Lag0026294 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0026294
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Werner Syndrome-like exonuclease
Location	chr10: 34067798 .. 34068430 (-)
RNA-Seq Expression	Lag0026294
Synteny	Lag0026294

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTACTCTCAGCATTTCTGACCGCCAAATCCCCTTCGACTCCCACGACTACTACGACATTTCTTTCGACGACGACGACATTCTCACCCTCCTCACTGCCTGCCCTTCCATGGTCGACCTTTGGATCTCCGAGATCCTCCGCATCCACCGCCGCCGCCTCCACCGTCTCGTCGTCGGCCTCGACATCGAGTGGCGCCCTAACTTCAATCGCAATCACCACAACCCCGTCGCCACTCTCCAGCTCTGCGTCGGCCGTCGCTGCCTCATCTTCCAACTCATCCACGCTCCGGAGATCCCCGACTCTTTGATCGAGTTCTTGGAGGACGAATCCTTCACCTTCGTCGGCGTCGGAGTCGACCAGGACGCTCGGCGCCTCCAGAACGAGTACGGCTTGAATGTGGCCAACACTGCGGATCTTAGGGATTTGGCGGAGAGTGAGATGGGGAGAAGAGATCTTAGAAATGCCGGACTGAAGGATTTGGGGAGGGAGATTCTGGGAAGGGAGATTAGCAAGCCGAGAAATGTGACGCTGAGTCGGTGGGATCGTGAATGGCTGAATCCTGCTCAGGTACTCTATGCTACTGTTGATGCTTTCTTGTCTTTTGAGATCGGAAGGTATCTGATGAGTTAG

mRNA sequence

ATGGCTACTCTCAGCATTTCTGACCGCCAAATCCCCTTCGACTCCCACGACTACTACGACATTTCTTTCGACGACGACGACATTCTCACCCTCCTCACTGCCTGCCCTTCCATGGTCGACCTTTGGATCTCCGAGATCCTCCGCATCCACCGCCGCCGCCTCCACCGTCTCGTCGTCGGCCTCGACATCGAGTGGCGCCCTAACTTCAATCGCAATCACCACAACCCCGTCGCCACTCTCCAGCTCTGCGTCGGCCGTCGCTGCCTCATCTTCCAACTCATCCACGCTCCGGAGATCCCCGACTCTTTGATCGAGTTCTTGGAGGACGAATCCTTCACCTTCGTCGGCGTCGGAGTCGACCAGGACGCTCGGCGCCTCCAGAACGAGTACGGCTTGAATGTGGCCAACACTGCGGATCTTAGGGATTTGGCGGAGAGTGAGATGGGGAGAAGAGATCTTAGAAATGCCGGACTGAAGGATTTGGGGAGGGAGATTCTGGGAAGGGAGATTAGCAAGCCGAGAAATGTGACGCTGAGTCGGTGGGATCGTGAATGGCTGAATCCTGCTCAGGTACTCTATGCTACTGTTGATGCTTTCTTGTCTTTTGAGATCGGAAGGTATCTGATGAGTTAG

Coding sequence (CDS)

ATGGCTACTCTCAGCATTTCTGACCGCCAAATCCCCTTCGACTCCCACGACTACTACGACATTTCTTTCGACGACGACGACATTCTCACCCTCCTCACTGCCTGCCCTTCCATGGTCGACCTTTGGATCTCCGAGATCCTCCGCATCCACCGCCGCCGCCTCCACCGTCTCGTCGTCGGCCTCGACATCGAGTGGCGCCCTAACTTCAATCGCAATCACCACAACCCCGTCGCCACTCTCCAGCTCTGCGTCGGCCGTCGCTGCCTCATCTTCCAACTCATCCACGCTCCGGAGATCCCCGACTCTTTGATCGAGTTCTTGGAGGACGAATCCTTCACCTTCGTCGGCGTCGGAGTCGACCAGGACGCTCGGCGCCTCCAGAACGAGTACGGCTTGAATGTGGCCAACACTGCGGATCTTAGGGATTTGGCGGAGAGTGAGATGGGGAGAAGAGATCTTAGAAATGCCGGACTGAAGGATTTGGGGAGGGAGATTCTGGGAAGGGAGATTAGCAAGCCGAGAAATGTGACGCTGAGTCGGTGGGATCGTGAATGGCTGAATCCTGCTCAGGTACTCTATGCTACTGTTGATGCTTTCTTGTCTTTTGAGATCGGAAGGTATCTGATGAGTTAG

Protein sequence

MATLSISDRQIPFDSHDYYDISFDDDDILTLLTACPSMVDLWISEILRIHRRRLHRLVVGLDIEWRPNFNRNHHNPVATLQLCVGRRCLIFQLIHAPEIPDSLIEFLEDESFTFVGVGVDQDARRLQNEYGLNVANTADLRDLAESEMGRRDLRNAGLKDLGREILGREISKPRNVTLSRWDREWLNPAQVLYATVDAFLSFEIGRYLMS

Homology

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BLAST of Lag0026294 vs. NCBI nr
Match: XP_004136771.1 (Werner Syndrome-like exonuclease [Cucumis sativus])

HSP 1 Score: 350.1 bits (897), Expect = 1.3e-92
Identity = 166/210 (79.05%), Postives = 187/210 (89.05%), Query Frame = 0


Query: 1   MATLSISDRQIPFDSHDYYDISFDDDDILTLLTACPSMVDLWISEILRIHRRRLHRLVVG 60
           MATL+ISDRQIP +SHDYYDI+FDDD+ILTL+TA PSMVD+WISEILRIHRRRLHRLVVG
Sbjct: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDIEWRPNFNRNHHNPVATLQLCVGRRCLIFQLIHAPEIPDSLIEFLEDESFTFVGVGVD 120
           LD+EWRPNF+R+  NPVATLQLCVGRRCLIFQLIHA E P SLI+FLED++FTFVGVG+D
Sbjct: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120

Query: 121 QDARRLQNEYGLNVANTADLRDLAESEMGRRDLRNAGLKDLGREILGREISKPRNVTLSR 180
            D  +L N+Y LNVANT DLR+LA  EM   + R AGLK LGRE+LGREI KPRNV LSR
Sbjct: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180

Query: 181 WDREWLNPAQVLYATVDAFLSFEIGRYLMS 211
           WDR+WLNPAQ+LYATVDAFLSFEIGRYL+S
Sbjct: 181 WDRQWLNPAQILYATVDAFLSFEIGRYLLS 210

[Close]

BLAST of Lag0026294 vs. NCBI nr
Match: XP_038906336.1 (Werner Syndrome-like exonuclease [Benincasa hispida])

HSP 1 Score: 346.3 bits (887), Expect = 1.9e-91
Identity = 167/210 (79.52%), Postives = 189/210 (90.00%), Query Frame = 0


Query: 1   MATLSISDRQIPFDSHDYYDISFDDDDILTLLTACPSMVDLWISEILRIHRRRLHRLVVG 60
           MA LSISDRQIP +SHDYYDI+FD+D ILTLLTAC S+VD+WISEILRIHRRRLHRLVVG
Sbjct: 1   MAILSISDRQIPLNSHDYYDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDIEWRPNFNRNHHNPVATLQLCVGRRCLIFQLIHAPEIPDSLIEFLEDESFTFVGVGVD 120
           LDIEWRPNFNR +  PVATLQLCVGRRCLIFQLIH  EIP+SLI+FLED++FTFVGVG+D
Sbjct: 61  LDIEWRPNFNRRYRKPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTFTFVGVGID 120

Query: 121 QDARRLQNEYGLNVANTADLRDLAESEMGRRDLRNAGLKDLGREILGREISKPRNVTLSR 180
           QD ++L N+Y L VANT DLR+LA +EM R DL+NAGLK LGRE+LGREI KPRNVTLS+
Sbjct: 121 QDVQQLYNDYDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNVTLSQ 180

Query: 181 WDREWLNPAQVLYATVDAFLSFEIGRYLMS 211
           WDR+ L+P QVLYATVDAFLSFEIGRYL+S
Sbjct: 181 WDRQCLSPGQVLYATVDAFLSFEIGRYLLS 210

[Close]

BLAST of Lag0026294 vs. NCBI nr
Match: KAE8651170.1 (hypothetical protein Csa_000755 [Cucumis sativus])

HSP 1 Score: 344.0 bits (881), Expect = 9.3e-91
Identity = 163/206 (79.13%), Postives = 183/206 (88.83%), Query Frame = 0


Query: 1   MATLSISDRQIPFDSHDYYDISFDDDDILTLLTACPSMVDLWISEILRIHRRRLHRLVVG 60
           MATL+ISDRQIP +SHDYYDI+FDDD+ILTL+TA PSMVD+WISEILRIHRRRLHRLVVG
Sbjct: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDIEWRPNFNRNHHNPVATLQLCVGRRCLIFQLIHAPEIPDSLIEFLEDESFTFVGVGVD 120
           LD+EWRPNF+R+  NPVATLQLCVGRRCLIFQLIHA E P SLI+FLED++FTFVGVG+D
Sbjct: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120

Query: 121 QDARRLQNEYGLNVANTADLRDLAESEMGRRDLRNAGLKDLGREILGREISKPRNVTLSR 180
            D  +L N+Y LNVANT DLR+LA  EM   + R AGLK LGRE+LGREI KPRNV LSR
Sbjct: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180

Query: 181 WDREWLNPAQVLYATVDAFLSFEIGR 207
           WDR+WLNPAQ+LYATVDAFLSFEIGR
Sbjct: 181 WDRQWLNPAQILYATVDAFLSFEIGR 206

[Close]

BLAST of Lag0026294 vs. NCBI nr
Match: XP_022147765.1 (Werner Syndrome-like exonuclease, partial [Momordica charantia])

HSP 1 Score: 293.1 bits (749), Expect = 1.9e-75
Identity = 141/197 (71.57%), Postives = 162/197 (82.23%), Query Frame = 0


Query: 14  DSHDYYDISFDDDDILTLLTACPSMVDLWISEILRIHRRRLHRLVVGLDIEWRPNFNRNH 73
           +SH+ YDI+FD D I T +T+ P MVD WIS ILR HRRRLHRL+VGLD+EWRPNFNR+ 
Sbjct: 5   NSHNCYDITFDSDQIKTTITSSPYMVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSR 64

Query: 74  HNPVATLQLCVGRRCLIFQLIHAPEIPDSLIEFLEDESFTFVGVGVDQDARRLQNEYGLN 133
            NP ATLQLCVGRRCLIFQLIHAPEIP SL+ FL DE+FTFVGVG+DQDAR+L ++Y L 
Sbjct: 65  RNPAATLQLCVGRRCLIFQLIHAPEIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLT 124

Query: 134 VANTADLRDLAESEMGRRDLRNAGLKDLGREILGREISKPRNVTLSRWDREWLNPAQVLY 193
           V N  DLR +A    GRRDLRNAGLKDL REILGREI+KP+ VT+SRWD E+L  AQV Y
Sbjct: 125 VNNAVDLRSMAVDRTGRRDLRNAGLKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAY 184

Query: 194 ATVDAFLSFEIGRYLMS 211
           A++DAFLSFEIGR L S
Sbjct: 185 ASIDAFLSFEIGRSLSS 201

[Close]

BLAST of Lag0026294 vs. NCBI nr
Match: KAB1222804.1 (Werner Syndrome-like exonuclease [Morella rubra])

HSP 1 Score: 288.1 bits (736), Expect = 6.1e-74
Identity = 139/208 (66.83%), Postives = 167/208 (80.29%), Query Frame = 0


Query: 1   MATLSISDRQIPFDSHDYYDISFDDDDILTLLTACPSMVDLWISEILRIHRRRLHRLVVG 60
           MA++SI D Q+P ++++ Y +S D DD+LTL+T  PSMVD WIS+I RIH RRLHRLVVG
Sbjct: 1   MASISIFDHQLPDNTYNLYVVSLDSDDVLTLVTHSPSMVDSWISDIYRIHSRRLHRLVVG 60

Query: 61  LDIEWRPNFNRNHHNPVATLQLCVGRRCLIFQLIHAPEIPDSLIEFLEDESFTFVGVGVD 120
           LD+EWRP+FNR+  NPVATLQLCVGRRCLIFQ++ AP  PDSLIEFL D SFTFVGVG++
Sbjct: 61  LDVEWRPSFNRHVQNPVATLQLCVGRRCLIFQIVRAPYFPDSLIEFLGDGSFTFVGVGIE 120

Query: 121 QDARRLQNEYGLNVANTADLRDLAESEMGRRDLRNAGLKDLGREILGREISKPRNVTLSR 180
            D  +L ++Y L VAN  DLRDL E E G R+LRNAGLK L  E+LG++I KPR VTLSR
Sbjct: 121 SDVEKLLDDYELEVANVVDLRDLVEEEWGIRELRNAGLKQLAWEVLGKDIQKPRRVTLSR 180

Query: 181 WDREWLNPAQVLYATVDAFLSFEIGRYL 209
           WD E L+ AQV YA +DAFLSFEIGR+L
Sbjct: 181 WDNERLSHAQVQYACLDAFLSFEIGRFL 208

[Close]

BLAST of Lag0026294 vs. ExPASy Swiss-Prot
Match: Q84LH3 (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 3.1e-20
Identity = 54/152 (35.53%), Postives = 88/152 (57.89%), Query Frame = 0


Query: 59  VGLDIEWRPNFNRN-HHNPVATLQLCV-GRRCLIFQLIHAPEIPDSLIEFLEDESFTFVG 118
           VGLDIEWRP+F +      VAT+Q+CV    C +  + H+  IP SL   +ED +   VG
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHS-GIPQSLQHLIEDSTLVKVG 189

Query: 119 VGVDQDARRLQNEYGLNVANTADLRDLAESEMGRRDLRNAGLKDLGREILGREISKPRNV 178
           +G+D D+ +L ++YG+++ +  DL DLA  ++G    +  GL  L   ++ +E+ KP  +
Sbjct: 190 IGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGLASLTETLVCKELLKPNRI 249

Query: 179 TLSRWDREWLNPAQVLYATVDAFLSFEIGRYL 209
            L  W+   L+  Q+ YA  DA+ S+ + + L
Sbjct: 250 RLGNWEFYPLSKQQLQYAATDAYASWHLYKVL 278

[Close]

BLAST of Lag0026294 vs. ExPASy Swiss-Prot
Match: Q9VGN7 (Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227 GN=Exd2 PE=2 SV=2)

HSP 1 Score: 84.7 bits (208), Expect = 1.3e-15
Identity = 57/164 (34.76%), Postives = 81/164 (49.39%), Query Frame = 0


Query: 42  WISEILRIHRRRLHRLVVGLDIEWRPNFNRNHHNPVATLQLCVGR-RCLIFQLIHAPEIP 101
           W+   L+ H +     V+G D EW          PVA LQL   R  C +F+L H  +IP
Sbjct: 65  WVLNELKNHCQTFK--VLGFDCEWIT--VGGSRRPVALLQLSSHRGLCALFRLCHMKQIP 124

Query: 102 DSLIEFLEDESFTFVGVGVDQDARRLQNEYGLNVANTADLRDLAESEMGRRDLRNAGLKD 161
             L E LED+S   VGV   +DA +L ++YG+ VA+T DLR L      + +    GL  
Sbjct: 125 QDLRELLEDDSVIKVGVAPQEDAMKLSHDYGVGVASTLDLRFLCVMAGHKPE----GLGK 184

Query: 162 LGREILGREISKPRNVTLSRWDREWLNPAQVLYATVDAFLSFEI 205
           L +  L   + K   +  S W+ + L P Q+ YA  DA ++  I
Sbjct: 185 LSKTHLNYTLDKHWRLACSNWEAKTLEPKQLDYAANDALMAVAI 220

[Close]

BLAST of Lag0026294 vs. ExPASy Swiss-Prot
Match: O93530 (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn PE=2 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 3.3e-14
Identity = 46/152 (30.26%), Postives = 74/152 (48.68%), Query Frame = 0


Query: 58  VVGLDIEWRPNFNRNHHNPVATLQLCVG-RRCLIFQLIHAPEIPDSLIEFLEDESFTFVG 117
           V+G DIEW P + +     VA +Q+CV  ++C +F +      P  L   LEDES   VG
Sbjct: 72  VLGFDIEWPPVYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVG 131

Query: 118 VGVDQDARRLQNEYGLNVANTADLRDLAESEMGRRDLRNAGLKDLGREILGREISKPRNV 177
           VG++ D  +L ++Y L +    +L ++A  ++  R         L + +   ++ K ++ 
Sbjct: 132 VGIEGDQWKLMSDYELKLKGFIELSEMANQKL--RCKEKWTFNGLIKHLFKEQLYKRKSY 191

Query: 178 TLSRWDREWLNPAQVLYATVDAFLSFEIGRYL 209
             S WD   L   Q LYA  DA+    I + L
Sbjct: 192 RCSNWDIFLLTEDQKLYAATDAYAGLLIYKKL 221

[Close]

BLAST of Lag0026294 vs. ExPASy Swiss-Prot
Match: Q9NVH0 (Exonuclease 3'-5' domain-containing protein 2 OS=Homo sapiens OX=9606 GN=EXD2 PE=1 SV=2)

HSP 1 Score: 71.6 bits (174), Expect = 1.2e-11
Identity = 49/157 (31.21%), Postives = 75/157 (47.77%), Query Frame = 0


Query: 58  VVGLDIEWRPNFNRNHHNPVATLQLC-VGRRCLIFQL----IHAPEIPDSLIEFLEDESF 117
           V+G+D EW  N      +P++ LQ+      C++ +L         +P +L++ L D + 
Sbjct: 104 VLGIDCEW-VNL-EGKASPLSLLQMASPSGLCVLVRLPKLICGGKTLPRTLLDILADGTI 163

Query: 118 TFVGVGVDQDARRLQNEYGLNVANTADLRDLAESEMGRRDLRNAGLKDLGREILGREISK 177
             VGVG  +DA +L  +YGL V    DLR LA  +          LK L   +L   + K
Sbjct: 164 LKVGVGCSEDASKLLQDYGLVVRGCLDLRYLAMRQRNNLLCNGLSLKSLAETVLNFPLDK 223

Query: 178 PRNVTLSRWDREWLNPAQVLYATVDAFLSFEIGRYLM 210
              +  S WD E L   QV+YA  DA +S  +  +L+
Sbjct: 224 SLLLRCSNWDAETLTEDQVIYAARDAQISVALFLHLL 258

[Close]

BLAST of Lag0026294 vs. ExPASy Swiss-Prot
Match: Q8VEG4 (Exonuclease 3'-5' domain-containing protein 2 OS=Mus musculus OX=10090 GN=Exd2 PE=1 SV=2)

HSP 1 Score: 68.2 bits (165), Expect = 1.3e-10
Identity = 50/157 (31.85%), Postives = 73/157 (46.50%), Query Frame = 0


Query: 58  VVGLDIEWRPNFNRNHHNPVATLQLC-VGRRCLIFQL----IHAPEIPDSLIEFLEDESF 117
           V+G+D EW  N      +P++ LQ+      C + +L         +P +L++ L D + 
Sbjct: 133 VLGIDCEW-VNL-EGKASPLSLLQMASPSGFCALVRLPRLIYGGRTLPRTLLDILADGAI 192

Query: 118 TFVGVGVDQDARRLQNEYGLNVANTADLRDLAESEMGRRDLRNAGLKDLGREILGREISK 177
             VGVG  +DA +L  +YGL V    DLR LA  +          LK L   IL   + K
Sbjct: 193 LKVGVGCSEDANKLLQDYGLIVRGCLDLRYLAMKQGNNILCNGLSLKSLAETILNFPLDK 252

Query: 178 PRNVTLSRWDREWLNPAQVLYATVDAFLSFEIGRYLM 210
              +  S WD E L   QV YA  DA +S  +  +L+
Sbjct: 253 SLLLRCSNWDAENLTEDQVTYAARDAQISVALFLHLL 287

[Close]

BLAST of Lag0026294 vs. ExPASy TrEMBL
Match: A0A0A0LHC5 (3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G819830 PE=4 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 6.3e-93
Identity = 166/210 (79.05%), Postives = 187/210 (89.05%), Query Frame = 0


Query: 1   MATLSISDRQIPFDSHDYYDISFDDDDILTLLTACPSMVDLWISEILRIHRRRLHRLVVG 60
           MATL+ISDRQIP +SHDYYDI+FDDD+ILTL+TA PSMVD+WISEILRIHRRRLHRLVVG
Sbjct: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDIEWRPNFNRNHHNPVATLQLCVGRRCLIFQLIHAPEIPDSLIEFLEDESFTFVGVGVD 120
           LD+EWRPNF+R+  NPVATLQLCVGRRCLIFQLIHA E P SLI+FLED++FTFVGVG+D
Sbjct: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120

Query: 121 QDARRLQNEYGLNVANTADLRDLAESEMGRRDLRNAGLKDLGREILGREISKPRNVTLSR 180
            D  +L N+Y LNVANT DLR+LA  EM   + R AGLK LGRE+LGREI KPRNV LSR
Sbjct: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180

Query: 181 WDREWLNPAQVLYATVDAFLSFEIGRYLMS 211
           WDR+WLNPAQ+LYATVDAFLSFEIGRYL+S
Sbjct: 181 WDRQWLNPAQILYATVDAFLSFEIGRYLLS 210

[Close]

BLAST of Lag0026294 vs. ExPASy TrEMBL
Match: A0A6J1D121 (Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016622 PE=4 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 9.1e-76
Identity = 141/197 (71.57%), Postives = 162/197 (82.23%), Query Frame = 0


Query: 14  DSHDYYDISFDDDDILTLLTACPSMVDLWISEILRIHRRRLHRLVVGLDIEWRPNFNRNH 73
           +SH+ YDI+FD D I T +T+ P MVD WIS ILR HRRRLHRL+VGLD+EWRPNFNR+ 
Sbjct: 5   NSHNCYDITFDSDQIKTTITSSPYMVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSR 64

Query: 74  HNPVATLQLCVGRRCLIFQLIHAPEIPDSLIEFLEDESFTFVGVGVDQDARRLQNEYGLN 133
            NP ATLQLCVGRRCLIFQLIHAPEIP SL+ FL DE+FTFVGVG+DQDAR+L ++Y L 
Sbjct: 65  RNPAATLQLCVGRRCLIFQLIHAPEIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLT 124

Query: 134 VANTADLRDLAESEMGRRDLRNAGLKDLGREILGREISKPRNVTLSRWDREWLNPAQVLY 193
           V N  DLR +A    GRRDLRNAGLKDL REILGREI+KP+ VT+SRWD E+L  AQV Y
Sbjct: 125 VNNAVDLRSMAVDRTGRRDLRNAGLKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAY 184

Query: 194 ATVDAFLSFEIGRYLMS 211
           A++DAFLSFEIGR L S
Sbjct: 185 ASIDAFLSFEIGRSLSS 201

[Close]

BLAST of Lag0026294 vs. ExPASy TrEMBL
Match: A0A6A1WBZ0 (Werner Syndrome-like exonuclease OS=Morella rubra OX=262757 GN=CJ030_MR2G022295 PE=4 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 2.9e-74
Identity = 139/208 (66.83%), Postives = 167/208 (80.29%), Query Frame = 0


Query: 1   MATLSISDRQIPFDSHDYYDISFDDDDILTLLTACPSMVDLWISEILRIHRRRLHRLVVG 60
           MA++SI D Q+P ++++ Y +S D DD+LTL+T  PSMVD WIS+I RIH RRLHRLVVG
Sbjct: 1   MASISIFDHQLPDNTYNLYVVSLDSDDVLTLVTHSPSMVDSWISDIYRIHSRRLHRLVVG 60

Query: 61  LDIEWRPNFNRNHHNPVATLQLCVGRRCLIFQLIHAPEIPDSLIEFLEDESFTFVGVGVD 120
           LD+EWRP+FNR+  NPVATLQLCVGRRCLIFQ++ AP  PDSLIEFL D SFTFVGVG++
Sbjct: 61  LDVEWRPSFNRHVQNPVATLQLCVGRRCLIFQIVRAPYFPDSLIEFLGDGSFTFVGVGIE 120

Query: 121 QDARRLQNEYGLNVANTADLRDLAESEMGRRDLRNAGLKDLGREILGREISKPRNVTLSR 180
            D  +L ++Y L VAN  DLRDL E E G R+LRNAGLK L  E+LG++I KPR VTLSR
Sbjct: 121 SDVEKLLDDYELEVANVVDLRDLVEEEWGIRELRNAGLKQLAWEVLGKDIQKPRRVTLSR 180

Query: 181 WDREWLNPAQVLYATVDAFLSFEIGRYL 209
           WD E L+ AQV YA +DAFLSFEIGR+L
Sbjct: 181 WDNERLSHAQVQYACLDAFLSFEIGRFL 208

[Close]

BLAST of Lag0026294 vs. ExPASy TrEMBL
Match: A0A2I4GID7 (Werner Syndrome-like exonuclease OS=Juglans regia OX=51240 GN=LOC109008130 PE=4 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 5.0e-74
Identity = 138/205 (67.32%), Postives = 161/205 (78.54%), Query Frame = 0


Query: 4   LSISDRQIPFDSHDYYDISFDDDDILTLLTACPSMVDLWISEILRIHRRRLHRLVVGLDI 63
           +SI D ++PFD+H+ YD+SFD D I TLLT  PS+VD WIS+I RIHRRRLHRLVVGLD+
Sbjct: 3   ISIYDYELPFDTHNLYDVSFDSDQIGTLLTCSPSLVDSWISDIYRIHRRRLHRLVVGLDL 62

Query: 64  EWRPNFNRNHHNPVATLQLCVGRRCLIFQLIHAPEIPDSLIEFLEDESFTFVGVGVDQDA 123
           EWRP+FNR+  NPVATLQLCVGRRCLIFQLIHAP IP SL++FL    FTFVGVG+  D 
Sbjct: 63  EWRPSFNRHVQNPVATLQLCVGRRCLIFQLIHAPYIPQSLVDFLGQTDFTFVGVGIKSDV 122

Query: 124 RRLQNEYGLNVANTADLRDLAESEMGRRDLRNAGLKDLGREILGREISKPRNVTLSRWDR 183
            +L ++YGL VA   DLR LA  E+G   LRNAGLK L  E+LG+ I KPRN+T+SRWD 
Sbjct: 123 EKLLDDYGLEVACVVDLRLLAVEELGTTQLRNAGLKQLAWEVLGKRIEKPRNITMSRWDN 182

Query: 184 EWLNPAQVLYATVDAFLSFEIGRYL 209
           EWL  AQV YA +DAFLSFEIGR L
Sbjct: 183 EWLTCAQVQYACLDAFLSFEIGRQL 207

[Close]

BLAST of Lag0026294 vs. ExPASy TrEMBL
Match: A0A5C7ILY4 (3'-5' exonuclease domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_005156 PE=4 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 9.4e-73
Identity = 132/206 (64.08%), Postives = 165/206 (80.10%), Query Frame = 0


Query: 3   TLSISDRQIPFDSHDYYDISFDDDDILTLLTACPSMVDLWISEILRIHRRRLHRLVVGLD 62
           ++SI D ++P+DSH+ YD++F DD ILTL+T  PSMVD WI+EI RIHRRRLH LVVG D
Sbjct: 2   SISIVDHELPYDSHNIYDVNFYDDQILTLVTHTPSMVDSWITEIERIHRRRLHHLVVGFD 61

Query: 63  IEWRPNFNRNHHNPVATLQLCVGRRCLIFQLIHAPEIPDSLIEFLEDESFTFVGVGVDQD 122
           +EWRPNF+RN  NPVATLQLCVGRRCLIFQLIHAP IP SL+ FL ++++TFVGVG+D+D
Sbjct: 62  VEWRPNFHRNQDNPVATLQLCVGRRCLIFQLIHAPNIPRSLVNFLLNQNYTFVGVGIDKD 121

Query: 123 ARRLQNEYGLNVANTADLRDLAESEMGRRDLRNAGLKDLGREILGREISKPRNVTLSRWD 182
             +L+ +Y + V NT DLR LA   +    LRNA LK L RE+LG EISKP+++T+SRWD
Sbjct: 122 VEKLEEDYNMRVGNTVDLRGLAAYCLEMVGLRNASLKGLAREVLGLEISKPKSITMSRWD 181

Query: 183 REWLNPAQVLYATVDAFLSFEIGRYL 209
            +WL+ AQ+ YA VDAF+SFEIGR L
Sbjct: 182 YQWLSAAQIQYACVDAFVSFEIGRML 207

[Close]

BLAST of Lag0026294 vs. TAIR 10
Match: AT3G12460.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 104.0 bits (258), Expect = 1.5e-22
Identity = 64/209 (30.62%), Postives = 100/209 (47.85%), Query Frame = 0


Query: 19  YDISFDDDDILTLLTACPSMVDLWISEILRIHRRRLHRLVVGLDIEWRPNFNRNHHNPVA 78
           Y + F  +++   +T  PS++  WI ++   +R   H LVVG+ ++W P+       PV+
Sbjct: 17  YSVDFFGEELFVTVTPDPSVIGQWIHDVFFHNRLSSHPLVVGVGVQWTPSGYHPASPPVS 76

Query: 79  --------------------------TLQLCVGRRCLIFQLIHAPEIPDSLIEFLEDESF 138
                                     TLQLCVG RC+I QL +   +P  L  FL D   
Sbjct: 77  YRSDSSPDSYRSDSPPVFYSSDPPADTLQLCVGNRCIIIQLRYCERVPQVLRNFLADRDN 136

Query: 139 TFVGVGVDQDARRLQ-NEYGLNVANTADLRDLAESEMGRRDLRNAGLKDLGREILGRE-I 198
           TFVG+   QDA +L+ + + L +A   DLR+      GRR + N  L+ +  E LG   +
Sbjct: 137 TFVGIWNSQDAGKLERSRHQLEIAELMDLREFVSDSSGRRSMYNYSLEKIVEENLGYPGV 196

Query: 199 SKPRNVTLSRWDREWLNPAQVLYATVDAF 200
              R V++S W    L+  Q+L A++D +
Sbjct: 197 RLDREVSMSDWRVYNLSYDQILQASIDVY 225

[Close]

BLAST of Lag0026294 vs. TAIR 10
Match: AT2G36110.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 4.4e-22
Identity = 65/193 (33.68%), Postives = 97/193 (50.26%), Query Frame = 0


Query: 16  HDYYDISFDDDDILTLLTACPSMVDLWISEILRIHR-RRLHRLVVGLDIEWRPNFNRNHH 75
           H+   I F  + ++  +T   S +  WI  I    R R  H LVVGLD++W P       
Sbjct: 14  HENRYIDFFGERLIVTVTHTTSTIRRWIHSIRFFSRLRSSHPLVVGLDVQWTPG---GSD 73

Query: 76  NPVATLQLCVGRRCLIFQLIHAPEIPDSLIEFLEDESFTFVGVGVDQDARRLQN-EYGLN 135
            P   LQLCVG RCLI QL H   IP+ L  FLEDE+ TFVGV   QD  +L+   + L 
Sbjct: 74  PPPDILQLCVGNRCLIIQLSHCKRIPEVLRSFLEDETITFVGVWNSQDQGKLERFRHQLE 133

Query: 136 VANTADLRDLAESEMGRRDLRNAGLKDLGREILG-REISKPRNVTLSRWDREWLNPAQVL 195
           +    D+R    +      L N+  + +  E LG + + K + + +S W    L+  Q++
Sbjct: 134 IWRLLDIRHYLPTR-----LLNSSFEKIVEECLGYKGVRKDKEICMSNWGARSLSHDQIV 193

Query: 196 YATVDAFLSFEIG 206
            A+ D ++  ++G
Sbjct: 194 QASDDVYVCCKLG 198

[Close]

BLAST of Lag0026294 vs. TAIR 10
Match: AT3G12430.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 101.7 bits (252), Expect = 7.5e-22
Identity = 63/209 (30.14%), Postives = 98/209 (46.89%), Query Frame = 0


Query: 19  YDISFDDDDILTLLTACPSMVDLWISEILRIHRRRLHRLVVGLDIEWRPN---------- 78
           Y + F  ++ + ++T  PS++  WI ++L  +R   H LVVG+ ++W P+          
Sbjct: 17  YLVDFFGEEFIVIVTPDPSVIGQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVR 76

Query: 79  ----------------FNRNHHNPVATLQLCVGRRCLIFQLIHAPEIPDSLIEFLEDESF 138
                             R    P  TLQLCVG RC+I QL H   +P  L  FL D  +
Sbjct: 77  YRSDSPPVRYRSDSPRVRRRFDPPADTLQLCVGNRCIIIQLSHCERVPQVLRNFLADRDY 136

Query: 139 TFVGVGVDQDARRL-QNEYGLNVANTADLRDLAESEMGRRDLRNAGLKDLGREILGRE-I 198
           TFVG+   QDA +L ++++ L +A   DLR       G R ++    + +  E LG   +
Sbjct: 137 TFVGIWNSQDAGKLKRSKHELEIAVLLDLRKFVSDSSG-RTMKRCSFEKIVEENLGHSGV 196

Query: 199 SKPRNVTLSRWDREWLNPAQVLYATVDAF 200
              R V+ S W    L   Q+L A++D +
Sbjct: 197 RLDRKVSRSDWRVYDLRYVQILQASIDVY 224

[Close]

BLAST of Lag0026294 vs. TAIR 10
Match: AT3G12410.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 101.3 bits (251), Expect = 9.8e-22
Identity = 64/212 (30.19%), Postives = 106/212 (50.00%), Query Frame = 0


Query: 13  FDSHDYYDISFDDDDILTLLTACPSMVDLWISEILRIHRRRLHRLVVGLDIEW------- 72
           +++H  Y + F  D+ +  +T   S++  WI  +L  +R   H LVVG+ ++W       
Sbjct: 12  YNTHLEYSVDFFGDEFIVTVTWDSSVISRWIRNVLFNNRFSSHPLVVGVGVQWTPFSYYS 71

Query: 73  --RPN--------FNRNHHNPVATLQLCVGRRCLIFQLIHAPEIPDSLIEFLEDESFTFV 132
             RPN              NP   LQLCVG RCLI QL +  ++P++L  FL D   TFV
Sbjct: 72  DPRPNNYYADPPPIRYYSDNPADILQLCVGNRCLIIQLGYCDQVPNNLRSFLADPETTFV 131

Query: 133 GVGVDQDARRLQN-EYGLNVANTADLRDLAESEMGRRDLRNAGLKDLGREILGRE-ISKP 192
           GV   QDA +L    + L +    D+R       G R +R +  +++  E +G + +   
Sbjct: 132 GVWNGQDAGKLARCCHQLEIGELLDIRRYVTDSWG-RSMRRSSFEEIVEECMGYQGVMLD 191

Query: 193 RNVTLSRWDREWLNPAQVLYATVDAFLSFEIG 206
             +++S W    L+  Q+L A++DA++  ++G
Sbjct: 192 PEISMSDWTAYDLDLDQILQASLDAYVCHQLG 222

[Close]

BLAST of Lag0026294 vs. TAIR 10
Match: AT4G13870.2 (Werner syndrome-like exonuclease )

HSP 1 Score: 100.1 bits (248), Expect = 2.2e-21
Identity = 54/152 (35.53%), Postives = 88/152 (57.89%), Query Frame = 0


Query: 59  VGLDIEWRPNFNRN-HHNPVATLQLCV-GRRCLIFQLIHAPEIPDSLIEFLEDESFTFVG 118
           VGLDIEWRP+F +      VAT+Q+CV    C +  + H+  IP SL   +ED +   VG
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHS-GIPQSLQHLIEDSTLVKVG 189

Query: 119 VGVDQDARRLQNEYGLNVANTADLRDLAESEMGRRDLRNAGLKDLGREILGREISKPRNV 178
           +G+D D+ +L ++YG+++ +  DL DLA  ++G    +  GL  L   ++ +E+ KP  +
Sbjct: 190 IGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGLASLTETLVCKELLKPNRI 249

Query: 179 TLSRWDREWLNPAQVLYATVDAFLSFEIGRYL 209
            L  W+   L+  Q+ YA  DA+ S+ + + L
Sbjct: 250 RLGNWEFYPLSKQQLQYAATDAYASWHLYKVL 278

The following BLAST results are available for this feature:

BLAST of Lag0026294 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136771.1	1.3e-92	79.05	Werner Syndrome-like exonuclease [Cucumis sativus]	[more]
XP_038906336.1	1.9e-91	79.52	Werner Syndrome-like exonuclease [Benincasa hispida]	[more]
KAE8651170.1	9.3e-91	79.13	hypothetical protein Csa_000755 [Cucumis sativus]	[more]
XP_022147765.1	1.9e-75	71.57	Werner Syndrome-like exonuclease, partial [Momordica charantia]	[more]
KAB1222804.1	6.1e-74	66.83	Werner Syndrome-like exonuclease [Morella rubra]	[more]

BLAST of Lag0026294 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84LH3	3.1e-20	35.53	Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=...	[more]
Q9VGN7	1.3e-15	34.76	Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227...	[more]
O93530	3.3e-14	30.26	Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn ...	[more]
Q9NVH0	1.2e-11	31.21	Exonuclease 3'-5' domain-containing protein 2 OS=Homo sapiens OX=9606 GN=EXD2 PE...	[more]
Q8VEG4	1.3e-10	31.85	Exonuclease 3'-5' domain-containing protein 2 OS=Mus musculus OX=10090 GN=Exd2 P...	[more]

BLAST of Lag0026294 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHC5	6.3e-93	79.05	3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A6J1D121	9.1e-76	71.57	Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016622 ...	[more]
A0A6A1WBZ0	2.9e-74	66.83	Werner Syndrome-like exonuclease OS=Morella rubra OX=262757 GN=CJ030_MR2G022295 ...	[more]
A0A2I4GID7	5.0e-74	67.32	Werner Syndrome-like exonuclease OS=Juglans regia OX=51240 GN=LOC109008130 PE=4 ...	[more]
A0A5C7ILY4	9.4e-73	64.08	3'-5' exonuclease domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV...	[more]

BLAST of Lag0026294 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12460.1	1.5e-22	30.62	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G36110.1	4.4e-22	33.68	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G12430.1	7.5e-22	30.14	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G12410.1	9.8e-22	30.19	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G13870.2	2.2e-21	35.53	Werner syndrome-like exonuclease	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002562	3'-5' exonuclease domain	PFAM	PF01612	DNA_pol_A_exo1	coord: 54..208 e-value: 9.7E-14 score: 51.5
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 18..209 e-value: 4.5E-59 score: 201.3
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 10..209
None	No IPR available	PANTHER	PTHR13620:SF65	OS01G0660800 PROTEIN	coord: 10..209
None	No IPR available	CDD	cd06141	WRN_exo	coord: 33..209 e-value: 5.23092E-64 score: 193.949
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 42..208

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0026294.1	Lag0026294.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006139	nucleobase-containing compound metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding