Lag0026659 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0026659
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	ribonuclease MC-like
Location	chr10: 40244923 .. 40245735 (+)
RNA-Seq Expression	Lag0026659
Synteny	Lag0026659

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGACATGGGGAAGAGAGAAATTGTGTTGGTTTTGAGCATGTTGTTGCTATTGATATCTACCTCGGAAGGTCAAGTGAACCAATTCGACTACTTCCAACTTATTATTCAATGGCAACCAGCCGCTTGTAGTAGCTCAACTCTACCATCTCCATGTAGGAATACTCCACAAAACAGATTCAGTATCCATGGTGTTTGGCCCAGCCTCAACTCTGGGGCTTCTGCACAATGTTCTGGCACTCCGCCCTATGATGGTAGACTGGTAATTAATCATATTCATTTTCATAAACCCAACTTCATTCAAAAATTAATGGAAGATCGTTTAATCGAATGGTTCGTTTGATTCTCGTAGATGACAAGGGCAATCCGAGATAGGCTGGATCAAGATTGGCCAAGCGTGGTTTATGGGATAAATCCAACACTGTGGAGCCACGAGTGGACAGCCCATGGAACTTGTTCTAACTCATCTATGAACCAAGTGCAGTATTTTACGTTTGGACTGGATGAATTTGCTAAGTACAATTTGCGATCCACGCTGGCTGCTTACGGTATCAATCCAGACGGGAAGGAATACTCGAGGGGCGATGTTGAAGCTGCAATTTTCTGTGGAACAGGTGGAAAGTGGGCAGCCCTTCGCTGCAACAGAAACAGGGCTGGTCAGAAACAATTGTATGAAATAGCTTTGTGCTTCACAAAGGATAATCGACAGCTTATGGACTGCCCCTTAAATGCCGTTACATGTGGCAGTAAATTCTTGTGGAGTTCATGGAGTAATCGCGTGGAAGACAAACCTGAAATTGCATCTAGTTGA

mRNA sequence

ATGGAAGACATGGGGAAGAGAGAAATTGTGTTGGTTTTGAGCATGTTGTTGCTATTGATATCTACCTCGGAAGGTCAAGTGAACCAATTCGACTACTTCCAACTTATTATTCAATGGCAACCAGCCGCTTGTAGTAGCTCAACTCTACCATCTCCATGTAGGAATACTCCACAAAACAGATTCAGTATCCATGGTGTTTGGCCCAGCCTCAACTCTGGGGCTTCTGCACAATGTTCTGGCACTCCGCCCTATGATGGTAGACTGATGACAAGGGCAATCCGAGATAGGCTGGATCAAGATTGGCCAAGCGTGGTTTATGGGATAAATCCAACACTGTGGAGCCACGAGTGGACAGCCCATGGAACTTGTTCTAACTCATCTATGAACCAAGTGCAGTATTTTACGTTTGGACTGGATGAATTTGCTAAGTACAATTTGCGATCCACGCTGGCTGCTTACGGTATCAATCCAGACGGGAAGGAATACTCGAGGGGCGATGTTGAAGCTGCAATTTTCTGTGGAACAGGTGGAAAGTGGGCAGCCCTTCGCTGCAACAGAAACAGGGCTGGTCAGAAACAATTGTATGAAATAGCTTTGTGCTTCACAAAGGATAATCGACAGCTTATGGACTGCCCCTTAAATGCCGTTACATGTGGCAGTAAATTCTTGTGGAGTTCATGGAGTAATCGCGTGGAAGACAAACCTGAAATTGCATCTAGTTGA

Coding sequence (CDS)

ATGGAAGACATGGGGAAGAGAGAAATTGTGTTGGTTTTGAGCATGTTGTTGCTATTGATATCTACCTCGGAAGGTCAAGTGAACCAATTCGACTACTTCCAACTTATTATTCAATGGCAACCAGCCGCTTGTAGTAGCTCAACTCTACCATCTCCATGTAGGAATACTCCACAAAACAGATTCAGTATCCATGGTGTTTGGCCCAGCCTCAACTCTGGGGCTTCTGCACAATGTTCTGGCACTCCGCCCTATGATGGTAGACTGATGACAAGGGCAATCCGAGATAGGCTGGATCAAGATTGGCCAAGCGTGGTTTATGGGATAAATCCAACACTGTGGAGCCACGAGTGGACAGCCCATGGAACTTGTTCTAACTCATCTATGAACCAAGTGCAGTATTTTACGTTTGGACTGGATGAATTTGCTAAGTACAATTTGCGATCCACGCTGGCTGCTTACGGTATCAATCCAGACGGGAAGGAATACTCGAGGGGCGATGTTGAAGCTGCAATTTTCTGTGGAACAGGTGGAAAGTGGGCAGCCCTTCGCTGCAACAGAAACAGGGCTGGTCAGAAACAATTGTATGAAATAGCTTTGTGCTTCACAAAGGATAATCGACAGCTTATGGACTGCCCCTTAAATGCCGTTACATGTGGCAGTAAATTCTTGTGGAGTTCATGGAGTAATCGCGTGGAAGACAAACCTGAAATTGCATCTAGTTGA

Protein sequence

MEDMGKREIVLVLSMLLLLISTSEGQVNQFDYFQLIIQWQPAACSSSTLPSPCRNTPQNRFSIHGVWPSLNSGASAQCSGTPPYDGRLMTRAIRDRLDQDWPSVVYGINPTLWSHEWTAHGTCSNSSMNQVQYFTFGLDEFAKYNLRSTLAAYGINPDGKEYSRGDVEAAIFCGTGGKWAALRCNRNRAGQKQLYEIALCFTKDNRQLMDCPLNAVTCGSKFLWSSWSNRVEDKPEIASS

Homology

[Close]

BLAST of Lag0026659 vs. NCBI nr
Match: XP_038903348.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 318.9 bits (816), Expect = 3.7e-83
Identity = 151/224 (67.41%), Postives = 179/224 (79.91%), Query Frame = 0


Query: 4   MGKREIVLVLSMLLLLISTSEGQVNQFDYFQLIIQWQPAACSSSTLPSPCRNTPQNRFSI 63
           MGKREI+LVLS+LLLL+ +S+G +N+FDY+Q+I QWQPAACSS+TL SPC   P+NRF+I
Sbjct: 1   MGKREIMLVLSILLLLVGSSKG-LNEFDYYQVIFQWQPAACSSATLKSPCVKRPENRFTI 60

Query: 64  HGVWPSLNSGASAQCSGTPPYDGRLMTRAIRDRLDQDWPSVVYGINPTLWSHEWTAHGTC 123
           HGVWPSL SG + +CSGTPPYDG  +  AIR RLDQDWPSVVYG NPTLWSHEW  HGTC
Sbjct: 61  HGVWPSLTSGNATKCSGTPPYDGNKI-YAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTC 120

Query: 124 SNSSMNQVQYFTFGLDEFAKYNLRSTLAAYGINPDGKEYSRGDVEAAIFCGTGGKWAALR 183
           SN  ++Q  YF FGL  F KYN+ S L  YGINPDG +YS   +E+AI+  T  KW ALR
Sbjct: 121 SNYYLDQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNAT-VKWPALR 180

Query: 184 CNRNR-AGQKQLYEIALCFTKDNRQLMDCPLNAVTCGSKFLWSS 227
           CN+N+ +G+KQLYEIALCFTK NR L DCP NAVTCG+KF W+S
Sbjct: 181 CNKNKSSGKKQLYEIALCFTKVNRALQDCPYNAVTCGNKFSWNS 221

[Close]

BLAST of Lag0026659 vs. NCBI nr
Match: XP_022158939.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 278.1 bits (710), Expect = 7.2e-71
Identity = 131/226 (57.96%), Postives = 170/226 (75.22%), Query Frame = 0


Query: 4   MGKREIVLVLSMLLLLISTSEGQVNQFDYFQLIIQWQPAACSSSTLPSPCRNTPQNRFSI 63
           MGKREI+LVLSM  L IS+SEG VN+FDY+Q+++QWQPA CSS+TL + C   P++R+S+
Sbjct: 1   MGKREILLVLSM-FLAISSSEG-VNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSV 60

Query: 64  HGVWPSLNSGASAQCSGTPPYDGRLMTRAIRDRLDQDWPSVVYGINPTLWSHEWTAHGTC 123
           HGVWPSL+SG    CSGTPPYD R M   I+ RLDQDWP+V+ G NP  WS+EWT HGTC
Sbjct: 61  HGVWPSLSSGYLPTCSGTPPYDAR-MILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTC 120

Query: 124 SNSSMNQVQYFTFGLDEFAKYNLRSTLAAYGINPDGKEYSRGDVEAAIFCGTGGKWAALR 183
           S++ +NQ  YF FGL  +  Y+L+S L  YGI P+G++Y++  V+ AI+  T GKW ALR
Sbjct: 121 SSAYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSAT-GKWPALR 180

Query: 184 CNRN-RAGQKQLYEIALCFTKDNRQLMDCPLNAVTCGSKFLWSSWS 229
           CN N R G  QL+EI+LCF+K+ + L+DCP N VTC   F+W+SWS
Sbjct: 181 CNNNYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWS 222

[Close]

BLAST of Lag0026659 vs. NCBI nr
Match: XP_022156540.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 262.3 bits (669), Expect = 4.1e-66
Identity = 131/228 (57.46%), Postives = 159/228 (69.74%), Query Frame = 0


Query: 4   MGKR-EIVLVLSMLLLLISTSEGQVNQFDYFQLIIQWQPAACSSSTLPSPCRNTPQNRFS 63
           M KR EIVL+LSM LLLI  SEG VN+FDY+Q+++QWQPA CSSSTL   C   P  RFS
Sbjct: 1   MAKRSEIVLILSM-LLLIGWSEG-VNEFDYYQVVLQWQPATCSSSTLVDECVQPPDERFS 60

Query: 64  IHGVWPSLNSGASAQCSGTPPYDGRLMTRAIRDRLDQDWPSVVYGINPTLWSHEWTAHGT 123
           IHGVWPSL SG  A CSGTPPYDG  +  +IR RL QDWPSVV+ ++P  WSHEWT HGT
Sbjct: 61  IHGVWPSLLSG-DAVCSGTPPYDGTQLNSSIRARLHQDWPSVVHRVDPPFWSHEWTLHGT 120

Query: 124 CSNSSMNQVQYFTFGLDEFAKYNLRSTLAAYGINPDGKEYSRGDVEAAIFCGTGGKWAAL 183
           CS + ++Q  YF FGL  +  Y++   L  Y + P+G  Y+R DVE  I+  T GKW AL
Sbjct: 121 CSYAYLDQFHYFDFGLFVYGNYDVLGILNYYNVYPNGNLYARSDVENIIYSAT-GKWPAL 180

Query: 184 RCNRN-RAGQKQLYEIALCFTKDNRQLMDCPLNAVTCGSKFLWSSWSN 230
           RCN N  +G  QL+EI+LCF K +  L DCP NAV C   F+W+SWS+
Sbjct: 181 RCNNNFWSGDVQLHEISLCFRKQDFSLEDCPYNAVKCNDYFVWNSWSS 224

[Close]

BLAST of Lag0026659 vs. NCBI nr
Match: XP_022159354.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 244.2 bits (622), Expect = 1.1e-60
Identity = 118/225 (52.44%), Postives = 153/225 (68.00%), Query Frame = 0


Query: 4   MGKREIVLVLSMLLLLISTSEGQVNQFDYFQLIIQWQPAACSSSTLPSPCRNTPQNRFSI 63
           M K+ IVLV S L+++ S        ++Y+Q+ +QWQPA CS + L +PC NTPQ+RFSI
Sbjct: 1   MEKKGIVLVFSTLVVIASC------DYNYYQVTLQWQPATCSDANLQTPCVNTPQDRFSI 60

Query: 64  HGVWPSLNSGASAQCSGTPPYDGRLMTRAIRDRLDQDWPSVVYGINPTLWSHEWTAHGTC 123
           HGVWP+L+SG+  QC GTP YDG  ++  IR+RL+ DWPSV+YG NPTLWSHEWT HGTC
Sbjct: 61  HGVWPTLSSGSYPQCPGTPAYDGSKIS-TIRNRLNADWPSVIYGTNPTLWSHEWTTHGTC 120

Query: 124 SNSSMNQVQYFTFGLDEFAKYNLRSTLAAYGINPDGKEYSRGDVEAAIFCGTGGKWAALR 183
           S  S++Q+ YFTFGLD +AKY++ S L    I P    YS+  +  AI   T  K   LR
Sbjct: 121 SYPSLSQLDYFTFGLDTYAKYDIYSALDKEDIYPSEDVYSKSSINNAIRKIT-QKLPGLR 180

Query: 184 CNRN-RAGQKQLYEIALCFTKDNRQLMDCPLNAVTCGSKFLWSSW 228
           CN N  + ++QL+EI LCF K NR ++DCP N VTCG+   W  W
Sbjct: 181 CNTNDYSRERQLHEIILCFAKSNRAIIDCPSNVVTCGNYVYWYPW 217

[Close]

BLAST of Lag0026659 vs. NCBI nr
Match: AEM42996.1 (S-RNase [Siraitia grosvenorii])

HSP 1 Score: 219.2 bits (557), Expect = 4.0e-53
Identity = 111/230 (48.26%), Postives = 156/230 (67.83%), Query Frame = 0


Query: 4   MGKREIVLVLSMLLLLISTSEGQVNQFDYFQLIIQWQPAACSSSTLPSP-CRNTPQNRFS 63
           M KREIVLVLSM L+LI++ EG +N+FDY Q+++QWQPA CS+S  P P C   P NR+S
Sbjct: 1   MAKREIVLVLSM-LVLIASCEG-LNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYS 60

Query: 64  IHGVWPSLNSGASAQCSGTPPYDGRLMTRAIRDRLDQDWPSVVYGINPTLWSHEWTAHGT 123
           IHGVWPSL SGA+ QCSG+ P++   ++  ++  + + WP+++ G N  LW HEW  HGT
Sbjct: 61  IHGVWPSLYSGAATQCSGS-PFNANAIS-ILQPGISEIWPNIINGNNLWLWGHEWDKHGT 120

Query: 124 CSNSSM-NQVQYFTFGLDEFAKYNLRSTLAAYGINPDGKEYSRGDVEAAIFCGTGGKWAA 183
           C+   + +Q +YF+F +D +  YNL   L    I P+G+ YSR  V AAI   T GK  A
Sbjct: 121 CTEEILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQIYSRDQVYAAIRAKT-GKTPA 180

Query: 184 LRCNRNR-AGQKQLYEIALCFTKDNRQLMDCPLNAVTCGSKFLWSSWSNR 231
           +RCN NR  G++Q++E++LC+  D   ++DCPLNA  C + F+W SWS +
Sbjct: 181 VRCNYNRWTGEQQMHEVSLCYNHDASAVVDCPLNANKCDNYFVWYSWSTQ 225

[Close]

BLAST of Lag0026659 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.9e-27
Identity = 64/196 (32.65%), Postives = 99/196 (50.51%), Query Frame = 0


Query: 30  FDYFQLIIQWQPAACSSSTLPSPCRNTPQNRFSIHGVWPSLNSGASAQCSGTPPYDGRLM 89
           FD F  + QW PA CS     S C  +    F+IHG+WP  +  +   C    P+D   +
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGS-CPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKI 60

Query: 90  TRAIRDRLDQDWPSVVYGINPTLWSHEWTAHGTCSNSSMNQVQYFTFGLDEFAKYNLRST 149
           +  ++ +L+  WP+V+   N   WSHEWT HGTCS S+ NQ  YF   +D    Y++   
Sbjct: 61  SH-LQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGA 120

Query: 150 LAAYGINPDGKEYSRGDVEAAIFCGTGGKWAALRCNRN-RAGQKQLYEIALCFTKDNRQL 209
           L  +   P+G+  SR  ++        GK+  LRC  + +     L ++  CF +D   L
Sbjct: 121 LRPHAAGPNGRTKSRQAIK-GFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTL 180

Query: 210 MDCPLNAVTCGSKFLW 225
           +DC  +  TCG+ F++
Sbjct: 181 IDCTRD--TCGANFIF 191

[Close]

BLAST of Lag0026659 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 6.6e-27
Identity = 66/209 (31.58%), Postives = 105/209 (50.24%), Query Frame = 0


Query: 12  VLSMLLLL--ISTSEGQVNQFDYFQLIIQWQPAAC-SSSTLPSPCRNTPQNRFSIHGVWP 71
           ++S+L++L  + ++      FD+F  + QW  + C +      P    P   F IHG+WP
Sbjct: 10  LISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWP 69

Query: 72  SLNSGA-SAQCSGTPPYDGRLMTRAIRDRLDQDWPSVV--YGINPTLWSHEWTAHGTCSN 131
           +   G   + C  + P+D   ++  +   + + WP++    G     W HEW  HGTCS 
Sbjct: 70  NYKDGTYPSNCDASKPFDSSTISDLLTS-MKKSWPTLACPSGSGEAFWEHEWEKHGTCSE 129

Query: 132 SSMNQVQYFTFGLDEFAKYNLRSTLAAYGINPDGKEYSRGDVEAAIFCGTG-GKWAALRC 191
           S ++Q +YF   L+   K NL   L   GINPDGK YS   +  +I    G   W  + C
Sbjct: 130 SVIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPW--VEC 189

Query: 192 NRNRAGQKQLYEIALCFTKDNRQLMDCPL 214
           NR+ +G  QLY++ LC  +    L++CP+
Sbjct: 190 NRDGSGNSQLYQVYLCVDRSGSGLIECPV 215

[Close]

BLAST of Lag0026659 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 5.2e-24
Identity = 63/188 (33.51%), Postives = 94/188 (50.00%), Query Frame = 0


Query: 30  FDYFQLIIQWQPAACSS-STLPSPCRNTPQNRFSIHGVWPSLNSGASAQ-CSGTPPYDGR 89
           FD+F  ++QW  A C S  +   P    P   F IHG+WP+  +G   Q C+    +D  
Sbjct: 22  FDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRFDDL 81

Query: 90  LMTRAIRDRLDQDWPSVVYGINPTL--WSHEWTAHGTCSNSSMNQVQYFTFGLDEFAKYN 149
            ++  + D L ++WP++    N  +  W+HEW  HGTC+ S ++Q  YF  GL    K N
Sbjct: 82  RVSDLMSD-LQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQKAN 141

Query: 150 LRSTLAAYGINPDGKEYSRGDVEAAIFCGTGGKWAALRCNRNRAGQKQLYEIALCFTKDN 209
           L   L   GI PD K Y   D+E  I    G     + CN++ +   QLY+I LC     
Sbjct: 142 LLHALTNAGIKPDDKFYEMKDIENTIKQVVGFA-PGIECNKDSSHNSQLYQIYLCVDTSA 201

Query: 210 RQLMDCPL 214
            + ++CP+
Sbjct: 202 SKFINCPV 207

[Close]

BLAST of Lag0026659 vs. ExPASy Swiss-Prot
Match: Q40965 (Ribonuclease S-2 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 6.4e-22
Identity = 65/206 (31.55%), Postives = 109/206 (52.91%), Query Frame = 0


Query: 11  LVLSMLLLLISTSEGQVNQFDYFQLIIQWQPAACSSSTLPSPCRNTPQNRFSIHGVWPSL 70
           +V S+ +L++S+S     ++DYFQ   Q+Q A C+S+  P+PC++ P   F++HG+WPS 
Sbjct: 7   MVFSLNVLILSSSAA---RYDYFQFTQQYQQAFCNSN--PTPCKDPPDKLFTVHGLWPST 66

Query: 71  NSGASAQCSGTPPYDGRLMTRAIRDRLDQDWPSVV-YGINPTLWSHEWTAHGTCSNSSM- 130
             G   +   T  Y  R + R +  +L+  WP+V     N   W  +W  HG+C++ ++ 
Sbjct: 67  KVGRDPEYCKTKRY--RKIQR-LEPQLEIIWPNVSDRKANRGFWRKQWYKHGSCASPALP 126

Query: 131 NQVQYFTFGLDEF--AKYNLRSTLAAYGINPDGKEYSRGDVEAAIFCGTGGKWAALRCNR 190
           NQ  YF   +  F   K N+   L+   I P+GK  +  +++ AI  GT      L+C +
Sbjct: 127 NQKHYFETVIRMFLAEKQNVSRILSMATIEPEGKNRTLLEIQNAIRAGTNNMIPKLKCQK 186

Query: 191 NRAGQKQLYEIALCFTKDNRQLMDCP 213
              G  +L E+ LC   +  Q ++CP
Sbjct: 187 VN-GMTELVEVTLCHDSNLTQFINCP 203

[Close]

BLAST of Lag0026659 vs. ExPASy Swiss-Prot
Match: Q40966 (Ribonuclease S-4 OS=Pyrus pyrifolia OX=3767 PE=1 SV=2)

HSP 1 Score: 104.8 bits (260), Expect = 1.4e-21
Identity = 60/206 (29.13%), Postives = 103/206 (50.00%), Query Frame = 0


Query: 11  LVLSMLLLLISTSEGQVNQFDYFQLIIQWQPAACSSSTLPSPCRNTPQNRFSIHGVWPSL 70
           +VLS+++L+ S S      FDYFQ   Q+QPA C+S+  P+PC +     F++HG+WPS 
Sbjct: 12  MVLSLIVLIFSAS---TVGFDYFQFTQQYQPAVCNSN--PTPCNDPTDKLFTVHGLWPSN 71

Query: 71  NSGASAQCSGTPPYDGRLMTRAIRDRLDQDWPSVVYGINPT-LWSHEWTAHGTCSNSSMN 130
            +G   +   T   + + +   +  +L+  WP+V+   +    W  EW  HGTC   ++ 
Sbjct: 72  RNGPDPEKCKTTTMNSQKIGN-MTAQLEIIWPNVLNRSDHVGFWEREWLKHGTCGYPTIK 131

Query: 131 QVQYFTFGLDEF---AKYNLRSTLAAYGINPDGKEYSRGDVEAAIFCGTGGKWAALRCNR 190
              ++   + +     K N+ + L+   I P+G   S  D+E AI  G        +C +
Sbjct: 132 DDMHYLKTVIKMYITQKQNVSAILSKATIQPNGNNRSLVDIENAIRSGNNNTKPKFKCQK 191

Query: 191 NRAGQKQLYEIALCFTKDNRQLMDCP 213
           N     +L E+ LC  +D  + ++CP
Sbjct: 192 NTRTTTELVEVTLCSNRDLTKFINCP 211

[Close]

BLAST of Lag0026659 vs. ExPASy TrEMBL
Match: A0A6J1E0X1 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 3.5e-71
Identity = 131/226 (57.96%), Postives = 170/226 (75.22%), Query Frame = 0


Query: 4   MGKREIVLVLSMLLLLISTSEGQVNQFDYFQLIIQWQPAACSSSTLPSPCRNTPQNRFSI 63
           MGKREI+LVLSM  L IS+SEG VN+FDY+Q+++QWQPA CSS+TL + C   P++R+S+
Sbjct: 1   MGKREILLVLSM-FLAISSSEG-VNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSV 60

Query: 64  HGVWPSLNSGASAQCSGTPPYDGRLMTRAIRDRLDQDWPSVVYGINPTLWSHEWTAHGTC 123
           HGVWPSL+SG    CSGTPPYD R M   I+ RLDQDWP+V+ G NP  WS+EWT HGTC
Sbjct: 61  HGVWPSLSSGYLPTCSGTPPYDAR-MILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTC 120

Query: 124 SNSSMNQVQYFTFGLDEFAKYNLRSTLAAYGINPDGKEYSRGDVEAAIFCGTGGKWAALR 183
           S++ +NQ  YF FGL  +  Y+L+S L  YGI P+G++Y++  V+ AI+  T GKW ALR
Sbjct: 121 SSAYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSAT-GKWPALR 180

Query: 184 CNRN-RAGQKQLYEIALCFTKDNRQLMDCPLNAVTCGSKFLWSSWS 229
           CN N R G  QL+EI+LCF+K+ + L+DCP N VTC   F+W+SWS
Sbjct: 181 CNNNYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWS 222

[Close]

BLAST of Lag0026659 vs. ExPASy TrEMBL
Match: A0A6J1DQK2 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 2.0e-66
Identity = 131/228 (57.46%), Postives = 159/228 (69.74%), Query Frame = 0


Query: 4   MGKR-EIVLVLSMLLLLISTSEGQVNQFDYFQLIIQWQPAACSSSTLPSPCRNTPQNRFS 63
           M KR EIVL+LSM LLLI  SEG VN+FDY+Q+++QWQPA CSSSTL   C   P  RFS
Sbjct: 1   MAKRSEIVLILSM-LLLIGWSEG-VNEFDYYQVVLQWQPATCSSSTLVDECVQPPDERFS 60

Query: 64  IHGVWPSLNSGASAQCSGTPPYDGRLMTRAIRDRLDQDWPSVVYGINPTLWSHEWTAHGT 123
           IHGVWPSL SG  A CSGTPPYDG  +  +IR RL QDWPSVV+ ++P  WSHEWT HGT
Sbjct: 61  IHGVWPSLLSG-DAVCSGTPPYDGTQLNSSIRARLHQDWPSVVHRVDPPFWSHEWTLHGT 120

Query: 124 CSNSSMNQVQYFTFGLDEFAKYNLRSTLAAYGINPDGKEYSRGDVEAAIFCGTGGKWAAL 183
           CS + ++Q  YF FGL  +  Y++   L  Y + P+G  Y+R DVE  I+  T GKW AL
Sbjct: 121 CSYAYLDQFHYFDFGLFVYGNYDVLGILNYYNVYPNGNLYARSDVENIIYSAT-GKWPAL 180

Query: 184 RCNRN-RAGQKQLYEIALCFTKDNRQLMDCPLNAVTCGSKFLWSSWSN 230
           RCN N  +G  QL+EI+LCF K +  L DCP NAV C   F+W+SWS+
Sbjct: 181 RCNNNFWSGDVQLHEISLCFRKQDFSLEDCPYNAVKCNDYFVWNSWSS 224

[Close]

BLAST of Lag0026659 vs. ExPASy TrEMBL
Match: A0A6J1E266 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 5.6e-61
Identity = 118/225 (52.44%), Postives = 153/225 (68.00%), Query Frame = 0


Query: 4   MGKREIVLVLSMLLLLISTSEGQVNQFDYFQLIIQWQPAACSSSTLPSPCRNTPQNRFSI 63
           M K+ IVLV S L+++ S        ++Y+Q+ +QWQPA CS + L +PC NTPQ+RFSI
Sbjct: 1   MEKKGIVLVFSTLVVIASC------DYNYYQVTLQWQPATCSDANLQTPCVNTPQDRFSI 60

Query: 64  HGVWPSLNSGASAQCSGTPPYDGRLMTRAIRDRLDQDWPSVVYGINPTLWSHEWTAHGTC 123
           HGVWP+L+SG+  QC GTP YDG  ++  IR+RL+ DWPSV+YG NPTLWSHEWT HGTC
Sbjct: 61  HGVWPTLSSGSYPQCPGTPAYDGSKIS-TIRNRLNADWPSVIYGTNPTLWSHEWTTHGTC 120

Query: 124 SNSSMNQVQYFTFGLDEFAKYNLRSTLAAYGINPDGKEYSRGDVEAAIFCGTGGKWAALR 183
           S  S++Q+ YFTFGLD +AKY++ S L    I P    YS+  +  AI   T  K   LR
Sbjct: 121 SYPSLSQLDYFTFGLDTYAKYDIYSALDKEDIYPSEDVYSKSSINNAIRKIT-QKLPGLR 180

Query: 184 CNRN-RAGQKQLYEIALCFTKDNRQLMDCPLNAVTCGSKFLWSSW 228
           CN N  + ++QL+EI LCF K NR ++DCP N VTCG+   W  W
Sbjct: 181 CNTNDYSRERQLHEIILCFAKSNRAIIDCPSNVVTCGNYVYWYPW 217

[Close]

BLAST of Lag0026659 vs. ExPASy TrEMBL
Match: K7NBR4 (S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 1.9e-53
Identity = 111/230 (48.26%), Postives = 156/230 (67.83%), Query Frame = 0


Query: 4   MGKREIVLVLSMLLLLISTSEGQVNQFDYFQLIIQWQPAACSSSTLPSP-CRNTPQNRFS 63
           M KREIVLVLSM L+LI++ EG +N+FDY Q+++QWQPA CS+S  P P C   P NR+S
Sbjct: 1   MAKREIVLVLSM-LVLIASCEG-LNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYS 60

Query: 64  IHGVWPSLNSGASAQCSGTPPYDGRLMTRAIRDRLDQDWPSVVYGINPTLWSHEWTAHGT 123
           IHGVWPSL SGA+ QCSG+ P++   ++  ++  + + WP+++ G N  LW HEW  HGT
Sbjct: 61  IHGVWPSLYSGAATQCSGS-PFNANAIS-ILQPGISEIWPNIINGNNLWLWGHEWDKHGT 120

Query: 124 CSNSSM-NQVQYFTFGLDEFAKYNLRSTLAAYGINPDGKEYSRGDVEAAIFCGTGGKWAA 183
           C+   + +Q +YF+F +D +  YNL   L    I P+G+ YSR  V AAI   T GK  A
Sbjct: 121 CTEEILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQIYSRDQVYAAIRAKT-GKTPA 180

Query: 184 LRCNRNR-AGQKQLYEIALCFTKDNRQLMDCPLNAVTCGSKFLWSSWSNR 231
           +RCN NR  G++Q++E++LC+  D   ++DCPLNA  C + F+W SWS +
Sbjct: 181 VRCNYNRWTGEQQMHEVSLCYNHDASAVVDCPLNANKCDNYFVWYSWSTQ 225

[Close]

BLAST of Lag0026659 vs. ExPASy TrEMBL
Match: A0A6J1D7Q5 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 1.4e-51
Identity = 109/227 (48.02%), Postives = 147/227 (64.76%), Query Frame = 0


Query: 4   MGKREIVLVLSMLLLLISTSEGQVNQFDYFQLIIQWQPAACSSSTLPSPCRNTPQNRFSI 63
           M +REI++++  +L+L++  EG +N+FDY Q++IQWQPA CS    P+ C   P NRFSI
Sbjct: 1   MAQREIIVLVLSMLVLVAICEG-LNEFDYLQVVIQWQPATCSDYNKPA-CFQAPGNRFSI 60

Query: 64  HGVWPSLNSGASAQCSGTPPYDGRLMTRAIRDRLDQDWPSVVYGINPTLWSHEWTAHGTC 123
           HGVWPSL  G   QCSG+ P+D R     ++  + + WP+V  G N  LW HEW  HGTC
Sbjct: 61  HGVWPSLYDGYPTQCSGS-PFD-RNQISGLQPGISEIWPNVATGDNFWLWQHEWDKHGTC 120

Query: 124 SNSSM-NQVQYFTFGLDEFAKYNLRSTLAAYGINPDGKEYSRGDVEAAIFCGTGGKWAAL 183
           S S+  +Q +YF FG+D +   NL+  L+   I P+G+ Y+R  V AAI   T GK  A+
Sbjct: 121 SESTFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKT-GKTPAV 180

Query: 184 RCNRN-RAGQKQLYEIALCFTKDNRQLMDCPLNAVTCGSKFLWSSWS 229
           RCN N R G  QL+EI+LCF KD     DCPLNA  CG+ F+W+SW+
Sbjct: 181 RCNYNSRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWN 222

[Close]

BLAST of Lag0026659 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 122.5 bits (306), Expect = 4.7e-28
Identity = 66/209 (31.58%), Postives = 105/209 (50.24%), Query Frame = 0


Query: 12  VLSMLLLL--ISTSEGQVNQFDYFQLIIQWQPAAC-SSSTLPSPCRNTPQNRFSIHGVWP 71
           ++S+L++L  + ++      FD+F  + QW  + C +      P    P   F IHG+WP
Sbjct: 10  LISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWP 69

Query: 72  SLNSGA-SAQCSGTPPYDGRLMTRAIRDRLDQDWPSVV--YGINPTLWSHEWTAHGTCSN 131
           +   G   + C  + P+D   ++  +   + + WP++    G     W HEW  HGTCS 
Sbjct: 70  NYKDGTYPSNCDASKPFDSSTISDLLTS-MKKSWPTLACPSGSGEAFWEHEWEKHGTCSE 129

Query: 132 SSMNQVQYFTFGLDEFAKYNLRSTLAAYGINPDGKEYSRGDVEAAIFCGTG-GKWAALRC 191
           S ++Q +YF   L+   K NL   L   GINPDGK YS   +  +I    G   W  + C
Sbjct: 130 SVIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPW--VEC 189

Query: 192 NRNRAGQKQLYEIALCFTKDNRQLMDCPL 214
           NR+ +G  QLY++ LC  +    L++CP+
Sbjct: 190 NRDGSGNSQLYQVYLCVDRSGSGLIECPV 215

[Close]

BLAST of Lag0026659 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 112.8 bits (281), Expect = 3.7e-25
Identity = 63/188 (33.51%), Postives = 94/188 (50.00%), Query Frame = 0


Query: 30  FDYFQLIIQWQPAACSS-STLPSPCRNTPQNRFSIHGVWPSLNSGASAQ-CSGTPPYDGR 89
           FD+F  ++QW  A C S  +   P    P   F IHG+WP+  +G   Q C+    +D  
Sbjct: 22  FDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRFDDL 81

Query: 90  LMTRAIRDRLDQDWPSVVYGINPTL--WSHEWTAHGTCSNSSMNQVQYFTFGLDEFAKYN 149
            ++  + D L ++WP++    N  +  W+HEW  HGTC+ S ++Q  YF  GL    K N
Sbjct: 82  RVSDLMSD-LQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQKAN 141

Query: 150 LRSTLAAYGINPDGKEYSRGDVEAAIFCGTGGKWAALRCNRNRAGQKQLYEIALCFTKDN 209
           L   L   GI PD K Y   D+E  I    G     + CN++ +   QLY+I LC     
Sbjct: 142 LLHALTNAGIKPDDKFYEMKDIENTIKQVVGFA-PGIECNKDSSHNSQLYQIYLCVDTSA 201

Query: 210 RQLMDCPL 214
            + ++CP+
Sbjct: 202 SKFINCPV 207

[Close]

BLAST of Lag0026659 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 112.5 bits (280), Expect = 4.9e-25
Identity = 75/231 (32.47%), Postives = 109/231 (47.19%), Query Frame = 0


Query: 4   MGKREIVLVLSMLLLL--ISTSEGQVNQFDYFQLIIQWQPAACSSS-TLPSPCRNTPQNR 63
           MG +  V VL  LL+   +  S  Q   FD+F  ++QW  A C +S     P    P   
Sbjct: 1   MGAKGCVNVLLKLLVFQGLFVSRPQ-EDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAAD 60

Query: 64  FSIHGVWPSLNSGA-SAQCSGTPPYDGRLMTRAIRDRLDQDWPSVVYGINP--TLWSHEW 123
           F IHG+WP+ N G+  + C     +D R     +   L ++WP++    N     W HEW
Sbjct: 61  FGIHGLWPNYNGGSWPSNCDPDSQFD-RSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEW 120

Query: 124 TAHGTCSNSSMNQVQYFTFGLDEFAKYNLRSTLAAYGINPDGKEYSRGDVEAAIFCGTGG 183
             HGTCS S M+Q +YF   L    K NL   L   GINPD   Y+   +  AI  G G 
Sbjct: 121 EKHGTCSESVMDQHEYFENALKLKQKANLLQILKNSGINPDDGFYNLDKITNAIKDGIGF 180

Query: 184 KWAALRCNRNRAGQKQLYEIALCFTKDNRQLMDCP-LNAVTCGSKFLWSSW 228
               + CN++     QL++I +C      + ++CP L   +C S+  +S +
Sbjct: 181 T-PGIECNKDPERNAQLHQIYICVDTSGTEFIECPVLPRGSCPSQIQFSKF 228

[Close]

BLAST of Lag0026659 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 104.8 bits (260), Expect = 1.0e-22
Identity = 68/229 (29.69%), Postives = 109/229 (47.60%), Query Frame = 0


Query: 10  VLVLSMLLL--LISTSEGQVNQFDYFQLIIQWQPAACSSST-LPSPCRNTPQNRFSIHGV 69
           ++++S+L+L  L+ +S      F++F  +  W  A C S      P +    + F IHG+
Sbjct: 4   IIIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFIIHGL 63

Query: 70  WPSLNSGA-SAQCSGTPPYDGRLMTRAI--RDRLDQDWPSVVYGINPT-LWSHEWTAHGT 129
           WP  N+G   A C  T  +D   ++  +   ++   +W       N T LW HEW  HGT
Sbjct: 64  WPQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKHGT 123

Query: 130 CSNSSMNQVQYFTFGLDEFAKYNLRSTLAAYGINPDGKEYSRGDVEAAIFCGTGGKWAAL 189
           C  S  +Q  YF   L    K +L + L   GI P+   YS  +++ AI C  G     +
Sbjct: 124 CVQSIFDQHSYFRTNLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFA-PGI 183

Query: 190 RCNRNRAGQKQLYEIALCFTKDNRQLMDCP-LNAVTCGSKFLWSSWSNR 231
            CN +  G KQL++I +C     ++ ++CP +   +C SK  + S   R
Sbjct: 184 ECNEDVKGNKQLFQIYICLDNYAKEFVECPYVPDKSCASKIKFPSLPER 231

[Close]

BLAST of Lag0026659 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 77.8 bits (190), Expect = 1.3e-14
Identity = 53/190 (27.89%), Postives = 88/190 (46.32%), Query Frame = 0


Query: 29  QFDYFQLIIQWQPAACSSS----TLPSPCRNT-PQNRFSIHGVWPSLNSGASAQCSGTPP 88
           +FDYF L +QW    C  +    +  + CR +    +F+IHG+WP  N G+   C     
Sbjct: 31  EFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSD 90

Query: 89  YDGRLMTRAIRDRLDQDWPSVVYGI-------NPTLWSHEWTAHGTCSNSSM-NQVQYFT 148
           +  + ++  + D L++ WPS+  G          + W HEW  HGTCS+    ++  YF 
Sbjct: 91  FKEKEIS-TLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFL 150

Query: 149 FGLDEFAKYNLRSTLAAYG-INPDGKEYSRGDVEAAIFCGTGGKWAALRCNRNRAGQKQL 205
             L+ + K+N+   L   G +  + ++Y  G +  AI          + C R+      +
Sbjct: 151 TTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAI-QNAFHITPEVVCKRD-----AI 210

The following BLAST results are available for this feature:

BLAST of Lag0026659 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903348.1	3.7e-83	67.41	ribonuclease MC-like [Benincasa hispida]	[more]
XP_022158939.1	7.2e-71	57.96	ribonuclease MC-like [Momordica charantia]	[more]
XP_022156540.1	4.1e-66	57.46	ribonuclease MC-like [Momordica charantia]	[more]
XP_022159354.1	1.1e-60	52.44	ribonuclease MC-like [Momordica charantia]	[more]
AEM42996.1	4.0e-53	48.26	S-RNase [Siraitia grosvenorii]	[more]

BLAST of Lag0026659 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	3.9e-27	32.65	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P42813	6.6e-27	31.58	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P42815	5.2e-24	33.51	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
Q40965	6.4e-22	31.55	Ribonuclease S-2 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]
Q40966	1.4e-21	29.13	Ribonuclease S-4 OS=Pyrus pyrifolia OX=3767 PE=1 SV=2	[more]

BLAST of Lag0026659 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E0X1	3.5e-71	57.96	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1	[more]
A0A6J1DQK2	2.0e-66	57.46	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1	[more]
A0A6J1E266	5.6e-61	52.44	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1	[more]
K7NBR4	1.9e-53	48.26	S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1	[more]
A0A6J1D7Q5	1.4e-51	48.02	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1	[more]

BLAST of Lag0026659 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	4.7e-28	31.58	ribonuclease 1	[more]
AT1G26820.1	3.7e-25	33.51	ribonuclease 3	[more]
AT1G14220.1	4.9e-25	32.47	Ribonuclease T2 family protein	[more]
AT1G14210.1	1.0e-22	29.69	Ribonuclease T2 family protein	[more]
AT2G39780.1	1.3e-14	27.89	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 29..222 e-value: 2.0E-50 score: 173.4
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 30..223
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 30..211 e-value: 7.3E-38 score: 130.6
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 15..213
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 15..213
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 30..212 e-value: 1.27482E-52 score: 167.124

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0026659.1	Lag0026659.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0016070	RNA metabolic process
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding