Lag0026713 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0026713
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Ribonuclease H
Location	chr10: 40853211 .. 40857281 (+)
RNA-Seq Expression	Lag0026713
Synteny	Lag0026713

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGCTTCAACTTGTCGTGGGTGTCGACGATATGAATTGGTATCCGCCTAGTAGATTGGGGCACAGTGAGGGGTGGGACGAAGGTAAGAGGTAACCGACCAATGATGTTTTGGAAGTAAGCAACCTGAAAACATGTATAATTTCTTTTTTTTTTTTATCTGCCTTGTTGTTGTAGTTTGTTCCTCTTCTGATTTACCGTTTGTTAATTGGTTTTGTTTGTAGGTTGGTTTTTTCTTTTGTGTTCATGCTGTTAAAATTTGTATGACTTCATGTTTAATTTCTTTTGTGTTCATGCTGTTGTGTAAATTTCATTCCTCTCATGACCCCCCCCATTCGTATTTTGTGTTCATGCCGTTGTGTAAATTTGATTCCTCTCATGACCCCCCCCAATTCGTATTATGTGTTCATGTTGTTGTGTAATTTGTGTTGCATTCTTGTTTTTCCTTGTCGTTTTTTTCATGGTCTGATATTCCGTCTTATTTACCCTGTTATGCGCACATAATTTGAGTTAAGTTTGTTTGTTGGACACATTATTTGGTTTGATTTTGTCTTCTTTTGCCCACAATATTTAATTCAAGTTTGTGTTCTTCATTTTCAAGCCAGTGTTCAATAGACTGTGTTTTTTCCACTTCTTTCGTATTTTATACTTGTTTTGTCCGTTTCTTTTTTGTCATATTTGTTTGACTTCCATGGTCTTTTTTGTTCCTTCATGTTAATTTCATGTTGTTTTCCAAATTTTGAAATGTTTTCTACATTGAACAAAACCATGGTTGAAAAAAAAATTCCAACTGAGGCCGGTTACATTTTTTTCAGTATTTAAATATTTTTTTGTTCTTAGTTTCTGATATTTGGTTACTTTCTGACTAATCACATTGTGGTTGAACCAATGTTTTGTAAATCAAGTTTATTTGTGATGAGAAAAGTCTGATGTTGCATTTATTTCACCTTACTCACCATATCTTTCCATTTAAAATATTAGACTTAGTTGCATTTAGGTTGACTTTATAAATGCAACCAACCTTCTGAGTTAATAAATAAAGGCAGCCGGCATTTATATTTCGTGGTTTAATTATGCAACCACTACTTGTACTTTCATTCAACTACCTCCTTCAATCCTTTCCTTATTTAAAGAAGACCAACCATTTCTAATTTCTTTGACGAAAGTCTCCACTGCATTGGATAGTCCCTCTAAACGGTGAGTCATTTTCTAACTTGCATACTTTTCTTTCTTTGTGTTGCTATGTATACCAAGTTCTTCCTTTCACTAACTACAATCTCTTCTTATCTTCTGCCCTTAAGACAAATCTAAACCCGATCTCCACCTTCTTCTGTTTCGTTTCACGCATCCCGATCTCGTATAACGGTAAGTTTTCATTCTCTTCCTTCGTTCATTTCGTTTCTACTTGGTCTACATTCATACGGTCATATCTTAATAACATTCTCCCTTTATGTGATATGCTTGCAGGTTTACTCCAAAGAACACCGTTATATTCGAGGTTCGTTCGTTCGTTCAATTTAAATACCCTTATACTGTTCTGAAAATTGTGTATATTATTTGTTGTGACATTAGAAACTGCTTCATTTGTATTGTTTAAGTTCGGACATTTCATTGGTTTTTTTCTGTTTTTTCACGTTGTATTGTTTAAATACCCTTATACTATTCTGAAAATTGTGTATATTATTTGTTGTGGACATTAGAAACTGCTTCATTTGTATTGTTTAAGTTCGGACATTTCGTTGGTTTTTTTTTTTTCTGTTTTTTCACGTTGTATTGTTCATTGTGTTACTATTTAGCTTATGTAATGATACCATTTTCTGTATAAATGAAAATTTTCTTATGAAATTGACATATATTTTAAATAAATGGACGTAAAAAATATGTTACGTTGTTTGAACGAATATTTAGTTGCTAAAATTCATCATGGTTTTACTATCATTTTCTATTTTGAATGTTGAAAAAAATATGCAGTAGCAATTTTGTTCACTAAAATATTGAAACAAAGAAAAAAAAATGGAATTTGAAGTTTACTATAACACAAAATTCAATACTATGAACATTTGCTTTAAAGAAGTTAAAATTTGTTTGACTCCATCTTCCTTGCTTCTAGCATTTTAGTTTTAAAAAAATGGAGACCTCACTATAATATGAAAAAGGTTCGGTTTTTTTATTTTTATTTTTTTTATTTTTTTATTTTTTATCTACAACACTTTGGTGAAAAATTTATTTCAGGTGGATAAATCATATTAAAAGAAATTTAATTTGGATTTCAGTTTTCATTCAAGTTTTTAAACCCATAGATTAATATCATATTCGTACTAACAAGTGAAAAAGGAATGAAATTATAAATTAACCACATTCATGTAATTAAAATGAATAAAAGTTATTTCATAAAAAAAGAAATAAACAACATACATCATGTTAAAGTAATATGTTTTATTTTATTATTATTTTTTTTTTACAAAATTGAAGTCATAAAAATAACATTTTCATGTGATTTCAAAAAAAGCCACGGAATGAACTGTTGAGTACATTTTTTTCCTCCAATTAGAGTTATTACGTTCTAAATTTTAGGAAAATAAATTTTATAACTTCCGTGAGTTAAGAAGTACAATATCCAAGTCAAATGATTTTTTTTTTTCCATTAGAGTTAATAATAGCAAAATTAAGGTAAAACAGATTTCCTAATTTGTGTTACAGGTAAGAAGGTTAAATTTGAAGTAAAATACACTCCCTAATTACATTTTGAGTTAAAGAAGTTTAAAATTGAAGTAATCAAAATATCATGTTTTGTCATTGAAGAGCATTTCATATTATTATCTTTATTAAAGTGTTTTTTATATACAATTATTTTCATTTTTCATGAGTTTAATTGCACAAAGAATTTCAAATAACAAGTATTGAAAATGTTACTATTATATATATATGCTCAATTTATATCTTATCACATCCTTTGATTTTCATTAAGCTTACTTTACTTTAATTGTACTTTGATACTTGTCTATGAGCTAATCTATCTTAGTTTAGTCTAGGGGTTATTGTGCATTTCGAGCGTATACTTCTAAACCCCAAAAGATAGATTCAATTATTTGGTAAAATAATACACAATAATTGTTTTGCGACGAAAGAACCAATTATAGAAATTACCTTTTCACCCAAAACCTCAAGTCATAAACCCTAAACTTACTAAACCCTAAATCGTGAACCCTAAACCCTAAACCACTAAACCACTAAACCCTAAACCACTCAACCTTAAACCCTAAACCCTAAACCAGTTAACCACTAAACCGTAAACTAGTAAACCCTAAACCACTTAACCACTTAACCACTAAACCATAAACCACTCAACCTTAAACCCTAAACCCTAAACCACTTAACCACTAAACCCTAAACCCTAAACCACTCAACCTTAACCCGAAACCCTAAACCACTTAACCACTAAACCGTAAACCAGTAAACCCTAACCCCTAAACCACTTAATCATTGAACACGAAACCGAAAAGTTTGTAACCTAAATCACGAACCTCAAACTTACCGAACCCTAAAACCTAAACACTAAACCCTATACCCTAAAGATTAAACCCTAAATCGTTGACCCTAAAACCGGAAACCATAAACCCTAATACCATGTACCCTAAAACCACTCGGACTAAAATCCGGATTCTTGTAATCATAAAACGAAATAACACGCTTTAAATATAGACAATAGGTGACTGCTTGACTTCGTACATTTTGAATAATAGGACTATGAGGGCAACAAAATTCTATGTCGTTTTTGTTGGTCGCAACCCAGGAATTTACACCTTGTGGGTTGAATGCCACAGACAAGTAAACCAGTTTAAGGGAGCACTACACAAGTCATATCCAACATTTCAAGAAGCAGAGTATGCATTTAGGCAGTACGTTGCGGGCACCCGGGGCGAAGCAAACCTCGTTGACCAACATGATGTGTGTCGTAACCTTCCTGTAGGGGGCTATGGAACCGTAGGAAAATGTTACAGTATTAGTCGGATGTTTGTAGGGTTCCTGTTCGGAATAATTGTAACTTACGTCATCGTTCACTATCTTTAG

mRNA sequence

ATGCCGCTTCAACTTGTCGTGGGTGTCGACGATATGAATTGGTATCCGCCTAGTAGATTGGGGCACAGTGAGGGGTGGGACGAAGGTTTACTCCAAAGAACACCGTTATATTCGAGGACTATGAGGGCAACAAAATTCTATGTCGTTTTTGTTGGTCGCAACCCAGGAATTTACACCTTGTGGGTTGAATGCCACAGACAAGTAAACCAGTTTAAGGGAGCACTACACAAGTCATATCCAACATTTCAAGAAGCAGAGTATGCATTTAGGCAGTACGTTGCGGGCACCCGGGGCGAAGCAAACCTCGTTGACCAACATGATGTGTGTCGTAACCTTCCTGTAGGGGGCTATGGAACCGTAGGAAAATGTTACAGTATTAGTCGGATGTTTGTAGGGTTCCTGTTCGGAATAATTGTAACTTACGTCATCGTTCACTATCTTTAG

Coding sequence (CDS)

ATGCCGCTTCAACTTGTCGTGGGTGTCGACGATATGAATTGGTATCCGCCTAGTAGATTGGGGCACAGTGAGGGGTGGGACGAAGGTTTACTCCAAAGAACACCGTTATATTCGAGGACTATGAGGGCAACAAAATTCTATGTCGTTTTTGTTGGTCGCAACCCAGGAATTTACACCTTGTGGGTTGAATGCCACAGACAAGTAAACCAGTTTAAGGGAGCACTACACAAGTCATATCCAACATTTCAAGAAGCAGAGTATGCATTTAGGCAGTACGTTGCGGGCACCCGGGGCGAAGCAAACCTCGTTGACCAACATGATGTGTGTCGTAACCTTCCTGTAGGGGGCTATGGAACCGTAGGAAAATGTTACAGTATTAGTCGGATGTTTGTAGGGTTCCTGTTCGGAATAATTGTAACTTACGTCATCGTTCACTATCTTTAG

Protein sequence

MPLQLVVGVDDMNWYPPSRLGHSEGWDEGLLQRTPLYSRTMRATKFYVVFVGRNPGIYTLWVECHRQVNQFKGALHKSYPTFQEAEYAFRQYVAGTRGEANLVDQHDVCRNLPVGGYGTVGKCYSISRMFVGFLFGIIVTYVIVHYL

Homology

[Close]

BLAST of Lag0026713 vs. NCBI nr
Match: RYR74402.1 (hypothetical protein Ahy_A02g009104 [Arachis hypogaea])

HSP 1 Score: 77.4 bits (189), Expect = 1.1e-10
Identity = 33/60 (55.00%), Postives = 42/60 (70.00%), Query Frame = 0


Query: 45  KFYVVFVGRNPGIYTLWVECHRQVNQFKGALHKSYPTFQEAEYAFRQYVAGTRGEANLVD 104
           K+YVVF+GR PGIY+ W  CH QVN+F G LHKSY TF EA  AF Q+++  R +  L +
Sbjct: 307 KYYVVFLGRVPGIYSSWPACHSQVNEFSGNLHKSYKTFDEARMAFEQFLSARRLQTQLTN 366

[Close]

BLAST of Lag0026713 vs. NCBI nr
Match: WP_138879431.1 (ribonuclease H family protein [Paenibacillus mesophilus] >TMV50118.1 ribonuclease H [Paenibacillus mesophilus])

HSP 1 Score: 70.5 bits (171), Expect = 1.4e-08
Identity = 33/61 (54.10%), Postives = 43/61 (70.49%), Query Frame = 0


Query: 41  MRATKFYVVFVGRNPGIYTLWVECHRQVNQFKGALHKSYPTFQEAEYAFRQYVAGTRGEA 100
           M  TK+YVV+VGR PGIYT W EC +QVNQF+GA  KS+ T  EAE A+    + + G+A
Sbjct: 1   MANTKYYVVWVGRKPGIYTSWPECQKQVNQFQGAKFKSFETKAEAEKAYTAGWSASWGQA 60

Query: 101 N 102
           +
Sbjct: 61  S 61

[Close]

BLAST of Lag0026713 vs. NCBI nr
Match: WP_150456826.1 (ribonuclease H family protein [Paenibacillus spiritus] >KAA9007536.1 ribonuclease H [Paenibacillus spiritus])

HSP 1 Score: 70.5 bits (171), Expect = 1.4e-08
Identity = 33/61 (54.10%), Postives = 41/61 (67.21%), Query Frame = 0


Query: 41  MRATKFYVVFVGRNPGIYTLWVECHRQVNQFKGALHKSYPTFQEAEYAFRQYVAGTRGEA 100
           M  TK+YVV+VGR PGIY  W EC RQ +QF GA +KSY + QEAE A+R    G  G+ 
Sbjct: 1   MAKTKYYVVWVGREPGIYGSWAECRRQTDQFTGAKYKSYESRQEAEEAYRGGWKGRWGQG 60

Query: 101 N 102
           +
Sbjct: 61  S 61

[Close]

BLAST of Lag0026713 vs. NCBI nr
Match: WP_138789662.1 (ribonuclease H family protein [Paenibacillus thermoaerophilus] >TMV13896.1 ribonuclease H [Paenibacillus thermoaerophilus])

HSP 1 Score: 70.1 bits (170), Expect = 1.8e-08
Identity = 32/59 (54.24%), Postives = 41/59 (69.49%), Query Frame = 0


Query: 41  MRATKFYVVFVGRNPGIYTLWVECHRQVNQFKGALHKSYPTFQEAEYAFRQYVAGTRGE 100
           M  TK+YVV+VGR PGIYT W EC  QV++F+GA  KSY +  EAE AF+   +G  G+
Sbjct: 1   MAKTKYYVVWVGRKPGIYTTWAECQEQVHRFEGARFKSYESKAEAEAAFQSGWSGEGGQ 59

[Close]

BLAST of Lag0026713 vs. NCBI nr
Match: GAK01363.1 (LOW QUALITY PROTEIN: ribonuclease HI-related protein 3 [Geomicrobium sp. JCM 19055])

HSP 1 Score: 68.2 bits (165), Expect = 6.9e-08
Identity = 29/50 (58.00%), Postives = 36/50 (72.00%), Query Frame = 0


Query: 41 MRATKFYVVFVGRNPGIYTLWVECHRQVNQFKGALHKSYPTFQEAEYAFR 91
          M   K+YVV+ GR PGIYT W EC  +V  FKGA  KSYPT++EA+ AF+
Sbjct: 1  MAKKKYYVVWKGRKPGIYTTWAECEAEVKGFKGARFKSYPTYEEAKQAFQ 50

[Close]

BLAST of Lag0026713 vs. ExPASy Swiss-Prot
Match: Q9KEI9 (Ribonuclease H OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=rnhA PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.9e-09
Identity = 35/83 (42.17%), Postives = 44/83 (53.01%), Query Frame = 0


Query: 41  MRATKFYVVFVGRNPGIYTLWVECHRQVNQFKGALHKSYPTFQEAEYAFRQYVAGTRGEA 100
           M  +K+YVV+ GR PGIYT W  C  QV  + GA  KSYP+ +EAE AFR    G     
Sbjct: 1   MAKSKYYVVWNGRKPGIYTSWSACEAQVKGYTGAKFKSYPSKEEAEAAFR----GEEATP 60

Query: 101 NLVDQHDVCRNL--PVGGYGTVG 122
            L  +  +  +L   VG  G  G
Sbjct: 61  KLAKEEIIWESLSVDVGSQGNPG 79

[Close]

BLAST of Lag0026713 vs. ExPASy Swiss-Prot
Match: Q07762 (Ribonuclease H OS=Crithidia fasciculata OX=5656 GN=RNH1 PE=3 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 1.5e-05
Identity = 22/40 (55.00%), Postives = 27/40 (67.50%), Query Frame = 0


Query: 46  FYVVFVGRNPGIYTLWVECHRQVNQFKGALHKSYPTFQEA 86
           FYVV VGR  GIY+ W +C  QV  F GA++KS+ T  EA
Sbjct: 156 FYVVAVGRQRGIYSTWDQCSEQVKGFSGAVYKSFRTLSEA 195

[Close]

BLAST of Lag0026713 vs. ExPASy Swiss-Prot
Match: Q04740 (Ribonuclease H OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RNH1 PE=1 SV=2)

HSP 1 Score: 45.8 bits (107), Expect = 4.8e-04
Identity = 18/45 (40.00%), Postives = 28/45 (62.22%), Query Frame = 0


Query: 42 RATKFYVVFVGRNPGIYTLWVECHRQVNQFKGALHKSYPTFQEAE 87
          R   FY V  GR  GIY  W EC  QV+ + GA++K + ++++A+
Sbjct: 3  RQGNFYAVRKGRETGIYNTWNECKNQVDGYGGAIYKKFNSYEQAK 47

The following BLAST results are available for this feature:

BLAST of Lag0026713 vs. NCBI nr


Match Name	E-value	Identity	Description
RYR74402.1	1.1e-10	55.00	hypothetical protein Ahy_A02g009104 [Arachis hypogaea]	[more]
WP_138879431.1	1.4e-08	54.10	ribonuclease H family protein [Paenibacillus mesophilus] >TMV50118.1 ribonucleas...	[more]
WP_150456826.1	1.4e-08	54.10	ribonuclease H family protein [Paenibacillus spiritus] >KAA9007536.1 ribonucleas...	[more]
WP_138789662.1	1.8e-08	54.24	ribonuclease H family protein [Paenibacillus thermoaerophilus] >TMV13896.1 ribon...	[more]
GAK01363.1	6.9e-08	58.00	LOW QUALITY PROTEIN: ribonuclease HI-related protein 3 [Geomicrobium sp. JCM 190...	[more]

BLAST of Lag0026713 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9KEI9	2.9e-09	42.17	Ribonuclease H OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 73...	[more]
Q07762	1.5e-05	55.00	Ribonuclease H OS=Crithidia fasciculata OX=5656 GN=RNH1 PE=3 SV=1	[more]
Q04740	4.8e-04	40.00	Ribonuclease H OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=55929...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011320	Ribonuclease H1, N-terminal	PFAM	PF01693	Cauli_VI	coord: 45..86 e-value: 4.6E-17 score: 62.0
IPR037056	Ribonuclease H1, N-terminal domain superfamily	GENE3D	3.40.970.10		coord: 43..94 e-value: 3.3E-17 score: 64.4
IPR009027	Ribosomal protein L9/RNase H1, N-terminal	SUPERFAMILY	55658	L9 N-domain-like	coord: 45..87

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0026713.1	Lag0026713.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity