Lag0026428 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0026428
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCytoplasmic tRNA 2-thiolation protein 2
Locationchr10: 36659598 .. 36663268 (+)
RNA-Seq ExpressionLag0026428
SyntenyLag0026428
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTGTAATTCCTCAGCCTGCCAGTCGAACTGCTACAGAAATCAAGATGACGAAGAAACAGTCGAACAAGATTCACCTCCAACAACCAAGCCCGATACAGCAGCCGCCGTGGAGTCTCGTCGAGTTGATACGAACGACGACAGTAGTAATACGCATCTCTGCTTGAAGTGCAAGTCCAAGGAGCGTATGTCTAGCTCTGGAGTAGGAGACGATGGCCGCTTTTGCAGCGATTGCTTTCGTAGCAACTTGTTTGCTAAGTTCCGGCAGGCTGTAAACTCGAACGCCATGATCTCTCCTTCGGATAAAGTTCTTGTTGCCTTCTCTGGGGTCCTTGTTCCAGGTTCTTCCTTTTTCTTTTGAGACACTGTATATTTTCTTTTTGTAAAGAATTGGTTCATGGTTGTAGGTGTTCAGAGAACTGTTTTTTCTTTTTGTTTTTTGGATGTTGCGAGTGGACATGATAGGTTTTCTGGCTGAATTTTAGGTTAGAATCGAGAGTGCTAGTAATTGTTTCATTTTTTTTTTTTTTTCTGTTAATTGTGCGCCTCATTCCAACGGTTCGCATATATTTTCTGTTAGCATTTGCGAAGAGCACTGTTCCACCTCCACAAGCGAGAATATCTTAAGGAGAAAGGAGAAGGGAGAAGATCAGAGAATACTTAGGAATAACTGAGCATGAGCATTAGTGTGACTCTGTAGTGGTTAAGCCATGGACTAGAGCCAATGCCCAGCAAAGCCAAGTAAGGAGAGAACTATGTCAATCGTGATCCTAAGCTCTCAGTCTCTTTACTTATAGTTATAGGAGGAGAGTAATCATTACCCCATTGTCGTTGTTGCCATGTCTAACTTCTCAGTAATAGTGGCCTAACTTTAGAAATGTGTGTCATGCATTCACTAGCTAAATGCAAACAATTCCTCATCTTCTTCCTTAGTTTTGGGTGATCAATTCAAGCACTTAGCCGAGCTACTATTTCAAGTAGGTTTTATTGACCTTTCTACTAGGGTATGTAAGTTGCACTTGTCTACCTTGATAGGTATAGTGTGCTCATCCTATCTTATTCTATTGGACAAGTCTTAATGATCTTGATCGGTTTATGTTGCCTTCCTAATTTGTGCCAAAGCCACGTAAGCATATTATTGGAGAATTTCACTCATCGCACTTAAAAACATAAAAAAACAAAATATAAAACACTTTTTATTGATATAGCAAAACGTTATCAGAAGTTATGCTATAACAATTTGACCGAAAATAAAAGAAGTTTGCTCCTAATGGAGTTTACAAAAACTCACCCAAGGAGCATAGTTACTAAAGGCATTATCTATACTATAGAGGTCGTGTAGGACAAACGCACAACTTAGCATACTTGAAAACTTCGTCTTAATCTATTGACTTATCCAATAAAGAGCTTAGAGGGAATGAGTTCAATCCATGGTGGCCATCTACCTAGGATTTAATATCCTACGAGTTTCCTTGACAACCAAATGTAGTAGGGTCAGGCGGTTGTCCTGTGAGATTAGTCAAGGTGCGCGTAAGCTGGCTCGGACATTCACGGATATTAAAAAAAAACCTATTGACTTATGGTTGAACACTGTTGATTTTTTTCACGGCTACTATAACTTCTACTTGGAAACAAAGTACCATAAATTTAAAGGTAAGCCCTATAATTTGATTCAACCCCAATAACTTGAAACACTGGCTCGTTCATGATTCTCCAAATTGCACCTTTTGAGTCAGTAAAAAGTCCCTTACTGCCACCCACTCAACATTCCCGAACAAATCCTCTTCAATACATTCATTAGAACATTTTCATGAAGGCCATGTCACGCTTGGAAGGGATTAAGCCGTCTCCCAAATGTCAGGCCACCCATCATGAACATAGAGTCTAATCTTTCAACCAACCTTTTCGGGAAAGAGGCAAATCTTAGATCCCCTTCTTCTAGCTAATAGTTATTTGATGAGTGTAGGAGAAGGAAGAAAAGAGGTTTTTTAATGAAACTTTGACCTAGAGAAGAGCATGGTGTTAAGTGAAAAAGGCCTCTATTTCCTGATGAAGGTGGAAATTGGGATAAGTAATTTTTCTGGTGCCAATATCCCTTTTTTGGTTATTGACAAACCCAGGGGGAGAAGAGAATTATTGCTTCTTGTAGTTTGAGGCAAGGAATTTCCTCTTTTGTGTTGGTGGGTAGATTTCTTTATTAGAATCCTTTCTTTGGTTTCTGATTCCAACTTTACTGAGGATTTCTAATTAGTAGAATTCATCTCAGTATCAACCACCTCCTACTCATGAATGACATTCTCTCTCTTATTTTTAGAGAAACAATTTCGTTAGTGAGATGAAACTGCAAAAGGGGAGGAAAGCCAAACCCCATCCAAAGGAAGTTACAACATTTTCATCATTGAAAAAAACAATGCAGATTGGTCCGTAACATTTGCAGTTTGTTCAGAGTTATTAGGATCTTTGAGCAGGCCTTTAGGTTTATTATAGTTAATCTGAGTAAACCAATGTTGTTCAGCCTAAACATGATGATAGGAATGTACCTAAGGTGTAATTAGGAATATTAGTAAGGGTATATTAGCAGTTAGTTAGAGAGTTGTTAGAGTATGGAGTTATAAATAGTGGGAGTGGGAAAGGGGAAGATAGAGAATTAAGCAGTGAGTAGTTACTCTTGAGTGTTCTCAAGGAGGGAAGGTCCAAGTACCTCGAACACTTGGGTTATCGTAATAATACATTGTGGTTCTATTCCGGTTCTTATCACGTGAGCACTAATGATACTTATTTTGTGGCTCAAAATTTCTTTTGTCAGGTTGGTGATTAGCCCATTATCTATCTCAGCATGCCCATCGATGGTAATTCCAATAACAAAGCTTTCTAGGTGCCAATGGGACCACTTTTCATTAATCAACTCAAAATCTTAGTTTCGATACGCTGCAAACTAAAGGACACTGCTTGTGGATTTATTATTAAAACTCTTGAACTAACAAAATAACCCCCTAGAATATTCTCTTGGCCAAATATAATTCTCTCGACAAGAACCTTGAGGTTTTAGCTTCCACAAGTGTTTTAAAAGGCTCAAGGCACACTAAAGCGCAATGACCTCCTGGGGCCTAGGTGCAAGGCGCACAAAAAGGCGCGGGCTTTTTTCTCTAAGGCGCACTATATAGAAAAAAAACTTACAAAATATATACATGTACATAAAACACTTTCATGGCAAGTAATGAAGTTTTTAACCAAGAAAAATAGATAAAAGATAAATAAGGTTCAAATATTAGTCTTCCAACATAGAGTCAAATGTCAATACTCCACAAAAAAATAGTGAGTTTAGTATGGTTAATATGCCTTCTGACACTCCAAACGATCAAGTATGATGATGTCAATACTCCACAAAAAATATATGCCTTGAATATGATATTTGACACTTCGAACGACAAGTACGGGGTTCGGGGCAACGCCCCAGAAGCTAAATTTAAAATTAGCTCCATAACAAGTATTTTGGCTTAAAAAAATTTAAAACATGTATAAAAATGTGAAGCCCCAGAGGCGCCTAAGGCGCGCGCCTCGGGGGCTTCGAGGAAGGCGCGCGCCTTGGCCGCTCCTTCCTCACTGCGCCTCGCTTCTTCGAAGCGTGGCGCTAACGTTGCGCCTTGCGCGGCTGGGCGTGCCTTTTAA

mRNA sequence

ATGGCGTGTAATTCCTCAGCCTGCCAGTCGAACTGCTACAGAAATCAAGATGACGAAGAAACAGTCGAACAAGATTCACCTCCAACAACCAAGCCCGATACAGCAGCCGCCGTGGAGTCTCGTCGAGTTGATACGAACGACGACAGTAGTAATACGCATCTCTGCTTGAAGTGCAAGTCCAAGGAGCGTATGTCTAGCTCTGGAGTAGGAGACGATGGCCGCTTTTGCAGCGATTGCTTTCGTAGCAACTTGTTTGCTAAGTTCCGGCAGGCTGTAAACTCGAACGCCATGATCTCTCCTTCGGATAAAGTTCTTGTTGCCTTCTCTGGGGTCCTTGTTCCAGTGGTTAAGCCATGGACTAGAGCCAATGCCCAGCAAAGCCAACTAAATGCAAACAATTCCTCATCTTCTTCCTTAGTTTTGGGTGATCAATTCAAGCACTTAGCCGAGCTACTATTTCAAGTAGGTTTTATTGACCTTTCTACTAGGGTATCCCCAGAGGCGCCTAAGGCGCGCGCCTCGGGGGCTTCGAGGAAGGCGCGCGCCTTGGCCGCTCCTTCCTCACTGCGCCTCGCTTCTTCGAAGCGTGGCGCTAACGTTGCGCCTTGCGCGGCTGGGCGTGCCTTTTAA

Coding sequence (CDS)

ATGGCGTGTAATTCCTCAGCCTGCCAGTCGAACTGCTACAGAAATCAAGATGACGAAGAAACAGTCGAACAAGATTCACCTCCAACAACCAAGCCCGATACAGCAGCCGCCGTGGAGTCTCGTCGAGTTGATACGAACGACGACAGTAGTAATACGCATCTCTGCTTGAAGTGCAAGTCCAAGGAGCGTATGTCTAGCTCTGGAGTAGGAGACGATGGCCGCTTTTGCAGCGATTGCTTTCGTAGCAACTTGTTTGCTAAGTTCCGGCAGGCTGTAAACTCGAACGCCATGATCTCTCCTTCGGATAAAGTTCTTGTTGCCTTCTCTGGGGTCCTTGTTCCAGTGGTTAAGCCATGGACTAGAGCCAATGCCCAGCAAAGCCAACTAAATGCAAACAATTCCTCATCTTCTTCCTTAGTTTTGGGTGATCAATTCAAGCACTTAGCCGAGCTACTATTTCAAGTAGGTTTTATTGACCTTTCTACTAGGGTATCCCCAGAGGCGCCTAAGGCGCGCGCCTCGGGGGCTTCGAGGAAGGCGCGCGCCTTGGCCGCTCCTTCCTCACTGCGCCTCGCTTCTTCGAAGCGTGGCGCTAACGTTGCGCCTTGCGCGGCTGGGCGTGCCTTTTAA

Protein sequence

MACNSSACQSNCYRNQDDEETVEQDSPPTTKPDTAAAVESRRVDTNDDSSNTHLCLKCKSKERMSSSGVGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGVLVPVVKPWTRANAQQSQLNANNSSSSSLVLGDQFKHLAELLFQVGFIDLSTRVSPEAPKARASGASRKARALAAPSSLRLASSKRGANVAPCAAGRAF
Homology
[Close]
BLAST of Lag0026428 vs. NCBI nr
Match: XP_004136614.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucumis sativus] >XP_011652154.1 cytoplasmic tRNA 2-thiolation protein 2 [Cucumis sativus] >XP_031737791.1 cytoplasmic tRNA 2-thiolation protein 2 [Cucumis sativus] >KGN59393.1 hypothetical protein Csa_001562 [Cucumis sativus])

HSP 1 Score: 193.0 bits (489), Expect = 2.6e-45
Identity = 101/141 (71.63%), Postives = 116/141 (82.27%), Query Frame = 0 

Query: 1   MACNSSACQSNCYRNQDDEETVEQDSPPTTKPDTAAAVESRRVDTNDDSSNTHLCLKCKS 60
           MACNSSACQSNCYRNQ DE T+EQDSPP+++PDT AA+E+RRV T DD+SNT+LC KCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60

Query: 61  KERMSSSGVGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGVLVPVVKPWT 120
           KERMS+SGVGDDGRFCSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSG       P +
Sbjct: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSG------GPCS 120

Query: 121 RANAQ---QSQLNANNSSSSS 139
           R   Q   + Q+ A+N+  +S
Sbjct: 121 RVALQFVHEMQVKAHNNFEAS 135

[Close]
BLAST of Lag0026428 vs. NCBI nr
Match: XP_038905927.1 (cytoplasmic tRNA 2-thiolation protein 2 [Benincasa hispida])

HSP 1 Score: 192.2 bits (487), Expect = 4.5e-45
Identity = 95/110 (86.36%), Postives = 102/110 (92.73%), Query Frame = 0 

Query: 1   MACNSSACQSNCYRNQDDEETVEQDSPPTTKPDTAAAVESRRVDTNDDSSNTHLCLKCKS 60
           MACNSSACQSNCYRNQ DE T+EQ SPP+++ DTAAAVES +VDTNDDSS+THLC KCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQVSPPSSRSDTAAAVESCQVDTNDDSSHTHLCFKCKS 60

Query: 61  KERMSSSGVGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSG 111
           KER+SSSGVGDDGR CSDCFRSNLF KFRQAVNSNAMISPSDKVLVAFSG
Sbjct: 61  KERISSSGVGDDGRLCSDCFRSNLFGKFRQAVNSNAMISPSDKVLVAFSG 110

[Close]
BLAST of Lag0026428 vs. NCBI nr
Match: XP_008443191.1 (PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo] >XP_008443192.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo] >KAA0053926.1 cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo var. makuwa] >TYK25478.1 cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 181.8 bits (460), Expect = 6.1e-42
Identity = 92/110 (83.64%), Postives = 99/110 (90.00%), Query Frame = 0 

Query: 1   MACNSSACQSNCYRNQDDEETVEQDSPPTTKPDTAAAVESRRVDTNDDSSNTHLCLKCKS 60
           MACNSSACQSNCYRNQ DE T+EQDSPP+++P TAA  ESR   TNDD+SNT+LC KCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGESR--ITNDDNSNTNLCFKCKS 60

Query: 61  KERMSSSGVGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSG 111
           KERMS+SGVGDDGR CSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSG
Sbjct: 61  KERMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSG 108

[Close]
BLAST of Lag0026428 vs. NCBI nr
Match: XP_022152059.1 (cytoplasmic tRNA 2-thiolation protein 2 [Momordica charantia])

HSP 1 Score: 177.2 bits (448), Expect = 1.5e-40
Identity = 91/110 (82.73%), Postives = 94/110 (85.45%), Query Frame = 0 

Query: 1   MACNSSACQSNCYRNQDDEETVEQDSPPTTKPDTAAAVESRRVDTNDDSSNTHLCLKCKS 60
           MACNSS CQSNCYRNQDDEET+EQ SPP  K + AAA+ES  VDTN D  NTHLC KCKS
Sbjct: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60

Query: 61  KERMSSSGVGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSG 111
            ERMSSSGVGDDGRFCS CFRSNLFAKFR AV SNAMISPSDKVLVAFSG
Sbjct: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSG 109

[Close]
BLAST of Lag0026428 vs. NCBI nr
Match: XP_022939604.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita moschata])

HSP 1 Score: 169.1 bits (427), Expect = 4.1e-38
Identity = 89/110 (80.91%), Postives = 91/110 (82.73%), Query Frame = 0 

Query: 1   MACNSSACQSNCYRNQDDEETVEQDSPPTTKPDTAAAVESRRVDTNDDSSNTHLCLKCKS 60
           MACNSSACQSNCYRNQDDE TVEQDS  T K D  AA       TN DS +THLC KCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 60

Query: 61  KERMSSSGVGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSG 111
           KERMSSS  GDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSG
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSG 103

[Close]
BLAST of Lag0026428 vs. ExPASy Swiss-Prot
Match: O65628 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Arabidopsis thaliana OX=3702 GN=CTU2 PE=2 SV=3)

HSP 1 Score: 87.4 bits (215), Expect = 2.0e-16
Identity = 50/112 (44.64%), Postives = 66/112 (58.93%), Query Frame = 0 

Query: 1   MACNSSACQSNCY-RNQDDEETVEQDSPPTTKPDTAAAVESRRVDTNDDSSNTHLCLKCK 60
           MACNSS C+S CY R +D+   +  D+                   +   ++  +C+KCK
Sbjct: 1   MACNSSGCESGCYDREKDNGSKIVDDA------------------VSGGGNHESVCVKCK 60

Query: 61  SKERMS-SSGVGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSG 111
               M+   G  DDGRFC+DCFR+N+F KFR AV S+AMI+PSD VLVAFSG
Sbjct: 61  CNAPMTFGDGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSG 94

[Close]
BLAST of Lag0026428 vs. ExPASy Swiss-Prot
Match: A2ZM73 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=CTU2 PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 9.5e-06
Identity = 32/85 (37.65%), Postives = 44/85 (51.76%), Query Frame = 0 

Query: 27  PPTTKPDTAAAVESRRVDTNDDSSNTHLCLKC-KSKERMSSSGVGDDGRFCSDCFRSNLF 86
           P  +   +AAAVE       +      L  +C K     ++  V      C +CFR+NL 
Sbjct: 16  PHCSSSASAAAVEDAAAAAAEKVGRLSLSRECGKCGGGAAAVAVAGGLGLCGECFRANLL 75

Query: 87  AKFRQAVNSNAMISPSDKVLVAFSG 111
            KF+ AV SNAM+ P+D VL+AFSG
Sbjct: 76  GKFKLAVTSNAMVRPTDSVLLAFSG 100

[Close]
BLAST of Lag0026428 vs. ExPASy Swiss-Prot
Match: Q2QMW0 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CTU2 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 9.5e-06
Identity = 32/85 (37.65%), Postives = 44/85 (51.76%), Query Frame = 0 

Query: 27  PPTTKPDTAAAVESRRVDTNDDSSNTHLCLKC-KSKERMSSSGVGDDGRFCSDCFRSNLF 86
           P  +   +AAAVE       +      L  +C K     ++  V      C +CFR+NL 
Sbjct: 16  PHCSSSASAAAVEDAAAAAAEKVGRLSLSRECGKCGGGAAAVAVAGGLGLCGECFRANLL 75

Query: 87  AKFRQAVNSNAMISPSDKVLVAFSG 111
            KF+ AV SNAM+ P+D VL+AFSG
Sbjct: 76  GKFKLAVTSNAMVRPTDSVLLAFSG 100

[Close]
BLAST of Lag0026428 vs. ExPASy Swiss-Prot
Match: Q28ES8 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Xenopus tropicalis OX=8364 GN=ctu2 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 8.1e-05
Identity = 32/96 (33.33%), Postives = 42/96 (43.75%), Query Frame = 0 

Query: 44  DTNDDSSNTHLCLKCKSKERMSSSGVGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDK 103
           DT    S    C+KCK    +     GD   FC  CF+     KFR  +  N +I P +K
Sbjct: 14  DTTQGKSLGKTCMKCKESSAVLLIRAGD--AFCKSCFKEYFVHKFRATLGKNRVIYPGEK 73

Query: 104 VLVAFSGVLVPVVKPWTRANAQQSQLNANNSSSSSL 140
           VL+AFSG       P + A  QQ Q   +  +   L
Sbjct: 74  VLLAFSG------GPSSSAMVQQVQEGMSRDAPKKL 101

[Close]
BLAST of Lag0026428 vs. ExPASy Swiss-Prot
Match: Q2VPK5 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Homo sapiens OX=9606 GN=CTU2 PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 1.8e-04
Identity = 30/99 (30.30%), Postives = 44/99 (44.44%), Query Frame = 0 

Query: 12  CYRNQDDEETVEQDSPPTTKPDTAAAVESRRVDTNDDSSNTHLCLKCKSKERMSSSGVGD 71
           C   +D  E   ++ PP  +P                 S    C+KCK  + +     GD
Sbjct: 2   CQVGEDYGEPAPEEPPPAPRP-----------------SREQKCVKCKEAQPVVVIRAGD 61

Query: 72  DGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSG 111
              FC DCF++    KFR  +  N +I P +KVL+A+SG
Sbjct: 62  --AFCRDCFKAFYVHKFRAMLGKNRLIFPGEKVLLAWSG 81

[Close]
BLAST of Lag0026428 vs. ExPASy TrEMBL
Match: A0A0A0LC57 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis sativus OX=3659 GN=Csa_3G816060 PE=3 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 1.3e-45
Identity = 101/141 (71.63%), Postives = 116/141 (82.27%), Query Frame = 0 

Query: 1   MACNSSACQSNCYRNQDDEETVEQDSPPTTKPDTAAAVESRRVDTNDDSSNTHLCLKCKS 60
           MACNSSACQSNCYRNQ DE T+EQDSPP+++PDT AA+E+RRV T DD+SNT+LC KCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60

Query: 61  KERMSSSGVGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGVLVPVVKPWT 120
           KERMS+SGVGDDGRFCSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSG       P +
Sbjct: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSG------GPCS 120

Query: 121 RANAQ---QSQLNANNSSSSS 139
           R   Q   + Q+ A+N+  +S
Sbjct: 121 RVALQFVHEMQVKAHNNFEAS 135

[Close]
BLAST of Lag0026428 vs. ExPASy TrEMBL
Match: A0A1S3B7H9 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo OX=3656 GN=LOC103486848 PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 2.9e-42
Identity = 92/110 (83.64%), Postives = 99/110 (90.00%), Query Frame = 0 

Query: 1   MACNSSACQSNCYRNQDDEETVEQDSPPTTKPDTAAAVESRRVDTNDDSSNTHLCLKCKS 60
           MACNSSACQSNCYRNQ DE T+EQDSPP+++P TAA  ESR   TNDD+SNT+LC KCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGESR--ITNDDNSNTNLCFKCKS 60

Query: 61  KERMSSSGVGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSG 111
           KERMS+SGVGDDGR CSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSG
Sbjct: 61  KERMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSG 108

[Close]
BLAST of Lag0026428 vs. ExPASy TrEMBL
Match: A0A5D3DPK4 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006240 PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 2.9e-42
Identity = 92/110 (83.64%), Postives = 99/110 (90.00%), Query Frame = 0 

Query: 1   MACNSSACQSNCYRNQDDEETVEQDSPPTTKPDTAAAVESRRVDTNDDSSNTHLCLKCKS 60
           MACNSSACQSNCYRNQ DE T+EQDSPP+++P TAA  ESR   TNDD+SNT+LC KCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGESR--ITNDDNSNTNLCFKCKS 60

Query: 61  KERMSSSGVGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSG 111
           KERMS+SGVGDDGR CSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSG
Sbjct: 61  KERMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSG 108

[Close]
BLAST of Lag0026428 vs. ExPASy TrEMBL
Match: A0A6J1DEW9 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Momordica charantia OX=3673 GN=LOC111019863 PE=3 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 7.3e-41
Identity = 91/110 (82.73%), Postives = 94/110 (85.45%), Query Frame = 0 

Query: 1   MACNSSACQSNCYRNQDDEETVEQDSPPTTKPDTAAAVESRRVDTNDDSSNTHLCLKCKS 60
           MACNSS CQSNCYRNQDDEET+EQ SPP  K + AAA+ES  VDTN D  NTHLC KCKS
Sbjct: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60

Query: 61  KERMSSSGVGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSG 111
            ERMSSSGVGDDGRFCS CFRSNLFAKFR AV SNAMISPSDKVLVAFSG
Sbjct: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSG 109

[Close]
BLAST of Lag0026428 vs. ExPASy TrEMBL
Match: A0A6J1FHP7 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111445447 PE=3 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 2.0e-38
Identity = 89/110 (80.91%), Postives = 91/110 (82.73%), Query Frame = 0 

Query: 1   MACNSSACQSNCYRNQDDEETVEQDSPPTTKPDTAAAVESRRVDTNDDSSNTHLCLKCKS 60
           MACNSSACQSNCYRNQDDE TVEQDS  T K D  AA       TN DS +THLC KCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 60

Query: 61  KERMSSSGVGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSG 111
           KERMSSS  GDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSG
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSG 103

[Close]
BLAST of Lag0026428 vs. TAIR 10
Match: AT4G35910.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 87.4 bits (215), Expect = 1.5e-17
Identity = 50/112 (44.64%), Postives = 66/112 (58.93%), Query Frame = 0 

Query: 1   MACNSSACQSNCY-RNQDDEETVEQDSPPTTKPDTAAAVESRRVDTNDDSSNTHLCLKCK 60
           MACNSS C+S CY R +D+   +  D+                   +   ++  +C+KCK
Sbjct: 1   MACNSSGCESGCYDREKDNGSKIVDDA------------------VSGGGNHESVCVKCK 60

Query: 61  SKERMS-SSGVGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSG 111
               M+   G  DDGRFC+DCFR+N+F KFR AV S+AMI+PSD VLVAFSG
Sbjct: 61  CNAPMTFGDGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSG 94

The following BLAST results are available for this feature:
BLAST of Lag0026428 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004136614.12.6e-4571.63cytoplasmic tRNA 2-thiolation protein 2 [Cucumis sativus] >XP_011652154.1 cytopl... [more]
XP_038905927.14.5e-4586.36cytoplasmic tRNA 2-thiolation protein 2 [Benincasa hispida][more]
XP_008443191.16.1e-4283.64PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo] >XP_008443192.... [more]
XP_022152059.11.5e-4082.73cytoplasmic tRNA 2-thiolation protein 2 [Momordica charantia][more]
XP_022939604.14.1e-3880.91cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita moschata][more]
BLAST of Lag0026428 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O656282.0e-1644.64Cytoplasmic tRNA 2-thiolation protein 2 OS=Arabidopsis thaliana OX=3702 GN=CTU2 ... [more]
A2ZM739.5e-0637.65Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. indica OX=39946 G... [more]
Q2QMW09.5e-0637.65Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. japonica OX=39947... [more]
Q28ES88.1e-0533.33Cytoplasmic tRNA 2-thiolation protein 2 OS=Xenopus tropicalis OX=8364 GN=ctu2 PE... [more]
Q2VPK51.8e-0430.30Cytoplasmic tRNA 2-thiolation protein 2 OS=Homo sapiens OX=9606 GN=CTU2 PE=1 SV=... [more]
BLAST of Lag0026428 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LC571.3e-4571.63Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis sativus OX=3659 GN=Csa_3G8160... [more]
A0A1S3B7H92.9e-4283.64Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo OX=3656 GN=LOC103486848 ... [more]
A0A5D3DPK42.9e-4283.64Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A6J1DEW97.3e-4182.73Cytoplasmic tRNA 2-thiolation protein 2 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1FHP72.0e-3880.91Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
BLAST of Lag0026428 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G35910.11.5e-1744.64Adenine nucleotide alpha hydrolases-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 54..111
e-value: 3.7E-10
score: 41.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..15
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..50
IPR019407Cytoplasmic tRNA 2-thiolation protein 2PANTHERPTHR20882CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2coord: 47..110

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0026428.1Lag0026428.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010311 lateral root formation
biological_process GO:0032447 protein urmylation
biological_process GO:0002143 tRNA wobble position uridine thiolation
biological_process GO:0034227 tRNA thio-modification
biological_process GO:0002098 tRNA wobble uridine modification
cellular_component GO:0005829 cytosol
molecular_function GO:0016779 nucleotidyltransferase activity
molecular_function GO:0016783 sulfurtransferase activity
molecular_function GO:0000049 tRNA binding