Lag0026590 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0026590
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Cytochrome P450, putative
Location	chr10: 39435499 .. 39436452 (-)
RNA-Seq Expression	Lag0026590
Synteny	Lag0026590

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGCCATGCCCAGCATCCTTGACAACACCTCTCCTTATCAATTTTGGTGCTTTTGGTTCATCATTGCCATCCTCCTCCATCTCTTCCTCAAAAAACATCTCCCAAAACCTAAACCCTCATCTTCTACCATCCTCGCGCCGCCCGCCCTCCCTCTCATCGGTCACCTCCACCTCCTGACCCCGGTTCTAGTTACCTCCTTCCAAGCGCTAGCTCGCCGCTACGGCCCCCTCATCGAGCTCCGACTTGGCGCATCAAAGTGCGTCATTGTATCTAATGCCGCAGTCGCCAAAGAGATCCTCAAAACTCACGAACACAACTTCCTATCTCGACCCGAGTTCGGCGCATCCGAGTACTTCATCTACCAAGGGTCCAGATTCGTCCTAGCCCCCTATGGACCCTACTGGCGATTCATGAAGAAACTAACTATGACACGGCTTCTCTCGCCGTCGCAGTTGGCAGCCTCCACCGCCATCCGCGGCGACGAGGTGGCGAAGCTAGTGGAGAGAATAGAGGCAAATTCAAGAGAAAAGAAGCCCTCGGATTTGAGATTGGAATTTACCACTTTGACAAACAACATCATCTCGAGGATGCTTTTGAGCACAAGGTGCTCTGGTGGGAAAGATGAGGCCCAAGAGATTAAGGACTTGGTGTGGAGTATCAATAGGCTGGCTGGGAAGTTGAGTATGGGGGACATTTTGGGACCTTTGAAAGTGCTTGATTTCTCTGGAAATGGAAGGAAGTTTGTGAGAACTATGAACAGATTTGATGGGTTGGTGGAGAAGATAATGAAGGAGCATGAAGACAACAACAAGGCTGCCATTAATGGCGGTGGAGATGTGGAGAGAAGAAGAGATTTGTTGGATATCTTGCTGGAGATTTACAATGATCCAGATGCTGAGATGAAGATAACCAGAAAAGATATCAAGTCCTTCTTGCTTGTGAGTTTTTAA

mRNA sequence

ATGGAGGCCATGCCCAGCATCCTTGACAACACCTCTCCTTATCAATTTTGGTGCTTTTGGTTCATCATTGCCATCCTCCTCCATCTCTTCCTCAAAAAACATCTCCCAAAACCTAAACCCTCATCTTCTACCATCCTCGCGCCGCCCGCCCTCCCTCTCATCGGTCACCTCCACCTCCTGACCCCGGTTCTAGTTACCTCCTTCCAAGCGCTAGCTCGCCGCTACGGCCCCCTCATCGAGCTCCGACTTGGCGCATCAAAGTGCGTCATTGTATCTAATGCCGCAGTCGCCAAAGAGATCCTCAAAACTCACGAACACAACTTCCTATCTCGACCCGAGTTCGGCGCATCCGAGTACTTCATCTACCAAGGGTCCAGATTCGTCCTAGCCCCCTATGGACCCTACTGGCGATTCATGAAGAAACTAACTATGACACGGCTTCTCTCGCCGTCGCAGTTGGCAGCCTCCACCGCCATCCGCGGCGACGAGGTGGCGAAGCTAGTGGAGAGAATAGAGGCAAATTCAAGAGAAAAGAAGCCCTCGGATTTGAGATTGGAATTTACCACTTTGACAAACAACATCATCTCGAGGATGCTTTTGAGCACAAGGTGCTCTGGTGGGAAAGATGAGGCCCAAGAGATTAAGGACTTGGTGTGGAGTATCAATAGGCTGGCTGGGAAGTTGAGTATGGGGGACATTTTGGGACCTTTGAAAGTGCTTGATTTCTCTGGAAATGGAAGGAAGTTTGTGAGAACTATGAACAGATTTGATGGGTTGGTGGAGAAGATAATGAAGGAGCATGAAGACAACAACAAGGCTGCCATTAATGGCGGTGGAGATGTGGAGAGAAGAAGAGATTTGTTGGATATCTTGCTGGAGATTTACAATGATCCAGATGCTGAGATGAAGATAACCAGAAAAGATATCAAGTCCTTCTTGCTTGTGAGTTTTTAA

Coding sequence (CDS)

ATGGAGGCCATGCCCAGCATCCTTGACAACACCTCTCCTTATCAATTTTGGTGCTTTTGGTTCATCATTGCCATCCTCCTCCATCTCTTCCTCAAAAAACATCTCCCAAAACCTAAACCCTCATCTTCTACCATCCTCGCGCCGCCCGCCCTCCCTCTCATCGGTCACCTCCACCTCCTGACCCCGGTTCTAGTTACCTCCTTCCAAGCGCTAGCTCGCCGCTACGGCCCCCTCATCGAGCTCCGACTTGGCGCATCAAAGTGCGTCATTGTATCTAATGCCGCAGTCGCCAAAGAGATCCTCAAAACTCACGAACACAACTTCCTATCTCGACCCGAGTTCGGCGCATCCGAGTACTTCATCTACCAAGGGTCCAGATTCGTCCTAGCCCCCTATGGACCCTACTGGCGATTCATGAAGAAACTAACTATGACACGGCTTCTCTCGCCGTCGCAGTTGGCAGCCTCCACCGCCATCCGCGGCGACGAGGTGGCGAAGCTAGTGGAGAGAATAGAGGCAAATTCAAGAGAAAAGAAGCCCTCGGATTTGAGATTGGAATTTACCACTTTGACAAACAACATCATCTCGAGGATGCTTTTGAGCACAAGGTGCTCTGGTGGGAAAGATGAGGCCCAAGAGATTAAGGACTTGGTGTGGAGTATCAATAGGCTGGCTGGGAAGTTGAGTATGGGGGACATTTTGGGACCTTTGAAAGTGCTTGATTTCTCTGGAAATGGAAGGAAGTTTGTGAGAACTATGAACAGATTTGATGGGTTGGTGGAGAAGATAATGAAGGAGCATGAAGACAACAACAAGGCTGCCATTAATGGCGGTGGAGATGTGGAGAGAAGAAGAGATTTGTTGGATATCTTGCTGGAGATTTACAATGATCCAGATGCTGAGATGAAGATAACCAGAAAAGATATCAAGTCCTTCTTGCTTGTGAGTTTTTAA

Protein sequence

MEAMPSILDNTSPYQFWCFWFIIAILLHLFLKKHLPKPKPSSSTILAPPALPLIGHLHLLTPVLVTSFQALARRYGPLIELRLGASKCVIVSNAAVAKEILKTHEHNFLSRPEFGASEYFIYQGSRFVLAPYGPYWRFMKKLTMTRLLSPSQLAASTAIRGDEVAKLVERIEANSREKKPSDLRLEFTTLTNNIISRMLLSTRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILGPLKVLDFSGNGRKFVRTMNRFDGLVEKIMKEHEDNNKAAINGGGDVERRRDLLDILLEIYNDPDAEMKITRKDIKSFLLVSF

Homology

[Close]

