Lag0027205 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0027205
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Copper transporter
Location	chr10: 45844453 .. 45844911 (-)
RNA-Seq Expression	Lag0027205
Synteny	Lag0027205

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGAAGGATCTGAAAACGACGTTGCATCATAACCTCGGCGGCGTGGCGCCACCGGCGGCGGAGACCAATGCCGGGAAGACGGTGGTGCACAAGTCATTGTACTGGGGGCACGAAGCGCAAGTCCTGTTCAGCGGGTGGCCCGGTGCGAACTCCGGCATGTACGCATTGGCGGTGATGTTGGTGTTCGTTTTAGCGGTGCTAGTGGAATGGCTTACTTATTTAAAGTTGATGAAGGAAAGCGGGGAGGATGTGTGGAGGGTGGTGGTGCAGACGGCGATACACGCAGTACGTACTGGATTGAGTTACATGGTGATGCTATCGGTGATGTCGTTCAACGGCGGGATCTTCCTGGCTGCGGTGGGTGGGCACGCTGTGGGGTTCGTTCTGTTCAGAGCCCGAGCCAAACAAAAGAGCGAGTCAGATTCGCGGAAGCCCAATGGTCCAATGACATTCTGA

mRNA sequence

ATGATGAAGGATCTGAAAACGACGTTGCATCATAACCTCGGCGGCGTGGCGCCACCGGCGGCGGAGACCAATGCCGGGAAGACGGTGGTGCACAAGTCATTGTACTGGGGGCACGAAGCGCAAGTCCTGTTCAGCGGGTGGCCCGGTGCGAACTCCGGCATGTACGCATTGGCGGTGATGTTGGTGTTCGTTTTAGCGGTGCTAGTGGAATGGCTTACTTATTTAAAGTTGATGAAGGAAAGCGGGGAGGATGTGTGGAGGGTGGTGGTGCAGACGGCGATACACGCAGTACGTACTGGATTGAGTTACATGGTGATGCTATCGGTGATGTCGTTCAACGGCGGGATCTTCCTGGCTGCGGTGGGTGGGCACGCTGTGGGGTTCGTTCTGTTCAGAGCCCGAGCCAAACAAAAGAGCGAGTCAGATTCGCGGAAGCCCAATGGTCCAATGACATTCTGA

Coding sequence (CDS)

ATGATGAAGGATCTGAAAACGACGTTGCATCATAACCTCGGCGGCGTGGCGCCACCGGCGGCGGAGACCAATGCCGGGAAGACGGTGGTGCACAAGTCATTGTACTGGGGGCACGAAGCGCAAGTCCTGTTCAGCGGGTGGCCCGGTGCGAACTCCGGCATGTACGCATTGGCGGTGATGTTGGTGTTCGTTTTAGCGGTGCTAGTGGAATGGCTTACTTATTTAAAGTTGATGAAGGAAAGCGGGGAGGATGTGTGGAGGGTGGTGGTGCAGACGGCGATACACGCAGTACGTACTGGATTGAGTTACATGGTGATGCTATCGGTGATGTCGTTCAACGGCGGGATCTTCCTGGCTGCGGTGGGTGGGCACGCTGTGGGGTTCGTTCTGTTCAGAGCCCGAGCCAAACAAAAGAGCGAGTCAGATTCGCGGAAGCCCAATGGTCCAATGACATTCTGA

Protein sequence

MMKDLKTTLHHNLGGVAPPAAETNAGKTVVHKSLYWGHEAQVLFSGWPGANSGMYALAVMLVFVLAVLVEWLTYLKLMKESGEDVWRVVVQTAIHAVRTGLSYMVMLSVMSFNGGIFLAAVGGHAVGFVLFRARAKQKSESDSRKPNGPMTF

Homology

[Close]

BLAST of Lag0027205 vs. NCBI nr
Match: KAG6596378.1 (Copper transporter 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 240.7 bits (613), Expect = 8.0e-60
Identity = 121/157 (77.07%), Postives = 134/157 (85.35%), Query Frame = 0


Query: 2   MKDLKTTLHHNLGGVAPPAAET------NAGKTVVHKSLYWGHEAQVLFSGWPGANSGMY 61
           M  LKTT HHNLG V PPA E+      NAGKTVVHK+LYWGH+AQVLF+GWPG NS MY
Sbjct: 1   MMALKTTSHHNLGAVPPPAVESNPATTNNAGKTVVHKTLYWGHDAQVLFTGWPGTNSAMY 60

Query: 62  ALAVMLVFVLAVLVEWLTYLKLMKESGEDVWRVVVQTAIHAVRTGLSYMVMLSVMSFNGG 121
           ALAV+ VFVLAV VEWLTYL  MKES ++V  VV+QTAIH VRTGL+YMV+LSVMSFNGG
Sbjct: 61  ALAVIFVFVLAVFVEWLTYLNFMKESADNVLTVVLQTAIHTVRTGLNYMVILSVMSFNGG 120

Query: 122 IFLAAVGGHAVGFVLFRARAKQKSESDSRKPNGPMTF 153
           IFLAAVGGHAVGFVLFRARAK+K+ESDS KPNGP+TF
Sbjct: 121 IFLAAVGGHAVGFVLFRARAKRKAESDSPKPNGPLTF 157

[Close]

BLAST of Lag0027205 vs. NCBI nr
Match: KAG7027927.1 (Copper transporter 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 240.0 bits (611), Expect = 1.4e-59
Identity = 120/154 (77.92%), Postives = 133/154 (86.36%), Query Frame = 0


Query: 5   LKTTLHHNLGGVAPPAAET------NAGKTVVHKSLYWGHEAQVLFSGWPGANSGMYALA 64
           LKTT HHNLG V PPA E+      NAGKTVVHK+LYWGH+AQVLF+GWPG NS MYALA
Sbjct: 3   LKTTSHHNLGAVPPPAVESNPATTNNAGKTVVHKTLYWGHDAQVLFTGWPGTNSAMYALA 62

Query: 65  VMLVFVLAVLVEWLTYLKLMKESGEDVWRVVVQTAIHAVRTGLSYMVMLSVMSFNGGIFL 124
           V+ VFVLAV VEWLTYL  MKES ++V  VV+QTAIH VRTGL+YMV+LSVMSFNGGIFL
Sbjct: 63  VIFVFVLAVFVEWLTYLNFMKESADNVLTVVLQTAIHTVRTGLNYMVILSVMSFNGGIFL 122

Query: 125 AAVGGHAVGFVLFRARAKQKSESDSRKPNGPMTF 153
           AAVGGHAVGFVLFRARAK+K+ESDS KPNGP+TF
Sbjct: 123 AAVGGHAVGFVLFRARAKRKAESDSPKPNGPLTF 156

[Close]

BLAST of Lag0027205 vs. NCBI nr
Match: XP_022941866.1 (copper transporter 4-like [Cucurbita moschata])

HSP 1 Score: 240.0 bits (611), Expect = 1.4e-59
Identity = 121/157 (77.07%), Postives = 134/157 (85.35%), Query Frame = 0


Query: 2   MKDLKTTLHHNLGGVAPPAAET------NAGKTVVHKSLYWGHEAQVLFSGWPGANSGMY 61
           M  LKTT HHNLG V PPA ET      NAGKTVVHK+LYWGH+AQVLF+GWP  NS MY
Sbjct: 1   MMALKTTSHHNLGAVPPPAVETNPATTNNAGKTVVHKTLYWGHDAQVLFTGWPSTNSAMY 60

Query: 62  ALAVMLVFVLAVLVEWLTYLKLMKESGEDVWRVVVQTAIHAVRTGLSYMVMLSVMSFNGG 121
           ALAV+ VFVLAV VEWLTYL  MKES ++V  VV+QTAIHAVRTGL+YMV+LSVMSFNGG
Sbjct: 61  ALAVIFVFVLAVFVEWLTYLNFMKESADNVLTVVLQTAIHAVRTGLNYMVILSVMSFNGG 120

Query: 122 IFLAAVGGHAVGFVLFRARAKQKSESDSRKPNGPMTF 153
           IFLAAVGGHAVGFVLFRARAK+K++SDS KPNGP+TF
Sbjct: 121 IFLAAVGGHAVGFVLFRARAKRKAKSDSPKPNGPLTF 157

[Close]

BLAST of Lag0027205 vs. NCBI nr
Match: XP_022971334.1 (copper transporter 6-like [Cucurbita maxima])

HSP 1 Score: 240.0 bits (611), Expect = 1.4e-59
Identity = 120/157 (76.43%), Postives = 134/157 (85.35%), Query Frame = 0


Query: 2   MKDLKTTLHHNLGGVAPPAAET------NAGKTVVHKSLYWGHEAQVLFSGWPGANSGMY 61
           M  LKTT HHNLGGV PPA E+      NAGKTVVHK+LYWGH+AQVLF+GWPG NS MY
Sbjct: 80  MMALKTTSHHNLGGVPPPAVESNPANTNNAGKTVVHKTLYWGHDAQVLFTGWPGTNSAMY 139

Query: 62  ALAVMLVFVLAVLVEWLTYLKLMKESGEDVWRVVVQTAIHAVRTGLSYMVMLSVMSFNGG 121
           ALAV+ VFVLAV VEWLTYL  MK+S ++V  VV+Q+AIHAVRTGL YMVMLSVMSFNGG
Sbjct: 140 ALAVIFVFVLAVFVEWLTYLNFMKQSADNVLTVVLQSAIHAVRTGLKYMVMLSVMSFNGG 199

Query: 122 IFLAAVGGHAVGFVLFRARAKQKSESDSRKPNGPMTF 153
           IFLAAVGGHAVGFVLFR RA++K+ESDS KPNGP+TF
Sbjct: 200 IFLAAVGGHAVGFVLFRGRAERKAESDSPKPNGPLTF 236

[Close]

BLAST of Lag0027205 vs. NCBI nr
Match: XP_022145715.1 (copper transporter 4 [Momordica charantia])

HSP 1 Score: 236.1 bits (601), Expect = 2.0e-58
Identity = 121/152 (79.61%), Postives = 131/152 (86.18%), Query Frame = 0


Query: 1   MMKDLKTTLHHNLGGVAPPAAET-NAGKTVVHKSLYWGHEAQVLFSGWPGANSGMYALAV 60
           M   LK T  HNLG V PPA ET NAGKTVVHKSLYW H+AQVLF+GWPGA+S MYALAV
Sbjct: 9   MTLKLKATSRHNLGTVPPPATETNNAGKTVVHKSLYWSHDAQVLFTGWPGADSAMYALAV 68

Query: 61  MLVFVLAVLVEWLTYLKLMKESGEDVWRVVVQTAIHAVRTGLSYMVMLSVMSFNGGIFLA 120
           + VFVLAVLVEWLTY   MK+S E V + V+QTAIHAVRTGLSYMVMLSVMSFNGGIFLA
Sbjct: 69  IFVFVLAVLVEWLTYCNFMKQSAESVSKAVLQTAIHAVRTGLSYMVMLSVMSFNGGIFLA 128

Query: 121 AVGGHAVGFVLFRARAKQKSESDSRKPNGPMT 152
           AVGGHAVGFVLFRARAK+KSESDS KPNGP++
Sbjct: 129 AVGGHAVGFVLFRARAKRKSESDSTKPNGPIS 160

[Close]

BLAST of Lag0027205 vs. ExPASy Swiss-Prot
Match: Q8GWP3 (Copper transporter 6 OS=Arabidopsis thaliana OX=3702 GN=COPT6 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 8.4e-28
Identity = 62/140 (44.29%), Postives = 97/140 (69.29%), Query Frame = 0


Query: 10  HHNLGGVAPPAA--ETNAGKTVVHKSLYWGHEAQVLFSGWPGANSGMYALAVMLVFVLAV 69
           H N+   +P +    TN+   ++H + +WG   ++LFSGWPG + GMY L +++VF+LAV
Sbjct: 3   HGNMPPSSPSSMVNHTNSNMIMMHMTFFWGKNTEILFSGWPGTSLGMYVLCLIVVFLLAV 62

Query: 70  LVEWLTYLKLMKESGE-DVWRVVVQTAIHAVRTGLSYMVMLSVMSFNGGIFLAAVGGHAV 129
           +VEWL +  +++  G     + +VQTA++ ++TGL+Y+VML+VMSFNGG+F+ A+ G AV
Sbjct: 63  IVEWLAHSSILRGRGSTSRAKGLVQTAVYTLKTGLAYLVMLAVMSFNGGVFIVAIAGFAV 122

Query: 130 GFVLFRARAKQKSESDSRKP 147
           GF+LF + A  K+ SD  KP
Sbjct: 123 GFMLFGSTA-FKNPSDDEKP 141

[Close]

BLAST of Lag0027205 vs. ExPASy Swiss-Prot
Match: Q39065 (Copper transporter 1 OS=Arabidopsis thaliana OX=3702 GN=COPT1 PE=2 SV=2)

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-27
Identity = 63/124 (50.81%), Postives = 90/124 (72.58%), Query Frame = 0


Query: 27  KTVVHKSLYWGHEAQVLFSGWPGANSGMYALAVMLVFVLAVLVEWLTYLKLMKES-GEDV 86
           K ++H + +WG   +VLFSGWPG +SGMYAL ++ VF LAVL EWL +  L++ S G+  
Sbjct: 39  KMMMHMTFFWGKNTEVLFSGWPGTSSGMYALCLIFVFFLAVLTEWLAHSSLLRGSTGDSA 98

Query: 87  WRV--VVQTAIHAVRTGLSYMVMLSVMSFNGGIFLAAVGGHAVGFVLFRARAKQKSESDS 146
            R   ++QTA++ +R GL+Y+VML+VMSFN G+FL A+ GHAVGF+LF ++   ++ SD 
Sbjct: 99  NRAAGLIQTAVYTLRIGLAYLVMLAVMSFNAGVFLVALAGHAVGFMLFGSQT-FRNTSDD 158

Query: 147 RKPN 148
           RK N
Sbjct: 159 RKTN 161

[Close]

BLAST of Lag0027205 vs. ExPASy Swiss-Prot
Match: Q8SAA5 (Copper transporter 4 OS=Arabidopsis thaliana OX=3702 GN=COPT4 PE=2 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 2.7e-26
Identity = 56/111 (50.45%), Postives = 84/111 (75.68%), Query Frame = 0


Query: 28  TVVHKSLYWGHEAQVLFSGWPGANSGMYALAVMLVFVLAVLVEWL---TYLKLMKESGED 87
           +++H + YWG+  QVLFSGWPG++ GMYALA++ VF LA L EWL   +    +K+  + 
Sbjct: 28  SLLHPTFYWGYNCQVLFSGWPGSDRGMYALALIFVFFLAFLAEWLARCSDASSIKQGADK 87

Query: 88  VWRVVVQTAIHAVRTGLSYMVMLSVMSFNGGIFLAAVGGHAVGFVLFRARA 136
           + +V  +TA++ V++G SY+V+L+V+SFNGG+FLAA+ GHA+GF +FR RA
Sbjct: 88  LAKVAFRTAMYTVKSGFSYLVILAVVSFNGGVFLAAIFGHALGFAVFRGRA 138

[Close]

BLAST of Lag0027205 vs. ExPASy Swiss-Prot
Match: Q9STG2 (Copper transporter 2 OS=Arabidopsis thaliana OX=3702 GN=COPT2 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 4.6e-26
Identity = 57/143 (39.86%), Postives = 91/143 (63.64%), Query Frame = 0


Query: 10  HHNLGGVAPPAA-------ETNAGKTVVHKSLYWGHEAQVLFSGWPGANSGMYALAVMLV 69
           H ++  + PP+         T     ++H + +WG   +VLFSGWPG +SGMYAL ++++
Sbjct: 3   HDHMHDMPPPSPSSSSMSNHTTPHMMMMHMTFFWGKNTEVLFSGWPGTSSGMYALCLIVI 62

