Lag0026262 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0026262
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Reverse transcriptase domain-containing protein
Location	chr10: 33542243 .. 33543514 (+)
RNA-Seq Expression	Lag0026262
Synteny	Lag0026262

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGCAAGAGGATACACAAATCATGGTCAGCCAACTAGGAAAAAGACATGGGCTAGAATCTTCTGTGGAATGTGACACCAACAAGAAACTGAAAACTAAATGCTTCGAGGAAATCAGAGGGATATCGGCGGAGGCTGTGGATCAGCCCGTCGGACGCATGAAAACATTATGCTGGAACGCTCGAGGATTGGGGAATCCTCGAGCGATCCGAGCGCTCCGCCATTTTGTTGGAAGCTTAAATCCCCACTTGATCTTCATCATGGAAACGAAATGTGACTCTGGGAAATGTGATAGAATAAAAGCTAGTTTACAATATGACTTTGTGTTCAGTGTGCCCAGCAAAGGTAGTAGTGGAGGGCTTATGATCCTTTGGAATTCGGAGACTGAGGTTTTGATTAGCTCCTATTTAGAGGGGCATATTGACACTATGATCAAAGATAAGTTGGGTACTTGGCGCTTCACAGGGTTTATGGAAATCCTGAAACAGATAAAAGGCGCTTCTCTTGGGATTTGTTAGAAAGACTTAAGCTGTGTTATGAGGGTCCGTGGCTCGTGGGGGTGACTTCAATGAAGTGATGTCGGTGGAGGAAAAGAAAGGAAGGAAGATTAGAAACCAAAACCAATTAGGCCTTTTTGGAAGCGCTGAATAGATGTGGCTTAAGGGATGCAGTAACTCAGGTGATAAATTCACTTGGAGAAGAGGCAAGATGGAGCGTAATCAAATTAAGAAAGATTGGACAGGTATTTCTTTAATTACCCCATGTCTATCTTGGCCCCAATATCAAGATTAACCACCTAAACTTCCATAGCTCAGACCATCGGCCTATAACGACTCAGTGGGAGATTCAGGCTGTTCACAGCAGGGTTCTGCCAAATAAGAAGCTGCTGCATTTTGAAGAAGGCTGGATCAAACTGAAAGACACAAGAATATTATTGAGGAAGAATGGAGGAAGTCGAATGGCTGTGGGGCTCAGAACTTTAGTGCAAAAATCCTTAGATGCATCCACAAGCTGCACAAATGGAAAAATCAAGGTTGAAAGGCAGCTTGAAACAAGCCATCAAGAGGAAAGAAGACGAAGTTCAGGCTTTATACGCGGTTGAATCTGACCAGAATTGGGAGAGTATACTGAATGCAGAATCCGAGCTCGATGACCTCCTTGAGGAAGAAGAAGAATATTGGCGGATCCGTTCTAGAGATGATTGGTTAAAAAGCGGAGACAGAACACAAAATGGTTCCATGCAAAAGCTTCTCAACGAAAAAAAGAAATAG

mRNA sequence

ATGCAGCAAGAGGATACACAAATCATGGTCAGCCAACTAGGAAAAAGACATGGGCTAGAATCTTCTGTGGAATGTGACACCAACAAGAAACTGAAAACTAAATGCTTCGAGGAAATCAGAGGGATATCGGCGGAGGCTGTGGATCAGCCCGTCGGACGCATGAAAACATTATGCTGGAACGCTCGAGGATTGGGGAATCCTCGAGCGATCCGAGCGCTCCGCCATTTTGTTGGAAGCTTAAATCCCCACTTGATCTTCATCATGGAAACGAAATGTGACTCTGGGAAATGTGATAGAATAAAAGCTAGTTTACAATATGACTTTGTGTTCAGTGTGCCCAGCAAAGGTAGTAGTGGAGGGCTTATGATCCTTTGGAATTCGGAGACTGAGGTTTTGATTAGCTCCTATTTAGAGGGGCATATTGACACTATGATCAAAGATAAGTTGGGTACTTGGCGCTTCACAGGGTTTATGGAAATCCTGAAACAGATAAAAGGCGCTTCTCTTGGGATTTTAACTCAGGTGATAAATTCACTTGGAGAAGAGGCAAGATGGAGCGTAATCAAATTAAGAAAGATTGGACAGATGCATCCACAAGCTGCACAAATGGAAAAATCAAGGTTGAAAGGCAGCTTGAAACAAGCCATCAAGAGGAAAGAAGACGAAGTTCAGGCTTTATACGCGGTTGAATCTGACCAGAATTGGGAGAGTATACTGAATGCAGAATCCGAGCTCGATGACCTCCTTGAGGAAGAAGAAGAATATTGGCGGATCCGTTCTAGAGATGATTGGTTAAAAAGCGGAGACAGAACACAAAATGGTTCCATGCAAAAGCTTCTCAACGAAAAAAAGAAATAG

Coding sequence (CDS)

ATGCAGCAAGAGGATACACAAATCATGGTCAGCCAACTAGGAAAAAGACATGGGCTAGAATCTTCTGTGGAATGTGACACCAACAAGAAACTGAAAACTAAATGCTTCGAGGAAATCAGAGGGATATCGGCGGAGGCTGTGGATCAGCCCGTCGGACGCATGAAAACATTATGCTGGAACGCTCGAGGATTGGGGAATCCTCGAGCGATCCGAGCGCTCCGCCATTTTGTTGGAAGCTTAAATCCCCACTTGATCTTCATCATGGAAACGAAATGTGACTCTGGGAAATGTGATAGAATAAAAGCTAGTTTACAATATGACTTTGTGTTCAGTGTGCCCAGCAAAGGTAGTAGTGGAGGGCTTATGATCCTTTGGAATTCGGAGACTGAGGTTTTGATTAGCTCCTATTTAGAGGGGCATATTGACACTATGATCAAAGATAAGTTGGGTACTTGGCGCTTCACAGGGTTTATGGAAATCCTGAAACAGATAAAAGGCGCTTCTCTTGGGATTTTAACTCAGGTGATAAATTCACTTGGAGAAGAGGCAAGATGGAGCGTAATCAAATTAAGAAAGATTGGACAGATGCATCCACAAGCTGCACAAATGGAAAAATCAAGGTTGAAAGGCAGCTTGAAACAAGCCATCAAGAGGAAAGAAGACGAAGTTCAGGCTTTATACGCGGTTGAATCTGACCAGAATTGGGAGAGTATACTGAATGCAGAATCCGAGCTCGATGACCTCCTTGAGGAAGAAGAAGAATATTGGCGGATCCGTTCTAGAGATGATTGGTTAAAAAGCGGAGACAGAACACAAAATGGTTCCATGCAAAAGCTTCTCAACGAAAAAAAGAAATAG

Protein sequence

MQQEDTQIMVSQLGKRHGLESSVECDTNKKLKTKCFEEIRGISAEAVDQPVGRMKTLCWNARGLGNPRAIRALRHFVGSLNPHLIFIMETKCDSGKCDRIKASLQYDFVFSVPSKGSSGGLMILWNSETEVLISSYLEGHIDTMIKDKLGTWRFTGFMEILKQIKGASLGILTQVINSLGEEARWSVIKLRKIGQMHPQAAQMEKSRLKGSLKQAIKRKEDEVQALYAVESDQNWESILNAESELDDLLEEEEEYWRIRSRDDWLKSGDRTQNGSMQKLLNEKKK

Homology

[Close]

BLAST of Lag0026262 vs. NCBI nr
Match: XP_022157437.1 (uncharacterized protein LOC111024135 [Momordica charantia])

HSP 1 Score: 117.9 bits (294), Expect = 1.5e-22
Identity = 55/104 (52.88%), Postives = 67/104 (64.42%), Query Frame = 0


Query: 54  MKTLCWNARGLGNPRAIRALRHFVGSLNPHLIFIMETKCDSGKCDRIKASLQYDFVFSVP 113
           MK+LCWN  GLGNP   R LR+ V    P L+F+ ETK +     R K  L +D   SV 
Sbjct: 1   MKSLCWNVPGLGNPDTFRTLRNLVRRSQPQLVFLSETKGNPTLEGRAKRELSFDCCVSVA 60

Query: 114 SKGSSGGLMILWNSETEVLISSYLEGHIDTMIKDKLGTWRFTGF 158
           S G SGGLM+LWNS++ V I S   GHID++I DK G+WRFTGF
Sbjct: 61  STGKSGGLMLLWNSDSNVRIQSMSPGHIDSIITDKYGSWRFTGF 104

[Close]

BLAST of Lag0026262 vs. NCBI nr
Match: KAA3453480.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 109.8 bits (273), Expect = 4.0e-20
Identity = 50/106 (47.17%), Postives = 70/106 (66.04%), Query Frame = 0


Query: 54  MKTLCWNARGLGNPRAIRALRHFVGSLNPHLIFIMETKCDSGKCDRIKASLQYDFVFSVP 113
           MK  CWNARGLG+PRA+R LR+ +   +PHL+F+METK DS   DR++ S  +    +V 
Sbjct: 1   MKHFCWNARGLGSPRAVRRLRYILKQHHPHLVFLMETKLDSKCMDRVRRSCGFTNGINVE 60

Query: 114 SKGSSGGLMILWNSETEVLISSYLEGHIDTMIKDK--LGTWRFTGF 158
           ++GS GGL + W  +  V + SY + HID +IK++     WRFTGF
Sbjct: 61  AEGSRGGLCLAWRDDIGVTLRSYSKWHIDVLIKEEGIQEEWRFTGF 106

[Close]

BLAST of Lag0026262 vs. NCBI nr
Match: XP_042950313.1 (uncharacterized protein LOC122282426 [Carya illinoinensis])

HSP 1 Score: 109.4 bits (272), Expect = 5.2e-20
Identity = 53/104 (50.96%), Postives = 67/104 (64.42%), Query Frame = 0


Query: 54  MKTLCWNARGLGNPRAIRALRHFVGSLNPHLIFIMETKCDSGKCDRIKASLQYDFVFSVP 113
           MKT+CWN+RGLGNP  IRALR  +    P L+F+ ETK  +   D +K  L +   FSV 
Sbjct: 1   MKTICWNSRGLGNPSGIRALRDLIAREGPDLLFLQETKLSTKGMDALKCKLGFVNCFSVD 60

Query: 114 SKGSSGGLMILWNSETEVLISSYLEGHIDTMIK-DKLGTWRFTG 157
           S+G SGGL +LWNS+  V + S+ + HID  IK D L  WRFTG
Sbjct: 61  SEGRSGGLGLLWNSDLGVELRSFSQYHIDVHIKIDDLMVWRFTG 104

[Close]

BLAST of Lag0026262 vs. NCBI nr
Match: XP_030505962.1 (uncharacterized protein LOC115720894 [Cannabis sativa])

HSP 1 Score: 107.8 bits (268), Expect = 1.5e-19
Identity = 83/245 (33.88%), Postives = 115/245 (46.94%), Query Frame = 0


Query: 54  MKTLCWNARGLGNPRAIRALRHFVGSLNPHLIFIMETKCDSGKCDRIKASLQYDFVFSVP 113
           M  + WNARGLGNP A R L   V   NP L+F+METK  +G   R K SL +     V 
Sbjct: 1   MNLISWNARGLGNPSAFRRLWLLVRKYNPQLLFLMETKATTGSLVRFKQSLNFCNGLEVA 60

Query: 114 SKGSSGGLMILWNSETEVLISSYLEGHIDTMIKDKLG-TWRFT---GFME-ILKQIKGAS 173
             G  GG+M+LW     V + S    H D     + G  W F+   GF E + K+     
Sbjct: 61  RVGLKGGIMLLWKETVNVSLLSMNTNHFDCFGSYEDGPRWHFSAVYGFPESVNKKFTWEL 120

Query: 174 LGILTQVI--------NSLGEEARWSVIKLR-KIGQMHPQAAQME------------KSR 233
           +  L  V         ++L E   W +I  +   G M P+   ++             +R
Sbjct: 121 INRLVDVSPMDPWKNPSALKERLDWCLINQKWTDGLMIPKLTHLDYFGSDHRALLSWHTR 180

Query: 234 LKGSLKQAIKRKEDEVQALYAVESDQ--NWESILNAESELDDLLEEEEEYWRIRSRDDWL 271
             G LK  I + +  V  +  + S    +  ++  AES LDDLL +EE+YW+ RSR DWL
Sbjct: 181 KFGQLKSDISQAQSRVNHINNLPSPTLLHSHTLNTAESILDDLLAKEEQYWQQRSRIDWL 240

[Close]

BLAST of Lag0026262 vs. NCBI nr
Match: KAA3467350.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 107.5 bits (267), Expect = 2.0e-19
Identity = 46/104 (44.23%), Postives = 68/104 (65.38%), Query Frame = 0


Query: 54  MKTLCWNARGLGNPRAIRALRHFVGSLNPHLIFIMETKCDSGKCDRIKASLQYDFVFSVP 113
           MK++CWN RGLG+PRA+R LRH +   NPHL+F+METK D  + +R++    +     V 
Sbjct: 1   MKSICWNVRGLGSPRAVRRLRHLIKQHNPHLVFLMETKLDKHRMERVRRRCGFLCGVEVE 60

Query: 114 SKGSSGGLMILWNSETEVLISSYLEGHIDTMIK--DKLGTWRFT 156
           ++GS GGL + W  + +V + S+ + HID +IK  D +  WRFT
Sbjct: 61  AEGSRGGLCLAWKKDIQVNLRSFSKWHIDVIIKEDDMVSEWRFT 104

[Close]

BLAST of Lag0026262 vs. ExPASy TrEMBL
Match: A0A6J1DUG8 (uncharacterized protein LOC111024135 OS=Momordica charantia OX=3673 GN=LOC111024135 PE=4 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 7.1e-23
Identity = 55/104 (52.88%), Postives = 67/104 (64.42%), Query Frame = 0


Query: 54  MKTLCWNARGLGNPRAIRALRHFVGSLNPHLIFIMETKCDSGKCDRIKASLQYDFVFSVP 113
           MK+LCWN  GLGNP   R LR+ V    P L+F+ ETK +     R K  L +D   SV 
Sbjct: 1   MKSLCWNVPGLGNPDTFRTLRNLVRRSQPQLVFLSETKGNPTLEGRAKRELSFDCCVSVA 60

Query: 114 SKGSSGGLMILWNSETEVLISSYLEGHIDTMIKDKLGTWRFTGF 158
           S G SGGLM+LWNS++ V I S   GHID++I DK G+WRFTGF
Sbjct: 61  STGKSGGLMLLWNSDSNVRIQSMSPGHIDSIITDKYGSWRFTGF 104

[Close]

BLAST of Lag0026262 vs. ExPASy TrEMBL
Match: A0A2N9G1F9 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS21082 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 2.3e-21
Identity = 51/109 (46.79%), Postives = 69/109 (63.30%), Query Frame = 0


Query: 50  PVGRMKTLCWNARGLGNPRAIRALRHFVGSLNPHLIFIMETKCDSGKCDRIKASLQYDFV 109
           P   M  L WN +GLGNP A+RAL H V    P ++F+METK D+G+ + I+  L +D  
Sbjct: 482 PPETMILLSWNCQGLGNPEAVRALHHMVKKKGPEVLFLMETKLDAGRMEVIRVKLGFDNT 541

Query: 110 FSVPSKGSSGGLMILWNSETEVLISSYLEGHIDTMIKDKLG-TWRFTGF 158
           F+VPS G SGGL +LW ++ EV+I +Y + HID  +  K    WR TGF
Sbjct: 542 FTVPSLGRSGGLALLWKADAEVVIQNYSQHHIDAHVDSKKAKCWRLTGF 590

[Close]

BLAST of Lag0026262 vs. ExPASy TrEMBL
Match: A0A2N9G8I6 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS23296 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 2.3e-21
Identity = 51/109 (46.79%), Postives = 69/109 (63.30%), Query Frame = 0


Query: 50  PVGRMKTLCWNARGLGNPRAIRALRHFVGSLNPHLIFIMETKCDSGKCDRIKASLQYDFV 109
           P   M  L WN +GLGNP A+RAL H V    P ++F+METK D+G+ + I+  L +D  
Sbjct: 477 PPETMILLSWNCQGLGNPEAVRALHHMVKKKGPEVLFLMETKLDAGRMEVIRVKLGFDNT 536

Query: 110 FSVPSKGSSGGLMILWNSETEVLISSYLEGHIDTMIKDKLG-TWRFTGF 158
           F+VPS G SGGL +LW ++ EV+I +Y + HID  +  K    WR TGF
Sbjct: 537 FTVPSLGRSGGLALLWKADAEVVIQNYSQHHIDAHVDSKQAKCWRLTGF 585

[Close]

BLAST of Lag0026262 vs. ExPASy TrEMBL
Match: A0A2N9IBI9 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS49113 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 2.3e-21
Identity = 51/109 (46.79%), Postives = 69/109 (63.30%), Query Frame = 0


Query: 50  PVGRMKTLCWNARGLGNPRAIRALRHFVGSLNPHLIFIMETKCDSGKCDRIKASLQYDFV 109
           P   M  L WN +GLGNP A+RAL H V    P ++F+METK D+G+ + I+  L +D  
Sbjct: 166 PPETMILLSWNCQGLGNPEAVRALHHMVKKKGPEVLFLMETKLDAGRMEVIRVKLGFDNT 225

Query: 110 FSVPSKGSSGGLMILWNSETEVLISSYLEGHIDTMIKDKLG-TWRFTGF 158
           F+VPS G SGGL +LW ++ EV+I +Y + HID  +  K    WR TGF
Sbjct: 226 FTVPSLGRSGGLALLWKADAEVVIQNYSQHHIDAHVDSKQAKCWRLTGF 274

[Close]

BLAST of Lag0026262 vs. ExPASy TrEMBL
Match: A0A803Q6Y1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 3.0e-21
Identity = 86/269 (31.97%), Postives = 118/269 (43.87%), Query Frame = 0


Query: 42  ISAEAVDQPVGRMKTLCWNARGLGNPRAIRALRHFVGSLNPHLIFIMETKCDSGKCDRIK 101
           ++  A+  P   MK L WNA GLGNP A R LR  V    PH++FIMETK D     R +
Sbjct: 466 MTTTALYAPQIAMKILSWNACGLGNPSAFRQLRLLVTEQTPHVLFIMETKLDCNVVTRYR 525

Query: 102 ASLQYDFVFSVPSKGSSGGLMILWNSETEVLISSYLEGHIDTMIKDKLG-TWRFTGFMEI 161
            +L++     VP  G SGGL++LW    +V + +Y     D  IK   G +W F+ F   
Sbjct: 526 QALRFSNGLEVPRVGLSGGLLLLWKDNVDVTLLNYNLNIFDCYIKSDNGQSWHFSAFYGA 585

Query: 162 LKQIKGASLGILTQVINSLGEEARWSVI--------KLRKIGQMHPQAAQMEKSR----- 221
            +  K      L +    +     W VI           K+G       QM+K R     
Sbjct: 586 PETHKRIHTWTLLERCKDVAPLMPWLVIGDFNEILSNDNKLGGALRNEQQMDKFRSVLDT 645

Query: 222 ------------------------LKGSLKQAIKRKEDEVQALYAVESDQNWESIL---N 270
                                     G++K+ IK    EV+ L   ++  N ES+     
Sbjct: 646 CYLYEQPFEGDPFTWIKGRHNHNMKYGNIKKKIKAAHKEVELLNNQQTRTN-ESVCKLKK 705

The following BLAST results are available for this feature:

BLAST of Lag0026262 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157437.1	1.5e-22	52.88	uncharacterized protein LOC111024135 [Momordica charantia]	[more]
KAA3453480.1	4.0e-20	47.17	reverse transcriptase [Gossypium australe]	[more]
XP_042950313.1	5.2e-20	50.96	uncharacterized protein LOC122282426 [Carya illinoinensis]	[more]
XP_030505962.1	1.5e-19	33.88	uncharacterized protein LOC115720894 [Cannabis sativa]	[more]
KAA3467350.1	2.0e-19	44.23	reverse transcriptase [Gossypium australe]	[more]

BLAST of Lag0026262 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Lag0026262 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DUG8	7.1e-23	52.88	uncharacterized protein LOC111024135 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A2N9G1F9	2.3e-21	46.79	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS21082 PE=4 SV=1	[more]
A0A2N9G8I6	2.3e-21	46.79	Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F...	[more]
A0A2N9IBI9	2.3e-21	46.79	Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F...	[more]
A0A803Q6Y1	3.0e-21	31.97	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]

BLAST of Lag0026262 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036691	Endonuclease/exonuclease/phosphatase superfamily	GENE3D	3.60.10.10	Endonuclease/exonuclease/phosphatase	coord: 52..160 e-value: 2.7E-11 score: 45.7
IPR036691	Endonuclease/exonuclease/phosphatase superfamily	SUPERFAMILY	56219	DNase I-like	coord: 53..130
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 265..285

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0026262.1	Lag0026262.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090304	nucleic acid metabolic process
molecular_function	GO:0003824	catalytic activity