Lag0026685 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0026685
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNA-directed DNA polymerase (Reverse transcriptase)-related family protein
Locationchr10: 40552040 .. 40552468 (-)
RNA-Seq ExpressionLag0026685
SyntenyLag0026685
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCACTGGAAAAGATGGGAAGAGCTCTACAAACCAAAAGAGGATAGAGGTTTAAACTTTCGTGAATTGGTTTCATTCAACCAGGCGATGATTGCTAAACAGGTCTGGAGGGTTTTGTGCAACCCACAGCTAAAAATTTCTAGAGTTCTTCGTGGGAGATATTTTCCGAACTCTACGGTTTTAAATTCGTCTGCTCGTTCTTCCTCATCTTTCTTTTGGAAGGGTTTTGTATGGGGAATGAAACTTCTGAAAAAAAGTATAAGGAAAAATATGGGGAATGGGAAGGCAATATACATGTTCAGGGACCCGTGGCTTCCAAGACCTACTACTTTTAAGGTGATTTCATATCCAAGGCCTGATATGGAGAATGCGGTTGTGGCAGAGTTTGTCACGCCTTCTTTACAATGGGATATAGGGAAACTCTATTAA

mRNA sequence

ATGCACTGGAAAAGATGGGAAGAGCTCTACAAACCAAAAGAGGATAGAGGTTTAAACTTTCGTGAATTGGTTTCATTCAACCAGGCGATGATTGCTAAACAGGTCTGGAGGGTTTTGTGCAACCCACAGCTAAAAATTTCTAGAGTTCTTCGTGGGAGATATTTTCCGAACTCTACGGTTTTAAATTCGTCTGCTCGTTCTTCCTCATCTTTCTTTTGGAAGGGTTTTGTATGGGGAATGAAACTTCTGAAAAAAAGTATAAGGAAAAATATGGGGAATGGGAAGGCAATATACATGTTCAGGGACCCGTGGCTTCCAAGACCTACTACTTTTAAGGTGATTTCATATCCAAGGCCTGATATGGAGAATGCGGTTGTGGCAGAGTTTGTCACGCCTTCTTTACAATGGGATATAGGGAAACTCTATTAA

Coding sequence (CDS)

ATGCACTGGAAAAGATGGGAAGAGCTCTACAAACCAAAAGAGGATAGAGGTTTAAACTTTCGTGAATTGGTTTCATTCAACCAGGCGATGATTGCTAAACAGGTCTGGAGGGTTTTGTGCAACCCACAGCTAAAAATTTCTAGAGTTCTTCGTGGGAGATATTTTCCGAACTCTACGGTTTTAAATTCGTCTGCTCGTTCTTCCTCATCTTTCTTTTGGAAGGGTTTTGTATGGGGAATGAAACTTCTGAAAAAAAGTATAAGGAAAAATATGGGGAATGGGAAGGCAATATACATGTTCAGGGACCCGTGGCTTCCAAGACCTACTACTTTTAAGGTGATTTCATATCCAAGGCCTGATATGGAGAATGCGGTTGTGGCAGAGTTTGTCACGCCTTCTTTACAATGGGATATAGGGAAACTCTATTAA

Protein sequence

MHWKRWEELYKPKEDRGLNFRELVSFNQAMIAKQVWRVLCNPQLKISRVLRGRYFPNSTVLNSSARSSSSFFWKGFVWGMKLLKKSIRKNMGNGKAIYMFRDPWLPRPTTFKVISYPRPDMENAVVAEFVTPSLQWDIGKLY
Homology
BLAST of Lag0026685 vs. NCBI nr
Match: XP_022131662.1 (uncharacterized protein LOC111004787 [Momordica charantia])

HSP 1 Score: 153.7 bits (387), Expect = 1.2e-33
Identity = 68/138 (49.28%), Postives = 95/138 (68.84%), Query Frame = 0

Query: 1   MHWKRWEELYKPKEDRGLNFRELVSFNQAMIAKQVWRVLCNPQLKISRVLRGRYFPNSTV 60
           +HW  WE +  PKE  GLNFR+L  FNQA++AKQVWRVL NP + +SRVL+ +YF +S V
Sbjct: 61  LHWMSWERMCLPKELGGLNFRDLEGFNQALVAKQVWRVLQNPNILVSRVLKAKYFHDSLV 120

Query: 61  LNSSARSSSSFFWKGFVWGMKLLKKSIRKNMGNGKAIYMFRDPWLPRPTTFKVISYPRPD 120
           L ++   + S+FWKGF+WG  LL K +R  +GNG  I +F DPW+PRP +F+ I+ P   
Sbjct: 121 LQATHLRNQSYFWKGFIWGRDLLIKGLRSRVGNGSTINIFSDPWIPRPYSFRPITAPFGP 180

Query: 121 MENAVVAEFVTPSLQWDI 139
            +   VA+ + P+ QWD+
Sbjct: 181 YD-VKVADLINPNGQWDV 197

BLAST of Lag0026685 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 151.4 bits (381), Expect = 6.0e-33
Identity = 67/138 (48.55%), Postives = 93/138 (67.39%), Query Frame = 0

Query: 1    MHWKRWEELYKPKEDRGLNFRELVSFNQAMIAKQVWRVLCNPQLKISRVLRGRYFPNSTV 60
            +HW +W  +  PKE  GLNFR+L  FNQA++AK VWR L +P L +S+VL+ +YF ++++
Sbjct: 929  LHWMKWGRMCYPKECGGLNFRDLEGFNQALVAKHVWRFLQHPNLLVSKVLKHKYFKDTSL 988

Query: 61   LNSSARSSSSFFWKGFVWGMKLLKKSIRKNMGNGKAIYMFRDPWLPRPTTFKVISYPRPD 120
            L +S  S SS+FWKGF+WG  LL K +R  +GNG  I  F DPWLPRPTTFK + +    
Sbjct: 989  LQASNNSKSSYFWKGFLWGRDLLVKGLRLRVGNGSTIKAFSDPWLPRPTTFKPLRFNNGA 1048

Query: 121  MENAVVAEFVTPSLQWDI 139
            ++   VA F+T    WD+
Sbjct: 1049 LD-TTVASFITADGNWDV 1065

BLAST of Lag0026685 vs. NCBI nr
Match: XP_024950112.1 (uncharacterized protein LOC112496847 [Citrus sinensis])

HSP 1 Score: 136.7 bits (343), Expect = 1.5e-28
Identity = 64/141 (45.39%), Postives = 94/141 (66.67%), Query Frame = 0

Query: 1    MHWKRWEELYKPKEDRGLNFRELVSFNQAMIAKQVWRVLCNPQLKISRVLRGRYFPNSTV 60
            +HW++WE+L + K   GL FRE   FNQA++AKQ WR+L  P   +SRVL+ RYF NS+ 
Sbjct: 871  IHWRKWEKLSQAKIRGGLGFREFSCFNQALVAKQAWRLLQYPNSLVSRVLQARYFRNSSF 930

Query: 61   LNSSARSSSSFFWKGFVWGMKLLKKSIRKNMGNGKAIYMFRDPWLPRPTTFKVISYPRPD 120
            L + A +++S+ W+  +WG +++KK +R  +GNGK I +F D WLPRP TF+ I +P   
Sbjct: 931  LCAKAGANASYIWRSIMWGRQVIKKGMRWRIGNGKKIAIFSDNWLPRPETFRPI-FPLSL 990

Query: 121  MENAVVAEFVTPSLQWDIGKL 142
              ++VVA+ +    QWD  KL
Sbjct: 991  PVSSVVADLIKADNQWDEIKL 1010

BLAST of Lag0026685 vs. NCBI nr
Match: XP_006491472.1 (uncharacterized protein LOC102626455 [Citrus sinensis])

HSP 1 Score: 135.2 bits (339), Expect = 4.4e-28
Identity = 57/141 (40.43%), Postives = 92/141 (65.25%), Query Frame = 0

Query: 1    MHWKRWEELYKPKEDRGLNFRELVSFNQAMIAKQVWRVLCNPQLKISRVLRGRYFPNSTV 60
            +HW RW+ + K K   GL FR+L SFNQA++AKQ WR++  P   ++RV++ RY+ NST 
Sbjct: 930  IHWARWDSMSKAKRRGGLGFRDLPSFNQALVAKQGWRLVRYPNSLMARVMKARYYKNSTF 989

Query: 61   LNSSARSSSSFFWKGFVWGMKLLKKSIRKNMGNGKAIYMFRDPWLPRPTTFKVISYPRPD 120
             N+   S+ SF W+  +WG +++KK +R  +G+GK + +++D W+PRP TF+ IS P+  
Sbjct: 990  WNAKVGSNPSFIWRSILWGSQVIKKGVRWRIGDGKKVLVYKDKWIPRPATFQPIS-PKTL 1049

Query: 121  MENAVVAEFVTPSLQWDIGKL 142
                VVA+ +    +W + +L
Sbjct: 1050 PHETVVADLIDSENKWRVDRL 1069

BLAST of Lag0026685 vs. NCBI nr
Match: XP_030509050.1 (uncharacterized protein LOC115723712 [Cannabis sativa])

HSP 1 Score: 132.5 bits (332), Expect = 2.9e-27
Identity = 61/141 (43.26%), Postives = 86/141 (60.99%), Query Frame = 0

Query: 1   MHWKRWEELYKPKEDRGLNFRELVSFNQAMIAKQVWRVLCNPQLKISRVLRGRYFPNSTV 60
           +HWK W+ L K K   G+ FR  V FNQA++AKQ WR+L +P   I+RVL+ RYF     
Sbjct: 849 IHWKNWKFLCKSKVHGGMGFRNFVHFNQALLAKQAWRILEDPSSLIARVLKSRYFATGVF 908

Query: 61  LNSSARSSSSFFWKGFVWGMKLLKKSIRKNMGNGKAIYMFRDPWLPRPTTFKVISYPRPD 120
           L ++A +  S  WK  VWG +LL K +R  +G+G  +     PW+P P+ FK + +  PD
Sbjct: 909 LEATAGAQPSLTWKSIVWGKELLLKGLRWRIGSGADVNCVSQPWIPGPSNFKPLLFLSPD 968

Query: 121 MENAVVAEFVTPSLQWDIGKL 142
             +  VA+F+T S QWD+ KL
Sbjct: 969 -RDVKVADFITHSRQWDVPKL 988

BLAST of Lag0026685 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.4e-16
Identity = 40/104 (38.46%), Postives = 67/104 (64.42%), Query Frame = 0

Query: 3   WKRWEELYKPKE-DRGLNFRELVSFNQAMIAKQVWRVLCNPQLKISRVLRGRYFPNSTVL 62
           W  W++L K KE D GL FR+L  FNQA++AKQ +R++  P   +SR+LR RYFP+S+++
Sbjct: 41  WVAWQKLCKSKEDDGGLGFRDLGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMM 100

Query: 63  NSSARSSSSFFWKGFVWGMKLLKKSIRKNMGNGKAIYMFRDPWL 106
             S  +  S+ W+  + G +LL + + + +G+G    ++ D W+
Sbjct: 101 ECSVGTRPSYAWRSIIHGRELLSRGLLRTIGDGIHTKVWLDRWI 144

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131662.11.2e-3349.28uncharacterized protein LOC111004787 [Momordica charantia][more]
XP_022158377.16.0e-3348.55uncharacterized protein LOC111024874 [Momordica charantia][more]
XP_024950112.11.5e-2845.39uncharacterized protein LOC112496847 [Citrus sinensis][more]
XP_006491472.14.4e-2840.43uncharacterized protein LOC102626455 [Citrus sinensis][more]
XP_030509050.12.9e-2743.26uncharacterized protein LOC115723712 [Cannabis sativa][more]
Match NameE-valueIdentityDescription
P932951.4e-1638.46Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 1..120
NoneNo IPR availablePANTHERPTHR33116:SF41SUBFAMILY NOT NAMEDcoord: 1..120

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0026685.1Lag0026685.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016740 transferase activity