Lag0025825 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0025825
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionolee1-like protein
Locationchr10: 21615245 .. 21615732 (+)
RNA-Seq ExpressionLag0025825
SyntenyLag0025825
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAATCCGCAATCCTACTTGTTTCAGCCCTTTGCTTCATGTCGCTCCTTAACCTCACATTCTACGAGAAGGATCGGTTATTCGTCGAGGGGAAAGTGTATTGCGACACTTGCCGCATCCAATTCTTCACCAAAGCCAACAAATTCCTCGAAGGTAAAAAGCCCTAACAATCCTAATCAAAAGCCCTAACTAACTGACGATCTTAATTCGAAATCAATTGAAATTGAAATTGAATGCAGGTGCGACGGTGAAATTGGTATGCAAAGACGGGGAAGGGGGAAGTGAGACGTTCAGCAACAAAGTGGTGATGGATAAGACGGGATCTTACAGCATGGAGGTGGACGGAGACCATGAGGAGGAGGTGTGCGAGGTGTCGCTGGTGAAGAGCAAGGATTCGGTTTTTAGAGCGAGAGAATCTGCAATTGGGGTTTTGGGTTATGGGGAGAAAAATGAAGGGAGAAGGGGAGTAGAAGTATTTTGTTAA

mRNA sequence

ATGGCGAAATCCGCAATCCTACTTGTTTCAGCCCTTTGCTTCATGTCGCTCCTTAACCTCACATTCTACGAGAAGGATCGGTTATTCGTCGAGGGGAAAGTGTATTGCGACACTTGCCGCATCCAATTCTTCACCAAAGCCAACAAATTCCTCGAAGGTGCGACGGTGAAATTGGTATGCAAAGACGGGGAAGGGGGAAGTGAGACGTTCAGCAACAAAGTGGTGATGGATAAGACGGGATCTTACAGCATGGAGGTGGACGGAGACCATGAGGAGGAGGTGTGCGAGGTGTCGCTGGTGAAGAGCAAGGATTCGGTTTTTAGAGCGAGAGAATCTGCAATTGGGGTTTTGGGTTATGGGGAGAAAAATGAAGGGAGAAGGGGAGTAGAAGTATTTTGTTAA

Coding sequence (CDS)

ATGGCGAAATCCGCAATCCTACTTGTTTCAGCCCTTTGCTTCATGTCGCTCCTTAACCTCACATTCTACGAGAAGGATCGGTTATTCGTCGAGGGGAAAGTGTATTGCGACACTTGCCGCATCCAATTCTTCACCAAAGCCAACAAATTCCTCGAAGGTGCGACGGTGAAATTGGTATGCAAAGACGGGGAAGGGGGAAGTGAGACGTTCAGCAACAAAGTGGTGATGGATAAGACGGGATCTTACAGCATGGAGGTGGACGGAGACCATGAGGAGGAGGTGTGCGAGGTGTCGCTGGTGAAGAGCAAGGATTCGGTTTTTAGAGCGAGAGAATCTGCAATTGGGGTTTTGGGTTATGGGGAGAAAAATGAAGGGAGAAGGGGAGTAGAAGTATTTTGTTAA

Protein sequence

MAKSAILLVSALCFMSLLNLTFYEKDRLFVEGKVYCDTCRIQFFTKANKFLEGATVKLVCKDGEGGSETFSNKVVMDKTGSYSMEVDGDHEEEVCEVSLVKSKDSVFRARESAIGVLGYGEKNEGRRGVEVFC
Homology
BLAST of Lag0025825 vs. NCBI nr
Match: XP_008467237.1 (PREDICTED: olee1-like protein [Cucumis melo] >KAA0055160.1 olee1-like protein [Cucumis melo var. makuwa] >TYK00303.1 olee1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 159.5 bits (402), Expect = 2.1e-35
Identity = 79/104 (75.96%), Postives = 88/104 (84.62%), Query Frame = 0

Query: 1   MAKSAILLVSALCFMSLLNLTFYEKDRLFVEGKVYCDTCRIQFFTKANKFLEGATVKLVC 60
           MAKS ILLVSAL  MSLLN+ F +KDR  VEGKVYCD CR+QFFTKA+KFLEGATVKLVC
Sbjct: 1   MAKSMILLVSALSLMSLLNVAFSQKDRFLVEGKVYCDACRVQFFTKASKFLEGATVKLVC 60

Query: 61  KDGEGGSETFSNKVVMDKTGSYSMEVDGDHEEEVCEVSLVKSKD 105
           K+ EGGS T S + V DKTGSYS+E DGDHEEE+CEV+LVKS D
Sbjct: 61  KEIEGGSVTLSKEAVTDKTGSYSIEADGDHEEELCEVTLVKSND 104

BLAST of Lag0025825 vs. NCBI nr
Match: XP_022933305.1 (olee1-like protein [Cucurbita moschata] >XP_022968405.1 olee1-like protein [Cucurbita maxima] >XP_023529885.1 olee1-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 158.3 bits (399), Expect = 4.6e-35
Identity = 77/104 (74.04%), Postives = 88/104 (84.62%), Query Frame = 0

Query: 1   MAKSAILLVSALCFMSLLNLTFYEKDRLFVEGKVYCDTCRIQFFTKANKFLEGATVKLVC 60
           MAKSA++LVSALC MSL+N+   EK+R FVEGKVYCDTCRIQFFTK +KFLEGATVKL C
Sbjct: 1   MAKSAVMLVSALCLMSLINIATCEKERFFVEGKVYCDTCRIQFFTKVSKFLEGATVKLEC 60

Query: 61  KDGEGGSETFSNKVVMDKTGSYSMEVDGDHEEEVCEVSLVKSKD 105
           +D EGG+ TFS +   DK+GSY +E D DHEEEVCEVSLVKSKD
Sbjct: 61  RDIEGGNVTFSKETETDKSGSYKIETDKDHEEEVCEVSLVKSKD 104

BLAST of Lag0025825 vs. NCBI nr
Match: KAG6588099.1 (hypothetical protein SDJN03_16664, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 158.3 bits (399), Expect = 4.6e-35
Identity = 77/104 (74.04%), Postives = 88/104 (84.62%), Query Frame = 0

Query: 1   MAKSAILLVSALCFMSLLNLTFYEKDRLFVEGKVYCDTCRIQFFTKANKFLEGATVKLVC 60
           MAKSA++LVSALC MSL+N+   EK+R FVEGKVYCDTCRIQFFTK +KFLEGATVKL C
Sbjct: 1   MAKSAVMLVSALCLMSLINIATCEKERFFVEGKVYCDTCRIQFFTKVSKFLEGATVKLEC 60

Query: 61  KDGEGGSETFSNKVVMDKTGSYSMEVDGDHEEEVCEVSLVKSKD 105
           +D EGG+ TFS +   DK+GSY +E D DHEEEVCEVSLVKSKD
Sbjct: 61  RDIEGGNVTFSKETETDKSGSYKIETDKDHEEEVCEVSLVKSKD 104

BLAST of Lag0025825 vs. NCBI nr
Match: XP_022988307.1 (olee1-like protein [Cucurbita maxima])

HSP 1 Score: 157.5 bits (397), Expect = 7.8e-35
Identity = 76/105 (72.38%), Postives = 89/105 (84.76%), Query Frame = 0

Query: 1   MAKSAILLVSALCFMSLLNLTFYEKDRLFVEGKVYCDTCRIQFFTKANKFLEGATVKLVC 60
           MAKSAI+LVSALCFMSLLNL   +KDR FVEGKVYCDTCR+QFFTK +KF+EGA VKL C
Sbjct: 1   MAKSAIMLVSALCFMSLLNLAACKKDRFFVEGKVYCDTCRVQFFTKVSKFMEGAKVKLEC 60

Query: 61  KDGEGGSETFSNKVVMDKTGSYSMEVDGDHEEEVCEVSLVKSKDS 106
           ++ EGG+ T + + V DKTGSY++E DGDHEE+VCEVSL KS DS
Sbjct: 61  REIEGGNVTLNKEAVTDKTGSYNIEADGDHEEDVCEVSLEKSSDS 105

BLAST of Lag0025825 vs. NCBI nr
Match: XP_038880683.1 (olee1-like protein [Benincasa hispida])

HSP 1 Score: 155.6 bits (392), Expect = 3.0e-34
Identity = 76/104 (73.08%), Postives = 87/104 (83.65%), Query Frame = 0

Query: 1   MAKSAILLVSALCFMSLLNLTFYEKDRLFVEGKVYCDTCRIQFFTKANKFLEGATVKLVC 60
           MAK AI+LVS LCF+SL +L    KD+ FVEGKVYCDTCR+QFFTK +KFLEGATVKL C
Sbjct: 1   MAKFAIILVSTLCFISLFHLVSCNKDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLEC 60

Query: 61  KDGEGGSETFSNKVVMDKTGSYSMEVDGDHEEEVCEVSLVKSKD 105
           K+ EGGS T S + V DKTG+YS+E DGDHEEEVCEVSLVKS+D
Sbjct: 61  KEIEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSED 104

BLAST of Lag0025825 vs. ExPASy Swiss-Prot
Match: O49813 (Olee1-like protein OS=Betula pendula OX=3505 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 1.5e-25
Identity = 56/104 (53.85%), Postives = 74/104 (71.15%), Query Frame = 0

Query: 1   MAKSAILLVSALCFMSLLNLTFYEKDRLFVEGKVYCDTCRIQFFTKANKFLEGATVKLVC 60
           MAKS I+   ALCF+SLL    Y + R FVEGKVYCD CR QF TK + +++GA V L C
Sbjct: 1   MAKSIIIQAPALCFLSLLGFA-YSESRFFVEGKVYCDNCRTQFVTKLSTYMKGAKVSLEC 60

Query: 61  KDGEGGSETFSNKVVMDKTGSYSMEVDGDHEEEVCEVSLVKSKD 105
           ++ EGG+  +S+    DK+G+Y + VDGDHEEE+CE++L KS D
Sbjct: 61  RNREGGTLIYSSDSETDKSGTYRIPVDGDHEEEICEIALKKSSD 103

BLAST of Lag0025825 vs. ExPASy Swiss-Prot
Match: Q6NMJ2 (Pollen-specific protein-like At4g18596 OS=Arabidopsis thaliana OX=3702 GN=At4g18596 PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 6.5e-24
Identity = 59/110 (53.64%), Postives = 73/110 (66.36%), Query Frame = 0

Query: 1   MAKSAI--LLVSALCFMSLLNLTFYEK---DRLFVEGKVYCDTCRIQFFTKANKFLEGAT 60
           MA  AI    VSA+C  SL  +   +    DR  ++G VYCDTCR+QF T+ +KFLEGA 
Sbjct: 1   MASKAIFFFFVSAVCLSSLAGVAIADADDFDRFQIQGSVYCDTCRVQFVTRLSKFLEGAK 60

Query: 61  VKLVCKDGEGGSETFSNKVVMDKTGSYSMEVDGDHEEEVCEVSLVKSKDS 106
           VKL C+    G+ T + + V DKTGSY MEV GDHEEEVCE+ LV+S DS
Sbjct: 61  VKLECRSRTNGTVTLTKEAVTDKTGSYRMEVTGDHEEEVCELVLVESPDS 110

BLAST of Lag0025825 vs. ExPASy Swiss-Prot
Match: P13447 (Anther-specific protein LAT52 OS=Solanum lycopersicum OX=4081 GN=LAT52 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 5.5e-23
Identity = 56/102 (54.90%), Postives = 74/102 (72.55%), Query Frame = 0

Query: 1   MAKSAILLVSALCFMSLLNLTFYEKDRLFVEGKVYCDTCRIQFFTKANKFLEGATVKLVC 60
           MAK AI+L+SALC ++L N      +   VEGKVYCDTCR+QF TK ++ LEGATVKL C
Sbjct: 1   MAK-AIVLLSALCILALANFAHCRPEVFDVEGKVYCDTCRVQFETKLSENLEGATVKLQC 60

Query: 61  KDGEGGSETFSNKVVMDKTGSYSMEVDGDHEEEVCEVSLVKS 103
           ++    +ETFS + V DK G Y + V+GDHE ++CEV++VKS
Sbjct: 61  RNISTEAETFSVEGVTDKDGKYKLTVNGDHENDICEVTVVKS 101

BLAST of Lag0025825 vs. ExPASy Swiss-Prot
Match: Q8LGR0 (Pollen allergen Che a 1 OS=Chenopodium album OX=3559 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 4.6e-22
Identity = 49/103 (47.57%), Postives = 71/103 (68.93%), Query Frame = 0

Query: 3   KSAILLVSALCFMSLLNLTFYEKDRLFVEGKVYCDTCRIQFFTKANKFLEGATVKLVCKD 62
           ++  LLV ALC +SL  +    ++   V+G VYCDTCRIQF T+ +  +EGATVKL C++
Sbjct: 5   QAVFLLVGALCVLSLAGVANAAENHFKVQGMVYCDTCRIQFMTRISTIMEGATVKLECRN 64

Query: 63  GEGGSETFSNKVVMDKTGSYSMEVDGDHEEEVCEVSLVKSKDS 106
              G++TF  + V DK G YS+ V+GD E+++CE+ LVKS +S
Sbjct: 65  ITAGTQTFKAEAVTDKVGQYSIPVNGDFEDDICEIELVKSPNS 107

BLAST of Lag0025825 vs. ExPASy Swiss-Prot
Match: Q29W25 (Pollen allergen Cro s 1 OS=Crocus sativus OX=82528 PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.3e-21
Identity = 48/103 (46.60%), Postives = 70/103 (67.96%), Query Frame = 0

Query: 3   KSAILLVSALCFMSLLNLTFYEKDRLFVEGKVYCDTCRIQFFTKANKFLEGATVKLVCKD 62
           ++  LLV ALC +SL  +    ++   V+G VYCDTCRIQF T+ +  +EGATVKL C++
Sbjct: 5   QAVFLLVGALCVLSLAGVANAAENHFKVQGMVYCDTCRIQFMTRVSTIMEGATVKLECRN 64

Query: 63  GEGGSETFSNKVVMDKTGSYSMEVDGDHEEEVCEVSLVKSKDS 106
              G++TF  + V DK G YS+ V GD ++++CE+ LVKS +S
Sbjct: 65  ITAGTQTFKAEAVTDKVGQYSIPVHGDFQDDICEIELVKSPNS 107

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008467237.12.1e-3575.96PREDICTED: olee1-like protein [Cucumis melo] >KAA0055160.1 olee1-like protein [C... [more]
XP_022933305.14.6e-3574.04olee1-like protein [Cucurbita moschata] >XP_022968405.1 olee1-like protein [Cucu... [more]
KAG6588099.14.6e-3574.04hypothetical protein SDJN03_16664, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022988307.17.8e-3572.38olee1-like protein [Cucurbita maxima][more]
XP_038880683.13.0e-3473.08olee1-like protein [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O498131.5e-2553.85Olee1-like protein OS=Betula pendula OX=3505 PE=2 SV=1[more]
Q6NMJ26.5e-2453.64Pollen-specific protein-like At4g18596 OS=Arabidopsis thaliana OX=3702 GN=At4g18... [more]
P134475.5e-2354.90Anther-specific protein LAT52 OS=Solanum lycopersicum OX=4081 GN=LAT52 PE=2 SV=1[more]
Q8LGR04.6e-2247.57Pollen allergen Che a 1 OS=Chenopodium album OX=3559 PE=1 SV=1[more]
Q29W251.3e-2146.60Pollen allergen Cro s 1 OS=Crocus sativus OX=82528 PE=1 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF01190Pollen_Ole_e_1coord: 30..105
e-value: 6.3E-15
score: 55.4
NoneNo IPR availablePANTHERPTHR31614:SF2F28N24.16 PROTEINcoord: 3..104
IPR006041Pollen allergen Ole e 1 familyPANTHERPTHR31614PROTEIN DOWNSTREAM OF FLC-RELATEDcoord: 3..104
IPR006040Allergen Ole e 1, conserved sitePROSITEPS00925OLEEIcoord: 31..40

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0025825.1Lag0025825.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005615 extracellular space