Lag0027428 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0027428
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionUPF0496 protein
Locationchr8: 716255 .. 717413 (+)
RNA-Seq ExpressionLag0027428
SyntenyLag0027428
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGAATGCCTTCCTCCTCCTCCTCCGGAATCCCTAACATTCCCATCCTCCGCCGTCCACGACGACTCTGAAATTCATCTCCATCCCTCCCCGATGCAGCAATTGCAAGGTACTTTACTTTACTTTACTTTACTTTACTTTCTCCATCCAACCAATTTCCTCCCTTTCTGTGCGCTCTAACTGTTTGTGAATTTGCCTCAATGAAGATGCCTCGCCTCGATCCACCACATCCACCATCAATCTCTCTCGCATATACTACTCCAAAGTCCTCTCCAGCGACTCCTACAAGGAGATGCAGAGAATCACCAGCTCTCTCGATCTTGTGGCCGCCGCCGCCGATCAAGACCACGGCAGCAGCCACCACGATATGCTCACTCAGGTGCTTCGCCCCGTTCCAGATCACGTCCACACCGCCCTTCACCGCTCCAAATCCAATTCCCTTTCCAATCTCATATCTTCCTACTTCCAGTACAGCGAGGAAACCACCAGCCTCTGCCTCACCCTCCGGAGGAGCGTCGCTGAAGCCCGTCGACTGTATTGCCCAATCCATCATCTTCCCACCGATTCCGATTGCGACCTCGTCTTCCAAACACTCGTTAAATTCAACGAGGAGCCCAACCCTTTTCCCAGCCCTAACTCACAAACCTTCCACGAAATTCGAAACCTCCTCTGCGATCTCAAGGGCCAACTTGAGCGCCGCCTCCGCAAGTCTGGCTCCAGGATTCGCCTTGTTCGCCCTCCCGCCGCCGAGCCCAACTCCATCTGCTGCGGCGGCGCTGCCTTCGCGGCCTACATTCCTCCCAAGTTCGTCAGGAGGGAACTGGCCTATGCAGCTCAGCTTAAAGTTGCGTCTAACAATACCTTTGTTCTAAAGACTGACCTTGACACGCTTGATAGCCTCGTCGGCCGTCTGCATAACGCCGTGGAGGATGACAAGCGTTTCATTCGGCTGGGTTTGAACATAGGGAATGATGAGCACACAGTTCAGGAGGTGCTCATACAGTTAAGGAAGAACCACCAGAATTTGGGGCCTTACCTTGATCTTCTTGAGCAAAAGATATGCACTTGTTTTACCACCGTCAACCGCTCAAGATCTAAACTTCTCGAGCAGATTCTACTTCATCAAAATAGTTCTGAGTCTTCTGATCCCCAATAG

mRNA sequence

ATGTTGGAATGCCTTCCTCCTCCTCCTCCGGAATCCCTAACATTCCCATCCTCCGCCGTCCACGACGACTCTGAAATTCATCTCCATCCCTCCCCGATGCAGCAATTGCAAGATGCCTCGCCTCGATCCACCACATCCACCATCAATCTCTCTCGCATATACTACTCCAAAGTCCTCTCCAGCGACTCCTACAAGGAGATGCAGAGAATCACCAGCTCTCTCGATCTTGTGGCCGCCGCCGCCGATCAAGACCACGGCAGCAGCCACCACGATATGCTCACTCAGGTGCTTCGCCCCGTTCCAGATCACGTCCACACCGCCCTTCACCGCTCCAAATCCAATTCCCTTTCCAATCTCATATCTTCCTACTTCCAGTACAGCGAGGAAACCACCAGCCTCTGCCTCACCCTCCGGAGGAGCGTCGCTGAAGCCCGTCGACTGTATTGCCCAATCCATCATCTTCCCACCGATTCCGATTGCGACCTCGTCTTCCAAACACTCGTTAAATTCAACGAGGAGCCCAACCCTTTTCCCAGCCCTAACTCACAAACCTTCCACGAAATTCGAAACCTCCTCTGCGATCTCAAGGGCCAACTTGAGCGCCGCCTCCGCAAGTCTGGCTCCAGGATTCGCCTTGTTCGCCCTCCCGCCGCCGAGCCCAACTCCATCTGCTGCGGCGGCGCTGCCTTCGCGGCCTACATTCCTCCCAAGTTCGTCAGGAGGGAACTGGCCTATGCAGCTCAGCTTAAAGTTGCGTCTAACAATACCTTTGTTCTAAAGACTGACCTTGACACGCTTGATAGCCTCGTCGGCCGTCTGCATAACGCCGTGGAGGATGACAAGCGTTTCATTCGGCTGGGTTTGAACATAGGGAATGATGAGCACACAGTTCAGGAGGTGCTCATACAGTTAAGGAAGAACCACCAGAATTTGGGGCCTTACCTTGATCTTCTTGAGCAAAAGATATGCACTTGTTTTACCACCGTCAACCGCTCAAGATCTAAACTTCTCGAGCAGATTCTACTTCATCAAAATAGTTCTGAGTCTTCTGATCCCCAATAG

Coding sequence (CDS)

ATGTTGGAATGCCTTCCTCCTCCTCCTCCGGAATCCCTAACATTCCCATCCTCCGCCGTCCACGACGACTCTGAAATTCATCTCCATCCCTCCCCGATGCAGCAATTGCAAGATGCCTCGCCTCGATCCACCACATCCACCATCAATCTCTCTCGCATATACTACTCCAAAGTCCTCTCCAGCGACTCCTACAAGGAGATGCAGAGAATCACCAGCTCTCTCGATCTTGTGGCCGCCGCCGCCGATCAAGACCACGGCAGCAGCCACCACGATATGCTCACTCAGGTGCTTCGCCCCGTTCCAGATCACGTCCACACCGCCCTTCACCGCTCCAAATCCAATTCCCTTTCCAATCTCATATCTTCCTACTTCCAGTACAGCGAGGAAACCACCAGCCTCTGCCTCACCCTCCGGAGGAGCGTCGCTGAAGCCCGTCGACTGTATTGCCCAATCCATCATCTTCCCACCGATTCCGATTGCGACCTCGTCTTCCAAACACTCGTTAAATTCAACGAGGAGCCCAACCCTTTTCCCAGCCCTAACTCACAAACCTTCCACGAAATTCGAAACCTCCTCTGCGATCTCAAGGGCCAACTTGAGCGCCGCCTCCGCAAGTCTGGCTCCAGGATTCGCCTTGTTCGCCCTCCCGCCGCCGAGCCCAACTCCATCTGCTGCGGCGGCGCTGCCTTCGCGGCCTACATTCCTCCCAAGTTCGTCAGGAGGGAACTGGCCTATGCAGCTCAGCTTAAAGTTGCGTCTAACAATACCTTTGTTCTAAAGACTGACCTTGACACGCTTGATAGCCTCGTCGGCCGTCTGCATAACGCCGTGGAGGATGACAAGCGTTTCATTCGGCTGGGTTTGAACATAGGGAATGATGAGCACACAGTTCAGGAGGTGCTCATACAGTTAAGGAAGAACCACCAGAATTTGGGGCCTTACCTTGATCTTCTTGAGCAAAAGATATGCACTTGTTTTACCACCGTCAACCGCTCAAGATCTAAACTTCTCGAGCAGATTCTACTTCATCAAAATAGTTCTGAGTCTTCTGATCCCCAATAG

Protein sequence

MLECLPPPPPESLTFPSSAVHDDSEIHLHPSPMQQLQDASPRSTTSTINLSRIYYSKVLSSDSYKEMQRITSSLDLVAAAADQDHGSSHHDMLTQVLRPVPDHVHTALHRSKSNSLSNLISSYFQYSEETTSLCLTLRRSVAEARRLYCPIHHLPTDSDCDLVFQTLVKFNEEPNPFPSPNSQTFHEIRNLLCDLKGQLERRLRKSGSRIRLVRPPAAEPNSICCGGAAFAAYIPPKFVRRELAYAAQLKVASNNTFVLKTDLDTLDSLVGRLHNAVEDDKRFIRLGLNIGNDEHTVQEVLIQLRKNHQNLGPYLDLLEQKICTCFTTVNRSRSKLLEQILLHQNSSESSDPQ
Homology
BLAST of Lag0027428 vs. NCBI nr
Match: XP_008449985.1 (PREDICTED: UPF0496 protein At3g19330 isoform X1 [Cucumis melo])

HSP 1 Score: 408.3 bits (1048), Expect = 6.8e-110
Identity = 236/367 (64.31%), Postives = 266/367 (72.48%), Query Frame = 0

Query: 1   MLECLPPPPPESLTFPSSAVHDDSEIHLHPSPMQ-QLQDASPRSTTSTINLSRIYYSKVL 60
           MLECLP   P   TF +          +H +PM      +SP ++T+TINLSRIY + +L
Sbjct: 1   MLECLPAGSP---TFST----------MHQAPMDASSSSSSPVTSTTTINLSRIYSTAIL 60

Query: 61  SSDSYKEMQRITSSLDLV---AAAADQDHGSSHHDMLTQVLRPVPDHVHTALHRSKSNSL 120
            ++S+KEM RITSS+DLV   AA  +QD       MLTQVL P  D V  ALHRSKS SL
Sbjct: 61  QTNSFKEMVRITSSMDLVVPDAADHEQDQLQDLSGMLTQVLSPNRDRVLNALHRSKSTSL 120

Query: 121 SNLISSYFQYSEETTSLCLTLRRSVAEARRLYCPIH----HLPTDS----DCDLVFQTLV 180
            NLISSYF YSE TT LCLTLR  VA+ARRLY PI+     LP DS     C+LV Q   
Sbjct: 121 LNLISSYFDYSERTTRLCLTLRGIVAQARRLYSPINTLLQDLPIDSLSQTHCELVVQEFH 180

Query: 181 KFNEEPNPFPSPNSQTFHEIRNLLCDLKGQLERRLRKSGSRIRLVRPPAAEPNSICC--G 240
           KF ++ NPFP P+S TFH IR+  CDL  QLERRLRKS SRIRLVR P  +PN++ C  G
Sbjct: 181 KFKDDANPFPRPDSLTFHPIRDHFCDLNRQLERRLRKSSSRIRLVRSPNPDPNTVLCCRG 240

Query: 241 GAAFAAYIPPKFVRRELAYAAQLKVASNNTFVLKTDLDTLDSLVGRLHNAVEDDKRFIRL 300
           GAA  AYIPPKF RRELAYAAQLKVAS NT+VLKTDLDTLDSLVGRLHN VEDDKRFIRL
Sbjct: 241 GAAITAYIPPKFARRELAYAAQLKVASRNTYVLKTDLDTLDSLVGRLHNTVEDDKRFIRL 300

Query: 301 GLNIGNDEHTVQEVLIQLRKNHQNLGPYLDLLEQKICTCFTTVNRSRSKLLEQILLHQNS 354
           GLN+GNDEHTVQEVLI L KNH NLG YLDLLEQKI TC  TVN SRS+LL++ILLHQ S
Sbjct: 301 GLNMGNDEHTVQEVLIHLGKNHPNLGHYLDLLEQKITTCLITVNHSRSQLLKEILLHQTS 354

BLAST of Lag0027428 vs. NCBI nr
Match: XP_022983457.1 (UPF0496 protein At3g19330 [Cucurbita maxima])

HSP 1 Score: 406.8 bits (1044), Expect = 2.0e-109
Identity = 230/361 (63.71%), Postives = 266/361 (73.68%), Query Frame = 0

Query: 1   MLECLPPPPPESLTFPSSAVHDDSEIHLHPSPMQQLQDASPRSTTSTINLSRIYYSKVLS 60
           MLECLPPP   + +     + ++      P P   +  +SP   T+TI+LS  Y + VL 
Sbjct: 1   MLECLPPPESAAFSPMQHGLIENRTAPHGPPPPPPMAASSPE--TTTISLSHAYAAAVLQ 60

Query: 61  SDSYKEMQRITSSLDLVAAAADQDHGSSHHDMLTQVLRPVPDHVHTALHRSKSNSLS--N 120
           ++S+KEM RITSS+DL+A A   DH  SH  ML QVL P  D V  AL RSKSNS S  N
Sbjct: 61  TNSFKEMVRITSSMDLLAVA---DHDQSHSGMLAQVLAPDRDCVLDALQRSKSNSTSLFN 120

Query: 121 LISSYFQYSEETTSLCLTLRRSVAEARRLYCPIH----HLPTD----SDCDLVFQTLVKF 180
           L SSYFQYSEETTSLCLTLR  VA+ARRLYCPI      +P D    S CDLVFQ  +KF
Sbjct: 121 LNSSYFQYSEETTSLCLTLRIIVAKARRLYCPIDTLLCEIPIDSLSRSHCDLVFQEFLKF 180

Query: 181 NEEPNPFPSPNSQTFHEIRNLLCDLKGQLERRLRKSGSRIRLVRPPAAEPNSICC-GGAA 240
           N EPNPFP P+S TF  IR+L  DLK QL+RRL KS SRI LVRPPAAEPNS+CC GGAA
Sbjct: 181 NAEPNPFPRPDSHTFDRIRDLFSDLKRQLDRRLSKSSSRIHLVRPPAAEPNSLCCGGGAA 240

Query: 241 FAAYIPPKFVRRELAYAAQLKVASNNTFVLKTDLDTLDSLVGRLHNAVEDDKRFIRLGLN 300
             AYIPP+FV+RELAYAAQLKVA+ NT++LKTDLDTLDSLV RLHN VEDDKR I+ GLN
Sbjct: 241 IMAYIPPRFVKRELAYAAQLKVATRNTYILKTDLDTLDSLVSRLHNNVEDDKRSIQDGLN 300

Query: 301 IGNDEHTVQEVLIQLRKNHQNLGPYLDLLEQKICTCFTTVNRSRSKLLEQILLHQNSSES 351
           +GND+H VQE+L QL +NHQ +G YLDLLEQKI TCF T+NRSRS+LLE+ILLHQ SS S
Sbjct: 301 MGNDQHIVQELLKQLGRNHQKMGHYLDLLEQKITTCFNTINRSRSQLLEKILLHQTSSGS 356

BLAST of Lag0027428 vs. NCBI nr
Match: XP_004145706.1 (UPF0496 protein At3g19330 [Cucumis sativus])

HSP 1 Score: 406.4 bits (1043), Expect = 2.6e-109
Identity = 233/366 (63.66%), Postives = 265/366 (72.40%), Query Frame = 0

Query: 1   MLECLPPPPPESLTFPSSAVHDDSEIHLHPSPMQQLQDASPRSTTSTINLSRIYYSKVLS 60
           MLECLPP  P   TF S          +H +PM     +SP ++T+TINLSRIY + +L 
Sbjct: 1   MLECLPPGSP---TFSS----------MHQAPM-DASSSSPVTSTTTINLSRIYSTAILQ 60

Query: 61  SDSYKEMQRITSSLDLVAAAA---DQDHGSSHHDMLTQVLRPVPDHVHTALHRSKSNSLS 120
           ++S+KEM RITSS+DLVAA     D+D       MLTQVL P  D V  AL RSKS SL 
Sbjct: 61  TNSFKEMVRITSSMDLVAADTADHDEDQLQDLSGMLTQVLSPNRDRVLNALQRSKSTSLL 120

Query: 121 NLISSYFQYSEETTSLCLTLRRSVAEARRLYCPIHHLPTDSD--------CDLVFQTLVK 180
           NLISSYF YSE+TT LCLTLR  VA+ARRLY PI+ L  D          C+LV Q   K
Sbjct: 121 NLISSYFDYSEKTTRLCLTLRCIVAQARRLYSPINTLLQDLPNYSLSQPYCELVVQEFHK 180

Query: 181 FNEEPNPFPSPNSQTFHEIRNLLCDLKGQLERRLRKSGSRIRLVRPPAAEPNSICC--GG 240
           F ++ NPFP P+S TFH IR+  CDLK QLERRLRKS SRIRLVR P  +PN++ C  GG
Sbjct: 181 FKDDANPFPRPDSLTFHPIRDHFCDLKRQLERRLRKSSSRIRLVRSPNPDPNTVLCCGGG 240

Query: 241 AAFAAYIPPKFVRRELAYAAQLKVASNNTFVLKTDLDTLDSLVGRLHNAVEDDKRFIRLG 300
           AA  AYIPPKF RRELAYAAQLKVAS NT+VLKTDLDTLDSLVGRLHN VEDDKRFIRLG
Sbjct: 241 AAITAYIPPKFARRELAYAAQLKVASRNTYVLKTDLDTLDSLVGRLHNTVEDDKRFIRLG 300

Query: 301 LNIGNDEHTVQEVLIQLRKNHQNLGPYLDLLEQKICTCFTTVNRSRSKLLEQILLHQNSS 354
           LN+GNDEHT+QEVLI L KNH NL  YLDLLEQK+ TC  TVN SRS+LL++ILLHQ SS
Sbjct: 301 LNMGNDEHTIQEVLIHLGKNHPNLRNYLDLLEQKLTTCLITVNHSRSQLLKEILLHQTSS 352

BLAST of Lag0027428 vs. NCBI nr
Match: KAE8650801.1 (hypothetical protein Csa_017445 [Cucumis sativus])

HSP 1 Score: 406.4 bits (1043), Expect = 2.6e-109
Identity = 233/366 (63.66%), Postives = 265/366 (72.40%), Query Frame = 0

Query: 1   MLECLPPPPPESLTFPSSAVHDDSEIHLHPSPMQQLQDASPRSTTSTINLSRIYYSKVLS 60
           MLECLPP  P   TF S          +H +PM     +SP ++T+TINLSRIY + +L 
Sbjct: 1   MLECLPPGSP---TFSS----------MHQAPM-DASSSSPVTSTTTINLSRIYSTAILQ 60

Query: 61  SDSYKEMQRITSSLDLVAAAA---DQDHGSSHHDMLTQVLRPVPDHVHTALHRSKSNSLS 120
           ++S+KEM RITSS+DLVAA     D+D       MLTQVL P  D V  AL RSKS SL 
Sbjct: 61  TNSFKEMVRITSSMDLVAADTADHDEDQLQDLSGMLTQVLSPNRDRVLNALQRSKSTSLL 120

Query: 121 NLISSYFQYSEETTSLCLTLRRSVAEARRLYCPIHHLPTDSD--------CDLVFQTLVK 180
           NLISSYF YSE+TT LCLTLR  VA+ARRLY PI+ L  D          C+LV Q   K
Sbjct: 121 NLISSYFDYSEKTTRLCLTLRCIVAQARRLYSPINTLLQDLPNYSLSQPYCELVVQEFHK 180

Query: 181 FNEEPNPFPSPNSQTFHEIRNLLCDLKGQLERRLRKSGSRIRLVRPPAAEPNSICC--GG 240
           F ++ NPFP P+S TFH IR+  CDLK QLERRLRKS SRIRLVR P  +PN++ C  GG
Sbjct: 181 FKDDANPFPRPDSLTFHPIRDHFCDLKRQLERRLRKSSSRIRLVRSPNPDPNTVLCCGGG 240

Query: 241 AAFAAYIPPKFVRRELAYAAQLKVASNNTFVLKTDLDTLDSLVGRLHNAVEDDKRFIRLG 300
           AA  AYIPPKF RRELAYAAQLKVAS NT+VLKTDLDTLDSLVGRLHN VEDDKRFIRLG
Sbjct: 241 AAITAYIPPKFARRELAYAAQLKVASRNTYVLKTDLDTLDSLVGRLHNTVEDDKRFIRLG 300

Query: 301 LNIGNDEHTVQEVLIQLRKNHQNLGPYLDLLEQKICTCFTTVNRSRSKLLEQILLHQNSS 354
           LN+GNDEHT+QEVLI L KNH NL  YLDLLEQK+ TC  TVN SRS+LL++ILLHQ SS
Sbjct: 301 LNMGNDEHTIQEVLIHLGKNHPNLRNYLDLLEQKLTTCLITVNHSRSQLLKEILLHQTSS 352

BLAST of Lag0027428 vs. NCBI nr
Match: KAG6581140.1 (UPF0496 protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017876.1 UPF0496 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 406.0 bits (1042), Expect = 3.4e-109
Identity = 231/362 (63.81%), Postives = 266/362 (73.48%), Query Frame = 0

Query: 1   MLECLPPPPPESLTFP-SSAVHDDSEIHLHPSPMQQLQDASPRSTTSTINLSRIYYSKVL 60
           MLECLPPP   +   P    + ++      P P   +  +SP   T+TI+LS  Y + VL
Sbjct: 1   MLECLPPPESAAAFSPMQHGLIENGTAPPGPPPPPPMAASSPE--TTTIDLSHAYAAAVL 60

Query: 61  SSDSYKEMQRITSSLDLVAAAADQDHGSSHHDMLTQVLRPVPDHVHTALHRSKSNSLS-- 120
            ++S+KEM RITSS+DL+A A   DHG SH  ML QVL P  D V  AL RSKS S S  
Sbjct: 61  QTNSFKEMVRITSSMDLLAVA---DHGQSHSGMLAQVLDPNRDCVLDALQRSKSTSTSLF 120

Query: 121 NLISSYFQYSEETTSLCLTLRRSVAEARRLYCPIH----HLPTD----SDCDLVFQTLVK 180
           NL S YFQYSEETTSLCLTLR  VA+ARRLYCPI      +P D    S CDLVFQ   K
Sbjct: 121 NLNSCYFQYSEETTSLCLTLRIIVAKARRLYCPIDTLLCEIPIDSLSPSHCDLVFQEFRK 180

Query: 181 FNEEPNPFPSPNSQTFHEIRNLLCDLKGQLERRLRKSGSRIRLVRPPAAEPNSICC-GGA 240
           FN EPNPFP P+S TF  IR+   DL+ QL+RRLRKS SRI LVRPPAAEPNS+CC GGA
Sbjct: 181 FNAEPNPFPRPDSHTFDGIRDHFSDLRRQLDRRLRKSSSRIHLVRPPAAEPNSLCCGGGA 240

Query: 241 AFAAYIPPKFVRRELAYAAQLKVASNNTFVLKTDLDTLDSLVGRLHNAVEDDKRFIRLGL 300
           A  AYIPP+FV+RELAYAAQLKVA+ NT++LKTDLDTLDSLV RLHN VEDDKR I+ GL
Sbjct: 241 AITAYIPPRFVKRELAYAAQLKVATRNTYILKTDLDTLDSLVSRLHNTVEDDKRSIQTGL 300

Query: 301 NIGNDEHTVQEVLIQLRKNHQNLGPYLDLLEQKICTCFTTVNRSRSKLLEQILLHQNSSE 351
           N+GND+HTVQE+L QL KNHQN+G +LDLLEQKI TCF T+NRSRS+LLE+ILLHQ SS 
Sbjct: 301 NMGNDQHTVQELLKQLGKNHQNMGNFLDLLEQKITTCFNTINRSRSQLLEKILLHQTSSG 357

BLAST of Lag0027428 vs. ExPASy Swiss-Prot
Match: Q9LT84 (UPF0496 protein At3g19330 OS=Arabidopsis thaliana OX=3702 GN=At3g19330 PE=2 SV=2)

HSP 1 Score: 181.8 bits (460), Expect = 1.3e-44
Identity = 141/388 (36.34%), Postives = 188/388 (48.45%), Query Frame = 0

Query: 1   MLECLP--PPPPESLTFPSSAVHDDSEIHLHPSPMQQLQDASPRSTTSTINLSRIYYSKV 60
           M  CL   P  PE+        H   E  +  +       +SP     T NLSR   +  
Sbjct: 1   MRNCLSFNPASPEASLGDDPLPHPSLESGVASTSRNDASISSP-----TFNLSR-ELAHA 60

Query: 61  LSSDSYKEMQRITSSLDLVAAAADQDHGSSHHD---MLTQVLRPVPDHVHTALHRSKSNS 120
             + SY +   + S + +V       H     D   +L+QVL+P  + V  A+   K  +
Sbjct: 61  FQTPSYHD---VRSRVHVVVDLTQIHHRLIQPDIELLLSQVLQPNKECVQEAIRHVKQTT 120

Query: 121 LSNLISSYFQYSEETTSLCLTLRRSVAEAR-RLYCPI---------HHLPT--DSDCDLV 180
           L+NL+S+YFQ+SE+ T LCL L ++V  AR  LY P+           LP   +S CDL 
Sbjct: 121 LTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDLFNIFPGDSLPAIDESLCDLA 180

Query: 181 FQTLVKFNEEPNPFPSPNSQTFHEIRNLLCDLKGQLERRLRKSGSRIRLVRPPAAEPNSI 240
           F   +K +   NPF SP S +F + +     LK  L+RRLRKS SR+RL+   A   +S+
Sbjct: 181 FDVFLKLDTFENPFSSPESYSFRDTQLCFSQLKHNLDRRLRKSRSRVRLIH-HATAGSSL 240

Query: 241 CCGGAAFAA-------------------------YIPPKFVRRELAYAAQLKVASNNTFV 300
           C   A  A                          Y+P  F R+EL    QL  AS  TFV
Sbjct: 241 CLVAAVVAVAASAVVIASHALPILLVVAGPLCSPYLPHSFKRKELTNICQLNAASKGTFV 300

Query: 301 LKTDLDTLDSLVGRLHNAVEDDKRFIRLGLNIGNDEHTVQEVLIQLRKNHQNLGPYLDLL 347
           L  DLDT+D LV RLH  +E DK  IRLGL  G D H++QE+L  LRK+H  L   L  L
Sbjct: 301 LNKDLDTIDRLVSRLHTGIEYDKFLIRLGLERGRDVHSIQEILKLLRKSHLPLTHQLKDL 360

BLAST of Lag0027428 vs. ExPASy Swiss-Prot
Match: Q9LJK4 (UPF0496 protein At3g19250 OS=Arabidopsis thaliana OX=3702 GN=At3g19250 PE=2 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 8.2e-42
Identity = 130/375 (34.67%), Postives = 181/375 (48.27%), Query Frame = 0

Query: 10  PESLTF-PSSAVHDDSEIHL-HPSPMQQLQDASPRSTTSTINLSRIYYSKVLSSDSYKEM 69
           P   TF P+S      + HL HPSP          S  ST NLS    +    + SY ++
Sbjct: 2   PHCFTFKPASPEGSLGDDHLPHPSP--------EGSVASTFNLSH-ELAHAFQTPSYHDI 61

Query: 70  QRITSSLDLVAAAADQDHGSSHHDMLTQVLRPVPDHVHTA--LHRSKSNSLSNLISSYFQ 129
           +     +D      +          L+Q LRP  + V  A  L  +K  +L+NL+S+YFQ
Sbjct: 62  RSRLLVIDPTQENLEL--------FLSQELRPNNESVQEALSLRHAKQTTLTNLVSTYFQ 121

Query: 130 YSEETTSLCLTLRRSVAEAR-RLYCPI-----------HHLPTDSDCDLVFQTLVKFNEE 189
           +SE+ T  CL L ++V  AR  LY P+           H    +S C+L F   +K +  
Sbjct: 122 HSEDATRFCLNLYQNVHSARCHLYTPLLDLFNIFPRDSHSAIDESFCNLAFDVFLKLDTF 181

Query: 190 PNPFPSPNSQTFHEIRNLLCDLKGQLERRLRKSGSRIRLVRPPAAE-------------- 249
            NPF SP S +F + +     L  +L+ R+RKS SR+RL+    A               
Sbjct: 182 ENPFASPESHSFQDTQLCFYQLADKLDTRIRKSKSRVRLLHHATAGSALCLVTAVVVVAA 241

Query: 250 ----------PNSICCGGAAFAAYIPPKFVRRELAYAAQLKVASNNTFVLKTDLDTLDSL 309
                     P  +   G     Y+P  F ++EL+   QL VA+  TF L  DLDT+D L
Sbjct: 242 SAAFIAYHALPTILVVAGPLCTPYLPHSFKKKELSNIFQLNVAAKGTFALNKDLDTIDRL 301

Query: 310 VGRLHNAVEDDKRFIRLGLNIGNDEHTVQEVLIQLRKNHQNLGPYLDLLEQKICTCFTTV 345
           V RLH  V++DK  IRLGL  G D +T+ E + QLRK+H N    L++L   IC  FT V
Sbjct: 302 VSRLHTGVKNDKLLIRLGLERGRDVYTIPEFVKQLRKSHVNHTHQLEVLVDHICRWFTNV 359

BLAST of Lag0027428 vs. ExPASy Swiss-Prot
Match: A2YH25 (Putative UPF0496 protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_023618 PE=3 SV=2)

HSP 1 Score: 72.8 bits (177), Expect = 8.8e-12
Identity = 70/300 (23.33%), Postives = 115/300 (38.33%), Query Frame = 0

Query: 79  AAADQDHGSSHHDMLTQVLRPVPDHV-------HTALHRSKSNSLSNLISSYFQYSEETT 138
           AA D +   S+  +   VL P P+ +            R + + +  L+  YF  +EE  
Sbjct: 79  AADDSEQSCSYTVLDDFVLEPSPESLARGARLQQRRRRRPRRHRVETLLIEYFDVTEEAC 138

Query: 139 SLCLTLRRSVAEARRLYCPIHHL---PTDSDCDLVFQTLVKFNEEPNPFPSPNSQTFHEI 198
             C  L  ++  ARR +  +  L       D D     L +     NP    +   FH++
Sbjct: 139 EACSALLAAIGAARRHHLTLRRLLLRLDGGDDDDAKDALARHVRLDNPLSPGSLSEFHDV 198

Query: 199 RNLLCDLKGQL---ERRLRKSGSRIRLVRPPAAEPNSICCGGAAFAAYI----------- 258
                 L  +L   +RRLR+    +R+ R  AA      C  A  AA +           
Sbjct: 199 HARCSPLASRLAAAQRRLRRLARALRIARGTAAAALVGACAAAIVAAVVLAAHALVGIGV 258

Query: 259 --------------------PPKFVRRELAYA-AQLKVASNNTFVLKTDLDTLDSLVGRL 318
                                 K   R  A A A L  A+   +++  DLDT+  +V R 
Sbjct: 259 AAAAFGATPAGAARWWARRAAEKVSSRHYARAGATLDAAARGAYIVGRDLDTVSRMVRRA 318

Query: 319 HNAVEDDKRFIRLGLNIGNDEHTVQEVLIQLRKNHQNLGPYLDLLEQKICTCFTTVNRSR 334
           H+ +E  +   R+ +    +   +QEV  +  +  ++L   L  LE+ +C C  T+NR+R
Sbjct: 319 HDELEHGRDVARIAMRGHGERPLLQEVAREEEECEEDLRAQLAELEEHVCLCLITINRTR 378

BLAST of Lag0027428 vs. ExPASy Swiss-Prot
Match: Q5Z8N6 (Putative UPF0496 protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0718300 PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 8.8e-12
Identity = 70/300 (23.33%), Postives = 115/300 (38.33%), Query Frame = 0

Query: 79  AAADQDHGSSHHDMLTQVLRPVPDHV-------HTALHRSKSNSLSNLISSYFQYSEETT 138
           AA D +   S+  +   VL P P+ +            R + + +  L+  YF  +EE  
Sbjct: 79  AADDSEQSCSYTVLDDFVLEPSPESLARGARLQQRRRRRPRRHRVETLLIEYFDVTEEAC 138

Query: 139 SLCLTLRRSVAEARRLYCPIHHL---PTDSDCDLVFQTLVKFNEEPNPFPSPNSQTFHEI 198
             C  L  ++  ARR +  +  L       D D     L +     NP    +   FH++
Sbjct: 139 EACSALLAAIGAARRHHLTLRRLLLRLDGGDDDDAKDALARHVRLDNPLSPGSLSEFHDV 198

Query: 199 RNLLCDLKGQL---ERRLRKSGSRIRLVRPPAAEPNSICCGGAAFAAYI----------- 258
                 L  +L   +RRLR+    +R+ R  AA      C  A  AA +           
Sbjct: 199 HARCSPLASRLAAAQRRLRRLARALRIARGTAAAALVGACAAAIVAAVVLAAHALVGIGV 258

Query: 259 --------------------PPKFVRRELAYA-AQLKVASNNTFVLKTDLDTLDSLVGRL 318
                                 K   R  A A A L  A+   +++  DLDT+  +V R 
Sbjct: 259 AAAAFGATPAGAARWWGRRAAEKVSSRHYARAGATLDAAARGAYIVGRDLDTVSRMVRRA 318

Query: 319 HNAVEDDKRFIRLGLNIGNDEHTVQEVLIQLRKNHQNLGPYLDLLEQKICTCFTTVNRSR 334
           H+ +E  +   R+ +    +   +QEV  +  +  ++L   L  LE+ +C C  T+NR+R
Sbjct: 319 HDELEHGRDVARIAMRGHGERPLLQEVAREEEECEEDLRAQLAELEEHVCLCLITINRTR 378

BLAST of Lag0027428 vs. ExPASy Swiss-Prot
Match: Q9SMU4 (UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.1e-09
Identity = 67/259 (25.87%), Postives = 117/259 (45.17%), Query Frame = 0

Query: 119 LISSYFQYSEETTSLCLTLRRSVAEARRLY---CPIHHLPTDSDCDLVFQTLVKFNEEPN 178
           L+S YF  +     LC  L +++   R  Y    P  H    +   L+ Q   + ++  +
Sbjct: 153 LLSDYFLETANAFLLCTQLLKNIHHLRSKYESLKPKFHSENHNSLALIDQ-FTEISKWFD 212

Query: 179 PFPSPNSQTFHEIRNLLCDLKGQLERRLRKSGSRIRLVRPPAAEPN----------SICC 238
           PF S  S+    IR+    L  +LE R  K+ ++++L+                   +  
Sbjct: 213 PFISSGSR-IQLIRSGCLYLLKRLESRRDKTRAKLKLINGLTHSSGLLVLALTTTLIVTI 272

Query: 239 GGAAFAAYI----------PPKFVRRELA-YAAQLKVASNNTFVLKTDLDTLDSLVGRLH 298
              AFA ++           P  +R +L   AA+L VA+  T++L  DLDT+  LV R++
Sbjct: 273 ASHAFALFLAAPTLLASQFKPAGLRNKLTKTAARLDVAAKGTYILSRDLDTISRLVTRIN 332

Query: 299 NAVEDDKRFIRLGLNIGNDE-HTVQEVLIQLRKNHQNLGPYLDLLEQKICTCFTTVNRSR 353
           + V   +      +  G+      +EV  +L++  ++    LD LE+ I  CF T+NR+R
Sbjct: 333 DEVNHVRAMAEFWVGRGSGRVRGSEEVARELKRCEESFSEELDELEEHIYLCFMTINRAR 392

BLAST of Lag0027428 vs. ExPASy TrEMBL
Match: A0A1S3BP81 (UPF0496 protein At3g19330 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491707 PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 3.3e-110
Identity = 236/367 (64.31%), Postives = 266/367 (72.48%), Query Frame = 0

Query: 1   MLECLPPPPPESLTFPSSAVHDDSEIHLHPSPMQ-QLQDASPRSTTSTINLSRIYYSKVL 60
           MLECLP   P   TF +          +H +PM      +SP ++T+TINLSRIY + +L
Sbjct: 1   MLECLPAGSP---TFST----------MHQAPMDASSSSSSPVTSTTTINLSRIYSTAIL 60

Query: 61  SSDSYKEMQRITSSLDLV---AAAADQDHGSSHHDMLTQVLRPVPDHVHTALHRSKSNSL 120
            ++S+KEM RITSS+DLV   AA  +QD       MLTQVL P  D V  ALHRSKS SL
Sbjct: 61  QTNSFKEMVRITSSMDLVVPDAADHEQDQLQDLSGMLTQVLSPNRDRVLNALHRSKSTSL 120

Query: 121 SNLISSYFQYSEETTSLCLTLRRSVAEARRLYCPIH----HLPTDS----DCDLVFQTLV 180
            NLISSYF YSE TT LCLTLR  VA+ARRLY PI+     LP DS     C+LV Q   
Sbjct: 121 LNLISSYFDYSERTTRLCLTLRGIVAQARRLYSPINTLLQDLPIDSLSQTHCELVVQEFH 180

Query: 181 KFNEEPNPFPSPNSQTFHEIRNLLCDLKGQLERRLRKSGSRIRLVRPPAAEPNSICC--G 240
           KF ++ NPFP P+S TFH IR+  CDL  QLERRLRKS SRIRLVR P  +PN++ C  G
Sbjct: 181 KFKDDANPFPRPDSLTFHPIRDHFCDLNRQLERRLRKSSSRIRLVRSPNPDPNTVLCCRG 240

Query: 241 GAAFAAYIPPKFVRRELAYAAQLKVASNNTFVLKTDLDTLDSLVGRLHNAVEDDKRFIRL 300
           GAA  AYIPPKF RRELAYAAQLKVAS NT+VLKTDLDTLDSLVGRLHN VEDDKRFIRL
Sbjct: 241 GAAITAYIPPKFARRELAYAAQLKVASRNTYVLKTDLDTLDSLVGRLHNTVEDDKRFIRL 300

Query: 301 GLNIGNDEHTVQEVLIQLRKNHQNLGPYLDLLEQKICTCFTTVNRSRSKLLEQILLHQNS 354
           GLN+GNDEHTVQEVLI L KNH NLG YLDLLEQKI TC  TVN SRS+LL++ILLHQ S
Sbjct: 301 GLNMGNDEHTVQEVLIHLGKNHPNLGHYLDLLEQKITTCLITVNHSRSQLLKEILLHQTS 354

BLAST of Lag0027428 vs. ExPASy TrEMBL
Match: A0A6J1IZC9 (UPF0496 protein At3g19330 OS=Cucurbita maxima OX=3661 GN=LOC111482055 PE=3 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 9.5e-110
Identity = 230/361 (63.71%), Postives = 266/361 (73.68%), Query Frame = 0

Query: 1   MLECLPPPPPESLTFPSSAVHDDSEIHLHPSPMQQLQDASPRSTTSTINLSRIYYSKVLS 60
           MLECLPPP   + +     + ++      P P   +  +SP   T+TI+LS  Y + VL 
Sbjct: 1   MLECLPPPESAAFSPMQHGLIENRTAPHGPPPPPPMAASSPE--TTTISLSHAYAAAVLQ 60

Query: 61  SDSYKEMQRITSSLDLVAAAADQDHGSSHHDMLTQVLRPVPDHVHTALHRSKSNSLS--N 120
           ++S+KEM RITSS+DL+A A   DH  SH  ML QVL P  D V  AL RSKSNS S  N
Sbjct: 61  TNSFKEMVRITSSMDLLAVA---DHDQSHSGMLAQVLAPDRDCVLDALQRSKSNSTSLFN 120

Query: 121 LISSYFQYSEETTSLCLTLRRSVAEARRLYCPIH----HLPTD----SDCDLVFQTLVKF 180
           L SSYFQYSEETTSLCLTLR  VA+ARRLYCPI      +P D    S CDLVFQ  +KF
Sbjct: 121 LNSSYFQYSEETTSLCLTLRIIVAKARRLYCPIDTLLCEIPIDSLSRSHCDLVFQEFLKF 180

Query: 181 NEEPNPFPSPNSQTFHEIRNLLCDLKGQLERRLRKSGSRIRLVRPPAAEPNSICC-GGAA 240
           N EPNPFP P+S TF  IR+L  DLK QL+RRL KS SRI LVRPPAAEPNS+CC GGAA
Sbjct: 181 NAEPNPFPRPDSHTFDRIRDLFSDLKRQLDRRLSKSSSRIHLVRPPAAEPNSLCCGGGAA 240

Query: 241 FAAYIPPKFVRRELAYAAQLKVASNNTFVLKTDLDTLDSLVGRLHNAVEDDKRFIRLGLN 300
             AYIPP+FV+RELAYAAQLKVA+ NT++LKTDLDTLDSLV RLHN VEDDKR I+ GLN
Sbjct: 241 IMAYIPPRFVKRELAYAAQLKVATRNTYILKTDLDTLDSLVSRLHNNVEDDKRSIQDGLN 300

Query: 301 IGNDEHTVQEVLIQLRKNHQNLGPYLDLLEQKICTCFTTVNRSRSKLLEQILLHQNSSES 351
           +GND+H VQE+L QL +NHQ +G YLDLLEQKI TCF T+NRSRS+LLE+ILLHQ SS S
Sbjct: 301 MGNDQHIVQELLKQLGRNHQKMGHYLDLLEQKITTCFNTINRSRSQLLEKILLHQTSSGS 356

BLAST of Lag0027428 vs. ExPASy TrEMBL
Match: A0A0A0L8I4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G600010 PE=4 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 1.2e-109
Identity = 233/366 (63.66%), Postives = 265/366 (72.40%), Query Frame = 0

Query: 1   MLECLPPPPPESLTFPSSAVHDDSEIHLHPSPMQQLQDASPRSTTSTINLSRIYYSKVLS 60
           MLECLPP  P   TF S          +H +PM     +SP ++T+TINLSRIY + +L 
Sbjct: 1   MLECLPPGSP---TFSS----------MHQAPM-DASSSSPVTSTTTINLSRIYSTAILQ 60

Query: 61  SDSYKEMQRITSSLDLVAAAA---DQDHGSSHHDMLTQVLRPVPDHVHTALHRSKSNSLS 120
           ++S+KEM RITSS+DLVAA     D+D       MLTQVL P  D V  AL RSKS SL 
Sbjct: 61  TNSFKEMVRITSSMDLVAADTADHDEDQLQDLSGMLTQVLSPNRDRVLNALQRSKSTSLL 120

Query: 121 NLISSYFQYSEETTSLCLTLRRSVAEARRLYCPIHHLPTDSD--------CDLVFQTLVK 180
           NLISSYF YSE+TT LCLTLR  VA+ARRLY PI+ L  D          C+LV Q   K
Sbjct: 121 NLISSYFDYSEKTTRLCLTLRCIVAQARRLYSPINTLLQDLPNYSLSQPYCELVVQEFHK 180

Query: 181 FNEEPNPFPSPNSQTFHEIRNLLCDLKGQLERRLRKSGSRIRLVRPPAAEPNSICC--GG 240
           F ++ NPFP P+S TFH IR+  CDLK QLERRLRKS SRIRLVR P  +PN++ C  GG
Sbjct: 181 FKDDANPFPRPDSLTFHPIRDHFCDLKRQLERRLRKSSSRIRLVRSPNPDPNTVLCCGGG 240

Query: 241 AAFAAYIPPKFVRRELAYAAQLKVASNNTFVLKTDLDTLDSLVGRLHNAVEDDKRFIRLG 300
           AA  AYIPPKF RRELAYAAQLKVAS NT+VLKTDLDTLDSLVGRLHN VEDDKRFIRLG
Sbjct: 241 AAITAYIPPKFARRELAYAAQLKVASRNTYVLKTDLDTLDSLVGRLHNTVEDDKRFIRLG 300

Query: 301 LNIGNDEHTVQEVLIQLRKNHQNLGPYLDLLEQKICTCFTTVNRSRSKLLEQILLHQNSS 354
           LN+GNDEHT+QEVLI L KNH NL  YLDLLEQK+ TC  TVN SRS+LL++ILLHQ SS
Sbjct: 301 LNMGNDEHTIQEVLIHLGKNHPNLRNYLDLLEQKLTTCLITVNHSRSQLLKEILLHQTSS 352

BLAST of Lag0027428 vs. ExPASy TrEMBL
Match: A0A6J1F7D7 (UPF0496 protein At3g19330-like OS=Cucurbita moschata OX=3662 GN=LOC111441533 PE=3 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 3.6e-109
Identity = 229/361 (63.43%), Postives = 265/361 (73.41%), Query Frame = 0

Query: 1   MLECLPPPPPESLTFPSSAVHDDSEIHLHPSPMQQLQDASPRSTTSTINLSRIYYSKVLS 60
           MLECLPPP   + +     + ++      P P   +  +SP   T+TI+LS  Y + VL 
Sbjct: 1   MLECLPPPESAAFSPVQHGLIENRTAPPGPPPPPPMAASSPE--TTTIDLSHAYAAAVLQ 60

Query: 61  SDSYKEMQRITSSLDLVAAAADQDHGSSHHDMLTQVLRPVPDHVHTALHRSKSNSLS--N 120
           ++S+KEM RITSS+DL+A A   DH  SH  ML QVL P  D V  AL RSKS S S  N
Sbjct: 61  TNSFKEMVRITSSMDLLAVA---DHDQSHSGMLAQVLDPNRDCVLDALQRSKSTSTSLFN 120

Query: 121 LISSYFQYSEETTSLCLTLRRSVAEARRLYCPIH----HLPTD----SDCDLVFQTLVKF 180
           L S YFQYSEETTSLCLTLR  VA+ARRLYCPI      +P D    S CDLVFQ   KF
Sbjct: 121 LNSCYFQYSEETTSLCLTLRIVVAKARRLYCPIDTLLCEIPIDSLSPSHCDLVFQEFRKF 180

Query: 181 NEEPNPFPSPNSQTFHEIRNLLCDLKGQLERRLRKSGSRIRLVRPPAAEPNSICC-GGAA 240
           N EPNPFP P+S TF  IR+   DL+ QL+RRLRKS SRI LVRPPAAEPNS+CC GGAA
Sbjct: 181 NAEPNPFPRPDSHTFDGIRDHFSDLRRQLDRRLRKSSSRIHLVRPPAAEPNSLCCGGGAA 240

Query: 241 FAAYIPPKFVRRELAYAAQLKVASNNTFVLKTDLDTLDSLVGRLHNAVEDDKRFIRLGLN 300
             AYIPP+FV+RELAYAAQLKVA+ NT++LKTDLDTLDSLV RLHN VEDDKR I+ GLN
Sbjct: 241 ITAYIPPRFVKRELAYAAQLKVATRNTYILKTDLDTLDSLVSRLHNTVEDDKRSIQTGLN 300

Query: 301 IGNDEHTVQEVLIQLRKNHQNLGPYLDLLEQKICTCFTTVNRSRSKLLEQILLHQNSSES 351
           +GND+HTVQE+L QL KNHQN+G +LDLLEQKI TCF T+NRSRS+LLE+ILLHQ SS S
Sbjct: 301 MGNDQHTVQELLKQLGKNHQNMGNFLDLLEQKITTCFNTINRSRSQLLEKILLHQTSSGS 356

BLAST of Lag0027428 vs. ExPASy TrEMBL
Match: A0A1S3BMN5 (UPF0496 protein At3g19330 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491707 PE=3 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 1.4e-108
Identity = 224/328 (68.29%), Postives = 251/328 (76.52%), Query Frame = 0

Query: 39  ASPRSTTSTINLSRIYYSKVLSSDSYKEMQRITSSLDLV---AAAADQDHGSSHHDMLTQ 98
           +SP ++T+TINLSRIY + +L ++S+KEM RITSS+DLV   AA  +QD       MLTQ
Sbjct: 8   SSPVTSTTTINLSRIYSTAILQTNSFKEMVRITSSMDLVVPDAADHEQDQLQDLSGMLTQ 67

Query: 99  VLRPVPDHVHTALHRSKSNSLSNLISSYFQYSEETTSLCLTLRRSVAEARRLYCPIH--- 158
           VL P  D V  ALHRSKS SL NLISSYF YSE TT LCLTLR  VA+ARRLY PI+   
Sbjct: 68  VLSPNRDRVLNALHRSKSTSLLNLISSYFDYSERTTRLCLTLRGIVAQARRLYSPINTLL 127

Query: 159 -HLPTDS----DCDLVFQTLVKFNEEPNPFPSPNSQTFHEIRNLLCDLKGQLERRLRKSG 218
             LP DS     C+LV Q   KF ++ NPFP P+S TFH IR+  CDL  QLERRLRKS 
Sbjct: 128 QDLPIDSLSQTHCELVVQEFHKFKDDANPFPRPDSLTFHPIRDHFCDLNRQLERRLRKSS 187

Query: 219 SRIRLVRPPAAEPNSICC--GGAAFAAYIPPKFVRRELAYAAQLKVASNNTFVLKTDLDT 278
           SRIRLVR P  +PN++ C  GGAA  AYIPPKF RRELAYAAQLKVAS NT+VLKTDLDT
Sbjct: 188 SRIRLVRSPNPDPNTVLCCRGGAAITAYIPPKFARRELAYAAQLKVASRNTYVLKTDLDT 247

Query: 279 LDSLVGRLHNAVEDDKRFIRLGLNIGNDEHTVQEVLIQLRKNHQNLGPYLDLLEQKICTC 338
           LDSLVGRLHN VEDDKRFIRLGLN+GNDEHTVQEVLI L KNH NLG YLDLLEQKI TC
Sbjct: 248 LDSLVGRLHNTVEDDKRFIRLGLNMGNDEHTVQEVLIHLGKNHPNLGHYLDLLEQKITTC 307

Query: 339 FTTVNRSRSKLLEQILLHQNSSESSDPQ 354
             TVN SRS+LL++ILLHQ SS+SS PQ
Sbjct: 308 LITVNHSRSQLLKEILLHQTSSDSSHPQ 335

BLAST of Lag0027428 vs. TAIR 10
Match: AT3G19330.3 (Protein of unknown function (DUF677) )

HSP 1 Score: 188.3 bits (477), Expect = 1.0e-47
Identity = 138/359 (38.44%), Postives = 184/359 (51.25%), Query Frame = 0

Query: 1   MLECLP--PPPPESLTFPSSAVHDDSEIHLHPSPMQQLQDASPRSTTSTINLSRIYYSKV 60
           M  CL   P  PE+        H   E  +  +       +SP     T NLSR   +  
Sbjct: 1   MRNCLSFNPASPEASLGDDPLPHPSLESGVASTSRNDASISSP-----TFNLSR-ELAHA 60

Query: 61  LSSDSYKEMQRITSSLDLVAAAADQDHGSSHHD---MLTQVLRPVPDHVHTALHRSKSNS 120
             + SY +   + S + +V       H     D   +L+QVL+P  + V  A+   K  +
Sbjct: 61  FQTPSYHD---VRSRVHVVVDLTQIHHRLIQPDIELLLSQVLQPNKECVQEAIRHVKQTT 120

Query: 121 LSNLISSYFQYSEETTSLCLTLRRSVAEAR-RLYCPI---------HHLPT--DSDCDLV 180
           L+NL+S+YFQ+SE+ T LCL L ++V  AR  LY P+           LP   +S CDL 
Sbjct: 121 LTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDLFNIFPGDSLPAIDESLCDLA 180

Query: 181 FQTLVKFNEEPNPFPSPNSQTFHEIRNLLCDLKGQLERRLRKSGSRIRLVRPPAAEPNSI 240
           F   +K +   NPF SP S +F + +     LK  L+RRLRKS SR+RL+    A P  +
Sbjct: 181 FDVFLKLDTFENPFSSPESYSFRDTQLCFSQLKHNLDRRLRKSRSRVRLIHHATAGP--L 240

Query: 241 CCGGAAFAAYIPPKFVRRELAYAAQLKVASNNTFVLKTDLDTLDSLVGRLHNAVEDDKRF 300
           C      + Y+P  F R+EL    QL  AS  TFVL  DLDT+D LV RLH  +E DK  
Sbjct: 241 C------SPYLPHSFKRKELTNICQLNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDKFL 300

Query: 301 IRLGLNIGNDEHTVQEVLIQLRKNHQNLGPYLDLLEQKICTCFTTVNRSRSKLLEQILL 343
           IRLGL  G D H++QE+L  LRK+H  L   L  LE  IC  FT VN++RS LL +I L
Sbjct: 301 IRLGLERGRDVHSIQEILKLLRKSHLPLTHQLKDLEDHICLWFTNVNKARSLLLTEIHL 342

BLAST of Lag0027428 vs. TAIR 10
Match: AT3G19330.2 (Protein of unknown function (DUF677) )

HSP 1 Score: 188.3 bits (477), Expect = 1.0e-47
Identity = 138/359 (38.44%), Postives = 184/359 (51.25%), Query Frame = 0

Query: 1   MLECLP--PPPPESLTFPSSAVHDDSEIHLHPSPMQQLQDASPRSTTSTINLSRIYYSKV 60
           M  CL   P  PE+        H   E  +  +       +SP     T NLSR   +  
Sbjct: 1   MRNCLSFNPASPEASLGDDPLPHPSLESGVASTSRNDASISSP-----TFNLSR-ELAHA 60

Query: 61  LSSDSYKEMQRITSSLDLVAAAADQDHGSSHHD---MLTQVLRPVPDHVHTALHRSKSNS 120
             + SY +   + S + +V       H     D   +L+QVL+P  + V  A+   K  +
Sbjct: 61  FQTPSYHD---VRSRVHVVVDLTQIHHRLIQPDIELLLSQVLQPNKECVQEAIRHVKQTT 120

Query: 121 LSNLISSYFQYSEETTSLCLTLRRSVAEAR-RLYCPI---------HHLPT--DSDCDLV 180
           L+NL+S+YFQ+SE+ T LCL L ++V  AR  LY P+           LP   +S CDL 
Sbjct: 121 LTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDLFNIFPGDSLPAIDESLCDLA 180

Query: 181 FQTLVKFNEEPNPFPSPNSQTFHEIRNLLCDLKGQLERRLRKSGSRIRLVRPPAAEPNSI 240
           F   +K +   NPF SP S +F + +     LK  L+RRLRKS SR+RL+    A P  +
Sbjct: 181 FDVFLKLDTFENPFSSPESYSFRDTQLCFSQLKHNLDRRLRKSRSRVRLIHHATAGP--L 240

Query: 241 CCGGAAFAAYIPPKFVRRELAYAAQLKVASNNTFVLKTDLDTLDSLVGRLHNAVEDDKRF 300
           C      + Y+P  F R+EL    QL  AS  TFVL  DLDT+D LV RLH  +E DK  
Sbjct: 241 C------SPYLPHSFKRKELTNICQLNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDKFL 300

Query: 301 IRLGLNIGNDEHTVQEVLIQLRKNHQNLGPYLDLLEQKICTCFTTVNRSRSKLLEQILL 343
           IRLGL  G D H++QE+L  LRK+H  L   L  LE  IC  FT VN++RS LL +I L
Sbjct: 301 IRLGLERGRDVHSIQEILKLLRKSHLPLTHQLKDLEDHICLWFTNVNKARSLLLTEIHL 342

BLAST of Lag0027428 vs. TAIR 10
Match: AT3G19330.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 181.8 bits (460), Expect = 9.6e-46
Identity = 141/388 (36.34%), Postives = 188/388 (48.45%), Query Frame = 0

Query: 1   MLECLP--PPPPESLTFPSSAVHDDSEIHLHPSPMQQLQDASPRSTTSTINLSRIYYSKV 60
           M  CL   P  PE+        H   E  +  +       +SP     T NLSR   +  
Sbjct: 1   MRNCLSFNPASPEASLGDDPLPHPSLESGVASTSRNDASISSP-----TFNLSR-ELAHA 60

Query: 61  LSSDSYKEMQRITSSLDLVAAAADQDHGSSHHD---MLTQVLRPVPDHVHTALHRSKSNS 120
             + SY +   + S + +V       H     D   +L+QVL+P  + V  A+   K  +
Sbjct: 61  FQTPSYHD---VRSRVHVVVDLTQIHHRLIQPDIELLLSQVLQPNKECVQEAIRHVKQTT 120

Query: 121 LSNLISSYFQYSEETTSLCLTLRRSVAEAR-RLYCPI---------HHLPT--DSDCDLV 180
           L+NL+S+YFQ+SE+ T LCL L ++V  AR  LY P+           LP   +S CDL 
Sbjct: 121 LTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDLFNIFPGDSLPAIDESLCDLA 180

Query: 181 FQTLVKFNEEPNPFPSPNSQTFHEIRNLLCDLKGQLERRLRKSGSRIRLVRPPAAEPNSI 240
           F   +K +   NPF SP S +F + +     LK  L+RRLRKS SR+RL+   A   +S+
Sbjct: 181 FDVFLKLDTFENPFSSPESYSFRDTQLCFSQLKHNLDRRLRKSRSRVRLIH-HATAGSSL 240

Query: 241 CCGGAAFAA-------------------------YIPPKFVRRELAYAAQLKVASNNTFV 300
           C   A  A                          Y+P  F R+EL    QL  AS  TFV
Sbjct: 241 CLVAAVVAVAASAVVIASHALPILLVVAGPLCSPYLPHSFKRKELTNICQLNAASKGTFV 300

Query: 301 LKTDLDTLDSLVGRLHNAVEDDKRFIRLGLNIGNDEHTVQEVLIQLRKNHQNLGPYLDLL 347
           L  DLDT+D LV RLH  +E DK  IRLGL  G D H++QE+L  LRK+H  L   L  L
Sbjct: 301 LNKDLDTIDRLVSRLHTGIEYDKFLIRLGLERGRDVHSIQEILKLLRKSHLPLTHQLKDL 360

BLAST of Lag0027428 vs. TAIR 10
Match: AT3G19250.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 172.6 bits (436), Expect = 5.8e-43
Identity = 130/375 (34.67%), Postives = 181/375 (48.27%), Query Frame = 0

Query: 10  PESLTF-PSSAVHDDSEIHL-HPSPMQQLQDASPRSTTSTINLSRIYYSKVLSSDSYKEM 69
           P   TF P+S      + HL HPSP          S  ST NLS    +    + SY ++
Sbjct: 2   PHCFTFKPASPEGSLGDDHLPHPSP--------EGSVASTFNLSH-ELAHAFQTPSYHDI 61

Query: 70  QRITSSLDLVAAAADQDHGSSHHDMLTQVLRPVPDHVHTA--LHRSKSNSLSNLISSYFQ 129
           +     +D      +          L+Q LRP  + V  A  L  +K  +L+NL+S+YFQ
Sbjct: 62  RSRLLVIDPTQENLEL--------FLSQELRPNNESVQEALSLRHAKQTTLTNLVSTYFQ 121

Query: 130 YSEETTSLCLTLRRSVAEAR-RLYCPI-----------HHLPTDSDCDLVFQTLVKFNEE 189
           +SE+ T  CL L ++V  AR  LY P+           H    +S C+L F   +K +  
Sbjct: 122 HSEDATRFCLNLYQNVHSARCHLYTPLLDLFNIFPRDSHSAIDESFCNLAFDVFLKLDTF 181

Query: 190 PNPFPSPNSQTFHEIRNLLCDLKGQLERRLRKSGSRIRLVRPPAAE-------------- 249
            NPF SP S +F + +     L  +L+ R+RKS SR+RL+    A               
Sbjct: 182 ENPFASPESHSFQDTQLCFYQLADKLDTRIRKSKSRVRLLHHATAGSALCLVTAVVVVAA 241

Query: 250 ----------PNSICCGGAAFAAYIPPKFVRRELAYAAQLKVASNNTFVLKTDLDTLDSL 309
                     P  +   G     Y+P  F ++EL+   QL VA+  TF L  DLDT+D L
Sbjct: 242 SAAFIAYHALPTILVVAGPLCTPYLPHSFKKKELSNIFQLNVAAKGTFALNKDLDTIDRL 301

Query: 310 VGRLHNAVEDDKRFIRLGLNIGNDEHTVQEVLIQLRKNHQNLGPYLDLLEQKICTCFTTV 345
           V RLH  V++DK  IRLGL  G D +T+ E + QLRK+H N    L++L   IC  FT V
Sbjct: 302 VSRLHTGVKNDKLLIRLGLERGRDVYTIPEFVKQLRKSHVNHTHQLEVLVDHICRWFTNV 359

BLAST of Lag0027428 vs. TAIR 10
Match: AT1G20180.2 (Protein of unknown function (DUF677) )

HSP 1 Score: 67.4 bits (163), Expect = 2.6e-11
Identity = 90/342 (26.32%), Postives = 135/342 (39.47%), Query Frame = 0

Query: 42  RSTTSTINLSRIYYSKVLSSDSYKEMQR-------ITSSLDLVAAAADQDHGS--SHHDM 101
           RS TS ++++   Y +   ++SY E +        ITS   L +++      S  S H  
Sbjct: 28  RSLTSKLSVNE-EYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSDLSFHSH 87

Query: 102 LTQVLRPVPDHVHTALHRSKSNSLSNLISSYFQYSEETTSLCLTL--------------- 161
            T  L   P     AL   + +SL NLI ++F  S E   +C TL               
Sbjct: 88  FTDYLLDPPQETLDAL--MQDSSLDNLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIK 147

Query: 162 ------RRSVAEARRLYCPIHHLPTDSDCDLVFQTLVKFNEEPNPF-PSPNSQTFHEIRN 221
                 +R    A+ L C    L     C L+FQ L +F    NP     N   F  + +
Sbjct: 148 RVMKIGKRVCNGAKTLECSPEML-----CALIFQELSRFAALKNPLCRIVNEAQFRIVHD 207

Query: 222 LLCDLKGQL---ERRLRKSGSRIRLVRPPAAEPNSICCGGAAFAAYIPPKFVRRE----- 281
              DL  +L   +RR+R+  S + +   PA        G  +F      K   R      
Sbjct: 208 ANSDLLTKLTSKKRRIRRKISILGVFAAPA------LLGLCSFCLLRKKKAKGRMHKSNK 267

Query: 282 ----LAYAAQLKVASNNTFVLKTDLDTLDSLVGRLHNAVEDDKRFIRLGLNIGNDEHTVQ 341
                    Q+ +A+   F+L  DLDTL  L GRL + +E  K    +       E  ++
Sbjct: 268 DTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRKTVAAMCAKSRKIE-VLK 327

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008449985.16.8e-11064.31PREDICTED: UPF0496 protein At3g19330 isoform X1 [Cucumis melo][more]
XP_022983457.12.0e-10963.71UPF0496 protein At3g19330 [Cucurbita maxima][more]
XP_004145706.12.6e-10963.66UPF0496 protein At3g19330 [Cucumis sativus][more]
KAE8650801.12.6e-10963.66hypothetical protein Csa_017445 [Cucumis sativus][more]
KAG6581140.13.4e-10963.81UPF0496 protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017876.1 U... [more]
Match NameE-valueIdentityDescription
Q9LT841.3e-4436.34UPF0496 protein At3g19330 OS=Arabidopsis thaliana OX=3702 GN=At3g19330 PE=2 SV=2[more]
Q9LJK48.2e-4234.67UPF0496 protein At3g19250 OS=Arabidopsis thaliana OX=3702 GN=At3g19250 PE=2 SV=1[more]
A2YH258.8e-1223.33Putative UPF0496 protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_023618 ... [more]
Q5Z8N68.8e-1223.33Putative UPF0496 protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0718... [more]
Q9SMU41.1e-0925.87UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BP813.3e-11064.31UPF0496 protein At3g19330 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491707 PE=... [more]
A0A6J1IZC99.5e-11063.71UPF0496 protein At3g19330 OS=Cucurbita maxima OX=3661 GN=LOC111482055 PE=3 SV=1[more]
A0A0A0L8I41.2e-10963.66Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G600010 PE=4 SV=1[more]
A0A6J1F7D73.6e-10963.43UPF0496 protein At3g19330-like OS=Cucurbita moschata OX=3662 GN=LOC111441533 PE=... [more]
A0A1S3BMN51.4e-10868.29UPF0496 protein At3g19330 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491707 PE=... [more]
Match NameE-valueIdentityDescription
AT3G19330.31.0e-4738.44Protein of unknown function (DUF677) [more]
AT3G19330.21.0e-4738.44Protein of unknown function (DUF677) [more]
AT3G19330.19.6e-4636.34Protein of unknown function (DUF677) [more]
AT3G19250.15.8e-4334.67Protein of unknown function (DUF677) [more]
AT1G20180.22.6e-1126.32Protein of unknown function (DUF677) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007749Protein of unknown function DUF677PANTHERPTHR31113UPF0496 PROTEIN 3-RELATEDcoord: 35..344
NoneNo IPR availablePANTHERPTHR31113:SF5OS04G0405700 PROTEINcoord: 35..344

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0027428.1Lag0027428.1mRNA