Lag0027142 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0027142
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	RING-type E3 ubiquitin transferase
Location	chr10: 45381591 .. 45382145 (+)
RNA-Seq Expression	Lag0027142
Synteny	Lag0027142

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACACCAGCGCCACCGTGACGAATCGACCAACGCCGCCGTACCCGTCGCCGCATCGGACCGTTGATTTCTCTCCGATCGAGTTCATCCTCGGCCTCGTCGCCGTCATCACCATCCCCGCCCTTGTCTACTCCTTCTTCTTCGCCATCAAGTGCCCGCCCAATCTCCTCCGTCTCTGGCGGCGGAGATCCGCCAGGCTCTCCGGCGCCGCCGTCACCGTGGTGGCTGGGAATCCTACTTCTTCTAGTGACGTGGCGGCGGCTGTAAAGTACAACAAGGACGAGCACTGTAAGGAAGTCGGCAACGAGTGTCCGGTGTGCCTGTCGGCGTTCGCCGACGGCGAGGAGGTCCGGCAACTCGCCGCCTGTAAGCACTCTTTTCATGTGGATTGCATCGATATGTGGCTCTATTCTCACCCTAATTGCCCTGTTTGTCGGGCCGCCGTCGCCGTCGCCGTTAAACGGCCGATTGACAACCGTGCGCCGTCGCGGCCGGCGGCTTCTAGAAACGACGATTACCAGCAAGGTCTGCCAGATGCCGGAAATTTAGTTTGA

mRNA sequence

ATGGACACCAGCGCCACCGTGACGAATCGACCAACGCCGCCGTACCCGTCGCCGCATCGGACCGTTGATTTCTCTCCGATCGAGTTCATCCTCGGCCTCGTCGCCGTCATCACCATCCCCGCCCTTGTCTACTCCTTCTTCTTCGCCATCAAGTGCCCGCCCAATCTCCTCCGTCTCTGGCGGCGGAGATCCGCCAGGCTCTCCGGCGCCGCCGTCACCGTGGTGGCTGGGAATCCTACTTCTTCTAGTGACGTGGCGGCGGCTGTAAAGTACAACAAGGACGAGCACTGTAAGGAAGTCGGCAACGAGTGTCCGGTGTGCCTGTCGGCGTTCGCCGACGGCGAGGAGGTCCGGCAACTCGCCGCCTGTAAGCACTCTTTTCATGTGGATTGCATCGATATGTGGCTCTATTCTCACCCTAATTGCCCTGTTTGTCGGGCCGCCGTCGCCGTCGCCGTTAAACGGCCGATTGACAACCGTGCGCCGTCGCGGCCGGCGGCTTCTAGAAACGACGATTACCAGCAAGGTCTGCCAGATGCCGGAAATTTAGTTTGA

Coding sequence (CDS)

ATGGACACCAGCGCCACCGTGACGAATCGACCAACGCCGCCGTACCCGTCGCCGCATCGGACCGTTGATTTCTCTCCGATCGAGTTCATCCTCGGCCTCGTCGCCGTCATCACCATCCCCGCCCTTGTCTACTCCTTCTTCTTCGCCATCAAGTGCCCGCCCAATCTCCTCCGTCTCTGGCGGCGGAGATCCGCCAGGCTCTCCGGCGCCGCCGTCACCGTGGTGGCTGGGAATCCTACTTCTTCTAGTGACGTGGCGGCGGCTGTAAAGTACAACAAGGACGAGCACTGTAAGGAAGTCGGCAACGAGTGTCCGGTGTGCCTGTCGGCGTTCGCCGACGGCGAGGAGGTCCGGCAACTCGCCGCCTGTAAGCACTCTTTTCATGTGGATTGCATCGATATGTGGCTCTATTCTCACCCTAATTGCCCTGTTTGTCGGGCCGCCGTCGCCGTCGCCGTTAAACGGCCGATTGACAACCGTGCGCCGTCGCGGCCGGCGGCTTCTAGAAACGACGATTACCAGCAAGGTCTGCCAGATGCCGGAAATTTAGTTTGA

Protein sequence

MDTSATVTNRPTPPYPSPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLWRRRSARLSGAAVTVVAGNPTSSSDVAAAVKYNKDEHCKEVGNECPVCLSAFADGEEVRQLAACKHSFHVDCIDMWLYSHPNCPVCRAAVAVAVKRPIDNRAPSRPAASRNDDYQQGLPDAGNLV

Homology

[Close]

BLAST of Lag0027142 vs. NCBI nr
Match: XP_038905078.1 (RING-H2 finger protein ATL33 [Benincasa hispida])

HSP 1 Score: 327.8 bits (839), Expect = 6.0e-86
Identity = 160/184 (86.96%), Postives = 167/184 (90.76%), Query Frame = 0


Query: 1   MDTSATVTNRPTPPYPSPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60
           MD SA++ NR  PP P+PHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW
Sbjct: 1   MDKSASLMNRSPPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60

Query: 61  RRRSARLSGAAVTVVAGNPTSSSDVAAAVKYNKDEHCKEVGNECPVCLSAFADGEEVRQL 120
           R RSARLSGAAVTVV  N T SSDVAAAVKYNKDEHCKEV NECPVCLSAF DGEE+RQL
Sbjct: 61  RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL 120

Query: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVAVAVKRPIDNRAPSRPAASRNDDYQQGLPDA 180
           AACKHSFHVDCIDMWLYSHPNCPVCRAAV VAVKRPI+N AP RPA SR+DD+QQGLPDA
Sbjct: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDA 180

Query: 181 GNLV 185
           G LV
Sbjct: 181 GYLV 184

[Close]

BLAST of Lag0027142 vs. NCBI nr
Match: KAE8651013.1 (hypothetical protein Csa_001104 [Cucumis sativus])

HSP 1 Score: 322.4 bits (825), Expect = 2.5e-84
Identity = 155/184 (84.24%), Postives = 162/184 (88.04%), Query Frame = 0


Query: 1   MDTSATVTNRPTPPYPSPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60
           MD +A+  NR  PPYP+PHRTVDFSPIEFILGLVAVITIPALVYSF FAIKCPPN LRLW
Sbjct: 42  MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW 101

Query: 61  RRRSARLSGAAVTVVAGNPTSSSDVAAAVKYNKDEHCKEVGNECPVCLSAFADGEEVRQL 120
           R+RS RLSGA  TVV GN T SSDVAAAVKYNKDEHCKEVGNECPVCLSAF DGEE+RQL
Sbjct: 102 RQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQL 161

Query: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVAVAVKRPIDNRAPSRPAASRNDDYQQGLPDA 180
           A CKHSFHVDCIDMWLYSHPNCPVCRAAV V VKRPI N AP RPAASR+DD+ QGLPDA
Sbjct: 162 AVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDA 221

Query: 181 GNLV 185
           G LV
Sbjct: 222 GYLV 225

[Close]

BLAST of Lag0027142 vs. NCBI nr
Match: XP_004137943.1 (RING-H2 finger protein ATL33 [Cucumis sativus])

HSP 1 Score: 322.4 bits (825), Expect = 2.5e-84
Identity = 155/184 (84.24%), Postives = 162/184 (88.04%), Query Frame = 0


Query: 1   MDTSATVTNRPTPPYPSPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60
           MD +A+  NR  PPYP+PHRTVDFSPIEFILGLVAVITIPALVYSF FAIKCPPN LRLW
Sbjct: 1   MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW 60

Query: 61  RRRSARLSGAAVTVVAGNPTSSSDVAAAVKYNKDEHCKEVGNECPVCLSAFADGEEVRQL 120
           R+RS RLSGA  TVV GN T SSDVAAAVKYNKDEHCKEVGNECPVCLSAF DGEE+RQL
Sbjct: 61  RQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQL 120

Query: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVAVAVKRPIDNRAPSRPAASRNDDYQQGLPDA 180
           A CKHSFHVDCIDMWLYSHPNCPVCRAAV V VKRPI N AP RPAASR+DD+ QGLPDA
Sbjct: 121 AVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDA 180

Query: 181 GNLV 185
           G LV
Sbjct: 181 GYLV 184

[Close]

BLAST of Lag0027142 vs. NCBI nr
Match: XP_023540894.1 (RING-H2 finger protein ATL33-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 320.5 bits (820), Expect = 9.7e-84
Identity = 159/184 (86.41%), Postives = 166/184 (90.22%), Query Frame = 0


Query: 1   MDTSATVTNRPTPPYPSPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60
           MDTS TV NRPTPP+PSP RTV FSPIEFIL LVAVI IPALVYSFF AIKC PNLLRLW
Sbjct: 1   MDTSTTVMNRPTPPFPSPRRTVAFSPIEFILALVAVIIIPALVYSFFIAIKCSPNLLRLW 60

Query: 61  RRRSARLSGAAVTVVAGNPTSSSDVAAAVKYNKDEHCKEVGNECPVCLSAFADGEEVRQL 120
            RRSARL GAAVTVVA N   S+DVAAAV YNKD+HCKEVGNECPVCLSAFADGE+VRQL
Sbjct: 61  PRRSARLPGAAVTVVAVN-RISNDVAAAVVYNKDQHCKEVGNECPVCLSAFADGEKVRQL 120

Query: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVAVAVKRPIDNRAPSRPAASRNDDYQQGLPDA 180
           AACKHSFHVDCIDMWLYSHPNCPVCRA V +AVKRPI+NRAP RPAASRN+DYQQGLPDA
Sbjct: 121 AACKHSFHVDCIDMWLYSHPNCPVCRATVTIAVKRPINNRAPPRPAASRNNDYQQGLPDA 180

Query: 181 GNLV 185
           GNLV
Sbjct: 181 GNLV 183

[Close]

BLAST of Lag0027142 vs. NCBI nr
Match: KAG6596414.1 (RING-H2 finger protein ATL33, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027958.1 RING-H2 finger protein ATL33, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 320.1 bits (819), Expect = 1.3e-83
Identity = 160/184 (86.96%), Postives = 165/184 (89.67%), Query Frame = 0


Query: 1   MDTSATVTNRPTPPYPSPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60
           MD S TV NRPTPP+PSP RTV FSPIEFIL LVAVI IPALVYSFF AIKC PNLLRLW
Sbjct: 1   MDASTTVMNRPTPPFPSPRRTVAFSPIEFILALVAVIIIPALVYSFFIAIKCSPNLLRLW 60

Query: 61  RRRSARLSGAAVTVVAGNPTSSSDVAAAVKYNKDEHCKEVGNECPVCLSAFADGEEVRQL 120
            RRSARLSG AVTVVA N   S+DVAAAVKYNKDEH KEVGNECPVCLSAF DGE+VRQL
Sbjct: 61  PRRSARLSGTAVTVVAVN-RISNDVAAAVKYNKDEHGKEVGNECPVCLSAFVDGEKVRQL 120

Query: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVAVAVKRPIDNRAPSRPAASRNDDYQQGLPDA 180
           AACKHSFHVDCIDMWLYSHPNCPVCRA VA+AVKRPI+NRAP RPAASRNDDYQQGLPDA
Sbjct: 121 AACKHSFHVDCIDMWLYSHPNCPVCRATVAIAVKRPINNRAPPRPAASRNDDYQQGLPDA 180

Query: 181 GNLV 185
           GNLV
Sbjct: 181 GNLV 183

[Close]

BLAST of Lag0027142 vs. ExPASy Swiss-Prot
Match: O80927 (RING-H2 finger protein ATL33 OS=Arabidopsis thaliana OX=3702 GN=ATL33 PE=2 SV=2)

HSP 1 Score: 145.6 bits (366), Expect = 5.6e-34
Identity = 77/192 (40.10%), Postives = 108/192 (56.25%), Query Frame = 0


Query: 11  PTPPYPSPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLWRRRSARLSGA 70
           P PP+P+P R++D +P++ I  ++A + +PALVY+ FF   C  +       R++  S  
Sbjct: 46  PPPPFPAPPRSIDLTPLKLIFVVIAFVAVPALVYALFFNGPCSSSRRNSSSSRTSSSSDD 105

Query: 71  AVTVVAGNPTSSSDVAAAV---KYNKDEHCKEVGNECPVCLSAFADGEEVRQLAACKHSF 130
                   P  +     +    K++KD H KE+GNEC VCL  F D +E+RQL+ CKH+F
Sbjct: 106 TPHATVDTPPITETTVTSESGGKFHKDTHSKEIGNECSVCLMVFTDSDELRQLSECKHAF 165

Query: 131 HVDCIDMWLYSHPNCPVCRAAVAVA---------------VKRPIDNRAPSRPAASRNDD 185
           HV CI+ WL  HPNCP+CR  V+V                V R   NR  S  A SR+DD
Sbjct: 166 HVLCIETWLKDHPNCPICRTDVSVKQQTEAPNVPVNVNGNVNRSGGNRRVS--ATSRDDD 225

[Close]

BLAST of Lag0027142 vs. ExPASy Swiss-Prot
Match: Q9LRB7 (E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5.1 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.2e-13
Identity = 40/87 (45.98%), Postives = 49/87 (56.32%), Query Frame = 0


Query: 81  SSSDVAAAVKYNKDEHCKEVGNECPVCLSAFADGEEVRQLAACKHSFHVDCIDMWLYSHP 140
           S S  AAA K  ++E   + G EC VCL+   DGEE R L  C H FH +C+DMWL SH 
Sbjct: 113 SRSTAAAAAKEEEEE--DDDGVECAVCLAELEDGEEARFLPRCGHGFHAECVDMWLGSHS 172

Query: 141 NCPVCRAAVAVAVKRPIDNRAPSRPAA 168
            CP+CR  V V    P+    P  PA+
Sbjct: 173 TCPLCRLTVVVP-PPPLPPVPPEPPAS 196

[Close]

BLAST of Lag0027142 vs. ExPASy Swiss-Prot
Match: Q9XF63 (RING-H2 finger protein ATL3 OS=Arabidopsis thaliana OX=3702 GN=ATL3 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.4e-13
Identity = 39/97 (40.21%), Postives = 52/97 (53.61%), Query Frame = 0


Query: 53  PPNLLRLWRRRSARLSGAAVTVVAGNPTSSSDVAAAVKYNKDEHCKEVGNECPVCLSAFA 112
           PP + R  RRR   + G       G  TS    +  + + + + CK+ G EC +CLS   
Sbjct: 78  PPVVTRRQRRRFIFVPGQDALSNTG-LTSFELSSLPIVFFRQDSCKD-GLECSICLSELV 137

Query: 113 DGEEVRQLAACKHSFHVDCIDMWLYSHPNCPVCRAAV 150
            G++ R L  C HSFHV+CIDMW  SH  CP+CR  V
Sbjct: 138 KGDKARLLPKCNHSFHVECIDMWFQSHSTCPICRNTV 172

[Close]

BLAST of Lag0027142 vs. ExPASy Swiss-Prot
Match: Q9SJJ7 (RING-H2 finger protein ATL57 OS=Arabidopsis thaliana OX=3702 GN=ATL57 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.6e-12
Identity = 54/186 (29.03%), Postives = 85/186 (45.70%), Query Frame = 0


Query: 3   TSATVTNRPTPPYPSPHRTVDFSPIEFILGLVAVITIPALVYSFFFAI---------KCP 62
           T   VT+ P  P P P      S ++  + L   I + AL +  FF++            
Sbjct: 25  TVQNVTSSPLTPTPPPQPP---SALDSTMALTIFILLVALFFMGFFSVYFRHFADSSSST 84

Query: 63  PNLLRLWRRRSARLS--GAAVTVVAGNPTS------SSDVAAAVKYNKDEHCKEVGNECP 122
            ++  + R RS+R+S    + +VV   P S      S  V +   Y   +  K+   +C 
Sbjct: 85  VDISSMPRTRSSRMSPRRLSTSVVVSRPYSFRRGLDSQAVRSLPVYRYTKAAKQRNEDCV 144

Query: 123 VCLSAFADGEEVRQLAACKHSFHVDCIDMWLYSHPNCPVCRAAVAVAVKRPIDNRAPSRP 172
           +CLS F +GE V+ +  C H FHVDC+D WL S+  CP+CR+    + K       P + 
Sbjct: 145 ICLSDFEEGETVKVIPHCGHVFHVDCVDTWLSSYVTCPLCRSNQLFSDKDLGMQEPPDQD 204

[Close]

BLAST of Lag0027142 vs. ExPASy Swiss-Prot
Match: Q8LFY8 (RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 2.1e-12
Identity = 27/49 (55.10%), Postives = 36/49 (73.47%), Query Frame = 0


Query: 103 ECPVCLSAFADGEEVRQLAACKHSFHVDCIDMWLYSHPNCPVCRAAVAV 152
           +CPVCL+ F + E +R L  C H+FH+ CID WL SH NCP+CRA +A+
Sbjct: 176 DCPVCLNEFEEDESLRLLPKCNHAFHISCIDTWLSSHTNCPLCRAGIAM 224

The following BLAST results are available for this feature:

BLAST of Lag0027142 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905078.1	6.0e-86	86.96	RING-H2 finger protein ATL33 [Benincasa hispida]	[more]
KAE8651013.1	2.5e-84	84.24	hypothetical protein Csa_001104 [Cucumis sativus]	[more]
XP_004137943.1	2.5e-84	84.24	RING-H2 finger protein ATL33 [Cucumis sativus]	[more]
XP_023540894.1	9.7e-84	86.41	RING-H2 finger protein ATL33-like [Cucurbita pepo subsp. pepo]	[more]
KAG6596414.1	1.3e-83	86.96	RING-H2 finger protein ATL33, partial [Cucurbita argyrosperma subsp. sororia] >K...	[more]

BLAST of Lag0027142 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80927	5.6e-34	40.10	RING-H2 finger protein ATL33 OS=Arabidopsis thaliana OX=3702 GN=ATL33 PE=2 SV=2	[more]
Q9LRB7	3.2e-13	45.98	E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5....	[more]
Q9XF63	5.4e-13	40.21	RING-H2 finger protein ATL3 OS=Arabidopsis thaliana OX=3702 GN=ATL3 PE=2 SV=1	[more]
Q9SJJ7	1.6e-12	29.03	RING-H2 finger protein ATL57 OS=Arabidopsis thaliana OX=3702 GN=ATL57 PE=2 SV=1	[more]
Q8LFY8	2.1e-12	55.10	RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 104..145 e-value: 2.9E-6 score: 36.8
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 102..146 e-value: 5.7E-14 score: 52.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 104..146 score: 12.297489
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 73..151 e-value: 1.4E-20 score: 74.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 160..184
None	No IPR available	PANTHER	PTHR45676:SF88	RING-H2 FINGER PROTEIN ATL33	coord: 8..184
None	No IPR available	CDD	cd16461	RING-H2_EL5_like	coord: 103..146 e-value: 1.05613E-16 score: 68.1055
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 95..152
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 8..184

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0027142.1	Lag0027142.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane