Lag0026457 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0026457
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRetrotransposon protein
Locationchr10: 37164397 .. 37165257 (-)
RNA-Seq ExpressionLag0026457
SyntenyLag0026457
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATAGAAGATGTTTTGTCATACTATGTAGTTTGTTGAGAACGACTTCTGGGTTGGTAGGAACGAAAATCGTAGACGTGGAAGAGATGGTCATGATGTTCTTGCACATTGTTGCTCACGATGTTAAGAATCGAGTCATTAGAAGACAGTTTGCACGGTCAGGCGAGACCGTTTCTCGGCACTTCAACGTGACTTTGAGTGTCGTACTACGATTGTATGATGTTCTACTTAAGAAACCGGAACCGATCACGACTTCTTACCAAGATGGGAGGTGGAAATGGTTCAAGGTATGGATACTATATGTTAGTACACATCGTGAAGATCTTACAACGTCTTTCTGACATGTGTAATGCGATGTAGAAATAGAATTGTTTAAGTGCATTGGACGGTAAGTACATAAAAGTTCATGTTAGTTCGGCTGGTCAACCAAGGTATAGGACGCATAAGGGTGAGATTGCAACAAATGTATTGGGTGTTGTCTCGTCGAAAGGTGAATTAATTTTTGTTATGCCGAGATGGGAAGGTTTGGCTGTTGATTCTCGTGTACTCAGGGATGCAATATCACACCCCAATGGACTAATAGTGCCGAAGGGTATGGAACCCAATATCAATCTAAAATTGGAACACTTATGTCATCCTTAGGTATTAACCTTCTTTTCTGAACAGGCTACTCCTACCTTTGTGATGCTGGGTACCCAAACGTAGAGGGTTTCTTGGCACCTTATAGAGGAGAGCAGTACCACTTAACCGAGTGGCATGGGGGAGGCAATGCACCTATTACACCAAGAGAGTTCTTCAACATGAAGCATTCTTCTGCACGTAATGTTATCAAGAGAGCATTCGGTGCTCTGAAAGGATAG

mRNA sequence

ATGGATAGAAGATGTTTTGTCATACTATGTAGTTTGTTGAGAACGACTTCTGGGTTGGTAGGAACGAAAATCGTAGACGTGGAAGAGATGGTCATGATGTTCTTGCACATTGTTGCTCACGATGTTAAGAATCGAGTCATTAGAAGACAGTTTGCACGGTCAGGCGAGACCGTTTCTCGGCACTTCAACGTGACTTTGAGTGTCGTACTACGATTGTATGATGTTCTACTTAAGAAACCGGAACCGATCACGACTTCTTACCAAGATGGGAGGTGGAAATGGTTCAAGAATTGTTTAAGTGCATTGGACGGTAAGTACATAAAAGTTCATGTTAGTTCGGCTGGTCAACCAAGGTATAGGACGCATAAGGGTGAGATTGCAACAAATGTATTGGGTGTTGTCTCGTCGAAAGGTGAATTAATTTTTGTTATGCCGAGATGGGAAGGTTTGGCTGTTGATTCTCGTGTACTCAGGGATGCAATATCACACCCCAATGGACTAATAGTGCCGAAGGGCTACTCCTACCTTTGTGATGCTGGGTACCCAAACGTAGAGGGTTTCTTGGCACCTTATAGAGGAGAGCAGTACCACTTAACCGAGTGGCATGGGGGAGGCAATGCACCTATTACACCAAGAGAGTTCTTCAACATGAAGCATTCTTCTGCACGTAATGTTATCAAGAGAGCATTCGGTGCTCTGAAAGGATAG

Coding sequence (CDS)

ATGGATAGAAGATGTTTTGTCATACTATGTAGTTTGTTGAGAACGACTTCTGGGTTGGTAGGAACGAAAATCGTAGACGTGGAAGAGATGGTCATGATGTTCTTGCACATTGTTGCTCACGATGTTAAGAATCGAGTCATTAGAAGACAGTTTGCACGGTCAGGCGAGACCGTTTCTCGGCACTTCAACGTGACTTTGAGTGTCGTACTACGATTGTATGATGTTCTACTTAAGAAACCGGAACCGATCACGACTTCTTACCAAGATGGGAGGTGGAAATGGTTCAAGAATTGTTTAAGTGCATTGGACGGTAAGTACATAAAAGTTCATGTTAGTTCGGCTGGTCAACCAAGGTATAGGACGCATAAGGGTGAGATTGCAACAAATGTATTGGGTGTTGTCTCGTCGAAAGGTGAATTAATTTTTGTTATGCCGAGATGGGAAGGTTTGGCTGTTGATTCTCGTGTACTCAGGGATGCAATATCACACCCCAATGGACTAATAGTGCCGAAGGGCTACTCCTACCTTTGTGATGCTGGGTACCCAAACGTAGAGGGTTTCTTGGCACCTTATAGAGGAGAGCAGTACCACTTAACCGAGTGGCATGGGGGAGGCAATGCACCTATTACACCAAGAGAGTTCTTCAACATGAAGCATTCTTCTGCACGTAATGTTATCAAGAGAGCATTCGGTGCTCTGAAAGGATAG

Protein sequence

MDRRCFVILCSLLRTTSGLVGTKIVDVEEMVMMFLHIVAHDVKNRVIRRQFARSGETVSRHFNVTLSVVLRLYDVLLKKPEPITTSYQDGRWKWFKNCLSALDGKYIKVHVSSAGQPRYRTHKGEIATNVLGVVSSKGELIFVMPRWEGLAVDSRVLRDAISHPNGLIVPKGYSYLCDAGYPNVEGFLAPYRGEQYHLTEWHGGGNAPITPREFFNMKHSSARNVIKRAFGALKG
Homology
BLAST of Lag0026457 vs. NCBI nr
Match: XP_008455792.1 (PREDICTED: putative nuclease HARBI1 [Cucumis melo])

HSP 1 Score: 369.0 bits (946), Expect = 3.0e-98
Identity = 171/235 (72.77%), Postives = 196/235 (83.40%), Query Frame = 0

Query: 1   MDRRCFVILCSLLRTTSGLVGTKIVDVEEMVMMFLHIVAHDVKNRVIRRQFARSGETVSR 60
           MDRRCF ILC LLRTT+GLV T+++DVEEMV MFLHI+AHDVKNR+I+R+F RSGETVSR
Sbjct: 1   MDRRCFAILCHLLRTTAGLVETEVIDVEEMVAMFLHILAHDVKNRMIQREFVRSGETVSR 60

Query: 61  HFNVTLSVVLRLYDVLLKKPEPITTSYQDGRWKWFKNCLSALDGKYIKVHVSSAGQPRYR 120
           HFN+ L    RL+D LLKKP+P+T S  D RWKWF+NCL ALDG YIKV+VS+  +PRYR
Sbjct: 61  HFNIVLLAGFRLHDELLKKPQPVTNSCTDPRWKWFENCLGALDGTYIKVNVSATDRPRYR 120

Query: 121 THKGEIATNVLGVVSSKGELIFVMPRWEGLAVDSRVLRDAISHPNGLIVPKGYSYLCDAG 180
           T KGE+ATNVLG   +KG+ +FV+  WEG A DSR+LRDAIS  NGL VPKGY YLCDAG
Sbjct: 121 TRKGEVATNVLGACDTKGDFVFVLFGWEGSAADSRILRDAISRHNGLKVPKGYYYLCDAG 180

Query: 181 YPNVEGFLAPYRGEQYHLTEWHGGGNAPITPREFFNMKHSSARNVIKRAFGALKG 236
           YPN EGFLAPYRGE+YHL+EW G  NAP T REFFNMKHSSARNVI+RAFG LKG
Sbjct: 181 YPNAEGFLAPYRGERYHLSEWRGESNAPTTAREFFNMKHSSARNVIERAFGLLKG 235

BLAST of Lag0026457 vs. NCBI nr
Match: KAA0033290.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa] >TYK14818.1 putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 364.4 bits (934), Expect = 7.4e-97
Identity = 170/235 (72.34%), Postives = 196/235 (83.40%), Query Frame = 0

Query: 1   MDRRCFVILCSLLRTTSGLVGTKIVDVEEMVMMFLHIVAHDVKNRVIRRQFARSGETVSR 60
           MDRRCF ILC LLRTT+ LVGT+++DVEEMV MFLHI+AHD+KNR+I+R+F RSGETVSR
Sbjct: 1   MDRRCFAILCHLLRTTARLVGTEVIDVEEMVAMFLHILAHDMKNRIIQREFVRSGETVSR 60

Query: 61  HFNVTLSVVLRLYDVLLKKPEPITTSYQDGRWKWFKNCLSALDGKYIKVHVSSAGQPRYR 120
           HFN+ L  VLRL++ LLKKP+ +T S  D RWKWF+NCL ALD  YIKV+VS+  +PRY 
Sbjct: 61  HFNLVLLSVLRLHNELLKKPQLVTNSCMDPRWKWFENCLGALDDTYIKVNVSATDRPRYS 120

Query: 121 THKGEIATNVLGVVSSKGELIFVMPRWEGLAVDSRVLRDAISHPNGLIVPKGYSYLCDAG 180
           T KGE+A NVLGV  +KG+ +FV+  WEG A DSR+LRDAIS  NGL VPKGY YLCDAG
Sbjct: 121 TRKGEVAINVLGVCDTKGDFVFVLSGWEGSAADSRILRDAISRHNGLKVPKGYYYLCDAG 180

Query: 181 YPNVEGFLAPYRGEQYHLTEWHGGGNAPITPREFFNMKHSSARNVIKRAFGALKG 236
           Y NVEGFLAPYRGE+YHL+EWHGG NAP T REFFNMKHSSARNVIKRAF  LKG
Sbjct: 181 YSNVEGFLAPYRGERYHLSEWHGGSNAPTTAREFFNMKHSSARNVIKRAFWLLKG 235

BLAST of Lag0026457 vs. NCBI nr
Match: KAA0046727.1 (retrotransposon protein [Cucumis melo var. makuwa] >KAA0052100.1 retrotransposon protein [Cucumis melo var. makuwa] >KAA0056032.1 retrotransposon protein [Cucumis melo var. makuwa] >KAA0059561.1 retrotransposon protein [Cucumis melo var. makuwa] >KAA0065497.1 retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 353.2 bits (905), Expect = 1.7e-93
Identity = 162/235 (68.94%), Postives = 192/235 (81.70%), Query Frame = 0

Query: 1   MDRRCFVILCSLLRTTSGLVGTKIVDVEEMVMMFLHIVAHDVKNRVIRRQFARSGETVSR 60
           MDRRCF ILC LLRT +GL  T++VDVEEMV MFLHI+AHDVKNRVI+R+F RSGET+SR
Sbjct: 67  MDRRCFAILCHLLRTIAGLTSTEVVDVEEMVAMFLHILAHDVKNRVIQREFMRSGETISR 126

Query: 61  HFNVTLSVVLRLYDVLLKKPEPITTSYQDGRWKWFKNCLSALDGKYIKVHVSSAGQPRYR 120
           HFN+ L  V+RL+D LLKKP+P+     D RW+WF+NCL ALDG YIKV+V ++ + RYR
Sbjct: 127 HFNMVLLAVIRLHDELLKKPQPVPNECTDQRWRWFENCLGALDGTYIKVNVPASDRARYR 186

Query: 121 THKGEIATNVLGVVSSKGELIFVMPRWEGLAVDSRVLRDAISHPNGLIVPKGYSYLCDAG 180
           T KGE+ATNVLGV  +KG+ ++V+  WEG A DSR+LRDA+S PN L VPKGY YL DAG
Sbjct: 187 TRKGEVATNVLGVCDTKGDFVYVLAGWEGSAADSRILRDALSRPNRLKVPKGYYYLVDAG 246

Query: 181 YPNVEGFLAPYRGEQYHLTEWHGGGNAPITPREFFNMKHSSARNVIKRAFGALKG 236
           YPN EGFLAPYRG++YHL EW G  NAP T +EFFNMKHSSARNVI+RAFG LKG
Sbjct: 247 YPNAEGFLAPYRGQRYHLQEWRGPENAPSTSKEFFNMKHSSARNVIERAFGVLKG 301

BLAST of Lag0026457 vs. NCBI nr
Match: KAA0034843.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 353.2 bits (905), Expect = 1.7e-93
Identity = 162/235 (68.94%), Postives = 192/235 (81.70%), Query Frame = 0

Query: 1   MDRRCFVILCSLLRTTSGLVGTKIVDVEEMVMMFLHIVAHDVKNRVIRRQFARSGETVSR 60
           MDRRCF ILC LLRT +GL  T++VDVEEMV MFLHI+AHDVKNRVI+R+F RSGET+SR
Sbjct: 67  MDRRCFAILCHLLRTIAGLTSTEVVDVEEMVAMFLHILAHDVKNRVIQREFMRSGETISR 126

Query: 61  HFNVTLSVVLRLYDVLLKKPEPITTSYQDGRWKWFKNCLSALDGKYIKVHVSSAGQPRYR 120
           HFN+ L  V+RL+D LLKKP+P+     D RW+WF+NCL ALDG YIKV+V ++ + RYR
Sbjct: 127 HFNMVLLAVIRLHDELLKKPQPVPNECTDQRWRWFENCLGALDGTYIKVNVPASDRARYR 186

Query: 121 THKGEIATNVLGVVSSKGELIFVMPRWEGLAVDSRVLRDAISHPNGLIVPKGYSYLCDAG 180
           T KGE+ATNVLGV  +KG+ ++V+  WEG A DSR+LRDA+S PN L VPKGY YL DAG
Sbjct: 187 TRKGEVATNVLGVYDTKGDFVYVLTGWEGSAADSRILRDALSRPNRLKVPKGYYYLVDAG 246

Query: 181 YPNVEGFLAPYRGEQYHLTEWHGGGNAPITPREFFNMKHSSARNVIKRAFGALKG 236
           YPN EGFLAPYRG++YHL EW G  NAP T +EFFNMKHSSARNVI+RAFG LKG
Sbjct: 247 YPNAEGFLAPYRGQRYHLQEWRGPKNAPSTSKEFFNMKHSSARNVIERAFGVLKG 301

BLAST of Lag0026457 vs. NCBI nr
Match: KAA0048361.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 352.1 bits (902), Expect = 3.8e-93
Identity = 162/235 (68.94%), Postives = 192/235 (81.70%), Query Frame = 0

Query: 1   MDRRCFVILCSLLRTTSGLVGTKIVDVEEMVMMFLHIVAHDVKNRVIRRQFARSGETVSR 60
           MDRRCF ILC LLRT +GL  T++VDVEEMV MFLHI+AHDVKNRVI+R+F RSGET+SR
Sbjct: 67  MDRRCFAILCHLLRTIAGLTSTEVVDVEEMVAMFLHILAHDVKNRVIQREFMRSGETISR 126

Query: 61  HFNVTLSVVLRLYDVLLKKPEPITTSYQDGRWKWFKNCLSALDGKYIKVHVSSAGQPRYR 120
           HFN+ L  V+RL++ LLKKP+P+     D RWKWF+NCL ALDG YIKV+V ++ + RYR
Sbjct: 127 HFNMVLLAVIRLHEELLKKPQPVPNECTDQRWKWFENCLGALDGTYIKVNVPASDRARYR 186

Query: 121 THKGEIATNVLGVVSSKGELIFVMPRWEGLAVDSRVLRDAISHPNGLIVPKGYSYLCDAG 180
           T KGE+ATNVLGV  +KG+ ++V+  WEGLA DSR+LRDA+S PN L VPKGY YL DAG
Sbjct: 187 TRKGEVATNVLGVCDTKGDFVYVLAGWEGLAADSRILRDALSRPNRLKVPKGYYYLVDAG 246

Query: 181 YPNVEGFLAPYRGEQYHLTEWHGGGNAPITPREFFNMKHSSARNVIKRAFGALKG 236
           Y N EGFLAPYRG++YHL EW G  NAP T +EFFNMKHSSARNVI+RAFG LKG
Sbjct: 247 YQNAEGFLAPYRGQRYHLQEWRGPENAPSTSKEFFNMKHSSARNVIERAFGVLKG 301

BLAST of Lag0026457 vs. ExPASy TrEMBL
Match: A0A1S3C1U8 (putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103495887 PE=3 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.5e-98
Identity = 171/235 (72.77%), Postives = 196/235 (83.40%), Query Frame = 0

Query: 1   MDRRCFVILCSLLRTTSGLVGTKIVDVEEMVMMFLHIVAHDVKNRVIRRQFARSGETVSR 60
           MDRRCF ILC LLRTT+GLV T+++DVEEMV MFLHI+AHDVKNR+I+R+F RSGETVSR
Sbjct: 1   MDRRCFAILCHLLRTTAGLVETEVIDVEEMVAMFLHILAHDVKNRMIQREFVRSGETVSR 60

Query: 61  HFNVTLSVVLRLYDVLLKKPEPITTSYQDGRWKWFKNCLSALDGKYIKVHVSSAGQPRYR 120
           HFN+ L    RL+D LLKKP+P+T S  D RWKWF+NCL ALDG YIKV+VS+  +PRYR
Sbjct: 61  HFNIVLLAGFRLHDELLKKPQPVTNSCTDPRWKWFENCLGALDGTYIKVNVSATDRPRYR 120

Query: 121 THKGEIATNVLGVVSSKGELIFVMPRWEGLAVDSRVLRDAISHPNGLIVPKGYSYLCDAG 180
           T KGE+ATNVLG   +KG+ +FV+  WEG A DSR+LRDAIS  NGL VPKGY YLCDAG
Sbjct: 121 TRKGEVATNVLGACDTKGDFVFVLFGWEGSAADSRILRDAISRHNGLKVPKGYYYLCDAG 180

Query: 181 YPNVEGFLAPYRGEQYHLTEWHGGGNAPITPREFFNMKHSSARNVIKRAFGALKG 236
           YPN EGFLAPYRGE+YHL+EW G  NAP T REFFNMKHSSARNVI+RAFG LKG
Sbjct: 181 YPNAEGFLAPYRGERYHLSEWRGESNAPTTAREFFNMKHSSARNVIERAFGLLKG 235

BLAST of Lag0026457 vs. ExPASy TrEMBL
Match: A0A5A7SQU2 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G00070 PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 3.6e-97
Identity = 170/235 (72.34%), Postives = 196/235 (83.40%), Query Frame = 0

Query: 1   MDRRCFVILCSLLRTTSGLVGTKIVDVEEMVMMFLHIVAHDVKNRVIRRQFARSGETVSR 60
           MDRRCF ILC LLRTT+ LVGT+++DVEEMV MFLHI+AHD+KNR+I+R+F RSGETVSR
Sbjct: 1   MDRRCFAILCHLLRTTARLVGTEVIDVEEMVAMFLHILAHDMKNRIIQREFVRSGETVSR 60

Query: 61  HFNVTLSVVLRLYDVLLKKPEPITTSYQDGRWKWFKNCLSALDGKYIKVHVSSAGQPRYR 120
           HFN+ L  VLRL++ LLKKP+ +T S  D RWKWF+NCL ALD  YIKV+VS+  +PRY 
Sbjct: 61  HFNLVLLSVLRLHNELLKKPQLVTNSCMDPRWKWFENCLGALDDTYIKVNVSATDRPRYS 120

Query: 121 THKGEIATNVLGVVSSKGELIFVMPRWEGLAVDSRVLRDAISHPNGLIVPKGYSYLCDAG 180
           T KGE+A NVLGV  +KG+ +FV+  WEG A DSR+LRDAIS  NGL VPKGY YLCDAG
Sbjct: 121 TRKGEVAINVLGVCDTKGDFVFVLSGWEGSAADSRILRDAISRHNGLKVPKGYYYLCDAG 180

Query: 181 YPNVEGFLAPYRGEQYHLTEWHGGGNAPITPREFFNMKHSSARNVIKRAFGALKG 236
           Y NVEGFLAPYRGE+YHL+EWHGG NAP T REFFNMKHSSARNVIKRAF  LKG
Sbjct: 181 YSNVEGFLAPYRGERYHLSEWHGGSNAPTTAREFFNMKHSSARNVIKRAFWLLKG 235

BLAST of Lag0026457 vs. ExPASy TrEMBL
Match: A0A5A7TXW1 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold298G00370 PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 8.3e-94
Identity = 162/235 (68.94%), Postives = 192/235 (81.70%), Query Frame = 0

Query: 1   MDRRCFVILCSLLRTTSGLVGTKIVDVEEMVMMFLHIVAHDVKNRVIRRQFARSGETVSR 60
           MDRRCF ILC LLRT +GL  T++VDVEEMV MFLHI+AHDVKNRVI+R+F RSGET+SR
Sbjct: 67  MDRRCFAILCHLLRTIAGLTSTEVVDVEEMVAMFLHILAHDVKNRVIQREFMRSGETISR 126

Query: 61  HFNVTLSVVLRLYDVLLKKPEPITTSYQDGRWKWFKNCLSALDGKYIKVHVSSAGQPRYR 120
           HFN+ L  V+RL+D LLKKP+P+     D RW+WF+NCL ALDG YIKV+V ++ + RYR
Sbjct: 127 HFNMVLLAVIRLHDELLKKPQPVPNECTDQRWRWFENCLGALDGTYIKVNVPASDRARYR 186

Query: 121 THKGEIATNVLGVVSSKGELIFVMPRWEGLAVDSRVLRDAISHPNGLIVPKGYSYLCDAG 180
           T KGE+ATNVLGV  +KG+ ++V+  WEG A DSR+LRDA+S PN L VPKGY YL DAG
Sbjct: 187 TRKGEVATNVLGVCDTKGDFVYVLAGWEGSAADSRILRDALSRPNRLKVPKGYYYLVDAG 246

Query: 181 YPNVEGFLAPYRGEQYHLTEWHGGGNAPITPREFFNMKHSSARNVIKRAFGALKG 236
           YPN EGFLAPYRG++YHL EW G  NAP T +EFFNMKHSSARNVI+RAFG LKG
Sbjct: 247 YPNAEGFLAPYRGQRYHLQEWRGPENAPSTSKEFFNMKHSSARNVIERAFGVLKG 301

BLAST of Lag0026457 vs. ExPASy TrEMBL
Match: A0A5A7SWD8 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold515G00010 PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 8.3e-94
Identity = 162/235 (68.94%), Postives = 192/235 (81.70%), Query Frame = 0

Query: 1   MDRRCFVILCSLLRTTSGLVGTKIVDVEEMVMMFLHIVAHDVKNRVIRRQFARSGETVSR 60
           MDRRCF ILC LLRT +GL  T++VDVEEMV MFLHI+AHDVKNRVI+R+F RSGET+SR
Sbjct: 67  MDRRCFAILCHLLRTIAGLTSTEVVDVEEMVAMFLHILAHDVKNRVIQREFMRSGETISR 126

Query: 61  HFNVTLSVVLRLYDVLLKKPEPITTSYQDGRWKWFKNCLSALDGKYIKVHVSSAGQPRYR 120
           HFN+ L  V+RL+D LLKKP+P+     D RW+WF+NCL ALDG YIKV+V ++ + RYR
Sbjct: 127 HFNMVLLAVIRLHDELLKKPQPVPNECTDQRWRWFENCLGALDGTYIKVNVPASDRARYR 186

Query: 121 THKGEIATNVLGVVSSKGELIFVMPRWEGLAVDSRVLRDAISHPNGLIVPKGYSYLCDAG 180
           T KGE+ATNVLGV  +KG+ ++V+  WEG A DSR+LRDA+S PN L VPKGY YL DAG
Sbjct: 187 TRKGEVATNVLGVYDTKGDFVYVLTGWEGSAADSRILRDALSRPNRLKVPKGYYYLVDAG 246

Query: 181 YPNVEGFLAPYRGEQYHLTEWHGGGNAPITPREFFNMKHSSARNVIKRAFGALKG 236
           YPN EGFLAPYRG++YHL EW G  NAP T +EFFNMKHSSARNVI+RAFG LKG
Sbjct: 247 YPNAEGFLAPYRGQRYHLQEWRGPKNAPSTSKEFFNMKHSSARNVIERAFGVLKG 301

BLAST of Lag0026457 vs. ExPASy TrEMBL
Match: A0A5A7U476 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G00800 PE=3 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.9e-93
Identity = 162/235 (68.94%), Postives = 192/235 (81.70%), Query Frame = 0

Query: 1   MDRRCFVILCSLLRTTSGLVGTKIVDVEEMVMMFLHIVAHDVKNRVIRRQFARSGETVSR 60
           MDRRCF ILC LLRT +GL  T++VDVEEMV MFLHI+AHDVKNRVI+R+F RSGET+SR
Sbjct: 67  MDRRCFAILCHLLRTIAGLTSTEVVDVEEMVAMFLHILAHDVKNRVIQREFMRSGETISR 126

Query: 61  HFNVTLSVVLRLYDVLLKKPEPITTSYQDGRWKWFKNCLSALDGKYIKVHVSSAGQPRYR 120
           HFN+ L  V+RL++ LLKKP+P+     D RWKWF+NCL ALDG YIKV+V ++ + RYR
Sbjct: 127 HFNMVLLAVIRLHEELLKKPQPVPNECTDQRWKWFENCLGALDGTYIKVNVPASDRARYR 186

Query: 121 THKGEIATNVLGVVSSKGELIFVMPRWEGLAVDSRVLRDAISHPNGLIVPKGYSYLCDAG 180
           T KGE+ATNVLGV  +KG+ ++V+  WEGLA DSR+LRDA+S PN L VPKGY YL DAG
Sbjct: 187 TRKGEVATNVLGVCDTKGDFVYVLAGWEGLAADSRILRDALSRPNRLKVPKGYYYLVDAG 246

Query: 181 YPNVEGFLAPYRGEQYHLTEWHGGGNAPITPREFFNMKHSSARNVIKRAFGALKG 236
           Y N EGFLAPYRG++YHL EW G  NAP T +EFFNMKHSSARNVI+RAFG LKG
Sbjct: 247 YQNAEGFLAPYRGQRYHLQEWRGPENAPSTSKEFFNMKHSSARNVIERAFGVLKG 301

BLAST of Lag0026457 vs. TAIR 10
Match: AT5G41980.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G43722.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 141.4 bits (355), Expect = 9.6e-34
Identity = 81/234 (34.62%), Postives = 117/234 (50.00%), Query Frame = 0

Query: 1   MDRRCFVILCSLLRTTSGLVGTKIVDVEEMVMMFLHIVAHDVKNRVIRRQFARSGETVSR 60
           MD+  F  LC LL+T   L  T  + +E  + +FL I+ H+++ R ++  F  SGET+SR
Sbjct: 48  MDKPVFYKLCDLLQTRGLLRHTNRIKIEAQLAIFLFIIGHNLRTRAVQELFCYSGETISR 107

Query: 61  HFNVTLSVVLRLYDVLLKKPEPITTSYQDGRWKWFKNCLSALDGKYIKVHVSSAGQPRYR 120
           HFN  L+ V+ +     +      T   D    +FK+C+  +D  +I V V    Q  +R
Sbjct: 108 HFNNVLNAVIAISKDFFQPNSNSDTLENDD--PYFKDCVGVVDSFHIPVMVGVDEQGPFR 167

Query: 121 THKGEIATNVLGVVSSKGELIFVMPRWEGLAVDSRVLRDAISHPNGLIVPKGYSYLCDAG 180
              G +  NVL   S      +V+  WEG A D +VL  A++  N L VP+G  Y+ D  
Sbjct: 168 NGNGLLTQNVLAASSFDLRFNYVLAGWEGSASDQQVLNAALTRRNKLQVPQGKYYIVDNK 227

Query: 181 YPNVEGFLAPYRGEQYHLTEWHGGGNAPITPREFFNMKHSSARNVIKRAFGALK 235
           YPN+ GF+APY G   +  E           +E FN +H      I R FGALK
Sbjct: 228 YPNLPGFIAPYHGVSTNSRE---------EAKEMFNERHKLLHRAIHRTFGALK 270

BLAST of Lag0026457 vs. TAIR 10
Match: AT1G43722.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G28730.1); Has 924 Blast hits to 912 proteins in 109 species: Archae - 0; Bacteria - 0; Metazoa - 222; Fungi - 31; Plants - 661; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink). )

HSP 1 Score: 113.6 bits (283), Expect = 2.1e-25
Identity = 73/243 (30.04%), Postives = 111/243 (45.68%), Query Frame = 0

Query: 1   MDRRCFVILCSLLRTTSGLVGTKIVDVEEMVMMFLHIVAHDVKNRVIRRQFARSGETVSR 60
           M   CF  LC++L+T   L  T  + +EE V MFL I  H+   R +  +F R+ ETV R
Sbjct: 72  MSLPCFTTLCNMLQTNYDLQPTLNISIEESVAMFLRICGHNEVYRDVGLRFGRNQETVQR 131

Query: 61  HFNVTLSVVLRLYDVLLKKPE-------PITTSYQDGRWKWFKNCLSALDGKYIKVHVSS 120
            F   L+    L    ++ P        P         W +F   + A+DG ++ V V  
Sbjct: 132 KFREVLTATELLACDYIRTPTRQELYRIPERLQVDQRYWPYFSGFVGAMDGTHVCVKVKP 191

Query: 121 AGQPRYRTHKGEIATNVLGVVSSKGELIFVMPRWEGLAVDSRVLRDAISHPNGLIVPKGY 180
             Q  Y       + N++ +   K    ++     G   D+ VL+ A    +   +P   
Sbjct: 192 DLQGMYWNRHDNASLNIMAICDLKMLFTYIWNGAPGSCYDTAVLQIAQQSDSEFPLPPSE 251

Query: 181 S-YLCDAGYPNVEGFLAPYRGE-----QYHLTEWHGGGNAPITPREFFNMKHSSARNVIK 231
             YL D+GYPN +G LAPYR       +YH+++++ G   P    E FN  H+S R+VI+
Sbjct: 252 KYYLVDSGYPNKQGLLAPYRSSRNRVVRYHMSQFYYGPR-PRNKHELFNQCHTSLRSVIE 311

BLAST of Lag0026457 vs. TAIR 10
Match: AT5G35695.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G41980.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 87.0 bits (214), Expect = 2.1e-17
Identity = 45/94 (47.87%), Postives = 55/94 (58.51%), Query Frame = 0

Query: 141 IFVMPRWEGLAVDSRVLRDAISHPNGLIVPKGYSYLCDAGYPNVEGFLAPYRGEQYHLTE 200
           I+V+  WEG A DSRVL DA+             YL D G+ N   FLAP+RG +YHL E
Sbjct: 26  IYVLSGWEGSAHDSRVLSDALRK----------FYLVDCGFANRLNFLAPFRGVRYHLQE 85

Query: 201 WHGGGNAPITPREFFNMKHSSARNVIKRAFGALK 235
           + G    P TP E FN++H S RNVI+R FG  K
Sbjct: 86  FAGQRRDPETPHELFNLRHVSLRNVIERIFGIFK 109

BLAST of Lag0026457 vs. TAIR 10
Match: AT5G28950.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G41980.1); Has 448 Blast hits to 446 proteins in 74 species: Archae - 0; Bacteria - 0; Metazoa - 31; Fungi - 21; Plants - 396; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 65.5 bits (158), Expect = 6.7e-11
Identity = 31/81 (38.27%), Postives = 50/81 (61.73%), Query Frame = 0

Query: 92  WKWFKNCLSALDGKYIKVHVSSAGQPRYRTHKGEIATNVLGVVSSKGELIFVMPRWEGLA 151
           + +FK+C+ A+D  +I   VS    P +R  KG+I+ N+L   +   E ++V+  WEG A
Sbjct: 19  YPYFKDCVGAIDDTHIFAMVSQKKMPSFRNRKGDISQNMLAACNFDVEFMYVLSGWEGSA 78

Query: 152 VDSRVLRDAIS-HPNGLIVPK 172
            DS+VL DA++ + N L VP+
Sbjct: 79  HDSKVLNDALTRNSNRLPVPE 99

BLAST of Lag0026457 vs. TAIR 10
Match: AT5G28730.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G43722.1); Has 496 Blast hits to 496 proteins in 68 species: Archae - 0; Bacteria - 0; Metazoa - 3; Fungi - 23; Plants - 470; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 62.0 bits (149), Expect = 7.4e-10
Identity = 60/231 (25.97%), Postives = 86/231 (37.23%), Query Frame = 0

Query: 1   MDRRCFVILCSLLRTTSGLVGTKIVDVEEMVMMFLHIVAHDVKNRVIRRQFARSGETVSR 60
           M    F  LC +L    GL  +  + ++E V +FL I A +   R I  +F  + ET+ R
Sbjct: 30  MSSEAFTQLCEILHGKYGLQSSTNISLDESVAIFLIICASNDTQRDIALRFGHAQETIWR 89

Query: 61  HFNVTLSVVLRLYDVLLK-----KPEPITTSYQDGRWKWFKNCLSALDGKYIKVHVSSAG 120
            F+  L  + RL    ++     +   I+   QD    W                     
Sbjct: 90  KFHDVLKAMERLAVEYIRPRKVEELRAISNRLQDDTRYW--------------------- 149

Query: 121 QPRYRTHKGEIATNVLGVVSSKGELIFVMPRWEGLAVDSRVLRDAIS-HPNGLIVPKGYS 180
            P      G  + NVL +        +      G   D+RVL  AIS  P   + P    
Sbjct: 150 -PFLMDLLGIASFNVLAICDLDMLFTYCFVGMAGSTHDARVLSAAISDDPLFHVPPDSKY 209

Query: 181 YLCDAGYPNVEGFLAPYRGEQYHLTEWHGGGNAPITPREFFNMKHSSARNV 226
           YL D+GY N  G+LAPYR E     +        +   E  N+K     NV
Sbjct: 210 YLVDSGYANKRGYLAPYRREHREAQDIISNNFLTVNLFETHNIKDYDFDNV 238

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008455792.13.0e-9872.77PREDICTED: putative nuclease HARBI1 [Cucumis melo][more]
KAA0033290.17.4e-9772.34putative nuclease HARBI1 [Cucumis melo var. makuwa] >TYK14818.1 putative nucleas... [more]
KAA0046727.11.7e-9368.94retrotransposon protein [Cucumis melo var. makuwa] >KAA0052100.1 retrotransposon... [more]
KAA0034843.11.7e-9368.94retrotransposon protein [Cucumis melo var. makuwa][more]
KAA0048361.13.8e-9368.94retrotransposon protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A1S3C1U81.5e-9872.77putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103495887 PE=3 SV=1[more]
A0A5A7SQU23.6e-9772.34Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A5A7TXW18.3e-9468.94Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5A7SWD88.3e-9468.94Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A5A7U4761.9e-9368.94Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
Match NameE-valueIdentityDescription
AT5G41980.19.6e-3434.62CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int... [more]
AT1G43722.12.1e-2530.04unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G35695.12.1e-1747.87CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int... [more]
AT5G28950.16.7e-1138.27unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G28730.17.4e-1025.97unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027806Harbinger transposase-derived nuclease domainPFAMPF13359DDE_Tnp_4coord: 102..235
e-value: 1.1E-11
score: 44.7
NoneNo IPR availablePANTHERPTHR22930:SF201NUCLEASE HARBI1-LIKE PROTEINcoord: 1..234
NoneNo IPR availablePANTHERPTHR22930UNCHARACTERIZEDcoord: 1..234

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0026457.1Lag0026457.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding