Lag0027257 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0027257 vs. NCBI nr
Match: XP_038904775.1 (vacuolar cation/proton exchanger 3-like isoform X2 [Benincasa hispida] >XP_038904777.1 vacuolar cation/proton exchanger 3-like isoform X2 [Benincasa hispida])

HSP 1 Score: 729.6 bits (1882), Expect = 1.6e-206
Identity = 384/433 (88.68%), Postives = 402/433 (92.84%), Query Frame = 0

Query: 1   MDFEQGTSILQNGAVKGSLRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQEV 60
           MDFE GTSILQNGAVKGSLRH RT HNMSSSSLRK+SDLTLV KVRFG LR LLVNLQEV
Sbjct: 1   MDFEPGTSILQNGAVKGSLRHARTAHNMSSSSLRKKSDLTLVPKVRFGCLRQLLVNLQEV 60

Query: 61  ILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPTVG 120
           I GTRIWILFPA+PLAVLA+SFGF RPWVFVLSL+GLAPLAERLSFLTEQISYFTG TVG
Sbjct: 61  IFGTRIWILFPAVPLAVLADSFGFPRPWVFVLSLLGLAPLAERLSFLTEQISYFTGHTVG 120

Query: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNKEQ 180
           GLLNATCGNATELI+A+FALSK KI+LVKYSLLGSVLSNLLLVLGTSLF GGIVNL KEQ
Sbjct: 121 GLLNATCGNATELIVAVFALSKDKIKLVKYSLLGSVLSNLLLVLGTSLFFGGIVNLKKEQ 180

Query: 181 TYDRRQAEVNAHMLLLALLCHSLPMIFPYAGGSADVAGAGALRLSRAGSIVMLVAYVAYL 240
           TYD RQAEVN+HMLLLALLCHSLPM+FPYAGGSADVA    LRLSRAGSI+ML+AYVAYL
Sbjct: 181 TYDGRQAEVNSHMLLLALLCHSLPMLFPYAGGSADVAEGSVLRLSRAGSIIMLLAYVAYL 240

Query: 241 IFQLWTHRRLFEPEKEPDEYDDVVEEAPVIGIWSAMAWLVGSTLLIALLSEYVVATIEDA 300
           IF LW HRRLFE +KE DEYDDVVEE PVIG WSAMAWLV  TLLIALLSEYVV TIEDA
Sbjct: 241 IFHLWNHRRLFEEDKELDEYDDVVEEGPVIGKWSAMAWLVVVTLLIALLSEYVVTTIEDA 300

Query: 301 SETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPLCV 360
           SETWGLSVAFLSIILLP++GNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPLCV
Sbjct: 301 SETWGLSVAFLSIILLPMIGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPLCV 360

Query: 361 IVAWAMGMNMDLDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFYIFISTCFFLT 420
           IVAW MGMNMDL+FNILET SLALSI+ASSSILQDGTSHYMKGLILLLFYI I+TCFFLT
Sbjct: 361 IVAWVMGMNMDLNFNILETASLALSILASSSILQDGTSHYMKGLILLLFYIVIATCFFLT 420

Query: 421 NDEVTIINSGLSR 434
           NDEVT+ N GLSR
Sbjct: 421 NDEVTLNNFGLSR 433

BLAST of Lag0027257 vs. NCBI nr
Match: XP_023539286.1 (vacuolar cation/proton exchanger 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 700.3 bits (1806), Expect = 1.1e-197
Identity = 373/437 (85.35%), Postives = 400/437 (91.53%), Query Frame = 0

Query: 1   MDFEQGTSILQNGAVKGSLRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQEV 60
           MDFEQ T ILQNGAVK SL  GRT HNMSSSSLR++SDLTLVSKVRFG LRLLLVNLQEV
Sbjct: 1   MDFEQETPILQNGAVKSSL-IGRTAHNMSSSSLRRKSDLTLVSKVRFGCLRLLLVNLQEV 60

Query: 61  ILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPTVG 120
           ILGT+IW LFPAIPLAVLA+S GFGR WVFVLSL+GLAPLAERLSFLTEQISYFTGPT+G
Sbjct: 61  ILGTKIWFLFPAIPLAVLADSLGFGRAWVFVLSLLGLAPLAERLSFLTEQISYFTGPTIG 120

Query: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNKEQ 180
           GLLNATCGNATELIIAIFALSK KIELVKYSLLGSVLSNLL+VLGTSLFIGGIVNL ++Q
Sbjct: 121 GLLNATCGNATELIIAIFALSKDKIELVKYSLLGSVLSNLLVVLGTSLFIGGIVNLKRQQ 180

Query: 181 TYDRRQAEVNAHMLLLALLCHSLPMIFPYAGGSADVAGAGALRLSRAGSIVMLVAYVAYL 240
           TYD+RQAEVN+HMLLLALLCH LPM+FPY  GS DV  A ALRLSRA SIVMLVAYVAYL
Sbjct: 181 TYDQRQAEVNSHMLLLALLCHLLPMLFPYVRGSTDVVVADALRLSRASSIVMLVAYVAYL 240

Query: 241 IFQLWTHRRLFEPEKEPDEY-DDVVEEAPVIGIWSAMAWLVGSTLLIALLSEYVVATIED 300
           IFQLWTHR  FEP+KE D Y DDVV+EAP IGIWS +AWLVG T+LIALLSEYVVATIED
Sbjct: 241 IFQLWTHRGFFEPDKELDVYNDDVVQEAPTIGIWSTVAWLVGVTVLIALLSEYVVATIED 300

Query: 301 ASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPLC 360
           AS++WGLSV FLS+ILLPLVGNAAEHAGA+IFAYKNKLDITLGVALGSATQIAMFVVPLC
Sbjct: 301 ASKSWGLSVTFLSMILLPLVGNAAEHAGALIFAYKNKLDITLGVALGSATQIAMFVVPLC 360

Query: 361 VIVAWAMGMNMDLDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFYIFISTCFFL 420
           VIV W MG+NMDL+FN+LET SLALSIMASSSILQDG SHYMKGLIL+LFYI I+ CFF 
Sbjct: 361 VIVGWIMGINMDLNFNVLETSSLALSIMASSSILQDGNSHYMKGLILILFYIIIAICFFF 420

Query: 421 TNDEVTIINSGLSRVQL 437
           TNDEVTI++S LSRVQL
Sbjct: 421 TNDEVTIVHSQLSRVQL 436

BLAST of Lag0027257 vs. NCBI nr
Match: XP_022971605.1 (vacuolar cation/proton exchanger 3-like [Cucurbita maxima])

HSP 1 Score: 698.0 bits (1800), Expect = 5.3e-197
Identity = 372/438 (84.93%), Postives = 399/438 (91.10%), Query Frame = 0

Query: 1   MDFEQGTSILQNGAVKGSLRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQEV 60
           MDFEQ T ILQNGAVKGSL  GRT HNMSSSSLR++SDLTLVSKVRFG LRLLLVNLQEV
Sbjct: 1   MDFEQETPILQNGAVKGSL-IGRTAHNMSSSSLRRKSDLTLVSKVRFGCLRLLLVNLQEV 60

Query: 61  ILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPTVG 120
           ILGT+IW LFPAIPLAVLA+S GFGR WVFVLSL+GLAPLAERLSFLTEQISYFTGPT+G
Sbjct: 61  ILGTKIWFLFPAIPLAVLADSLGFGRAWVFVLSLLGLAPLAERLSFLTEQISYFTGPTIG 120

Query: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNKEQ 180
           GLLNATCGNATELIIAIFALSK KIELVKYSLLGSVLSNLL+VLGTSLFI GIVNL ++Q
Sbjct: 121 GLLNATCGNATELIIAIFALSKDKIELVKYSLLGSVLSNLLVVLGTSLFISGIVNLKRQQ 180

Query: 181 TYDRRQAEVNAHMLLLALLCHSLPMIFPYAGGSADVAGAGALRLSRAGSIVMLVAYVAYL 240
           TYD+RQ EVN+HMLLLALLCH LPM+FPY  GS DV  A ALRLSRA SIVMLVAYVAYL
Sbjct: 181 TYDQRQVEVNSHMLLLALLCHLLPMLFPYVKGSTDVVVAKALRLSRASSIVMLVAYVAYL 240

Query: 241 IFQLWTHRRLFEPEKEPDEY-DDVVEEAPVIGIWSAMAWLVGSTLLIALLSEYVVATIED 300
           IFQLWTHR  FEP+KE D Y DDVV+EAP IGIWS +AWLVG T+LIALLSEYVVATIED
Sbjct: 241 IFQLWTHREFFEPDKELDVYNDDVVQEAPTIGIWSTVAWLVGVTILIALLSEYVVATIED 300

Query: 301 ASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPLC 360
           ASE+WGLSVAFLS+ILLPLVGNAAEHAGA+IFAYKNKLDITLGVALGSATQIAMFVVPLC
Sbjct: 301 ASESWGLSVAFLSMILLPLVGNAAEHAGALIFAYKNKLDITLGVALGSATQIAMFVVPLC 360

Query: 361 VIVAWAMGMNMDLDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFYIFISTCFFL 420
           VIV W MG+NMDL  N+LET SLALSIMASSSILQDG+SHYMKGLIL+LFYI I+ CFF 
Sbjct: 361 VIVGWIMGINMDLKCNVLETASLALSIMASSSILQDGSSHYMKGLILILFYIIIAICFFF 420

Query: 421 TNDEVTIINSGLSRVQLE 438
           TNDEVTI++S  SRVQL+
Sbjct: 421 TNDEVTIVHSQSSRVQLK 437

BLAST of Lag0027257 vs. NCBI nr
Match: XP_022948688.1 (vacuolar cation/proton exchanger 3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 692.2 bits (1785), Expect = 2.9e-195
Identity = 369/438 (84.25%), Postives = 399/438 (91.10%), Query Frame = 0

Query: 1   MDFEQGTSILQNGAVKGSLRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQEV 60
           MDFEQ T ILQNGAVK SL  GRT HNMSSSSLR++SDLTLVSKVRFG LRLLLVNLQEV
Sbjct: 1   MDFEQETPILQNGAVKSSL-IGRTAHNMSSSSLRRKSDLTLVSKVRFGCLRLLLVNLQEV 60

Query: 61  ILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPTVG 120
           ILGT+IW LFPAIPLAVLA+S GFGR WVFVLSL+GLAPLAERLSFLTEQISYFTGPT+G
Sbjct: 61  ILGTKIWFLFPAIPLAVLADSLGFGRAWVFVLSLLGLAPLAERLSFLTEQISYFTGPTIG 120

Query: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNKEQ 180
           GLLNATCGNATELIIAIFALSK KIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNL ++Q
Sbjct: 121 GLLNATCGNATELIIAIFALSKDKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLKRQQ 180

Query: 181 TYDRRQAEVNAHMLLLALLCHSLPMIFPYAGGSADVAGAGALRLSRAGSIVMLVAYVAYL 240
           TYD+RQAEVN+HMLLLAL+CH LPM+FPY  GS DV  A ALRLSRA SIVMLVAYVAYL
Sbjct: 181 TYDQRQAEVNSHMLLLALVCHLLPMLFPYVRGSTDVVVAEALRLSRASSIVMLVAYVAYL 240

Query: 241 IFQLWTHRRLFEPEKEPDEY-DDVVEEAPVIGIWSAMAWLVGSTLLIALLSEYVVATIED 300
           IFQLWTH   FEP+KE + Y DDVV+EAP IGIWS +AWL G T+LIALLSEYVVATIED
Sbjct: 241 IFQLWTHCEFFEPDKELEVYNDDVVQEAPTIGIWSIVAWLGGVTILIALLSEYVVATIED 300

Query: 301 ASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPLC 360
            SE+WGLSVAFLS+ILLPLVGNAAEHAGA+IFAYKNKLDITLGVALGSATQIAMFVVPLC
Sbjct: 301 VSESWGLSVAFLSMILLPLVGNAAEHAGALIFAYKNKLDITLGVALGSATQIAMFVVPLC 360

Query: 361 VIVAWAMGMNMDLDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFYIFISTCFFL 420
           VIV W MG+NMDL+FN+LET SLALSIMASSSILQDG+SHYMKGLIL+LFYI I+ CFF 
Sbjct: 361 VIVGWIMGINMDLNFNVLETASLALSIMASSSILQDGSSHYMKGLILILFYIIIAICFFF 420

Query: 421 TNDEVTIINSGLSRVQLE 438
           T+DEV I++S LSRVQL+
Sbjct: 421 TSDEVIIVHSQLSRVQLK 437

BLAST of Lag0027257 vs. NCBI nr
Match: KAA0044167.1 (vacuolar cation/proton exchanger 3-like [Cucumis melo var. makuwa] >TYK24966.1 vacuolar cation/proton exchanger 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 691.8 bits (1784), Expect = 3.8e-195
Identity = 368/439 (83.83%), Postives = 398/439 (90.66%), Query Frame = 0

Query: 1   MDFEQG-TSILQN-GAVKGSLRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQ 60
           MDFE G TSILQN G VK S R  R  HNMSSSSLRK+SDLTLVSKVRFG LRL LVNLQ
Sbjct: 1   MDFEPGTTSILQNAGGVKSSQRLARMAHNMSSSSLRKKSDLTLVSKVRFGSLRLFLVNLQ 60

Query: 61  EVILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPT 120
           EVILGTRIWILFPA+PLAVLA+SFGF RPWVFV+SLMGLAPLAERLSFLTEQISYFTGPT
Sbjct: 61  EVILGTRIWILFPAVPLAVLADSFGFARPWVFVVSLMGLAPLAERLSFLTEQISYFTGPT 120

Query: 121 VGGLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNK 180
           VGGLLNATCGN TELI+AIFAL K KI+LVKYSLLGS+LSNLLLVLGTSLF GGI+NLNK
Sbjct: 121 VGGLLNATCGNVTELIVAIFALRKEKIKLVKYSLLGSLLSNLLLVLGTSLFFGGILNLNK 180

Query: 181 EQTYDRRQAEVNAHMLLLALLCHSLPMIFPYAGGSADVAGAGALRLSRAGSIVMLVAYVA 240
           +QT+D  QAEVN+HML+LALLCHSLPM+FPYAGGS DVAG GA+RLSRA SIVMLVAYVA
Sbjct: 181 QQTFDSSQAEVNSHMLMLALLCHSLPMLFPYAGGSVDVAGVGAVRLSRACSIVMLVAYVA 240

Query: 241 YLIFQLWTHRRLFEPEKEPDEYDDVVEEAPVIGIWSAMAWLVGSTLLIALLSEYVVATIE 300
           YLIFQLW+HRRLFE  +E D YDD +EE PVIG WSAMAWLV  TLL+ALLSEYVVATIE
Sbjct: 241 YLIFQLWSHRRLFEANEELDVYDDGLEEGPVIGKWSAMAWLVVVTLLVALLSEYVVATIE 300

Query: 301 DASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL 360
           DASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQI MFVVPL
Sbjct: 301 DASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIGMFVVPL 360

Query: 361 CVIVAWAMGMNMDLDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFYIFISTCFF 420
            VIVAW MG+NMDL+FNI+ET SLALS++ASSSILQDG SHYMKGLILLLFYI I+T FF
Sbjct: 361 SVIVAWVMGINMDLNFNIIETASLALSVLASSSILQDGNSHYMKGLILLLFYIVIATYFF 420

Query: 421 LTNDEVTIINSGLSRVQLE 438
           LT+D+VT+ N GLS ++L+
Sbjct: 421 LTSDDVTVNNLGLSSIKLK 439

BLAST of Lag0027257 vs. ExPASy Swiss-Prot
Match: Q93Z81 (Vacuolar cation/proton exchanger 3 OS=Arabidopsis thaliana OX=3702 GN=CAX3 PE=1 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 5.4e-152
Identity = 288/429 (67.13%), Postives = 352/429 (82.05%), Query Frame = 0

Query: 4   EQGTSILQNG----AVKGS---LRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVN 63
           E   +I +NG      KGS   LRHGRT HNMSSSSLRK+SDL LV KV    L+ +L N
Sbjct: 6   EPWAAIAENGNANVTAKGSSRELRHGRTAHNMSSSSLRKKSDLRLVQKVPCKTLKNILSN 65

Query: 64  LQEVILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTG 123
           LQEVILGT++ +LF AIPLA+LANS+ +GRP +F LSL+GL PLAER+SFLTEQ++++TG
Sbjct: 66  LQEVILGTKLTLLFLAIPLAILANSYNYGRPLIFGLSLIGLTPLAERVSFLTEQLAFYTG 125

Query: 124 PTVGGLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNL 183
           PTVGGLLNATCGNATELIIAI AL+ +K+ +VKYSLLGS+LSNLLLVLGTSLF GGI N+
Sbjct: 126 PTVGGLLNATCGNATELIIAILALANNKVAVVKYSLLGSILSNLLLVLGTSLFFGGIANI 185

Query: 184 NKEQTYDRRQAEVNAHMLLLALLCHSLPMIFPYAGG---SADVAGAGALRLSRAGSIVML 243
            +EQ +DR+QA+VN  +LL+ LLCH LP++  YA     S  +    +L LSR  SIVML
Sbjct: 186 RREQRFDRKQADVNFFLLLMGLLCHLLPLLLKYAATGEVSTSMINKMSLTLSRTSSIVML 245

Query: 244 VAYVAYLIFQLWTHRRLFEPEKEPDE-YDD--VVEEAPVIGIWSAMAWLVGSTLLIALLS 303
           +AY+AYLIFQLWTHR+LFE +++ D+ YDD   VEE PVIG WS  AWLVG T++IALLS
Sbjct: 246 IAYIAYLIFQLWTHRQLFEAQQDDDDAYDDEVSVEETPVIGFWSGFAWLVGMTIVIALLS 305

Query: 304 EYVVATIEDASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQ 363
           EYVV TIEDAS++WGLSV+F+SIILLP+VGNAAEHAGA+IFA+KNKLDI+LGVALGSATQ
Sbjct: 306 EYVVDTIEDASDSWGLSVSFISIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQ 365

Query: 364 IAMFVVPLCVIVAWAMGMNMDLDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFY 420
           I++FVVPL VIVAW +G+ MDL+FNILET SLAL+I+ ++  LQDGTSHYMKGL+LLL Y
Sbjct: 366 ISLFVVPLSVIVAWILGIKMDLNFNILETSSLALAIIITAFTLQDGTSHYMKGLVLLLCY 425

BLAST of Lag0027257 vs. ExPASy Swiss-Prot
Match: Q39253 (Vacuolar cation/proton exchanger 1 OS=Arabidopsis thaliana OX=3702 GN=CAX1 PE=1 SV=3)

HSP 1 Score: 514.6 bits (1324), Expect = 1.1e-144
Identity = 274/423 (64.78%), Postives = 338/423 (79.91%), Query Frame = 0

Query: 8   SILQNG----AVKGS---LRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQEV 67
           S+ +NG      KGS   LR GRT HNMSSSSLRK+SDL ++ KV + GL+  L NLQEV
Sbjct: 10  SVAENGNPSITAKGSSRELRLGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFLSNLQEV 69

Query: 68  ILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPTVG 127
           ILGT++ ILFPAIP A++    G  +PW+F LSL+GL PLAER+SFLTEQ++++TGPT+G
Sbjct: 70  ILGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFYTGPTLG 129

Query: 128 GLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNKEQ 187
           GLLNATCGNATELIIAI AL+ +K+ +VKYSLLGS+LSNLLLVLGTSLF GGI N+ +EQ
Sbjct: 130 GLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIANIRREQ 189

Query: 188 TYDRRQAEVNAHMLLLALLCHSLPMIFPY---AGGSADVAGAGALRLSRAGSIVMLVAYV 247
            +DR+QA+VN  +LLL  LCH LP++  Y      SA V     L +SR  SIVML++Y+
Sbjct: 190 RFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIVMLISYI 249

Query: 248 AYLIFQLWTHRRLFEPEKEPDEYDDVVE-EAPVIGIWSAMAWLVGSTLLIALLSEYVVAT 307
           AYL+FQLWTHR+LF+ +++ DEYDD VE E  VI  WS  AWLVG TL+IALLSEYVVAT
Sbjct: 250 AYLVFQLWTHRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLSEYVVAT 309

Query: 308 IEDASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVV 367
           IE+AS+ W LSV+F+SIILLP+VGNAAEHAGAVIFA+KNKLDI+LGVALGSATQI +FVV
Sbjct: 310 IEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLDISLGVALGSATQIGLFVV 369

Query: 368 PLCVIVAWAMGMNMDLDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFYIFISTC 420
           PL +IVAW +G+NMDL+F  LET  LA+SI+ ++  LQDG+SHYMKGL+LLL Y  I+ C
Sbjct: 370 PLTIIVAWILGINMDLNFGPLETGCLAVSIIITAFTLQDGSSHYMKGLVLLLCYFIIAIC 429

BLAST of Lag0027257 vs. ExPASy Swiss-Prot
Match: Q769E5 (Vacuolar cation/proton exchanger 1a OS=Oryza sativa subsp. japonica OX=39947 GN=CAX1a PE=1 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 7.8e-135
Identity = 265/418 (63.40%), Postives = 320/418 (76.56%), Query Frame = 0

Query: 21  HG--RTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQEVILGTRIWILFPAIPLAVL 80
           HG  RT HNMSSSSLRK+SD  LV KV    LR LL NLQEV L T++ +LFPA+PLA+ 
Sbjct: 19  HGRSRTAHNMSSSSLRKKSDAALVRKVPVAPLRPLLANLQEVFLATKLAVLFPAVPLAIA 78

Query: 81  ANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPTVGGLLNATCGNATELIIAIF 140
           A  F F + WVF LSL+GL PLAER+SFLTEQI+ +TGPTVGGLLNATCGNATELIIA+F
Sbjct: 79  AQCFRFDQVWVFALSLLGLIPLAERVSFLTEQIALYTGPTVGGLLNATCGNATELIIALF 138

Query: 141 ALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNKEQTYDRRQAEVNAHMLLLAL 200
           AL K KIE+VK SLLGSVLSNLLLVLGTSLF GG+VNL   Q YDR Q++V+  +L LA+
Sbjct: 139 ALLKGKIEVVKCSLLGSVLSNLLLVLGTSLFCGGVVNLGARQPYDRNQSDVSTALLFLAV 198

Query: 201 LCHSLPMIFPYAGGSAD-----VAGAGALRLSRAGSIVMLVAYVAYLIFQLWTHRRLFEP 260
           LCHS P++  YA  + +      + A +L LSRA S VML +YVAYL FQL THR+LFEP
Sbjct: 199 LCHSAPLLLRYAVAAGEHSVSATSAAASLDLSRACSFVMLASYVAYLFFQLKTHRQLFEP 258

Query: 261 EKEP--DEYDDVVEEAPVIGIWSAMAWLVGSTLLIALLSEYVVATIEDASETWGLSVAFL 320
           ++    D  DD  +E P +G  SA+ WL   T +I++LSEYVV TIE  S++WGLSV+F+
Sbjct: 259 QEVDGGDAGDD--DEEPALGFASALFWLALMTAVISVLSEYVVGTIEPTSQSWGLSVSFI 318

Query: 321 SIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPLCVIVAWAMGMNMD 380
           SIILLP+VGNAAEHAGA+IFA KNKLDITLGVALGSATQI+MFVVPL V+VAW MG+ MD
Sbjct: 319 SIILLPIVGNAAEHAGAIIFALKNKLDITLGVALGSATQISMFVVPLSVLVAWIMGVQMD 378

Query: 381 LDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFYIFISTCFFLTNDEVTIINS 430
           LDF +LET SL ++++ ++  LQDGTSHY+KG++LLL YI I  CFF+        NS
Sbjct: 379 LDFKLLETGSLFMAVLVTAFTLQDGTSHYLKGILLLLCYIVIGACFFVARQPAGHANS 434

BLAST of Lag0027257 vs. ExPASy Swiss-Prot
Match: Q5TKG3 (Vacuolar cation/proton exchanger 1b OS=Oryza sativa subsp. japonica OX=39947 GN=CAX1b PE=2 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 3.0e-134
Identity = 265/404 (65.59%), Postives = 323/404 (79.95%), Query Frame = 0

Query: 23  RTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQEVILGTRIWILFPAIPLAVLANSF 82
           RT HN+SSSSLRK SD +L+ KV    LR LL NL +V+L TR+++LFPA+ LA+ A   
Sbjct: 31  RTAHNLSSSSLRKSSDASLLHKVPCAALRSLLANLNDVLLTTRLFLLFPAVLLAIAATYL 90

Query: 83  GFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPTVGGLLNATCGNATELIIAIFALSK 142
            FG+ WVFVLSL+GL PLAERLSFLTEQI+++TGPTVGGLLNAT GN TE+IIA+ AL +
Sbjct: 91  HFGQVWVFVLSLIGLVPLAERLSFLTEQIAFYTGPTVGGLLNATFGNVTEVIIALLALRE 150

Query: 143 HKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNKEQTYDRRQAEVNAHMLLLALLCHS 202
            KIE+VK SLLGS+LSNLLLVLGTSLF+ GI NL   Q YD +QA VN  +L+LA+LCHS
Sbjct: 151 GKIEVVKCSLLGSILSNLLLVLGTSLFLAGIANLRAHQPYDTKQAHVNTALLMLAVLCHS 210

Query: 203 LPMIFPYA---GGSADVAGAGALRLSRAGSIVMLVAYVAYLIFQLWTHRRLFEPEK-EPD 262
           LP++  YA   G  A V+G  AL LSRA SI+ML+AY+AYL FQL THR+LFEP++ E D
Sbjct: 211 LPLMLRYAVTSGDHAIVSGDAALHLSRACSILMLIAYLAYLFFQLNTHRQLFEPQQVEDD 270

Query: 263 EYDDVV---EEAPVIGIWSAMAWLVGSTLLIALLSEYVVATIEDASETWGLSVAFLSIIL 322
           + DD+V   ++ PV+G  SAM WL   TLL ALLS YVV+TIE ASE+W LSV+F+SIIL
Sbjct: 271 DDDDLVIAQDDEPVLGFSSAMIWLALMTLLTALLSGYVVSTIEAASESWELSVSFISIIL 330

Query: 323 LPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPLCVIVAWAMGMNMDLDFN 382
           LP+VGNAAEHAGAVIFA KNK+DITLGV+LGSATQI+MFVVP+ VIVAW MG+ MDLDFN
Sbjct: 331 LPIVGNAAEHAGAVIFALKNKMDITLGVSLGSATQISMFVVPVSVIVAWTMGIPMDLDFN 390

Query: 383 ILETVSLALSIMASSSILQDGTSHYMKGLILLLFYIFISTCFFL 420
           +LET SL L+I+ ++  LQ+G SHY+KGLIL+L Y  IS CFF+
Sbjct: 391 LLETGSLFLAILVTAFTLQEGESHYLKGLILVLCYAVISVCFFV 434

BLAST of Lag0027257 vs. ExPASy Swiss-Prot
Match: Q945S5 (Vacuolar cation/proton exchanger 4 OS=Arabidopsis thaliana OX=3702 GN=CAX4 PE=1 SV=2)

HSP 1 Score: 473.4 bits (1217), Expect = 2.8e-132
Identity = 253/428 (59.11%), Postives = 332/428 (77.57%), Query Frame = 0

Query: 8   SILQNG-------AVKGSLRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQEV 67
           S+L+NG         + S R  RTV   +SS +RKRSDL L+S+VR+  +R +L NLQEV
Sbjct: 13  SLLENGGGGSDKPTAETSRRVRRTV--SASSLIRKRSDLKLISRVRWEFMRRILTNLQEV 72

Query: 68  ILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPTVG 127
           +LGT+++ILFPA+PLAV+A+ +   R WVF LSL+GL PLAER+SFLTEQI++ TGPTVG
Sbjct: 73  LLGTKLFILFPAVPLAVVAHRYDCPRAWVFALSLLGLTPLAERISFLTEQIAFHTGPTVG 132

Query: 128 GLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNKEQ 187
           GL+NATCGNATE+IIAI A+ + K+ +VK SLLGS+LSNLL VLGTSLF+GGI NL K Q
Sbjct: 133 GLMNATCGNATEMIIAILAVGQRKMRIVKLSLLGSILSNLLFVLGTSLFLGGISNLRKHQ 192

Query: 188 TYDRRQAEVNAHMLLLALLCHSLPMIFPYAGGSADVAGAGALRLSRAGSIVMLVAYVAYL 247
           ++D RQ ++N+ +L LALLC +LPMI  +   + +  G+  + LSRA S VML+AY+A+L
Sbjct: 193 SFDPRQGDMNSMLLYLALLCQTLPMIMRFTMEAEEYDGSDVVVLSRASSFVMLIAYLAFL 252

Query: 248 IFQLWTHRRLFEPEKEP-------DEYDDVVEEAPVIGIWSAMAWLVGSTLLIALLSEYV 307
           IF L++      P   P       D+  D  EE  VIG+WSA+ WL+  TLL+ALLS+Y+
Sbjct: 253 IFHLFSSHLSPPPPPLPQREDVHDDDVSDKEEEGAVIGMWSAIFWLIIMTLLVALLSDYL 312

Query: 308 VATIEDASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAM 367
           V+TI+DA+++WGLSV F+ IILLP+VGNAAEHAGAVIFA++NKLDITLG+ALGSATQIA+
Sbjct: 313 VSTIQDAADSWGLSVGFIGIILLPIVGNAAEHAGAVIFAFRNKLDITLGIALGSATQIAL 372

Query: 368 FVVPLCVIVAWAMGMNMDLDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFYIFI 422
           FVVP+ V+VAW MG+ MDL+FN+LET   ALSI+ +S +LQDGTS+YMKGL+LLL Y+ I
Sbjct: 373 FVVPVTVLVAWTMGIEMDLNFNLLETACFALSILVTSLVLQDGTSNYMKGLVLLLCYVVI 432

BLAST of Lag0027257 vs. ExPASy TrEMBL
Match: A0A6J1I673 (Vacuolar cation/proton exchanger OS=Cucurbita maxima OX=3661 GN=LOC111470279 PE=3 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 2.6e-197
Identity = 372/438 (84.93%), Postives = 399/438 (91.10%), Query Frame = 0

Query: 1   MDFEQGTSILQNGAVKGSLRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQEV 60
           MDFEQ T ILQNGAVKGSL  GRT HNMSSSSLR++SDLTLVSKVRFG LRLLLVNLQEV
Sbjct: 1   MDFEQETPILQNGAVKGSL-IGRTAHNMSSSSLRRKSDLTLVSKVRFGCLRLLLVNLQEV 60

Query: 61  ILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPTVG 120
           ILGT+IW LFPAIPLAVLA+S GFGR WVFVLSL+GLAPLAERLSFLTEQISYFTGPT+G
Sbjct: 61  ILGTKIWFLFPAIPLAVLADSLGFGRAWVFVLSLLGLAPLAERLSFLTEQISYFTGPTIG 120

Query: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNKEQ 180
           GLLNATCGNATELIIAIFALSK KIELVKYSLLGSVLSNLL+VLGTSLFI GIVNL ++Q
Sbjct: 121 GLLNATCGNATELIIAIFALSKDKIELVKYSLLGSVLSNLLVVLGTSLFISGIVNLKRQQ 180

Query: 181 TYDRRQAEVNAHMLLLALLCHSLPMIFPYAGGSADVAGAGALRLSRAGSIVMLVAYVAYL 240
           TYD+RQ EVN+HMLLLALLCH LPM+FPY  GS DV  A ALRLSRA SIVMLVAYVAYL
Sbjct: 181 TYDQRQVEVNSHMLLLALLCHLLPMLFPYVKGSTDVVVAKALRLSRASSIVMLVAYVAYL 240

Query: 241 IFQLWTHRRLFEPEKEPDEY-DDVVEEAPVIGIWSAMAWLVGSTLLIALLSEYVVATIED 300
           IFQLWTHR  FEP+KE D Y DDVV+EAP IGIWS +AWLVG T+LIALLSEYVVATIED
Sbjct: 241 IFQLWTHREFFEPDKELDVYNDDVVQEAPTIGIWSTVAWLVGVTILIALLSEYVVATIED 300

Query: 301 ASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPLC 360
           ASE+WGLSVAFLS+ILLPLVGNAAEHAGA+IFAYKNKLDITLGVALGSATQIAMFVVPLC
Sbjct: 301 ASESWGLSVAFLSMILLPLVGNAAEHAGALIFAYKNKLDITLGVALGSATQIAMFVVPLC 360

Query: 361 VIVAWAMGMNMDLDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFYIFISTCFFL 420
           VIV W MG+NMDL  N+LET SLALSIMASSSILQDG+SHYMKGLIL+LFYI I+ CFF 
Sbjct: 361 VIVGWIMGINMDLKCNVLETASLALSIMASSSILQDGSSHYMKGLILILFYIIIAICFFF 420

Query: 421 TNDEVTIINSGLSRVQLE 438
           TNDEVTI++S  SRVQL+
Sbjct: 421 TNDEVTIVHSQSSRVQLK 437

BLAST of Lag0027257 vs. ExPASy TrEMBL
Match: A0A6J1GA06 (Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111452282 PE=3 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.4e-195
Identity = 369/438 (84.25%), Postives = 399/438 (91.10%), Query Frame = 0

Query: 1   MDFEQGTSILQNGAVKGSLRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQEV 60
           MDFEQ T ILQNGAVK SL  GRT HNMSSSSLR++SDLTLVSKVRFG LRLLLVNLQEV
Sbjct: 1   MDFEQETPILQNGAVKSSL-IGRTAHNMSSSSLRRKSDLTLVSKVRFGCLRLLLVNLQEV 60

Query: 61  ILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPTVG 120
           ILGT+IW LFPAIPLAVLA+S GFGR WVFVLSL+GLAPLAERLSFLTEQISYFTGPT+G
Sbjct: 61  ILGTKIWFLFPAIPLAVLADSLGFGRAWVFVLSLLGLAPLAERLSFLTEQISYFTGPTIG 120

Query: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNKEQ 180
           GLLNATCGNATELIIAIFALSK KIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNL ++Q
Sbjct: 121 GLLNATCGNATELIIAIFALSKDKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLKRQQ 180

Query: 181 TYDRRQAEVNAHMLLLALLCHSLPMIFPYAGGSADVAGAGALRLSRAGSIVMLVAYVAYL 240
           TYD+RQAEVN+HMLLLAL+CH LPM+FPY  GS DV  A ALRLSRA SIVMLVAYVAYL
Sbjct: 181 TYDQRQAEVNSHMLLLALVCHLLPMLFPYVRGSTDVVVAEALRLSRASSIVMLVAYVAYL 240

Query: 241 IFQLWTHRRLFEPEKEPDEY-DDVVEEAPVIGIWSAMAWLVGSTLLIALLSEYVVATIED 300
           IFQLWTH   FEP+KE + Y DDVV+EAP IGIWS +AWL G T+LIALLSEYVVATIED
Sbjct: 241 IFQLWTHCEFFEPDKELEVYNDDVVQEAPTIGIWSIVAWLGGVTILIALLSEYVVATIED 300

Query: 301 ASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPLC 360
            SE+WGLSVAFLS+ILLPLVGNAAEHAGA+IFAYKNKLDITLGVALGSATQIAMFVVPLC
Sbjct: 301 VSESWGLSVAFLSMILLPLVGNAAEHAGALIFAYKNKLDITLGVALGSATQIAMFVVPLC 360

Query: 361 VIVAWAMGMNMDLDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFYIFISTCFFL 420
           VIV W MG+NMDL+FN+LET SLALSIMASSSILQDG+SHYMKGLIL+LFYI I+ CFF 
Sbjct: 361 VIVGWIMGINMDLNFNVLETASLALSIMASSSILQDGSSHYMKGLILILFYIIIAICFFF 420

Query: 421 TNDEVTIINSGLSRVQLE 438
           T+DEV I++S LSRVQL+
Sbjct: 421 TSDEVIIVHSQLSRVQLK 437

BLAST of Lag0027257 vs. ExPASy TrEMBL
Match: A0A5D3DMV0 (Vacuolar cation/proton exchanger OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00830 PE=3 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 1.8e-195
Identity = 368/439 (83.83%), Postives = 398/439 (90.66%), Query Frame = 0

Query: 1   MDFEQG-TSILQN-GAVKGSLRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQ 60
           MDFE G TSILQN G VK S R  R  HNMSSSSLRK+SDLTLVSKVRFG LRL LVNLQ
Sbjct: 1   MDFEPGTTSILQNAGGVKSSQRLARMAHNMSSSSLRKKSDLTLVSKVRFGSLRLFLVNLQ 60

Query: 61  EVILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPT 120
           EVILGTRIWILFPA+PLAVLA+SFGF RPWVFV+SLMGLAPLAERLSFLTEQISYFTGPT
Sbjct: 61  EVILGTRIWILFPAVPLAVLADSFGFARPWVFVVSLMGLAPLAERLSFLTEQISYFTGPT 120

Query: 121 VGGLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNK 180
           VGGLLNATCGN TELI+AIFAL K KI+LVKYSLLGS+LSNLLLVLGTSLF GGI+NLNK
Sbjct: 121 VGGLLNATCGNVTELIVAIFALRKEKIKLVKYSLLGSLLSNLLLVLGTSLFFGGILNLNK 180

Query: 181 EQTYDRRQAEVNAHMLLLALLCHSLPMIFPYAGGSADVAGAGALRLSRAGSIVMLVAYVA 240
           +QT+D  QAEVN+HML+LALLCHSLPM+FPYAGGS DVAG GA+RLSRA SIVMLVAYVA
Sbjct: 181 QQTFDSSQAEVNSHMLMLALLCHSLPMLFPYAGGSVDVAGVGAVRLSRACSIVMLVAYVA 240

Query: 241 YLIFQLWTHRRLFEPEKEPDEYDDVVEEAPVIGIWSAMAWLVGSTLLIALLSEYVVATIE 300
           YLIFQLW+HRRLFE  +E D YDD +EE PVIG WSAMAWLV  TLL+ALLSEYVVATIE
Sbjct: 241 YLIFQLWSHRRLFEANEELDVYDDGLEEGPVIGKWSAMAWLVVVTLLVALLSEYVVATIE 300

Query: 301 DASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL 360
           DASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQI MFVVPL
Sbjct: 301 DASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIGMFVVPL 360

Query: 361 CVIVAWAMGMNMDLDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFYIFISTCFF 420
            VIVAW MG+NMDL+FNI+ET SLALS++ASSSILQDG SHYMKGLILLLFYI I+T FF
Sbjct: 361 SVIVAWVMGINMDLNFNIIETASLALSVLASSSILQDGNSHYMKGLILLLFYIVIATYFF 420

Query: 421 LTNDEVTIINSGLSRVQLE 438
           LT+D+VT+ N GLS ++L+
Sbjct: 421 LTSDDVTVNNLGLSSIKLK 439

BLAST of Lag0027257 vs. ExPASy TrEMBL
Match: A0A1S3B6F4 (Vacuolar cation/proton exchanger OS=Cucumis melo OX=3656 GN=LOC103486299 PE=3 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 1.8e-195
Identity = 368/439 (83.83%), Postives = 398/439 (90.66%), Query Frame = 0

Query: 1   MDFEQG-TSILQN-GAVKGSLRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQ 60
           MDFE G TSILQN G VK S R  R  HNMSSSSLRK+SDLTLVSKVRFG LRL LVNLQ
Sbjct: 28  MDFEPGTTSILQNAGGVKSSQRLARMAHNMSSSSLRKKSDLTLVSKVRFGSLRLFLVNLQ 87

Query: 61  EVILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPT 120
           EVILGTRIWILFPA+PLAVLA+SFGF RPWVFV+SLMGLAPLAERLSFLTEQISYFTGPT
Sbjct: 88  EVILGTRIWILFPAVPLAVLADSFGFARPWVFVVSLMGLAPLAERLSFLTEQISYFTGPT 147

Query: 121 VGGLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNK 180
           VGGLLNATCGN TELI+AIFAL K KI+LVKYSLLGS+LSNLLLVLGTSLF GGI+NLNK
Sbjct: 148 VGGLLNATCGNVTELIVAIFALRKEKIKLVKYSLLGSLLSNLLLVLGTSLFFGGILNLNK 207

Query: 181 EQTYDRRQAEVNAHMLLLALLCHSLPMIFPYAGGSADVAGAGALRLSRAGSIVMLVAYVA 240
           +QT+D  QAEVN+HML+LALLCHSLPM+FPYAGGS DVAG GA+RLSRA SIVMLVAYVA
Sbjct: 208 QQTFDSSQAEVNSHMLMLALLCHSLPMLFPYAGGSVDVAGVGAVRLSRACSIVMLVAYVA 267

Query: 241 YLIFQLWTHRRLFEPEKEPDEYDDVVEEAPVIGIWSAMAWLVGSTLLIALLSEYVVATIE 300
           YLIFQLW+HRRLFE  +E D YDD +EE PVIG WSAMAWLV  TLL+ALLSEYVVATIE
Sbjct: 268 YLIFQLWSHRRLFEANEELDVYDDGLEEGPVIGKWSAMAWLVVVTLLVALLSEYVVATIE 327

Query: 301 DASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL 360
           DASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQI MFVVPL
Sbjct: 328 DASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIGMFVVPL 387

Query: 361 CVIVAWAMGMNMDLDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFYIFISTCFF 420
            VIVAW MG+NMDL+FNI+ET SLALS++ASSSILQDG SHYMKGLILLLFYI I+T FF
Sbjct: 388 SVIVAWVMGINMDLNFNIIETASLALSVLASSSILQDGNSHYMKGLILLLFYIVIATYFF 447

Query: 421 LTNDEVTIINSGLSRVQLE 438
           LT+D+VT+ N GLS ++L+
Sbjct: 448 LTSDDVTVNNLGLSSIKLK 466

BLAST of Lag0027257 vs. ExPASy TrEMBL
Match: A0A6J1CUP6 (Vacuolar cation/proton exchanger OS=Momordica charantia OX=3673 GN=LOC111014944 PE=3 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 5.3e-195
Identity = 366/434 (84.33%), Postives = 395/434 (91.01%), Query Frame = 0

Query: 1   MDFEQGTSILQNGAVKGSLRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQEV 60
           MD E GT IL+NG VKGSLRHGRT HN+SSS+LRKRSDL LVSKVRFG LR+ LVNLQEV
Sbjct: 1   MDLEPGTPILENGVVKGSLRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEV 60

Query: 61  ILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPTVG 120
           ILGTRI +LFPAIP A+LA SFGFGRPWVFVLSL+GLAPLAERLSFLTEQISYFTGPTVG
Sbjct: 61  ILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVG 120

Query: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNKEQ 180
           GLLNATCGNATELIIAIFALSKHKIELVKYSL+GSVLSNLLLVLG+SLF GGIVNL KEQ
Sbjct: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQ 180

Query: 181 TYDRRQAEVNAHMLLLALLCHSLPMIFPYAGGSADVAGAGALRLSRAGSIVMLVAYVAYL 240
            YDRRQAEVN+ MLLLALLC SLP++F YAG SADVAG G LRLSRAGSIVMLVAY AYL
Sbjct: 181 IYDRRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFAYL 240

Query: 241 IFQLWT-HRRLFEPEKEPDEY-DDVVEEAPVIGIWSAMAWLVGSTLLIALLSEYVVATIE 300
            FQL   HR+LFE +KEP EY DDVVEE P IG+WS +AWLV  T+++ALLSEYVVATIE
Sbjct: 241 SFQLLPHHRQLFEEDKEPYEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVATIE 300

Query: 301 DASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL 360
           DASETWGLSV FLSIILLP+VGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL
Sbjct: 301 DASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL 360

Query: 361 CVIVAWAMGMNMDLDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFYIFISTCFF 420
           CVIVAW MGMNMDLDFNIL+T SLALSIMASSSILQ+GTSHY+KGLILLLFY+ I+TCFF
Sbjct: 361 CVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLLFYVVIATCFF 420

Query: 421 LTNDEVTIINSGLS 433
           LTN+++TI+NS  S
Sbjct: 421 LTNEDITIVNSETS 434

BLAST of Lag0027257 vs. TAIR 10
Match: AT3G51860.1 (cation exchanger 3 )

HSP 1 Score: 538.9 bits (1387), Expect = 3.8e-153
Identity = 288/429 (67.13%), Postives = 352/429 (82.05%), Query Frame = 0

Query: 4   EQGTSILQNG----AVKGS---LRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVN 63
           E   +I +NG      KGS   LRHGRT HNMSSSSLRK+SDL LV KV    L+ +L N
Sbjct: 6   EPWAAIAENGNANVTAKGSSRELRHGRTAHNMSSSSLRKKSDLRLVQKVPCKTLKNILSN 65

Query: 64  LQEVILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTG 123
           LQEVILGT++ +LF AIPLA+LANS+ +GRP +F LSL+GL PLAER+SFLTEQ++++TG
Sbjct: 66  LQEVILGTKLTLLFLAIPLAILANSYNYGRPLIFGLSLIGLTPLAERVSFLTEQLAFYTG 125

Query: 124 PTVGGLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNL 183
           PTVGGLLNATCGNATELIIAI AL+ +K+ +VKYSLLGS+LSNLLLVLGTSLF GGI N+
Sbjct: 126 PTVGGLLNATCGNATELIIAILALANNKVAVVKYSLLGSILSNLLLVLGTSLFFGGIANI 185

Query: 184 NKEQTYDRRQAEVNAHMLLLALLCHSLPMIFPYAGG---SADVAGAGALRLSRAGSIVML 243
            +EQ +DR+QA+VN  +LL+ LLCH LP++  YA     S  +    +L LSR  SIVML
Sbjct: 186 RREQRFDRKQADVNFFLLLMGLLCHLLPLLLKYAATGEVSTSMINKMSLTLSRTSSIVML 245

Query: 244 VAYVAYLIFQLWTHRRLFEPEKEPDE-YDD--VVEEAPVIGIWSAMAWLVGSTLLIALLS 303
           +AY+AYLIFQLWTHR+LFE +++ D+ YDD   VEE PVIG WS  AWLVG T++IALLS
Sbjct: 246 IAYIAYLIFQLWTHRQLFEAQQDDDDAYDDEVSVEETPVIGFWSGFAWLVGMTIVIALLS 305

Query: 304 EYVVATIEDASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQ 363
           EYVV TIEDAS++WGLSV+F+SIILLP+VGNAAEHAGA+IFA+KNKLDI+LGVALGSATQ
Sbjct: 306 EYVVDTIEDASDSWGLSVSFISIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQ 365

Query: 364 IAMFVVPLCVIVAWAMGMNMDLDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFY 420
           I++FVVPL VIVAW +G+ MDL+FNILET SLAL+I+ ++  LQDGTSHYMKGL+LLL Y
Sbjct: 366 ISLFVVPLSVIVAWILGIKMDLNFNILETSSLALAIIITAFTLQDGTSHYMKGLVLLLCY 425

BLAST of Lag0027257 vs. TAIR 10
Match: AT2G38170.1 (cation exchanger 1 )

HSP 1 Score: 514.6 bits (1324), Expect = 7.7e-146
Identity = 274/423 (64.78%), Postives = 338/423 (79.91%), Query Frame = 0

Query: 8   SILQNG----AVKGS---LRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQEV 67
           S+ +NG      KGS   LR GRT HNMSSSSLRK+SDL ++ KV + GL+  L NLQEV
Sbjct: 10  SVAENGNPSITAKGSSRELRLGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFLSNLQEV 69

Query: 68  ILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPTVG 127
           ILGT++ ILFPAIP A++    G  +PW+F LSL+GL PLAER+SFLTEQ++++TGPT+G
Sbjct: 70  ILGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFYTGPTLG 129

Query: 128 GLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNKEQ 187
           GLLNATCGNATELIIAI AL+ +K+ +VKYSLLGS+LSNLLLVLGTSLF GGI N+ +EQ
Sbjct: 130 GLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIANIRREQ 189

Query: 188 TYDRRQAEVNAHMLLLALLCHSLPMIFPY---AGGSADVAGAGALRLSRAGSIVMLVAYV 247
            +DR+QA+VN  +LLL  LCH LP++  Y      SA V     L +SR  SIVML++Y+
Sbjct: 190 RFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIVMLISYI 249

Query: 248 AYLIFQLWTHRRLFEPEKEPDEYDDVVE-EAPVIGIWSAMAWLVGSTLLIALLSEYVVAT 307
           AYL+FQLWTHR+LF+ +++ DEYDD VE E  VI  WS  AWLVG TL+IALLSEYVVAT
Sbjct: 250 AYLVFQLWTHRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLSEYVVAT 309

Query: 308 IEDASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVV 367
           IE+AS+ W LSV+F+SIILLP+VGNAAEHAGAVIFA+KNKLDI+LGVALGSATQI +FVV
Sbjct: 310 IEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLDISLGVALGSATQIGLFVV 369

Query: 368 PLCVIVAWAMGMNMDLDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFYIFISTC 420
           PL +IVAW +G+NMDL+F  LET  LA+SI+ ++  LQDG+SHYMKGL+LLL Y  I+ C
Sbjct: 370 PLTIIVAWILGINMDLNFGPLETGCLAVSIIITAFTLQDGSSHYMKGLVLLLCYFIIAIC 429

BLAST of Lag0027257 vs. TAIR 10
Match: AT2G38170.3 (cation exchanger 1 )

HSP 1 Score: 514.6 bits (1324), Expect = 7.7e-146
Identity = 274/423 (64.78%), Postives = 338/423 (79.91%), Query Frame = 0

Query: 8   SILQNG----AVKGS---LRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQEV 67
           S+ +NG      KGS   LR GRT HNMSSSSLRK+SDL ++ KV + GL+  L NLQEV
Sbjct: 10  SVAENGNPSITAKGSSRELRLGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFLSNLQEV 69

Query: 68  ILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPTVG 127
           ILGT++ ILFPAIP A++    G  +PW+F LSL+GL PLAER+SFLTEQ++++TGPT+G
Sbjct: 70  ILGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFYTGPTLG 129

Query: 128 GLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNKEQ 187
           GLLNATCGNATELIIAI AL+ +K+ +VKYSLLGS+LSNLLLVLGTSLF GGI N+ +EQ
Sbjct: 130 GLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIANIRREQ 189

Query: 188 TYDRRQAEVNAHMLLLALLCHSLPMIFPY---AGGSADVAGAGALRLSRAGSIVMLVAYV 247
            +DR+QA+VN  +LLL  LCH LP++  Y      SA V     L +SR  SIVML++Y+
Sbjct: 190 RFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIVMLISYI 249

Query: 248 AYLIFQLWTHRRLFEPEKEPDEYDDVVE-EAPVIGIWSAMAWLVGSTLLIALLSEYVVAT 307
           AYL+FQLWTHR+LF+ +++ DEYDD VE E  VI  WS  AWLVG TL+IALLSEYVVAT
Sbjct: 250 AYLVFQLWTHRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLSEYVVAT 309

Query: 308 IEDASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVV 367
           IE+AS+ W LSV+F+SIILLP+VGNAAEHAGAVIFA+KNKLDI+LGVALGSATQI +FVV
Sbjct: 310 IEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLDISLGVALGSATQIGLFVV 369

Query: 368 PLCVIVAWAMGMNMDLDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFYIFISTC 420
           PL +IVAW +G+NMDL+F  LET  LA+SI+ ++  LQDG+SHYMKGL+LLL Y  I+ C
Sbjct: 370 PLTIIVAWILGINMDLNFGPLETGCLAVSIIITAFTLQDGSSHYMKGLVLLLCYFIIAIC 429

BLAST of Lag0027257 vs. TAIR 10
Match: AT5G01490.1 (cation exchanger 4 )

HSP 1 Score: 475.3 bits (1222), Expect = 5.2e-134
Identity = 255/436 (58.49%), Postives = 335/436 (76.83%), Query Frame = 0

Query: 8   SILQNG-------AVKGSLRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQEV 67
           S+L+NG         + S R  RTV   +SS +RKRSDL L+S+VR+  +R +L NLQEV
Sbjct: 13  SLLENGGGGSDKPTAETSRRVRRTV--SASSLIRKRSDLKLISRVRWEFMRRILTNLQEV 72

Query: 68  ILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPTVG 127
           +LGT+++ILFPA+PLAV+A+ +   R WVF LSL+GL PLAER+SFLTEQI++ TGPTVG
Sbjct: 73  LLGTKLFILFPAVPLAVVAHRYDCPRAWVFALSLLGLTPLAERISFLTEQIAFHTGPTVG 132

Query: 128 GLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNKEQ 187
           GL+NATCGNATE+IIAI A+ + K+ +VK SLLGS+LSNLL VLGTSLF+GGI NL K Q
Sbjct: 133 GLMNATCGNATEMIIAILAVGQRKMRIVKLSLLGSILSNLLFVLGTSLFLGGISNLRKHQ 192

Query: 188 TYDRRQAEVNAHMLLLALLCHSLPMIFPYAGGSADVAGAGALRLSRAGSIVMLVAYVAYL 247
           ++D RQ ++N+ +L LALLC +LPMI  +   + +  G+  + LSRA S VML+AY+A+L
Sbjct: 193 SFDPRQGDMNSMLLYLALLCQTLPMIMRFTMEAEEYDGSDVVVLSRASSFVMLIAYLAFL 252

Query: 248 IFQLWTHRRLFEPEKEP-------DEYDDVVEEAPVIGIWSAMAWLVGSTLLIALLSEYV 307
           IF L++      P   P       D+  D  EE  VIG+WSA+ WL+  TLL+ALLS+Y+
Sbjct: 253 IFHLFSSHLSPPPPPLPQREDVHDDDVSDKEEEGAVIGMWSAIFWLIIMTLLVALLSDYL 312

Query: 308 VATIEDASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAM 367
           V+TI+DA+++WGLSV F+ IILLP+VGNAAEHAGAVIFA++NKLDITLG+ALGSATQIA+
Sbjct: 313 VSTIQDAADSWGLSVGFIGIILLPIVGNAAEHAGAVIFAFRNKLDITLGIALGSATQIAL 372

Query: 368 FVVPLCVIVAWAMGMNMDLDFNILETVSLALSIMASSSILQDGTSHYMKGLILLLFYIFI 427
           FVVP+ V+VAW MG+ MDL+FN+LET   ALSI+ +S +LQDGTS+YMKGL+LLL Y+ I
Sbjct: 373 FVVPVTVLVAWTMGIEMDLNFNLLETACFALSILVTSLVLQDGTSNYMKGLVLLLCYVVI 432

Query: 428 STCFFLTNDEVTIINS 430
           + CFF++N   T  N+
Sbjct: 433 AACFFVSNSPSTETNT 446

BLAST of Lag0027257 vs. TAIR 10
Match: AT2G38170.2 (cation exchanger 1 )

HSP 1 Score: 414.8 bits (1065), Expect = 8.3e-116
Identity = 220/341 (64.52%), Postives = 270/341 (79.18%), Query Frame = 0

Query: 8   SILQNG----AVKGS---LRHGRTVHNMSSSSLRKRSDLTLVSKVRFGGLRLLLVNLQEV 67
           S+ +NG      KGS   LR GRT HNMSSSSLRK+SDL ++ KV + GL+  L NLQEV
Sbjct: 10  SVAENGNPSITAKGSSRELRLGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFLSNLQEV 69

Query: 68  ILGTRIWILFPAIPLAVLANSFGFGRPWVFVLSLMGLAPLAERLSFLTEQISYFTGPTVG 127
           ILGT++ ILFPAIP A++    G  +PW+F LSL+GL PLAER+SFLTEQ++++TGPT+G
Sbjct: 70  ILGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFYTGPTLG 129

Query: 128 GLLNATCGNATELIIAIFALSKHKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLNKEQ 187
           GLLNATCGNATELIIAI AL+ +K+ +VKYSLLGS+LSNLLLVLGTSLF GGI N+ +EQ
Sbjct: 130 GLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIANIRREQ 189

Query: 188 TYDRRQAEVNAHMLLLALLCHSLPMIFPY---AGGSADVAGAGALRLSRAGSIVMLVAYV 247
            +DR+QA+VN  +LLL  LCH LP++  Y      SA V     L +SR  SIVML++Y+
Sbjct: 190 RFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIVMLISYI 249

Query: 248 AYLIFQLWTHRRLFEPEKEPDEYDDVVE-EAPVIGIWSAMAWLVGSTLLIALLSEYVVAT 307
           AYL+FQLWTHR+LF+ +++ DEYDD VE E  VI  WS  AWLVG TL+IALLSEYVVAT
Sbjct: 250 AYLVFQLWTHRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLSEYVVAT 309

Query: 308 IEDASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKL 338
           IE+AS+ W LSV+F+SIILLP+VGNAAEHAGAVIFA+KNKL
Sbjct: 310 IEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKL 350