Query: 70  FVLAVLVEWLTYLKLMKESGEDVWRV-VVQTAIHAVRTGLSYMVMLSVMSFNGGIFLAAV 129
           F+LAV+ EWL +  +++ SG       + QTA++ ++TGLSY+VML+VMSFN G+F+ A+
Sbjct: 63  FLLAVIAEWLAHSPILRVSGSTNRAAGLAQTAVYTLKTGLSYLVMLAVMSFNAGVFIVAI 122

Query: 130 GGHAVGFVLFRARAKQKSESDSR 145
            G+ VGF LF +   +K   D +
Sbjct: 123 AGYGVGFFLFGSTTFKKPSDDQK 145

[Close]

BLAST of Lag0027205 vs. ExPASy Swiss-Prot
Match: Q5ZD08 (Copper transporter 3 OS=Oryza sativa subsp. japonica OX=39947 GN=COPT3 PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 8.7e-25
Identity = 61/126 (48.41%), Postives = 83/126 (65.87%), Query Frame = 0


Query: 11  HNLGGVAPPAAETNAG--KTVVHKSLYWGHEAQVLFSGWPGANSGMYALAVMLVFVLAVL 70
           H +     PAA   AG  K  +H + YWG  +++LF+GWPGA+ GMYALA+  VF LAVL
Sbjct: 7   HGMAMAMAPAAAGGAGRRKRYMHMTFYWGKNSEILFTGWPGASGGMYALALAAVFALAVL 66

Query: 71  VEWLTYLKLMKESG-EDVWRVVVQTAIHAVRTGLSYMVMLSVMSFNGGIFLAAVGGHAVG 130
           +E+L   ++ + S      R     A+HAVR GL+Y++ML++MSFN G+ LAAV GHA G
Sbjct: 67  LEFLGSPRVQESSSLGSRRRRATAAAVHAVRVGLAYLLMLALMSFNVGVLLAAVAGHAAG 126

Query: 131 FVLFRA 134
           F+ FRA
Sbjct: 127 FLAFRA 132

The following BLAST results are available for this feature:

BLAST of Lag0027205 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6596378.1	8.0e-60	77.07	Copper transporter 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7027927.1	1.4e-59	77.92	Copper transporter 4, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022941866.1	1.4e-59	77.07	copper transporter 4-like [Cucurbita moschata]	[more]
XP_022971334.1	1.4e-59	76.43	copper transporter 6-like [Cucurbita maxima]	[more]
XP_022145715.1	2.0e-58	79.61	copper transporter 4 [Momordica charantia]	[more]

BLAST of Lag0027205 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GWP3	8.4e-28	44.29	Copper transporter 6 OS=Arabidopsis thaliana OX=3702 GN=COPT6 PE=2 SV=1	[more]
Q39065	1.1e-27	50.81	Copper transporter 1 OS=Arabidopsis thaliana OX=3702 GN=COPT1 PE=2 SV=2	[more]
Q8SAA5	2.7e-26	50.45	Copper transporter 4 OS=Arabidopsis thaliana OX=3702 GN=COPT4 PE=2 SV=2	[more]
Q9STG2	4.6e-26	39.86	Copper transporter 2 OS=Arabidopsis thaliana OX=3702 GN=COPT2 PE=2 SV=1	[more]
Q5ZD08	8.7e-25	48.41	Copper transporter 3 OS=Oryza sativa subsp. japonica OX=39947 GN=COPT3 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007274	Ctr copper transporter	PFAM	PF04145	Ctr	coord: 86..131 e-value: 4.7E-12 score: 46.7 coord: 35..77 e-value: 1.3E-6 score: 29.0
IPR007274	Ctr copper transporter	PANTHER	PTHR12483	SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS	coord: 22..142
None	No IPR available	PANTHER	PTHR12483:SF94	COPPER TRANSPORTER 4	coord: 22..142

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0027205.1	Lag0027205.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006878	cellular copper ion homeostasis
biological_process	GO:0035434	copper ion transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005375	copper ion transmembrane transporter activity