Lag0027176 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0027176
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	tropinone reductase homolog
Location	chr10: 45616110 .. 45617646 (-)
RNA-Seq Expression	Lag0027176
Synteny	Lag0027176

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCAGTGGGGACGCTGAACGGAGATCAACGATGGTCCCTCCCCGGGAAGACTGCTCCTGTCACCGACGAGACCCAGGGCATAGGGTTTGTTTATTATTCTTCTACACATCCACCATTTTAATTTTCTTTATAATTACTTATGTCCTATTTGATAACCGTTTTTTTTTTTTTTTTGACAACATGTGAGGGTGGGGGATTCAAACCATAGACCTTTTGGTCATTAGTATAAGACTTATGCTAGTTGAGTTATGTTCATGTTGGTTTATAAACATCTATTCATCAGTTTATTATTTAATCATCTACATTTTTTTTTCTATGTTCTCAAAAATTTAAGAATGTTTTGAAAATTAAAAAGAATGGTTTTCAAAAACTTATTTTAATTTTGTTTAGAATTCAAATGTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTTTAGAAGAATTCAAATGTATTCTTAACAAAAGATGAAAACTATTATTAGAAAATTAGAAGAAAAAAACATTATTTTAAAAAATCAAATGTCTATCAAATGGGATTTTAAAAATTCAAATGTGATGAAGTTAGGTTTAAAATGTATTTTGAGATTCTTTTTTTTTTATTTTAAAACTAATTTTCCTTATTATTTTGAAGTTAGTTTTTGTTTATTCGATAACAACAACAATAATTTTCATTCCAAAAGAGTACCATGTAAATATATTTATCAAATTTGTAGACAAAAAACCTGAATTTATTGAAAAGGACTGAAATTGAATATTTTATAATATGAAGATTAGAAAAGAAAAAAAAGTTACAAAGTATAAAAACTAAAAGCAGTATTTTTAATTATAGCTTTAATTTATTGTTGGAGATTATTTGGTTGCATACCGCTTAAGTATCTCCACACACACCATCAATACAAATTTCTCATTTTATACATTTCTCTCTCTTATTTGTACGATCACGTATTACTTTTTATTATTACATGACTAAATTGAAGTAATGCTGGCCAAATTTGTAATTGAACTTCCTTTTTAACTCCTTTTTTCTCTTCCATTTTCATTTACTTGTGTGTTGAAATAAAATAATGACACAGTGTGGAAGAACTAGCAGCAGTTGGAGCCAGAGTCCACACTTGTTCTCGTCACCAAGCAGAGATCGATCAATGTCTACAAGAATGGCGAAGCAAGGGCTTTCAAGTGACCGCCTCCGTCTGTGATAATGTTTCTGTTTCTCGTTTCTCGTTTTTCATTTCTCGTTACAATAGATATTAAGAAGTTTGTTTTGTTTTGTTTTCAATCTTAGGTTAATAATGCTGGGTTGAGTTTGTTCAAGAGCAGCACAACAGTTACAGATCAAGACATTTCATCAGTAATGGGTACAAATTTTGAGGCATCGTTTAATTTTTCCCAACTTGCCACACCCATTGCTCAAAGCATCAGAATCTGGAAGCATTGTGTTCATCTCTTCAGTTGCAGAGGTCACTTCACTTCCATTTGGTACTCTTTATGCTGCTTCTAA

mRNA sequence

ATGGCGGCAGTGGGGACGCTGAACGGAGATCAACGATGGTCCCTCCCCGGGAAGACTGCTCCTGTCACCGACGAGACCCAGGGCATAGGTGTGGAAGAACTAGCAGCAGTTGGAGCCAGAGTCCACACTTGTTCTCGTCACCAAGCAGAGATCGATCAATGTCTACAAGAATGGCGAAGCAAGGGCTTTCAAGTGACCGCCTCCGTCTGTGATAATGTTAATAATGCTGGGTTGAGTTTGTTCAAGAGCAGCACAACAGTTACAGATCAAGACATTTCATCAGTAATGGGTACAAATTTTGAGGCATCGTTTAATTTTTCCCAACTTGCCACACCCATTGCTCAAAGCATCAGAATCTGGAAGCATTGTGTTCATCTCTTCAGTTGCAGAGGTCACTTCACTTCCATTTGGTACTCTTTATGCTGCTTCTAA

Coding sequence (CDS)

ATGGCGGCAGTGGGGACGCTGAACGGAGATCAACGATGGTCCCTCCCCGGGAAGACTGCTCCTGTCACCGACGAGACCCAGGGCATAGGTGTGGAAGAACTAGCAGCAGTTGGAGCCAGAGTCCACACTTGTTCTCGTCACCAAGCAGAGATCGATCAATGTCTACAAGAATGGCGAAGCAAGGGCTTTCAAGTGACCGCCTCCGTCTGTGATAATGTTAATAATGCTGGGTTGAGTTTGTTCAAGAGCAGCACAACAGTTACAGATCAAGACATTTCATCAGTAATGGGTACAAATTTTGAGGCATCGTTTAATTTTTCCCAACTTGCCACACCCATTGCTCAAAGCATCAGAATCTGGAAGCATTGTGTTCATCTCTTCAGTTGCAGAGGTCACTTCACTTCCATTTGGTACTCTTTATGCTGCTTCTAA

Protein sequence

MAAVGTLNGDQRWSLPGKTAPVTDETQGIGVEELAAVGARVHTCSRHQAEIDQCLQEWRSKGFQVTASVCDNVNNAGLSLFKSSTTVTDQDISSVMGTNFEASFNFSQLATPIAQSIRIWKHCVHLFSCRGHFTSIWYSLCCF

Homology

[Close]

BLAST of Lag0027176 vs. NCBI nr
Match: XP_022159090.1 (tropinone reductase homolog At5g06060-like isoform X1 [Momordica charantia])

HSP 1 Score: 151.8 bits (382), Expect = 4.6e-33
Identity = 85/143 (59.44%), Postives = 99/143 (69.23%), Query Frame = 0


Query: 1   MAAVGTLNGDQRWSLPGKTAPVTDETQGIG---VEELAAVGARVHTCSRHQAEIDQCLQE 60
           MA VG L+ DQRWSL GK A VT  T+GIG   VEELAA+GA VHTCSR+Q E+DQ LQ+
Sbjct: 31  MAEVGMLSRDQRWSLTGKAALVTGGTRGIGRAIVEELAALGASVHTCSRNQVELDQILQQ 90

Query: 61  WRSKGFQVTASVCD------------------------NVNNAGLSLFKSSTTVTDQDIS 117
           WR++GFQV+ SVCD                         VNNAGLSLFKSST VT+QDIS
Sbjct: 91  WRNQGFQVSGSVCDVLVKEQREKLIETVSSLFGGKLNILVNNAGLSLFKSSTAVTEQDIS 150

[Close]

BLAST of Lag0027176 vs. NCBI nr
Match: XP_022159091.1 (senescence-associated protein 13-like isoform X2 [Momordica charantia])

HSP 1 Score: 151.8 bits (382), Expect = 4.6e-33
Identity = 85/143 (59.44%), Postives = 99/143 (69.23%), Query Frame = 0


Query: 1   MAAVGTLNGDQRWSLPGKTAPVTDETQGIG---VEELAAVGARVHTCSRHQAEIDQCLQE 60
           MA VG L+ DQRWSL GK A VT  T+GIG   VEELAA+GA VHTCSR+Q E+DQ LQ+
Sbjct: 31  MAEVGMLSRDQRWSLTGKAALVTGGTRGIGRAIVEELAALGASVHTCSRNQVELDQILQQ 90

Query: 61  WRSKGFQVTASVCD------------------------NVNNAGLSLFKSSTTVTDQDIS 117
           WR++GFQV+ SVCD                         VNNAGLSLFKSST VT+QDIS
Sbjct: 91  WRNQGFQVSGSVCDVLVKEQREKLIETVSSLFGGKLNILVNNAGLSLFKSSTAVTEQDIS 150

[Close]

BLAST of Lag0027176 vs. NCBI nr
Match: XP_015897157.1 (tropinone reductase homolog isoform X1 [Ziziphus jujuba])

HSP 1 Score: 125.9 bits (315), Expect = 2.7e-25
Identity = 66/136 (48.53%), Postives = 90/136 (66.18%), Query Frame = 0


Query: 8   NGDQRWSLPGKTAPVTDETQGIG---VEELAAVGARVHTCSRHQAEIDQCLQEWRSKGFQ 67
           +G+ RW+L GKTA VT  T+GIG   VEELA  GA +HTCSR+Q E+DQCL +W+SKGFQ
Sbjct: 8   SGENRWTLQGKTALVTGGTKGIGHAIVEELAGFGATIHTCSRNQNELDQCLGKWKSKGFQ 67

Query: 68  VTASVCD------------------------NVNNAGLSLFKSSTTVTDQDISSVMGTNF 117
           V+ SVCD                         VNNAG+++ KS+T VT QD S++MGTNF
Sbjct: 68  VSGSVCDVLYRDQREKLIETLSSLFEGKLNILVNNAGMTIPKSTTEVTAQDFSTIMGTNF 127

[Close]

BLAST of Lag0027176 vs. NCBI nr
Match: XP_022941371.1 (tropinone reductase homolog [Cucurbita moschata])

HSP 1 Score: 124.8 bits (312), Expect = 6.0e-25
Identity = 69/134 (51.49%), Postives = 84/134 (62.69%), Query Frame = 0


Query: 10  DQRWSLPGKTAPVTDETQGIG---VEELAAVGARVHTCSRHQAEIDQCLQEWRSKGFQVT 69
           DQRWSL G TA VT  T GIG   VEELAA GA VHTCSR Q ++D+CL+EW   GF V+
Sbjct: 7   DQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEEMGFNVS 66

Query: 70  ASVCD------------------------NVNNAGLSLFKSSTTVTDQDISSVMGTNFEA 117
            SVCD                         VNNAG S +KSS  VT++++SSVM TNFEA
Sbjct: 67  GSVCDVHSREQRMQLIETVSSLFNGSLNILVNNAGTSAYKSSLEVTEEELSSVMSTNFEA 126

[Close]

BLAST of Lag0027176 vs. NCBI nr
Match: KAF3449095.1 (hypothetical protein FNV43_RR09819 [Rhamnella rubrinervis])

HSP 1 Score: 124.0 bits (310), Expect = 1.0e-24
Identity = 67/134 (50.00%), Postives = 86/134 (64.18%), Query Frame = 0


Query: 10  DQRWSLPGKTAPVTDETQGIG---VEELAAVGARVHTCSRHQAEIDQCLQEWRSKGFQVT 69
           D RWSL G TA VT  T+GIG   VEELA  GA +HTCSR+QA++DQCL+EW+SKGF V+
Sbjct: 10  DTRWSLKGTTALVTGGTRGIGHAIVEELAGFGATIHTCSRNQAQLDQCLEEWKSKGFNVS 69

Query: 70  ASVCD------------------------NVNNAGLSLFKSSTTVTDQDISSVMGTNFEA 117
            SVCD                         VNNAG  L K++  VT Q++S++MGTNFE+
Sbjct: 70  GSVCDLLYWDQRQKLMGTVSSVFQGKLNILVNNAGTVLLKNTEDVTAQELSTIMGTNFES 129

[Close]

BLAST of Lag0027176 vs. ExPASy Swiss-Prot
Match: P50165 (Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 2.4e-24
Identity = 62/135 (45.93%), Postives = 82/135 (60.74%), Query Frame = 0


Query: 9   GDQRWSLPGKTAPVTDETQGIG---VEELAAVGARVHTCSRHQAEIDQCLQEWRSKGFQV 68
           GD+RWSL G TA VT  T+GIG   VEELA  GA V+TCSR Q ++D+CL++WR KGF+V
Sbjct: 9   GDRRWSLRGMTALVTGGTRGIGYAIVEELANFGAEVYTCSRSQNDLDECLEKWRRKGFKV 68

Query: 69  TASVCD------------------------NVNNAGLSLFKSSTTVTDQDISSVMGTNFE 117
           +  VCD                         +NNAG ++ K +T  T +D S +MGTNFE
Sbjct: 69  SGPVCDVSSISQRQTLMESVTSSFNGKLNILINNAGTTIPKEATNFTAEDYSIIMGTNFE 128

[Close]

BLAST of Lag0027176 vs. ExPASy Swiss-Prot
Match: Q9ZW18 (Senescence-associated protein 13 OS=Arabidopsis thaliana OX=3702 GN=SAG13 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.2e-23
Identity = 63/143 (44.06%), Postives = 85/143 (59.44%), Query Frame = 0


Query: 1   MAAVGTLNGDQRWSLPGKTAPVTDETQGIG---VEELAAVGARVHTCSRHQAEIDQCLQE 60
           MA  G L  + RWSL G TA VT  ++GIG   VEELA +GA+VHTC+R + ++ + L+E
Sbjct: 1   MAKEGGLGENSRWSLGGMTALVTGGSKGIGEAVVEELAMLGAKVHTCARDETQLQERLRE 60

Query: 61  WRSKGFQVTASVCD------------------------NVNNAGLSLFKSSTTVTDQDIS 117
           W++KGFQVT SVCD                         VNN G S+FK +T  T +D S
Sbjct: 61  WQAKGFQVTTSVCDVSSRDQRVKLMETVSSLYQGKLNILVNNVGTSIFKPTTEYTAEDFS 120

[Close]

BLAST of Lag0027176 vs. ExPASy Swiss-Prot
Match: Q9ZW04 (Tropinone reductase homolog At2g29170 OS=Arabidopsis thaliana OX=3702 GN=At2g29170 PE=3 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 8.5e-22
Identity = 56/132 (42.42%), Postives = 81/132 (61.36%), Query Frame = 0


Query: 12  RWSLPGKTAPVTDETQGIG---VEELAAVGARVHTCSRHQAEIDQCLQEWRSKGFQVTAS 71
           RWSL G TA VT  ++G+G   VEELA +GARVHTC+R++ ++ +C++EW++KGF+VT S
Sbjct: 13  RWSLGGMTALVTGGSKGLGEAVVEELAMLGARVHTCARNETQLQECVREWQAKGFEVTTS 72

Query: 72  VCD------------------------NVNNAGLSLFKSSTTVTDQDISSVMGTNFEASF 117
           VCD                         VNNAG  + K +T  T QD S +M TN +++F
Sbjct: 73  VCDVSSRDQREKLMENVASIFQGKLNILVNNAGTGITKPTTEYTAQDYSFLMATNLDSAF 132

[Close]

BLAST of Lag0027176 vs. ExPASy Swiss-Prot
Match: A7DY56 (Tropinone reductase OS=Cochlearia officinalis OX=270110 GN=TR PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.1e-21
Identity = 59/132 (44.70%), Postives = 78/132 (59.09%), Query Frame = 0


Query: 12  RWSLPGKTAPVTDETQGIG---VEELAAVGARVHTCSRHQAEIDQCLQEWRSKGFQVTAS 71
           RWSL G TA VT  ++GIG   VEELA +GARVHTC+R + ++ + L+EW++KGFQVT S
Sbjct: 13  RWSLEGMTALVTGGSKGIGEAVVEELAMLGARVHTCARDETQLQESLREWQAKGFQVTTS 72

Query: 72  VCD------------------------NVNNAGLSLFKSSTTVTDQDISSVMGTNFEASF 117
           VCD                         VNNAG  + K +   T +D S +M TN E+SF
Sbjct: 73  VCDVSSRDQREKLMETVSSLFQGKLNILVNNAGTCITKPTIDYTSEDFSFLMSTNLESSF 132

[Close]

BLAST of Lag0027176 vs. ExPASy Swiss-Prot
Match: Q9ZW03 (Tropinone reductase homolog At2g29150 OS=Arabidopsis thaliana OX=3702 GN=At2g29150 PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 1.9e-21
Identity = 60/144 (41.67%), Postives = 85/144 (59.03%), Query Frame = 0


Query: 1   MAAVGTLNGDQ-RWSLPGKTAPVTDETQGIG---VEELAAVGARVHTCSRHQAEIDQCLQ 60
           MA  G  + D+ RWSL G TA VT  ++G+G   VEELA +GARVHTC+R + ++ + L+
Sbjct: 1   MAKAGENSRDKSRWSLEGMTALVTGGSKGLGEAVVEELAMLGARVHTCARDETQLQERLR 60

Query: 61  EWRSKGFQVTASVCD------------------------NVNNAGLSLFKSSTTVTDQDI 117
           EW++KGF+VT SVCD                         VNNAG  + K ST  T +D 
Sbjct: 61  EWQAKGFEVTTSVCDVSSREQREKLMETVSSVFQGKLNILVNNAGTGIIKPSTEYTAEDY 120

The following BLAST results are available for this feature:

BLAST of Lag0027176 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159090.1	4.6e-33	59.44	tropinone reductase homolog At5g06060-like isoform X1 [Momordica charantia]	[more]
XP_022159091.1	4.6e-33	59.44	senescence-associated protein 13-like isoform X2 [Momordica charantia]	[more]
XP_015897157.1	2.7e-25	48.53	tropinone reductase homolog isoform X1 [Ziziphus jujuba]	[more]
XP_022941371.1	6.0e-25	51.49	tropinone reductase homolog [Cucurbita moschata]	[more]
KAF3449095.1	1.0e-24	50.00	hypothetical protein FNV43_RR09819 [Rhamnella rubrinervis]	[more]

BLAST of Lag0027176 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P50165	2.4e-24	45.93	Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1	[more]
Q9ZW18	1.2e-23	44.06	Senescence-associated protein 13 OS=Arabidopsis thaliana OX=3702 GN=SAG13 PE=1 S...	[more]
Q9ZW04	8.5e-22	42.42	Tropinone reductase homolog At2g29170 OS=Arabidopsis thaliana OX=3702 GN=At2g291...	[more]
A7DY56	1.1e-21	44.70	Tropinone reductase OS=Cochlearia officinalis OX=270110 GN=TR PE=1 SV=1	[more]
Q9ZW03	1.9e-21	41.67	Tropinone reductase homolog At2g29150 OS=Arabidopsis thaliana OX=3702 GN=At2g291...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.720		coord: 8..72 e-value: 2.2E-7 score: 32.2
None	No IPR available	GENE3D	3.40.50.720		coord: 73..140 e-value: 3.4E-5 score: 25.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
IPR045000	Tropinone reductase	PANTHER	PTHR42898	TROPINONE REDUCTASE	coord: 73..129 coord: 10..71
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 13..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0027176.1	Lag0027176.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity