Lag0027211 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0027211 vs. NCBI nr
Match: XP_038906230.1 (fructokinase-like 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 845.1 bits (2182), Expect = 2.9e-241
Identity = 437/513 (85.19%), Postives = 454/513 (88.50%), Query Frame = 0

Query: 1   MASFQL------LPHLQFSCFLPFYPSNCRAKAL----------------------IRAS 60
           MASFQL      LPH+QFSCFLPFY SN RAK L                       RAS
Sbjct: 1   MASFQLLPSTLSLPHVQFSCFLPFYSSNYRAKTLDLSLIFKSHHLQSSSQSLKQNTPRAS 60

Query: 61  -NGDGNGVVETPNPTRRGRKKGTKSSSSSTTTQKTAKRTKK-------TERNSFHQISED 120
            NGDGNGVVETP+PTRRGRKKGT S SSS+T +KT KR+KK       TE N F   S  
Sbjct: 61  VNGDGNGVVETPSPTRRGRKKGTTSPSSSSTQKKT-KRSKKPQITDETTEGNPFDHAS-- 120

Query: 121 EDEPIHLIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHPDKYSEWKMLQW 180
           EDEPI  IA+NYDDGMD PYEDPPLICCFGAAQKEFVPS+RVHDNQMHPDKYSEWKMLQW
Sbjct: 121 EDEPIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQW 180

Query: 181 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 240
           DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDE+VLMMNKEKVQTRAVKFDL
Sbjct: 181 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDEVVLMMNKEKVQTRAVKFDL 240

Query: 241 NSRTACTYMKVKFEDGKLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAME 300
           NSRTACTYMK+KFEDG+LKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEAL+STAM+
Sbjct: 241 NSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALLSTAMK 300

Query: 301 STLFKAIQLSKKFGGLIFFDLNLPLPLWRSRDETQEYIKKAWNEADIIEVSRQELEFLLD 360
           STLFKAIQLSKKFGGLIFFDLNLPLPLW+SRDET+EYIKKAWNEADIIEVSRQELEFLLD
Sbjct: 301 STLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEFLLD 360

Query: 361 EEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISSLWHDRLKLLFVTDGTLRIHYYS 420
           EEYYEKKRNYRPQYYAQTIEQTKNRRD+YHYTPEEIS LWHDRLKLLFVTDGTLRIHYYS
Sbjct: 361 EEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRIHYYS 420

Query: 421 PSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAVAA 478
           PSFHGV IGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPE+  NQD LERQLRFA+AA
Sbjct: 421 PSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEILVNQDALERQLRFAIAA 480

BLAST of Lag0027211 vs. NCBI nr
Match: XP_022145754.1 (fructokinase-like 1, chloroplastic [Momordica charantia])

HSP 1 Score: 831.2 bits (2146), Expect = 4.4e-237
Identity = 438/520 (84.23%), Postives = 451/520 (86.73%), Query Frame = 0

Query: 1   MASFQL------LPHLQFSCFLPFYPSNCRAKAL-------------------------- 60
           MASFQL      LPHL FS FL   PS+CR +                            
Sbjct: 1   MASFQLLPATISLPHLHFSRFLS-NPSHCRYQTTELLNPLDSFLIFNSRYVQNSSKSPRL 60

Query: 61  --IRASNG-DGNGVVETPNPTRRGRKKG-TKSSSSSTTTQKTAKRTKK-------TERNS 120
              RAS G DGNGVVET +PTRRGRKKG TKSSSSSTTTQK  KR+KK       TE N 
Sbjct: 61  NPARASVGDDGNGVVETASPTRRGRKKGMTKSSSSSTTTQKKTKRSKKTLVEDETTEGNP 120

Query: 121 FHQISEDEDEPIHLIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHPDKYS 180
           F Q+S  EDEPI  IAE YDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMH DKYS
Sbjct: 121 FEQVS--EDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHADKYS 180

Query: 181 EWKMLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQT 240
           EWKMLQWDPPEFARAPGG+PSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQT
Sbjct: 181 EWKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQT 240

Query: 241 RAVKFDLNSRTACTYMKVKFEDGKLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEA 300
           RAVKFDLNSRTACTYMK+KFEDGKLKMETVKE AEDSL+SSELNL+VLKEARIFHFNSEA
Sbjct: 241 RAVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVLKEARIFHFNSEA 300

Query: 301 LMSTAMESTLFKAIQLSKKFGGLIFFDLNLPLPLWRSRDETQEYIKKAWNEADIIEVSRQ 360
           LMSTAMESTLFKAIQLSKKFGGLIFFDLNL LPLWRSRDET+EYIKKAWNEADIIEVSRQ
Sbjct: 301 LMSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKAWNEADIIEVSRQ 360

Query: 361 ELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISSLWHDRLKLLFVTDGT 420
           ELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEIS LWHDRL+LLFVTDGT
Sbjct: 361 ELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWHDRLRLLFVTDGT 420

Query: 421 LRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQ 478
           +RIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQD LERQ
Sbjct: 421 MRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDXLERQ 480

BLAST of Lag0027211 vs. NCBI nr
Match: XP_016899692.1 (PREDICTED: fructokinase-like 1, chloroplastic [Cucumis melo] >KAA0044141.1 fructokinase-like 1 [Cucumis melo var. makuwa] >TYK24996.1 fructokinase-like 1 [Cucumis melo var. makuwa])

HSP 1 Score: 830.5 bits (2144), Expect = 7.4e-237
Identity = 428/513 (83.43%), Postives = 447/513 (87.13%), Query Frame = 0

Query: 1   MASFQL------LPHLQFSCFLPFYPSNCRAKAL----------------------IRAS 60
           MASF L      LPH+QFSCFLPFY S  RAK +                       RAS
Sbjct: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSTYRAKPIDLSLIFNLRHFQSSSKTLKLNPPRAS 60

Query: 61  -NGDGNGVVETPNPTRRGRKKGTKSSSSSTTTQKTAKRTKK-------TERNSFHQISED 120
            NG+GNGVVETP+PTRRGRKKGT S SSST+ QK  KR+KK       T  N F  ISED
Sbjct: 61  TNGEGNGVVETPSPTRRGRKKGTASPSSSTSAQKKTKRSKKPQITDKTTVANPFDHISED 120

Query: 121 EDEPIHLIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHPDKYSEWKMLQW 180
           E   I  IA+NYDDGMD PYEDPPLICCFGAAQKEFVPS+RVHDNQM  DKYSEWKMLQW
Sbjct: 121 ES--IDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMDADKYSEWKMLQW 180

Query: 181 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 240
           DPPEFARAPGGSPSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL
Sbjct: 181 DPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 240

Query: 241 NSRTACTYMKVKFEDGKLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAME 300
           NS+TACTYMK+KFEDGKLKMETVKEPAEDSL+SSELNLAVLKEARIFHFNSEAL+ST +E
Sbjct: 241 NSKTACTYMKIKFEDGKLKMETVKEPAEDSLLSSELNLAVLKEARIFHFNSEALLSTVIE 300

Query: 301 STLFKAIQLSKKFGGLIFFDLNLPLPLWRSRDETQEYIKKAWNEADIIEVSRQELEFLLD 360
            TLFKAIQLSKKFGGLIFFDLNLPLPLW+SR+ET+EYIKKAWNEADIIEVSRQELEFLLD
Sbjct: 301 PTLFKAIQLSKKFGGLIFFDLNLPLPLWKSREETREYIKKAWNEADIIEVSRQELEFLLD 360

Query: 361 EEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISSLWHDRLKLLFVTDGTLRIHYYS 420
           EEYYEKKRNYRPQYYAQTIEQTKNRRD+YHYTPEEIS LWHD LKLLFVTDGTLRIHYYS
Sbjct: 361 EEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDHLKLLFVTDGTLRIHYYS 420

Query: 421 PSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAVAA 478
           PSFHGV IGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM ENQDVLERQLRFA+AA
Sbjct: 421 PSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAA 480

BLAST of Lag0027211 vs. NCBI nr
Match: XP_031737955.1 (fructokinase-like 1, chloroplastic [Cucumis sativus] >KGN58805.1 hypothetical protein Csa_000812 [Cucumis sativus])

HSP 1 Score: 828.9 bits (2140), Expect = 2.2e-236
Identity = 427/513 (83.24%), Postives = 447/513 (87.13%), Query Frame = 0

Query: 1   MASFQL------LPHLQFSCFLPFYPSNCRAKAL----------------------IRAS 60
           MASF L      LPH+QFSCFLPFY SN RAK +                       RAS
Sbjct: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSNYRAKPIDLSLIFNFHHSQSLSKTLKLNPPRAS 60

Query: 61  -NGDGNGVVETPNPTRRGRKKGTKSSSSSTTTQKTAKRTKK-------TERNSFHQISED 120
            N +GNGVVETP PTRRGRKKGT S SSST+ QK  KR+K+       T  N F  ISED
Sbjct: 61  ANDEGNGVVETPGPTRRGRKKGTASPSSSTSAQKKTKRSKEPQITDRITVANPFVHISED 120

Query: 121 EDEPIHLIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHPDKYSEWKMLQW 180
           E   I  IA+NYDDGMD PYEDPPLICCFGAAQKEFVPS+RVHDNQMH DKYSEWKMLQW
Sbjct: 121 ES--IDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQW 180

Query: 181 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 240
           DPPEFARAPGGSPSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL
Sbjct: 181 DPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 240

Query: 241 NSRTACTYMKVKFEDGKLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAME 300
           NS+TACTYMK+KFEDGKLK ETVKEPAEDSL+SSELNLAVLKEARIFHFNSE+L+STA+E
Sbjct: 241 NSKTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAIE 300

Query: 301 STLFKAIQLSKKFGGLIFFDLNLPLPLWRSRDETQEYIKKAWNEADIIEVSRQELEFLLD 360
            TLFKAIQLSKKFGGLIFFDLNLPLPLW+SRDET+EYIKKAW EADIIEVSRQELEFLLD
Sbjct: 301 PTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLLD 360

Query: 361 EEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISSLWHDRLKLLFVTDGTLRIHYYS 420
           EEYYEKKRNYRPQYYAQTIEQTKNRRD+YHYTPEE+S LWHDRLKLLFVTDGTLRIHYYS
Sbjct: 361 EEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYYS 420

Query: 421 PSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAVAA 478
           PSFHGV IGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM ENQDVLERQLRFA+AA
Sbjct: 421 PSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAA 480

BLAST of Lag0027211 vs. NCBI nr
Match: KAG7027918.1 (Fructokinase-like 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 828.9 bits (2140), Expect = 2.2e-236
Identity = 424/502 (84.46%), Postives = 443/502 (88.25%), Query Frame = 0

Query: 1   MASFQL------LPHLQFSCFLPFYPSNCRAKALI------------------RASNGDG 60
           MASFQL      LPHLQFS FL FYPS  R K LI                   ASN DG
Sbjct: 1   MASFQLLPATFSLPHLQFSRFLHFYPSIFREKTLIFNSRYLQSSCKSLKLSPPGASNADG 60

Query: 61  NGVVETPNPTRRGRKKGTKSSSSST-TTQKTAKRTKKTERNSFHQISEDEDEPIHLIAEN 120
           N    TP PTRRGRKKGTKS SSST  ++KT    + T+ N F Q+S DEDE I  IAEN
Sbjct: 61  NEDTGTPTPTRRGRKKGTKSPSSSTKRSRKTLIEDEPTQGNLFDQMSVDEDEAIDSIAEN 120

Query: 121 YDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHPDKYSEWKMLQWDPPEFARAPGG 180
           YDDGMDFPYEDPPLICCFGAAQKEFVPS+RVHDNQMHPDKYSEWKMLQWDPPEFARAPGG
Sbjct: 121 YDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPPEFARAPGG 180

Query: 181 SPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKV 240
           SPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR VKFDLNSRTACTYMK+
Sbjct: 181 SPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRGVKFDLNSRTACTYMKI 240

Query: 241 KFEDGKLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAMESTLFKAIQLSK 300
           KF+DGKLKMETVKE AEDSLVSSELNLAVLKEARIFHFNSEAL+ST ME+TLF AIQLSK
Sbjct: 241 KFDDGKLKMETVKESAEDSLVSSELNLAVLKEARIFHFNSEALLSTTMEATLFNAIQLSK 300

Query: 301 KFGGLIFFDLNLPLPLWRSRDETQEYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYR 360
           KFGGLIFFDLNLPLPLWRSRDET++YI+KAWN+ADIIEVSRQELEFLLDEEYYEKKRNYR
Sbjct: 301 KFGGLIFFDLNLPLPLWRSRDETRKYIEKAWNQADIIEVSRQELEFLLDEEYYEKKRNYR 360

Query: 361 PQYYAQTIEQTKNRRDYYHYTPEEISSLWHDRLKLLFVTDGTLRIHYYSPSFHGVVIGTE 420
           PQYYAQTIEQTKNRRD+YHY+PEEIS LWHD LKLL +TDGTLRIHYYSPSFHGVVIGTE
Sbjct: 361 PQYYAQTIEQTKNRRDHYHYSPEEISPLWHDHLKLLLITDGTLRIHYYSPSFHGVVIGTE 420

Query: 421 DVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAVAAGIISQWTIGAV 478
           DVLITPFTCDRTGSGDA+VAGIMRKLTTFPEMFENQDVLERQLRFA+AAGIISQWTIGAV
Sbjct: 421 DVLITPFTCDRTGSGDALVAGIMRKLTTFPEMFENQDVLERQLRFAIAAGIISQWTIGAV 480

BLAST of Lag0027211 vs. ExPASy Swiss-Prot
Match: Q9M394 (Fructokinase-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN1 PE=1 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 2.5e-195
Identity = 340/479 (70.98%), Postives = 398/479 (83.09%), Query Frame = 0

Query: 1   MASFQLLPHLQFSCFLPFYPSNCRAKALIRASNGDGNGVVETPNPTRRGRKKGTKSSSSS 60
           MAS  + PHL        + S+   + ++   +   +    TP  +  G   G  + +++
Sbjct: 1   MASLLIFPHLH------HFDSSLDRREVLVVRHSQASRRFLTPKASING--SGITNGAAA 60

Query: 61  TTTQKTAKRTKKTERNS--FHQISEDEDEPIHLIAENYDDGMDFPYEDPPLICCFGAAQK 120
            TT K +++ +K ++ S    + + + D  ++    +YDDG++FPY+DPPL+CCFGA QK
Sbjct: 61  ETTSKPSRKGRKKKQTSTVIEKDNTETDPELNPELADYDDGIEFPYDDPPLVCCFGAVQK 120

Query: 121 EFVPSVRVHDNQMHPDKYSEWKMLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVG 180
           EFVP VRVHDN MHPD YS+WKMLQWDPPEF RAPGG PSNVAISHVRLGGRAAFMGKVG
Sbjct: 121 EFVPVVRVHDNPMHPDMYSQWKMLQWDPPEFGRAPGGPPSNVAISHVRLGGRAAFMGKVG 180

Query: 181 KDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKVKFEDGKLKMETVKEPAEDSLVSS 240
           +DDFGDELVLMMN+E+VQTRAVKFD NS+TACT +K+KF+DGK+  ETVKEP EDSL +S
Sbjct: 181 EDDFGDELVLMMNQERVQTRAVKFDENSKTACTRVKIKFKDGKMMAETVKEPPEDSLFAS 240

Query: 241 ELNLAVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLPLPLWRSRDET 300
           ELNLAVLKEARIFHFNSE L S  M+STLF AIQ SKKFGGLIFFDLNLPLPLWRSR+ET
Sbjct: 241 ELNLAVLKEARIFHFNSEVLTSPTMQSTLFTAIQWSKKFGGLIFFDLNLPLPLWRSRNET 300

Query: 301 QEYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPE 360
           ++ IKKAWNEA+IIEVS+QELEFLLDE+YYE++RNY PQY+A+  +QTKNRRDYYHYTPE
Sbjct: 301 RKLIKKAWNEANIIEVSQQELEFLLDEDYYERRRNYTPQYFAEDFDQTKNRRDYYHYTPE 360

Query: 361 EISSLWHDRLKLLFVTDGTLRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIM 420
           EI SLWHD+LKLL VTDGTLR+HYY+P+F GVVIGTEDVLITPFTCDRTGSGDAVVAGIM
Sbjct: 361 EIKSLWHDKLKLLVVTDGTLRLHYYTPTFDGVVIGTEDVLITPFTCDRTGSGDAVVAGIM 420

Query: 421 RKLTTFPEMFENQDVLERQLRFAVAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 478
           RKLTT PEMFE+QDV+ERQLRFAVAAGII+QWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 421 RKLTTCPEMFEDQDVMERQLRFAVAAGIIAQWTIGAVRGFPTESATQNLKEQVYVPSMW 471

BLAST of Lag0027211 vs. ExPASy Swiss-Prot
Match: F4I0K2 (Fructokinase-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN2 PE=1 SV=2)

HSP 1 Score: 323.9 bits (829), Expect = 3.0e-87
Identity = 181/441 (41.04%), Postives = 263/441 (59.64%), Query Frame = 0

Query: 20  PSNCRAKALIRASNGDGNGVVETPNPTRRGRKKGTKSSSSSTTTQKTAKRTKKTERNSFH 79
           P   R +    A++ D   V       R+   K  K       T   A+ +   E  +  
Sbjct: 117 PKKTRTRKKAAAASSDVEEVKTEKKVRRKRTVKKDKDVEDDLATIMDAEVSDVEEALAVE 176

Query: 80  QI-SEDEDEPIHLIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHPDKYSE 139
              +E E+E I L     +D +   Y  PPL+CCFG+AQ  FVPS R  +  +  + +  
Sbjct: 177 STDTESEEEEIDLSKHEGED-ISHTYGWPPLVCCFGSAQHAFVPSGRPANRLLDYELHER 236

Query: 140 WKMLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR 199
            +  +W P ++ RAPGG    VAI+   LGG+ AFMGK+G DD+G  ++  +N  KVQTR
Sbjct: 237 MRDAKWAPEKYIRAPGGCAGGVAIALASLGGKVAFMGKLGADDYGQAMLYYLNVCKVQTR 296

Query: 200 AVKFDLNSRTACTYMKVKFEDGKLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEAL 259
           +VK D    TAC+ MK+  + G+LK   +K  AEDSL  SE+N+ VLKEA++F+F++ +L
Sbjct: 297 SVKIDGKRVTACSTMKIS-KRGRLKSTCIKPCAEDSLSKSEINVDVLKEAKMFYFSTHSL 356

Query: 260 MSTAMESTLFKAIQLSKKFGGLIFFDLNLPLPLWRSRDETQEYIKKAWNEADIIEVSRQE 319
           +   M ST  +AI++SK+ G +IF+DLNLPLPLW S +ET+ +I++ WN AD+IE+++QE
Sbjct: 357 LDKKMMSTTIQAIKISKQLGNVIFYDLNLPLPLWHSSEETKSFIQEVWNLADVIEITKQE 416

Query: 320 LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISSLWHDRLKLLFVTDGTL 379
           LEFL   E  E       ++  +  + +K    + HY PE +  LWH+ LK+LFVT+GT 
Sbjct: 417 LEFLCGIEPTE-------EFDTENNDISK----FVHYPPETVEQLWHENLKVLFVTNGTS 476

Query: 380 RIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQL 439
           +IHYY+   +G V G EDV ITPFT D + SGD +VAG++R LT  P++  N+  LER  
Sbjct: 477 KIHYYTKEHNGAVSGMEDVPITPFTRDMSASGDGIVAGLIRMLTVQPDLMNNKGYLERTA 536

Query: 440 RFAVAAGIISQWTIGAVRGFP 460
           R+A+  GII QW +   RG+P
Sbjct: 537 RYAIECGIIDQWLLAQTRGYP 544

BLAST of Lag0027211 vs. ExPASy Swiss-Prot
Match: Q42896 (Fructokinase-2 OS=Solanum lycopersicum OX=4081 GN=FRK2 PE=2 SV=2)

HSP 1 Score: 196.8 bits (499), Expect = 5.5e-49
Identity = 123/360 (34.17%), Postives = 188/360 (52.22%), Query Frame = 0

Query: 109 LICCFGAAQKEFVPSVRVHDNQMHPDKYSEWKMLQWDPPEFARAPGGSPSNVAISHVRLG 168
           LI  FG    +FVP+V                +   + P F +APGG+P+NVAI+  RLG
Sbjct: 11  LIVSFGEMLIDFVPTVS--------------GVSLAEAPGFLKAPGGAPANVAIAVTRLG 70

Query: 169 GRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKVKFEDGKLKMETVK 228
           G++AF+GK+G D+FG  L  ++    VQ   + FD  +RTA  ++ ++  DG+ +    +
Sbjct: 71  GKSAFVGKLGDDEFGHMLAGILKTNGVQAEGINFDKGARTALAFVTLR-ADGEREFMFYR 130

Query: 229 EPAEDSLVS-SELNLAVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNL 288
            P+ D L++ +ELNL +++ A++FH+ S +L+     +   KA++++K+ G L+ +D NL
Sbjct: 131 NPSADMLLTPAELNLDLIRSAKVFHYGSISLIVEPCRAAHMKAMEVAKEAGALLSYDPNL 190

Query: 289 PLPLWRSRDETQEYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTK 348
            LPLW S +E ++ IK  W+ AD+I+VS  ELEFL                         
Sbjct: 191 RLPLWPSAEEAKKQIKSIWDSADVIKVSDVELEFLTG----------------------S 250

Query: 349 NRRDYYHYTPEEISSLWHDRLKLLFVTDGTLRIHYYSPSFHGVVIGTEDVLITPFTCDRT 408
           N+ D      E   SLWH  LKLL VT G    +YY+  FHG V G         T D T
Sbjct: 251 NKID-----DESAMSLWHPNLKLLLVTLGEKGCNYYTKKFHGTVGG-----FHVKTVDTT 310

Query: 409 GSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAVAAGIISQWTIGAVRGFPTESATQNL 468
           G+GD+ V  ++ K+     + E++  L+  LRF+ A G I+    GA+   PT S    L
Sbjct: 311 GAGDSFVGALLTKIVDDQTILEDEARLKEVLRFSCACGAITTTKKGAIPALPTASEALTL 323

BLAST of Lag0027211 vs. ExPASy Swiss-Prot
Match: Q7XJ81 (Fructokinase-2 OS=Solanum habrochaites OX=62890 GN=FRK2 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.1e-48
Identity = 123/360 (34.17%), Postives = 187/360 (51.94%), Query Frame = 0

Query: 109 LICCFGAAQKEFVPSVRVHDNQMHPDKYSEWKMLQWDPPEFARAPGGSPSNVAISHVRLG 168
           LI  FG    +FVP+V                +   + P F +APGG+P+NVAI+  RLG
Sbjct: 11  LIVSFGEMLIDFVPTVS--------------GVSLAEAPGFLKAPGGAPANVAIAVTRLG 70

Query: 169 GRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKVKFEDGKLKMETVK 228
           GR+AF+GK+G D+FG  L  ++    VQ   + FD  +RTA  ++ ++  DG+ +    +
Sbjct: 71  GRSAFVGKLGDDEFGHMLAGILKTNGVQADGINFDKGARTALAFVTLR-ADGEREFMFYR 130

Query: 229 EPAEDSLVS-SELNLAVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNL 288
            P+ D L++ +ELNL +++ A++FH+ S +L+     +   KA++++K+ G L+ +D NL
Sbjct: 131 NPSADMLLTPAELNLDLIRSAKVFHYGSISLIVEPCRAAHMKAMEVAKEAGALLSYDPNL 190

Query: 289 PLPLWRSRDETQEYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTK 348
            LPLW S +E ++ IK  W+ AD+I+VS  ELEFL                         
Sbjct: 191 RLPLWPSAEEAKKQIKSIWDSADVIKVSDVELEFLTG----------------------S 250

Query: 349 NRRDYYHYTPEEISSLWHDRLKLLFVTDGTLRIHYYSPSFHGVVIGTEDVLITPFTCDRT 408
           N+ D      E   SLWH  LKLL VT G    +YY+  FHG V G         T D T
Sbjct: 251 NKID-----DESAMSLWHPNLKLLLVTLGEKGCNYYTKKFHGTVGG-----FHVKTVDTT 310

Query: 409 GSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAVAAGIISQWTIGAVRGFPTESATQNL 468
           G+GD+ V  ++ K+     +  ++  L+  LRF+ A G I+    GA+   PT S    L
Sbjct: 311 GAGDSFVGALLTKIVDDQTILADEARLKEVLRFSCACGAITTTKKGAIPALPTASEALTL 323

BLAST of Lag0027211 vs. ExPASy Swiss-Prot
Match: Q9M1B9 (Probable fructokinase-4 OS=Arabidopsis thaliana OX=3702 GN=At3g59480 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.1e-48
Identity = 125/363 (34.44%), Postives = 186/363 (51.24%), Query Frame = 0

Query: 109 LICCFGAAQKEFVPSVRVHDNQMHPDKYSEWKMLQWDPPEFARAPGGSPSNVAISHVRLG 168
           LI  FG    +FVP+V                +   D P F +APGG+P+NVAI+  RLG
Sbjct: 10  LIVSFGEMLIDFVPTVS--------------GVSLADAPGFIKAPGGAPANVAIAISRLG 69

Query: 169 GRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKVKFEDGKLKMETVK 228
           GRAAF+GK+G D+FG  L  ++ +  V    + FD  +RTA  ++ ++  DG+ +    +
Sbjct: 70  GRAAFVGKLGDDEFGHMLAGILKQNGVSAEGINFDTGARTALAFVTLR-SDGEREFMFYR 129

Query: 229 EPAEDSLV-SSELNLAVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNL 288
            P+ D L+   ELNL V++ A++FH+ S +L+     S   KA++++K+ G L+ +D NL
Sbjct: 130 NPSADMLLRPDELNLDVIRSAKVFHYGSISLIVEPCRSAHLKAMEVAKEAGALLSYDPNL 189

Query: 289 PLPLWRSRDETQEYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTK 348
            LPLW S++E Q+ I   W++A++I+VS +EL FL   +  +                  
Sbjct: 190 RLPLWPSKEEAQKQILSIWDKAEVIKVSDEELMFLTGSDKVD------------------ 249

Query: 349 NRRDYYHYTPEEISSLWHDRLKLLFVTDGTLRIHYYSPSFHGVVIGTEDVLITPF---TC 408
                     E   SLWH  LKLL VT G     YY+ SF G V         PF     
Sbjct: 250 ---------DETALSLWHSNLKLLLVTLGEKGCRYYTKSFRGSV--------DPFHVDAV 309

Query: 409 DRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAVAAGIISQWTIGAVRGFPTESAT 468
           D TG+GD+ V  ++ K+     + E++  L   LR A A G I+    GA+   PTES  
Sbjct: 310 DTTGAGDSFVGALLCKIVDDRAVLEDEARLREVLRLANACGAITTTKKGAIPALPTESEV 322

BLAST of Lag0027211 vs. ExPASy TrEMBL
Match: A0A6J1CW73 (fructokinase-like 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015134 PE=4 SV=1)

HSP 1 Score: 831.2 bits (2146), Expect = 2.1e-237
Identity = 438/520 (84.23%), Postives = 451/520 (86.73%), Query Frame = 0

Query: 1   MASFQL------LPHLQFSCFLPFYPSNCRAKAL-------------------------- 60
           MASFQL      LPHL FS FL   PS+CR +                            
Sbjct: 1   MASFQLLPATISLPHLHFSRFLS-NPSHCRYQTTELLNPLDSFLIFNSRYVQNSSKSPRL 60

Query: 61  --IRASNG-DGNGVVETPNPTRRGRKKG-TKSSSSSTTTQKTAKRTKK-------TERNS 120
              RAS G DGNGVVET +PTRRGRKKG TKSSSSSTTTQK  KR+KK       TE N 
Sbjct: 61  NPARASVGDDGNGVVETASPTRRGRKKGMTKSSSSSTTTQKKTKRSKKTLVEDETTEGNP 120

Query: 121 FHQISEDEDEPIHLIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHPDKYS 180
           F Q+S  EDEPI  IAE YDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMH DKYS
Sbjct: 121 FEQVS--EDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHADKYS 180

Query: 181 EWKMLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQT 240
           EWKMLQWDPPEFARAPGG+PSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQT
Sbjct: 181 EWKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQT 240

Query: 241 RAVKFDLNSRTACTYMKVKFEDGKLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEA 300
           RAVKFDLNSRTACTYMK+KFEDGKLKMETVKE AEDSL+SSELNL+VLKEARIFHFNSEA
Sbjct: 241 RAVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVLKEARIFHFNSEA 300

Query: 301 LMSTAMESTLFKAIQLSKKFGGLIFFDLNLPLPLWRSRDETQEYIKKAWNEADIIEVSRQ 360
           LMSTAMESTLFKAIQLSKKFGGLIFFDLNL LPLWRSRDET+EYIKKAWNEADIIEVSRQ
Sbjct: 301 LMSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKAWNEADIIEVSRQ 360

Query: 361 ELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISSLWHDRLKLLFVTDGT 420
           ELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEIS LWHDRL+LLFVTDGT
Sbjct: 361 ELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWHDRLRLLFVTDGT 420

Query: 421 LRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQ 478
           +RIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQD LERQ
Sbjct: 421 MRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDXLERQ 480

BLAST of Lag0027211 vs. ExPASy TrEMBL
Match: A0A5A7TRC7 (Fructokinase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001140 PE=4 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 3.6e-237
Identity = 428/513 (83.43%), Postives = 447/513 (87.13%), Query Frame = 0

Query: 1   MASFQL------LPHLQFSCFLPFYPSNCRAKAL----------------------IRAS 60
           MASF L      LPH+QFSCFLPFY S  RAK +                       RAS
Sbjct: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSTYRAKPIDLSLIFNLRHFQSSSKTLKLNPPRAS 60

Query: 61  -NGDGNGVVETPNPTRRGRKKGTKSSSSSTTTQKTAKRTKK-------TERNSFHQISED 120
            NG+GNGVVETP+PTRRGRKKGT S SSST+ QK  KR+KK       T  N F  ISED
Sbjct: 61  TNGEGNGVVETPSPTRRGRKKGTASPSSSTSAQKKTKRSKKPQITDKTTVANPFDHISED 120

Query: 121 EDEPIHLIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHPDKYSEWKMLQW 180
           E   I  IA+NYDDGMD PYEDPPLICCFGAAQKEFVPS+RVHDNQM  DKYSEWKMLQW
Sbjct: 121 ES--IDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMDADKYSEWKMLQW 180

Query: 181 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 240
           DPPEFARAPGGSPSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL
Sbjct: 181 DPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 240

Query: 241 NSRTACTYMKVKFEDGKLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAME 300
           NS+TACTYMK+KFEDGKLKMETVKEPAEDSL+SSELNLAVLKEARIFHFNSEAL+ST +E
Sbjct: 241 NSKTACTYMKIKFEDGKLKMETVKEPAEDSLLSSELNLAVLKEARIFHFNSEALLSTVIE 300

Query: 301 STLFKAIQLSKKFGGLIFFDLNLPLPLWRSRDETQEYIKKAWNEADIIEVSRQELEFLLD 360
            TLFKAIQLSKKFGGLIFFDLNLPLPLW+SR+ET+EYIKKAWNEADIIEVSRQELEFLLD
Sbjct: 301 PTLFKAIQLSKKFGGLIFFDLNLPLPLWKSREETREYIKKAWNEADIIEVSRQELEFLLD 360

Query: 361 EEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISSLWHDRLKLLFVTDGTLRIHYYS 420
           EEYYEKKRNYRPQYYAQTIEQTKNRRD+YHYTPEEIS LWHD LKLLFVTDGTLRIHYYS
Sbjct: 361 EEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDHLKLLFVTDGTLRIHYYS 420

Query: 421 PSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAVAA 478
           PSFHGV IGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM ENQDVLERQLRFA+AA
Sbjct: 421 PSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAA 480

BLAST of Lag0027211 vs. ExPASy TrEMBL
Match: A0A1S4DUM6 (fructokinase-like 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486334 PE=4 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 3.6e-237
Identity = 428/513 (83.43%), Postives = 447/513 (87.13%), Query Frame = 0

Query: 1   MASFQL------LPHLQFSCFLPFYPSNCRAKAL----------------------IRAS 60
           MASF L      LPH+QFSCFLPFY S  RAK +                       RAS
Sbjct: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSTYRAKPIDLSLIFNLRHFQSSSKTLKLNPPRAS 60

Query: 61  -NGDGNGVVETPNPTRRGRKKGTKSSSSSTTTQKTAKRTKK-------TERNSFHQISED 120
            NG+GNGVVETP+PTRRGRKKGT S SSST+ QK  KR+KK       T  N F  ISED
Sbjct: 61  TNGEGNGVVETPSPTRRGRKKGTASPSSSTSAQKKTKRSKKPQITDKTTVANPFDHISED 120

Query: 121 EDEPIHLIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHPDKYSEWKMLQW 180
           E   I  IA+NYDDGMD PYEDPPLICCFGAAQKEFVPS+RVHDNQM  DKYSEWKMLQW
Sbjct: 121 ES--IDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMDADKYSEWKMLQW 180

Query: 181 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 240
           DPPEFARAPGGSPSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL
Sbjct: 181 DPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 240

Query: 241 NSRTACTYMKVKFEDGKLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAME 300
           NS+TACTYMK+KFEDGKLKMETVKEPAEDSL+SSELNLAVLKEARIFHFNSEAL+ST +E
Sbjct: 241 NSKTACTYMKIKFEDGKLKMETVKEPAEDSLLSSELNLAVLKEARIFHFNSEALLSTVIE 300

Query: 301 STLFKAIQLSKKFGGLIFFDLNLPLPLWRSRDETQEYIKKAWNEADIIEVSRQELEFLLD 360
            TLFKAIQLSKKFGGLIFFDLNLPLPLW+SR+ET+EYIKKAWNEADIIEVSRQELEFLLD
Sbjct: 301 PTLFKAIQLSKKFGGLIFFDLNLPLPLWKSREETREYIKKAWNEADIIEVSRQELEFLLD 360

Query: 361 EEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISSLWHDRLKLLFVTDGTLRIHYYS 420
           EEYYEKKRNYRPQYYAQTIEQTKNRRD+YHYTPEEIS LWHD LKLLFVTDGTLRIHYYS
Sbjct: 361 EEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDHLKLLFVTDGTLRIHYYS 420

Query: 421 PSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAVAA 478
           PSFHGV IGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM ENQDVLERQLRFA+AA
Sbjct: 421 PSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAA 480

BLAST of Lag0027211 vs. ExPASy TrEMBL
Match: A0A0A0LA03 (PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G732620 PE=4 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 1.0e-236
Identity = 427/513 (83.24%), Postives = 447/513 (87.13%), Query Frame = 0

Query: 1   MASFQL------LPHLQFSCFLPFYPSNCRAKAL----------------------IRAS 60
           MASF L      LPH+QFSCFLPFY SN RAK +                       RAS
Sbjct: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSNYRAKPIDLSLIFNFHHSQSLSKTLKLNPPRAS 60

Query: 61  -NGDGNGVVETPNPTRRGRKKGTKSSSSSTTTQKTAKRTKK-------TERNSFHQISED 120
            N +GNGVVETP PTRRGRKKGT S SSST+ QK  KR+K+       T  N F  ISED
Sbjct: 61  ANDEGNGVVETPGPTRRGRKKGTASPSSSTSAQKKTKRSKEPQITDRITVANPFVHISED 120

Query: 121 EDEPIHLIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHPDKYSEWKMLQW 180
           E   I  IA+NYDDGMD PYEDPPLICCFGAAQKEFVPS+RVHDNQMH DKYSEWKMLQW
Sbjct: 121 ES--IDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQW 180

Query: 181 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 240
           DPPEFARAPGGSPSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL
Sbjct: 181 DPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 240

Query: 241 NSRTACTYMKVKFEDGKLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAME 300
           NS+TACTYMK+KFEDGKLK ETVKEPAEDSL+SSELNLAVLKEARIFHFNSE+L+STA+E
Sbjct: 241 NSKTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAIE 300

Query: 301 STLFKAIQLSKKFGGLIFFDLNLPLPLWRSRDETQEYIKKAWNEADIIEVSRQELEFLLD 360
            TLFKAIQLSKKFGGLIFFDLNLPLPLW+SRDET+EYIKKAW EADIIEVSRQELEFLLD
Sbjct: 301 PTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLLD 360

Query: 361 EEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISSLWHDRLKLLFVTDGTLRIHYYS 420
           EEYYEKKRNYRPQYYAQTIEQTKNRRD+YHYTPEE+S LWHDRLKLLFVTDGTLRIHYYS
Sbjct: 361 EEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYYS 420

Query: 421 PSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAVAA 478
           PSFHGV IGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM ENQDVLERQLRFA+AA
Sbjct: 421 PSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAA 480

BLAST of Lag0027211 vs. ExPASy TrEMBL
Match: A0A6J1I311 (fructokinase-like 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111470081 PE=4 SV=1)

HSP 1 Score: 826.2 bits (2133), Expect = 6.8e-236
Identity = 423/502 (84.26%), Postives = 443/502 (88.25%), Query Frame = 0

Query: 1   MASFQLL------PHLQFSCFLPFYPSNCRAKALI------------------RASNGDG 60
           MASFQLL      PHLQFS FL FYPS CR K LI                   ASN DG
Sbjct: 1   MASFQLLPATFSIPHLQFSRFLHFYPSICREKTLIFNSRYLQSSCKSLKLSPPSASNADG 60

Query: 61  NGVVETPNPTRRGRKKGTKSSSSST-TTQKTAKRTKKTERNSFHQISEDEDEPIHLIAEN 120
           N    TP PTRRGRKKGTKS SSST  ++KT    + T+ N F Q+S D+DE I  IAEN
Sbjct: 61  NEDTGTPTPTRRGRKKGTKSPSSSTKRSRKTLIEDELTQGNLFDQMSVDDDEAIDSIAEN 120

Query: 121 YDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHPDKYSEWKMLQWDPPEFARAPGG 180
           YDDGMDFPYEDPPLICCFGAAQKEFVPS+RVHDNQMHPDKYSEWKMLQWDPPEFARAPGG
Sbjct: 121 YDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPPEFARAPGG 180

Query: 181 SPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKV 240
           SPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR VKFDLNSRTACTYMK+
Sbjct: 181 SPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRGVKFDLNSRTACTYMKI 240

Query: 241 KFEDGKLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAMESTLFKAIQLSK 300
           KF+DGKLKMETVKE AEDSLVSSELNLAVLKEARIFHFNSEAL+ST ME+TLFKAIQLSK
Sbjct: 241 KFDDGKLKMETVKESAEDSLVSSELNLAVLKEARIFHFNSEALLSTTMEATLFKAIQLSK 300

Query: 301 KFGGLIFFDLNLPLPLWRSRDETQEYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYR 360
           KFGGLIFFDLNL LPLWRSRDET++ I+KAWN+ADIIEVSRQELEFLLDEEYYEKKRNYR
Sbjct: 301 KFGGLIFFDLNLSLPLWRSRDETRKCIEKAWNQADIIEVSRQELEFLLDEEYYEKKRNYR 360

Query: 361 PQYYAQTIEQTKNRRDYYHYTPEEISSLWHDRLKLLFVTDGTLRIHYYSPSFHGVVIGTE 420
           PQYYAQTIEQTKNRRD+YHY+PEEIS LWHD LKLL VTDGTLRIHYYSPSFHGVVIGTE
Sbjct: 361 PQYYAQTIEQTKNRRDHYHYSPEEISPLWHDHLKLLLVTDGTLRIHYYSPSFHGVVIGTE 420

Query: 421 DVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAVAAGIISQWTIGAV 478
           DVLITPFTCDRTGSGDA+VAGIMRKLTTFPEMFENQDVLERQLRFA+AAGIISQWTIGAV
Sbjct: 421 DVLITPFTCDRTGSGDALVAGIMRKLTTFPEMFENQDVLERQLRFAIAAGIISQWTIGAV 480

BLAST of Lag0027211 vs. TAIR 10
Match: AT3G54090.1 (fructokinase-like 1 )

HSP 1 Score: 682.9 bits (1761), Expect = 1.8e-196
Identity = 340/479 (70.98%), Postives = 398/479 (83.09%), Query Frame = 0

Query: 1   MASFQLLPHLQFSCFLPFYPSNCRAKALIRASNGDGNGVVETPNPTRRGRKKGTKSSSSS 60
           MAS  + PHL        + S+   + ++   +   +    TP  +  G   G  + +++
Sbjct: 1   MASLLIFPHLH------HFDSSLDRREVLVVRHSQASRRFLTPKASING--SGITNGAAA 60

Query: 61  TTTQKTAKRTKKTERNS--FHQISEDEDEPIHLIAENYDDGMDFPYEDPPLICCFGAAQK 120
            TT K +++ +K ++ S    + + + D  ++    +YDDG++FPY+DPPL+CCFGA QK
Sbjct: 61  ETTSKPSRKGRKKKQTSTVIEKDNTETDPELNPELADYDDGIEFPYDDPPLVCCFGAVQK 120

Query: 121 EFVPSVRVHDNQMHPDKYSEWKMLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVG 180
           EFVP VRVHDN MHPD YS+WKMLQWDPPEF RAPGG PSNVAISHVRLGGRAAFMGKVG
Sbjct: 121 EFVPVVRVHDNPMHPDMYSQWKMLQWDPPEFGRAPGGPPSNVAISHVRLGGRAAFMGKVG 180

Query: 181 KDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKVKFEDGKLKMETVKEPAEDSLVSS 240
           +DDFGDELVLMMN+E+VQTRAVKFD NS+TACT +K+KF+DGK+  ETVKEP EDSL +S
Sbjct: 181 EDDFGDELVLMMNQERVQTRAVKFDENSKTACTRVKIKFKDGKMMAETVKEPPEDSLFAS 240

Query: 241 ELNLAVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNLPLPLWRSRDET 300
           ELNLAVLKEARIFHFNSE L S  M+STLF AIQ SKKFGGLIFFDLNLPLPLWRSR+ET
Sbjct: 241 ELNLAVLKEARIFHFNSEVLTSPTMQSTLFTAIQWSKKFGGLIFFDLNLPLPLWRSRNET 300

Query: 301 QEYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPE 360
           ++ IKKAWNEA+IIEVS+QELEFLLDE+YYE++RNY PQY+A+  +QTKNRRDYYHYTPE
Sbjct: 301 RKLIKKAWNEANIIEVSQQELEFLLDEDYYERRRNYTPQYFAEDFDQTKNRRDYYHYTPE 360

Query: 361 EISSLWHDRLKLLFVTDGTLRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIM 420
           EI SLWHD+LKLL VTDGTLR+HYY+P+F GVVIGTEDVLITPFTCDRTGSGDAVVAGIM
Sbjct: 361 EIKSLWHDKLKLLVVTDGTLRLHYYTPTFDGVVIGTEDVLITPFTCDRTGSGDAVVAGIM 420

Query: 421 RKLTTFPEMFENQDVLERQLRFAVAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 478
           RKLTT PEMFE+QDV+ERQLRFAVAAGII+QWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 421 RKLTTCPEMFEDQDVMERQLRFAVAAGIIAQWTIGAVRGFPTESATQNLKEQVYVPSMW 471

BLAST of Lag0027211 vs. TAIR 10
Match: AT1G69200.1 (fructokinase-like 2 )

HSP 1 Score: 323.9 bits (829), Expect = 2.1e-88
Identity = 181/441 (41.04%), Postives = 263/441 (59.64%), Query Frame = 0

Query: 20  PSNCRAKALIRASNGDGNGVVETPNPTRRGRKKGTKSSSSSTTTQKTAKRTKKTERNSFH 79
           P   R +    A++ D   V       R+   K  K       T   A+ +   E  +  
Sbjct: 119 PKKTRTRKKAAAASSDVEEVKTEKKVRRKRTVKKDKDVEDDLATIMDAEVSDVEEALAVE 178

Query: 80  QI-SEDEDEPIHLIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHPDKYSE 139
              +E E+E I L     +D +   Y  PPL+CCFG+AQ  FVPS R  +  +  + +  
Sbjct: 179 STDTESEEEEIDLSKHEGED-ISHTYGWPPLVCCFGSAQHAFVPSGRPANRLLDYELHER 238

Query: 140 WKMLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR 199
            +  +W P ++ RAPGG    VAI+   LGG+ AFMGK+G DD+G  ++  +N  KVQTR
Sbjct: 239 MRDAKWAPEKYIRAPGGCAGGVAIALASLGGKVAFMGKLGADDYGQAMLYYLNVCKVQTR 298

Query: 200 AVKFDLNSRTACTYMKVKFEDGKLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEAL 259
           +VK D    TAC+ MK+  + G+LK   +K  AEDSL  SE+N+ VLKEA++F+F++ +L
Sbjct: 299 SVKIDGKRVTACSTMKIS-KRGRLKSTCIKPCAEDSLSKSEINVDVLKEAKMFYFSTHSL 358

Query: 260 MSTAMESTLFKAIQLSKKFGGLIFFDLNLPLPLWRSRDETQEYIKKAWNEADIIEVSRQE 319
           +   M ST  +AI++SK+ G +IF+DLNLPLPLW S +ET+ +I++ WN AD+IE+++QE
Sbjct: 359 LDKKMMSTTIQAIKISKQLGNVIFYDLNLPLPLWHSSEETKSFIQEVWNLADVIEITKQE 418

Query: 320 LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISSLWHDRLKLLFVTDGTL 379
           LEFL   E  E       ++  +  + +K    + HY PE +  LWH+ LK+LFVT+GT 
Sbjct: 419 LEFLCGIEPTE-------EFDTENNDISK----FVHYPPETVEQLWHENLKVLFVTNGTS 478

Query: 380 RIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQL 439
           +IHYY+   +G V G EDV ITPFT D + SGD +VAG++R LT  P++  N+  LER  
Sbjct: 479 KIHYYTKEHNGAVSGMEDVPITPFTRDMSASGDGIVAGLIRMLTVQPDLMNNKGYLERTA 538

Query: 440 RFAVAAGIISQWTIGAVRGFP 460
           R+A+  GII QW +   RG+P
Sbjct: 539 RYAIECGIIDQWLLAQTRGYP 546

BLAST of Lag0027211 vs. TAIR 10
Match: AT3G59480.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 194.9 bits (494), Expect = 1.5e-49
Identity = 125/363 (34.44%), Postives = 186/363 (51.24%), Query Frame = 0

Query: 109 LICCFGAAQKEFVPSVRVHDNQMHPDKYSEWKMLQWDPPEFARAPGGSPSNVAISHVRLG 168
           LI  FG    +FVP+V                +   D P F +APGG+P+NVAI+  RLG
Sbjct: 10  LIVSFGEMLIDFVPTVS--------------GVSLADAPGFIKAPGGAPANVAIAISRLG 69

Query: 169 GRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKVKFEDGKLKMETVK 228
           GRAAF+GK+G D+FG  L  ++ +  V    + FD  +RTA  ++ ++  DG+ +    +
Sbjct: 70  GRAAFVGKLGDDEFGHMLAGILKQNGVSAEGINFDTGARTALAFVTLR-SDGEREFMFYR 129

Query: 229 EPAEDSLV-SSELNLAVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNL 288
            P+ D L+   ELNL V++ A++FH+ S +L+     S   KA++++K+ G L+ +D NL
Sbjct: 130 NPSADMLLRPDELNLDVIRSAKVFHYGSISLIVEPCRSAHLKAMEVAKEAGALLSYDPNL 189

Query: 289 PLPLWRSRDETQEYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTK 348
            LPLW S++E Q+ I   W++A++I+VS +EL FL   +  +                  
Sbjct: 190 RLPLWPSKEEAQKQILSIWDKAEVIKVSDEELMFLTGSDKVD------------------ 249

Query: 349 NRRDYYHYTPEEISSLWHDRLKLLFVTDGTLRIHYYSPSFHGVVIGTEDVLITPF---TC 408
                     E   SLWH  LKLL VT G     YY+ SF G V         PF     
Sbjct: 250 ---------DETALSLWHSNLKLLLVTLGEKGCRYYTKSFRGSV--------DPFHVDAV 309

Query: 409 DRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAVAAGIISQWTIGAVRGFPTESAT 468
           D TG+GD+ V  ++ K+     + E++  L   LR A A G I+    GA+   PTES  
Sbjct: 310 DTTGAGDSFVGALLCKIVDDRAVLEDEARLREVLRLANACGAITTTKKGAIPALPTESEV 322

BLAST of Lag0027211 vs. TAIR 10
Match: AT1G06020.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 190.3 bits (482), Expect = 3.6e-48
Identity = 124/364 (34.07%), Postives = 190/364 (52.20%), Query Frame = 0

Query: 109 LICCFGAAQKEFVPSVRVHDNQMHPDKYSEWKMLQWDPPEFARAPGGSPSNVAISHVRLG 168
           LI  FG    +FVP+V                +   + P F +APGG+P+NVAI+  RLG
Sbjct: 10  LIVSFGEMLIDFVPTVS--------------GVSLSESPGFLKAPGGAPANVAIAVSRLG 69

Query: 169 GRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKVKFEDGKLKMETVK 228
           GRAAF+GK+G DDFG  L  ++ K  V  + + FD  +RTA  ++ ++  DG+ +    +
Sbjct: 70  GRAAFVGKLGDDDFGHMLAGILRKNGVDDQGINFDEGARTALAFVTLR-SDGEREFMFYR 129

Query: 229 EPAEDSLV-SSELNLAVLKEARIFHFNSEALMSTAMESTLFKAIQLSKKFGGLIFFDLNL 288
            P+ D L+   ELNL +++ A++FH+ S +L++    S   KA++++K+ G L+ +D NL
Sbjct: 130 NPSADMLLRPDELNLELIRSAKVFHYGSISLITEPCRSAHMKAMEVAKEAGALLSYDPNL 189

Query: 289 PLPLWRSRDETQEYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTK 348
             PLW S +E +  I   W++ADII+VS  ELEFL + +  + K                
Sbjct: 190 REPLWPSPEEARTQIMSIWDKADIIKVSDVELEFLTENKTMDDK---------------- 249

Query: 349 NRRDYYHYTPEEISSLWHDRLKLLFVTDGTLRIHYYSPSFHGVVIGTEDVLITPFTCDRT 408
                         SLWH  LKLL VT G     Y++  FHG V   E   +     D T
Sbjct: 250 -----------TAMSLWHPNLKLLLVTLGEKGCTYFTKKFHGSV---ETFHVD--AVDTT 309

Query: 409 GSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAVAAGIISQWTIGAVRGFPTE-SATQN 468
           G+GD+ V  +++++     + E++  L + LRFA A G I+    GA+   PT+  A   
Sbjct: 310 GAGDSFVGALLQQIVDDQSVLEDEARLRKVLRFANACGAITTTKKGAIPALPTDIEALSF 326

Query: 469 LKEQ 471
           LK+Q
Sbjct: 370 LKDQ 326

BLAST of Lag0027211 vs. TAIR 10
Match: AT1G06030.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 189.1 bits (479), Expect = 8.1e-48
Identity = 118/325 (36.31%), Postives = 178/325 (54.77%), Query Frame = 0

Query: 149 FARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRT 208
           F +APGG+P+NVAI+  RLGGRAAF+GK+G D+FG  L  ++ K  V  + + FD  +RT
Sbjct: 37  FLKAPGGAPANVAIAVSRLGGRAAFVGKLGDDEFGHMLAGILRKNDVDDQGINFDKGART 96

Query: 209 ACTYMKVKFEDGKLKMETVKEPAEDSLV-SSELNLAVLKEARIFHFNSEALMSTAMESTL 268
           A  ++ ++  DG+ +    + P+ D L+   ELNL +++ A++FH+ S +L++    S  
Sbjct: 97  ALAFVTLR-SDGEREFMFYRNPSADMLLRPDELNLELIRSAKVFHYGSISLITEPCRSAH 156

Query: 269 FKAIQLSKKFGGLIFFDLNLPLPLWRSRDETQEYIKKAWNEADIIEVSRQELEFLLDEEY 328
            KA++++K+ G L+ +D NL  PLW S +E ++ I   W++ADII+VS  ELEFL     
Sbjct: 157 MKAMEVAKEAGALLSYDPNLREPLWPSPEEARKQIMSIWDKADIIKVSDVELEFLTGN-- 216

Query: 329 YEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISSLWHDRLKLLFVTDGTLRIHYYSPSF 388
                        +TI+             E   SLWH  LKLL VT G     YY+  F
Sbjct: 217 -------------KTIDD------------ETAMSLWHPNLKLLLVTLGENGCRYYTKDF 276

Query: 389 HGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAVAAGII 448
           HG V   E   +     D TG+GD+ V  ++ ++     + E ++ L + LRFA A G I
Sbjct: 277 HGSV---ETFHVD--AVDTTGAGDSFVGALLNQIVDDQSVLEEEERLRKVLRFANACGAI 328

Query: 449 SQWTIGAVRGFPTE-SATQNLKEQV 472
           +    GA+   PT+  A   LK QV
Sbjct: 337 TTTKKGAIPALPTDCEALSFLKIQV 328