BLAST of Lag0026590 vs. NCBI nr
Match: XP_038905529.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Benincasa hispida])

HSP 1 Score: 517.3 bits (1331), Expect = 9.3e-143
Identity = 266/322 (82.61%), Postives = 284/322 (88.20%), Query Frame = 0


Query: 1   MEAMPSILDN-TSPYQFWCFWFIIAILLHLFLKKHLPKPKPSS----STILAPPALPLIG 60
           M+A+ +I DN TS  QFWCFWFIIA LLHLFLKKHLPKPKPSS    +   +PPALPLIG
Sbjct: 17  MKAITAIFDNTTSTSQFWCFWFIIAFLLHLFLKKHLPKPKPSSLGGPTPPPSPPALPLIG 76

Query: 61  HLHLLTPVLVTSFQALARRYGPLIELRLGASKCVIVSNAAVAKEILKTHEHNFLSRPEFG 120
           HLHLLTPVLVTSFQALARRYGPLIELRLGASKCV+VSNAAVAKEILKTHEHNFLSRPEFG
Sbjct: 77  HLHLLTPVLVTSFQALARRYGPLIELRLGASKCVVVSNAAVAKEILKTHEHNFLSRPEFG 136

Query: 121 ASEYFIYQGSRFVLAPYGPYWRFMKKLTMTRLLSPSQLAASTAIRGDEVAKLVERIEANS 180
           ASEYFIY+GSRFV+A YGPYWRFMKKLTMTRLLSP QLA STAIR DE+AKL+ERIE NS
Sbjct: 137 ASEYFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIAKLMERIEGNS 196

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILG 240
           RE KP D+RLEFTTLTNNIISRM+LSTRC GGKDEAQEIKDL W IN LAGKLS+GDILG
Sbjct: 197 RENKPIDMRLEFTTLTNNIISRMVLSTRCCGGKDEAQEIKDLAWRINMLAGKLSLGDILG 256

Query: 241 PLKVLDFSGNGRKFVRTMNRFDGLVEKIMKEHEDNNKAAINGGGDVERRRDLLDILLEIY 300
           PLKV DFSGNG+KFV+T+ RFD LVEKIMKEHE    AA NG GD ERRRDLLDILLEIY
Sbjct: 257 PLKVFDFSGNGKKFVKTLKRFDELVEKIMKEHE----AANNGDGDEERRRDLLDILLEIY 316

Query: 301 NDPDAEMKITRKDIKSFLLVSF 318
            DP+AEMKIT+ DIKSFLL  F
Sbjct: 317 KDPNAEMKITKNDIKSFLLDLF 334

[Close]

BLAST of Lag0026590 vs. NCBI nr
Match: XP_023528514.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 495.7 bits (1275), Expect = 2.9e-136
Identity = 256/322 (79.50%), Postives = 276/322 (85.71%), Query Frame = 0


Query: 1   MEAMPSILDN-TSPYQFWCFWFIIAILLHLFLKKHLPKPKPSSSTILA----PPALPLIG 60
           M  M +I  N T P QFWCFWFI A+LLH FLKK LP PK S     A    PPALPLIG
Sbjct: 1   MNTMATIFHNITFPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60

Query: 61  HLHLLTPVLVTSFQALARRYGPLIELRLGASKCVIVSNAAVAKEILKTHEHNFLSRPEFG 120
           HLHLLTPVLVTSFQ LARRYG LIELRLGASKCV+VS A VAKE+LKTHEHNFLSRPEFG
Sbjct: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120

Query: 121 ASEYFIYQGSRFVLAPYGPYWRFMKKLTMTRLLSPSQLAASTAIRGDEVAKLVERIEANS 180
           ASE+FIY+GSRFV+APYGPYWRFMKKLTMTRLLSP QLA ++AIR DEV KLVERIEANS
Sbjct: 121 ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANS 180

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILG 240
           REKKPSDLRLEFTTLTNNIISRMLLSTRC  G DEAQE++DL+W INRLAGKLS+GDILG
Sbjct: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240

Query: 241 PLKVLDFSGNGRKFVRTMNRFDGLVEKIMKEHEDNNKAAINGGGDVERRRDLLDILLEIY 300
           P KVLDFSGNG+KFVRTM RFDGLVE+IMKEHE    AAINGGGD +R+ D+LDILLE +
Sbjct: 241 PFKVLDFSGNGKKFVRTMKRFDGLVERIMKEHE----AAINGGGDPDRKLDVLDILLECH 300

Query: 301 NDPDAEMKITRKDIKSFLLVSF 318
           NDP+AEM ITRKDIKSFLL  F
Sbjct: 301 NDPNAEMNITRKDIKSFLLDLF 318

[Close]

BLAST of Lag0026590 vs. NCBI nr
Match: KAA0043713.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Cucumis melo var. makuwa] >TYK25421.1 3,9-dihydroxypterocarpan 6A-monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 490.0 bits (1260), Expect = 1.6e-134
Identity = 255/322 (79.19%), Postives = 278/322 (86.34%), Query Frame = 0


Query: 1   MEAMPSILDN-TSPYQFWCFWFIIAILLHLFLKKHLPKPKPSSSTIL----APPALPLIG 60
           M+AM +I DN TSP QFW FWFI A+LLHL LKK L K KPSS        +PPALPLIG
Sbjct: 1   MKAMTTIFDNTTSPSQFWSFWFITALLLHLLLKKILTKSKPSSPNTTKPPPSPPALPLIG 60

Query: 61  HLHLLTPVLVTSFQALARRYGPLIELRLGASKCVIVSNAAVAKEILKTHEHNFLSRPEFG 120
           HLHLLTPV+VTSFQ LARRYGPLIE+RLGASKCVIVS AAVAKEILKTHEHNFLSRPEFG
Sbjct: 61  HLHLLTPVIVTSFQTLARRYGPLIEIRLGASKCVIVSTAAVAKEILKTHEHNFLSRPEFG 120

Query: 121 ASEYFIYQGSRFVLAPYGPYWRFMKKLTMTRLLSPSQLAASTAIRGDEVAKLVERIEANS 180
           ASEYFIY+GSRFV+A YGPYWRFMKKLTMTRLLSP QLA STAIR DE+ KLVERIEA+S
Sbjct: 121 ASEYFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIVKLVERIEASS 180

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILG 240
           RE KPSD+RLEFTTLTNNIISRMLLSTRC GGKDEA+EIK+L W IN LAGKLS+GDILG
Sbjct: 181 REGKPSDMRLEFTTLTNNIISRMLLSTRCCGGKDEAKEIKELAWRINMLAGKLSLGDILG 240

Query: 241 PLKVLDFSGNGRKFVRTMNRFDGLVEKIMKEHEDNNKAAINGGGDVERRRDLLDILLEIY 300
            LKV DFSGNG+KFV T+ +FDGLVE+IMKEHE    AAI+  GD ER++DLLDILLEIY
Sbjct: 241 ALKVFDFSGNGKKFVETLKKFDGLVERIMKEHE----AAID-SGDEERKKDLLDILLEIY 300

Query: 301 NDPDAEMKITRKDIKSFLLVSF 318
           NDP+A+MKITR DIKSFLL  F
Sbjct: 301 NDPNADMKITRTDIKSFLLDLF 317

[Close]

BLAST of Lag0026590 vs. NCBI nr
Match: XP_022934356.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita moschata])

HSP 1 Score: 488.8 bits (1257), Expect = 3.5e-134
Identity = 254/322 (78.88%), Postives = 274/322 (85.09%), Query Frame = 0


Query: 1   MEAMPSILDN-TSPYQFWCFWFIIAILLHLFLKKHLPKPKPSSSTILA----PPALPLIG 60
           M AM +I  N TS   FWCFWFI A+LLH FLKK LP PK S     A    PPALPLIG
Sbjct: 1   MNAMVTIFHNITSLSPFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60

Query: 61  HLHLLTPVLVTSFQALARRYGPLIELRLGASKCVIVSNAAVAKEILKTHEHNFLSRPEFG 120
           HLHLLTPVLVTSFQ LARRYG LIELRLGASKCV+VS A VAKE+LKTHEHNFLSRPEFG
Sbjct: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120

Query: 121 ASEYFIYQGSRFVLAPYGPYWRFMKKLTMTRLLSPSQLAASTAIRGDEVAKLVERIEANS 180
           ASEYFIY+GSRFV+APYGPYWRFMKKLTMTRLLSP QLA + AIR DEV KLVERIEANS
Sbjct: 121 ASEYFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIAAAIRRDEVGKLVERIEANS 180

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILG 240
           REKKPSDLRLEFTTLTNNIISRMLLSTRC  G DEAQE++DL+W INRLAGKLS+GDILG
Sbjct: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240

Query: 241 PLKVLDFSGNGRKFVRTMNRFDGLVEKIMKEHEDNNKAAINGGGDVERRRDLLDILLEIY 300
           PLKVLDFSGNG+KFV TM RFDGLVE+IMKEHE    AA+NGG D +R+ D+LDILLE +
Sbjct: 241 PLKVLDFSGNGKKFVTTMKRFDGLVERIMKEHE----AAVNGGRDPDRKLDVLDILLECH 300

Query: 301 NDPDAEMKITRKDIKSFLLVSF 318
           NDP+AEM I+RKDIKSFLL  F
Sbjct: 301 NDPNAEMNISRKDIKSFLLDLF 318

[Close]

BLAST of Lag0026590 vs. NCBI nr
Match: KAG7017572.1 (3,9-dihydroxypterocarpan 6A-monooxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 488.8 bits (1257), Expect = 3.5e-134
Identity = 254/322 (78.88%), Postives = 274/322 (85.09%), Query Frame = 0


Query: 1   MEAMPSILDN-TSPYQFWCFWFIIAILLHLFLKKHLPKPKPSSSTILA----PPALPLIG 60
           M AM +I  N TS   FWCFWFI A+LLH FLKK LP PK S     A    PPALPLIG
Sbjct: 1   MNAMVTIFHNITSLSPFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60

Query: 61  HLHLLTPVLVTSFQALARRYGPLIELRLGASKCVIVSNAAVAKEILKTHEHNFLSRPEFG 120
           HLHLLTPVLVTSFQ LARRYG LIELRLGASKCV+VS A VAKE+LKTHEHNFLSRPEFG
Sbjct: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120

Query: 121 ASEYFIYQGSRFVLAPYGPYWRFMKKLTMTRLLSPSQLAASTAIRGDEVAKLVERIEANS 180
           ASEYFIY+GSRFV+APYGPYWRFMKKLTMTRLLSP QLA + AIR DEV KLVERIEANS
Sbjct: 121 ASEYFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIAAAIRRDEVGKLVERIEANS 180

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILG 240
           REKKPSDLRLEFTTLTNNIISRMLLSTRC  G DEAQE++DL+W INRLAGKLS+GDILG
Sbjct: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240

Query: 241 PLKVLDFSGNGRKFVRTMNRFDGLVEKIMKEHEDNNKAAINGGGDVERRRDLLDILLEIY 300
           PLKVLDFSGNG+KFV TM RFDGLVE+IMKEHE    AA+NGG D +R+ D+LDILLE +
Sbjct: 241 PLKVLDFSGNGKKFVTTMKRFDGLVERIMKEHE----AAVNGGRDPDRKLDVLDILLECH 300

Query: 301 NDPDAEMKITRKDIKSFLLVSF 318
           NDP+AEM I+RKDIKSFLL  F
Sbjct: 301 NDPNAEMNISRKDIKSFLLDLF 318

[Close]

BLAST of Lag0026590 vs. ExPASy Swiss-Prot
Match: Q9XGT9 (Cytochrome P450 93B2 OS=Gerbera hybrida OX=18101 GN=CYP93B2 PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.6e-49
Identity = 118/307 (38.44%), Postives = 175/307 (57.00%), Query Frame = 0


Query: 10  NTSPYQFWCFWFIIAILLHLFLKKHLPKPKPSSSTIL--APPALPLIGHLHLLTPVLVTS 69
           NT    F  F+F   + L+      LP  +  +   L  +PP+ P+IGHLH L P++  S
Sbjct: 2   NTLQLIFLLFFFPTLLFLYC-----LPYKRNQNHRRLPPSPPSFPIIGHLHHLGPLIHQS 61

Query: 70  FQALARRYGPLIELRLGASKCVIVSNAAVAKEILKTHEHNFLSRPEFGASEYFIYQGSRF 129
           F AL+ RYG LI LRLG+  CV+VS   +AK+ LKT+E  F SR    A ++  Y G  F
Sbjct: 62  FHALSTRYGSLIHLRLGSVPCVVVSTPDLAKDFLKTNELAFSSRKHSLAIDHITY-GVAF 121

Query: 130 VLAPYGPYWRFMKKLTMTRLLSPSQLAASTAIRGDEVAKLVERIEANSREKKPSDLRLEF 189
             APYG YW+F+KKL    LL    L+    IR  E+ +L+  +   SR K+  +L  E 
Sbjct: 122 AFAPYGTYWKFIKKLFTVELLGTQNLSHFLPIRTHEIRELLRTLMVKSRAKERVNLTEEL 181

Query: 190 TTLTNNIISRMLLSTRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILGPLKVLDFSGNGR 249
             LTNN+IS+M++S RCSG   EA E K+LV  + ++ G+ ++ D +   K +D  G  +
Sbjct: 182 LKLTNNVISQMMMSIRCSGTNSEADEAKNLVREVTKIFGQFNVSDFIWFCKNIDLQGFKK 241

Query: 250 KFVRTMNRFDGLVEKIMKEHEDNNKAAINGGGDVERRRDLLDILLEIYNDPDAEMKITRK 309
           ++  T  R+D L+E+I+   E+N +      G+    +D LD+LL++  D  AE+KITR 
Sbjct: 242 RYEGTHRRYDALLERIIMGREENRRRGKIKDGE---GKDFLDMLLDVLEDGKAEIKITRD 299

Query: 310 DIKSFLL 315
            IK+ +L
Sbjct: 302 HIKALIL 299

[Close]

BLAST of Lag0026590 vs. ExPASy Swiss-Prot
Match: Q42798 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 4.8e-49
Identity = 110/296 (37.16%), Postives = 173/296 (58.45%), Query Frame = 0


Query: 25  ILLHLFLKKHLPKPKPSSSTILAPPALPLIGHLHLLTPVLVTSFQALARRYGPLIELRLG 84
           +  ++  +K   K  P S     P ALP+IGHLHL++P+    F  L+ R+GP+++L LG
Sbjct: 15  VFAYILWRKQSKKNLPPS-----PKALPIIGHLHLVSPIPHQDFYKLSTRHGPIMQLFLG 74

Query: 85  ASKCVIVSNAAVAKEILKTHEHNFLSRPEFGASEYFIYQGSR---FVLAPYGPYWRFMKK 144
           +  CV+ S A  AKE LKTHE NF +RP    +   +   S+   F  AP+GPYW+FMKK
Sbjct: 75  SVPCVVASTAEAAKEFLKTHEINFSNRPGQNVAVKGLAYDSQDFLFAFAPFGPYWKFMKK 134

Query: 145 LTMTRLLSPSQLAASTAIRGDEVAKLVERIEANSREKKPSDLRLEFTTLTNNIISRMLLS 204
           L M+ LLS   +     +R  E  + + R+       +  D   E  TL+NNI+SRM LS
Sbjct: 135 LCMSELLSGRMMDQFLPVRQQETKRFISRVFRKGVAGEAVDFGDELMTLSNNIVSRMTLS 194

Query: 205 TRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILGPLKVLDFSGNGRKFVRTMNRFDGLVE 264
            + S   ++A+E+K LV +I  L GK ++ D +  LK  D  G  RK   T +RFD +V+
Sbjct: 195 QKTSENDNQAEEMKKLVSNIAELMGKFNVSDFIWYLKPFDLQGFNRKIKETRDRFDVVVD 254

Query: 265 KIMKEHEDNNKAAINGGGDVERRRDLLDILLEIYNDPDAEMKITRKDIKSFLLVSF 318
            I+K+ ++  +      G  ++ +D+LD+LL+++ D +AE+K+ +K+IK+F++  F
Sbjct: 255 GIIKQRQEERRKN-KETGTAKQFKDMLDVLLDMHEDENAEIKLDKKNIKAFIMDIF 304

[Close]

BLAST of Lag0026590 vs. ExPASy Swiss-Prot
Match: E9KBR8 (Cytochrome P450 93B16 OS=Glycine max OX=3847 GN=CYP93B16 PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.8e-48
Identity = 111/300 (37.00%), Postives = 177/300 (59.00%), Query Frame = 0


Query: 21  FIIAILLHLFLKKHLPKPKPSSSTILAPPALPLIGHLHLLTPVLVTSFQALARRYGPLIE 80
           FI A LL L   +   KPK       +PPA+P+IGHLHLL P++  SF+ L+ RYGPL+ 
Sbjct: 14  FISASLLKLLFVRE-NKPKAHLKNPPSPPAIPIIGHLHLLKPLIHHSFRDLSLRYGPLLS 73

Query: 81  LRLGASKCVIVSNAAVAKEILKTHEHNFLSRPEFGASEYFIYQGSRFVLAPYGPYWRFMK 140
           LR+G+ K ++ S  ++A+E LKT+E  + SR    A     Y  + F  APY  YW+FMK
Sbjct: 74  LRIGSVKFIVASTPSLAQEFLKTNELTYSSRKMNMAINMVTYHNATFAFAPYDTYWKFMK 133

Query: 141 KLTMTRLLSPSQLAASTAIRGDEVAKLVERIEANSREKKPSDLRLEFTTLTNNIISRMLL 200
           KL+ T LL    L     IR  EV  +++ +   S+ ++  +L     +L+NN+IS+M+L
Sbjct: 134 KLSTTELLGNKTLGHFLPIRTREVHDIIQFLFHKSKAQESVNLTEALLSLSNNVISQMML 193

Query: 201 STRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILGPLKVLDFSGNGRKFVRTMNRFDGLV 260
           S + SG   +A++ + LV  + ++ G+ ++ D LG  K LD  G  ++ +    R+D L+
Sbjct: 194 SIKSSGTDSQAEQARTLVREVTQIFGEFNVSDFLGFCKNLDLQGFRKRALDIHKRYDALL 253

Query: 261 EKIMKEHED-NNKAAING--GGDVERRRDLLDILLEIYNDPDAEMKITRKDIKSFLLVSF 318
           EKI+ + E+   K+ ++G   GD E+ +D LDILL++    + E+++TR  +KS +L  F
Sbjct: 254 EKIISDREELRRKSKVDGCEDGDDEKVKDFLDILLDVAEQKECEVQLTRNHVKSLILDYF 312

[Close]

BLAST of Lag0026590 vs. ExPASy Swiss-Prot
Match: Q9XHC6 (Beta-amyrin 24-hydroxylase OS=Glycine max OX=3847 GN=CYP93E1 PE=1 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 9.9e-47
Identity = 109/299 (36.45%), Postives = 164/299 (54.85%), Query Frame = 0


Query: 20  WFIIAILLHLFLKKHLPKPKPSSSTILAPPALPLIGHLHLLTPVLVTSFQALARRYGPLI 79
           WFI  IL+    KK      P       P ++PL+GH   L  +L  +   L+ RYGPLI
Sbjct: 14  WFISTILIRSIFKKPQRLRLPPG----PPISIPLLGHAPYLRSLLHQALYKLSLRYGPLI 73

Query: 80  ELRLGASKCVIVSNAAVAKEILKTHEHNFLSRPEFGASEYFIYQGSRFVLAPYGPYWRFM 139
            + +G+   V+ S+A  AK+ILKT E  F +RP   ASE   Y  + +   PYG YWRF+
Sbjct: 74  HVMIGSKHVVVASSAETAKQILKTSEEAFCNRPLMIASESLTYGAADYFFIPYGTYWRFL 133

Query: 140 KKLTMTRLLSPSQLAASTAIRGDEVAKLVER-IEANSREKKPSDLRLEFTTLTNNIISRM 199
           KKL MT LLS   L     IR  EV   ++R +E +        +R E  T TNNII+RM
Sbjct: 134 KKLCMTELLSGKTLEHFVRIRESEVEAFLKRMMEISGNGNYEVVMRKELITHTNNIITRM 193

Query: 200 LLSTRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILGPLKVLDFSGNGRKFVRTMNRFDG 259
           ++  + +   DE   ++ +V  +  L G  ++GD++G ++ LD  G G+K + T ++ D 
Sbjct: 194 IMGKKSNAENDEVARLRKVVREVGELLGAFNLGDVIGFMRPLDLQGFGKKNMETHHKVDA 253

Query: 260 LVEKIMKEHEDNNKAAINGGGDVERRRDLLDILLEIYNDPDAEMKITRKDIKSFLLVSF 318
           ++EK+++EHE+   A      D +R++DL DILL +     A+ K+TR+  K+F L  F
Sbjct: 254 MMEKVLREHEE---ARAKEDADSDRKKDLFDILLNLIEADGADNKLTRESAKAFALDMF 305

[Close]

BLAST of Lag0026590 vs. ExPASy Swiss-Prot
Match: O81973 (Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.4e-45
Identity = 108/283 (38.16%), Postives = 165/283 (58.30%), Query Frame = 0


Query: 39  KPSSSTILAPPA---LPLIGHLHLLTPVLVTSFQALARRYGPLIELRLGASKCVIVSNAA 98
           K + +  L PP+   LP+IGHLHLL+P     F  L+ RYGP+I L LG+  CV+ S A 
Sbjct: 23  KQNKNKTLLPPSPMPLPIIGHLHLLSPTPHQDFHKLSLRYGPIIHLFLGSVPCVVASTAE 82

Query: 99  VAKEILKTHEHNFLSRP-EFGASEYFIYQGSRFVLAPYGPYWRFMKKLTMTRLLSPSQLA 158
            AKE LKTHE  F +RP    A E   Y    F+ APYGPYW+FMKKL M+ LL    L 
Sbjct: 83  AAKEFLKTHEPAFSNRPANTVAVETLTYGFQDFLFAPYGPYWKFMKKLCMSELLGGHMLD 142

Query: 159 ASTAIRGDEVAKLVERIEANSREKKPSDLRLEFTTLTNNIISRMLLS-TRCSGGKDEAQE 218
               +R  E  K ++R+       +  D   EF TL+NNI+SRM++S T  +  ++E +E
Sbjct: 143 QFLPVRQXETKKFIKRVLQKGISGEAVDFGGEFITLSNNIVSRMIVSQTSTTEDENEVEE 202

Query: 219 IKDLVWSINRLAGKLSMGDILGPLKVLDFSGNGRKFVRTMNRFDGLVEKIMKEHEDNNKA 278
           ++ LV     L+GK ++ D +  LK  D  G  ++  +  + FD ++++I+K+ E+  + 
Sbjct: 203 MRKLVKDAAELSGKFNISDFVSFLKRFDLQGFNKRLEKIRDCFDTVLDRIIKQREEERR- 262

Query: 279 AINGGGDVERR--RDLLDILLEIYNDPDAEMKITRKDIKSFLL 315
             N    V +R  +D+LD+L +I  D  +E+K+ +++IK+F+L
Sbjct: 263 --NKNETVGKREFKDMLDVLFDISEDESSEIKLNKENIKAFIL 302

[Close]

BLAST of Lag0026590 vs. ExPASy TrEMBL
Match: A0A5D3DP65 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005640 PE=3 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 7.7e-135
Identity = 255/322 (79.19%), Postives = 278/322 (86.34%), Query Frame = 0


Query: 1   MEAMPSILDN-TSPYQFWCFWFIIAILLHLFLKKHLPKPKPSSSTIL----APPALPLIG 60
           M+AM +I DN TSP QFW FWFI A+LLHL LKK L K KPSS        +PPALPLIG
Sbjct: 1   MKAMTTIFDNTTSPSQFWSFWFITALLLHLLLKKILTKSKPSSPNTTKPPPSPPALPLIG 60

Query: 61  HLHLLTPVLVTSFQALARRYGPLIELRLGASKCVIVSNAAVAKEILKTHEHNFLSRPEFG 120
           HLHLLTPV+VTSFQ LARRYGPLIE+RLGASKCVIVS AAVAKEILKTHEHNFLSRPEFG
Sbjct: 61  HLHLLTPVIVTSFQTLARRYGPLIEIRLGASKCVIVSTAAVAKEILKTHEHNFLSRPEFG 120

Query: 121 ASEYFIYQGSRFVLAPYGPYWRFMKKLTMTRLLSPSQLAASTAIRGDEVAKLVERIEANS 180
           ASEYFIY+GSRFV+A YGPYWRFMKKLTMTRLLSP QLA STAIR DE+ KLVERIEA+S
Sbjct: 121 ASEYFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIVKLVERIEASS 180

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILG 240
           RE KPSD+RLEFTTLTNNIISRMLLSTRC GGKDEA+EIK+L W IN LAGKLS+GDILG
Sbjct: 181 REGKPSDMRLEFTTLTNNIISRMLLSTRCCGGKDEAKEIKELAWRINMLAGKLSLGDILG 240

Query: 241 PLKVLDFSGNGRKFVRTMNRFDGLVEKIMKEHEDNNKAAINGGGDVERRRDLLDILLEIY 300
            LKV DFSGNG+KFV T+ +FDGLVE+IMKEHE    AAI+  GD ER++DLLDILLEIY
Sbjct: 241 ALKVFDFSGNGKKFVETLKKFDGLVERIMKEHE----AAID-SGDEERKKDLLDILLEIY 300

Query: 301 NDPDAEMKITRKDIKSFLLVSF 318
           NDP+A+MKITR DIKSFLL  F
Sbjct: 301 NDPNADMKITRTDIKSFLLDLF 317

[Close]

BLAST of Lag0026590 vs. ExPASy TrEMBL
Match: A0A6J1F1K7 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111441548 PE=3 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 1.7e-134
Identity = 254/322 (78.88%), Postives = 274/322 (85.09%), Query Frame = 0


Query: 1   MEAMPSILDN-TSPYQFWCFWFIIAILLHLFLKKHLPKPKPSSSTILA----PPALPLIG 60
           M AM +I  N TS   FWCFWFI A+LLH FLKK LP PK S     A    PPALPLIG
Sbjct: 1   MNAMVTIFHNITSLSPFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60

Query: 61  HLHLLTPVLVTSFQALARRYGPLIELRLGASKCVIVSNAAVAKEILKTHEHNFLSRPEFG 120
           HLHLLTPVLVTSFQ LARRYG LIELRLGASKCV+VS A VAKE+LKTHEHNFLSRPEFG
Sbjct: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120

Query: 121 ASEYFIYQGSRFVLAPYGPYWRFMKKLTMTRLLSPSQLAASTAIRGDEVAKLVERIEANS 180
           ASEYFIY+GSRFV+APYGPYWRFMKKLTMTRLLSP QLA + AIR DEV KLVERIEANS
Sbjct: 121 ASEYFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIAAAIRRDEVGKLVERIEANS 180

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILG 240
           REKKPSDLRLEFTTLTNNIISRMLLSTRC  G DEAQE++DL+W INRLAGKLS+GDILG
Sbjct: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240

Query: 241 PLKVLDFSGNGRKFVRTMNRFDGLVEKIMKEHEDNNKAAINGGGDVERRRDLLDILLEIY 300
           PLKVLDFSGNG+KFV TM RFDGLVE+IMKEHE    AA+NGG D +R+ D+LDILLE +
Sbjct: 241 PLKVLDFSGNGKKFVTTMKRFDGLVERIMKEHE----AAVNGGRDPDRKLDVLDILLECH 300

Query: 301 NDPDAEMKITRKDIKSFLLVSF 318
           NDP+AEM I+RKDIKSFLL  F
Sbjct: 301 NDPNAEMNISRKDIKSFLLDLF 318

[Close]

BLAST of Lag0026590 vs. ExPASy TrEMBL
Match: A0A6J1J5T8 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111481610 PE=3 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.2e-134
Identity = 255/324 (78.70%), Postives = 276/324 (85.19%), Query Frame = 0


Query: 1   MEAMPSILDN-TSPYQFWCFWFIIAILLHLFLKKHLPKPKPS----SSTILAPPALPLIG 60
           M  M +I  N TSP QFWCFWFI A+LLH FLKK LP PK S     +   +PPALPLIG
Sbjct: 1   MNTMATIFHNITSPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGHAPPPSPPALPLIG 60

Query: 61  HLHLLTPVLVTSFQALARRYGPLIELRLGASKCVIVSNAAVAKEILKTHEHNFLSRPEFG 120
           HLHLLTPVLVTSFQ LARRYG LIELRLGASKCV+VS AAVAKE+LKTHEHNFLSRPEFG
Sbjct: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTAAVAKEVLKTHEHNFLSRPEFG 120

Query: 121 ASEYFIYQGSRFVLAPYGPYWRFMKKLTMTRLLSPSQLAASTAIRGDEVAKLVERIEANS 180
           ASE+FIY+GSRFV+APYGPYWRFMKKLTMTRLLSP QLA + AIR DEV KLVERIEANS
Sbjct: 121 ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIAAAIRRDEVGKLVERIEANS 180

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILG 240
           R KKPSDLRLEFTTLTNNIISRMLLSTRC  G DEAQE++DL+W INRLAGKLS+GDILG
Sbjct: 181 RGKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240

Query: 241 PLKVLDFSGNGRKFVRTMNRFDGLVEKIMKEHEDNNKAAINGGG--DVERRRDLLDILLE 300
           PLKVLDFSGNG+KFV TM RFDGLVE+IMKEHE    AAINGGG  D +R+ D+LDILLE
Sbjct: 241 PLKVLDFSGNGKKFVTTMKRFDGLVERIMKEHE----AAINGGGHPDPDRKLDVLDILLE 300

Query: 301 IYNDPDAEMKITRKDIKSFLLVSF 318
            +NDP AEM I+RKDIKSFLL  F
Sbjct: 301 CHNDPHAEMNISRKDIKSFLLDLF 320

[Close]

BLAST of Lag0026590 vs. ExPASy TrEMBL
Match: A0A1S3B805 (LOW QUALITY PROTEIN: 3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103486788 PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 6.5e-134
Identity = 254/322 (78.88%), Postives = 276/322 (85.71%), Query Frame = 0


Query: 1   MEAMPSILDN-TSPYQFWCFWFIIAILLHLFLKKHLPKPKPSSSTIL----APPALPLIG 60
           M+AM +I DN TSP QFW FWFI A+LLHL LKK L K KPSS        +PPALPLIG
Sbjct: 15  MKAMTTIFDNTTSPSQFWSFWFITALLLHLLLKKILTKSKPSSPNTTKPPPSPPALPLIG 74

Query: 61  HLHLLTPVLVTSFQALARRYGPLIELRLGASKCVIVSNAAVAKEILKTHEHNFLSRPEFG 120
           HLHLLTPV+VTSFQ LARRYGPLIE+RLGASKCVIVS AAVAKEILKTHEHNFLSRPEFG
Sbjct: 75  HLHLLTPVIVTSFQTLARRYGPLIEIRLGASKCVIVSTAAVAKEILKTHEHNFLSRPEFG 134

Query: 121 ASEYFIYQGSRFVLAPYGPYWRFMKKLTMTRLLSPSQLAASTAIRGDEVAKLVERIEANS 180
           ASEYFIY+GSRFV+A YGPYWRFMKKLTMTRLLSP QLA STAIR DE+ KLVERIEA+S
Sbjct: 135 ASEYFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIVKLVERIEASS 194

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILG 240
           RE KPSD+RLEFTTLTNNIISRMLLSTRC GGKDEA+EIK+L W IN LAGKLS+GDILG
Sbjct: 195 REGKPSDMRLEFTTLTNNIISRMLLSTRCCGGKDEAKEIKELAWRINMLAGKLSLGDILG 254

Query: 241 PLKVLDFSGNGRKFVRTMNRFDGLVEKIMKEHEDNNKAAINGGGDVERRRDLLDILLEIY 300
            LKV DFSGNG+KFV T+ +FDGLVE+IMKEHE    AAI+ G   E R+DLLDILLEIY
Sbjct: 255 ALKVFDFSGNGKKFVETLKKFDGLVERIMKEHE----AAIDSGD--EERKDLLDILLEIY 314

Query: 301 NDPDAEMKITRKDIKSFLLVSF 318
           NDP+A+MKITR DIKSFLL  F
Sbjct: 315 NDPNADMKITRTDIKSFLLDLF 330

[Close]

BLAST of Lag0026590 vs. ExPASy TrEMBL
Match: A0A0A0LBD2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G810500 PE=3 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 6.3e-129
Identity = 243/322 (75.47%), Postives = 271/322 (84.16%), Query Frame = 0


Query: 1   MEAMPSILDN-TSPYQFWCFWFIIAILLHLFLKKHLPKPKPSSSTILAPP----ALPLIG 60
           M+AM +I DN TSP QFW F FI  +L HL L+K L K +PSS     PP    ALP IG
Sbjct: 1   MKAMTTIFDNTTSPSQFWSFCFITILLFHLLLRKLLTKSRPSSPDTPKPPPSPSALPFIG 60

Query: 61  HLHLLTPVLVTSFQALARRYGPLIELRLGASKCVIVSNAAVAKEILKTHEHNFLSRPEFG 120
           HLHLLTPVLVTSFQ LARRYGPLIE+RLGASKC++VS A VAKEILKTHE NFLSRPEFG
Sbjct: 61  HLHLLTPVLVTSFQTLARRYGPLIEVRLGASKCIVVSTATVAKEILKTHEGNFLSRPEFG 120

Query: 121 ASEYFIYQGSRFVLAPYGPYWRFMKKLTMTRLLSPSQLAASTAIRGDEVAKLVERIEANS 180
           ASEYFIY+GSRFV+A YGPYWRFMKKLTMTRLLSP QLA STAIR +E+AKLVERIEA+S
Sbjct: 121 ASEYFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSEEIAKLVERIEASS 180

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILG 240
           RE+KP DLRLEFTTLTNNIISRM+LSTRC GGKDEA+EIKDL W IN LAGKLS+GDILG
Sbjct: 181 REEKPLDLRLEFTTLTNNIISRMVLSTRCCGGKDEAKEIKDLAWRINMLAGKLSLGDILG 240

Query: 241 PLKVLDFSGNGRKFVRTMNRFDGLVEKIMKEHEDNNKAAINGGGDVERRRDLLDILLEIY 300
            LKV DFSGNG+KFV+T+ +FD L+E+IMKEHE    AAIN G D ER++DLLDILLEIY
Sbjct: 241 ALKVFDFSGNGKKFVKTLKKFDELIERIMKEHE----AAINDGEDEERKKDLLDILLEIY 300

Query: 301 NDPDAEMKITRKDIKSFLLVSF 318
           NDP+A+ K+TR DIKSFLL  F
Sbjct: 301 NDPNADFKLTRNDIKSFLLDLF 318

[Close]

BLAST of Lag0026590 vs. TAIR 10
Match: AT2G42250.1 (cytochrome P450, family 712, subfamily A, polypeptide 1 )

HSP 1 Score: 308.1 bits (788), Expect = 8.0e-84
Identity = 169/299 (56.52%), Postives = 211/299 (70.57%), Query Frame = 0


Query: 19  FWFIIAILLHLFLKKHLPKPKPSSSTILAPPALPLIGHLHLLTPVLVTSFQALARRYGPL 78
           F F++  L   FLK+       ++    +PPALP IGHLHL+  VL  SFQ+LA +YGPL
Sbjct: 18  FPFMLYALFKWFLKEQ--GSLAATKLPQSPPALPFIGHLHLIGKVLPVSFQSLAHKYGPL 77

Query: 79  IELRLGASKCVIVSNAAVAKEILKTHEHNFLSRPEFGASEYFIYQGSRFVLAPYGPYWRF 138
           +E+RLGASKCV+VS+++VA+EI K  E NF SRPEFG++EYF Y+GSRFVLA YG YWRF
Sbjct: 78  MEIRLGASKCVVVSSSSVAREIFKEQELNFSSRPEFGSAEYFKYRGSRFVLAQYGDYWRF 137

Query: 139 MKKLTMTRLLSPSQLAASTAIRGDEVAKLVERIEANSREKKPSDLRLEFTTLTNNIISRM 198
           MKKL MT+LL+  QL     IR +E  KLV+ +    RE  P DL  +F   TNN+I RM
Sbjct: 138 MKKLCMTKLLAVPQLEKFADIREEEKLKLVDSVAKCCREGLPCDLSSQFIKYTNNVICRM 197

Query: 199 LLSTRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILGPLKVLDFSGNGRKFVRTMNRFDG 258
            +STRCSG  +EA+EI++LV     LAGK+S+GD+LGPLKV+DFSGNG+K V  M ++D 
Sbjct: 198 AMSTRCSGTDNEAEEIRELVKKSLELAGKISVGDVLGPLKVMDFSGNGKKLVAVMEKYDL 257

Query: 259 LVEKIMKEHEDNNKAAINGGGDVERRRDLLDILLEIYNDPDAEMKITRKDIKSFLLVSF 318
           LVE+IMKE E   KA    G     R+D+LDILLE Y DP AEMKITR D+KSFLL  F
Sbjct: 258 LVERIMKERE--AKAKKKDG----TRKDILDILLETYRDPTAEMKITRNDMKSFLLDVF 308

[Close]

BLAST of Lag0026590 vs. TAIR 10
Match: AT5G06905.1 (cytochrome P450, family 712, subfamily A, polypeptide 2 )

HSP 1 Score: 218.4 bits (555), Expect = 8.3e-57
Identity = 119/297 (40.07%), Postives = 178/297 (59.93%), Query Frame = 0


Query: 21  FIIAILLHLFLKKHLPKPKPSSSTILAPPALPLIGHLHLLTPVLVTSFQALARRYGPLIE 80
           ++   L+ +FL   L            P  LP++GH+HLL   L  S QALA  YGPL+ 
Sbjct: 8   YLFFTLVTIFLLHRLFSSSSRRGLPPGPRGLPVLGHMHLLRSSLPRSLQALAHTYGPLMT 67

Query: 81  LRLGASKCVIVSNAAVAKEILKTHEHNFLSRPEFGASEYFIYQGSRFVLAPYGPYWRFMK 140
           +R+G+ + ++VS++  AK ILKTH+ +F S+  FG  ++ +Y+GS F  APYG YWRFMK
Sbjct: 68  IRIGSLRVLVVSDSDTAKLILKTHDPDFASKFVFGPRQFNVYKGSEFFNAPYGSYWRFMK 127

Query: 141 KLTMTRLLSPSQLAASTAIRGDEVAKLVERIEANSREKKPSDLRLEFTTLTNNIISRMLL 200
           KL MT+L +  QL     IR +E   L+  +   SR  +  DL LEFT LT  I+S+M++
Sbjct: 128 KLCMTKLFAGYQLDRFVDIREEETLALLSTLVERSRNGEACDLGLEFTALTTKILSKMVM 187

Query: 201 STRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILGPLKVLDFSGNGRKFVRTMNRFDGLV 260
             RC    +  +EI+ +V  I   A +    ++ GPL+ LD  GNG+K   ++ R+D LV
Sbjct: 188 GKRCRQNSNIPKEIRKIVSDIMACATRFGFMELFGPLRDLDLFGNGKKLRSSIWRYDELV 247

Query: 261 EKIMKEHEDNNKAAINGGGDVERRRDLLDILLEIYNDPDAEMKITRKDIKSFLLVSF 318
           EKI+KE+E++         + E+ +D++DILL+ YNDP AE+++T   IK F+L  F
Sbjct: 248 EKILKEYEND-----KSNEEEEKDKDIVDILLDTYNDPKAELRLTMNQIKFFILELF 299

[Close]

BLAST of Lag0026590 vs. TAIR 10
Match: AT5G06900.1 (cytochrome P450, family 93, subfamily D, polypeptide 1 )

HSP 1 Score: 188.3 bits (477), Expect = 9.2e-48
Identity = 105/286 (36.71%), Postives = 173/286 (60.49%), Query Frame = 0


Query: 31  LKKHLPKPKPSSSTILAPPALPLIGHLHLLTPVLVTSFQALARRYGPLIELRLGASKCVI 90
           L+  LP P        +P ALP+IGH+HLL P+   +   L+ RYGPL+ L +G+   +I
Sbjct: 28  LRDRLPLPP-------SPTALPIIGHIHLLGPIAHQALHKLSIRYGPLMYLFIGSIPNLI 87

Query: 91  VSNAAVAKEILKTHEHNFLSRPEFGASEYFIYQGSRFVLAPYGPYWRFMKKLTMTRLLSP 150
           VS+A +A EILK++E NFL+RP     +Y  Y  + F  APYG +W+FMK++ M  L S 
Sbjct: 88  VSSAEMANEILKSNELNFLNRPTMQNVDYLTYGSADFFSAPYGLHWKFMKRICMVELFSS 147

Query: 151 SQLAASTAIRGDEVAKLVERIEANSREKKPSDLRLEFTTLTNNIISRMLL--STRCSGGK 210
             L +  ++R +E+ KL+ R+   +  ++  +L  +   LT+NII+RM+       S G 
Sbjct: 148 RALDSFVSVRSEELKKLLIRVLKKAEAEESVNLGEQLKELTSNIITRMMFRKMQSDSDGG 207

Query: 211 DEAQEIKDLVWSINRLAGKLSMGDILGPLKVLDFSGNGRKFVRTMNRFDGLVEKIMKEHE 270
           ++++E+  +V  +N LAG  ++ +    LK LD  G  ++     +++D ++E+IM+EHE
Sbjct: 208 EKSEEVIKMVVELNELAGFFNVSETFWFLKRLDLQGLKKRLKNARDKYDVIIERIMEEHE 267

Query: 271 DNNKAAINGGGDVERRRDLLDILLEIYNDPDAEMKITRKDIKSFLL 315
            + K A          R++LD+LL+IY D +AEMK+TR++IK+F++
Sbjct: 268 SSKKNATG-------ERNMLDVLLDIYEDKNAEMKLTRENIKAFIM 299

[Close]

BLAST of Lag0026590 vs. TAIR 10
Match: AT1G50520.1 (cytochrome P450, family 705, subfamily A, polypeptide 27 )

HSP 1 Score: 171.0 bits (432), Expect = 1.5e-42
Identity = 118/311 (37.94%), Postives = 168/311 (54.02%), Query Frame = 0


Query: 18  CFWFIIAILLHLFLKKHLPKPKPSSSTIL------APPALPLIGHLHLLTPV-LVTSFQA 77
           CF FI+  +  LF        KP    +       +PP+LP+IGHLHLL  V  + SFQ 
Sbjct: 11  CFIFILLGIFSLFCLSAFFFKKPKDPQLQGCGLPPSPPSLPVIGHLHLLLSVPCLKSFQK 70

Query: 78  LARRYGPLIELRLGASKCVIVSNAAVAKEILKTHEHNFLSRP-EFGASEYFIYQGS-RFV 137
           L+ +YGPL+ LR      VIVS+ ++A E+L+T + NF +R  E    E  +  GS  FV
Sbjct: 71  LSSKYGPLLHLRAFNIPIVIVSSGSMANEVLRTQDLNFATRQREVSIMEKSLLFGSFGFV 130

Query: 138 LAPYGPYWRFMKKLTMTRLLSPSQLAASTAIRGDEVAKLVERIEANSREKKPSDLRLEFT 197
            APYG YWRFMKKL +T L     L  +  IR  E+      +   + +K   D+  E  
Sbjct: 131 SAPYGDYWRFMKKLLVTNLFGSHSLEQTRLIREKELKTFRTMLFDKAAKKGTVDVGKEMM 190

Query: 198 TLTNNIISRMLLSTRCSGGKDEAQEIKDLVWSINRLAGKLSMGDILGP-LKVLDFSGNGR 257
            LTNN I RM++  RCS    EA++++DLV     L  K+ + + +G  LK    S   +
Sbjct: 191 KLTNNSICRMIMGRRCSEENSEAEKVEDLVIKSFSLVKKVLIANTVGRLLKKFGISLFEK 250

Query: 258 KFVRTMNRFDGLVEKIMKEH-EDNNKAAINGGGDVERRRDLLDILLEIYNDPDAEMKITR 317
           + +    R+D L+EKI+KEH ED NK         +  RD++D+LLE+  D  AE+KITR
Sbjct: 251 EIMEVSQRYDELLEKIIKEHEEDPNK---------KEDRDMMDVLLEVCADDKAEVKITR 310

[Close]

BLAST of Lag0026590 vs. TAIR 10
Match: AT2G05180.1 (cytochrome P450, family 705, subfamily A, polypeptide 6 )

HSP 1 Score: 168.7 bits (426), Expect = 7.5e-42
Identity = 110/305 (36.07%), Postives = 161/305 (52.79%), Query Frame = 0


Query: 18  CFWFIIAILLHLF-LKKHLPKPKPSSSTIL--APPALPLIGHLHLL-TPVLVTSFQALAR 77
           CF FI+  L  LF       KPK      L  +PP+LP+IGHLHLL +P+   S Q L+ 
Sbjct: 12  CFIFIVLCLFSLFCYSLFFKKPKHPRGCDLPPSPPSLPIIGHLHLLFSPLTHKSLQKLSS 71

Query: 78  RYGPLIELRLGASKCVIVSNAAVAKEILKTHEHNFLSRPEFGASEYFIYQGSRFVLAPYG 137
           +YG L+ LR+     ++VS+A+VA EI + H+ N  SR      E  ++  S  + APYG
Sbjct: 72  KYGHLLHLRIFNVPVILVSSASVAYEIFRAHDVNVSSRGVAAIDESLLFGSSGIITAPYG 131

Query: 138 PYWRFMKKLTMTRLLSPSQLAASTAIRGDEVAKLVERIEANSREKKPSDLRLEFTTLTNN 197
            YW+FMKKL  T+LL P  L +S  IRG+E+ +    +   +R  +  ++  E   L NN
Sbjct: 132 DYWKFMKKLIATKLLRPQSLESSRGIRGEELTRFYNSLLDKARMTESVEISTEAMKLVNN 191

Query: 198 IISRMLLSTRCSGGKDEAQEIKDLVWSINRLAGKLSMGDIL-GPLKVLDFSGNGRKFVRT 257
            + RM +    S    EA++I+ LV     L  K+ +  +L  PLK L  S   ++ +  
Sbjct: 192 TLCRMSMGRSFSEENGEAEKIRGLVGESYALTKKMFLASLLRKPLKKLRISLFEKEIMGV 251

Query: 258 MNRFDGLVEKIMKEHEDNNKAAINGGGDVERRRDLLDILLEIYNDPDAEMKITRKDIKSF 317
            +R D L+E+I+ EHE+       G        D++D+LL    D +AE  ITR  IKSF
Sbjct: 252 SDRLDELLERILVEHEEKLHEEHQG-------TDMMDVLLAASGDENAEYNITRNHIKSF 309

The following BLAST results are available for this feature:

BLAST of Lag0026590 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905529.1	9.3e-143	82.61	3,9-dihydroxypterocarpan 6A-monooxygenase [Benincasa hispida]	[more]
XP_023528514.1	2.9e-136	79.50	3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita pepo subsp. pepo]	[more]
KAA0043713.1	1.6e-134	79.19	3,9-dihydroxypterocarpan 6A-monooxygenase [Cucumis melo var. makuwa] >TYK25421.1...	[more]
XP_022934356.1	3.5e-134	78.88	3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita moschata]	[more]
KAG7017572.1	3.5e-134	78.88	3,9-dihydroxypterocarpan 6A-monooxygenase, partial [Cucurbita argyrosperma subsp...	[more]

BLAST of Lag0026590 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGT9	3.6e-49	38.44	Cytochrome P450 93B2 OS=Gerbera hybrida OX=18101 GN=CYP93B2 PE=1 SV=1	[more]
Q42798	4.8e-49	37.16	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1...	[more]
E9KBR8	1.8e-48	37.00	Cytochrome P450 93B16 OS=Glycine max OX=3847 GN=CYP93B16 PE=1 SV=1	[more]
Q9XHC6	9.9e-47	36.45	Beta-amyrin 24-hydroxylase OS=Glycine max OX=3847 GN=CYP93E1 PE=1 SV=1	[more]
O81973	1.4e-45	38.16	Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1	[more]

BLAST of Lag0026590 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DP65	7.7e-135	79.19	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A6J1F1K7	1.7e-134	78.88	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1J5T8	2.2e-134	78.70	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A1S3B805	6.5e-134	78.88	LOW QUALITY PROTEIN: 3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucumis melo O...	[more]
A0A0A0LBD2	6.3e-129	75.47	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G810500 PE=3 SV=1	[more]

BLAST of Lag0026590 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42250.1	8.0e-84	56.52	cytochrome P450, family 712, subfamily A, polypeptide 1	[more]
AT5G06905.1	8.3e-57	40.07	cytochrome P450, family 712, subfamily A, polypeptide 2	[more]
AT5G06900.1	9.2e-48	36.71	cytochrome P450, family 93, subfamily D, polypeptide 1	[more]
AT1G50520.1	1.5e-42	37.94	cytochrome P450, family 705, subfamily A, polypeptide 27	[more]
AT2G05180.1	7.5e-42	36.07	cytochrome P450, family 705, subfamily A, polypeptide 6	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 96..117 score: 26.13 coord: 190..208 score: 21.84 coord: 72..91 score: 31.07
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 48..313 e-value: 2.4E-29 score: 102.4
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 31..317 e-value: 1.3E-41 score: 144.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 47..315
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 10..314
None	No IPR available	PANTHER	PTHR24298:SF204	CYTOCHROME P450, FAMILY 712, SUBFAMILY A, POLYPEPTIDE 1	coord: 10..314

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0026590.1	Lag0026590.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen