Lag0027331 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0027331
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	PAN domain-containing protein
Location	chr10: 46762774 .. 46763373 (+)
RNA-Seq Expression	Lag0027331
Synteny	Lag0027331

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGTAGCTGCTGCTCCGTTTTGTTTTGTCGTTCTTTTGTTGAATCTGGGCCGTACGATTTGCAGAACTGATATTGTTGCTGGTTACGAGGTTTCTCTGGCAGTGCCGGCGGAGTACGGCGAGGGGTTCATCGGAAGGGCCTTTTTGATCGAGACGGAGCATTTAATGCCGCCCAATTTCCGAGCAGCTTTGACCGTCGAAGCGACACAGGGCAACTTTTCGTGTTCGTTGCAGGTTTTTCTCGGAGAAGTTAAGGTTTGGAGCTCCGGCCACTTCTCCCGGTTTTTCACGGCGGAGAAATGCGTTCTTGAGCTGACCGGCGACGGCGATTTGAGGCTTAAGGGCCCCACCGGCCACGTGGGGTGGCGGACGGGGACTTCCGGACAAGGTGTGGAGGTACATGAAACGATGTCGTTTAACCCTTCTCTTGTATGGTTGTTGAATGAAGCGTTGTCTCAACTCAATGACTGTGTTTTGTTGATGCAGAGGCTTAGATTATTGAGAAGTGGGAATTTGGCGTTGGTGGATGCTTTGGAAGGAATTAAATGGCAGAGTTTCAATTTCCCAACGGACGTTATGCTTTTGGGCAGAGTTTAA

mRNA sequence

ATGAAGGTAGCTGCTGCTCCGTTTTGTTTTGTCGTTCTTTTGTTGAATCTGGGCCGTACGATTTGCAGAACTGATATTGTTGCTGGTTACGAGGTTTCTCTGGCAGTGCCGGCGGAGTACGGCGAGGGGTTCATCGGAAGGGCCTTTTTGATCGAGACGGAGCATTTAATGCCGCCCAATTTCCGAGCAGCTTTGACCGTCGAAGCGACACAGGGCAACTTTTCGTGTTCGTTGCAGGTTTTTCTCGGAGAAGTTAAGGTTTGGAGCTCCGGCCACTTCTCCCGGTTTTTCACGGCGGAGAAATGCGTTCTTGAGCTGACCGGCGACGGCGATTTGAGGCTTAAGGGCCCCACCGGCCACGTGGGGTGGCGGACGGGGACTTCCGGACAAGGTGTGGAGAGGCTTAGATTATTGAGAAGTGGGAATTTGGCGTTGGTGGATGCTTTGGAAGGAATTAAATGGCAGAGTTTCAATTTCCCAACGGACGTTATGCTTTTGGGCAGAGTTTAA

Coding sequence (CDS)

ATGAAGGTAGCTGCTGCTCCGTTTTGTTTTGTCGTTCTTTTGTTGAATCTGGGCCGTACGATTTGCAGAACTGATATTGTTGCTGGTTACGAGGTTTCTCTGGCAGTGCCGGCGGAGTACGGCGAGGGGTTCATCGGAAGGGCCTTTTTGATCGAGACGGAGCATTTAATGCCGCCCAATTTCCGAGCAGCTTTGACCGTCGAAGCGACACAGGGCAACTTTTCGTGTTCGTTGCAGGTTTTTCTCGGAGAAGTTAAGGTTTGGAGCTCCGGCCACTTCTCCCGGTTTTTCACGGCGGAGAAATGCGTTCTTGAGCTGACCGGCGACGGCGATTTGAGGCTTAAGGGCCCCACCGGCCACGTGGGGTGGCGGACGGGGACTTCCGGACAAGGTGTGGAGAGGCTTAGATTATTGAGAAGTGGGAATTTGGCGTTGGTGGATGCTTTGGAAGGAATTAAATGGCAGAGTTTCAATTTCCCAACGGACGTTATGCTTTTGGGCAGAGTTTAA

Protein sequence

MKVAAAPFCFVVLLLNLGRTICRTDIVAGYEVSLAVPAEYGEGFIGRAFLIETEHLMPPNFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTGDGDLRLKGPTGHVGWRTGTSGQGVERLRLLRSGNLALVDALEGIKWQSFNFPTDVMLLGRV

Homology

[Close]

BLAST of Lag0027331 vs. NCBI nr
Match: XP_022971607.1 (PAN domain-containing protein At5g03700 [Cucurbita maxima])

HSP 1 Score: 313.2 bits (801), Expect = 1.4e-81
Identity = 151/169 (89.35%), Postives = 162/169 (95.86%), Query Frame = 0


Query: 1   MKVA-AAPFCFVVLLLNLGRTICRTDIVAGYEVSLAVPAEYGEGFIGRAFLIETEHLMPP 60
           MKVA AA  CFV+LLLNL RTICRTDIV G++V+LAVPAEYGEGFIGRAFLIETEHL PP
Sbjct: 1   MKVASAAQICFVLLLLNLDRTICRTDIVPGHDVTLAVPAEYGEGFIGRAFLIETEHLPPP 60

Query: 61  NFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTGDGDLRLKGPTG 120
           NFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELT DGDLRLKGPTG
Sbjct: 61  NFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTDDGDLRLKGPTG 120

Query: 121 HVGWRTGTSGQGVERLRLLRSGNLALVDALEGIKWQSFNFPTDVMLLGR 169
           HVGWRTGT+GQGVE+LR+LRSGNLALVDAL+G+KWQSFNFPTDV+LLG+
Sbjct: 121 HVGWRTGTAGQGVEKLRILRSGNLALVDALDGVKWQSFNFPTDVLLLGQ 169

[Close]

BLAST of Lag0027331 vs. NCBI nr
Match: KAG6596316.1 (putative receptor protein kinase ZmPK1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027869.1 putative receptor protein kinase ZmPK1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 311.2 bits (796), Expect = 5.4e-81
Identity = 150/169 (88.76%), Postives = 161/169 (95.27%), Query Frame = 0


Query: 1   MKV-AAAPFCFVVLLLNLGRTICRTDIVAGYEVSLAVPAEYGEGFIGRAFLIETEHLMPP 60
           MKV +AA  CFV+LLLNL R ICRTDIV G+EV+LAVPAEYGEGFIGRAFLIETEHL PP
Sbjct: 1   MKVGSAAQICFVLLLLNLDRAICRTDIVPGHEVTLAVPAEYGEGFIGRAFLIETEHLPPP 60

Query: 61  NFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTGDGDLRLKGPTG 120
           NFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELT DGDLRLKGPTG
Sbjct: 61  NFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTDDGDLRLKGPTG 120

Query: 121 HVGWRTGTSGQGVERLRLLRSGNLALVDALEGIKWQSFNFPTDVMLLGR 169
           HVGWRTGT+GQGVE+LR+LRSGNLALVDAL+G+KWQSFNFPTDV+LLG+
Sbjct: 121 HVGWRTGTAGQGVEKLRILRSGNLALVDALDGVKWQSFNFPTDVLLLGQ 169

[Close]

BLAST of Lag0027331 vs. NCBI nr
Match: XP_022933279.1 (EP1-like glycoprotein 4 [Cucurbita moschata])

HSP 1 Score: 311.2 bits (796), Expect = 5.4e-81
Identity = 150/169 (88.76%), Postives = 161/169 (95.27%), Query Frame = 0


Query: 1   MKV-AAAPFCFVVLLLNLGRTICRTDIVAGYEVSLAVPAEYGEGFIGRAFLIETEHLMPP 60
           MKV +AA  CFV+LLLNL R ICRTDIV G+EV+LAVPAEYGEGFIGRAFLIETEHL PP
Sbjct: 1   MKVGSAAQICFVLLLLNLDRAICRTDIVPGHEVTLAVPAEYGEGFIGRAFLIETEHLPPP 60

Query: 61  NFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTGDGDLRLKGPTG 120
           NFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELT DGDLRLKGPTG
Sbjct: 61  NFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTDDGDLRLKGPTG 120

Query: 121 HVGWRTGTSGQGVERLRLLRSGNLALVDALEGIKWQSFNFPTDVMLLGR 169
           HVGWRTGT+GQGVE+LR+LRSGNLALVDAL+G+KWQSFNFPTDV+LLG+
Sbjct: 121 HVGWRTGTAGQGVEKLRILRSGNLALVDALDGVKWQSFNFPTDVLLLGQ 169

[Close]

BLAST of Lag0027331 vs. NCBI nr
Match: XP_023540139.1 (EP1-like glycoprotein 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 310.8 bits (795), Expect = 7.0e-81
Identity = 150/169 (88.76%), Postives = 161/169 (95.27%), Query Frame = 0


Query: 1   MKV-AAAPFCFVVLLLNLGRTICRTDIVAGYEVSLAVPAEYGEGFIGRAFLIETEHLMPP 60
           MKV +AA  CFV+LLLNL RTICRTDIV G+EV+LAVPAEYGEGFIGRAFLIETEHL PP
Sbjct: 1   MKVGSAAQICFVLLLLNLDRTICRTDIVPGHEVTLAVPAEYGEGFIGRAFLIETEHLPPP 60

Query: 61  NFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTGDGDLRLKGPTG 120
           NFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELT DGDLRLKGPTG
Sbjct: 61  NFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTDDGDLRLKGPTG 120

Query: 121 HVGWRTGTSGQGVERLRLLRSGNLALVDALEGIKWQSFNFPTDVMLLGR 169
           HVGWRTGT+GQGVE+LR+LRSGNLALVDA +G+KWQSFNFPTDV+LLG+
Sbjct: 121 HVGWRTGTAGQGVEKLRILRSGNLALVDASDGVKWQSFNFPTDVLLLGQ 169

[Close]

BLAST of Lag0027331 vs. NCBI nr
Match: XP_038903590.1 (EP1-like glycoprotein 3 [Benincasa hispida])

HSP 1 Score: 289.7 bits (740), Expect = 1.7e-74
Identity = 141/169 (83.43%), Postives = 152/169 (89.94%), Query Frame = 0


Query: 1   MKVAAAPFCF-VVLLLNLGRTICRTDIVAGYEVSLAVPAEYGEGFIGRAFLIETEHLMPP 60
           +KVAA  FCF ++L  NLG +ICRTDIV GYEV+L VPAEY E FIGRAFL+ETEHLMPP
Sbjct: 29  LKVAAG-FCFLIILFFNLGPSICRTDIVTGYEVNLVVPAEYEEWFIGRAFLMETEHLMPP 88

Query: 61  NFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTGDGDLRLKGPTG 120
           NFR ALTVEATQG +SCSLQVFLGEV+VWSSGHFSRFFTAEKCVLELT DGDLRLKGPTG
Sbjct: 89  NFRVALTVEATQGQYSCSLQVFLGEVRVWSSGHFSRFFTAEKCVLELTADGDLRLKGPTG 148

Query: 121 HVGWRTGTSGQGVERLRLLRSGNLALVDALEGIKWQSFNFPTDVMLLGR 169
           HVGWRTGTS QGVERLR+LRSGNLALVDA E  KWQSFNFPTDVM+LG+
Sbjct: 149 HVGWRTGTSRQGVERLRILRSGNLALVDAFERTKWQSFNFPTDVMVLGQ 196

[Close]

BLAST of Lag0027331 vs. ExPASy Swiss-Prot
Match: Q39688 (Epidermis-specific secreted glycoprotein EP1 OS=Daucus carota OX=4039 GN=EP1 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 7.5e-09
Identity = 28/69 (40.58%), Postives = 41/69 (59.42%), Query Frame = 0


Query: 100 EKCVLELTGDGDLRLKGPTGHVGWRTGTSGQGVERLRLLRSGNLALVDALEGIKWQSFNF 159
           E   L    DG+L L    G V W+T T+ +GV  L++L +GN+ L D+     WQSF+ 
Sbjct: 102 ENATLTFGPDGNLVLARSNGQVAWQTSTANKGVVGLKILPNGNMVLYDSKGKFLWQSFDT 161

Query: 160 PTDVMLLGR 169
           PTD +L+G+
Sbjct: 162 PTDTLLVGQ 170

[Close]

BLAST of Lag0027331 vs. ExPASy Swiss-Prot
Match: Q9ZVA5 (EP1-like glycoprotein 4 OS=Arabidopsis thaliana OX=3702 GN=At1g78860 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 2.8e-08
Identity = 29/82 (35.37%), Postives = 45/82 (54.88%), Query Frame = 0


Query: 87  VWSSGHFSRFFTAEKCVLELTGDGDLRLKGPTGHVGWRTGTSGQGVERLRLLRSGNLALV 146
           VW +   S     E   L    DG+L L    G V W+T T+ +GV  +++L +GN+ + 
Sbjct: 90  VWEANRGSP--VKENATLTFGEDGNLVLAEADGRVVWQTNTANKGVVGIKILENGNMVIY 149

Query: 147 DALEGIKWQSFNFPTDVMLLGR 169
           D+     WQSF+ PTD +L+G+
Sbjct: 150 DSNGKFVWQSFDSPTDTLLVGQ 169

[Close]

BLAST of Lag0027331 vs. ExPASy Swiss-Prot
Match: Q9ZVA4 (EP1-like glycoprotein 3 OS=Arabidopsis thaliana OX=3702 GN=At1g78850 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.4e-07
Identity = 27/82 (32.93%), Postives = 44/82 (53.66%), Query Frame = 0


Query: 87  VWSSGHFSRFFTAEKCVLELTGDGDLRLKGPTGHVGWRTGTSGQGVERLRLLRSGNLALV 146
           VW +   S     E   L    DG+L L    G + W+T T+ +G   +++L +GN+ + 
Sbjct: 90  VWEANRGSP--VKENATLTFGEDGNLVLAEADGRLVWQTNTANKGAVGIKILENGNMVIY 149

Query: 147 DALEGIKWQSFNFPTDVMLLGR 169
           D+     WQSF+ PTD +L+G+
Sbjct: 150 DSSGKFVWQSFDSPTDTLLVGQ 169

[Close]

BLAST of Lag0027331 vs. ExPASy Swiss-Prot
Match: Q9ZVA2 (EP1-like glycoprotein 2 OS=Arabidopsis thaliana OX=3702 GN=At1g78830 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.4e-07
Identity = 27/69 (39.13%), Postives = 39/69 (56.52%), Query Frame = 0


Query: 100 EKCVLELTGDGDLRLKGPTGHVGWRTGTSGQGVERLRLLRSGNLALVDALEGIKWQSFNF 159
           E   L L  +G+L L    G V W+T T+ +GV   ++L +GN+ L D      WQSF+ 
Sbjct: 105 ENATLSLGRNGNLVLAEADGRVKWQTNTANKGVTGFQILPNGNIVLHDKNGKFVWQSFDH 164

Query: 160 PTDVMLLGR 169
           PTD +L G+
Sbjct: 165 PTDTLLTGQ 173

[Close]

BLAST of Lag0027331 vs. ExPASy Swiss-Prot
Match: Q9ZVA1 (EP1-like glycoprotein 1 OS=Arabidopsis thaliana OX=3702 GN=At1g78820 PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.6e-06
Identity = 25/69 (36.23%), Postives = 39/69 (56.52%), Query Frame = 0


Query: 100 EKCVLELTGDGDLRLKGPTGHVGWRTGTSGQGVERLRLLRSGNLALVDALEGIKWQSFNF 159
           +   L    +G+L L    G V W+T T+ +GV   ++L +GN+ L D      WQSF+ 
Sbjct: 105 DNSTLSFGRNGNLVLAELNGQVKWQTNTANKGVTGFQILPNGNMVLHDKHGKFVWQSFDH 164

Query: 160 PTDVMLLGR 169
           PTD +L+G+
Sbjct: 165 PTDTLLVGQ 173

The following BLAST results are available for this feature:

BLAST of Lag0027331 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022971607.1	1.4e-81	89.35	PAN domain-containing protein At5g03700 [Cucurbita maxima]	[more]
KAG6596316.1	5.4e-81	88.76	putative receptor protein kinase ZmPK1, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022933279.1	5.4e-81	88.76	EP1-like glycoprotein 4 [Cucurbita moschata]	[more]
XP_023540139.1	7.0e-81	88.76	EP1-like glycoprotein 4 [Cucurbita pepo subsp. pepo]	[more]
XP_038903590.1	1.7e-74	83.43	EP1-like glycoprotein 3 [Benincasa hispida]	[more]

BLAST of Lag0027331 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39688	7.5e-09	40.58	Epidermis-specific secreted glycoprotein EP1 OS=Daucus carota OX=4039 GN=EP1 PE=...	[more]
Q9ZVA5	2.8e-08	35.37	EP1-like glycoprotein 4 OS=Arabidopsis thaliana OX=3702 GN=At1g78860 PE=3 SV=1	[more]
Q9ZVA4	1.4e-07	32.93	EP1-like glycoprotein 3 OS=Arabidopsis thaliana OX=3702 GN=At1g78850 PE=1 SV=1	[more]
Q9ZVA2	2.4e-07	39.13	EP1-like glycoprotein 2 OS=Arabidopsis thaliana OX=3702 GN=At1g78830 PE=1 SV=1	[more]
Q9ZVA1	1.6e-06	36.23	EP1-like glycoprotein 1 OS=Arabidopsis thaliana OX=3702 GN=At1g78820 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001480	Bulb-type lectin domain	SMART	SM00108	blect_4	coord: 47..160 e-value: 5.6E-4 score: 24.5
IPR001480	Bulb-type lectin domain	PFAM	PF01453	B_lectin	coord: 98..166 e-value: 1.9E-8 score: 34.7
IPR001480	Bulb-type lectin domain	PROSITE	PS50927	BULB_LECTIN	coord: 41..158 score: 9.218224
IPR036426	Bulb-type lectin domain superfamily	GENE3D	2.90.10.10		coord: 46..158 e-value: 3.3E-11 score: 45.2
IPR036426	Bulb-type lectin domain superfamily	SUPERFAMILY	51110	alpha-D-mannose-specific plant lectins	coord: 86..162
None	No IPR available	PANTHER	PTHR32444	FAMILY NOT NAMED	coord: 8..168
None	No IPR available	PANTHER	PTHR32444:SF6	D-MANNOSE BINDING LECTIN PROTEIN WITH APPLE-LIKE CARBOHYDRATE-BINDING DOMAIN-CONTAINING PROTEIN	coord: 8..168

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0027331.1	Lag0027331.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016301	kinase activity