Lag0027203 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0027203
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Adenine nucleotide alpha hydrolases-like superfamily protein
Location	chr10: 45820909 .. 45821648 (-)
RNA-Seq Expression	Lag0027203
Synteny	Lag0027203

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAAGGATCGGAAGATCGGAGTGGCTCTTGATTTCTCCAACAGCAGCAAAAACGCTCTCAAATGGGCCATCGATAACTTGGCCGACAAAGGCGACACTCTCTACATCATCTACGTTAACCCCAATTCACTCGACGAATCTGCCCATCACCTCTGGGCTCAATCCGGCTCTCGTCAGTAATCAATCCTCCTTTTTCTTCTCTTTATCTTTTCTTCTCTAAATCTTGCTCATCCGATTGCCTTTTTGGCAGCTCTGATTCCCTTGTCGGAATTCCGAGAGCCCGATGTTTTGAAGAACTACGATGTCAAAATCGACATCGAAGCCCTCGATATTCTCGACACTGGCTCTAGACAGAAAGAGGTACTGGTTGGTTTTCATCTTCTGCCCAAGTATAATAATGGTTCAATCAGTGTTTCGTTTTGAAATTAACCTGTGATTTTAGATTACCGTTGTTTCGAAGCTGTACTGGGGTGATGCGAGGGAGAAGATCATCGATGCGATTGAAGATCTGAAGCTGGATTCTCTGGTAATGGGAAGCAGAGGACTCAGTACCATTCGAAGGTTTGATTTCTTTCTTTCTCCTCTTCTCAGAACGAATCGGATTTAATTTCATGTTAGTTTTTCTGTTTGGTTGATCGGAAAACAGGATTCTGCTGGGGAGTGTTAGCAATTACGTGATTACTCATTCTCCTTGCCCTGTGACCGTCGTGAAGGATTCAAACTTCCAGCATCATTAA

mRNA sequence

ATGGTGAAGGATCGGAAGATCGGAGTGGCTCTTGATTTCTCCAACAGCAGCAAAAACGCTCTCAAATGGGCCATCGATAACTTGGCCGACAAAGGCGACACTCTCTACATCATCTACGTTAACCCCAATTCACTCGACGAATCTGCCCATCACCTCTGGGCTCAATCCGGCTCTCCTCTGATTCCCTTGTCGGAATTCCGAGAGCCCGATGTTTTGAAGAACTACGATGTCAAAATCGACATCGAAGCCCTCGATATTCTCGACACTGGCTCTAGACAGAAAGAGATTACCGTTGTTTCGAAGCTGTACTGGGGTGATGCGAGGGAGAAGATCATCGATGCGATTGAAGATCTGAAGCTGGATTCTCTGGTAATGGGAAGCAGAGGACTCAGTACCATTCGAAGGATTCTGCTGGGGAGTGTTAGCAATTACGTGATTACTCATTCTCCTTGCCCTGTGACCGTCGTGAAGGATTCAAACTTCCAGCATCATTAA

Coding sequence (CDS)

ATGGTGAAGGATCGGAAGATCGGAGTGGCTCTTGATTTCTCCAACAGCAGCAAAAACGCTCTCAAATGGGCCATCGATAACTTGGCCGACAAAGGCGACACTCTCTACATCATCTACGTTAACCCCAATTCACTCGACGAATCTGCCCATCACCTCTGGGCTCAATCCGGCTCTCCTCTGATTCCCTTGTCGGAATTCCGAGAGCCCGATGTTTTGAAGAACTACGATGTCAAAATCGACATCGAAGCCCTCGATATTCTCGACACTGGCTCTAGACAGAAAGAGATTACCGTTGTTTCGAAGCTGTACTGGGGTGATGCGAGGGAGAAGATCATCGATGCGATTGAAGATCTGAAGCTGGATTCTCTGGTAATGGGAAGCAGAGGACTCAGTACCATTCGAAGGATTCTGCTGGGGAGTGTTAGCAATTACGTGATTACTCATTCTCCTTGCCCTGTGACCGTCGTGAAGGATTCAAACTTCCAGCATCATTAA

Protein sequence

MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGSPLIPLSEFREPDVLKNYDVKIDIEALDILDTGSRQKEITVVSKLYWGDAREKIIDAIEDLKLDSLVMGSRGLSTIRRILLGSVSNYVITHSPCPVTVVKDSNFQHH

Homology

[Close]

BLAST of Lag0027203 vs. NCBI nr
Match: XP_038903504.1 (universal stress protein PHOS32 [Benincasa hispida])

HSP 1 Score: 315.5 bits (807), Expect = 2.8e-82
Identity = 154/164 (93.90%), Postives = 161/164 (98.17%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGSPL 60
           MVKDRKIGVALDFSNSSKNAL+WAIDNLADKGDTL+IIYVNPNSLDESAH LWA+SGSPL
Sbjct: 1   MVKDRKIGVALDFSNSSKNALRWAIDNLADKGDTLFIIYVNPNSLDESAHRLWAESGSPL 60

Query: 61  IPLSEFREPDVLKNYDVKIDIEALDILDTGSRQKEITVVSKLYWGDAREKIIDAIEDLKL 120
           IPLSEFREPDVLK YDVKIDIEALD LDTG+RQKEITVVSKLYWGDAREKI+DAIEDLKL
Sbjct: 61  IPLSEFREPDVLKKYDVKIDIEALDTLDTGARQKEITVVSKLYWGDAREKIVDAIEDLKL 120

Query: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHSPCPVTVVKDSNFQHH 165
           DSLVMGSRGLSTIRRILLGSVSNYVITH+PCPVTVVK+SNFQHH
Sbjct: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKESNFQHH 164

[Close]

BLAST of Lag0027203 vs. NCBI nr
Match: XP_011651876.1 (universal stress protein PHOS32 [Cucumis sativus] >KGN58822.1 hypothetical protein Csa_001140 [Cucumis sativus])

HSP 1 Score: 313.5 bits (802), Expect = 1.1e-81
Identity = 153/164 (93.29%), Postives = 161/164 (98.17%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGSPL 60
           MVKDRKIGVALDFSNSSKNAL+WAIDNLADKGDTL+IIYVNPNSL+ESAH LWA+SGSPL
Sbjct: 1   MVKDRKIGVALDFSNSSKNALRWAIDNLADKGDTLFIIYVNPNSLEESAHRLWAESGSPL 60

Query: 61  IPLSEFREPDVLKNYDVKIDIEALDILDTGSRQKEITVVSKLYWGDAREKIIDAIEDLKL 120
           IPLSEFREP+VLK YDVKIDIEALDILDTG+RQKEITVVSKLYWGDAREKI+DAIEDLKL
Sbjct: 61  IPLSEFREPEVLKKYDVKIDIEALDILDTGARQKEITVVSKLYWGDAREKIVDAIEDLKL 120

Query: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHSPCPVTVVKDSNFQHH 165
           DSLVMGSRGLSTIRRILLGSVSNYVITH+PCPVTVVKDSNF HH
Sbjct: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKDSNFPHH 164

[Close]

BLAST of Lag0027203 vs. NCBI nr
Match: XP_008442491.1 (PREDICTED: uncharacterized protein C167.05 [Cucumis melo] >KAA0044129.1 universal stress protein PHOS32-like [Cucumis melo var. makuwa] >TYK25009.1 universal stress protein PHOS32-like [Cucumis melo var. makuwa])

HSP 1 Score: 312.8 bits (800), Expect = 1.8e-81
Identity = 153/164 (93.29%), Postives = 161/164 (98.17%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGSPL 60
           MVKDRKIGVALDFS+SSKNALKWAIDNLADKGDTLYIIYVNPNSL+ESAH LWA+SGSPL
Sbjct: 1   MVKDRKIGVALDFSDSSKNALKWAIDNLADKGDTLYIIYVNPNSLNESAHRLWAESGSPL 60

Query: 61  IPLSEFREPDVLKNYDVKIDIEALDILDTGSRQKEITVVSKLYWGDAREKIIDAIEDLKL 120
           IPLSEFREP+VLK YDVKIDI+ALDILDTG+RQKEITVVSKLYWGDAREKI+DAIEDLKL
Sbjct: 61  IPLSEFREPEVLKKYDVKIDIDALDILDTGARQKEITVVSKLYWGDAREKIVDAIEDLKL 120

Query: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHSPCPVTVVKDSNFQHH 165
           DSLVMGSRGLSTIRRILLGSVSNYVITH+PCPVTVVKDSNF HH
Sbjct: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKDSNFPHH 164

[Close]

BLAST of Lag0027203 vs. NCBI nr
Match: XP_022145652.1 (universal stress protein PHOS32 [Momordica charantia])

HSP 1 Score: 305.4 bits (781), Expect = 2.9e-79
Identity = 148/164 (90.24%), Postives = 159/164 (96.95%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGSPL 60
           MVKDRK GVALDFSNSSKNAL+WAIDNLADKGDTL++IYVNPNSLDESAH LWA+SGSPL
Sbjct: 1   MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL 60

Query: 61  IPLSEFREPDVLKNYDVKIDIEALDILDTGSRQKEITVVSKLYWGDAREKIIDAIEDLKL 120
           IPLSEFREPDVL+ YDVKIDIEALDILDTG+RQKEI VV+KLYWGDAREKIIDAIEDLKL
Sbjct: 61  IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL 120

Query: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHSPCPVTVVKDSNFQHH 165
           +SLVMGSRGLSTIRRILLGSVSNYVITH+PCPVTVVK++NFQ H
Sbjct: 121 ESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKEANFQSH 164

[Close]

BLAST of Lag0027203 vs. NCBI nr
Match: XP_022971337.1 (universal stress protein PHOS32-like [Cucurbita maxima])

HSP 1 Score: 298.9 bits (764), Expect = 2.7e-77
Identity = 148/164 (90.24%), Postives = 157/164 (95.73%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGSPL 60
           MVKDRKIGVALDFSNSSKNALKWAIDNLADKGD LYIIYVNP SLD+SA  L A+SGSPL
Sbjct: 27  MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDILYIIYVNPKSLDQSAPQLSAESGSPL 86

Query: 61  IPLSEFREPDVLKNYDVKIDIEALDILDTGSRQKEITVVSKLYWGDAREKIIDAIEDLKL 120
           IPLS+FREP+VLK YDV+IDIEALDILDTG RQKEITVVSKLYWGDAREK++DAIEDLKL
Sbjct: 87  IPLSQFREPEVLKKYDVEIDIEALDILDTGCRQKEITVVSKLYWGDAREKLLDAIEDLKL 146

Query: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHSPCPVTVVKDSNFQHH 165
           DSLVMGSRGLSTIRRILLGSVSNYVITH+PCPVTVVKDSNF+HH
Sbjct: 147 DSLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKDSNFKHH 190

[Close]

BLAST of Lag0027203 vs. ExPASy Swiss-Prot
Match: Q8VYN9 (Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.2e-13
Identity = 52/163 (31.90%), Postives = 85/163 (52.15%), Query Frame = 0


Query: 5   RKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGSPLIPLS 64
           RKIGVA+D S  S  A++WA+D+    GD + +++V+P S+      L+     PL   +
Sbjct: 48  RKIGVAVDLSEESSFAVRWAVDHYIRPGDAVVLLHVSPTSV------LFGADWGPLPLKT 107

Query: 65  EFREPDVLKNYDVKIDIEAL---DILDTGSRQKEITVVSKLYW---GDAREKIIDAIEDL 124
           +  +P+  +    + D +A     + D     KE+    K++     D RE++   IE L
Sbjct: 108 QIEDPNA-QPQPSQEDFDAFTSTKVADLAKPLKELGFPYKIHIVKDHDMRERLCLEIERL 167

Query: 125 KLDSLVMGSRGLSTIRRI----LLGSVSNYVITHSPCPVTVVK 158
            L +++MGSRG    ++      LGSVS+Y + H  CPV VV+
Sbjct: 168 GLSAVIMGSRGFGAEKKRGSDGKLGSVSDYCVHHCVCPVVVVR 203

[Close]

BLAST of Lag0027203 vs. ExPASy Swiss-Prot
Match: Q8L4N1 (Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 7.0e-12
Identity = 55/167 (32.93%), Postives = 81/167 (48.50%), Query Frame = 0


Query: 5   RKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGSPLIPLS 64
           RKIGVA+D S  S  A++WA+D+    GD + I++V+P S+      L+     PL PL 
Sbjct: 45  RKIGVAVDLSEESAFAVRWAVDHYIRPGDAVVILHVSPTSV------LFGADWGPL-PLQ 104

Query: 65  EFREPDVLKNYDV-----KIDIEAL---DILDTGSRQKEITVVSKLYW---GDAREKIID 124
               P    +        + D +A     + D     KE     K++     D RE++  
Sbjct: 105 TPPPPSAATDPGAQPKPSQEDFDAFTSSKVADLAKPLKEAGFPHKIHIVKDHDMRERLCL 164

Query: 125 AIEDLKLDSLVMGSRGLSTIRR---ILLGSVSNYVITHSPCPVTVVK 158
             E L L +++MGSRG    +R     LGSVS+Y + H  CPV VV+
Sbjct: 165 ETERLNLSAVIMGSRGFGAEKRGSDGKLGSVSDYCVHHCVCPVVVVR 204

[Close]

BLAST of Lag0027203 vs. ExPASy Swiss-Prot
Match: P87132 (Uncharacterized protein C167.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC167.05 PE=1 SV=2)

HSP 1 Score: 59.7 bits (143), Expect = 3.6e-08
Identity = 46/149 (30.87%), Postives = 75/149 (50.34%), Query Frame = 0


Query: 9   VALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGSPLIPLSEFRE 68
           + LD S+ S +A +WA+  L   GDTL I+ V     D SA             + +  E
Sbjct: 435 LTLDLSSESLHAAEWAVGILLRNGDTLIIVDVIECD-DPSAR-----------AVKDRME 494

Query: 69  PDVLKNYDVKIDIEALDILDTGSRQKEITVVSKLYWGDAREKIIDAIEDLKLDSLVMGSR 128
            + L+  + KI    L +L     + E+  +  ++   A+  II+ I+ ++   +VMGSR
Sbjct: 495 SEQLETLE-KITKYILKLLSKTVLEVEVN-IEVIHHEKAKHLIIEMIDYIEPSLVVMGSR 554

Query: 129 GLSTIRRILLGSVSNYVITHSPCPVTVVK 158
           G S ++ +LLGS SNY++  S  PV V +
Sbjct: 555 GRSHLKGVLLGSFSNYLVNKSSVPVMVAR 569

[Close]

BLAST of Lag0027203 vs. ExPASy Swiss-Prot
Match: Q57951 (Universal stress protein MJ0531 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0531 PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.2e-06
Identity = 43/153 (28.10%), Postives = 72/153 (47.06%), Query Frame = 0


Query: 5   RKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGSPLIPLS 64
           +KI +  D S+ S  A K AI+   +    +Y IYV                 SP + L 
Sbjct: 27  KKIVIPTDGSDVSLEAAKHAINIAKEFDAEVYAIYV--------------VDVSPFVGLP 86

Query: 65  EFREPDVLKNYDVKIDIEALDILDTGSRQKEITVVSKLYWGDAREKIIDAIEDLKLDSLV 124
                +++     +   EAL  +   + +  + + +++  G    +I++  E  K D +V
Sbjct: 87  AEGSWELISELLKEEGQEALKKVKKMAEEWGVKIHTEMLEGVPANEIVEFAEKKKADLIV 146

Query: 125 MGSRGLSTIRRILLGSVSNYVITHSPCPVTVVK 158
           MG+ G + + RILLGSV+  VI ++ CPV VVK
Sbjct: 147 MGTTGKTGLERILLGSVAERVIKNAHCPVLVVK 165

[Close]

BLAST of Lag0027203 vs. ExPASy Swiss-Prot
Match: Q57997 (Universal stress protein MJ0577 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0577 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 9.8e-06
Identity = 31/76 (40.79%), Postives = 44/76 (57.89%), Query Frame = 0


Query: 85  DILDTGSRQKEITVVSKLYWGDAREKIIDAIEDLKLDSLVMGSRGLSTIRRILLGSVSNY 144
           ++ D G + K+I VV     G   E+I+   ED  +D ++MGS G + ++ ILLGSV+  
Sbjct: 91  ELEDVGFKVKDIIVV-----GIPHEEIVKIAEDEGVDIIIMGSHGKTNLKEILLGSVTEN 150

Query: 145 VITHSPCPVTVVKDSN 161
           VI  S  PV VVK  N
Sbjct: 151 VIKKSNKPVLVVKRKN 161

The following BLAST results are available for this feature:

BLAST of Lag0027203 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903504.1	2.8e-82	93.90	universal stress protein PHOS32 [Benincasa hispida]	[more]
XP_011651876.1	1.1e-81	93.29	universal stress protein PHOS32 [Cucumis sativus] >KGN58822.1 hypothetical prote...	[more]
XP_008442491.1	1.8e-81	93.29	PREDICTED: uncharacterized protein C167.05 [Cucumis melo] >KAA0044129.1 universa...	[more]
XP_022145652.1	2.9e-79	90.24	universal stress protein PHOS32 [Momordica charantia]	[more]
XP_022971337.1	2.7e-77	90.24	universal stress protein PHOS32-like [Cucurbita maxima]	[more]

BLAST of Lag0027203 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYN9	2.2e-13	31.90	Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 S...	[more]
Q8L4N1	7.0e-12	32.93	Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 S...	[more]
P87132	3.6e-08	30.87	Uncharacterized protein C167.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC ...	[more]
Q57951	1.2e-06	28.10	Universal stress protein MJ0531 OS=Methanocaldococcus jannaschii (strain ATCC 43...	[more]
Q57997	9.8e-06	40.79	Universal stress protein MJ0577 OS=Methanocaldococcus jannaschii (strain ATCC 43...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006015	Universal stress protein A family	PRINTS	PR01438	UNVRSLSTRESS	coord: 135..157 score: 45.22 coord: 4..22 score: 31.58 coord: 117..129 score: 37.85
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 1..163 e-value: 6.3E-36 score: 126.3
IPR006016	UspA	PFAM	PF00582	Usp	coord: 5..157 e-value: 2.0E-34 score: 119.1
None	No IPR available	PANTHER	PTHR46100:SF2	OS05G0453700 PROTEIN	coord: 4..163
None	No IPR available	PANTHER	PTHR46100	IMP2'P	coord: 4..163
None	No IPR available	CDD	cd00293	USP_Like	coord: 6..156 e-value: 3.06323E-31 score: 107.456
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 5..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0027203.1	Lag0027203.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0061077	chaperone-mediated protein folding
biological_process	GO:0009409	response to cold
biological_process	GO:0009408	response to heat
biological_process	GO:0006979	response to oxidative stress
biological_process	GO:0034337	RNA folding
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003676	nucleic acid binding