVLVCIHDQ
Sbjct: 421 IDDEAFVVGCISIMVIVAIITPIIRYLLRPSKRYIVHKRRTVMHSRPEFDLCVLVCIHDQ 480

Query: 481 EDVPSAINLLDALNPTRRSHLYVYMLHLVELLGRATPQLIHHKLTKVRTSRHSPSEPIVN 540
           EDVPS INLLDALNPTRRSHL VYMLHLVELLGRA P+LIHHK   VR+ R S S+PIVN
Sbjct: 481 EDVPSVINLLDALNPTRRSHLIVYMLHLVELLGRAQPKLIHHKHKMVRSLRSSSSKPIVN 540

Query: 541 AFKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLS 600
           AFKYF      IV I  FTAISP  +MHDDVCSLA +K TSLIL PFHKR+HSNG++S S
Sbjct: 541 AFKYFEDSKSNIVAINLFTAISPSTTMHDDVCSLALDKSTSLILAPFHKRYHSNGMVSFS 600

Query: 601 KNKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIG 660
           K+K+K+VN HILDKAPCSV L+VERG+L VS+SI TNL  FQ+VV+FIGGPDDREAMFIG
Sbjct: 601 KHKLKIVNHHILDKAPCSVGLIVERGLLRVSKSIETNLQSFQIVVIFIGGPDDREAMFIG 660

Query: 661 ARMVEHPNINLTMIRLLEDMDIPSDNVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDS 720
           ARMV H NINLTMIRLL++ ++P D+  E+ LD+EAV EF+QI+ANNYRVRY EEVVKD 
Sbjct: 661 ARMVGHVNINLTMIRLLDNENVPKDDFKEKRLDDEAVAEFRQIIANNYRVRYKEEVVKDG 720

Query: 721 TGTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMI 780
           T T+SILRSMG+NFDL+MVGRRHSP    VQGLV WDE+TELG IGE LASSDFMGN  I
Sbjct: 721 TETISILRSMGSNFDLIMVGRRHSPFLSPVQGLVLWDERTELGAIGEVLASSDFMGNATI 780

Query: 781 LVVQQHTNVVDEDQWNHQETI 789
           LVVQQHT V +ED  N  ETI
Sbjct: 781 LVVQQHTKVANEDHENPPETI 801

BLAST of Lag0027469 vs. ExPASy TrEMBL
Match: A0A1S3BPK0 (cation/H(+) antiporter 15-like OS=Cucumis melo OX=3656 GN=LOC103491816 PE=4 SV=1)

HSP 1 Score: 1041.6 bits (2692), Expect = 1.7e-300
Identity = 546/801 (68.16%), Postives = 637/801 (79.53%), Query Frame = 0

Query: 1   MGSIVMEPGDIA----------AFKNSTSLCTFAGRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSIVMEP D+           AFKN +++CTFA RIH  GVF GANPLEFSV LL  QL
Sbjct: 1   MGSIVMEPEDVVAYMNGETGHNAFKNFSTICTFANRIHCSGVFNGANPLEFSVSLLLFQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLCHLEKFREAIFPLRGFIILDVVS 120
           GIC+GTIILFS+LLKP G PLIVSQILG LVLGSSGL HLEKF+E +FPLRGF+ LDVVS
Sbjct: 61  GICSGTIILFSQLLKPLGLPLIVSQILGGLVLGSSGLSHLEKFKEKVFPLRGFVCLDVVS 120

Query: 121 ALGHVFYFFLIGVQTDFSIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDSQTLRS 180
           ALGH+FYFFLIG+QTD SI+K ID KAFGIG CS I   +L   +   L NIVD + ++ 
Sbjct: 121 ALGHIFYFFLIGLQTDISILKEIDIKAFGIGSCSTIFAMLLISTYSLFLSNIVDVKKIQH 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLTTEAR 240
           +      E FI++ MV SLL ELHLINSKFGRI+LS+SM S+ +   L  L A+ +T   
Sbjct: 181 IFEFASLESFISYPMVVSLLYELHLINSKFGRISLSASMASSLLKICLTILAAIRSTNGE 240

Query: 241 YK--VLSTLYSFVVVVVVIIFVIRPAILWMIKQHPVGQPLKEGFVITLLLVVLAAAFCSK 300
            K  V +  +S VV++++II+VIRPA LWM K++P+GQPLKE FVITLLL VL  AFC +
Sbjct: 241 TKDLVSTKNFSIVVLILLIIYVIRPATLWMAKENPIGQPLKEYFVITLLLGVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVD-IYAIELI 360
            FGL IYFG  LLG  IPS PP+GS LV+RL+FITSW+FMPIFFV+IGLV+D IY I L+
Sbjct: 301 TFGLRIYFGSFLLGFIIPSDPPIGSTLVDRLEFITSWIFMPIFFVRIGLVIDNIYTITLV 360

Query: 361 NFLCTSFIIFLSALGKFLGALLASIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKA 420
           N L  SFIIF+SALGKFLG+L+ S+Y+K PMRDA+SLGL+LNSQGA EL M+K+MKKDK 
Sbjct: 361 NLLSMSFIIFISALGKFLGSLIISMYYKFPMRDAISLGLILNSQGALELSMFKIMKKDKL 420

Query: 421 INDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQ 480
           I+DE FVV CI ++V+ A+I PI+RYL  PS+RY+V+KRRTVMHSRPEFDLCVLVCIHDQ
Sbjct: 421 IDDEAFVVGCISIMVIVAIITPIIRYLLRPSKRYIVHKRRTVMHSRPEFDLCVLVCIHDQ 480

Query: 481 EDVPSAINLLDALNPTRRSHLYVYMLHLVELLGRATPQLIHHKLTKVRTSRHSPSEPIVN 540
           EDVPS INLLDALNPTRRSHL VYMLHLVELLGRA P+LIHHK   VR+ R S SEPIVN
Sbjct: 481 EDVPSVINLLDALNPTRRSHLIVYMLHLVELLGRAQPKLIHHKHKMVRSLRSSSSEPIVN 540

Query: 541 AFKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLS 600
           AFKYF      IV I  FTAISP  +MHDDVCSLA +K TSLIL PFHKR+HSNG++S S
Sbjct: 541 AFKYFEDSKSNIVAINLFTAISPSTTMHDDVCSLALDKSTSLILAPFHKRYHSNGMVSFS 600

Query: 601 KNKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIG 660
           K+K+K+VN HILDKAPCSV L+VERG+L VS+SI TNL  FQ+VV+FIGG DDRE MFIG
Sbjct: 601 KHKLKIVNHHILDKAPCSVGLIVERGLLRVSKSIETNLQSFQIVVIFIGGADDRETMFIG 660

Query: 661 ARMVEHPNINLTMIRLLEDMDIPSDNVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDS 720
           ARMV H NINLTMIRLL++ ++P D+  E+ LD+EAV EF+QI+ANNYRVRY EEVVKD 
Sbjct: 661 ARMVGHVNINLTMIRLLDNENVPKDDFKEKRLDDEAVAEFRQIIANNYRVRYKEEVVKDG 720

Query: 721 TGTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMI 780
           T T+SILRSMG+NFDL+MVGRRHSP    VQGLV WDE+TELG IGE LASSDFMGN  I
Sbjct: 721 TETISILRSMGSNFDLIMVGRRHSPFLSPVQGLVLWDERTELGAIGEVLASSDFMGNATI 780

Query: 781 LVVQQHTNVVDEDQWNHQETI 789
           LVVQQHT V +ED  N  ETI
Sbjct: 781 LVVQQHTRVANEDHENPPETI 801

BLAST of Lag0027469 vs. ExPASy TrEMBL
Match: A0A6J1EN94 (cation/H(+) antiporter 15-like OS=Cucurbita moschata OX=3662 GN=LOC111435106 PE=4 SV=1)

HSP 1 Score: 1040.0 bits (2688), Expect = 5.0e-300
Identity = 528/783 (67.43%), Postives = 636/783 (81.23%), Query Frame = 0

Query: 1   MGSIVMEPGDIAAFKNSTSLCTFAGRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILF 60
           MGSIVMEPGD + F N +S+C    RIHS  VF G NPLEFSVPLL LQLGIC+GTII  
Sbjct: 1   MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL 60

Query: 61  SRLLKPFGQPLIVSQILGALVLGSSGLCHLEKFREAIFPLRGFIILDVVSALGHVFYFFL 120
            +LLK   QPLIVSQIL  LVLG  G   LEKFRE IFP+RG I LD +SALG VFYFFL
Sbjct: 61  FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL 120

Query: 121 IGVQTDFSIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDSQTLRSLAIIGRAEVF 180
           IGVQTD SI+K IDK+AFGIG CSVI P+IL+ ++  TL+N VD  T+++L ++G AE F
Sbjct: 121 IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF 180

Query: 181 INFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLTTE--ARYKVLSTLY 240
           I+F MVASLL+ELHLINSKFG+IAL+SSM SN    F  K+G LL  E   R   LST+Y
Sbjct: 181 IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY 240

Query: 241 SFVVVVVVIIFVIRPAILWMIKQHPVGQPLKEGFVITLLLVVLAAAFCSKAFGLHIYFGP 300
            F+V+ V+I+   +P ILWM K  PVGQPLKE FVITL L VL  AFCS+A G+HIYFGP
Sbjct: 241 GFIVLAVIIL-SFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGP 300

Query: 301 LLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELINFLCTSFIIFL 360
           +LLG+TIP GPP+GSA+VE+LD I SW+ MPIFF KIGL+  I+ I+L+NFLC SFII +
Sbjct: 301 ILLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIV 360

Query: 361 SALGKFLGALLASIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACI 420
           +A GKFLGAL+ SIY+KLP RD V+LGL+LNSQGA E+G++K+MKKDK +++ETFVV CI
Sbjct: 361 AAFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLMKKDKVLDEETFVVMCI 420

Query: 421 FLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQEDVPSAINLLD 480
            ++++AAVI PILRYLFDPSRR+V  K+RTVMH RPEFDL VLVCIHDQ+DVPSAIN+L+
Sbjct: 421 CVMLVAAVITPILRYLFDPSRRFVTEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILE 480

Query: 481 ALNPTRRSHLYVYMLHLVELLGRATPQLIHHKLTKVRTSRHS-PSEPIVNAFKYFGQRNR 540
           AL PT RSHL VYMLHLV LLGRA PQLIHH++TK+RTS  S  SE IVNAF YFGQ N 
Sbjct: 481 ALKPTHRSHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSYVSESIVNAFNYFGQSNS 540

Query: 541 EIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKNKMKMVNLH 600
           ++V I+PFTA+SP  +M++DVCSLA +K+TSLIL+PFHKRFHSNG+MSLSK KMKM+N H
Sbjct: 541 DVVTIHPFTAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQH 600

Query: 601 ILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNIN 660
           ILDKAPCSVAL+VERGI+ +SRSI+TNL+ FQ+ +VFIGGPDDREA+FIG RM+ HP IN
Sbjct: 601 ILDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYIN 660

Query: 661 LTMIRLLEDMDIPSDNVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDSTGTVSILRSM 720
           +T+IRLLED + PSD   E  L+NEAV ++ ++M NN+RVRY+EEV KD TGTV+ILR++
Sbjct: 661 VTLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILRTL 720

Query: 721 GNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMILVVQQHTNVV 780
            NNFDL++VGRRHSP S +VQGL+ W+EQTELG IGE LASSDF+GN  I VVQQH  VV
Sbjct: 721 DNNFDLILVGRRHSPRSPLVQGLLLWNEQTELGSIGEVLASSDFIGNATIFVVQQHRKVV 780

BLAST of Lag0027469 vs. TAIR 10
Match: AT2G13620.1 (cation/hydrogen exchanger 15 )

HSP 1 Score: 493.4 bits (1269), Expect = 3.3e-139
Identity = 291/798 (36.47%), Postives = 471/798 (59.02%), Query Frame = 0

Query: 16  NSTSLCTFAGRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLIVSQ 75
           +++ +C     I + GV+ G NPL+FS+PL  LQL +       F  +LKPF QP ++S+
Sbjct: 10  DASIICYAPSMITTNGVWQGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQPRVISE 69

Query: 76  ILGALVLGSSGLCHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDFSIVKRIDK 135
           ILG +VLG S L    KF   IFP R  ++L+ ++ +G +++ FL+GV+ D  +V++  K
Sbjct: 70  ILGGIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMVVRKTGK 129

Query: 136 KAFGIGFCSVISPSILTFIFPFTLINIVDSQTLRS-LAIIGRAEVFINFTMVASLLSELH 195
           +A  I    ++ P ++   F F++    D     + +  +G A     F ++A +L+EL 
Sbjct: 130 RALTIAIGGMVLPFLIGAAFSFSMHRSEDHLGQGTYILFLGVALSVTAFPVLARILAELK 189

Query: 196 LINSKFGRIALSSSMVSNFVSFFLLKLGALL--TTEARYKVLSTLYSFVVVVVVIIFVIR 255
           LIN++ GRI++S+++V++  ++ LL L   L  + +  +  L  + S  V + V +FV+R
Sbjct: 190 LINTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVMISSAVFIAVCVFVVR 249

Query: 256 PAILWMIKQHPVGQPLKEGFVITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIPSGPPLG 315
           P I W+I++ P G+   E  +  +L  V+ + F + A G H  FG  + G+ IP+G PLG
Sbjct: 250 PGIAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGLVIPNG-PLG 309

Query: 316 SALVERLDFITSWLFMPIFFVKIGLVVDIYAIE-LINFLCTSFIIFLSALGKFLGALLAS 375
             L+E+L+   S L +P+FF   GL  +I AI+    +L    +IFL+  GK +G ++ +
Sbjct: 310 LTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVIFLACAGKVIGTVIVA 369

Query: 376 IYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAAVIAPIL 435
            +  +P+R+ ++LGL+LN++G  E+ +  + K  K ++DETF    +  LV+  VI PI+
Sbjct: 370 FFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALVMTGVITPIV 429

Query: 436 RYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQEDVPSAINLLDALNPTRRSHLYVY 495
             L+ P ++ V YKRRT+  ++P+ +L VLVC+H   +VP+ INLL+A +PT+RS + +Y
Sbjct: 430 TILYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASHPTKRSPICIY 489

Query: 496 MLHLVELLGRATPQLIHHKLTK----VRTSRHSPSEPIVNAFKYFGQRNREIVKIYPFTA 555
           +LHLVEL GRA+  LI H   K          + S+ I+NAF+ + +++   V + P TA
Sbjct: 490 VLHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFENY-EQHAAFVAVQPLTA 549

Query: 556 ISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKNKMKMVNLHILDKAPCSVA 615
           ISP ++MH+DVCSLA +K+ S I++PFHK+   +G M  +    ++VN ++L+ +PCSV 
Sbjct: 550 ISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQNLLENSPCSVG 609

Query: 616 LVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINLTMIRLLEDM 675
           ++V+RG LN +  + +N    QV V+F GGPDDREA+    RM +HP I LT++R + D 
Sbjct: 610 ILVDRG-LNGATRLNSNTVSLQVAVLFFGGPDDREALAYAWRMAQHPGITLTVLRFIHDE 669

Query: 676 D----------------IPS-DNVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDSTGT 735
           D                IP  D+  +R LD++ +  F+   A    + YIE++V +   T
Sbjct: 670 DEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLVSNGEET 729

Query: 736 VSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMILVV 788
           V+ +RSM ++ DL +VGR     S +  GL  W E  ELG IG+ LASSDF     +LVV
Sbjct: 730 VAAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAATVSVLVV 789

BLAST of Lag0027469 vs. TAIR 10
Match: AT1G06970.1 (cation/hydrogen exchanger 14 )

HSP 1 Score: 406.4 bits (1043), Expect = 5.4e-113
Identity = 251/776 (32.35%), Postives = 437/776 (56.31%), Query Frame = 0

Query: 20  LCTFAGRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLIVSQILGA 79
           +C     + S GVF G++PL++++PL+ LQ+ +   T  L  RLLKP  Q +I +Q+L  
Sbjct: 27  VCQKNHMLTSKGVFLGSDPLKYAMPLMLLQMSVIIITSRLLYRLLKPLKQGMISAQVLAG 86

Query: 80  LVLGSSGLCHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDFSIVKRIDKKAFG 139
           ++LG S       + +   P+ G I L  +S LG   + FL+G++ D SI+++   KA  
Sbjct: 87  IILGPSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHLFLLGLRIDASIIRKAGSKAIL 146

Query: 140 IGFCSVISP---SILTFIFPFTLINIVDSQTLRSLAIIGRAEVFINFTMVASLLSELHLI 199
           IG  S   P     LT +F     N+     +  ++ +       +F +  ++L+EL+++
Sbjct: 147 IGTASYALPFSLGNLTVLFLKNTYNL-PPDVVHCISTVISLNAMTSFPVTTTVLAELNIL 206

Query: 200 NSKFGRIALSSSMVSNFVSFFLLKLGALLTTEARYKVLSTLYSFV---VVVVVIIFVIRP 259
           NS  GR+A + S+V    S+ +  +  +   +     L++++SFV    +++VI FV RP
Sbjct: 207 NSDLGRLATNCSIVCEAFSWIVALVFRMFLRDG---TLASVWSFVWVTALILVIFFVCRP 266

Query: 260 AILWMIKQHPVGQPLKEGFV--ITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIPSGPPL 319
           AI+W+ ++  +    K G +    +++V+L  +  S+  G+H  FG   LGV++P GPPL
Sbjct: 267 AIIWLTERRSISID-KAGEIPFFPIIMVLLTISLTSEVLGVHAAFGAFWLGVSLPDGPPL 326

Query: 320 GSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELINFLCTSFIIFLSALGKFLGALLAS 379
           G+ L  +L+   + L +P F    GL  + + I   +      +I ++   KFLG   AS
Sbjct: 327 GTGLTTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKIIEAVILITYGCKFLGTAAAS 386

Query: 380 IYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAAVIAPIL 439
            Y  + + DA SL L++  QG  E+    M K +K +N E F +  I LL++  +   ++
Sbjct: 387 AYCNIQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNTECFNLLIITLLLVTGISRFLV 446

Query: 440 RYLFDPSRRYVVYKRRTVMHSRP-EFDLCVLVCIHDQEDVPSAINLLDALNPTRRSHLYV 499
             L+DPS+RY    +RT++ +R       +L+C+++ E+VPS +NLL+A  P+R S + V
Sbjct: 447 VCLYDPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVENVPSMVNLLEASYPSRFSPISV 506

Query: 500 YMLHLVELLGRATPQLI-HHKLTKVRTSRHSPSEPIVNAFKYFGQRNREIVKIYPFTAIS 559
           + LHLVEL GRA   L+ HH++ K+  +    S  IVN F+ F Q+N+  +    FTA +
Sbjct: 507 FTLHLVELKGRAHAVLVPHHQMNKLDPNT-VQSTHIVNGFQRFEQQNQGTLMAQHFTAAA 566

Query: 560 PPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKNKMKMVNLHILDKAPCSVALV 619
           P +S++DD+C+LA +KK +LI++PFHK++  +G +      ++ +NL++L+KAPCSV + 
Sbjct: 567 PFSSINDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNPSIRNINLNVLEKAPCSVGIF 626

Query: 620 VERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINLTMIRLLEDMDI 679
           ++RG     RS+  +     V V+FI G DD EA+    R+ EHP +++TMI       +
Sbjct: 627 IDRGETEGRRSVLMSYTWRNVAVIFIEGRDDAEALAFSMRIAEHPEVSVTMIHFRHKSSL 686

Query: 680 PSD---NVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDSTGTVSILRSMGNNFDLVMV 739
             +   +V   + ++  + +F+    +  ++ Y EE+V+D   T  ++ S+G++FDLV+V
Sbjct: 687 QQNHVVDVESELAESYLINDFKNFAMSKPKISYREEIVRDGVETTQVISSLGDSFDLVVV 746

Query: 740 GRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMILVVQQHTNVVDEDQW 783
           GR H   S ++ GL  W E  ELG+IG+  ASSDF  + +++  Q+  ++  ++ +
Sbjct: 747 GRDHDLESSVLYGLTDWSECPELGVIGDMFASSDFHFSVLVIHQQEGDSLAMDNSY 796

BLAST of Lag0027469 vs. TAIR 10
Match: AT2G30240.1 (Cation/hydrogen exchanger family protein )

HSP 1 Score: 397.9 bits (1021), Expect = 1.9e-110
Identity = 252/777 (32.43%), Postives = 432/777 (55.60%), Query Frame = 0

Query: 20  LCTFAGRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLIVSQILGA 79
           +C     + S G+F  +NPL++++PLL LQ+ +   T  L  R+L+P  Q +I +Q+L  
Sbjct: 29  VCQAQNMLTSRGIFMKSNPLKYALPLLLLQMSVIIVTSRLIFRVLQPLKQGMISAQVLTG 88

Query: 80  LVLGSSGLCHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDFSIVKRIDKKAFG 139
           +VLG S L H   +     P  G II+  +S +G V + FL+G++ D SI+++   KA  
Sbjct: 89  VVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHLFLLGLKIDGSIIRKAGSKAIL 148

Query: 140 IGFCSVISPSILTFIFPFTLINI----------VDSQTLRSLAIIGRAEVFINFTMVASL 199
           IG  S        + FPF+L N+          + S  +   +         +F +  ++
Sbjct: 149 IGTAS--------YAFPFSLGNLTIMFISKTMGLPSDVISCTSSAISLSSMTSFPVTTTV 208

Query: 200 LSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLTTEARYKVLSTLYSFVVVVVVIIF 259
           L+EL+++NS+ GR+A   SMV    S+F+     L T +     L  L   + +++VI F
Sbjct: 209 LAELNILNSELGRLATHCSMVCEVCSWFVALAFNLYTRDRTMTSLYALSMIIGLLLVIYF 268

Query: 260 VIRPAILWMIKQHPVGQPLKEGF-VITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIPSG 319
           V RP I+W+ ++       K+      +LL++  A+   +A G+H  FG   LGV++P G
Sbjct: 269 VFRPIIVWLTQRKTKSMDKKDVVPFFPVLLLLSIASLSGEAMGVHAAFGAFWLGVSLPDG 328

Query: 320 PPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAI---ELINFLCTSFIIFLSALGKFL 379
           PPLG+ L  +L+   S LF+P F    GL  + + I      + +    I+ ++   KFL
Sbjct: 329 PPLGTELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHHVVMIEIILLITYGCKFL 388

Query: 380 GALLASIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAA 439
           G   AS Y +  + DA+ L  ++  QG  E+    + K  + ++ E F +  I +L +  
Sbjct: 389 GTAAASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQVVDTECFNLVIITILFVTG 448

Query: 440 VIAPILRYLFDPSRRYVVYKRRTVMHSRP-EFDLCVLVCIHDQEDVPSAINLLDALNPTR 499
           +   ++ YL+DPS+RY    +RT++++R     L +L+ +++ E+VPS +NLL+A  PTR
Sbjct: 449 ISRFLVVYLYDPSKRYKSKSKRTILNTRQHNLQLRLLLGLYNVENVPSMVNLLEATYPTR 508

Query: 500 RSHLYVYMLHLVELLGRATPQLI-HHKLTKVRTSRHSPSEPIVNAFKYFGQRNREIVKIY 559
            + +  + LHLVEL GRA   L  HH++ K+  +  + S  IVNAF+ F Q+ +  +   
Sbjct: 509 FNPISFFTLHLVELKGRAHALLTPHHQMNKLDPNT-AQSTHIVNAFQRFEQKYQGALMAQ 568

Query: 560 PFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKNKMKMVNLHILDKAP 619
            FTA +P +S+++D+C+LA +KK +LI++PFHK++  +G +      ++ +NL++LD AP
Sbjct: 569 HFTAAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQVNGPIRTINLNVLDAAP 628

Query: 620 CSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINLTMIRL 679
           CSVA+ ++RG     RS+        V ++FIGG DD EA+ +  RM E P++N+TMI  
Sbjct: 629 CSVAIFIDRGETEGRRSVLMTNTWQNVAMLFIGGKDDAEALALCMRMAEKPDLNVTMIHF 688

Query: 680 LEDMDIPSDNVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDSTGTVSILRSMGNNFDL 739
                +  ++ ++ M +   + +F+   AN  ++ Y+EE+V+D   T  ++ S+G+ +D+
Sbjct: 689 RHKSALQDEDYSD-MSEYNLISDFKSYAANKGKIHYVEEIVRDGVETTQVISSLGDAYDM 748

Query: 740 VMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMILVVQQHTNVVDED 781
           V+VGR H   S ++ GL  W E  ELG+IG+ L S DF  + +++  QQ  +++  D
Sbjct: 749 VLVGRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDFHFSVLVVHQQQGDDLLAMD 795

BLAST of Lag0027469 vs. TAIR 10
Match: AT5G22900.1 (cation/H+ exchanger 3 )

HSP 1 Score: 392.9 bits (1008), Expect = 6.1e-109
Identity = 244/710 (34.37%), Postives = 410/710 (57.75%), Query Frame = 0

Query: 104 IILDVVSALGHVFYFFLIGVQTDFSIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIV 163
           I+  + +A  ++ ++FL+GV+ D  +++   +KA  IG  SV+  +++  +  F  +  V
Sbjct: 117 IVFSLTAACSYMMFWFLMGVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDV 176

Query: 164 DSQ----TLRSL--AIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFF 223
            ++    TL SL   +I   +   +F +V +LL EL L NS+ GR+A+SS+++S+F +  
Sbjct: 177 GTKNSDHTLNSLEYVVIYSIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSI 236

Query: 224 L--------------LKLGALLTTE--ARYKVLSTLYSFVVVVVVIIFVIRPAILWMIKQ 283
           L               +LG++   +  A  + L      V+ V + I+V RP + ++IKQ
Sbjct: 237 LASVLIFMKELKDEQTRLGSVFIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQ 296

Query: 284 HPVGQPLKEGFVITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIPSGPPLGSALVERLDF 343
            P G+P+K  ++ T++++V  +A  +      I+ GP +LG+ +P GPPLGSA++++ + 
Sbjct: 297 TPSGRPVKAIYLSTIIVMVSGSAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYES 356

Query: 344 ITSWLFMPIFFVKIGLVVDIYAI-------ELINFLCTSFIIFLSALGKFLGALLASIYF 403
                F+P F       +DI A+        +I  + TSF++      KF+   + ++++
Sbjct: 357 AIFGTFLPFFIASSSTEIDISALFGWEGLNGIILIMVTSFVV------KFIFTTVPALFY 416

Query: 404 KLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAAVIAPILRYL 463
            +PM D  +L L+++ +G FELG Y +  +  ++  ETF VAC+++ + +A+I PILRYL
Sbjct: 417 GMPMEDCFALSLIMSFKGIFELGAYALAYQRGSVRPETFTVACLYITLNSAIIPPILRYL 476

Query: 464 FDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQEDVPSAINLLDALNPTRRSHLYVYMLH 523
           +DPSR Y  Y++R + H +P  +L +L CI+  +D+   INLL+A+ P+R S +  Y+LH
Sbjct: 477 YDPSRMYAGYEKRNMQHLKPNSELRILSCIYRTDDISPMINLLEAICPSRESPVATYVLH 536

Query: 524 LVELLGRATPQLIHHKLTKVRTSRHSPSEPIVNAFKYFGQRNREIVKIYPFTAISPPASM 583
           L+EL+G+A P  I HKL   RT   S S  ++ +F+ F +     V +  +TA+S P +M
Sbjct: 537 LMELVGQANPIFISHKLQTRRTEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTM 596

Query: 584 HDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKNKM-KMVNLHILDKAPCSVALVVER- 643
           H D+C LA    TSLIL+PFH+ + ++G   +S N M + +N  +LD APCSV + V R 
Sbjct: 597 HGDICMLALNNTTSLILLPFHQTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRS 656

Query: 644 --GILNVSRSIAT------NLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINLTMIRLL 703
             G  N+S    T      NL  + + ++F+GG DDREA+ +  RM   P IN+T++RL+
Sbjct: 657 SSGRKNISSGRKTINGTVPNLSSYNICMIFLGGKDDREAVTLATRMARDPRINITIVRLI 716

Query: 704 -EDMDIPSDNVNERMLDNEAVFEFQQIMANN-YRVRYIEEVVKDSTGTVSILRSMGNNFD 763
             D     + V ++MLD+E +   + + +N    + Y E+ ++D+  T S+LRSM ++FD
Sbjct: 717 TTDEKARENTVWDKMLDDELL---RDVKSNTLVDIFYSEKAIEDAAETSSLLRSMVSDFD 776

Query: 764 LVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMILVVQQ 773
           + +VGR +   SV  +GL  W E  ELGIIG+ L S DF     +LV+QQ
Sbjct: 777 MFIVGRGNGRTSVFTEGLEEWSEFKELGIIGDLLTSQDFNCQASVLVIQQ 817

BLAST of Lag0027469 vs. TAIR 10
Match: AT3G17630.1 (cation/H+ exchanger 19 )

HSP 1 Score: 377.9 bits (969), Expect = 2.0e-104
Identity = 241/787 (30.62%), Postives = 424/787 (53.88%), Query Frame = 0

Query: 13  AFKNSTSLCTFAGRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLI 72
           A  N T  C    +  S G F   +PL+F++PL+ LQ+ +      L +  LKP  QP +
Sbjct: 2   ASTNVTGQCPGPMKATSNGAFQNESPLDFALPLIILQIVLVVVFTRLLAYFLKPLKQPRV 61

Query: 73  VSQILGALVLGSSGLCHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDFSIVKR 132
           +++I+G ++LG S L   + + + IFP +   +LD ++ +G +F+ FL+G++ DF+ +K+
Sbjct: 62  IAEIIGGILLGPSALGRSKAYLDTIFPKKSLTVLDTLANIGLLFFLFLVGLELDFAAIKK 121

Query: 133 IDKKAFGIGFCSVISPSIL----TFIFPFTLINIVDSQTLRSLAIIGRAEVFINFTMVAS 192
             KK+  I    +  P I+    +F+   T+   VD   L  +  +G A     F ++A 
Sbjct: 122 TGKKSLLIAIAGISLPFIVGVGTSFVLSATISKGVDQ--LPFIVFMGVALSITAFPVLAR 181

Query: 193 LLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLTTEARYKVLS--TLYSFVVVVVV 252
           +L+EL L+ +  GR+A+S++ V++  ++ LL L   L+ +    ++S   L      V+ 
Sbjct: 182 ILAELKLLTTDIGRMAMSAAGVNDVAAWILLALAIALSGDGTSPLVSVWVLLCGTGFVIF 241

Query: 253 IIFVIRPAILWMIKQHPVGQPLKEGFVITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIP 312
            +  I+P + +M ++ P G+P+KE +V   L VVLAA+F +   G+H  FG  ++G+  P
Sbjct: 242 AVVAIKPLLAYMARRCPEGEPVKELYVCVTLTVVLAASFVTDTIGIHALFGAFVVGIVAP 301

Query: 313 SGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIE-LINFLCTSFIIFLSALGKFL 372
              P    L E+++ + S L +P++F   GL  D+  I    ++     +I  +  GK +
Sbjct: 302 KEGPFCRILTEKIEDLVSGLLLPLYFAASGLKTDVTTIRGAQSWGLLVLVILTTCFGKIV 361

Query: 373 GALLASIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAA 432
           G + +S+  K+P R+AV+LG ++N++G  EL +  + K  K +ND+ F +  +  L    
Sbjct: 362 GTVGSSMLCKVPFREAVTLGFLMNTKGLVELIVLNIGKDRKVLNDQAFAILVLMALFTTF 421

Query: 433 VIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQEDVPSAINLLDALNPT-R 492
           +  PI+  ++ P+R+   YK RT+     + +L +L C H   ++P+ INL+++   T +
Sbjct: 422 ITTPIVMLIYKPARKGAPYKHRTIQRKDHDSELRILACFHSTRNIPTLINLIESSRGTGK 481

Query: 493 RSHLYVYMLHLVELLGRATPQLIHHKLTK----VRTSRHSPSEPIVNAFKYFGQRNREIV 552
           +  L VY +HL+EL  R++   + HK       +       ++ +V AF+ +  ++   V
Sbjct: 482 KGRLCVYAMHLMELSERSSAIAMVHKARNNGLPIWNKIERSTDQMVIAFEAY--QHLRAV 541

Query: 553 KIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKNKMKMVNLHILD 612
            + P TAIS  +S+H+D+C+ AH+K+ ++IL+PFHK    +G M    ++   VN  +L 
Sbjct: 542 AVRPMTAISGLSSIHEDICTSAHQKRVAMILLPFHKHQRMDGAMESIGHRFHEVNQRVLQ 601

Query: 613 KAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINLTM 672
           +APCSV ++V+RG+   S+ +A+ +  ++VV+ F GG DDREA+  G +MVEHP I LT+
Sbjct: 602 RAPCSVGILVDRGLGGTSQVVASEV-AYKVVIPFFGGLDDREALAYGMKMVEHPGITLTV 661

Query: 673 ---------IRLLEDMDIPSDNVNERMLDNEAVFEFQQIMANNYRVRYIEEVVKDSTGTV 732
                    ++  E  +       E+  D E V E       N  + Y E VV+     +
Sbjct: 662 YKFVAARGTLKRFEKSEHDEKEKKEKETDEEFVRELMNDPRGNESLAYEERVVESKDDII 721

Query: 733 SILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEALASSDFMGNTMILVVQ 779
           + L+SM +  +L +VGR     +  V  LV   +  ELG +G  L+SS+F     +LVVQ
Sbjct: 722 ATLKSM-SKCNLFVVGR-----NAAVASLVKSTDCPELGPVGRLLSSSEFSTTASVLVVQ 777

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022983542.10.0e+0071.75cation/H(+) antiporter 15-like [Cucurbita maxima][more]
XP_023528659.10.0e+0071.25cation/H(+) antiporter 15-like [Cucurbita pepo subsp. pepo][more]
XP_022934362.10.0e+0071.38cation/H(+) antiporter 15-like [Cucurbita moschata][more]
KAG6581108.10.0e+0071.00Cation/H(+) antiporter 15, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7017840.10.0e+0069.95Cation/H(+) antiporter 15 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q9SIT54.7e-13836.47Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1[more]
Q9LMJ17.6e-11232.35Cation/H(+) antiporter 14 OS=Arabidopsis thaliana OX=3702 GN=CHX14 PE=2 SV=1[more]
O229202.7e-10932.43Cation/H(+) symporter 13 OS=Arabidopsis thaliana OX=3702 GN=CHX13 PE=1 SV=1[more]
Q9FFB88.7e-10834.37Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1[more]
Q9LUN42.9e-10330.62Cation/H(+) antiporter 19 OS=Arabidopsis thaliana OX=3702 GN=CHX19 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IZN70.0e+0071.75cation/H(+) antiporter 15-like OS=Cucurbita maxima OX=3661 GN=LOC111482115 PE=4 ... [more]
A0A6J1F2I80.0e+0071.38cation/H(+) antiporter 15-like OS=Cucurbita moschata OX=3662 GN=LOC111441553 PE=... [more]
A0A5D3DF055.3e-30268.29Cation/H(+) antiporter 15-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3BPK01.7e-30068.16cation/H(+) antiporter 15-like OS=Cucumis melo OX=3656 GN=LOC103491816 PE=4 SV=1[more]
A0A6J1EN945.0e-30067.43cation/H(+) antiporter 15-like OS=Cucurbita moschata OX=3662 GN=LOC111435106 PE=... [more]
Match NameE-valueIdentityDescription
AT2G13620.13.3e-13936.47cation/hydrogen exchanger 15 [more]
AT1G06970.15.4e-11332.35cation/hydrogen exchanger 14 [more]
AT2G30240.11.9e-11032.43Cation/hydrogen exchanger family protein [more]
AT5G22900.16.1e-10934.37cation/H+ exchanger 3 [more]
AT3G17630.12.0e-10430.62cation/H+ exchanger 19 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006153Cation/H+ exchangerPFAMPF00999Na_H_Exchangercoord: 56..431
e-value: 3.0E-30
score: 105.3
IPR038770Sodium/solute symporter superfamilyGENE3D1.20.1530.20coord: 45..441
e-value: 3.2E-51
score: 176.3
NoneNo IPR availablePANTHERPTHR32468:SF114CATION/H(+) ANTIPORTER 15-LIKE ISOFORM X1coord: 11..782
NoneNo IPR availablePANTHERPTHR32468CATION/H + ANTIPORTERcoord: 11..782

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0027469.1Lag0027469.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902600 proton transmembrane transport
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015299 solute:proton antiporter activity